CN109511535A - A kind of dendrobium candidum bionic cultivation method - Google Patents

A kind of dendrobium candidum bionic cultivation method Download PDF

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Publication number
CN109511535A
CN109511535A CN201811590710.9A CN201811590710A CN109511535A CN 109511535 A CN109511535 A CN 109511535A CN 201811590710 A CN201811590710 A CN 201811590710A CN 109511535 A CN109511535 A CN 109511535A
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dendrobium candidum
dendrobium
parts
hardening
cultivation method
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CN109511535B (en
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张尚文
张向军
李婷
黄诗宇
庾韦花
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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Biotechnology Research Institute Guangxi Academy Of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to dendrobium candidum planting technology fields, in particular to a kind of dendrobium candidum bionic cultivation method, the application is in the cultivation of dendrobium candidum, it is preferred that dendrobium nobile seedling, and the hardening treatment of specificity is carried out, the germination rate of base portion can be effectively improved, and the growth length of new rudiment is not less than old item, with short production cycle, effect is good;The non-woven fabrics of the application is degradation material, in the picking in 1 year of upper tree, do not need to wipe out again binding material process can save manually, time cost;In hardening, China fir is processed into and retains wooden batten, be easy to bundle tree, saved artificial;In dendrobium candidum binding after the application hardening when setting, matrix is smeared in wooden face, can adhere to dendrobium nobile base portion after hardening, have that adhesive force is strong, is difficult to be melted by rainwater, grown for the attachment of dendrobium nobile root and dendrobium nobile and provide the effect of nutrition;To improve adaptability, growing way, survival rate and the polyoses content of the bionical plantation of dendrobium candidum.

Description

A kind of dendrobium candidum bionic cultivation method
[technical field]
The present invention relates to dendrobium candidum planting technology field, in particular to a kind of dendrobium candidum bionic cultivation method.
[background technique]
Dendrobium candidum (Dendrobium officinaleKimura et Migo), is born in height above sea level up to 1600 meters of mountainous region On half dark and damp rock.It is distributed mainly on the ground such as Chinese Anhui, Zhejiang, Fujian.Its stem is used as medicine, and belongs to the yin tonics in tonic: Reinforcing stomach reg fluid, nourishing Yin and clearing heat belong to traditional rare Chinese medicine, and diseases a variety of for tumour etc. just have good effect.Due to pole High nutritive value and be known as " mesona ".Dendrobium polysaccharide is one of principle active component in dendrobium candidum.Dendrobium polysaccharide content Height be dendrobium candidum quality quality main judge index.Currently, dendrobium candidum main organized training in the course of cultivation Support, nursery culture the methods of, however, the dendrobium candidum of these traditional cultivation methods or because agriculture it is residual exceeded or Do not favored by market because polyoses content is low;Dendrobium officinale is because of its polyoses content height, and effect is good, by extinctive excavation. Therefore, how many medicinal herb grower's researchs at present carry out the bionical plantation of dendrobium candidum.Due in bionical planting process, because opening Environment is put, various pest and disease damages occur seriously, and environment is uncontrollable, and dendrobium candidum pseudo-wild cultivating always exists low output, is produced into This height, the factors such as dendrobium polysaccharide content is not high, risk is uncontrollable, it is difficult to be widely applied.
[summary of the invention]
In view of above content, it is necessary to provide a kind of high and low cost of adaptability, yield height, high survival rate, dendrobium polysaccharide and contain Amount height, the good officinal dendrobium stem plantation method easy to spread of quality.
In order to achieve the above objectives, the technical scheme adopted by the invention is that:
A kind of dendrobium candidum bionic cultivation method, described method includes following steps:
(1) hardening: selection bed plant the time be greater than 2 years, fresh length greater than 20cm dendrobium nobile from seedling be dendrobium nobile seedling;By China fir Leftover pieces after wood processing are cut into 5cm wide, the China fir item of 40-50cm, retain on one side wooden, are on one side bark face;Use nonwoven Dendrobium nobile seedling is fixed on the bark face of China fir item by cloth, retains dendrobium nobile seedling root;Dendrobium candidum quantity is bundled on the China fir item Not less than 3 clumps;To having bundled the watering of the China fir item after dendrobium nobile seedling, the 1-2 days, watering 1-2 time daily, the 3-5 days, daily watering Once, primary every watering in 2-3 days after the 7th day;It is grown to new root, terminates hardening;
(2) attached base: retaining wooden face to smear a layer thickness to the China fir item after step (1) hardening is 0.5-1cm's Matrix obtains Growth of Dendrobium candidum matrix after matrix hardening;
(3) growing substrate of step (2) bionical plantation: is bundled into the corresponding site for the tree living that grows nonparasitically upon another plant with non-woven fabrics;To attached Raw tree is trimmed, and guarantees that the closing of its leaf are 40%-70%;During plantation, every month is to dendrobium candidum foliage spray leaf Leaven.
