CN107509615A - A kind of candidum tissue culturing seedling pseudo-wild cultivating method - Google Patents

A kind of candidum tissue culturing seedling pseudo-wild cultivating method Download PDF

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Publication number
CN107509615A
CN107509615A CN201710619725.2A CN201710619725A CN107509615A CN 107509615 A CN107509615 A CN 107509615A CN 201710619725 A CN201710619725 A CN 201710619725A CN 107509615 A CN107509615 A CN 107509615A
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seedling
tissue culturing
candidum tissue
pseudo
culturing seedling
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王高平
许东东
陈绍军
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Henan United Source Of Biological Technology Co Ltd
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Henan United Source Of Biological Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/781,3-Thiazoles; Hydrogenated 1,3-thiazoles

Abstract

The invention discloses a kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprise the following steps:Seedling, the seedling that dries in the air are selected, sheet material is cut, plants seedlings, determining root, transplanting.Dried in the air after seedling using seedling, cleaning is selected, directly field planting is bundled on the sheet material with bark of well cutting, then concentrate to be suspended in domestication greenhouse and tame, after candidum tissue culturing seedling determines root, the candidum tissue culturing seedling tamed and belt leather sheet material are transplanted under suitable wild environment together and carry out imitating wild growth.Eliminate tissue-cultured seedling in conventional method and have to pass through greenhouse seedbed and tame this link, reduce production process, save substantial amounts of human and material resources and financial resources input, greatly reduce production cost, shorten the production time;The survival rate of pseudo-wild cultivating is high, and transplantable scope is wide, and its method is simple, convenient, workable, and security reliability is good, and survival rate is high, and later stage phenomenon of filling the gaps with seedlings greatly is reduced.

