CN109425700A - A method of test diversiform-leaved poplar seed sprouts ability and matrix salt content relationship - Google Patents
A method of test diversiform-leaved poplar seed sprouts ability and matrix salt content relationship Download PDFInfo
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Abstract
The present invention relates to a kind of methods that test diversiform-leaved poplar seed sprouts ability and matrix salt content relationship, including building diversiform-leaved poplar cultivating seeds model；Capacity data is sprouted to obtain；The data acquisition of matrix salt content；Growth rate and matrix salt content data building both sides relation curve in the period are being sprouted according to diversiform-leaved poplar seed.A kind of test diversiform-leaved poplar seed provided by the invention is sprouted in the method for ability and matrix salt content relationship, diversiform-leaved poplar seed is calculated by acquisition diversiform-leaved poplar seed time three-dimensional model diagram is sprouting the growth rate in the period, it is capable of the growth rate of precise measurement diversiform-leaved poplar seed in this way, reduces diversiform-leaved poplar seed in the error for sprouting relation curve between growth rate and matrix salt content data in the period；Areal calculation mode is projected in tri- axis direction of XYZ on tri- faces of apex coordinate calculation and XYZ in addition, having used when growth rate of the diversiform-leaved poplar seed within the sprouting period is calculated, final error can be reduced to the greatest extent.
Description
Technical field
The present invention relates to diversiform-leaved poplar seed testing fields, sprout ability and base in particular to a kind of test diversiform-leaved poplar seed
The method of matter salt content relationship.
Background technique
Diversiform-leaved poplar is distributed in desert, half-desert area, and ecological environment is extremely harsh.Why diversiform-leaved poplar can be extremely harsh
It survives under ecological environmental condition, and a kind of ancient and long-lived rare tree species can be become, with the distinctive biology of own, ecology
Learning characteristic has close relationship.
Diversiform-leaved poplar blade profile is changeable, bears Cheng Qiguan.When you come into Populus Euphratica, the leaf different of it is seen first.In sub- seedling children
On the coppice shoot of tree or bearing tree base portion, leaf is in lanceolar, long and narrow such as willow；And the leaf on adult branch, shape have ovum
Shape, diamond shape, heart-shaped, semicircle and triangle etc., it is quite inconsistent.It is this to grow different shape leaf in different developmental phases
Phenomenon is a kind of diversiform-leaved poplar biological characteristics most outstanding.This different Ye Xianxiang is known as " different blade profile of evolving " in botany.Just
It is the leaf form changed because of there are many diversiform-leaved poplars, diversiform-leaved poplar is referred to as " cotton tree " again by usual people.Also it is believed that,
Five kinds of leaves that diversiform-leaved poplar has shape of different sizes are set at one plant, thus say jokingly its " Wu Tongshu ".Whenever it is seen that one plant
When leaf there are many diversiform-leaved poplar tree is upper, all feel rare and exclaim.In fact, the keratin of the heterophyll and leaf of diversiform-leaved poplar, is conducive to subtract
Few internal salinity evaporation and consumption, are a kind of adaptability to drought environment.
Diversiform-leaved poplar is not xerophilous tree species, but there is stronger drought-resistant ability.It is grown in the plant in arid desert area, cannot rely on
Atmospheric precipitation existence, salinity source needed for they grow, mainly underground water and stream runoff water and condensing from air are obtained
The soil water obtained.So desert belt plant must all have huge horizontal root system and strong osmotic pressure.Meanwhile
Their aerial part, it is also necessary to have the raw morphosis of drought, to reduce salinity evaporation, to adjust intracorporal salt balance.
Diversiform-leaved poplar is not afraid of bubble, is in long-term ponding in lake basin, continues five months, remain to survive.Winter ponding climbs ice four or five months, diversiform-leaved poplar
It will not freeze to death.If diversiform-leaved poplar grows in argillic horizon, soil moisture content is up to 20% or more, its root will not rot.
This explanation, diversiform-leaved poplar not only has very strong water resistance, and has very strong erosion resistance, especially after bubble, anti-corruption
Property is stronger.
Diversiform-leaved poplar fruit mature period is long, wants that plucking fruit must bear with.Diversiform-leaved poplar is dioecism, and first Hua Houye tree species, single plant is grown to
It can yield positive results at 12 years old.Under normal circumstances, diversiform-leaved poplar blooms at the beginning of late April to 5 months, and the florescence 15 days or so；And fruit at
The ripe phase starts from late June, can extend to late August always, blooms to fructescence up to more than 120 days, is fruit in Salicaceae
Ripe phase longest tree species.Annual seven, the August, a large amount of seeds danced in the air all over the sky with white pappus when diversiform-leaved poplar fruit mature period arrives,
It floats with fair wind several hundred kilometers, exactly likes midsummer snowfall, become one Greater Landscape of nature.Diversiform-leaved poplar although ancient longevity, but its seed
But very little, mass of 1000 kernel only have 0.08-0.2 grams, every gram of seed amount up to more than 12000, very rare.Diversiform-leaved poplar seed is small,
Under natural conditions, germinating capacity can only be kept more than 30 days.Therefore, test diversiform-leaved poplar seed sprouts ability most under the conditions of what salinity
Surging current urgent problem.
In view of this, the present invention is specifically proposed.
