CN111467433B - A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method - Google Patents

A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method Download PDF

Info

Publication number
CN111467433B
CN111467433B CN202010463029.9A CN202010463029A CN111467433B CN 111467433 B CN111467433 B CN 111467433B CN 202010463029 A CN202010463029 A CN 202010463029A CN 111467433 B CN111467433 B CN 111467433B
Authority
CN
China
Prior art keywords
parts
blood
wound healing
dragon
traditional chinese
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN202010463029.9A
Other languages
Chinese (zh)
Other versions
CN111467433A (en
Inventor
石珊
石继红
白晓智
官浩
胡大海
韩军涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Air Force Medical University of PLA
Original Assignee
Air Force Medical University of PLA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Air Force Medical University of PLA filed Critical Air Force Medical University of PLA
Priority to CN202010463029.9A priority Critical patent/CN111467433B/en
Publication of CN111467433A publication Critical patent/CN111467433A/en
Application granted granted Critical
Publication of CN111467433B publication Critical patent/CN111467433B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/30—Zinc; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/288—Taraxacum (dandelion)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A61K36/355—Lonicera (honeysuckle)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/739—Sanguisorba (burnet)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/85—Verbenaceae (Verbena family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/889—Arecaceae, Palmae or Palmaceae (Palm family), e.g. date or coconut palm or palmetto
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction

Abstract

The invention discloses an external traditional Chinese medicine composition and ointment for promoting wound healing and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine raw materials of the external traditional Chinese medicine composition comprise: 10-20 parts of pseudo-ginseng, 20-40 parts of dalbergia wood, 10-40 parts of beautyberry leaf, 15-30 parts of garden burnet root, 20-60 parts of pearl, 10-20 parts of bletilla, 20-30 parts of salvia miltiorrhiza, 10-30 parts of calcined calamine, 20-30 parts of dandelion, 10-20 parts of honeysuckle, 10-30 parts of cotton rose leaf, 10-30 parts of dragon's blood and 20-40 parts of ligusticum wallichii. The external traditional Chinese medicine composition has the effects of stopping bleeding, diminishing inflammation, relieving swelling and pain, removing blood stasis and promoting tissue regeneration, can effectively improve the blood circulation of local wound tissues, enhances the immunologic function and promotes the growth of granulation tissues, thereby promoting the rapid healing of the wound; meanwhile, the transdermal absorption effect of the medicine is good, the effective rate of the medicine is obviously improved, and the dosage of the medicine is reduced.