Further, the tree that grows nonparasitically upon another plant of the step (3) is Qinggang wood, pine tree or Chinese chestnut tree.
Further, the matrix of the step (2) comprises the following components in parts by weight: 12 parts -18 parts of clay, 21 - 27 parts of fermentation sheep dung of part, 7 parts -17 parts of starch, 5 parts -15 parts of quick lime, 10 parts -17 parts of plant enzyme composition.
Further, the foliar fertilizer of the step (3) is made by plant enzyme and water according to mass ratio for 1:200-300.
Further, the starch is one of glutinous rice starch, flour, tapioca and/or potato starch or several Kind combination.
Further, the plant enzyme by probiotics, dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple, cedar sawdust, Sodium selenite, amino acid and sodium alkyl benzene sulfonate are made.
Further, the dendrobium candidum leaf, dendrobium candidum root, pueraria lobata, pineapple are Fresh Plants.
Further, the plant enzyme the preparation method comprises the following steps:
(1) strain expands and prepares: brown sugar being mixed with wheat bran according to weight ratio for 1:8-12, the benefit of 1mL-3mL is accessed Raw bacterium adds the warm water with mixture equal volume, ferments 24 hours, is obtained after expanding culture at being 25-29 DEG C in temperature Strain;
(2) it is 3-4:5-6:1-3 according to mass ratio by dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple and cedar sawdust: 2-5:1-3 mixing, it is that 1:8-14 is inoculated with that step (1), which is then expanded the strain after culture and mixture according to mass ratio, Be sealed by fermentation: it is stand-by to filter to take supernatant by 90-95d;
(3) sodium selenite solution for being 100g/L-150g/L by the concentration that sodium selenite is configured to sodium selenite, by sub- selenium The amino acid solution that acid sodium solution and mass concentration are 20%-28% is that 1:2-5 is mixed according to volume ratio, is shaken at 35-45 DEG C It swings chelating 48-72h and obtains chelating liquid;
(4) supernatant of step (2) and the chelating liquid of step (3) are mixed according to the ratio of volume 2-5:1, is then added again Enter sodium alkyl benzene sulfonate, be stirred at 25-35 DEG C, secondary chelating 36-48 hours obtains chelating amino acids plant enzyme; The sodium alkyl benzene sulfonate additive amount is 4g/L-8g/L.
Further, the living bacteria count of step (1) probiotics is 2.3 × 109cfu/mL-4.5×109cfu/mL。
During the bionic cultivation of dendrobium candidum, the insect pest rat plague control method of the application are as follows:
Remove that hayashishita weeds, setting mousetrap, binding width is no less than 50 lis on the trunk apart from one meter of ground in time The plastic film of rice, smears a small amount of butter on film.
The invention has the following beneficial effects:
1, the application selects bed cultivation 2 years or more, fresh length is at 20 centimeters or more in the cultivation of dendrobium candidum Fresh item first carries out hardening treatment, can effectively improve the germination rate of base portion, and the growth length of new rudiment is not less than old item, production week Phase is short, and effect is good;The non-woven fabrics of the application is that degradation material does not need to wipe out binding material again in the picking in 1 year of upper tree Material process can save artificial, time cost;In hardening, root is not trimmed;China fir is processed into the batten for retaining bark, is used Non-woven fabrics ties up dendrobium candidum root on China fir item, it is desirable that fir hull is close in root, not only contributes to quick root of hair, is also easy to It sets, saves artificial in binding;Extract on seedbed 2 years raw dendrobium candidums are bundled on China fir item from seedling, it can be effective after hardening The new root of dendrobium candidum is promoted to sprout;When setting in the dendrobium candidum binding after the application hardening, matrix is smeared in wooden face, it is hard It can adhere to dendrobium nobile base portion after change, have that adhesive force is strong, is difficult to be melted by rainwater, be the attachment and dendrobium nobile growth of dendrobium nobile root Provide the effect of nutrition;Meanwhile the dendrobium candidum after the application white silk seedling is easier to be attached to smooth-skined tree than conventional method On wood, such as Chinese anise etc., to improve the growing way and survival rate of dendrobium candidum.
