CN104430517A - Salt leaching resistance seeding agent of basil and preparation method of salt leaching resistance seeding agent - Google Patents
Salt leaching resistance seeding agent of basil and preparation method of salt leaching resistance seeding agent Download PDFInfo
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- CN104430517A CN104430517A CN201410687253.0A CN201410687253A CN104430517A CN 104430517 A CN104430517 A CN 104430517A CN 201410687253 A CN201410687253 A CN 201410687253A CN 104430517 A CN104430517 A CN 104430517A
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Abstract
The invention discloses a salt leaching resistance seeding agent of basil and a preparation method of the salt leaching resistance seeding agent. The salt leaching resistance seeding agent comprises the following ingredients at mass concentrations in a solution : 0.2-1.5g/L vitamin C, 3-15g/L calcium nitrate, 0.5-5g/L sucrose, 5-22g/L potassium iodate, 0.02-0.5g/L brassinolide, 5-10ml/L alcohol and the balance of water. The salt leaching resistance seeding agent of the basil has a reasonable matching ratio and no toxic or side effects and is safe, and can effectively promote basil seed germination under salt stress, and increase the germination percentage of seeds.
Description
Technical field
The present invention relates to field of plant growing technology, be specifically related to anti-salt seed soaking agent of a kind of sweet basil and preparation method thereof.
Background technology
Salinity is one of abiotic stress factor of limiting plant growth and output increased.According to statistics, about have in the world the anthropogenic soil of 23% in various degree be subject to saliferous harm, China's current saline land area is about 9,913 ten thousand hm
2, and present increase situation due to the unreasonable development soil salinization.Wang Yuping etc. to sprout cauliflower seed under salt stress and affecting of Physiological Characteristics one the article pointed out at salicylic acid: plant has different in the response of Different growth phases to salt stress, and Their Seed Germinating Period period that to be salt resistance ability relatively weak.For the plantation of crop, seed sprouting quality directly affects plant growth and economic benefit.Salt marsh surround inhibition seed moisture content absorbs, and reduces cell permeability, thus produces toxic effect to the many physiology of seed, biochemical metabolism effect.
Sweet basil (Ocimum Basilicum L.), also known as south Sinkiang ziziphora herb, FRUCTUS OCIMI, holy basil etc., for Labiatae annual herb plant, be distributed widely in the tropical and subtropical region of Asia, Africa and Central and South America, all have cultivation on the Guangdong of China, Guangxi, Henan, Sichuan, Fujian and other places.Sweet basil is a kind of important fragrant plant, the function of have dispelling wind promoting the circulation of qi, dampness elimination helps digestion, invigorate blood circulation, detoxifying.For exogenous headache, food flatulence stagnant, gastral cavity pain, have loose bowels, treatment that irregular menstruation, traumatic injury, snake bite and insect sting, skin wet the disease such as sore, urticaria sufferings, have edible and medical value; The Basil Essential of its blade and stem's glandular secretion is widely used as flavor enhancement and the field such as medicine and industry of food service industry.Research shows, salt stress significantly suppresses ocimi seeds to be sprouted.Therefore, alleviate salt stress to the injury effect of sweet basil bud phase, improve percentage of seedgermination, for raising sweet basil output and economic benefit thereof, there is important function.Current existing ocimi seeds seed soaking agent is all single compounds, and only has certain promotion for the sprouting of ocimi seeds under normal condition, and rarely has the seed soaking agent for the multiple compounds combination improving ocimi seeds germination rate under salt marsh environment.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide the anti-salt seed soaking agent of a kind of sweet basil, reasonable mixture ratio, safe without toxic side effect, in order to promote that ocimi seeds is sprouted under salt stress, to improve percentage of seedgermination.
Another object of the present invention is to provide the anti-salt of a kind of sweet basil to urge to sprout the preparation method of seed soaking agent, by controlling the order of addition of component, to obtain a kind of reasonable mixture ratio, the anti-salt of safe without toxic side effect sweet basil short sprouting seed soaking agent.