Description

A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to an external traditional Chinese medicine composition and ointment for promoting wound healing and a preparation method thereof.
Background
Wound healing is a general term for pathological processes caused after trauma, and is a complex and highly coordinated process involving multiple growth factors, cytokines, inflammatory cells and repair cells. Wound healing is a key problem in the field of surgery, and at present, research at home and abroad has been deeply carried out to the cell, molecule and gene level. The Chinese medicine is externally used for treating wounds, and has the functions of controlling local infection, protecting wound surfaces, accelerating wound healing and increasing wound healing strength. Modern medical research considers that the process of wound healing is very ordered, and is roughly divided into three stages of hemostasis and inflammation, proliferation and remodeling, which are mutually connected, but have characteristics and no obvious boundary.
Patent CN102813751B discloses a wound treatment agent, specifically discloses a traditional Chinese medicine preparation for promoting wound healing, which comprises the following traditional Chinese medicine raw materials: 10-30 parts of beautyberry leaf, 6-15 parts of dried sanguisorba or 30-90 parts of fresh product, 3-6 parts of dalbergia wood, 9-15 parts of dried corydalis bungeana or 30-60 parts of fresh product, 3-9 parts of myrrh, 6-12 parts of angelica, 6-12 parts of radix salviae miltiorrhizae, 15-25 parts of prepared rehmannia root, 6-12 parts of lithospermum and 15-30 parts of liquorice. The preparation has antibacterial, disinfectant and hemostatic effects at low concentration; can be used as medicine for promoting wound healing at higher concentration, and can promote wound healing rapidly. The inventor combines modern medical research with the theory of traditional Chinese medicine, and in the research and screening of the medicines, the inventor surprisingly discovers that the external traditional Chinese medicine preparation prepared by 13 traditional Chinese medicines such as pseudo-ginseng, dalbergia wood, garden burnet root, dandelion, cotton rose hibiscus leaf, ligusticum wallichii and the like can promote the wound to heal more quickly, has good transdermal absorption effect of the medicines, can obviously reduce the dosage of the medicines, and has the characteristic of remarkable curative effect.
Disclosure of Invention
The invention aims to: provides an external traditional Chinese medicine composition which has reasonable compatibility and good transdermal absorption effect and can promote wound healing more quickly and an ointment containing the traditional Chinese medicine composition.
In order to achieve the above purpose, the invention adopts the technical scheme that: the invention provides an external traditional Chinese medicine composition for promoting wound healing, which comprises the following components in parts by weight: 10-20 parts of pseudo-ginseng, 20-40 parts of dalbergia wood, 10-40 parts of beautyberry leaf, 15-30 parts of garden burnet root, 20-60 parts of pearl, 10-20 parts of bletilla, 20-30 parts of salvia miltiorrhiza, 10-30 parts of calcined calamine, 20-30 parts of dandelion, 10-20 parts of honeysuckle, 10-30 parts of cotton rose leaf, 10-30 parts of dragon's blood and 20-40 parts of ligusticum wallichii.
Preferably, the external traditional Chinese medicine composition comprises: 12-15 parts of pseudo-ginseng, 25-30 parts of dalbergia wood, 15-30 parts of beautyberry leaf, 20-25 parts of garden burnet root, 25-40 parts of pearl, 12-15 parts of bletilla, 22-25 parts of salvia miltiorrhiza, 15-25 parts of calcined calamine, 22-25 parts of dandelion, 12-15 parts of honeysuckle, 15-25 parts of cotton rose leaf, 15-25 parts of dragon's blood and 25-30 parts of ligusticum wallichii.
More preferably, the external traditional Chinese medicine composition comprises: 15 parts of pseudo-ginseng, 30 parts of dalbergia wood, 30 parts of beautyberry leaf, 25 parts of garden burnet root, 40 parts of pearl, 15 parts of bletilla, 25 parts of salvia miltiorrhiza, 25 parts of calcined calamine, 25 parts of dandelion, 15 parts of honeysuckle, 25 parts of cotton rose leaf, 25 parts of dragon's blood and 30 parts of ligusticum wallichii.
The invention provides a traditional Chinese medicine ointment for promoting wound healing, which is prepared from the external traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials.
The invention also provides a preparation method of the traditional Chinese medicine ointment for promoting wound healing, which comprises the following steps:
s1, mixing 10-40 parts of folium callicarpae formosanae, 20-40 parts of dalbergia wood, 15-30 parts of garden burnet root, 10-20 parts of rhizoma bletillae, 20-30 parts of salvia miltiorrhiza, 20-30 parts of dandelion, 10-20 parts of honeysuckle, 20-40 parts of ligusticum wallichii and 10-30 parts of cotton rose leaves, adding water for decoction, filtering, and concentrating the filtrate into clear paste to obtain a water extraction product;
s2, heating and refluxing the dregs obtained after water extraction in the step S1 with ethanol, filtering, and concentrating the filtrate under reduced pressure to obtain a clear paste to obtain an ethanol extraction product;
s3, mixing the water extraction product prepared in the step S1 with the alcohol extraction product prepared in the step S2, adding a proper amount of sesame oil, and steaming over water to obtain an oil-immersed ointment;
s4, respectively carrying out superfine grinding on 10-20 parts of pseudo-ginseng, 20-60 parts of pearl, 10-30 parts of calcined calamine and 10-30 parts of dragon's blood by using a traditional Chinese medicine superfine grinder to obtain nano powder of pseudo-ginseng, pearl, calcined calamine and dragon's blood;
s5, adding the nano-powder of the pseudo-ginseng, the pearl and the calcined calamine prepared in the step S4 into the oil-immersed ointment prepared in the step S3 while the ointment is hot, fully and uniformly mixing, and cooling to room temperature;
s6, dispersing the dragon' S blood nanopowder prepared in the step S4 with ethanol, adding into the medicine product cooled in the step S5, fully mixing uniformly, and canning to obtain the traditional Chinese medicine ointment for promoting wound healing.
Preferably, in the preparation of the oil-immersed ointment, the dosage of the sesame oil is 0.5 to 1.2 times of the volume sum of the water extraction product and the alcohol extraction product.
Preferably, the temperature of the invention is 50-80 ℃ in water-proof steaming, and the time is 30-40 minutes.
Preferably, in the preparation of the nano powder, the particle size of the nano powder is 100-300 nm.
Preferably, in the invention, in the process of dispersing the dragon blood, the dosage of the ethanol for dispersing the nanometer powder of the dragon blood is 3 to 5 times of the weight of the dragon blood.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention combines modern medical research with the theory of traditional Chinese medicine, and researches and screens various medicinal ingredients to obtain the external traditional Chinese medicine composition. Experiments show that the external traditional Chinese medicine composition has the effects of stopping bleeding, diminishing inflammation, relieving swelling and pain, removing stasis and promoting tissue regeneration, can effectively improve the blood circulation of local wound tissues, enhance the immunologic function and promote the growth of granulation tissues, thereby promoting the rapid healing of the wound; meanwhile, the transdermal absorption effect of the medicine is good, the effective rate of the medicine is obviously improved, and the dosage of the medicine is reduced.