2, the plant enzyme of the application is by dendrobium candidum root, dendrobium candidum leaf, Pachyrhizua angulatus, pineapple, China fir sawdust and probiotics Fermentation is made, in plant enzyme, have after dendrobium candidum root, leaf fermentation the microelements of many suitable dendrobium nobiles growths, amino acid, with And polysaccharide component is precipitated, and dendrobium polysaccharide can be promoted to be enriched in dendrobium candidum body, after Pachyrhizua angulatus fermentation, starch can decompose ammonification A variety of small-molecule substances such as base acid can provide nitrogen source and various trace elements for the dendrobium nobile just transplanted;And after pineapple is fermented, In vitamin be enriched with, can promote dendrobium nobile growth;There is salicylic acid abundant after cedar sawdust fermentation, dendrobium candidum can be inhibited A series of diseases such as stem rot, anthracnose generation, while dendrobium nobile growth can also be effectively facilitated;The plant enzyme of the application at Split-phase is mutually synergistic, to effectively promote Growth of Dendrobium candidum, promotes dendrobium polysaccharide to be enriched in dendrobium candidum body, plays Dendrobium nobile volume increase, the effect for improving dendrobium candidum growing way, promoting dendrobium polysaccharide enrichment.
[Detailed description of the invention]
The schematic diagram that Fig. 1 is bundled when being hardening of the embodiment of the present invention;
Fig. 2 is the schematic diagram of the bionical plantation of dendrobium nobile of the embodiment of the present invention;
Drawing reference numeral explanation: 1, China fir item;2, dendrobium nobile seedling;3, grow nonparasitically upon another plant tree;4, non-woven fabrics;5 matrix.
[specific embodiment]
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, each Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1:
The matrix 5 of the present embodiment comprises the following components in parts by weight: 12 parts of clay, 21 parts of fermentation sheep dung, 7 parts Starch, 5 parts of quick lime, 10 parts of plant enzyme composition;Wherein, starch is glutinous rice starch;Preparation method are as follows: by above-mentioned original After material is sufficiently mixed plus water is kneadinged on one side stir while, and mixture kneading is agglomerating, and hand squeezes the matrix that can be discharged up to the application 5。
Plant enzyme used in the present embodiment is by probiotics, dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple, China fir Sawdust, sodium selenite, amino acid and sodium alkyl benzene sulfonate are made, wherein dendrobium candidum leaf, dendrobium candidum root, pueraria lobata, pineapple are equal For Fresh Plants;Plant enzyme the preparation method comprises the following steps:
(1) strain expands and prepares: brown sugar being mixed with wheat bran according to weight ratio for 1:8, the probiotics of 1mL-3mL is accessed (wherein, the living bacteria count of probiotics is 2.3 × 109Cfu/mL), the warm water with mixture equal volume is added, in temperature It is to ferment 24 hours at 25-29 DEG C, obtains expanding the strain after culture;
(2) dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple and cedar sawdust are mixed according to mass ratio for 3:5:1:2:1 It closes, it is that 1:8 is inoculated with that step (1), which is then expanded the strain after culture and mixture according to mass ratio, it is sealed by fermentation: 90d, It is stand-by to filter to take supernatant;
(3) sodium selenite solution for being 100g/L by the concentration that sodium selenite is configured to sodium selenite, sodium selenite is molten The amino acid solution that liquid and mass concentration are 20%-28% is that 1:2 is mixed according to volume ratio, and concussion chelating 48h is obtained at 35 DEG C To chelating liquid;
(4) supernatant of step (2) and the chelating liquid of step (3) are mixed according to the ratio of volume 2:1, is then added Sodium alkyl benzene sulfonate is stirred at 25 DEG C, and secondary chelating 36 hours obtains chelating amino acids plant enzyme;The alkyl Benzene sulfonic acid sodium salt additive amount is 4g/L.
Foliar fertilizer used in the present embodiment is made by plant enzyme and water according to mass ratio for 1:200.
