CN108925427A - A method of improving serrate clubmoss herb tufted seedling breeding coefficient - Google Patents
A method of improving serrate clubmoss herb tufted seedling breeding coefficient Download PDFInfo
- Publication number
- CN108925427A CN108925427A CN201810552264.6A CN201810552264A CN108925427A CN 108925427 A CN108925427 A CN 108925427A CN 201810552264 A CN201810552264 A CN 201810552264A CN 108925427 A CN108925427 A CN 108925427A
- Authority
- CN
- China
- Prior art keywords
- explant
- tufted seedling
- culture
- tufted
- clubmoss herb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of methods for improving serrate clubmoss herb tufted seedling breeding coefficient.This method includes wild serrate clubmoss herb sporinite surface sterilizing, prepares the operating procedures such as explant, the culture of explant adaptability, the sterilizing of explant endophyte, the culture of tufted seedling proliferative induction, tufted seedling grown cultures.Aseptic explant tufted seedling proliferative induction success rate of the present invention is 90% or more, and the tufted seedling breeding coefficient that is averaged can achieve 60 or more, and the sterile tufted seedling for cultivating acquisition is used directly for preparation aseptic explant, carries out the circulation-induced proliferation of tufted seedling.The present invention not only increases the proliferative induction success rate and tufted seedling breeding coefficient of serrate clubmoss herb tufted seedling; and a sterilization treatment is achieved with a large amount of aseptic explants; without repeating disinfecting action; reduce microbiological contamination risk; improve working efficiency; simplification of flowsheet, the large-scale production suitable for serrate clubmoss herb.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of to improve serrate clubmoss herb tufted seedling breeding coefficient
Method.
Technical background
Serrate clubmoss herb, scientific name Huperzia serrata are a kind of perennial pteridophytes of Huperziaceae stone araucaria, under natural conditions serrate clubmoss herb
It can be bred by sporogenesis, brood cell's breeding and three kinds of modes of root division propagation, huperzine is the main work of serrate clubmoss herb
One of property ingredient.Huperzine is a kind of reversible acetylcholinesterase inhibitor, can improve the content of acetylcholine in brain,
There is good therapeutic effect to senile dementia, it is whole although the artificial synthesized of huperzine has been achieved with some achievements
A synthesis process complex steps, at high cost, low efficiency, it is difficult to realize industrialized production, therefore current huperzine is main next
Still it is extracted from serrate clubmoss herb in source.Although serrate clubmoss herb wild resource is distributed in different regions, it is strongly fragrant to be grown in environment mostly
In the mountainous region thick forest that degree of closing is high, average annual rainfall is more, relative air humidity is big, slowly, the production cycle is long for breeding, from sprouting length
At intact plant it is generally necessary to 6~10 years.With the aggravation of aging of population, senile dementia takes place frequently, the demand of huperzine
Amount increases rapidly, causes serrate clubmoss herb wild resource by exhaustive exploitation, and the regeneration of serrate clubmoss herb plant resources receives very big limitation,
Therefore exploitation serrate clubmoss herb tissue culture technique is imperative.
Regulation of the plant tissue culture course by all Multi-environment factors such as illumination, temperature, humidity, wherein light is to plant
Growth and differentiation have extremely important effect, influence the whole process of Plant Tissue Breeding, traditional tissue culture light source is main
It is white light, the research of light organization of regulation control culture plant growth and development has focused largely on light intensity and light application time, and light quality is to plant
The research that object tissue cultures influence is less.Different light medium is different to the effect of different Plant Tissue Breeding, and reason may be plant
There are diversity for the chromophore of internal phytochrome molecules and apoprotein, and making phytochrome, there are different work in plant
Character state causes different tissues culture plant to show different reactions to light quality in terms of proliferative induction.It is trained in plant tissue
The more monochromatic light of application is feux rouges and blue light in supporting, and feux rouges can increase cell dry matter, is conducive to the formation of root, and blue light can be with
Make the plastid transformation chloroplaset in plant cell, has apparent facilitation to tissue culture plant tufted seedling proliferative induction.
Serine participates in a variety of important cell metabolisms in plant, and external source L- is added in Plant Tissue Breeding
Propylhomoserin can promote the differentiation of tissue culture plant Multiple Buds, and Serine or a kind of expression of adjusting Plant Light vrg genes
Semiochemicals, in the medium add Serine can cooperate with promote photoinduction proliferation function.
Serrate clubmoss herb tissue cultures are due to low and be difficult to obtain a large amount of two aspect factors of aseptic explant by breeding coefficient
It restricts, is not carried out large-scale production so far, the present invention is by combining factor of both light quality and medium component, it is intended to mention
For a kind of serrate clubmoss herb method for tissue culture suitable for large-scale production.