Summary of the invention
The purpose of the present invention is to provide it is a kind of test diversiform-leaved poplar seed sprout ability and matrix salt content relationship method,
The relationship of diversiform-leaved poplar seed Yu matrix salt content is measured by what this method was capable of accurate difference, and excludes salinity, micro-
Influence of the factors such as secondary element, temperature, humidity, illumination to test process.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A method of test diversiform-leaved poplar seed sprouts ability and matrix salt content relationship, comprising the following steps:
Diversiform-leaved poplar seed treatment: diversiform-leaved poplar seed is impregnated into 10min-120min, the plant nutrition liquid in plant nutrition liquid
Including diversiform-leaved poplar nursery stock extracting solution；Also carry out ultrasonication simultaneously in soaking process, the power of ultrasonic wave is 100-200W, often
Secondary ultrasound 5-8s, it is adjacent twice ultrasound between be divided into 20-30s；
It constructs diversiform-leaved poplar cultivating seeds model: diversiform-leaved poplar seed being pressed into circular arrangement in culture medium, soil is added in culture medium
Modifying agent, component includes: the soil conditioner raw material in parts by weight
100-150 parts of diatomite, 80-120 parts of flyash, 70-90 parts of bentonite, 50-70 parts of kaolin, turf 80-120
Part, 120-160 parts of plant straw powder, -20 parts of carrier 12 containing algae, 40-60 parts of organic fertilizer, 40-70 parts of humus, zeolite powder
20-35 parts, 20-30 parts of glass microballoon, 18-25 parts of Cab-O-sil, 3-5 parts of hydrophobic resin, 20-30 parts of microbial inoculum；
10-20 parts of POLYPROPYLENE GLYCOL, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of waste residue of Chinese herbs powder, useless tea grounds
20-40 parts, 8-24 parts of fulvic acid concentrate, 40-60 parts of vinasse, 30-60 parts of leaf mould, 40-60 parts of sawdust, rice chaff ash 20-30
Part, loose 10-30 parts of phosphorus, 50-70 parts of coco bran and 30-40 parts of slow-release fertilizer；
It sprouts capacity data to obtain: being sprouted in the period in diversiform-leaved poplar seed, diversiform-leaved poplar seed to be measured is obtained by spatial digitizer
Cultivate the time three-dimensional model diagram of model；The time three-dimensional model diagram includes several diversiform-leaved poplar cultivating seeds models to be measured in difference
Moment corresponding three-dimensional model diagram；It is analyzed and is handled by time three-dimensional model diagram, and then the Hu is calculated
Diversiform-leaved poplar seed is sprouting the growth rate in the period in poplar cultivating seeds model；
The data acquisition of matrix salt content: it is sprouted in the period in diversiform-leaved poplar seed, acquires the diversiform-leaved poplar cultivating seeds to be measured
Culture substrate sample in model around diversiform-leaved poplar seed；The culture substrate sample around diversiform-leaved poplar seed is measured by salinity tester
Then matrix salt is calculated by salt content data in the culture substrate sample around diversiform-leaved poplar seed in middle salt content data
Divide content data；
Growth rate and matrix salt content building both sides relation curve in the period are being sprouted according to diversiform-leaved poplar seed.
Inventor herein has been surprisingly found that, diversiform-leaved poplar seed is impregnated in plant nutrition liquid, and the fecundity of diversiform-leaved poplar seed becomes
By force, it so that the survival rate of diversiform-leaved poplar seed is promoted, reduces diversiform-leaved poplar seed and contains in the growth rate sprouted in the period with matrix salinity
Measure the error of both sides relation curve.
Secondly, diversiform-leaved poplar nursery stock extracting solution is added in plant nutrition liquid, so that diversiform-leaved poplar seed absorbs recklessly in advance before sprouting
The juice of Yang Miaomu can make the survival rate of diversiform-leaved poplar seed be promoted；Volume of the diversiform-leaved poplar nursery stock extracting solution in the culture solution
Percentage is 5%-30%.In addition, finding in test, the diversiform-leaved poplar nursery stock extracting solution for adding excessive content is easy to cause diversiform-leaved poplar seed
It is contaminated, therefore, adds diversiform-leaved poplar nursery stock extracting solution appropriate and not only reached the problem of promoting survival rate well, but also be unlikely to diversiform-leaved poplar
Seed is infected.
In this soil conditioner provided by the invention, the various components with specific compatibility relationship are selected, specifically may be used
To be divided into improvement component and component of fixing the sand.It wherein, is main with POLYPROPYLENE GLYCOL, hydrophobic resin and bentonite in component of fixing the sand
Component, to realize the function fixed to loose soil (sand) matter, the molding layer that fixes the sand of formation, more it is essential that in this application,
Also contain changing with compositions such as kaolin, flyash, diatomite, turf, organic fertilizer, humus, waste residue of Chinese herbs powder and useless tea grounds
Good component.These components are especially advantageous for the environment of diversiform-leaved poplar seed growth according to that can be formed after set compatibility relationship proportion；And
The environment is also especially advantageous for rapid microbial growth metabolism (fulvic acid concentrate, humus play main effect)；Fulvic acid is dense
Contracting liquid and humus collective effect can be used for improving diversiform-leaved poplar seed microenvironment；Promote substance during diversiform-leaved poplar seed growth and
The conversion of energy, so that diversiform-leaved poplar seed be made to show healthy situation；Simultaneously, moreover it is possible to inhibit the growth of harmful fungoid, and supply
Nutrient needed for diversiform-leaved poplar seed growth；Action temperature and, it is biodegradable.It should be strongly noted that initiative is low to match in the application
5 with carrier containing algae, glass microballoon, Cab-O-sil, microbial inoculum, fulvic acid concentrate, useless tea grounds, waste residue of Chinese herbs powder, rice chaff
The improvement component of the compositions such as ash, after several component mix and match, it has unexpectedly been found that, the combination is for matrix where diversiform-leaved poplar seed
The raising of the water-retaining property and gas permeability of soil has the low raising of highly significant, improves the survival rate of diversiform-leaved poplar seed.
In addition, the plant straw powder includes following component by weight percentage:
Milled powders of cornstalk 8-20%, maize cob meal 6-20%, potato straw powder 9-25%, sea-buckthorn straw powder 10-30%, camel
Pierce straw powder 10-15%, rice straw powder 10-15%, flax straw powder 8-15%, wheat stalk powder 5-15%, broomcorn straw
Powder 5-20% and pea straw powder 5-20%.
The microbial inoculum is by one of actinomyces, nitrogen-fixing bacteria, bacillus subtilis, photosynthetic bacteria, saccharomycete
Or the bacterium solution after a variety of cultivation and fermentations.
After complex microorganism bacterium solution and the effect of organic biofertilizer and humic acid, a series of metabolites can be generated, are led to
It crosses the competition to space and nutrition and generates a variety of antibacterial pest-resistant substances, play the pest-resistant purpose of diseases prevention.In addition, can also stimulate
The growth and development of diversiform-leaved poplar seed improves crop anti-adversity, promotes the development of diversiform-leaved poplar seed growth.