The dendrobium candidum bionic cultivation method of the present embodiment includes the following steps:
(1) hardening: selection bed plant the time 2 years, fresh length 20cm dendrobium nobile from seedling be dendrobium nobile seedling 2;China fir is processed Leftover pieces afterwards are cut into 5cm wide, the China fir item of 40cm, retain on one side wooden, are on one side bark face;With non-woven fabrics 4 by dendrobium nobile Seedling 2 is fixed on the bark face of China fir item 1, retains 2 root of dendrobium nobile seedling;It is not small that dendrobium candidum quantity is bundled on the China fir item 1 In 3 clumps;It waters to the China fir item 1 after dendrobium nobile seedling 2 has been bundled, the 1st day, watering 1 time daily, the 3rd day, watering was primary daily, and the 7th It is primary every watering in 2 days after it;It is grown to new root, terminates hardening;
(2) attached base: retain wooden face to the China fir item 1 after step (1) hardening and smear the base that a layer thickness is 0.5cm Matter 5 obtains Growth of Dendrobium candidum matrix after the hardening of matrix 5;
(3) growing substrate of step (2) bionical plantation: is bundled into the corresponding portion of pine tree (grow nonparasitically upon another plant tree 3) with non-woven fabrics 4 Position;Pine tree (grow nonparasitically upon another plant tree 3) is trimmed, guarantees that the closing of its leaf are 40%-70%;During plantation, every month is to iron sheet Dendrobium nobile foliage spray foliar fertilizer.
During the bionic cultivation of dendrobium candidum, the insect pest rat plague control method of the application are as follows:
Remove that hayashishita weeds, setting mousetrap, binding width is no less than 50 lis on the trunk apart from one meter of ground in time The plastic film of rice, smears a small amount of butter on film.
Embodiment 2:
The matrix 5 of the present embodiment comprises the following components in parts by weight: 18 parts of clay, 27 parts of fermentation sheep dung, 17 parts Starch, 15 parts of quick lime, 17 parts plant enzyme composition;Wherein, starch is that flour and tapioca are according to mass ratio 1:1 is obtained by mixing;Preparation method are as follows: after being sufficiently mixed above-mentioned raw materials plus water is kneadinged on one side stir while, and mixture is rubbed Pinch agglomerating, the crowded matrix 5 that can be discharged up to the application of hand.
Plant enzyme used in the present embodiment is by probiotics, dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple, China fir Sawdust, sodium selenite, amino acid and sodium alkyl benzene sulfonate are made, wherein dendrobium candidum leaf, dendrobium candidum root, pueraria lobata, pineapple are equal For Fresh Plants;Plant enzyme the preparation method comprises the following steps:
(1) strain expands and prepares: brown sugar being mixed with wheat bran according to weight ratio for 1:12, probiotics (its of 3mL is accessed In, the living bacteria count of probiotics is 4.5 × 109Cfu/mL), the warm water with mixture equal volume is added, is in temperature It ferments 24 hours at 29 DEG C, obtains expanding the strain after culture;
(2) dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple and cedar sawdust are mixed according to mass ratio for 4:6:3:5:3 It closes, it is that 1:14 is inoculated with that step (1), which is then expanded the strain after culture and mixture according to mass ratio, it is sealed by fermentation 95d, It is stand-by to filter to take supernatant;
(3) sodium selenite solution for being 150g/L by the concentration that sodium selenite is configured to sodium selenite, sodium selenite is molten The amino acid solution that liquid and mass concentration are 20%-28% is that 1:5 is mixed according to volume ratio, and concussion chelating 72h is obtained at 45 DEG C To chelating liquid;
(4) supernatant of step (2) and the chelating liquid of step (3) are mixed according to the ratio of volume 5:1, is then added Sodium alkyl benzene sulfonate is stirred at 35 DEG C, and secondary chelating 48 hours obtains chelating amino acids plant enzyme;The alkyl Benzene sulfonic acid sodium salt additive amount is 8g/L.
Foliar fertilizer used in the present embodiment is made by plant enzyme and water according to mass ratio for 1:300.