For solving the problem, the technical solution adopted in the present invention is as follows:
The anti-salt seed soaking agent of a kind of sweet basil, it is made up of vitamin C, nitrate of lime, sucrose, Potassiumiodate, brassinosteroid and second alcohol and water, and wherein each component mass concentration is in the solution respectively:
Vitamin C: 0.2-1.5g/L
Nitrate of lime: 3-15g/L
Sucrose: 0.5-5g/L
Potassiumiodate: 5-22g/L
Brassinosteroid: 0.02-0.5g/L
Ethanol: 5-10ml/L,
Solvent is water.
As preferred scheme, in the anti-salt seed soaking agent of above-mentioned sweet basil, each component mass concentration is in the solution respectively:
Vitamin C: 0.26-1.23g/L
Nitrate of lime: 3.28-13.12g/L
Sucrose: 0.51-3.09g/L
Potassiumiodate: 5.35-21.4g/L
Brassinosteroid: 0.024-0.48g/L
Ethanol: 5-10ml/L,
Solvent is water.
As preferred scheme, in the anti-salt seed soaking agent of above-mentioned sweet basil, each component mass concentration is in the solution respectively: vitamin C 0.88g/L; Nitrate of lime 13.12g/L; Sucrose 0.51g/L; Potassiumiodate 21.4g/L; Brassinosteroid 0.48g/L; Ethanol 10ml/L, solvent is water.
In such scheme, particularly, the ethanol of described alcohol 95 % or absolute ethyl alcohol.
In the present invention, nitrate of lime Ca (NO
3)
2middle Ca
2+effectively can reduce the malonaldehyde in salt-stressed plants body and Electrolytic leakage, suppress Na
+absorption, maintain plant cell film integrality and stability, reduce the toxic effect of environment salt stress.Ca (NO
3)
2middle NO
3 -with Cl
-antagonism is there is, i.e. NO in plant absorption process
3 -ion effectively can reduce Cl
-absorptive amount, and then reduce Cl
-toxic action.Under salt stress, a large amount of Na
+remarkable suppression plant is to K
+absorption, Potassiumiodate (KIO in the present invention
3) K is provided
+, effectively can alleviate Na
+with K
+competitive absorption, improve K/Na ratio in plant corpus, maintain ionic equilibrium, and then improve plant corpus salt resistance; IO
3 -effectively can improve the content of antioxidase and non-enzyme polyphenoils in Plants under Salt Stress body, remove the active oxygen produced in Plants under Salt Stress body, improve plant salt tolerance ability.Vitamin C is antioxidant, and sucrose is the signaling molecule of regulating growth of plants, and brassinosteroid is a kind of chemistry and life.Under salt stress, vitamin C, sucrose or brassinosteroid are combined, activities of antioxidant enzymes in plant corpus can be significantly improved, reduce content of oxygen free radical, Promoting plant growth, increase plant to the resistance of salinity.
A preparation method for the anti-salt seed soaking agent of sweet basil, its step is as follows:
1) vitamin C is taken soluble in water;
2) take that sucrose is soluble in water is dissolved in step 1) solution in;
3) in step 2) solution in add nitrate of lime, Potassiumiodate makes seed soaking agent mother liquor;
4) take brassinosteroid to be dissolved in ethanol, and with step 3) mother liquor mix;
5) finally with water, solution is settled to target volume.
Compared to existing technology, beneficial effect of the present invention is:
1. cultivation produced by the sweet basil that the anti-salt seed soaking agent of sweet basil of the present invention is applied to saline soil area, reduces the harm of salt stress, efficiently solves ocimi seeds under salt marsh environment and to germinate slow, low, the even ablastous problem of germination rate;
2. soak seed before adopting the anti-salt seed soaking agent of sweet basil of the present invention to broadcast seed, accelerate ocimi seeds speed of germination, improve germination rate;
3. sweet basil of the present invention anti-salt seed soaking agent formula rationally, have no side effect, preparation method and use simple to operate, easy.
Below in conjunction with accompanying drawing and embodiment, the present invention is described in further detail.
Accompanying drawing explanation
Fig. 1 is the result of use comparison diagram of the embodiment of the present invention 1;
Fig. 2 is the result of use comparison diagram of the embodiment of the present invention 2;
Fig. 3 is the result of use comparison diagram of the embodiment of the present invention 3.