Summary of the invention
The present invention chooses the stem section close to root based on the division propagation ability that serrate clubmoss herb Wild plant itself has
As explant, screens suitable culture medium and carry out tissue cultures, by adding exogenous plant hormones, external source L- in the medium
Serine, while enhancing the intensity of illumination of blue monochromatic light during the cultivation process, reach raising serrate clubmoss herb explant tufted seedling and lures
The effect of conductance and tufted seedling breeding coefficient, and be proliferated by circulation-induced, a large amount of aseptic explants can be obtained, realize number
Amount quickly amplification, for serrate clubmoss herb plant resources protection and scale utilize new way is provided.
A kind of method concrete operation step improving serrate clubmoss herb tufted seedling breeding coefficient is as follows：
(1) wild serrate clubmoss herb sporinite surface sterilizing.The wild serrate clubmoss herb sporinite of stalwartness for choosing 10~15cm of plant height,
Dish washing liquid is dipped with banister brush in flowing water to clean branches and leaves and root, is rinsed well after dish washing liquid remained on surface ultra-clean
5~10min, 2% hypochlorous acid successively are impregnated with 75% 0.5~1.0min of alcohol solution dipping, 0.1% mercuric chloride solution in workbench
Sodium solution impregnates 15~20min, carries out surface sterilizing processing to sporinite, finally uses aseptic water washing 3~5 times, use aseptic paper
Blot surface moisture.
(2) explant is prepared.It is 2~3cm, with 6~10 that sporinite, which is aseptically cut, close to root, length
For the stem section of leaf as explant, explant upper cut-out is flat nick, and lower cut-out is 45° angle angular cut.
(3) explant adaptability culture.By explant in modified MS medium adaptability culture 2~3 weeks, selection does not have
Microbiological contamination, glossy, the not damaged hickie of base of leaf the explant of green, as surface sterile explant.Wherein, modified MS medium is matched
Fang Wei：1/4MS mineral element+1/2MS organic element+25g/L sucrose+6g/L agar, pH5.8~6.0；Condition of culture is：Temperature
25 ± 2 DEG C of degree, incandescent light 1000~1500Lx of intensity, 12~14h of light application time.
(4) explant endophyte sterilizes.Surface sterile explant is aseptically transferred to sterilize into endophyte and is cultivated
It is cultivated 4~5 weeks in base, chooses the explant that no microbiological contamination, cauline leaf is glossy, moisture is full, as aseptic explant.Wherein,
Endophyte sterilising medium formula is：Modified MS medium+0.3mg/L malachite green+40mg/L penicillin+50mg/L streptomysin
+ 100 μ g/L amphotericin Bs；Condition of culture is：25 ± 2 DEG C of temperature, incandescent light 1500~2000Lx of intensity, light application time 12
~14h.
(5) tufted seedling proliferative induction culture.Aseptic explant is aseptically transferred and is trained into tufted seedling proliferative induction
It supports and carries out tufted seedling proliferative induction in base, after culture 5~6 months, have 90% or more aseptic explant that can successfully induce and grow thickly
Seedling, each aseptic explant can produce 60~70 tufted seedlings.Wherein, tufted seedling proliferative induction culture medium prescription is：Improve MS
Culture medium+50~150mg/L+0.2~0.5mg/L of Serine 6-BA；Condition of culture is：25 ± 2 DEG C of temperature, illumination uses
Incandescent light+ultra-blue-light, total light intensity degree are 2000~2500Lx (wherein, 1000~1500Lx of incandescent light intensity, wavelength
450~480nm blue light strength, 500~1000Lx), 14~16h of light application time.
(6) tufted seedling grown cultures.By the tufted seedling of induction, aseptically whole clump turns access tufted seedling grown cultures
Cultivated 5~6 months in base, obtain sterile tufted seedling, 5~15cm of sterile tufted seedling height, partial bundle lower part with length 0.1~
The Hairy root of 0.5cm.Wherein, tufted seedling growth medium is：Modified MS medium+0.1~0.5mg/L NAA；Condition of culture
For：25 ± 2 DEG C of temperature, incandescent light 1500~2000Lx of intensity, 12~14h of light application time.
(7) circulation-induced is proliferated.The sterile tufted seedling that culture obtains is separated into single plant, a portion single plant is according to step
Suddenly the method for (2) prepares aseptic explant, continues the operation of step (5), (6), (7), carries out the circulation-induced proliferation of tufted seedling,
Another part single plant carries out root induction culture, grows up to complete serrate clubmoss herb aseptic plant.
Malachite green described in step (4), penicillin, streptomysin, amphotericin B solution are ready-to-use, cross and filter out
It is added after bacterium.
Blue light source described in step (5) is ultra-blue-light LED light.
Step (3), (4), (5), inoculum concentration is 60~80mL inoculation of medium 6~8 described in (6), inoculation
Direction is identical as plant natural growing direction, upright to cultivate.