The dendrobium candidum bionic cultivation method of the present embodiment includes the following steps:
(1) hardening: selection bed plant the time 3 years, fresh length 22cm dendrobium nobile from seedling be dendrobium nobile seedling 2;China fir is processed Leftover pieces afterwards are cut into 5cm wide, the China fir item 1 of 50cm, retain on one side wooden, are on one side bark face;With non-woven fabrics 4 by dendrobium nobile Seedling 2 is fixed on the bark face of China fir item 1, retains 2 root of dendrobium nobile seedling;It is not small that dendrobium candidum quantity is bundled on the China fir item 1 In 3 clumps;It waters to the China fir item 1 after dendrobium nobile seedling 2 has been bundled, the 2nd day, watering 2 times daily, the 5th day, watering was primary daily, and the 7th It is primary every watering in 3 days after it;It is grown to new root, terminates hardening;
(2) attached base: retain wooden face to the China fir item 1 after step (1) hardening and smear the matrix that a layer thickness is 1cm 5, Growth of Dendrobium candidum matrix is obtained after the hardening of matrix 5;
(3) growing substrate of step (2) bionical plantation: is bundled into the corresponding portion of Chinese chestnut tree (grow nonparasitically upon another plant tree 3) with non-woven fabrics 4 Position;Chinese chestnut tree (grow nonparasitically upon another plant tree 3) is trimmed, guarantees that the closing of its leaf are 40%-70%;During plantation, every month is to iron Skin dendrobium nobile foliage spray foliar fertilizer.
During the bionic cultivation of dendrobium candidum, the insect pest rat plague control method of the application are as follows:
Remove that hayashishita weeds, setting mousetrap, binding width is no less than 50 lis on the trunk apart from one meter of ground in time The plastic film of rice, smears a small amount of butter on film.
Embodiment 3:
The matrix 5 of the present embodiment comprises the following components in parts by weight: 16 parts of clay, 23 parts of fermentation sheep dung, 12 parts Starch, 8 parts of quick lime, 14 parts plant enzyme composition;Wherein, starch is that glutinous rice starch, flour and potato starch are pressed It is obtained by mixing according to 1:1:1;Preparation method are as follows: after being sufficiently mixed above-mentioned raw materials plus water is kneadinged on one side stir while, will be mixed Object kneading is agglomerating, and hand squeezes the matrix 5 that can be discharged up to the application.
Plant enzyme used in the present embodiment is by probiotics, dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple, China fir Sawdust, sodium selenite, amino acid and sodium alkyl benzene sulfonate are made, wherein dendrobium candidum leaf, dendrobium candidum root, pueraria lobata, pineapple are equal For Fresh Plants;Plant enzyme the preparation method comprises the following steps:
(1) strain expands and prepares: brown sugar being mixed with wheat bran according to weight ratio for 1:10, probiotics (its of 2mL is accessed In, the living bacteria count of probiotics is 3.5 × 109Cfu/mL), the warm water with mixture equal volume is added, is in temperature It ferments 24 hours at 27 DEG C, obtains expanding the strain after culture;
(2) dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple and cedar sawdust are mixed according to mass ratio for 4:6:2:3:2 It closes, it is that 1:8-14 is inoculated with that step (1), which is then expanded the strain after culture and mixture according to mass ratio, is sealed by fermentation It is stand-by to filter to take supernatant by 92d;
(3) sodium selenite solution for being 120g/L by the concentration that sodium selenite is configured to sodium selenite, sodium selenite is molten The amino acid solution that liquid and mass concentration are 20%-28% is that 1:3 is mixed according to volume ratio, and concussion chelating 52h is obtained at 38 DEG C To chelating liquid;
(4) supernatant of step (2) and the chelating liquid of step (3) are mixed according to the ratio of volume 3:1, is then added Sodium alkyl benzene sulfonate is stirred at 30 DEG C, and secondary chelating 41 hours obtains chelating amino acids plant enzyme;The alkyl Benzene sulfonic acid sodium salt additive amount is 5g/L.
Foliar fertilizer used in the present embodiment is made by plant enzyme and water according to mass ratio for 1:250.
The dendrobium candidum bionic cultivation method of the present embodiment includes the following steps:
(1) hardening: selection bed plant the time 3 years, fresh length 24cm dendrobium nobile from seedling be dendrobium nobile seedling 2;China fir is processed Leftover pieces afterwards are cut into 5cm wide, the China fir item 1 of 45cm, retain on one side wooden, are on one side bark face;With non-woven fabrics 4 by dendrobium nobile Seedling 2 is fixed on the bark face of China fir item 1, retains 2 root of dendrobium nobile seedling;It is not small that dendrobium candidum quantity is bundled on the China fir item 1 In 3 clumps;It waters to the China fir item 1 after dendrobium nobile seedling 2 has been bundled, the 1st day, watering 2 times daily, the 3rd day, watering was primary daily, and the 7th It is primary every watering in 3 days after it;It is grown to new root, terminates hardening;
(2) attached base: retain wooden face to the China fir item 1 after step (1) hardening and smear the base that a layer thickness is 0.8cm Matter 5 obtains Growth of Dendrobium candidum matrix after the hardening of matrix 5;
(3) growing substrate of step (2) bionical plantation: is bundled into the corresponding portion of Chinese anise (grow nonparasitically upon another plant tree 3) with non-woven fabrics 4 Position;Qinggang wooden (grow nonparasitically upon another plant tree 3) is trimmed, guarantees that the closing of its leaf are 50%;During plantation, every month is to dendrobium candidum Foliage spray foliar fertilizer.
