CN107509615A - Wild-imitating cultivation method for dendrobium officinale tissue culture seedlings - Google Patents
Wild-imitating cultivation method for dendrobium officinale tissue culture seedlings Download PDFInfo
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- CN107509615A CN107509615A CN201710619725.2A CN201710619725A CN107509615A CN 107509615 A CN107509615 A CN 107509615A CN 201710619725 A CN201710619725 A CN 201710619725A CN 107509615 A CN107509615 A CN 107509615A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a wild-imitating cultivation method for dendrobium officinale tissue culture seedlings, which comprises the following steps: selecting seedlings, airing the seedlings, cutting plates, planting the seedlings, fixing roots and transplanting. Selecting, cleaning and airing seedlings, directly planting and binding the seedlings on a cut plate with bark, then intensively hanging the seedlings in a domestication greenhouse for domestication, and transplanting the domesticated dendrobium officinale tissue culture seedlings and the plate with the bark to a proper field environment for wild-like growth after the dendrobium officinale tissue culture seedlings are rooted. The process saves the link that tissue culture seedlings must be domesticated by a greenhouse seedbed in the traditional method, reduces production procedures, saves a large amount of investment of manpower, material resources and financial resources, greatly reduces production cost and shortens production time; the wild-like cultivation has high survival rate and wide transplantable range, and the method is simple, convenient, highly operable, safe, reliable, high in survival rate and greatly reduced in late-stage seedling supplementing phenomenon.
Description
Technical field
The present invention relates to the imitating wildness cultural technique field of dendrobium candidum, and in particular to a kind of candidum tissue culturing seedling is imitated
Wild cultivating method.
Background technology
Dendrobium candidum is rare rare traditional Chinese medicine, is known as " a thousand pieces of gold grass ", " plant gold ", the title of " healthy soft gold ", because
Its is divinely efficacious, extremely rare, living environment is special and distinguished nourishing effects and rank first of " Chinese nine big celestial grass ", by state
Border medicinal plant circle is referred to as " medicine circle giant panda ".Particularly nourishing Yin and moistening lung, nourishing the stomach to improve the production of body fluid, brain nourishing and eyesight improving, the consumptive disease of tonifying five zang organs；It is right
Excessive drinking and smoking, overwork, night life excessively, with eye is overstrain one's nerves, vocal cords are hoarse etc. has special efficacy；It is thin to the lung cancer of people
Born of the same parents, atrophic gastritis, diabetes have great inhibitory action, and inhibiting rate is up to 74.7%~97.2%, and can be effectively reduced
Blood glucose, a new therapy approach is opened for numerous diabetics, is paid attention to by medical field！
However, dendrobium candidum because it is magical with unique the effect of, at home and abroad supply falls short of demand in market, for a long time, Ren Menyong
This medicine is all to collect wild resource by excavation, and the temptation of juice makes dendrobium officinale be planted as world's Precious, Rare, Endangered
Thing, thus by the attention and protection of country.In order to realize the sustainable use of dendrobium candidum resource, scientific research personnel is from 20th century
Begin to artificial cultivation technique research work the seventies, but until this century, seed production, tissue cultures and facility cultivation etc.
Artificial breeding key technology is just broken through, and rapid popularization and application.At present, on candidum tissue culturing industrial seedling rearing ring
Section, technology is gradually mature and stable, because dendrobium candidum is carried out in artificial environment in tissue culture propagation, its medicinal valency
Value is far away from wild dendrobium candidum, therefore necessary use imitates wild method plantation dendrobium candidum, but for iron sheet stone
Dry measure used in former times pseudo-wild cultivating method, various technological deficiencies and drawback also be present mostly at present.