Vermiculite power has good cationic exchangeability, adsorptivity, water-retaining property, improves root system surface microenvironment, water storage is saturating
Gas；The pH value of root system ambient enviroment can be buffered, keeps nutrient slowly-released, mineral element is provided, promote diversiform-leaved poplar seed growth.
Fixative plays filming function, other each components is enabled smoothly to be coated on diversiform-leaved poplar the surface of the seed.According to upper
The weight ratio stated mixes and manufactured soil conditioner, while each component plays respective effect, can also between different components
Synergistic effect is generated, it, can be so that coating agent generates the Related Components such as antibacterial, insect prevention, organic-inorganic nutrient, positive growth factor
It realizes and diversiform-leaved poplar seed is promoted to sprout, improve the survival rate of diversiform-leaved poplar seed.More importantly each component of the coating agent is
Pure natural component, it is biodegradable, it is non-hazardous to people and animals and environment, meet the non-harmful requirement of agricultural production.
The carrier containing algae, which is negative, is loaded with total ball algae, false ball algae, column silk algae, Scytonema arcangelii, sheath silk algae, micro- sheath algae, fish raw meat altogether
Two or more macromolecule polymeric material in algae, slimeball algae and nostoc.
There is stronger breeding energy in view of total ball algae, false ball algae, column silk algae, Scytonema arcangelii, sheath silk algae and micro- sheath algae etc. altogether
Power and resistance ability, meanwhile, mostly in lichens parasymbiosis, and the combination of several algae can also embody the effect of synergy
Fruit.It is therefore preferable that using above-mentioned several algae as breeding kind.In addition, carrier containing algae preferably has preferable water holding capacity,
And readily flowed macromolecule polymeric material, it is intended to improve the survival ability and propagation performance of algae.
Preferably, the soil conditioner is made by the following steps:
1), kaolin and flyash are uniformly mixed, and are added that water stirring is agglomerating, and dough is placed under the conditions of 30-35 DEG C
Heating 1-1.5 hours, obtains the first mixture；
2) fulvic acid concentrate is added after, mixing diatomite and turf, adds water and continues to mix, obtain the second mixing
Material；
3) straw decomposing inoculant decomposed rear and organic fertilizer, humus, waste residue of Chinese herbs powder and useless tea, is added in plant straw powder
Slag mixing, and mix after coco bran mixes and mix thoroughly with vinasse, leaf mould, sawdust, rice chaff ash and loose phosphorus after heap fermentation, and in 20-
30 DEG C stand 2-3 days, obtain third mixture；
4) hydrophobic resin is added after, mixing zeolite powder, glass microballoon and Cab-O-sil to stir evenly；Obtain the 4th mixing
Material；
5) urea, monoammonium phosphate and slow-release fertilizer are added after, mixing second mixture, the third mixture, and
Dispersion stirring is carried out, then first mixture and the 4th mixture are added thereto, is stirring evenly and then adding into and is carried containing algae
Body and microbial inoculum continue under paving after mixing to 25-30 DEG C of environment 2-5 hours, obtain pre- using powder；
6) quality is its 2-4 times water and quality is its 1%-4% inorganic salts, are added in bentonite, it is agitated or
Extrusion modification, then be classified and purified, obtain slurries of the granularity 90% less than 10 μm；By the slurries and have been dissolved in water
POLYPROPYLENE GLYCOL aqueous solution, water carry out mixture, and be individually encapsulated after mixing evenly with 20-30 revs/min of revolving speed, obtain pre- use
Liquid material.
In addition, the time of diversiform-leaved poplar seed immersion can be shortened by ultrasonication, further promoted diversiform-leaved poplar seed at
Motility rate.
The diversiform-leaved poplar nursery stock extracting solution has the following steps to be made:
The water of diversiform-leaved poplar nursery stock, 5~10 times of diversiform-leaved poplar seedling qualities is subjected to 1~2h of hyperbaric heating, filtrate and filter is obtained by filtration
Slag；
Filter residue and filtrate are placed in closed container, zymophyte is accessed in a reservoir and carries out 4~6h of fermentation；
Stock extracting solution is obtained by filtration after fermentation.
It is found through overtesting, the stock extracting solution obtained after filtrate and filter residue are fermented can further increase grafting
The survival rate of nursery stock.
Preferably, in the diversiform-leaved poplar cultivating seeds model, adjacent diversiform-leaved poplar seed spacing is no more than 15cm, and in culture medium recklessly
The quantity of poplar seed is no more than 40.
In sprouting capacity data acquisition process, rejects diversiform-leaved poplar seed and sprout ability abnormal data, rejecting abnormalities data master
If to sprout or sprouting abnormal diversiform-leaved poplar seed, the sprouting rate of these diversiform-leaved poplar seeds can be very low or deviates normal model
In enclosing.
Preferably, it is analyzed and is handled by time three-dimensional model diagram, and then the diversiform-leaved poplar cultivating seeds are calculated
Diversiform-leaved poplar seed is sprouting the growth rate in the period in model, comprising:
The three-dimensional coordinate of acquisition time three-dimensional model diagram obtains apex coordinate in tri- axis direction of XYZ；
According to apex coordinate, diversiform-leaved poplar seed is calculated and is sprouting the growth rate in the period:
。
Preferably, it is analyzed and is handled by time three-dimensional model diagram, and then the diversiform-leaved poplar cultivating seeds are calculated
Diversiform-leaved poplar seed is sprouting the growth rate in the period in model, comprising:
The three-dimensional coordinate of acquisition time three-dimensional model diagram, and then obtain three-dimensional model diagram and projected in tri- axis directions of XYZ
Area；
According to projected area in tri- axis directions of XYZ, diversiform-leaved poplar seed is calculated and is sprouting the growth rate in the period:
。
Preferably, the acquisition position of the culture substrate sample be the upper end of diversiform-leaved poplar seed centre coordinate, lower end, left end,
Right end, front end, rear end.