During the bionic cultivation of dendrobium candidum, the insect pest rat plague control method of the application are as follows:
Remove that hayashishita weeds, setting mousetrap, binding width is no less than 50 lis on the trunk apart from one meter of ground in time The plastic film of rice, smears a small amount of butter on film.
Control group 1:
Plant enzyme is not contained in the matrix 5 of this control group, i.e., the matrix 5 of this control group is by following parts by weight at grouping At: 12 parts of clay, 21 parts of fermentation sheep dung, 7 parts of starch, 5 parts of quick lime, 10 parts of plant enzyme composition;Wherein, it forms sediment Powder is glutinous rice starch;Other embodiment and embodiment 1 are completely the same.
Control group 2:
Starch is not contained in the matrix 5 of this control group, i.e. the matrix 5 of this control group comprises the following components in parts by weight: 12 parts of clay, 21 parts of fermentation sheep dung, 7 parts of starch, 5 parts of quick lime, 10 parts of plant enzyme composition;Wherein, starch For glutinous rice starch;Other embodiment and embodiment 1 are completely the same.
Control group 3:
This control group does not spray foliar fertilizer to dendrobium candidum, but sprays distilled water, and other embodiment and embodiment 1 are complete It is complete consistent.
Control group 4:
Dendrobium candidum seedling 2 is directly tied up and is being grown nonparasitically upon another plant on tree living without China fir hardening treatment by this control group, other Embodiment and embodiment 1 are completely the same.
Control group 5:
This control group does not use matrix 5, but will directly tie up and grown nonparasitically upon another plant on tree living after 2 hardening of dendrobium candidum seedling, Qi Tashi It applies mode and embodiment 1 is completely the same.
Control group 6:
This control group without using matrix 5, without using the foliar fertilizer of the application, without China fir hardening, directly by iron sheet Dendrobium nobile seedling 2, which is tied up, is growing nonparasitically upon another plant on tree living, and other embodiment and embodiment 1 are completely the same.
Test experiments:
It tests place: being carried out in the Experimental Base of our unit;
Experimental period: in July, -2018 in 2017
Experimental method: the Experimental Base of our unit 9 pieces of samples are set, each sample area is 500m2, according to embodiment The method of 1-3 and control group 1-6 are planted, and in August, 2017 plants iron sheet in the way of embodiment 1-3 and control group 1-6 Dendrobium nobile, in May, 2018, June carry out stem length, stem thickness, the measurement of joint number, investigation to the dendrobium candidum plant in growth period respectively, The content for taking the fresh strain measurement Dendrobium officinale polysaccharide of dendrobium candidum of each processing at random in July, 2018, the results are shown in Table 1:
Table 1
As seen from the above table, the stem length, stem thickness of embodiment 1-3 is above control group 1-6, illustrates that the cultural method of the application can Effectively improve stem length, the stem thickness of dendrobium nobile;The dendrobium polysaccharide content of embodiment 1-3 is apparently higher than control group 1, control group 3, control group 6, illustrate that the plant enzyme of the application can effectively improve dendrobium polysaccharide content, wherein and the bark of growing nonparasitically upon another plant of embodiment 1-2 is coarse, And the bark of growing nonparasitically upon another plant of embodiment 3 is smooth, but its stem length, stem thickness, joint number and dendrobium polysaccharide influence less, to illustrate to use this Shen Method please can be improved dendrobium candidum and imitate the range of choice that man of wild origin plants.