Description

A kind of candidum tissue culturing seedling pseudo-wild cultivating method
Technical field
The present invention relates to the imitating wildness cultural technique field of dendrobium candidum, and in particular to a kind of candidum tissue culturing seedling is imitated Wild cultivating method.
Background technology
Dendrobium candidum is rare rare traditional Chinese medicine, is known as " a thousand pieces of gold grass ", " plant gold ", the title of " healthy soft gold ", because Its is divinely efficacious, extremely rare, living environment is special and distinguished nourishing effects and rank first of " Chinese nine big celestial grass ", by state Border medicinal plant circle is referred to as " medicine circle giant panda ".Particularly nourishing Yin and moistening lung, nourishing the stomach to improve the production of body fluid, brain nourishing and eyesight improving, the consumptive disease of tonifying five zang organs;It is right Excessive drinking and smoking, overwork, night life excessively, with eye is overstrain one's nerves, vocal cords are hoarse etc. has special efficacy;It is thin to the lung cancer of people Born of the same parents, atrophic gastritis, diabetes have great inhibitory action, and inhibiting rate is up to 74.7%~97.2%, and can be effectively reduced Blood glucose, a new therapy approach is opened for numerous diabetics, is paid attention to by medical field!
However, dendrobium candidum because it is magical with unique the effect of, at home and abroad supply falls short of demand in market, for a long time, Ren Menyong This medicine is all to collect wild resource by excavation, and the temptation of juice makes dendrobium officinale be planted as world's Precious, Rare, Endangered Thing, thus by the attention and protection of country.In order to realize the sustainable use of dendrobium candidum resource, scientific research personnel is from 20th century Begin to artificial cultivation technique research work the seventies, but until this century, seed production, tissue cultures and facility cultivation etc. Artificial breeding key technology is just broken through, and rapid popularization and application.At present, on candidum tissue culturing industrial seedling rearing ring Section, technology is gradually mature and stable, because dendrobium candidum is carried out in artificial environment in tissue culture propagation, its medicinal valency Value is far away from wild dendrobium candidum, therefore necessary use imitates wild method plantation dendrobium candidum, but for iron sheet stone Dry measure used in former times pseudo-wild cultivating method, various technological deficiencies and drawback also be present mostly at present.
Publication No. 105960973A patent of invention discloses a kind of Dendrobidium huoshanness natural as disclosed in September in 2016 28 days The cultural method of sylvan life, it is disadvantageous in that:Need just carry out grafting in annual May, fair weather, by branch from Cut with trunk junction, and the otch progress grafting of a triangle is cut at trunk;It is public disclosed in 4 days January in 2017 The number of opening is that 106258885A patent of invention discloses a kind of dendrobium candidum pseudo-wild cultivating method, is that the stem of noble dendrobium is fixed on 1 year More than mao bamboon grown nonparasitically upon another plant as dendrobium candidum the carrier of cultivation, the lunar calendar October, dendrobium candidum seedling is fixed on to the bamboo of mao bamboon Section top;Publication No. 106376443A patent of invention disclosed in 8 days discloses a kind of imitative open country of dendrobium candidum within 2 months 2017 Agricultural climate condition for corn method, it selects to erect wood to be withered, stem of noble dendrobium directly transplanting is fixed on setting wood, and every layer plants 10 young plants, along standing Wooden ring-type is averagely arranged, and is carried out artificially applying fertilizer management, is simply copied wild Dendrodium growth part feature, be actually field planting, And it is directly to be planted seedlings in the wild with above-mentioned two situations, by natural environment influence, time restriction of planting seedlings is larger, and And survival rate is low.
Above-mentioned various methods have disadvantages in that jointly:1)Program is numerous and diverse, and operation inconvenience, efficiency is low, special Carry out bundling tree under conditions of unfavorable in the wild(Such as height of tree, slope are steep, cliff), operate and implement difficult, It is time consuming;2)Survival rate is low, and cost is high, directly carries out setting in grafting or binding in the wild, the death rate is high, slow-growing, stone Dry measure used in former times seedling wastes seriously, and follow-up workload of filling the gaps with seedlings is big, serious to increase human and material resources and financial resources cost;3)Pseudo-wild cultivating field Institute's limitation is big, and not every trees or cliff are suitable for, such as the trees with grease or gel, smooth-skined tree Wood or cliff etc., conventional method transplanting is unsuitable for, is not easy to wide popularization and application.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of easy, quick, workable, cost is low, survival rate is high, Securely and reliably, the pseudo-wild cultivating method of the candidum tissue culturing seedling of wide adaptation range, requires no candidum tissue culturing seedling This link is tamed with regard to that can rapidly adapt to natural environment in greenhouse seedbed, and when being transferred in wild environment, without moving root, root Fasten admittedly, survival rate is greatly improved, it is ensured that the security and operability of dendrobium candidum pseudo-wild cultivating.
In order to solve the above technical problems, the present invention is adopted the following technical scheme that to realize:
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps:
A. seedling is selected:Select and cultivated at least 60 days in culturing room, and the astigmatism hardening candidum tissue culturing of at least 30 days in greenhouse Seedling, preferably more than plant height 5cm, there is 4-6 strains section, the root of more than 3, stalk is sturdy, leaf color is dark green seedling more preferably;
B. dry in the air seedling:Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby;
C. sheet material is cut:Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu, The belt leather timber such as pine tree, willow, wide 10-30cm, long 20-50cm, center thickness 3-10cm belt leather sheet material are cut into, it is standby;
D. plant seedlings:Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 2-6 strain, step C is laid in from for any per 2-4 The belt leather side of gained sheet material, and in covering moss on every bit, bundled using the rope made of hemp by above-mentioned moss and candidum tissue culturing seedling It is fixed on the sheet material, it is desirable to elastic moderate;
E. root is determined:Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, keeps bark moist, in Tame domestication more than 10 days in grid;