Publication No. 105960973A patent of invention discloses a kind of Dendrobidium huoshanness natural as disclosed in September in 2016 28 days
The cultural method of sylvan life, it is disadvantageous in that：Need just carry out grafting in annual May, fair weather, by branch from
Cut with trunk junction, and the otch progress grafting of a triangle is cut at trunk；It is public disclosed in 4 days January in 2017
The number of opening is that 106258885A patent of invention discloses a kind of dendrobium candidum pseudo-wild cultivating method, is that the stem of noble dendrobium is fixed on 1 year
More than mao bamboon grown nonparasitically upon another plant as dendrobium candidum the carrier of cultivation, the lunar calendar October, dendrobium candidum seedling is fixed on to the bamboo of mao bamboon
Section top；Publication No. 106376443A patent of invention disclosed in 8 days discloses a kind of imitative open country of dendrobium candidum within 2 months 2017
Agricultural climate condition for corn method, it selects to erect wood to be withered, stem of noble dendrobium directly transplanting is fixed on setting wood, and every layer plants 10 young plants, along standing
Wooden ring-type is averagely arranged, and is carried out artificially applying fertilizer management, is simply copied wild Dendrodium growth part feature, be actually field planting,
And it is directly to be planted seedlings in the wild with above-mentioned two situations, by natural environment influence, time restriction of planting seedlings is larger, and
And survival rate is low.
Above-mentioned various methods have disadvantages in that jointly：1）Program is numerous and diverse, and operation inconvenience, efficiency is low, special
Carry out bundling tree under conditions of unfavorable in the wild（Such as height of tree, slope are steep, cliff）, operate and implement difficult,
It is time consuming；2）Survival rate is low, and cost is high, directly carries out setting in grafting or binding in the wild, the death rate is high, slow-growing, stone
Dry measure used in former times seedling wastes seriously, and follow-up workload of filling the gaps with seedlings is big, serious to increase human and material resources and financial resources cost；3）Pseudo-wild cultivating field
Institute's limitation is big, and not every trees or cliff are suitable for, such as the trees with grease or gel, smooth-skined tree
Wood or cliff etc., conventional method transplanting is unsuitable for, is not easy to wide popularization and application.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of easy, quick, workable, cost is low, survival rate is high,
Securely and reliably, the pseudo-wild cultivating method of the candidum tissue culturing seedling of wide adaptation range, requires no candidum tissue culturing seedling
This link is tamed with regard to that can rapidly adapt to natural environment in greenhouse seedbed, and when being transferred in wild environment, without moving root, root
Fasten admittedly, survival rate is greatly improved, it is ensured that the security and operability of dendrobium candidum pseudo-wild cultivating.
In order to solve the above technical problems, the present invention is adopted the following technical scheme that to realize：
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps：
A. seedling is selected：Select and cultivated at least 60 days in culturing room, and the astigmatism hardening candidum tissue culturing of at least 30 days in greenhouse
Seedling, preferably more than plant height 5cm, there is 4-6 strains section, the root of more than 3, stalk is sturdy, leaf color is dark green seedling more preferably；
B. dry in the air seedling：Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby；
C. sheet material is cut：Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu,
The belt leather timber such as pine tree, willow, wide 10-30cm, long 20-50cm, center thickness 3-10cm belt leather sheet material are cut into, it is standby；
D. plant seedlings：Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 2-6 strain, step C is laid in from for any per 2-4
The belt leather side of gained sheet material, and in covering moss on every bit, bundled using the rope made of hemp by above-mentioned moss and candidum tissue culturing seedling
It is fixed on the sheet material, it is desirable to elastic moderate；
E. root is determined：Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, keeps bark moist, in
Tame domestication more than 10 days in grid；
F. transplant：The sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, and set spray or
Watering system, often watering, keeps humidity.
In the step D, spacing of the candidum tissue culturing seedling on panel length direction is 8-12cm.
In the step D, the rope made of hemp is on each point in its middle and lower part Chan Ya 2-4 roads.
In the step E, the root water sprayed once every 7-12 days, continuously sprayed 2-5, tamed humid control in grid
In 70-85%.
In the step F, determining root growth has the sheet material of candidum tissue culturing seedling solid by hanging or bundling or following closely nail etc.
Determine mode, be colonized in tree above or on cliff.
The root water is the mixed aqueous solution of thiamine hydrochloride, puridoxine hydrochloride, 3-indolyl acetic acid and water.
The mass ratio of the thiamine hydrochloride, puridoxine hydrochloride and 3-indolyl acetic acid is（80-100）﹕（4-6）﹕（1-3）.
The canopy density in the forest land are 55-65%.
