A kind of method improving Hua Shallot seed seedling-raising survival rate
Technical field
What the present invention relates to is a kind of method improving Hua Shallot seed seedling-raising survival rate.
Background technology
Hua Shallot, popular name: electric light flower, lamp sound flower (illiteracy name).Perennial herb, root is crawled, cylindric, multifilament shape fibrous root.Stem is upright, and high 0.5-1 rice, without hair or by thin pubescence.For medicinal plant, there is following effect: Total saponin contained by antiatherogenic effect, remarkable inhibitory action is had to rabbit experiment cholesterol atherosclerotic, show as cholesterol content in blood significantly to reduce, lecithin/cholesterol coefficient increases, skin, cornea, sclera, artery, liver and other internal organ lipoids calmness reduces, intravenous injection and oral all effective, not only there is prevention effect, and having therapeutic action, pathological anatomy visible vessels, the heart, liver, nephropathy all comparatively control group are light.To the effect of cardiovascular and respiratory system to blood pressure that is normal and cholesterol artery younger brother sample sclerosis rabbit, Jacob's ladder saponin, all without obvious effect, is breathed slightly deeply, the increase of heart shrinkage amplitude, frequency deceleration.
At present, the seedling growing process about Hua Shallot is mainly sexual propagation, and for the technology that sexual propagation and seed seedling-raising are bred, prior art also has report, but the single survival rate of technology of prior art Dui Hua Shallot seed seedling-raising is low.
Summary of the invention
The present invention is directed to existing deficiency and provide a kind of method improving Hua Shallot seed seedling-raising survival rate.
The present invention realizes above-mentioned purpose by the following method.
Improve a method for Hua Shallot seed seedling-raising survival rate, the method comprises the following steps:
(1) gather seed: after fruit harvesting, washing with water several all over filtering, removing flat kind of foreign material, obtaining pure seed, seed is spread out, at room temperature dry in the shade;
(2) plant leaching liquor is prepared: choose well-developed climbing groundsel plant, gather its root, stem, leaf, shred with scissors after drying in the shade, take respectively root, stem, leaf 7.5,3.5,5g, add 100 times of distilled water, 25 ~ 30 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, obtain concentrate with Rotary Evaporators reduction vaporization again, be settled to 100ml; 50ppm gibberellin is added and 35ppm salicylic acid obtains leaching liquor in concentrate after constant volume 100ml;
Pretreatment before sowing: before sowing, seed is soaked 2h in described leaching liquor, then put into 50% tpn wetting powder, 500 times of liquid after the cleaning of seed warm water to soak 0.5 hour, after seed tiled one deck in culture plate, carry out illumination cultivation, intensity of illumination 1200-1400LX, light application time is 8 hours/day, and temperature is 22-24 DEG C, in incubation, culture plate keeps moistening, transplants after seed sprouting.
Beneficial effect:
The present invention fully utilizes growth regulator, salicylic acid and Appropriate application illumination etc. and breaks seed dormant technology, make to utilize the present invention to obtain the seedling from seed well developed root system of Hua Shallot, and growing way is good, speed is fast, survival rate is high, particularly after containing climbing groundsel leaching liquor and growth regulator, salicylic immersion, greatly improve the sprouting survival rate of Hua Shallot seed.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, and following each embodiment, only for illustration of the present invention, does not limit the present invention.
Embodiment one
Improve a method for Hua Shallot seed seedling-raising survival rate, the method comprises the following steps:
(1) gather seed: after fruit harvesting, washing with water several all over filtering, removing flat kind of foreign material, obtaining pure seed, seed is spread out, at room temperature dry in the shade;
(2) plant leaching liquor is prepared: choose well-developed climbing groundsel plant, gather its root, stem, leaf, shred with scissors after drying in the shade, take respectively root, stem, leaf 7.5,3.5,5g, add 100 times of distilled water, 25 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, obtain concentrate with Rotary Evaporators reduction vaporization again, be settled to 100ml; 50ppm gibberellin is added and 35ppm salicylic acid obtains leaching liquor in concentrate after constant volume 100ml;
Pretreatment before sowing: before sowing, seed is soaked 2h in described leaching liquor, then put into 50% tpn wetting powder, 500 times of liquid after the cleaning of seed warm water to soak 0.5 hour, after seed tiled one deck in culture plate, carry out illumination cultivation, intensity of illumination 1200LX, light application time is 8 hours/day, and temperature is 22 DEG C, in incubation, culture plate keeps moistening, transplants after seed sprouting.
Embodiment two
Improve a method for Hua Shallot seed seedling-raising survival rate, the method comprises the following steps:
(1) gather seed: after fruit harvesting, washing with water several all over filtering, removing flat kind of foreign material, obtaining pure seed, seed is spread out, at room temperature dry in the shade;
(2) plant leaching liquor is prepared: choose well-developed climbing groundsel plant, gather its root, stem, leaf, shred with scissors after drying in the shade, take respectively root, stem, leaf 7.5,3.5,5g, add 100 times of distilled water, 30 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, obtain concentrate with Rotary Evaporators reduction vaporization again, be settled to 100ml; 50ppm gibberellin is added and 35ppm salicylic acid obtains leaching liquor in concentrate after constant volume 100ml;
Pretreatment before sowing: before sowing, seed is soaked 2h in described leaching liquor, then put into 50% tpn wetting powder, 500 times of liquid after the cleaning of seed warm water to soak 0.5 hour, after seed tiled one deck in culture plate, carry out illumination cultivation, intensity of illumination 1400LX, light application time is 8 hours/day, and temperature is 24 DEG C, in incubation, culture plate keeps moistening, transplants after seed sprouting.
Wherein, adopt 100 Li Hua Shallot seeds to test in process of the test respectively, contrast adopts conventional seeding growing seedlings method, and result shows, and the seed germination rate of embodiment 1,2 reaches 94% and 93% respectively, and to contrast germination rate be 70%.