Advantageous effects of the invention are embodied in the following aspects
(1) blue light and Serine all have facilitation, and Serine to Plant Tissue Breeding proliferative induction
It is a kind of plant control of photorespiration substance, blue light and Serine synergistic effect can further increase sterile to serrate clubmoss herb outer
The facilitation of implant tufted seedling proliferative induction.The present invention enhances indigo plant by adding external source Serine in the medium
The intensity of illumination of coloured light realizes serrate clubmoss herb aseptic explant tufted seedling induction success rate in 90% or more, averagely breeding coefficient
Reach 60 or more, i.e. a serrate clubmoss herb aseptic explant can induce the sterile tufted seedling of generation 60 or more, with natural conditions
Under compare, breeding coefficient significantly improves.
(2) serrate clubmoss herb is the pteridophyte with endophytic fungus, and wild serrate clubmoss herb sporinite will not only carry out surface disinfection,
And to kill endophyte and just be able to achieve sterile culture, sterilization-intensity is excessive to be easy to damage tissue, and it is too small, it is not achieved
How sterilization effect improves sterilizing success rate, obtains a large amount of sterilizings thoroughly and the impregnable aseptic explant of growth is to realize
One of the main restricting factor of serrate clubmoss herb large-scale production.The present invention only needs to carry out a disinfecting action, by proliferative induction
Culture obtains sterile tufted seedling, directly prepares aseptic explant with sterile tufted seedling, is proliferated by circulation-induced, can obtain big
Aseptic explant is measured, without repeating disinfecting action, improves working efficiency, simplification of flowsheet meets large-scale production requirement.
Detailed description of the invention
Fig. 1 is a kind of method and process process for improving serrate clubmoss herb tufted seedling breeding coefficient of the present invention.
Specific embodiment
Below with reference to specific embodiment, the present invention will be further described.The features and advantages of the invention will be with retouching
It states and becomes apparent from, but these exemplary embodiments are used merely to illustrate the present invention, the scope of the present invention is not constituted any
Limitation.
Embodiment 1：Comparison 6-BA, 6-BA+L- serine, 6-BA+ blue light, 6-BA+L- serine+four kinds of blue light difference lure
Influence of the conducting bar part to aseptic explant tufted seedling inductivity and tufted seedling breeding coefficient.
(1) wild serrate clubmoss herb sporinite surface sterilizing.The wild serrate clubmoss herb sporinite of stalwartness for choosing plant height 12cm or so,
Dish washing liquid is dipped with banister brush in flowing water to clean branches and leaves and root, is rinsed well after dish washing liquid remained on surface ultra-clean
8min successively is impregnated with 75% alcohol solution dipping 1.0min, 0.1% mercuric chloride solution in workbench, 2% liquor natrii hypochloritis soaks
20min is steeped, surface sterilizing processing is carried out to sporinite, finally uses aseptic water washing 5 times, blots surface moisture with aseptic paper.
(2) explant is prepared.Aseptically cut sporinite close to root, length 2.5cm, with 8 leaves
Stem section as explant, explant upper cut-out is flat nick, and lower cut-out is 45° angle angular cut.
(3) explant adaptability culture.By explant, adaptability culture 3 weeks, reservation do not contaminate in modified MS medium
Bacterium, glossy, the not damaged hickie of base of leaf the surface sterile explant of green.Wherein, modified MS medium formula is：1/4MS mine
Prime element+1/2MS organic element+25g/L sucrose+6g/L agar, pH5.8~6.0；Condition of culture is：It is 25 ± 2 DEG C of temperature, white
Vehement intensity of light 1200Lx, light application time 12h.
(4) explant endophyte sterilizes.Surface sterile explant is aseptically transferred to sterilize into endophyte and is cultivated
It is cultivated 5 weeks in base, retains the aseptic explant that no microbiological contamination, cauline leaf is glossy, moisture is full.Wherein, endophyte sterilizing culture
Based formulas is：Modified MS medium+0.3mg/L malachite green+40mg/L penicillin+50mg/L+100 μ g/L both sexes of streptomysin are mould
Plain B；Condition of culture is：25 ± 2 DEG C of temperature, incandescent light intensity 1500Lx, light application time 12h.
(5) tufted seedling proliferative induction culture.Aseptic explant is aseptically transferred and is trained into tufted seedling proliferative induction
It supports and carries out tufted seedling proliferative induction in base, after culture 6 months, observe 6-BA, 6-BA+L- serine, 6-BA+ blue light, 6-BA+L-
Influence of four kinds of the serine+blue light different inductive conditions to aseptic explant tufted seedling inductivity and tufted seedling breeding coefficient.Its
In, culture medium basis is modified MS medium；Condition of culture is：25 ± 2 DEG C of temperature, total light intensity degree is 2500Lx, illumination
Time 14h.