2. The traditional Chinese medicine ointment disclosed by the invention is fine and smooth, has no rough feeling, can form a stable protective film on a wound surface, can promote division and regeneration of local histiocyte of the wound surface and promote quick healing of the wound surface, is mild and nonirritating in contact with skin, and does not increase pain during dressing change.
3. The preparation method of the traditional Chinese medicine ointment is simple and easy to implement, low in production cost, strong in operability and suitable for industrial production.
Drawings
FIG. 1 is a dynamic curve of the wound healing rate of rats in each group according to the embodiment of the present invention.
Detailed Description
In order to facilitate an understanding of the present invention, preferred embodiments of the present invention will be described below with reference to examples. It is to be understood that the following description is only intended to illustrate features and advantages of the present invention, and not to limit the claims of the present invention.
The present invention is not particularly limited in terms of the source of all raw materials, and may be commercially available.
The invention provides an external traditional Chinese medicine for promoting wound healing, which comprises the following raw material medicines in parts by weight: 10-20 parts of pseudo-ginseng, 20-40 parts of dalbergia wood, 10-40 parts of beautyberry leaf, 15-30 parts of garden burnet root, 20-60 parts of pearl, 10-20 parts of bletilla, 20-30 parts of salvia miltiorrhiza, 10-30 parts of calcined calamine, 20-30 parts of dandelion, 10-20 parts of honeysuckle, 10-30 parts of cotton rose leaf, 10-30 parts of dragon's blood and 20-40 parts of ligusticum wallichii.
The amount of the pseudo-ginseng is preferably 12 to 15 parts, and more preferably 15 parts. Notoginseng radix, with warm nature, has effects of removing blood stasis, stopping bleeding, relieving swelling, relieving pain, and resisting inflammation, can promote growth of various cell masses, and significantly improve phagocytosis rate and phagocytosis index of macrophage.
The amount of the rosewood is preferably 25 to 30 parts, and more preferably 30 parts. Lignum Dalbergiae Odoriferae, warm in nature, has effects of removing blood stasis, stopping bleeding, regulating qi-flowing, and relieving pain, and can promote the recovery of topical wound tissue microcirculation.
The using amount of the beautyberry leaves is preferably 15 to 30 parts, and more preferably 30 parts. Folium Callicarpae Formosanae, with cold nature, has effects of removing blood stasis, relieving swelling, astringing, stopping bleeding, diminishing inflammation, cooling blood, and relieving pain.
The garden burnet is preferably used in an amount of 20 to 25 parts, more preferably 25 parts. Sanguisorba officinalis is slightly cold in nature, has the effects of detoxifying and cooling blood, stopping bleeding and healing sore, inhibiting bacteria and resisting inflammation, and can promote wound healing.
The amount of the pearl is preferably 25 to 40 parts, and more preferably 40 parts. Pearl, cold in nature. Has effects of removing toxic substance, promoting granulation, caring skin, removing speckle, promoting collagen synthesis, and providing biological energy for wound healing.
The amount of the bletilla striata is preferably 12-15 parts, and more preferably 15 parts. Bletilla striata, being slightly cold in nature, has the functions of astringing to stop bleeding, detumescence and promoting granulation and antibiosis, and can maintain the blood volume of local wound tissues.
The amount of the salvia miltiorrhiza is preferably 22 to 25 parts, and more preferably 25 parts. The salvia miltiorrhiza is slightly cold in nature, has the effects of promoting blood circulation, removing blood stasis, cooling blood, relieving pain, resisting bacteria and diminishing inflammation, can accelerate microcirculation blood flow of local wound tissues, enhances microcirculation function and promotes wound healing.
The amount of calcined calamine is preferably 15 to 25 parts, more preferably 25 parts. Calcined calamine has mild property, has the effects of detoxifying, relieving itching, astringing dampness, healing sore, preventing corrosion and resisting bacteria, and can absorb wound secretion.
The amount of the dandelion is preferably 22 to 25 parts, more preferably 25 parts. The dandelion is cold in nature, has the effects of clearing heat and removing toxicity, reducing swelling and resolving masses and resisting bacteria, and the honeysuckle is preferably 12-15 parts, more preferably 15 parts. The honeysuckle is cold in nature, has the functions of clearing away heat and toxic materials, dispelling wind and heat, inhibiting bacteria and resisting inflammation, and can enhance the immune function of local wound tissues.
The preferable dosage of the folium Hibisci Mutabilis is 15-25 parts, and more preferably 25 parts. The folium Hibisci Mutabilis has mild nature, and has effects of removing toxic substance, cooling blood, relieving swelling and pain, and accelerating discharge of wound secretion.
The dosage of the dragon blood is preferably 15-25 parts, and more preferably 25 parts. Dragon's blood, neutral in nature, has the functions of promoting blood circulation to arrest pain, dissipating blood stasis to stop bleeding, promoting granulation, healing wound and resisting bacteria.
The amount of the ligusticum wallichii is preferably 25-30 parts, and more preferably 30 parts. The ligusticum wallichii is warm in nature, has the effects of promoting blood circulation, promoting qi circulation, dispelling wind, relieving pain and resisting bacteria, can improve the microcirculation of local wound tissues, enhances the metabolic function of cells, and simultaneously promotes skin penetration and absorption.
According to the traditional Chinese medicine, the injury causes local qi and blood stasis, meridian obstruction and internal pathogenic toxin. Stagnant blood, new blood, swelling and toxin are not generated, and the wound surface is difficult to heal. Therefore, its therapeutic principle is to remove blood stasis and promote granulation, relieve swelling and pain, activate blood and cure toxicity. Modern medicine considers that blood vessels are damaged after trauma, local cell tissue functions are reduced, further inflammatory reaction is triggered, and the wound surface is difficult to heal.
The invention combines the traditional Chinese medicine theory and the modern medical research to obtain the external traditional Chinese medicine composition for promoting the healing of the wound surface. In the formula of the traditional Chinese medicine composition, the pseudo-ginseng, the rosewood heart wood and the callicarpa bodinieri leaf are monarch drugs and are used for stopping bleeding, dissipating blood stasis, relieving swelling and pain, promoting the recovery of the microcirculation of local wound tissues and enhancing the functions of local tissue cells; the sanguisorba, the bletilla, the salvia miltiorrhiza, the dragon's blood and the pearl are used as ministerial medicines, have the functions of promoting blood circulation, removing blood stasis, astringing, stopping bleeding, reducing swelling and promoting granulation, and the pseudo-ginseng, the dalbergia wood and the folium callicarpae formosanae are used for stopping bleeding, removing blood stasis, promoting blood circulation, reducing swelling, relieving pain, promoting granulation and effectively improving microcirculation of local wound tissues; calcined calamine, dandelion, honeysuckle and cotton rose hibiscus leaves are adjuvant drugs, and the traditional Chinese medicine composition has the effects of clearing away heat and toxic materials, eliminating dampness, relieving itching, resisting bacteria, diminishing inflammation, promoting absorption and leakage of wound secretion and relieving inflammatory reaction; rhizoma Ligustici Chuanxiong is used as a guiding drug to dispel pathogenic wind and promote qi circulation. The medicines are matched to play the effects of stopping bleeding, removing blood stasis, relieving swelling and pain, diminishing inflammation and promoting tissue regeneration, and have the obvious effect of promoting wound healing when being applied to wound healing.