Preferably, the matrix salt content data are calculated by following equation:
Ct=KOn*Wt On+KUnder*Wt Under+KIt is left*Wt It is left+KIt is right*Wt It is right+KBefore*Wt Before+KAfterwards*Wt Afterwards。
Preferably, both growth rate and the building of matrix salt content data in the period are being sprouted according to diversiform-leaved poplar seed
After relation curve, salinity calibration model is constructed:
It is sprouted in the period in diversiform-leaved poplar seed, acquires upper end, lower end, left end, the right side of diversiform-leaved poplar seed neighboring seeds centre coordinate
The culture substrate sample at end, front end, rear end measures salt content in the culture substrate sample of neighboring seeds by salinity tester
Then salinity correction coefficient is calculated in data；
Growth rate and matrix salt content relationship song in the period are being sprouted to diversiform-leaved poplar seed according to salinity correction coefficient
Line is modified.
Preferably, in the calculation formula of the matrix salt content data:
。
Preferably, the measurement for sprouting capacity data is on the model established in 3-matic Top-Down Design software
It carries out.
Preferably, both growth rate and the building of matrix salt content data in the period are being sprouted according to diversiform-leaved poplar seed
Temperature correction model and moisture calibration model are constructed after relation curve, are obtained by temperature correction model and moisture calibration model
Temperature correction facotor and moisture correction coefficient；
Growth rate and matrix in the period are being sprouted to diversiform-leaved poplar seed according to temperature correction facotor and moisture correction coefficient
Salt content relation curve is modified.
Compared with prior art, the invention has the benefit that
1, a kind of test diversiform-leaved poplar seed provided by the invention sprouts the method for ability and matrix salt content relationship, using circle
The diversiform-leaved poplar cultivating seeds model of shape can guarantee the building that more set of model are realized in a culture medium, and in the same training
Support base in can guarantee as far as possible nurturing an environment be it is identical, reduce diversiform-leaved poplar seed sprout the period in growth rate and base
The error of relation curve between matter salt content data.
2, a kind of test diversiform-leaved poplar seed provided by the invention is sprouted in the method for ability and matrix salt content relationship, is passed through
Acquisition diversiform-leaved poplar seed time three-dimensional model diagram is sprouting the growth rate in the period to calculate diversiform-leaved poplar seed, can accurately survey in this way
The growth rate for measuring diversiform-leaved poplar seed reduces diversiform-leaved poplar seed and is sprouting the growth rate in the period and closing between matrix salt content
It is the error of curve；In addition, having used tri- axis of XYZ when growth rate of the diversiform-leaved poplar seed within the sprouting period is calculated
Areal calculation mode is projected on direction in tri- axis directions of apex coordinate calculation and XYZ, can be reduced to the greatest extent most
Whole error.
3, a kind of test diversiform-leaved poplar seed provided by the invention is sprouted in the method for ability and matrix salt content relationship, is passed through
Acquire the culture substrate sample in the diversiform-leaved poplar cultivating seeds model to be measured around diversiform-leaved poplar seed, especially culture substrate sample
Acquisition position is upper end, lower end, left end, right end, front end, the rear end of diversiform-leaved poplar seed centre coordinate, and matrix salt is finally calculated
Point content, can reduce diversiform-leaved poplar seed sprout the growth rate in the period and between matrix salt content relation curve mistake
Difference.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
The embodiment of the present invention provides the method for a kind of test diversiform-leaved poplar seed sprouting ability and matrix salt content relationship, including
Following steps:
Diversiform-leaved poplar seed treatment: diversiform-leaved poplar seed is impregnated into 10min-120min, the plant nutrition liquid in plant nutrition liquid
Including diversiform-leaved poplar nursery stock extracting solution；Also carry out ultrasonication simultaneously in soaking process, the power of ultrasonic wave is 100-200W, often
Secondary ultrasound 5-8s, it is adjacent twice ultrasound between be divided into 20-30s；
It constructs diversiform-leaved poplar cultivating seeds model: diversiform-leaved poplar seed being pressed into circular arrangement in culture medium, soil is added in culture medium
Modifying agent, component includes: the soil conditioner raw material in parts by weight
100-150 parts of diatomite, 80-120 parts of flyash, 70-90 parts of bentonite, 50-70 parts of kaolin, turf 80-120
Part, 120-160 parts of plant straw powder, -20 parts of carrier 12 containing algae, 40-60 parts of organic fertilizer, 40-70 parts of humus, zeolite powder
20-35 parts, 20-30 parts of glass microballoon, 18-25 parts of Cab-O-sil, 3-5 parts of hydrophobic resin, 20-30 parts of microbial inoculum；
10-20 parts of POLYPROPYLENE GLYCOL, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of waste residue of Chinese herbs powder, useless tea grounds
20-40 parts, 8-24 parts of fulvic acid concentrate, 40-60 parts of vinasse, 30-60 parts of leaf mould, 40-60 parts of sawdust, rice chaff ash 20-30
Part, loose 10-30 parts of phosphorus, 50-70 parts of coco bran and 30-40 parts of slow-release fertilizer；
It constructs diversiform-leaved poplar cultivating seeds model: diversiform-leaved poplar seed being pressed into circular arrangement in culture medium；It is arranged using circular seed
Mode for cloth, which can to cultivate, to influence each other and minimizes between model, at the same cultivate influence each other between model it is more average；
And during constructing diversiform-leaved poplar cultivating seeds model, in general adjacent diversiform-leaved poplar seed spacing is no more than 15cm, and cultivates
The quantity of diversiform-leaved poplar seed is no more than 40 in base；Firstly, can guarantee to train at one using circular diversiform-leaved poplar cultivating seeds model
Support the building that more set of model are realized in base；Secondly, can guarantee as far as possible in the same culture medium nurturing an environment be it is identical,
Reduce diversiform-leaved poplar seed in the error for sprouting relation curve between growth rate and matrix salt content data in the period.Certainly
The present invention can use the arrangement mode of Else Rule shape, can also use other in building diversiform-leaved poplar seed trining mock up
The arrangement mode of irregular shape, still available diversiform-leaved poplar seed sprouts the method for ability and matrix salt content relationship.But
It is by largely repeatedly experiment discovery, the diversiform-leaved poplar seed arrangement mode of other shapes (including regular shape and irregular shape)
The coefficient of dispersion of relation curve is larger between the growth rate and matrix salt content data of measurement, only the diversiform-leaved poplar of circular arrangement
For coefficient of dispersion close to 1, the result of measurement is more accurate after seed repeatedly measures.