F. transplant:The sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, and set spray or Watering system, often watering, keeps humidity.
In the step D, spacing of the candidum tissue culturing seedling on panel length direction is 8-12cm.
In the step D, the rope made of hemp is on each point in its middle and lower part Chan Ya 2-4 roads.
In the step E, the root water sprayed once every 7-12 days, continuously sprayed 2-5, tamed humid control in grid In 70-85%.
In the step F, determining root growth has the sheet material of candidum tissue culturing seedling solid by hanging or bundling or following closely nail etc. Determine mode, be colonized in tree above or on cliff.
The root water is the mixed aqueous solution of thiamine hydrochloride, puridoxine hydrochloride, 3-indolyl acetic acid and water.
The mass ratio of the thiamine hydrochloride, puridoxine hydrochloride and 3-indolyl acetic acid is(80-100)﹕(4-6)﹕(1-3).
The canopy density in the forest land are 55-65%.
The present invention is relative to the positive beneficial effect acquired by prior art:
The candidum tissue culturing seedling pseudo-wild cultivating method of the application, using selecting seedling, cleaning after seedling of drying in the air, directly field planting, which is bundled in, cuts On the sheet material with bark cut, then concentrate to be suspended in domestication greenhouse and tame, will after candidum tissue culturing seedling determines root The candidum tissue culturing seedling and belt leather sheet material tamed are transplanted under suitable wild environment together to carry out imitating wild growth.It is first First, eliminate tissue-cultured seedling in conventional method and have to pass through greenhouse seedbed and tame this link, reduce production process, save big Human and material resources and the financial resources input of amount, greatly reduce production cost, shorten the production time;Second, pass through the China fir in belt leather The direct domestication of candidum tissue culturing seedling and transplanting, one side production efficiency are significantly improved on tree plate, on the other hand, avoided more The fracture of candidum tissue culturing seedling root system and damage caused by secondary transplanting, stable beneficial to candidum tissue culturing seedling grow, greatly Improve the survival rate of pseudo-wild cultivating;3rd, the present invention, to the direct domestication of candidum tissue culturing seedling, tames and dociles on the sheet material of belt leather After the completion of change, the transplantable more other conventional methods of scope are broad, under conditions of environment is suitable, will carry dendrobium candidum seedling Sheet material, by hanging, bundling or following closely the fixed forms such as nail, be colonized in that tree is upper or cliff on, which greatly enhances trees and The utilization rate in space, increase the production yields of unit area, at the same time it can also prevent ground pest and disease damage.
The root water of the application uses the mixed aqueous solution of thiamine hydrochloride, puridoxine hydrochloride and 3-indolyl acetic acid, and it is rich Containing B family vitamin, stem, leaf development quality and the growth coefficient of candidum tissue culturing seedling are remarkably improved, there is effective powerful It cell-activating function, can penetrate into plant rapidly, promote the plasm of cell to flow, improve cell viability, promote life Long development, the survival rate and growth quality of tissue-cultured seedling are significantly improved, wherein, heteroauxin can also effectively facilitate dendrobium candidum fibrous root Growth.
The inventive method is simple, convenient, workable, and security reliability is good, and survival rate is high, both improves production efficiency Reduce production cost again, later stage phenomenon of filling the gaps with seedlings greatly is reduced, and has preferable economic benefit, social benefit and Ecological Effect Benefit, should be widely promoted application.
Brief description of the drawings
The present invention is described further below in conjunction with the accompanying drawings:
Fig. 1 is the sheet material schematic diagram that the present invention is bundled with candidum tissue culturing seedling;
Fig. 2 is the integrated operation schematic flow sheet of the present invention;
1. ropes made of hemp, 2. sheet material xylems, 3. sheet material barks, 4. mosses, 5. candidum tissue culturing seedlings, 6. fixed mechanisms in figure.
Embodiment
Referring to Fig. 1 and 2, technical solution of the present invention is further elaborated on below in conjunction with accompanying drawing.
Embodiment one
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps:
A. seedling is selected:Select and cultivated 60 days in culturing room, and astigmatism hardening 30 days in greenhouse, more than bottle outlet tissue-cultured seedling plant height 5cm, With 4-6 strain sections, the root of more than 3, stalk is sturdy, the dark green candidum tissue culturing seedling of leaf color;
B. dry in the air seedling:Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby;By the seedling that dries in the air, The hydrophily of candidum tissue culturing seedling root system can be effectively improved, but is attached in its root system on the timber to plant seedlings, root effect is determined in raising Fruit and survival rate;
C. sheet material is cut:Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu, The belt leather timber such as pine tree, willow, wide 10cm, long 20cm, center thickness 3cm belt leather sheet material are cut into, as shown in figure 1, sheet material has There are sheet material xylem 2 and sheet material bark 3, the side of upper board material xylem 2 is provided with fixed mechanism 6, and fixed mechanism 6 is hook or hangs Ring or iron nail, it is standby to prepare sheet material;
D. plant seedlings:Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 2-3 strain, as shown in figure 1, per 3-4 from for some cloth Located at the belt leather side of sheet material obtained by step C, every 3 points are one layer, and the candidum tissue culturing seedling of adjacent two layers is in panel length side Upward spacing is 8-10cm, by control room away from, both be its production retain sufficient space, again be beneficial to effectively improve yield;In Moss 4 is covered on every bit, moss 4 is covered in the root of candidum tissue culturing seedling 5, is bundled using the rope made of hemp 1 by the above-mentioned He of moss 4 Candidum tissue culturing seedling 5 is fixed on the sheet material, and the rope made of hemp 4 twines pressure 2 on each point in its middle and lower part, on the one hand can carry The fixed reliability of height, is on the other hand easy to the later stage to store moisture, and beneficial to the growth of tissue-cultured seedling, it is elastic moderate to twine pressure request, with Moss 4 and candidum tissue culturing seedling 5 are compressed, it is dropped, meanwhile, not breaking or damage tissue-cultured seedling and its root system is advisable;