In some preferred embodiments of the present invention, the external traditional Chinese medicine composition for promoting wound healing comprises the following raw material medicines: 15 parts of pseudo-ginseng, 30 parts of dalbergia wood, 30 parts of beautyberry leaf, 25 parts of garden burnet root, 40 parts of pearl, 15 parts of bletilla, 25 parts of salvia miltiorrhiza, 25 parts of calcined calamine, 25 parts of dandelion, 15 parts of honeysuckle, 25 parts of cotton rose leaf, 25 parts of dragon's blood and 30 parts of ligusticum wallichii.
In some preferred embodiments of the present invention, the external traditional Chinese medicine composition for promoting wound healing comprises the following raw material medicines: 12 parts of pseudo-ginseng, 25 parts of dalbergia wood, 15 parts of beautyberry leaf, 20 parts of garden burnet root, 25 parts of pearl, 12 parts of bletilla, 22 parts of salvia miltiorrhiza, 15 parts of calcined calamine, 22 parts of dandelion, 12 parts of honeysuckle, 15 parts of cotton rose leaf, 15 parts of dragon's blood and 25 parts of ligusticum wallichii.
In some preferred embodiments of the present invention, the external traditional Chinese medicine composition for promoting wound healing comprises the following raw material medicines: 10 parts of pseudo-ginseng, 20 parts of dalbergia wood, 10 parts of folium callicarpae pedunculalae, 15 parts of garden burnet root, 20 parts of pearl, 10 parts of bletilla, 20 parts of salvia miltiorrhiza, 10 parts of calcined calamine, 20 parts of dandelion, 10 parts of honeysuckle, 10 parts of cotton rose hibiscus leaf, 10 parts of dragon's blood and 20 parts of ligusticum wallichii.
In some preferred embodiments of the present invention, the external traditional Chinese medicine composition for promoting wound healing comprises the following raw material medicines: 20 parts of pseudo-ginseng, 40 parts of dalbergia wood, 40 parts of beautyberry leaf, 30 parts of garden burnet root, 60 parts of pearl, 20 parts of bletilla, 30 parts of salvia miltiorrhiza, 30 parts of calcined calamine, 30 parts of dandelion, 20 parts of honeysuckle, 30 parts of cotton rose leaf, 30 parts of dragon's blood and 40 parts of ligusticum wallichii.
The invention provides a traditional Chinese medicine ointment for promoting wound healing, which is prepared from the external traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials.
The invention also provides a preparation method of the traditional Chinese medicine ointment for promoting wound healing, which comprises the following steps:
s1, mixing 10-40 parts of folium callicarpae formosanae, 20-40 parts of dalbergia wood, 15-30 parts of garden burnet root, 10-20 parts of rhizoma bletillae, 20-30 parts of salvia miltiorrhiza, 20-30 parts of dandelion, 10-20 parts of honeysuckle, 20-40 parts of ligusticum wallichii and 10-30 parts of cotton rose leaves, adding water for decoction, filtering, and concentrating the filtrate into clear paste to obtain a water extraction product;
s2, heating and refluxing the dregs obtained after water extraction in the step S1 with ethanol, filtering, and concentrating the filtrate under reduced pressure to obtain a clear paste to obtain an ethanol extraction product;
s3, mixing the water extraction product prepared in the step S1 with the alcohol extraction product prepared in the step S2, adding a proper amount of sesame oil, and steaming over water to obtain an oil-immersed ointment;
s4, respectively carrying out superfine grinding on 10-20 parts of pseudo-ginseng, 20-60 parts of pearl, 10-30 parts of calcined calamine and 10-30 parts of dragon's blood by using a traditional Chinese medicine superfine grinder to obtain nano powder of pseudo-ginseng, pearl, calcined calamine and dragon's blood;
s5, adding the nano-powder of the pseudo-ginseng, the pearl and the calcined calamine prepared in the step S4 into the oil-immersed ointment prepared in the step S3 while the ointment is hot, fully and uniformly mixing, and cooling to room temperature;
s6, dispersing the dragon' S blood nanopowder prepared in the step S4 with ethanol, adding into the medicine product cooled in the step S5, fully mixing uniformly, and canning to obtain the traditional Chinese medicine ointment for promoting wound healing.
In the preparation of the oil-immersed ointment, the dosage of the sesame oil is 0.5 to 1.2 times of the volume sum of the water extraction product and the alcohol extraction product.
The invention is used in water-proof steaming at 50-80 deg.C for 30-40 min.
In the preparation of the nano powder, the particle size of the nano powder is 100-300 nm.
In the invention, in the process of dispersing the dragon blood, the dosage of the ethanol for dispersing the nanometer powder of the dragon blood is 3 to 5 times of the weight of the dragon blood.
In order to further explain the present invention, the following will describe in detail an ointment of an external traditional Chinese medicine composition for promoting wound healing and a preparation method thereof, which are provided by the present invention, with reference to examples.
Example 1-preparation of an external traditional Chinese medicine ointment for promoting wound healing according to the present invention:
s1, weighing 30g of dalbergia wood, 30g of folium callicarpae formosanae, 25g of dried sanguisorba officinalis, 15g of rhizoma bletillae, 25g of salvia miltiorrhiza, 25g of dandelion, 15g of honeysuckle, 25g of cotton rose hibiscus leaves and 30g of ligusticum wallichii, mixing, adding 8-10 times of deionized water, decocting and extracting for 2 times, each time for 1.5 hours, filtering, concentrating the filtrate into clear paste with the relative density of 1.10 (room temperature), and obtaining a water extraction product;
s2, adding 75% ethanol with the volume of 8-10 times that of the decoction dregs obtained after the water extraction in the step S1, heating and refluxing for 2 times, wherein each time lasts for 1.5h, filtering, and concentrating the filtrate under reduced pressure into clear paste with the relative density of 1.10 (room temperature) to obtain an ethanol extraction product;
s3, putting the water extraction product prepared in the step S1 and the alcohol extraction product prepared in the step S2 into a container, mixing, adding sesame oil with the volume 0.5-1.2 times that of the mixed extraction product, putting the container into a constant-temperature water tank, steaming in a water-proof manner at 50-80 ℃ for 30-40min to obtain an oil-immersed ointment, and preserving at the constant temperature of 35 ℃;
s4, weighing 15g of pseudo-ginseng, 40g of pearl, 25g of calcined calamine and 25g of dragon blood, and respectively carrying out superfine grinding by using a traditional Chinese medicine superfine grinder (commercially available) to obtain nano powder of pseudo-ginseng, pearl, calcined calamine and dragon blood;
s5, adding the nano-powder of the pseudo-ginseng, the pearl and the calcined calamine prepared in the step S4 into the oil-immersed ointment prepared in the step S3 while the ointment is hot, fully and uniformly mixing, and cooling to room temperature;
s6, dispersing the dragon ' S blood nano powder prepared in the step S4 by using 75% ethanol with the volume 5-7 times that of the dragon ' S blood nano powder, adding the dispersed dragon ' S blood nano powder into the product cooled in the step S5, fully and uniformly mixing, and canning to obtain the traditional Chinese medicine ointment for promoting wound healing.