In sprouting capacity data acquisition process, rejects diversiform-leaved poplar seed and sprout ability abnormal data, rejecting abnormalities data master
If to sprout or sprouting abnormal diversiform-leaved poplar seed, the sprouting rate of these diversiform-leaved poplar seeds can be very low or deviates normal model
In enclosing.
Sprout capacity data obtain, the present invention in diversiform-leaved poplar seed sprout the period in growth rate to sprout ability into
Row characterization: sprouting in the period in diversiform-leaved poplar seed, and the time for obtaining diversiform-leaved poplar cultivating seeds model to be measured by spatial digitizer is three-dimensional
Illustraton of model；The time three-dimensional model diagram includes several diversiform-leaved poplar cultivating seeds models to be measured in different moments corresponding three-dimensional
Illustraton of model；It is analyzed and is handled by time three-dimensional model diagram, and then be calculated in the diversiform-leaved poplar cultivating seeds model recklessly
Poplar seed is sprouting the growth rate in the period；
In the present invention, there are two types of embodiment, one is solids apart from difference method, this method tool for the calculating of growth rate
Body process is as follows: being analyzed and is handled by time three-dimensional model diagram, and then the diversiform-leaved poplar cultivating seeds model is calculated
Middle diversiform-leaved poplar seed is sprouting the growth rate in the period, comprising:
The three-dimensional coordinate of acquisition time three-dimensional model diagram obtains some moment subsequent time XYZ corresponding with the moment
Apex coordinate in three axis directions, i.e., it is at a time corresponding in tri- axis direction of subsequent time XYZ corresponding with the moment
Extreme point；Assuming that maximum of points and minimum point in X-axis are respectively (X in t momentt max, y1, z1)、(Xt min, y2, z2)；Y-axis
On maximum of points and minimum point be respectively (x3, Yt max, z3)、(x4, Yt min, z4)；Maximum of points and minimum value on Z axis
Point is respectively (x5, y5, Zt max)、(x6, y6,,, Zt min)；Assuming that maximum of points and minimum point point in t+ △ t moment, in X-axis
It Wei not (Xt+1 max, y1 *, z1 *)、(Xt+1 min, y2 *, z2 *)；Maximum of points and minimum point in Y-axis are respectively (x3 *, Yt+1 max,
z3 *)、(x4 *, Yt+1 min, z4 *)；Maximum of points and minimum point on Z axis are respectively (x5 *, y5 *, Zt+1 max)、(x6 *, y6 * ,,,
Zt+1 min)
According to the apex coordinate of t moment and t+1 moment, diversiform-leaved poplar seed is calculated and is sprouting the growth rate in the period:
In formula 1, VtThe growth rate in the period is being sprouted for diversiform-leaved poplar seed； λ1、λ2、λ3、λ4、λ5、λ6It is range difference side's coefficient, wherein λ1、λ2、λ4、λ5Value range be 0.95~
Between 1.05, λ3Value range between 0.27~0.67, λ6Value range between 1.84~3.89.Because in diversiform-leaved poplar
In the growth course of seed, often top (i.e. in Z-direction) has the faster speed of growth, life of the bottom with respect to other directions
Long speed is more slow, therefore, in order to ensure that the diversiform-leaved poplar seed that obtains that can be relatively accurate is sprouting the growth rate in the period, λ3
Value range it is relatively small, λ6Value range it is relatively large.And λ3/λ6Preferably withIt is inversely proportional.
In the present invention, one is projected area difference method, the specific mistakes of this method for another calculation of growth rate
Journey is as follows: crossing time three-dimensional model diagram and is analyzed and handled, and then diversiform-leaved poplar in the diversiform-leaved poplar cultivating seeds model is calculated
Seed is sprouting the growth rate in the period, comprising:
The three-dimensional coordinate of acquisition time three-dimensional model diagram, and then obtain some moment subsequent time corresponding with the moment
Three-dimensional model diagram projected area in tri- axis directions of XYZ；Assuming that three-dimensional model diagram is respectively in tri- axis directions of XYZ in t moment
On projected area be St X、St Y、St Z；In t+ △ t moment, the three-dimensional model diagram projected area in tri- axis directions of XYZ respectively
For St+1 X、St+1 Y、St+1 Z
According to projected area in tri- axis directions of XYZ, diversiform-leaved poplar seed is calculated and is sprouting the growth rate in the period:
In formula 2, VtThe growth rate in the period is being sprouted for diversiform-leaved poplar seed；μ1、μ2、μ3It is perspective plane product moment side coefficient,
Wherein μ1、μ2Value range between 0.72~0.86, μ3Value range between 0.94~1.17.Because of diversiform-leaved poplar seed
During the growth process, the opposite variation of Z-direction projected area is smaller, and X, Y direction projected area change greatly relatively, therefore
The diversiform-leaved poplar seed that obtains to guarantee relatively accurate is sprouting the growth rate in the period, μ3Value range it is relatively large, μ1、
μ2Value range it is relatively small, and μ1/μ2With (St+1 X-St X)/(St+1 Y-St Y) be inversely proportional.
In the present invention, the measurement for sprouting capacity data is that the model established in 3-matic Top-Down Design software is enterprising
Row.