Embodiment
embodiment 1
The anti-salt seed soaking agent of a kind of sweet basil, it is by vitamin C, 0.264g/L; Ca (NO
3)
2, 8.2g/L; Sucrose, 3.09g/L; KIO
3, 8.56g/L; Brassinosteroid, 0.024g/L; 95% ethanol, 5ml; All the other are water composition.
Its preparation method is: 1. take vitamin C soluble in water; 2. sucrose is taken soluble in water; 3. Ca (NO is added
3)
2, KIO
3make seed soaking agent mother liquor; 4. take brassinosteroid and be dissolved in 95% ethanolic solution, and mix with solution above; 5. solution is settled to target volume by last water.
Using method is as follows: thin up in use, and extension rate is 50 times of the anti-salt seed soaking agent of sweet basil.
Comparative example 1
Under 30% seawater stress, the sweet basil of clore basil, purple sweet basil and large Ye Luole tri-kinds of different cultivars is adopted to the seed soaking agent seed soaking of embodiment 1, and correspondence is often planted sweet basil and is done the blank that does not adopt the seed soaking agent of above-described embodiment 1; Result of the test is see Fig. 1.
The result display of Fig. 1, after adopting above-mentioned seed soaking agent to soak seed, under 30% seawater stress, clore basil, purple sweet basil and large Ye Luole germination rate are respectively 58%, 59% and 50%, compare control group germination rate and improve 41%, 47% and 55% respectively, seed germinating time shifts to an earlier date 2 days.
embodiment 2
The anti-salt seed soaking agent of a kind of sweet basil, it is by vitamin, 1.23g/L; Ca (NO
3)
23.28g/L; Sucrose 2.0g/L; KIO
3, 5.35g/L; Brassinosteroid, 0.24g/L; 95% ethanol, 8ml; All the other are water composition.
Its preparation method is: 1. take vitamin C soluble in water; 2. sucrose is taken soluble in water; 3. Ca (NO is added
3)
2, KIO
3make seed soaking agent mother liquor; 4. take brassinosteroid and be dissolved in ethanol solution, and mix with solution above; 5. solution is settled to target volume by last water.
Its using method is as follows: thin up in use, and extension rate is 80 times of the anti-salt seed soaking agent of sweet basil.
Comparative example 2
Under 30% seawater stress, the sweet basil of clore basil, purple sweet basil and large Ye Luole tri-kinds of different cultivars is adopted to the seed soaking agent seed soaking of embodiment 2, and correspondence is often planted sweet basil and is done the blank that does not adopt the seed soaking agent of above-described embodiment 2; Result of the test is see Fig. 2.
Fig. 2 result shows: after adopting the seed soaking agent seed soaking of embodiment 2, under 30% seawater stress, clore basil, purple sweet basil and large Ye Luole germination rate are respectively 58%, 59% and 50%, and compare control group germination rate and improve 35%, 50% and 60% respectively, seed germinating time shifts to an earlier date 2 days.
embodiment 3
The anti-salt seed soaking agent of a kind of sweet basil, it is by vitamin C, 0.88g/L; Ca (NO
3)
2, 13.12g/L; Sucrose, 0.51g/L; KIO
3, 21.4g/L; Brassinosteroid, 0.48g/L; 95% ethanol, 10ml; All the other are water composition.
Its preparation method is: 1. take vitamin C soluble in water; 2. sucrose is taken soluble in water; 3. Ca (NO is added
3)
2, KIO
3make seed soaking agent mother liquor; 4. take brassinosteroid and be dissolved in 95% ethanolic solution, and mix with solution above; 5. solution is settled to target volume by last water.
Using method is as follows: add running water dilution in use, extension rate is 100 times of the anti-salt seed soaking agent of sweet basil.
Comparative example 3
Under 30% seawater stress, the sweet basil of clore basil, purple sweet basil and large Ye Luole tri-kinds of different cultivars is adopted to the seed soaking agent seed soaking of embodiment 3, and correspondence is often planted sweet basil and is done the blank that does not adopt the seed soaking agent of above-described embodiment 3; Result of the test is see Fig. 3.