Example 2-preparation of an external traditional Chinese medicine ointment for promoting wound healing according to the present invention:
s1, weighing 25g of dalbergia wood, 15g of folium callicarpae formosanae, 20g of dried sanguisorba officinalis, 12g of rhizoma bletillae, 22g of salvia miltiorrhiza, 22g of dandelion, 12g of honeysuckle, 15g of cotton rose hibiscus leaves and 25g of ligusticum wallichii, mixing, adding 8-10 times of deionized water, decocting and extracting for 2 times, each time for 1.5 hours, filtering, concentrating the filtrate into clear paste with the relative density of 1.10 (room temperature), and obtaining a water extraction product;
s2, adding 75% ethanol with the volume of 8-10 times that of the decoction dregs obtained after the water extraction in the step S1, heating and refluxing for 2 times, wherein each time lasts for 1.5h, filtering, and concentrating the filtrate under reduced pressure into clear paste with the relative density of 1.10 (room temperature) to obtain an ethanol extraction product;
s3, putting the water extraction product prepared in the step S1 and the alcohol extraction product prepared in the step S2 into a container, mixing, adding sesame oil with the volume 0.5-1.2 times that of the mixed extraction product, putting the container into a constant-temperature water tank, steaming in a water-proof manner at 50-80 ℃ for 30-40min to obtain an oil-immersed ointment, and preserving at the constant temperature of 35 ℃;
s4, weighing 12g of pseudo-ginseng, 25g of pearl, 15g of calcined calamine and 15g of dragon blood, and respectively carrying out superfine grinding by using a traditional Chinese medicine superfine grinder (commercially available) to obtain nano powder of pseudo-ginseng, pearl, calcined calamine and dragon blood;
s5, adding the nano-powder of the pseudo-ginseng, the pearl and the calcined calamine prepared in the step S4 into the oil-immersed ointment prepared in the step S3 while the ointment is hot, fully and uniformly mixing, and cooling to room temperature;
s6, dispersing the dragon ' S blood nano powder prepared in the step S4 by using 75% ethanol with the volume 5-7 times that of the dragon ' S blood nano powder, adding the dispersed dragon ' S blood nano powder into the product cooled in the step S5, fully and uniformly mixing, and canning to obtain the traditional Chinese medicine ointment for promoting wound healing.
Example 3-preparation of an external traditional Chinese medicine ointment for promoting wound healing according to the present invention:
s1, weighing 20g of dalbergia wood, 10g of folium callicarpae formosanae, 15g of dried sanguisorba officinalis, 10g of rhizoma bletillae, 20g of salvia miltiorrhiza, 20g of dandelion, 10g of honeysuckle, 10g of cotton rose hibiscus leaves and 20g of ligusticum wallichii, mixing, adding 8-10 times of deionized water, decocting and extracting for 2 times, each time for 1.5 hours, filtering, concentrating the filtrate into clear paste with the relative density of 1.10 (room temperature), and obtaining a water extraction product;
s2, adding 75% ethanol with the volume of 8-10 times that of the decoction dregs obtained after the water extraction in the step S1, heating and refluxing for 2 times, wherein each time lasts for 1.5h, filtering, and concentrating the filtrate under reduced pressure into clear paste with the relative density of 1.10 (room temperature) to obtain an ethanol extraction product;
s3, putting the water extraction product prepared in the step S1 and the alcohol extraction product prepared in the step S2 into a container, mixing, adding sesame oil with the volume 0.5-1.2 times that of the mixed extraction product, putting the container into a constant-temperature water tank, steaming in a water-proof manner at 50-80 ℃ for 30-40min to obtain an oil-immersed ointment, and preserving at the constant temperature of 35 ℃;
s4, weighing 10g of pseudo-ginseng, 20g of pearl, 10g of calcined calamine and 10g of dragon's blood, and respectively carrying out superfine grinding by using a traditional Chinese medicine superfine grinder (commercially available) to obtain nano powder of pseudo-ginseng, pearl, calcined calamine and dragon's blood;
s5, adding the nano-powder of the pseudo-ginseng, the pearl and the calcined calamine prepared in the step S4 into the oil-immersed ointment prepared in the step S3 while the ointment is hot, fully and uniformly mixing, and cooling to room temperature;
s6, dispersing the dragon ' S blood nano powder prepared in the step S4 by using 75% ethanol with the volume 5-7 times that of the dragon ' S blood nano powder, adding the dispersed dragon ' S blood nano powder into the product cooled in the step S5, fully and uniformly mixing, and canning to obtain the traditional Chinese medicine ointment for promoting wound healing.
Example 4-preparation of an external traditional Chinese medicine ointment for promoting wound healing according to the present invention:
s1, weighing 40g of dalbergia wood, 40g of folium callicarpae formosanae, 30g of dried sanguisorba officinalis, 20g of rhizoma bletillae, 30g of salvia miltiorrhiza, 30g of dandelion, 20g of honeysuckle, 30g of cotton rose hibiscus leaves and 40g of ligusticum wallichii, mixing, adding 8-10 times of deionized water, decocting and extracting for 2 times, each time for 1.5 hours, filtering, concentrating the filtrate into clear paste with the relative density of 1.10 (room temperature), and obtaining a water extraction product;
s2, adding 75% ethanol with the volume of 8-10 times that of the decoction dregs obtained after the water extraction in the step S1, heating and refluxing for 2 times, wherein each time lasts for 1.5h, filtering, and concentrating the filtrate under reduced pressure into clear paste with the relative density of 1.10 (room temperature) to obtain an ethanol extraction product;
s3, putting the water extraction product prepared in the step S1 and the alcohol extraction product prepared in the step S2 into a container, mixing, adding sesame oil with the volume 0.5-1.2 times that of the mixed extraction product, putting the container into a constant-temperature water tank, steaming in a water-proof manner at 50-80 ℃ for 30-40min to obtain an oil-immersed ointment, and preserving at the constant temperature of 35 ℃;
s4, weighing 20g of pseudo-ginseng, 60g of pearl, 30g of calcined calamine and 30g of dragon blood, and respectively carrying out superfine grinding by using a traditional Chinese medicine superfine grinder (commercially available) to obtain nano powder of the pseudo-ginseng, the pearl, the calcined calamine and the dragon blood;
s5, adding the nano-powder of the pseudo-ginseng, the pearl and the calcined calamine prepared in the step S4 into the oil-immersed ointment prepared in the step S3 while the ointment is hot, fully and uniformly mixing, and cooling to room temperature;
s6, dispersing the dragon ' S blood nano powder prepared in the step S4 by using 75% ethanol with the volume 5-7 times that of the dragon ' S blood nano powder, adding the dispersed dragon ' S blood nano powder into the product cooled in the step S5, fully and uniformly mixing, and canning to obtain the traditional Chinese medicine ointment for promoting wound healing.
Example 5-effect test of the external traditional Chinese medicine ointment for promoting wound healing of the invention:
1. animal testing
1.1 test for promoting wound healing
1.1.1 topical Chinese medicinal ointment
Externally applied Chinese medicinal ointments prepared in examples 1, 2, 3 and 4, respectively;