Matrix salt content data acquisition: it is sprouted in the period in diversiform-leaved poplar seed, acquires the diversiform-leaved poplar cultivating seeds mould to be measured
Culture substrate sample in type around diversiform-leaved poplar seed；For the data that can accurately measure matrix salt content, data essence is improved
Degree, the acquisition position of culture substrate sample are upper end, lower end, left end, right end, front end, the rear end of diversiform-leaved poplar seed centre coordinate；It is false
It is located at t moment, collects the upper end of diversiform-leaved poplar seed centre coordinate, lower end, left end, right end, front end, rear end sample are respectively MOn、
MUnder、MIt is left、MIt is right、MBefore、MAfterwards。
Culture substrate sample M is measured by salinity testerOn、MUnder、MIt is left、MIt is right、MBefore、MAfterwardsMiddle salt content data are respectively
WOn、WUnder、WIt is left、WIt is right、WBefore、WAfterwards, base then is calculated by salt content data in the culture substrate sample around diversiform-leaved poplar seed
Matter salt content data；Matrix salt content is calculated by following equation:
Ct=KOn*Wt On+KUnder*Wt Under+KIt is left*Wt It is left+KIt is right*Wt It is right+KBefore*Wt Before+KAfterwards*Wt Afterwards (3)
In formula 3, CtFor matrix salt content；KOn、KUnder、KIt is left、KIt is right、KBefore、KAfterwardsIt is salt content coefficient；Wherein, KIt is left、KIt is right、
KBefore、KAfterwardsValue range between 0.87~1.12；KOnValue range between 0.24~0.32；KUnderValue range exist
Between 1.37~1.87, wherein KOn、KUnderPreferably meet:
In formula 4, HOnFor culture substrate sample MOnVertical range of the acquisition position apart from stromal surface, HUnderFor culture substrate
Sample MUnderVertical range of the acquisition position apart from stromal surface.Because being sprouted in the period in diversiform-leaved poplar seed, the base of diversiform-leaved poplar seed
Matter salt content referential data needs the salt divided data of the comprehensive each position in seed periphery, and the present invention chooses diversiform-leaved poplar kind subcenter and sits
Target upper end, lower end, left end, right end, front end, rear end sample, these samples have certain representativeness；Further, since seed
The salt divided data of upper end on seed growth rate influence it is smaller, and the salt divided data below seed on seed growth rate influence compared with
Greatly, therefore the present invention willTo improve the accuracy of matrix salt content data.
Growth rate and matrix salt content data building both sides relation song in the period are being sprouted according to diversiform-leaved poplar seed
Line.
In the present invention, calibration model can also be constructed, building calibration model includes in general including very much: salt branch school
Positive model, temperature correction model, moisture calibration model；Above-mentioned calibration model, which can be used alone, to be applied in combination.
Salinity calibration model is introduced first: because diversiform-leaved poplar cultivating seeds model is there are multiple diversiform-leaved poplars during building
Seed is arranged in culture medium, therefore when measuring some diversiform-leaved poplar seed matrix salt content data, diversiform-leaved poplar seed adjacent thereto
Also the salinity of surrounding can be absorbed, therefore the presence of some diversiform-leaved poplar seed matrix salt content DATA REASONING can be caused to a certain extent
Error, therefore salinity correction carries out in the following way:
It is sprouted in the period in diversiform-leaved poplar seed, acquires upper end, lower end, left end, the right side of diversiform-leaved poplar seed neighboring seeds centre coordinate
The culture substrate sample at end, front end, rear end measures salt content in the culture substrate sample of neighboring seeds by salinity tester
Then the matrix salt content C of neighboring seeds can be calculated in data according to formula (3)t 1、Ct 2, according to the matrix of neighboring seeds
Salinity correction coefficient η is calculated in salt content, wherein the calculation formula of η are as follows:
Multiply C with salinity correction coefficienttThen matrix salt content after being corrected is sprouting the period according to diversiform-leaved poplar seed
Matrix salt content data after interior growth rate and correction construct both sides relation curve.
Secondly, introducing temperature correction model: the temperature data of diversiform-leaved poplar seed local environment in the period is sprouted in acquisition, is obtained every
It temperature variation curve；Mean temperature change curve is obtained according to daily temperature variation curve；By daily temperature change
Curve obtains corresponding temperature correction facotor with mean temperature change curve.
For in scheme in this patent diversiform-leaved poplar seed treatment step and building diversiform-leaved poplar cultivating seeds model step setting with
Lower embodiment:
Embodiment 1
Diversiform-leaved poplar seed is impregnated into 30min in plant nutrition liquid, plant nutrition liquid includes diversiform-leaved poplar nursery stock extracting solution；It is impregnating
Ultrasonication is carried out in the process while also, and the power of ultrasonic wave is 150W, each ultrasound 6s, is divided between adjacent ultrasound twice
20s；
Plant nutrition liquid is conventional commercial nutrient solution；
It constructs diversiform-leaved poplar cultivating seeds model: 20 diversiform-leaved poplar seeds being pressed into circular arrangement in culture medium, are added in culture medium
Soil conditioner, component includes: the soil conditioner raw material in parts by weight
100 parts of diatomite, 80 parts of flyash, 70 parts of bentonite, 50 parts of kaolin, 80 parts of turf, plant straw powder 120
It is part, the part of carrier 12 containing algae, 40 parts of organic fertilizer, 40 parts of humus, 20 parts of zeolite powder, 20 parts of glass microballoon, 18 parts of Cab-O-sil, hydrophobic
Property 3 parts of resin, 20 parts of microbial inoculum；
10 parts of POLYPROPYLENE GLYCOL, 10 parts of urea, 8 parts of monoammonium phosphate, 30 parts of waste residue of Chinese herbs powder, useless 20 parts of tea grounds, fulvic acid are dense
8 parts of contracting liquid, 40 parts of vinasse, 30 parts of leaf mould, 40 parts of sawdust, 20 parts of rice chaff ash, loose 10 parts of phosphorus, 50 parts of coco bran and slow-release fertilizer 30
Part；
Wherein, plant straw powder includes following component by weight percentage:
Milled powders of cornstalk 8%, maize cob meal 14%, potato straw powder 25%, sea-buckthorn straw powder 10%, camel thorn straw powder
10%, rice straw powder 10%, flax straw powder 8%, wheat stalk powder 5%, broomcorn straw powder 5% and pea straw powder 5%；
Microbial inoculum is by actinomyces, nitrogen-fixing bacteria, bacillus subtilis, photosynthetic bacteria, saccharomycete, huge gemma bar
Bacterium solution after bacterium, sulfate reducing bacteria cultivation and fermentation；
Carrier containing algae, which is negative, to be loaded with total ball algae, false ball algae altogether, column silk algae, Scytonema arcangelii, sheath silk algae, micro- sheath algae, anabena, glues
The macromolecule polymeric material of ball algae and nostoc.