E. root is determined:Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, and root water is hydrochloric acid sulphur Amine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water, the thiamine hydrochloride, puridoxine hydrochloride and 3- indoles The mass ratio of acetic acid is the ﹕ 1 of 80 ﹕ 4, and root water was sprayed once every 7-8 days, continuously sprayed 3 times, tames humid control in grid and exists 70-85%.Keep bark moist, tamed 15 days in domestication grid;
F. the sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, selected close to water source, it is frostless Foggy, at the larger forest land of air humidity or cliff, the canopy density in forest land are 55-65%, by hanging or bundling or following closely nail Etc. fixed form, tree is colonized in above or on cliff, and spray or watering system are set, often spray or watering, holding humidity.
Final-period management points for attention:When in the morning or afternoon, illumination was weaker, often watering, humidity is kept.Meanwhile Pay attention to repairing and subtract branch, adjust the canopy density of the woods.Pay attention to the prevention and control of plant diseases, pest control work, adhere to " can not medication, just not medication, prevention Based on, integrated control " principle, it is necessary to during medication, strict quarantine is taken, typically with Bravo, thiram, methyl Tobe The preventing and treating stem of noble dendrobium such as Tianjin, zineb, carbendazim black rot, anthracnose, leaf blight, gray mold, droop etc., with close up to preventing and treating snail Ox, slug.
The present embodiment pseudo-wild cultivating survival rate is 96.6%.
Embodiment two
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps:
A. seedling is selected:Select and cultivated 65 days in culturing room, and astigmatism hardening 40 days in greenhouse, more than plant height 5cm, there is 4-6 strains Section, the root of more than 3, the candidum tissue culturing seedling that stalk is sturdy, leaf color is dark green
B. dry in the air seedling:Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby;
C. sheet material is cut:Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu, The belt leather timber such as pine tree, willow, wide 20cm, long 30cm, center thickness 6cm belt leather sheet material are cut into, as shown in figure 1, sheet material has There are sheet material xylem 2 and sheet material bark 3, the side of upper board material xylem 2 is provided with fixed mechanism 6, and fixed mechanism 6 is hook or hangs Ring or iron nail, it is standby to prepare sheet material;
D. plant seedlings:Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 3-4 strain, as shown in figure 1, per 2-3 from for some cloth Located at the belt leather side of sheet material obtained by step C, every 4 points are one layer, and the candidum tissue culturing seedling of adjacent two layers is in panel length side Upward spacing is 8-10cm, and in covering moss 4 on every bit, moss 4 covers in the root of candidum tissue culturing seedling 5, used The rope made of hemp 1, which bundlees, is fixed on above-mentioned moss 4 and candidum tissue culturing seedling 5 on the sheet material, and the rope made of hemp 4 is on each point under wherein Portion twines pressure 3;
E. root is determined:Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, and root water is hydrochloric acid sulphur Amine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water, the thiamine hydrochloride, puridoxine hydrochloride and 3- indoles The mass ratio of acetic acid is the ﹕ 2 of 90 ﹕ 5, and root water sprayed once every 10 days, continuously sprays 2, and humid control is in 70- in domestication grid 85%.Keep bark moist, tamed 30 days in domestication grid;
F. the sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, selected close to water source, it is frostless Foggy, at the larger forest land of air humidity or cliff, the canopy density in forest land are 55-65%, by hanging or bundling or following closely nail Etc. fixed form, tree is colonized in above or on cliff, and spray or watering system are set, often spray or watering, holding humidity.
With embodiment one, pseudo-wild cultivating survival rate is 98.7% for the present embodiment final-period management.
Embodiment three
A kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps:
A. seedling is selected:Select and cultivated 70 days in culturing room, and the astigmatism hardening candidum tissue culturing seedling of 50 days in greenhouse;
B. dry in the air seedling:Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby;
C. sheet material is cut:Selection skin depth, have the tree lumber of lobe ditch, washiness, without grease and gel, as China fir, Qing Gangshu, The belt leather timber such as pine tree, willow, wide 30cm, long 50cm, center thickness 10cm belt leather sheet material are cut into, as shown in figure 1, sheet material has There are sheet material xylem 2 and sheet material bark 3, the side of upper board material xylem 2 is provided with fixed mechanism 6, and fixed mechanism 6 is hook or hangs Ring or iron nail, it is standby to prepare sheet material;
D. plant seedlings:Divide candidum tissue culturing seedling obtained by step B to clump by every clump of 5-6 strain, as shown in figure 1, per 2-3 from for some cloth It it is one layer per 4-5 point, the candidum tissue culturing seedling of adjacent two layers is in panel length located at the belt leather side of sheet material obtained by step C Spacing on direction is 10-12cm, and in covering moss 4 on every bit, moss 4 covers in the root of candidum tissue culturing seedling 5, adopted Bundled with the rope made of hemp 1 and above-mentioned moss 4 and candidum tissue culturing seedling 5 are fixed on the sheet material, the rope made of hemp 4 is on each point in wherein Bottom twines pressure 4;
E. root is determined:Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, and root water is hydrochloric acid sulphur Amine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water, the thiamine hydrochloride, puridoxine hydrochloride and 3- indoles The mass ratio of acetic acid is the ﹕ 3 of 100 ﹕ 6, and root water sprayed once every 12 days, continuously sprays 5, and humid control is in 70- in domestication grid 85%.Keep bark moist, tamed 60 days in domestication grid;
F. the sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, selected close to water source, it is frostless Foggy, at the larger forest land of air humidity or cliff, the canopy density in forest land are 55-65%, by hanging or bundling or following closely nail Etc. fixed form, tree is colonized in above or on cliff, and spray or watering system are set, often spray or watering, holding humidity.
With embodiment one, pseudo-wild cultivating survival rate is 97.5% for the present embodiment final-period management.
Using the candidum tissue culturing seedling of this method pseudo-wild cultivating, land area of one unit yield can effectively improve yield 5- 10 times, pseudo-wild cultivating survival rate is that can effectively reach more than 96.5%.Income can be produced then after transplanting, can be direct Clip stem of noble dendrobium branch, preferably in Second Year or the 3rd year clip stem of noble dendrobium branch, can its drug effect of effective guarantee value and economic value, Also stem of noble dendrobium flower can be won in stem of noble dendrobium full-bloom stage and is used as product.

