CN102714991A - Lycopodium serratum cutting propagation method - Google Patents
Lycopodium serratum cutting propagation method Download PDFInfo
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- CN102714991A CN102714991A CN2011100785978A CN201110078597A CN102714991A CN 102714991 A CN102714991 A CN 102714991A CN 2011100785978 A CN2011100785978 A CN 2011100785978A CN 201110078597 A CN201110078597 A CN 201110078597A CN 102714991 A CN102714991 A CN 102714991A
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- cuttage
- clubmoss herb
- propagation method
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Abstract
The invention relates to a lycopodium serratum cutting propagation method which comprises the following steps: soaking lycopodium serratum branch roots with brassinolide with the concentration of 0.8-1.5mg/kg for for 0.5h-1h; and selecting 0-10cm surface soil of an original habitat, adding vermiculite according to the proportion of 1:1, and fully mixing to obtain cutting matrix. The lycopodium serratum cutting propagation method has the advantages that an agent can be directly dissolved in water without using an organic solvent for dissolving, and is simple and convenient in operation; the dosage is small, and only 0.8-1.5mg brassinolide is needed for 1kg solution, so that the cost is low; the survival rate is as high as above 98%; and roots grow early and at only 42nd days after cutting.
Description
Technical field
The present invention relates to a kind of cultivation of plants propagation method, especially a kind of serrate clubmoss herb cuttage breeding method.
Background technology
Serrate clubmoss herb [Huperzia serrata (Thunb.ex Murray) Trev.] has another name called Huperzia serrata, is Huperziaceae stone araucaria pteridophyte, provinces and regions such as main cloth northeast, the Yangtze river basin and Guizhou Province, Yunnan, osmanthus, Guangdong, Fujian.All herbal medicine, the diseases such as poison, schizophrenia of loosing are used to bring down a fever, stop blooding, subside a swelling.(Huperzine A HupA) has lax striate effect to its active component alkali huperzine on animal experiment, be acetylcholinesteraseinhibitors inhibitors, has good curative effect for treatment myasthenia gravis, person in middle and old age's dementia and raising memory.Product gains great popularity.
Serrate clubmoss herb is special because of growing environment is required, growth is slow and the natural propagation rate is low, and wild resource has been in imminent danger, artificial planting or foster imperatively, and sapling multiplication is to suppress the bottleneck of fostering or planting.According to reported in literature, in the history of life of serrate clubmoss herb, gametophyte needs to obtain nutrition through the fungi effect, and its amphigamy be unable to do without water.Receive such environmental effects big, survival rate is low.Equal and the mycosymbiosis of Huperzia serrata sporophyte and gametophyte, forming mycorhiza could grow, since Huperzia serrata and mycosymbiosis, sterilization difficulty during tissue culture, the requirement condition height, the sterilization success rate is low.Cuttage becomes the good approach of Huperzia serrata artificial propagation.
Brassin lactones (Brassinolide; Abbreviation BR) has another name called brassinosteroid; Separated from rape (Brassica napus) pollen in 1979; Be to have highly active sterol lactone crystal, from the Different Organs of many different plants, separate obtaining BR or its analog later on again successively, be acknowledged as one type of novel plant hormone.In recent years discover that BR can promote cell elongation and cell division, improve photosynthetic ability, promote the differentiation of conduit, the resistance of enhancement of plant influences pollen development and fertility, and improving the yield and quality influences aging course.In tissue culture, in the MS medium, add 10
-5～10
-1The BR of mg/kg can promote the rape hypocotyls differentiation of calli; The young stem callus of tangerine and peach leaf orange is converted into embryo can does sth. in advance the BR of 7-10 days 0.01mg/kg and can make land cotton Corker210,312 differentiation produce embryo callus, and keep vitality and embryo's generating ability of this kind callus effectively.Influence shows that adding the BR callus induction rate among the MS obviously improves to He Yujiong research BR to loosestrife blade callus induction; Than the high 1-6 that does not add hormone doubly; As add 6-BA again; BR can not only obviously improve the generation of cooperative association, and the protein component in the cooperative association is obviously changed, and this each synergistic effect is better than IAA and 6-BA and synergy.Research such as Lu BR is in the experiment that promotes the growth of fox rice grass cooperative association; With MS is minimal medium; Be aided with IAA and 6-BA with BR; At 0.2mg/kg, the 6-B concentration fixed is under 3.0mg/kg the concentration fixed of IAA in discovery, and (BR of 0.03mg/L handles and is fit to fox rice grass callus growth and regeneration higher concentration.Research BR such as Cao Yunying show the result that influences of volume callus growth and differentiation; The BR that adds variable concentrations in the MS+6-BA1.0mg/L+NAA0.1mg/kg medium is (after 0.001～1.0mg/L); Callus fresh weight increasing rate, callus differentiation rate are significantly increased, and concentration is that 0.01mg/L processing callus fresh weight increasing rate is the highest.It is 249% of contrast; It is the highest that concentration 0.1mg/L handles the callus differentiation rate, is 441% of contrast.Can make the green seedling differentiation rate of increase wheat be increased to 47.2% at the BR that induces additional 0.02mg/k on the training base by 25.7% (contrast); Adding 0.1 μ M promotes the grow thickly stem of bud of pimento to prolong; The BR of low concentration 0.005mg/kg handles the growth that is fit to fox rice grass regrowth.BR has inhibitory action to the growth of root of the crop, and its mechanism is that BR can promote ethene to synthesize and then suppress the elongation of root.But the BR of low concentration also can promote the elongation of root and the formation of adventive root sometimes, evidence suggests that endogenous BR has controlled the startup of lateral root.Utilizing BR on the serrate clubmoss herb cottage propagation, to use is not reported.