wound treatment agent: 30g of folium callicarpae pedunculalae, 15g of dried sanguisorba officinalis, 6g of rosewood heart wood, 12g of dried corydalis bungeana, 3g of myrrh, 6g of angelica, 12g of salvia chinensis, 15g of prepared rehmannia root, 12g of lithospermum and 20g of liquorice are taken, deionized water with the volume of 8-10 times is added, the decoction and the extraction are carried out, the filtration is carried out, and the filtrate is concentrated into an extract with the relative density of 1.10 (room temperature).
1.1.2 grouping and administration of test animals
Wistar rats (200 g, 20 g) 60 were randomly divided into six groups of 10 rats each, four experimental groups (A, B, C, D), a control group (Y) and a blank group (K). After anaesthesia with ether, 10% Na was applied to both dorsal sides2S, depilating, namely, using a puncher to make circular defect wounds till the deep layer of the fascia has the diameter of 2 cm. After the model building is successful, the rat is raised in a single cage, and four experimental groups respectively daub the external traditional Chinese medicine paste prepared in the embodiment 1-4 on the wound of the model building rat once a day, and the thickness of the paste is about 1 mm; applying the wound treatment agent to the wound of the control group once a day, wherein the wound is about 1mm thick; the control group was sterilized daily and given 2% iodine tincture.
1.1.3 Experimental observations
After the application, the wound healing rate, the color of the wound surface, the amount of exudate of the wound surface and the growth condition of granulation tissue are respectively observed at 1d, 3d, 7d and 14d and recorded.
1.1.4 test results
1.1.4.1 toxicity study
During the experiment, all rats have good general conditions, no adverse reactions such as diarrhea, food refusal and the like, no obvious toxic or side effect and no death of the rats, which shows that the external traditional Chinese medicine ointment is safe and nontoxic.
1.1.4.2 Effect on wound healing
All wounds had bleeding, exudation and edema of surrounding tissues after molding. Blood and exudate rapidly coagulate to form blood clots that fill the wound bed. After 3d, most of the wound surfaces of each experimental group and the control group are still covered by blood clots, but partial granular granulation can be seen at the wound edge, the edema of tissues around the wound surface is reduced to a certain extent, the wound surface is moist, and no obvious difference exists between each experimental group and the control group; the blank group of wound surfaces are almost completely covered by blood clots, granular granulation is few, edema of tissues around the wound surfaces is still obvious, and the wound surfaces are dry. After 7d, the wound surfaces of all groups are wet, secretion is more, 60.5% of wound surfaces are filled with granulation tissues, the wound surfaces are bright red, part of thin-layer epithelium can be seen at the edges of the wound surfaces to grow, the area of the wound surfaces is reduced by 51.4%, and no obvious difference exists among all groups; the wound surface of the control group is wet, the secretion is more, 52.0 percent of the wound surface is filled with granulation tissue, the wound surface is bright red, the thin epithelial layer at the edge of the wound surface can grow, and the area of the wound surface is reduced by 42.7 percent; the blank group had dry wound surface and less secretion, and only 38.6% of the wound surface was filled with granulation tissue and had scab phenomenon. After 14 days, each group is almost completely filled with granulation tissues and has epithelial growth, more hair can grow out, the wound surface is basically healed, only a small scar area is left, no hair grows out, and no obvious difference exists among the groups; 92.8% of wounds in the control group were filled with granulation tissue, 89.5% of wounds in the blank group were filled with granulation tissue, and fewer epithelia and hair were grown.
The healing rate of the wound surface of each group was statistically processed, and the results are shown in table 1.
TABLE 1 dynamic changes in the rate of wound healing in rats of various groups
Figure DEST_PATH_IMAGE001
As can be seen from Table 1, the difference between the healing rate of each experimental group and that of the control group at 3d is not significant, but is obvious compared with that of the blank group; the healing rate difference between the experimental groups is not significant at 7d and 14d, but is more significant compared with the control group, and the healing rate of the experimental group and the control group is far higher than that of the blank group.
Dynamic curves were drawn according to the healing rates of the wounds of the rats in each group in table 1, as shown in fig. 1.
1.2 in vitro bacteriostatic test
1.2.1 test methods
The external use Chinese medicinal ointments prepared in examples 1 to 4 were uniformly applied to circular filter paper sheets having a diameter of 1.0cm, respectively, and the paper sheets were attached after the water on the plates was completely absorbed by agar. The drug sensitive paper is taken by a sterile forceps and is pasted on the surface of the flat plate, and the paper can not be taken up once the paper is pasted. And 3 paper sheets are pasted on each flat plate, the distance between every two paper sheets is not less than 24mm, the distance between the center of each paper sheet and the edge of the flat dish is not less than 15mm, and the paper sheets are pasted within 15min after the bacteria inoculation. All manipulations were performed under sterile conditions. Incubating at 37 deg.C for 18-24 h. The antibacterial effect of the drug was evaluated by the diameter (cm) of the zone of inhibition.
1.2.2 evaluation of Effect
The diameter of the inhibition zone is more than 1.15cm, which is indicated by +++ for strong inhibition; the diameter of the inhibition zone is 1.05-1.15cm, which is expressed by + + for inhibition; weak bacteriostasis is achieved when the diameter of the bacteriostasis zone is less than 1.05cm, and is expressed as plus; the diameter of the inhibition zone of 1.00cm is used as the expression of no inhibition.
1.2.3 test results
TABLE 2 in vitro bacteriostatic effect of the topical Chinese medicinal paste of the present invention
Figure DEST_PATH_IMAGE002
As can be seen from table 2, the external traditional Chinese medicine ointment prepared in each example has a better in vitro antibacterial effect, and the difference between the in vitro antibacterial effects of each example is not significant.
2. Clinical research
2.1 patient data and grouping
Totally observing 60 patients without lower limb wound infection, dividing into treatment group and control group at random, treating the treatment group with the topical Chinese medicinal ointment prepared in example 1 after the two groups are treated with medical alcohol, treating the control group with wound treating agent, and treating the wound area with 140.5 + -4.50 cm2There is comparability between cases.
Wound healing was observed before each application and treatment continued for 14 days.
2.2 Observation of wound healing Rate
After the observation, the shape and size of the wound surface are drawn by transparent grid tissue paper, the pattern drawn by a camera is used for photographing, the picture is led into a computer, the area of the wound surface is calculated by ImageJ software, and the condensation rate is calculated.
The healing rate is calculated when the condensation rate reaches more than 98%.
Condensation rate = (original area of wound surface-area covered by wound surface)/original area of wound surface x 100%.
2.3 therapeutic results
TABLE 3-14d healing rates for treatment
Figure DEST_PATH_IMAGE003
As can be seen from Table 3, the experimental group has a better healing rate than the control group after 14d treatment, which indicates that the external traditional Chinese medicine ointment can promote the wound to heal more quickly.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.