The present invention is relative to the positive beneficial effect acquired by prior art：
The candidum tissue culturing seedling pseudo-wild cultivating method of the application, using selecting seedling, cleaning after seedling of drying in the air, directly field planting, which is bundled in, cuts
On the sheet material with bark cut, then concentrate to be suspended in domestication greenhouse and tame, will after candidum tissue culturing seedling determines root
The candidum tissue culturing seedling and belt leather sheet material tamed are transplanted under suitable wild environment together to carry out imitating wild growth.It is first
First, eliminate tissue-cultured seedling in conventional method and have to pass through greenhouse seedbed and tame this link, reduce production process, save big
Human and material resources and the financial resources input of amount, greatly reduce production cost, shorten the production time；Second, pass through the China fir in belt leather
The direct domestication of candidum tissue culturing seedling and transplanting, one side production efficiency are significantly improved on tree plate, on the other hand, avoided more
The fracture of candidum tissue culturing seedling root system and damage caused by secondary transplanting, stable beneficial to candidum tissue culturing seedling grow, greatly
Improve the survival rate of pseudo-wild cultivating；3rd, the present invention, to the direct domestication of candidum tissue culturing seedling, tames and dociles on the sheet material of belt leather
After the completion of change, the transplantable more other conventional methods of scope are broad, under conditions of environment is suitable, will carry dendrobium candidum seedling
Sheet material, by hanging, bundling or following closely the fixed forms such as nail, be colonized in that tree is upper or cliff on, which greatly enhances trees and
The utilization rate in space, increase the production yields of unit area, at the same time it can also prevent ground pest and disease damage.
The root water of the application uses the mixed aqueous solution of thiamine hydrochloride, puridoxine hydrochloride and 3-indolyl acetic acid, and it is rich
Containing B family vitamin, stem, leaf development quality and the growth coefficient of candidum tissue culturing seedling are remarkably improved, there is effective powerful
It cell-activating function, can penetrate into plant rapidly, promote the plasm of cell to flow, improve cell viability, promote life
Long development, the survival rate and growth quality of tissue-cultured seedling are significantly improved, wherein, heteroauxin can also effectively facilitate dendrobium candidum fibrous root
Growth.
The inventive method is simple, convenient, workable, and security reliability is good, and survival rate is high, both improves production efficiency
Reduce production cost again, later stage phenomenon of filling the gaps with seedlings greatly is reduced, and has preferable economic benefit, social benefit and Ecological Effect
Benefit, should be widely promoted application.
Brief description of the drawings
The present invention is described further below in conjunction with the accompanying drawings：
Fig. 1 is the sheet material schematic diagram that the present invention is bundled with candidum tissue culturing seedling；
Fig. 2 is the integrated operation schematic flow sheet of the present invention；
1. ropes made of hemp, 2. sheet material xylems, 3. sheet material barks, 4. mosses, 5. candidum tissue culturing seedlings, 6. fixed mechanisms in figure.
Embodiment
Referring to Fig. 1 and 2, technical solution of the present invention is further elaborated on below in conjunction with accompanying drawing.
Embodiment one
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps：
A. seedling is selected：Select and cultivated 60 days in culturing room, and astigmatism hardening 30 days in greenhouse, more than bottle outlet tissue-cultured seedling plant height 5cm,
With 4-6 strain sections, the root of more than 3, stalk is sturdy, the dark green candidum tissue culturing seedling of leaf color；
B. dry in the air seedling：Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby；By the seedling that dries in the air,
The hydrophily of candidum tissue culturing seedling root system can be effectively improved, but is attached in its root system on the timber to plant seedlings, root effect is determined in raising
Fruit and survival rate；
C. sheet material is cut：Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu,
The belt leather timber such as pine tree, willow, wide 10cm, long 20cm, center thickness 3cm belt leather sheet material are cut into, as shown in figure 1, sheet material has
There are sheet material xylem 2 and sheet material bark 3, the side of upper board material xylem 2 is provided with fixed mechanism 6, and fixed mechanism 6 is hook or hangs
Ring or iron nail, it is standby to prepare sheet material；
D. plant seedlings：Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 2-3 strain, as shown in figure 1, per 3-4 from for some cloth
Located at the belt leather side of sheet material obtained by step C, every 3 points are one layer, and the candidum tissue culturing seedling of adjacent two layers is in panel length side
Upward spacing is 8-10cm, by control room away from, both be its production retain sufficient space, again be beneficial to effectively improve yield；In
Moss 4 is covered on every bit, moss 4 is covered in the root of candidum tissue culturing seedling 5, is bundled using the rope made of hemp 1 by the above-mentioned He of moss 4
Candidum tissue culturing seedling 5 is fixed on the sheet material, and the rope made of hemp 4 twines pressure 2 on each point in its middle and lower part, on the one hand can carry
The fixed reliability of height, is on the other hand easy to the later stage to store moisture, and beneficial to the growth of tissue-cultured seedling, it is elastic moderate to twine pressure request, with
Moss 4 and candidum tissue culturing seedling 5 are compressed, it is dropped, meanwhile, not breaking or damage tissue-cultured seedling and its root system is advisable；
E. root is determined：Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, and root water is hydrochloric acid sulphur
Amine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water, the thiamine hydrochloride, puridoxine hydrochloride and 3- indoles
The mass ratio of acetic acid is the ﹕ 1 of 80 ﹕ 4, and root water was sprayed once every 7-8 days, continuously sprayed 3 times, tames humid control in grid and exists
70-85%.Keep bark moist, tamed 15 days in domestication grid；
F. the sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, selected close to water source, it is frostless
Foggy, at the larger forest land of air humidity or cliff, the canopy density in forest land are 55-65%, by hanging or bundling or following closely nail
Etc. fixed form, tree is colonized in above or on cliff, and spray or watering system are set, often spray or watering, holding humidity.