Claims (8)

1. a kind of candidum tissue culturing seedling pseudo-wild cultivating method, comprises the following steps:
Select seedling:Select and cultivated at least 60 days in culturing room, and the astigmatism hardening candidum tissue culturing seedling of at least 30 days in greenhouse;
Dry in the air seedling:Turn white being dried in the air after candidum tissue culturing seedling cleaning selected by step A under astigmatism to root, it is standby;
Cut sheet material:Selection skin depth, the tree lumber for having lobe ditch, washiness, without grease and gel, such as China fir, Qing Gangshu, pine The belt leather timber such as tree, willow, wide 10-30cm, long 20-50cm, center thickness 3-10cm belt leather sheet material are cut into, it is standby;
Plant seedlings:Divide candidum tissue culturing seedling obtained by step B to clump, the belt leather side of sheet material obtained by step C be laid in by point, often from or More from for a bit, and in covering moss on every bit, bundled using the rope made of hemp and be fixed on above-mentioned moss and candidum tissue culturing seedling On the sheet material, it is desirable to elastic moderate;
Determine root:Plate material lamination after step D is planted seedlings is hung in domestication canopy, and sprays root water, keeps bark moist, in tame and docile Change domestication more than 10 days in grid;
The sheet material determined root growth and have candidum tissue culturing seedling is moved at field forest land or cliff, and spray is set or pours water system System, often spray or watering, keep humidity.
2. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that:In the step D, iron Spacing of the skin dendrobe tissue culture seedling on panel length direction is 8-12cm.
3. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that:In the step D, fiber crops Rope is on each point in its middle and lower part Chan Ya 2-4 roads.
4. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that:In the step E, institute State root water to spray every 7-12 days once, continuously spray 2-5, humid control is in 70-85% in domestication grid.
5. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that:It is fixed in the step F Root growth has the sheet material of candidum tissue culturing seedling by hanging or bundling or following closely the fixed forms such as nail, is colonized in and sets upper or cliff On.
6. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that:The root water is salt Allithiamine element, puridoxine hydrochloride, the mixed aqueous solution of 3-indolyl acetic acid and water.
7. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 6, it is characterised in that:The thiamine hydrochloride Element, the mass ratio of puridoxine hydrochloride and 3-indolyl acetic acid are(80-100)﹕(4-6)﹕(1-3).
8. candidum tissue culturing seedling pseudo-wild cultivating method as claimed in claim 1, it is characterised in that:The closing in the forest land Spend for 55-65%.
CN201710619725.2A 2017-07-26 2017-07-26 A kind of candidum tissue culturing seedling pseudo-wild cultivating method Pending CN107509615A (en)