Summary of the invention
The objective of the invention is to explore a kind of easy to use, practical, technology and method of improving the serrate clubmoss herb cuttage survival rate.
The present invention uses brassin lactones and adopts slow leaching method, control working concentration, soak time, cuttage period, cutting medium, inserts aspect such as fringe, forms the cuttage new method that the serrate clubmoss herb cuttage survival rate is high, easy and simple to handle, consumption is little.
The technical scheme of being taked is:
Using concentration is brassin lactones immersion serrate clubmoss herb branch base portion 0.5h～1h of 0.8～1.5mg/kg; Select the topsoil of former habitat 0～10cm, add vermiculite, make cutting medium after fully mixing in 1: 1 ratio.
Know-why of the present invention:
After serrate clubmoss herb plant cell absorbs certain density brassin lactones, activate the catalyzing enzyme in the body, promoted the sprouting and the growth of root system, under the matrix acting in conjunction that contains serrate clubmoss herb symbiosis endophyte, accelerated the formation of new plant.
Characteristics of the present invention are: (1) this law adopts the long period (0.5～1h) casual method of soaking; (2) low (0.8～1.5mg/kg), cost is low, and is safe for working concentration; (3) IBA dips in the base portion cuttage and will use 95% dissolve with ethanol to dilute, and solvent will specially be equipped with, and operation divides the 2-3 step to carry out; The direct water dissolved dilution of the present invention, operation one step completion, easy to use.(4) use this law serrate clubmoss herb cuttage survival rate to reach more than 98%; (5) this law utilization is made cutting medium after containing former habitat table soil (topsoil of place 0～10cm that wild serrate clubmoss herb distributes, the down with) improvement of serrate clubmoss herb fungal component, has optimized the cuttage environment; (6) using after this law cuttage 42 days promptly begins to take root.
Advantage of the present invention:
Medicament is directly water-soluble, need not use organic solvent (ethanol) dissolving, and is easy and simple to handle; Consumption is little, and every kg solution only needs the brassin lactones of 0.8～1.5mg, and cost is low; Survival rate is up to more than 98%; Go out root early, 42 days begin to take root after the cuttage.
Description of drawings
Fig. 1 is the branch of the using the present invention's technology different length situation map of taking root.
Fig. 2 is the cuttage seeding situation map of taking root.
Embodiment
Practical implementation method of the present invention:
Cuttage ground point selection should be carried out April in spring～May or September in autumn～October in the sylvan life of height above sea level 600m～1200m serrate clubmoss herb normal region, makes cutting medium with native 50% the vermiculite mixture of adding of former habitat table; Selection has terminal bud, is about the slotting fringe of branch work of 3cm; Clip is cut into wedge shape, cuts off the blade of bottom 2/3rds, and using concentration is brassinolide solution immersion branch base portion 0.5h～1h of 0.8～1.5mg/kg; Pick up the cuttage when nothing is dripped of drying in the air; Cut seedling the same day, handled the same day, cuttage on the same day.Note after the cuttage preserving moisture.
Embodiment 1
Sylvan life in height above sea level 600m serrate clubmoss herb normal region was carried out April in spring～May, added 50% vermiculite mixture with former habitat table soil and made cutting medium; Selection has terminal bud, is about the slotting fringe of branch work of 3cm, and clip is cut into wedge shape, cuts off the blade of bottom 2/3rds; Use concentration to soak branch base portion 1h, pick up the cuttage when nothing is dripped of drying in the air, cut seedling the same day as the brassinolide solution of 0.8mg/kg; Handled the same day, cuttage on the same day.Note after the cuttage preserving moisture.42 days begin to take root, and root is sturdy, and survival rate 98% after 65 days.