Claims (9)

1. The external traditional Chinese medicine composition for promoting wound healing is characterized by being prepared from the following components in parts by weight: 10-20 parts of pseudo-ginseng, 20-40 parts of dalbergia wood, 10-40 parts of beautyberry leaf, 15-30 parts of garden burnet root, 20-60 parts of pearl, 10-20 parts of bletilla, 20-30 parts of salvia miltiorrhiza, 10-30 parts of calcined calamine, 20-30 parts of dandelion, 10-20 parts of honeysuckle, 10-30 parts of cotton rose leaf, 10-30 parts of dragon's blood and 20-40 parts of ligusticum wallichii.
2. The topical Chinese medicinal composition for promoting wound healing according to claim 1, wherein the composition is prepared from the following components: 12-15 parts of pseudo-ginseng, 25-30 parts of dalbergia wood, 15-30 parts of beautyberry leaf, 20-25 parts of garden burnet root, 25-40 parts of pearl, 12-15 parts of bletilla, 22-25 parts of salvia miltiorrhiza, 15-25 parts of calcined calamine, 22-25 parts of dandelion, 12-15 parts of honeysuckle, 15-25 parts of cotton rose leaf, 15-25 parts of dragon's blood and 25-30 parts of ligusticum wallichii.
3. The topical Chinese medicinal composition for promoting wound healing according to claim 1, wherein the composition is prepared from the following components: 15 parts of pseudo-ginseng, 30 parts of dalbergia wood, 30 parts of beautyberry leaf, 25 parts of garden burnet root, 40 parts of pearl, 15 parts of bletilla, 25 parts of salvia miltiorrhiza, 25 parts of calcined calamine, 25 parts of dandelion, 15 parts of honeysuckle, 25 parts of cotton rose leaf, 25 parts of dragon's blood and 30 parts of ligusticum wallichii.
4. A Chinese medicinal ointment for promoting wound healing, which is prepared from the external Chinese medicinal composition of any one of claims 1 to 3 and pharmaceutically acceptable adjuvants.
5. The method for preparing a Chinese medicinal ointment for promoting wound healing as claimed in claim 4, which comprises the steps of:
s1, mixing folium callicarpae pedunculalae, dalbergia wood, garden burnet root, bletilla, salvia miltiorrhiza, dandelion, honeysuckle, ligusticum wallichii and cotton rose hibiscus leaves, adding water for decoction, filtering, and concentrating the filtrate into clear paste to obtain a water extraction product;
s2, heating and refluxing the dregs after water extraction by using ethanol, filtering, and concentrating the filtrate under reduced pressure to obtain a clear paste to obtain an ethanol extraction product;
s3, mixing the water extraction product and the alcohol extraction product, adding sesame oil, and steaming in a water-proof manner to obtain an oil-immersed ointment;
s4, carrying out superfine grinding on the pseudo-ginseng, the pearl, the calcined calamine and the dragon blood to obtain nano powder of the pseudo-ginseng, the pearl, the calcined calamine and the dragon blood;
s5, adding the nano-powder of the pseudo-ginseng, the pearl and the calcined calamine into the oil-immersed ointment while the oil-immersed ointment is hot, fully mixing the materials uniformly, and cooling the mixture;
s6, dispersing the nano powder of the dragon 'S blood with ethanol, adding the dispersed nano powder into the product cooled in the step S5, fully and uniformly mixing, and canning to obtain the dragon' S blood.
6. The method for preparing a Chinese medicinal ointment for promoting wound healing according to claim 5, wherein the amount of the sesame oil is 0.5 to 1.2 times of the sum of the volumes of the water extract and the alcohol extract.
7. The method for preparing a Chinese medicinal ointment for promoting wound healing according to claim 5, wherein the steaming is performed at 50-80 deg.C for 30-40 min.
8. The method for preparing a Chinese medicinal ointment for promoting wound healing as claimed in claim 5, wherein the particle size of the nanopowder is 100-300 nm.
9. The method for preparing a Chinese medicinal ointment for promoting wound healing according to claim 5, wherein the amount of ethanol used for dispersing the sanguis Draxonis nanopowder is 3-5 times of the weight of sanguis Draxonis.
CN202010463029.9A 2020-05-27 2020-05-27 A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method Expired - Fee Related CN111467433B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010463029.9A CN111467433B (en) 2020-05-27 2020-05-27 A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010463029.9A CN111467433B (en) 2020-05-27 2020-05-27 A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method