Preparation method
S31, kaolin and flyash are uniformly mixed, and it is agglomerating to be added water stirring, under the conditions of dough is placed in 32 DEG C plus
Heat 1.5 hours, obtains the first mixture；
S32, fulvic acid concentrate is added after mixing diatomite and turf, adding water to continue to mix (can extremely flow
It is thick), obtain the second mixture；
S33, plant straw powder is added to the decomposed rear and organic fertilizer of straw decomposing inoculant, humus, waste residue of Chinese herbs powder and is given up
Tea grounds mixing, and mix after coco bran mixes and mix thoroughly with vinasse, leaf mould, sawdust, rice chaff ash and loose phosphorus after heap fermentation, and in 25
DEG C stand 3 days, obtain third mixture；
S34, will zeolite powder, glass microballoon and Cab-O-sil mix after be added hydrophobic resin stir evenly；It is mixed to obtain the 4th
Close material；
S35, urea, monoammonium phosphate and slow-release fertilizer is added after mixing second mixture, the third mixture, and
Dispersion stirring is carried out, then first mixture and the 4th mixture are added thereto, is stirring evenly and then adding into and is carried containing algae
Body and microbial inoculum continue to pave to 28 DEG C of environment lower 4 hours after mixing, and obtain pre- powder, i.e. soil conditioner；
The data of other step matrix salt contents obtain and sprout capacity data acquisition, sprouting week according to diversiform-leaved poplar seed
Growth rate and matrix salt content building both sides relation curve in phase is identical as previous embodiment.
It finally found that there are 17 normal seed germinations in totally 20 diversiform-leaved poplar seeds, after rejecting abnormal seed sprouting data
It obtains diversiform-leaved poplar seed and is sprouting growth rate and matrix salt content building both sides relation curve in the period.
Embodiment 2
Diversiform-leaved poplar seed is impregnated into 90min in plant nutrition liquid, plant nutrition liquid includes diversiform-leaved poplar nursery stock extracting solution；It is impregnating
Ultrasonication is carried out in the process while also, and the power of ultrasonic wave is 200W, each ultrasound 8s, is divided between adjacent ultrasound twice
30s；
Plant nutrition liquid is conventional commercial nutrient solution
It constructs diversiform-leaved poplar cultivating seeds model: 40 diversiform-leaved poplar seeds being pressed into circular arrangement in culture medium, are added in culture medium
Soil conditioner, component includes: the soil conditioner raw material in parts by weight
Soil conditioner is made for following raw materials: 120 parts of diatomite, 100 parts of flyash, 80 parts of bentonite, kaolin 60
Part, 100 parts of turf, 140 parts of plant straw powder, 16 parts of carrier containing algae, 50 parts of organic fertilizer, 13 parts of coating grass-seed, humus 55
Part, 30 parts of zeolite powder, 25 parts of glass microballoon, 20 parts of Cab-O-sil, 4 parts of hydrophobic resin, 25 parts of microbial inoculum；
15 parts of POLYPROPYLENE GLYCOL, 12 parts of urea, 12 parts of monoammonium phosphate, 35 parts of waste residue of Chinese herbs powder, useless 30 parts of tea grounds, fulvic acid are dense
16 parts of contracting liquid, 50 parts of vinasse, 45 parts of leaf mould, 50 parts of sawdust, 250 parts of rice chaff ash, loose 20 parts of phosphorus, 60 parts of coco bran and slow-release fertilizer 35
Part.
Wherein, plant straw powder includes following component by weight percentage:
Milled powders of cornstalk 8%, maize cob meal 14%, potato straw powder 25%, sea-buckthorn straw powder 10%, camel thorn straw powder
10%, rice straw powder 10%, flax straw powder 8%, wheat stalk powder 5%, broomcorn straw powder 5% and pea straw powder 5%；
Microbial inoculum is by actinomyces, nitrogen-fixing bacteria, bacillus subtilis, photosynthetic bacteria, saccharomycete, huge gemma bar
Bacterium solution after bacterium, sulfate reducing bacteria cultivation and fermentation；
Carrier containing algae, which is negative, to be loaded with total ball algae, false ball algae altogether, column silk algae, Scytonema arcangelii, sheath silk algae, micro- sheath algae, anabena, glues
The macromolecule polymeric material of ball algae and nostoc.
The data of other step matrix salt contents obtain and sprout capacity data acquisition, sprouting week according to diversiform-leaved poplar seed
Growth rate and matrix salt content building both sides relation curve in phase is identical as previous embodiment.
It finally found that there are 35 normal seed germinations in totally 40 diversiform-leaved poplar seeds, after rejecting abnormal seed sprouting data
It obtains diversiform-leaved poplar seed and is sprouting growth rate and matrix salt content building both sides relation curve in the period.
Comparative example 1
Added diversiform-leaved poplar nursery stock extracting solution is prepared without everfermentation in this comparative example, other same embodiments of process
2。
It finally found that there are 20 normal seed germinations in totally 40 diversiform-leaved poplar seeds, after rejecting abnormal seed sprouting data
It obtains diversiform-leaved poplar seed and is sprouting growth rate and matrix salt content building both sides relation curve in the period.
Comparative example 2
Diversiform-leaved poplar seed is impregnated without plant nutrition liquid in this comparative example, does not also carry out ultrasonication, other processes
With embodiment 1.
It finally found that there are 7 normal seed germinations in totally 20 diversiform-leaved poplar seeds, reject after abnormal seed sprouts data and obtain
Growth rate and matrix salt content building both sides relation curve in the period are being sprouted to diversiform-leaved poplar seed.
Comparative example 3
Soil conditioner is not added in this comparative example in culture medium, other processes are the same as embodiment 2.