Final-period management points for attention：When in the morning or afternoon, illumination was weaker, often watering, humidity is kept.Meanwhile
Pay attention to repairing and subtract branch, adjust the canopy density of the woods.Pay attention to the prevention and control of plant diseases, pest control work, adhere to " can not medication, just not medication, prevention
Based on, integrated control " principle, it is necessary to during medication, strict quarantine is taken, typically with Bravo, thiram, methyl Tobe
The preventing and treating stem of noble dendrobium such as Tianjin, zineb, carbendazim black rot, anthracnose, leaf blight, gray mold, droop etc., with close up to preventing and treating snail
Ox, slug.
The present embodiment pseudo-wild cultivating survival rate is 96.6%.
Embodiment two
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps：
A. seedling is selected：Select and cultivated 65 days in culturing room, and astigmatism hardening 40 days in greenhouse, more than plant height 5cm, there is 4-6 strains
Section, the root of more than 3, the candidum tissue culturing seedling that stalk is sturdy, leaf color is dark green
B. dry in the air seedling：Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby；
C. sheet material is cut：Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu,
The belt leather timber such as pine tree, willow, wide 20cm, long 30cm, center thickness 6cm belt leather sheet material are cut into, as shown in figure 1, sheet material has
There are sheet material xylem 2 and sheet material bark 3, the side of upper board material xylem 2 is provided with fixed mechanism 6, and fixed mechanism 6 is hook or hangs
Ring or iron nail, it is standby to prepare sheet material；
D. plant seedlings：Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 3-4 strain, as shown in figure 1, per 2-3 from for some cloth
Located at the belt leather side of sheet material obtained by step C, every 4 points are one layer, and the candidum tissue culturing seedling of adjacent two layers is in panel length side
Upward spacing is 8-10cm, and in covering moss 4 on every bit, moss 4 covers in the root of candidum tissue culturing seedling 5, used
The rope made of hemp 1, which bundlees, is fixed on above-mentioned moss 4 and candidum tissue culturing seedling 5 on the sheet material, and the rope made of hemp 4 is on each point under wherein
Portion twines pressure 3；
E. root is determined：Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, and root water is hydrochloric acid sulphur
Amine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water, the thiamine hydrochloride, puridoxine hydrochloride and 3- indoles
The mass ratio of acetic acid is the ﹕ 2 of 90 ﹕ 5, and root water sprayed once every 10 days, continuously sprays 2, and humid control is in 70- in domestication grid
85%.Keep bark moist, tamed 30 days in domestication grid；
F. the sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, selected close to water source, it is frostless
Foggy, at the larger forest land of air humidity or cliff, the canopy density in forest land are 55-65%, by hanging or bundling or following closely nail
Etc. fixed form, tree is colonized in above or on cliff, and spray or watering system are set, often spray or watering, holding humidity.
With embodiment one, pseudo-wild cultivating survival rate is 98.7% for the present embodiment final-period management.