In conclusion the implantation methods using the application can effectively overcome in the bionical planting process of dendrobium candidum, because For open environment, serious, the uncontrollable technological deficiency of environment occurs for various pest and disease damages, is a kind of high and low cost of adaptability, yield Height, high survival rate, dendrobium polysaccharide content height, the good officinal dendrobium stem plantation method easy to spread of quality.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims (9)

1. a kind of dendrobium candidum bionic cultivation method, which is characterized in that described method includes following steps:
(1) hardening: selection bed plant the time be greater than 2 years, fresh length greater than 20cm dendrobium nobile from seedling be dendrobium nobile seedling;By China fir plus Leftover pieces after work are cut into 5cm wide, the China fir item of 40-50cm, retain on one side wooden, are on one side bark face;It will with non-woven fabrics Dendrobium nobile seedling is fixed on the bark face of China fir item, retains dendrobium nobile seedling root;It is not small that dendrobium candidum quantity is bundled on the China fir item In 3 clumps;It to the watering of the China fir item after dendrobium nobile seedling has been bundled, the 1-2 days, waters 1-2 times daily, the 3-5 days, waters one daily It is secondary, it is primary every watering in 2-3 days after the 7th day;It is grown to new root, terminates hardening;
(2) attached base: retaining wooden face to the China fir item after step (1) hardening and smear the matrix that a layer thickness is 0.5-1cm, Growth of Dendrobium candidum matrix is obtained after matrix hardening;
(3) growing substrate of step (2) bionical plantation: is bundled into the corresponding site for the tree living that grows nonparasitically upon another plant with non-woven fabrics;To the tree that grows nonparasitically upon another plant It is trimmed, guarantees that the closing of its leaf are 40%-70%;During plantation, every month is to dendrobium candidum foliage spray foliar fertilizer.
2. a kind of dendrobium candidum bionic cultivation method according to claim 1, which is characterized in that the step (3) it is attached Raw tree is preferably Qinggang wood, pine tree or Chinese chestnut tree.
3. a kind of dendrobium candidum bionic cultivation method according to claim 1, which is characterized in that the base of the step (2) Matter comprises the following components in parts by weight: 12 parts -18 parts of clay, 21 parts -27 parts of fermentation sheep dung, 7 parts -17 parts of starch, 5 The quick lime of -15 parts of part, 10 parts -17 parts of plant enzyme composition.
4. a kind of dendrobium candidum bionic cultivation method according to claim 1, which is characterized in that the leaf of the step (3) Leaven is made by plant enzyme and water according to mass ratio for 1:200-300.
5. a kind of dendrobium candidum bionic cultivation method according to claim 3, which is characterized in that the starch is glutinous rice shallow lake The combination of one or more of powder, flour, tapioca and/or potato starch.
6. a kind of dendrobium candidum bionic cultivation method according to claim 3 or 4 any one, which is characterized in that described Plant enzyme is by probiotics, dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple, cedar sawdust, sodium selenite, amino acid and alkyl Benzene sulfonic acid sodium salt is made.
7. a kind of dendrobium candidum bionic cultivation method according to claim 6, which is characterized in that the dendrobium candidum leaf, Dendrobium candidum root, pueraria lobata, pineapple are Fresh Plants.
8. a kind of dendrobium candidum bionic cultivation method according to claim 6, which is characterized in that the system of the plant enzyme Preparation Method are as follows:
(1) strain expands and prepares: brown sugar is mixed with wheat bran according to weight ratio for 1:8-12, the probiotics of 1mL-3mL is accessed, The warm water with mixture equal volume is added, ferments 24 hours at being 25-29 DEG C in temperature, obtains expanding the bacterium after culture Kind;
(2) it is 3-4:5-6:1-3:2-5 according to mass ratio by dendrobium candidum root, dendrobium candidum leaf, pueraria lobata, pineapple and cedar sawdust: 1-3 mixing, it is that 1:8-14 is inoculated with that step (1), which is then expanded the strain after culture and mixture according to mass ratio, is sealed Fermentation: it is stand-by to filter to take supernatant by 90-95d;
(3) sodium selenite solution for being 100g/L-150g/L by the concentration that sodium selenite is configured to sodium selenite, by sodium selenite The amino acid solution that solution and mass concentration are 20%-28% is that 1:2-5 is mixed according to volume ratio, shakes chela at 35-45 DEG C It closes 48-72h and obtains chelating liquid;
(4) supernatant of step (2) and the chelating liquid of step (3) are mixed according to the ratio of volume 2-5:1, then adds alkane Base benzene sulfonic acid sodium salt is stirred at 25-35 DEG C, and secondary chelating 36-48 hours obtains chelating amino acids plant enzyme;It is described Sodium alkyl benzene sulfonate additive amount is 4g/L-8g/L.