It finally found that there are 23 normal seed germinations in totally 40 diversiform-leaved poplar seeds, after rejecting abnormal seed sprouting data
It obtains diversiform-leaved poplar seed and is sprouting growth rate and matrix salt content building both sides relation curve in the period.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention
Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of method that test diversiform-leaved poplar seed sprouts ability and matrix salt content relationship, which is characterized in that including following step
It is rapid:
Diversiform-leaved poplar seed treatment: diversiform-leaved poplar seed is impregnated into 10min-120min in plant nutrition liquid, the plant nutrition liquid includes
Diversiform-leaved poplar nursery stock extracting solution；Also carry out ultrasonication simultaneously in soaking process, the power of ultrasonic wave is 100-200W, super every time
Sound 5-8s is divided into 20-30s between adjacent ultrasound twice；
It constructs diversiform-leaved poplar cultivating seeds model: diversiform-leaved poplar seed being pressed into circular arrangement in culture medium, soil improvement is added in culture medium
Agent, component includes: the soil conditioner raw material in parts by weight
100-150 parts of diatomite, 80-120 parts of flyash, 70-90 parts of bentonite, 50-70 parts of kaolin, 80-120 parts of turf,
120-160 parts of plant straw powder, -20 parts of carrier 12 containing algae, 40-60 parts of organic fertilizer, 40-70 parts of humus, zeolite powder 20-35
Part, 20-30 parts of glass microballoon, 18-25 parts of Cab-O-sil, 3-5 parts of hydrophobic resin, 20-30 parts of microbial inoculum；
10-20 parts of POLYPROPYLENE GLYCOL, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of waste residue of Chinese herbs powder, useless tea grounds 20-40
Part, 8-24 parts of fulvic acid concentrate, 40-60 parts of vinasse, 30-60 parts of leaf mould, 40-60 parts of sawdust, 20-30 parts of rice chaff ash, pine
10-30 parts of phosphorus, 50-70 parts of coco bran and 30-40 parts of slow-release fertilizer；
It sprouts capacity data to obtain: being sprouted in the period in diversiform-leaved poplar seed, diversiform-leaved poplar cultivating seeds to be measured are obtained by spatial digitizer
The time three-dimensional model diagram of model；The time three-dimensional model diagram includes several diversiform-leaved poplar cultivating seeds models to be measured in different moments
Corresponding three-dimensional model diagram；It is analyzed and is handled by time three-dimensional model diagram, and then the diversiform-leaved poplar kind is calculated
Son cultivates diversiform-leaved poplar seed in model and is sprouting the growth rate in the period；
The data acquisition of matrix salt content: it is sprouted in the period in diversiform-leaved poplar seed, acquires the diversiform-leaved poplar cultivating seeds model to be measured
Culture substrate sample around middle diversiform-leaved poplar seed；Salt in the culture substrate sample around diversiform-leaved poplar seed is measured by salinity tester
Divide content data, matrix salinity then is calculated by salt content data in the culture substrate sample around diversiform-leaved poplar seed and contains
Measure data；
Growth rate and matrix salt content building both sides relation curve in the period are being sprouted according to diversiform-leaved poplar seed.
2. the method that test diversiform-leaved poplar seed according to claim 1 sprouts ability and matrix salt content relationship, feature
Be, in the diversiform-leaved poplar cultivating seeds model, adjacent diversiform-leaved poplar seed spacing be no more than 15cm, and in culture medium diversiform-leaved poplar seed number
Amount is no more than 40；
In sprouting capacity data acquisition process, rejects diversiform-leaved poplar seed and sprout ability abnormal data.
3. the method that test diversiform-leaved poplar seed according to claim 2 sprouts ability and matrix salt content relationship, feature
It is, is analyzed and handled by time three-dimensional model diagram, and then diversiform-leaved poplar in the diversiform-leaved poplar cultivating seeds model is calculated
Seed is sprouting the growth rate in the period, comprising:
The three-dimensional coordinate of acquisition time three-dimensional model diagram obtains apex coordinate in tri- axis direction of XYZ；
According to apex coordinate, diversiform-leaved poplar seed is calculated and is sprouting the growth rate in the period:
。
4. the method that test diversiform-leaved poplar seed according to claim 2 sprouts ability and matrix salt content relationship, feature
It is, is analyzed and handled by time three-dimensional model diagram, and then diversiform-leaved poplar in the diversiform-leaved poplar cultivating seeds model is calculated
Seed is sprouting the growth rate in the period, comprising:
The three-dimensional coordinate of acquisition time three-dimensional model diagram, and then obtain three-dimensional model diagram projected area in tri- axis directions of XYZ；
According to projected area in tri- axis directions of XYZ, diversiform-leaved poplar seed is calculated and is sprouting the growth rate in the period:
。
5. the method for ability and matrix salt content relationship is sprouted according to any test diversiform-leaved poplar seed of claim 3 to 4,
It is characterized in that, the acquisition position of the culture substrate sample be the upper end of diversiform-leaved poplar seed centre coordinate, lower end, left end, right end,
Front end, rear end.
6. the method that test diversiform-leaved poplar seed according to claim 5 sprouts ability and matrix salt content relationship, feature
It is, the matrix salt content data are calculated by following equation:
Ct=KOn*Wt On+KUnder*Wt Under+KIt is left*Wt It is left+KIt is right*Wt It is right+KBefore*Wt Before+KAfterwards*Wt Afterwards。
7. the method that test diversiform-leaved poplar seed according to claim 6 sprouts ability and matrix salt content relationship, feature
Be, according to diversiform-leaved poplar seed sprout the period in growth rate and matrix salt content data building both sides relation curve it
Afterwards, salinity calibration model is constructed:
It is sprouted in the period in diversiform-leaved poplar seed, acquires the upper end of diversiform-leaved poplar seed neighboring seeds centre coordinate, lower end, left end, right end, preceding
The culture substrate sample at end, rear end measures salt content data in the culture substrate sample of neighboring seeds by salinity tester,
Then salinity correction coefficient is calculated；
According to salinity correction coefficient to diversiform-leaved poplar seed sprout the period in growth rate and matrix salt content relation curve into
Row amendment.
8. the method that test diversiform-leaved poplar seed according to claim 7 sprouts ability and matrix salt content relationship, feature
It is, in the calculation formula of the matrix salt content data:
。
9. the method that test diversiform-leaved poplar seed according to claim 8 sprouts ability and matrix salt content relationship, feature
It is, the measurement for sprouting capacity data is carried out on the model established in 3-matic Top-Down Design software.
10. the method that test diversiform-leaved poplar seed according to claim 9 sprouts ability and matrix salt content relationship, feature
Be, according to diversiform-leaved poplar seed sprout the period in growth rate and matrix salt content data building both sides relation curve it
Building temperature correction model and moisture calibration model afterwards, the temperature correction system obtained by temperature correction model and moisture calibration model
Several and moisture correction coefficient；
Growth rate and matrix salinity in the period are being sprouted to diversiform-leaved poplar seed according to temperature correction facotor and moisture correction coefficient
Relation with contents curve is modified.
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