Embodiment three
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps：
A. seedling is selected：Select and cultivated 70 days in culturing room, and the astigmatism hardening candidum tissue culturing seedling of 50 days in greenhouse；
B. dry in the air seedling：Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby；
C. sheet material is cut：Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu,
The belt leather timber such as pine tree, willow, wide 30cm, long 50cm, center thickness 10cm belt leather sheet material are cut into, as shown in figure 1, sheet material has
There are sheet material xylem 2 and sheet material bark 3, the side of upper board material xylem 2 is provided with fixed mechanism 6, and fixed mechanism 6 is hook or hangs
Ring or iron nail, it is standby to prepare sheet material；
D. plant seedlings：Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 5-6 strain, as shown in figure 1, per 2-3 from for some cloth
It it is one layer per 4-5 point, the candidum tissue culturing seedling of adjacent two layers is in panel length located at the belt leather side of sheet material obtained by step C
Spacing on direction is 10-12cm, and in covering moss 4 on every bit, moss 4 covers in the root of candidum tissue culturing seedling 5, adopted
Bundled with the rope made of hemp 1 and above-mentioned moss 4 and candidum tissue culturing seedling 5 are fixed on the sheet material, the rope made of hemp 4 is on each point in wherein
Bottom twines pressure 4；
E. root is determined：Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, and root water is hydrochloric acid sulphur
Amine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water, the thiamine hydrochloride, puridoxine hydrochloride and 3- indoles
The mass ratio of acetic acid is the ﹕ 3 of 100 ﹕ 6, and root water sprayed once every 12 days, continuously sprays 5, and humid control is in 70- in domestication grid
85%.Keep bark moist, tamed 60 days in domestication grid；
F. the sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, selected close to water source, it is frostless
Foggy, at the larger forest land of air humidity or cliff, the canopy density in forest land are 55-65%, by hanging or bundling or following closely nail
Etc. fixed form, tree is colonized in above or on cliff, and spray or watering system are set, often spray or watering, holding humidity.
With embodiment one, pseudo-wild cultivating survival rate is 97.5% for the present embodiment final-period management.
Using the candidum tissue culturing seedling of this method pseudo-wild cultivating, land area of one unit yield can effectively improve yield 5-
10 times, pseudo-wild cultivating survival rate is that can effectively reach more than 96.5%.Income can be produced then after transplanting, can be direct
Clip stem of noble dendrobium branch, preferably in Second Year or the 3rd year clip stem of noble dendrobium branch, can its drug effect of effective guarantee value and economic value,
Also stem of noble dendrobium flower can be won in stem of noble dendrobium full-bloom stage and is used as product.
Claims (8)
1. a kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps：
Select seedling：Select and cultivated at least 60 days in culturing room, and the astigmatism hardening candidum tissue culturing seedling of at least 30 days in greenhouse；
Dry in the air seedling：Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby；
Cut sheet material：Selection skin depth, the tree lumber for having lobe ditch, washiness, without grease and gel, such as China fir, Qing Gangshu, pine
The belt leather timber such as tree, willow, wide 10-30cm, long 20-50cm, center thickness 3-10cm belt leather sheet material are cut into, it is standby；
Plant seedlings：Divide candidum tissue culturing seedling obtained by step B to clump, the belt leather side of sheet material obtained by step C be laid in by point, often from or
More from for a bit, and in covering moss on every bit, bundled using the rope made of hemp and be fixed on above-mentioned moss and candidum tissue culturing seedling
On the sheet material, it is desirable to elastic moderate；
Determine root：Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, keeps bark moist, in tame and docile
Change domestication more than 10 days in grid；
The sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, and spray is set or pours water system
System, often spray or watering, keep humidity.
2. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that：In the step D, iron
Spacing of the skin dendrobe tissue culture seedling on panel length direction is 8-12cm.
3. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that：In the step D, fiber crops
Rope is on each point in its middle and lower part Chan Ya 2-4 roads.
4. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that：In the step E, institute
State root water to spray every 7-12 days once, continuously spray 2-5, humid control is in 70-85% in domestication grid.
5. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that：It is fixed in the step F
Root growth has the sheet material of candidum tissue culturing seedling by hanging or bundling or following closely the fixed forms such as nail, is colonized in and sets upper or cliff
On.
6. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that：The root water is salt
Allithiamine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water.
7. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 6, it is characterised in that：The thiamine hydrochloride
Element, the mass ratio of puridoxine hydrochloride and 3-indolyl acetic acid are（80-100）﹕（4-6）﹕（1-3）.
8. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that：The closing in the forest land
Spend for 55-65%.
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