Fig. 3 result shows: after adopting the seed soaking agent seed soaking of embodiment 3, under 30% seawater stress, clore basil, purple sweet basil and large Ye Luole germination rate are respectively 58%, 59% and 50%, and compare control group germination rate and improve 45%, 51% and 54% respectively, seed germinating time shifts to an earlier date 3 days.
Above-mentioned embodiment is only the preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, change and the replacement of any unsubstantiality that those skilled in the art does on basis of the present invention all belong to the present invention's scope required for protection.
Claims (5)
1. the anti-salt seed soaking agent of sweet basil, is characterized in that, it is made up of vitamin C, nitrate of lime, sucrose, Potassiumiodate, brassinosteroid and second alcohol and water, and wherein each component mass concentration is in the solution respectively:
Vitamin C: 0.2-1.5g/L
Nitrate of lime: 3-15g/L
Sucrose: 0.5-5g/L
Potassiumiodate: 5-22g/L
Brassinosteroid: 0.02-0.5g/L
Ethanol: 5-10ml/L,
Solvent is water.
2. the anti-salt seed soaking agent of sweet basil according to claim 1, is characterized in that, wherein each component mass concentration is in the solution respectively:
Vitamin C: 0.26-1.23g/L
Nitrate of lime: 3.28-13.12g/L
Sucrose: 0.51-3.09g/L
Potassiumiodate: 5.35-21.4g/L
Brassinosteroid: 0.024-0.48g/L
Ethanol: 5-10ml/L,
Solvent is water.
3. the anti-salt seed soaking agent of sweet basil according to claim 1, is characterized in that, wherein each component mass concentration is in the solution respectively:
Vitamin C 0.88g/L; Nitrate of lime 13.12g/L; Sucrose 0.51g/L; Potassiumiodate 21.4g/L; Brassinosteroid 0.48g/L; Ethanol, 10ml/L, solvent is water.
4. the anti-salt seed soaking agent of sweet basil according to claim 1, is characterized in that, the ethanol of described alcohol 95 % or absolute ethyl alcohol.
5. a preparation method for the anti-salt seed soaking agent of sweet basil as claimed in claim 1, it is characterized in that, its step is as follows:
1) vitamin C is taken soluble in water;
2) take that sucrose is soluble in water is dissolved in step 1) solution in;
3) in step 2) solution in add nitrate of lime, Potassiumiodate makes seed soaking agent mother liquor;
4) take brassinosteroid to be dissolved in ethanol, and with step 3) mother liquor mix;
5) finally with water, solution is settled to target volume.
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|---|---|---|---|
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|---|---|---|---|
| CN201410687253.0A CN104430517B (en) | 2014-11-24 | 2014-11-24 | A kind of sweet basil salt resistance seed soaking agent and preparation method thereof |
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Cited By (3)
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| WO2018123651A1 (en) * | 2016-12-27 | 2018-07-05 | 国立研究開発法人理化学研究所 | Agent for improving environmental stress tolerance of plant |
| CN110074114A (en) * | 2019-04-29 | 2019-08-02 | 天津市汉邦植物保护剂有限责任公司 | A kind of brassinolide-containing, aqua of sugar alcohol calcium and preparation method and application |
| CN115245175A (en) * | 2022-08-30 | 2022-10-28 | 淮阴师范学院 | Seed treating agent and preparation method and application thereof |
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| CN103798285A (en) * | 2014-01-24 | 2014-05-21 | 中国农业科学院草原研究所 | Alfalfa seed soaking liquid and related method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018123651A1 (en) * | 2016-12-27 | 2018-07-05 | 国立研究開発法人理化学研究所 | Agent for improving environmental stress tolerance of plant |
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| CN110074114A (en) * | 2019-04-29 | 2019-08-02 | 天津市汉邦植物保护剂有限责任公司 | A kind of brassinolide-containing, aqua of sugar alcohol calcium and preparation method and application |
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| CN115245175B (en) * | 2022-08-30 | 2023-10-10 | 淮阴师范学院 | Seed treating agent and preparation method and application thereof |
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| CN104430517B (en) | 2017-07-07 |
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