9. a kind of dendrobium candidum bionic cultivation method according to claim 8, which is characterized in that the step (1) is prebiotic The living bacteria count of bacterium is 2.3 × 109cfu/mL-4.5×109cfu/mL。
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113207566A (en) * 2021-05-19 2021-08-06 广东兴源农林有限公司 Method for binding and cultivating dendrobium officinale broadleaf living tree to prevent damage of rats
CN114303916A (en) * 2021-12-31 2022-04-12 西南林业大学 Organic planting method for dendrobium
CN114342790A (en) * 2021-12-16 2022-04-15 浙江高鼻子生物科技有限公司 Cultivation method for increasing anthocyanin content of dendrobium officinale and special device
CN115005075A (en) * 2022-07-15 2022-09-06 贵州省亚热带作物研究所 Planting method for dendrobium nobile by nailing on stone surface in karst region

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102499038A (en) * 2011-10-24 2012-06-20 浙江雁荡山生态铁皮石斛开发有限公司 Tree tissue culture method of dendrobium candidum
CN103304315A (en) * 2013-06-17 2013-09-18 厦门鹰君药业有限公司 Fertilizer for planting dendrobium officinale in epiphytic manner, preparation method thereof and method for planting dendrobium officinale in epiphytic manner
CN103858738A (en) * 2014-03-25 2014-06-18 福建九益堂药业有限公司 Planting method for pasting dendrobium officinale hood pocket to tree
CN103918551A (en) * 2014-01-16 2014-07-16 贵州清控置业有限公司 Imitation wild cultivation method of mycorrhizated dendrobium plant tea tree
CN105875167A (en) * 2016-05-19 2016-08-24 云南斛健庄园生物科技有限公司 Symbiotic planting method for ancient tea trees and dendrobium
CN106045667A (en) * 2016-05-27 2016-10-26 广西壮族自治区农业科学院农业资源与环境研究所 Preparation method and application of liquid selenium-rich fertilizer
CN107509615A (en) * 2017-07-26 2017-12-26 河南联源生物科技有限公司 Wild-imitating cultivation method for dendrobium officinale tissue culture seedlings

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102499038A (en) * 2011-10-24 2012-06-20 浙江雁荡山生态铁皮石斛开发有限公司 Tree tissue culture method of dendrobium candidum
CN103304315A (en) * 2013-06-17 2013-09-18 厦门鹰君药业有限公司 Fertilizer for planting dendrobium officinale in epiphytic manner, preparation method thereof and method for planting dendrobium officinale in epiphytic manner
CN103918551A (en) * 2014-01-16 2014-07-16 贵州清控置业有限公司 Imitation wild cultivation method of mycorrhizated dendrobium plant tea tree
CN103858738A (en) * 2014-03-25 2014-06-18 福建九益堂药业有限公司 Planting method for pasting dendrobium officinale hood pocket to tree
CN105875167A (en) * 2016-05-19 2016-08-24 云南斛健庄园生物科技有限公司 Symbiotic planting method for ancient tea trees and dendrobium
CN106045667A (en) * 2016-05-27 2016-10-26 广西壮族自治区农业科学院农业资源与环境研究所 Preparation method and application of liquid selenium-rich fertilizer
CN107509615A (en) * 2017-07-26 2017-12-26 河南联源生物科技有限公司 Wild-imitating cultivation method for dendrobium officinale tissue culture seedlings

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李禾: "《科普经典——身边的环保》", 31 July 2013, 中国华侨出版社 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113207566A (en) * 2021-05-19 2021-08-06 广东兴源农林有限公司 Method for binding and cultivating dendrobium officinale broadleaf living tree to prevent damage of rats
CN114342790A (en) * 2021-12-16 2022-04-15 浙江高鼻子生物科技有限公司 Cultivation method for increasing anthocyanin content of dendrobium officinale and special device
CN114303916A (en) * 2021-12-31 2022-04-12 西南林业大学 Organic planting method for dendrobium
CN115005075A (en) * 2022-07-15 2022-09-06 贵州省亚热带作物研究所 Planting method for dendrobium nobile by nailing on stone surface in karst region
CN115005075B (en) * 2022-07-15 2023-03-10 贵州省亚热带作物研究所 Planting method for dendrobium nobile by nailing on stone surface in karst region

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