Publications (2)

Publication Number Publication Date
CN111467433A CN111467433A (en) 2020-07-31
CN111467433B true CN111467433B (en) 2021-08-20

Family

ID=71764886

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010463029.9A Expired - Fee Related CN111467433B (en) 2020-05-27 2020-05-27 A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method

Country Status (1)

Country Link
CN (1) CN111467433B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113230409A (en) * 2020-12-28 2021-08-10 上海奥茵特生物科技有限公司 Traditional Chinese medicine composite matrix ointment for promoting wound healing
CN112704691B (en) * 2021-02-25 2022-07-08 山东中医药大学 Traditional Chinese medicine ointment for promoting wound healing and reducing scar formation and preparation method thereof
CN114796596A (en) * 2022-04-22 2022-07-29 青岛大学 Nanofiber hydrogel dressing loaded with salvia miltiorrhiza and radix puerariae and preparation process thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101406555A (en) * 2007-10-08 2009-04-15 韩欣红 Powder for healing burn
CN101347559A (en) * 2008-07-02 2009-01-21 刘彦宾 Medicament for treating ulcer of skin
CN102813751B (en) * 2012-08-09 2014-04-09 张迎春 Wound treatment agent

Also Published As

Publication number Publication date
CN111467433A (en) 2020-07-31

Similar Documents

Publication Publication Date Title
CN111467433B (en) A topical Chinese medicinal composition and paste for promoting wound healing, and its preparation method
CN102091203B (en) External traditional Chinese medicine preparation for treating chronic wounds and preparation method thereof
CN102755446A (en) Tea oil burn cream and preparation method thereof
CN101164574B (en) Medicine for treating burns and scalds and its preparation method
CN102266406B (en) Chinese medicinal ointment for treating burn and preparation method
CN104225258B (en) A kind of Chinese medicine composition promoting wound healing
CN106581439A (en) Traditional Chinese medicine composition for treating wounds, diabetic feet and skin ulceration, and preparation method thereof
CN105770116A (en) Medicine composition for treating burn and scald, method for preparing medicine composition and application thereof
WO2012100754A1 (en) Chinese medicine composition for treating stab wounds, burns, and various traumas
CN109453414A (en) A kind of postoperative anti-infectious dressing of liver and gall surgical department
CN107982387A (en) A kind of Chinese medicine composition for treating scald and preparation method thereof
CN108096506A (en) One kind is dispelled wart Chinese medicinal plant extracting solution and preparation method thereof
CN103977367A (en) Traditional Chinese medicament for treating qi-stagnation blood-aggregation type bedsore and preparation method thereof
CN1176675C (en) Burn treating medicine and its prepn
CN111544514A (en) A Chinese medicinal ointment for treating burn and scald, and its preparation method
CN111658711A (en) Traditional Chinese medicine formula for treating burns and scalds
CN104721444A (en) Chinese medicinal compositions for treating burns and preparation methods thereof
CN101658583B (en) Traditional Chinese medicine for treating cold-dampness type skin ulcer and preparation method thereof
CN108403910A (en) A kind of tea medicine for treating eczema
CN111700989B (en) Traditional Chinese medicine composition for dispelling wind, eliminating dampness and treating tinea pedis and preparation method thereof
WO2018090848A1 (en) Chinese medicine granule for invigorating spleen, removing obstruction in collaterals and detoxification
CN107050298A (en) A kind of Chinese medicine paste formulation and preparation method for treating burn and scald skin damaged
CN106692746A (en) Externally used energy oil for improving hypomenorrhea and light menses, and preparation method thereof
CN106236900A (en) A kind of for compound medicine treating burn and preparation method thereof
CN105362675A (en) Emulsifiable paste for treating plasmacellular mastitis and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20210820

CF01 Termination of patent right due to non-payment of annual fee