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Cited By (6)

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CN104115733A (en) * 2014-08-07 2014-10-29 深圳市仙湖植物园管理处 Under-forest three-dimensional cultivation method for Dendrobium officinale
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CN110122089A (en) * 2018-02-08 2019-08-16 苏庭照 A kind of implantation methods of dendrobium candidum
CN109511535A (en) * 2018-12-20 2019-03-26 广西壮族自治区农业科学院生物技术研究所 A kind of dendrobium candidum bionic cultivation method
CN109511535B (en) * 2018-12-20 2021-05-14 广西壮族自治区农业科学院生物技术研究所 Bionic cultivation method for dendrobium officinale
CN110521528A (en) * 2019-09-26 2019-12-03 广西壮族自治区林业科学研究院 A kind of bionical implantation methods of scenic spot hayashishita orchid
CN110771489A (en) * 2019-11-26 2020-02-11 浙江省亚热带作物研究所 Dendrobium officinale cultivation device and cultivation method
CN111328692A (en) * 2020-04-08 2020-06-26 安徽霍山麟鼎轩生物科技有限公司 Natural wild-imitating planting method for dendrobium huoshanense
CN112056197A (en) * 2020-09-07 2020-12-11 贵州沿河乌江生物科技发展有限公司 Rapid tree culture method for dendrobium officinale tissue culture seedlings

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Application publication date: 20171226