Embodiment 2
Sylvan life in height above sea level 1200m serrate clubmoss herb normal region was carried out April in spring～May, added 50% vermiculite mixture with former habitat table soil and made cutting medium; Selection has terminal bud, is about the slotting fringe of branch work of 3cm, and clip is cut into wedge shape, cuts off the blade of bottom 2/3rds; Use concentration to soak branch base portion 0.5h, pick up the cuttage when nothing is dripped of drying in the air, cut seedling the same day as the brassinolide solution of 1.5mg/kg; Handled the same day, cuttage on the same day.Note after the cuttage preserving moisture.42 days begin to take root, and root is sturdy, and survival rate 98% after 65 days.
Embodiment 3
Sylvan life in height above sea level 800m serrate clubmoss herb normal region was carried out September in autumn～October, added 50% vermiculite mixture with former habitat table soil and made cutting medium; Selection has terminal bud, is about the slotting fringe of branch work of 3cm, and clip is cut into wedge shape, cuts off the blade of bottom 2/3rds; Use concentration to soak branch base portion 1h, pick up the cuttage when nothing is dripped of drying in the air, cut seedling the same day as the brassinolide solution of 1.0mg/kg; Handled the same day, cuttage on the same day.Note after the cuttage preserving moisture.40 days begin to take root, and root is sturdy, and survival rate 100% after 65 days.
2008, medicinal garden, Guangxi serrate clubmoss herb seminar used this technology, the Da Rong mountain carries out the open country cuttage in Yulin City of Guangxi Zhuang Autonomous in Beiliu City, several 500 strains of cuttage branch, and survival rate is more than 98%.
2009, medicinal garden, Guangxi serrate clubmoss herb seminar used this technology and uses potted plant 30 basins, 600 strain branches on Da Rong mountain, Yulin City of Guangxi Zhuang Autonomous Beliu County, and survival rate reaches 100%; Open country cuttage 2000 strain survival rates reach 99.3%; The same period, Daming Shan Mountain carries out open country cuttage 500 strains in the Nanning city, and survival rate is 99%.
Claims (1)
1. serrate clubmoss herb cuttage breeding method is characterized in that: using concentration is that the brassin lactones of 0.8～1.5mg/kg soaks serrate clubmoss herb branch base portion 0.5h～1h; Select the topsoil of former habitat 0～10cm, add vermiculite, make cutting medium after fully mixing in 1: 1 ratio.
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| CN2011100785978A CN102714991A (en) | 2011-03-30 | 2011-03-30 | Lycopodium serratum cutting propagation method |
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| CN2011100785978A CN102714991A (en) | 2011-03-30 | 2011-03-30 | Lycopodium serratum cutting propagation method |
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| CN102714991A true CN102714991A (en) | 2012-10-10 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106106020A (en) * | 2016-08-31 | 2016-11-16 | 界首市金玖江农业发展有限公司 | A kind of typical continental desert climate region Fructus Vitis viniferae cuttage and seedling culture method |
| CN107548755A (en) * | 2017-10-09 | 2018-01-09 | 吉首大学 | A kind of primary border cuttage breeding method of Huperzia serrata |
| CN108925427A (en) * | 2018-05-31 | 2018-12-04 | 合肥本盛生物科技有限公司 | A method of improving serrate clubmoss herb tufted seedling breeding coefficient |
| CN114027041A (en) * | 2021-11-26 | 2022-02-11 | 浙江理工大学 | Efficient cuttage breeding method for huperzia serrata under natural condition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019487A (en) * | 2006-12-21 | 2007-08-22 | 浙江省中药研究所有限公司 | Imitating wild planting process of Chinese huperzine serrate |
| CN101658124A (en) * | 2009-09-01 | 2010-03-03 | 王忠华 | Rapid propagation method of huperzia serrata |
-
2011
- 2011-03-30 CN CN2011100785978A patent/CN102714991A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019487A (en) * | 2006-12-21 | 2007-08-22 | 浙江省中药研究所有限公司 | Imitating wild planting process of Chinese huperzine serrate |
| CN101658124A (en) * | 2009-09-01 | 2010-03-03 | 王忠华 | Rapid propagation method of huperzia serrata |
Non-Patent Citations (1)
| Title |
|---|
| 冯世鑫 等: "芸苔素内酯对蛇足石杉插穗生根成活的影响", 《中国种业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106106020A (en) * | 2016-08-31 | 2016-11-16 | 界首市金玖江农业发展有限公司 | A kind of typical continental desert climate region Fructus Vitis viniferae cuttage and seedling culture method |
| CN107548755A (en) * | 2017-10-09 | 2018-01-09 | 吉首大学 | A kind of primary border cuttage breeding method of Huperzia serrata |
| CN108925427A (en) * | 2018-05-31 | 2018-12-04 | 合肥本盛生物科技有限公司 | A method of improving serrate clubmoss herb tufted seedling breeding coefficient |
| CN114027041A (en) * | 2021-11-26 | 2022-02-11 | 浙江理工大学 | Efficient cuttage breeding method for huperzia serrata under natural condition |
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Application publication date: 20121010 |