(6) tufted seedling grown cultures.By the tufted seedling of induction, aseptically whole clump turns access tufted seedling grown cultures
It is cultivated 6 months in base, obtains sterile tufted seedling.Wherein, tufted seedling growth medium is：Modified MS medium+0.5mg/L
NAA；Condition of culture is：25 ± 2 DEG C of temperature, incandescent light intensity 1500Lx, light application time 12h.
Malachite green, penicillin, streptomysin, amphotericin B solution are ready-to-use, add after equal filtration sterilization；Blue light light
Source is ultra-blue-light LED light；Inoculum concentration is 60mL inoculation of medium 6, is inoculated with direction and plant natural growing direction phase
Together, upright culture.
Influence of the different inductive conditions to serrate clubmoss herb tufted seedling inductivity and tufted seedling breeding coefficient
Note：Different lowercase letter indication differences is significant, p < 0.05.
Embodiment 2：Compare the tufted seedling inductivity of wild sporinite source explant and sterile tufted seedling source explant
With tufted seedling breeding coefficient.
(1) prepared by wild sporinite source explant.The wild sporinite of healthy and strong serrate clubmoss herb for choosing plant height 12cm or so, is pressed
According to the technical parameter in embodiment 1, through surface sterilizing, explant, the culture of explant adaptability, the sterilizing of explant endophyte are prepared
Etc. operating procedures, prepare wild sporinite source aseptic explant.
(2) prepared by sterile tufted seedling source aseptic explant.The healthy and strong serrate clubmoss herb of selection plant height 12cm or so is sterile to grow thickly
Seedling is aseptically cut close to root, length 2.5cm, the stem section with 8 leaves as explant, on explant
Portion's notch is flat nick, and lower cut-out is 45° angle angular cut, prepares sterile tufted seedling source explant.
(3) tufted seedling proliferative induction culture.Separate sources aseptic explant is aseptically transferred and is lured into tufted seedling
Progress tufted seedling proliferative induction in proliferated culture medium is led, after culture 6 months, observation separate sources aseptic explant tufted seedling induction
Rate and tufted seedling breeding coefficient.Wherein, tufted seedling Fiber differentiation based formulas is：Modified MS medium+100mg/L Serine+
0.5mg/L 6-BA；Condition of culture is：25 ± 2 DEG C of temperature, illumination uses incandescent light+ultra-blue-light, and total light intensity degree is
2500Lx (wherein, incandescent light intensity 1500Lx, 450~480nm of wavelength blue light strength 1000Lx), light application time 14h.
(4) tufted seedling grown cultures.By the tufted seedling of induction, aseptically whole clump turns access tufted seedling grown cultures
It is cultivated 6 months in base, obtains sterile tufted seedling.Wherein, tufted seedling growth medium is：Modified MS medium+0.5mg/L
NAA；Condition of culture is：25 ± 2 DEG C of temperature, incandescent light intensity 1500Lx, light application time 12h.
Malachite green, penicillin, streptomysin, amphotericin B solution are ready-to-use, add after equal filtration sterilization；Blue light light
Source is ultra-blue-light LED light；Inoculum concentration is 60mL inoculation of medium 6, is inoculated with direction and plant natural growing direction phase
Together, upright culture.
Separate sources explant tufted seedling inductivity and tufted seedling breeding coefficient
Note：The equal indifference of above two groups of test results is significant, p < 0.05.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvements and modifications, these improvements and modifications can also be made
Also it should be regarded as protection scope of the present invention.
Claims (4)
1. a kind of method for improving serrate clubmoss herb tufted seedling breeding coefficient, it is characterised in that include the following steps：
(1) wild serrate clubmoss herb sporinite surface sterilizing.The wild serrate clubmoss herb sporinite of stalwartness for choosing 10~15cm of plant height, in flowing water
It is middle to dip dish washing liquid with banister brush branches and leaves and root are cleaned, it rinses well after dish washing liquid remained on surface in ultra-clean work
It is successively molten with 75% 0.5~1.0min of alcohol solution dipping, 0.1% mercuric chloride solution immersion, 5~10min, 2% sodium hypochlorite in platform
Liquid impregnates 15~20min, carries out surface sterilizing processing to sporinite, finally uses aseptic water washing 3~5 times, blotted with aseptic paper
Surface moisture.
(2) explant is prepared.It is 2~3cm, with 6~10 leaves that sporinite, which is aseptically cut, close to root, length
Stem section as explant, explant upper cut-out is flat nick, and lower cut-out is 45° angle angular cut.
(3) explant adaptability culture.By explant, adaptability culture 2~3 weeks, selection do not contaminate in modified MS medium
Bacterium, glossy, the not damaged hickie of base of leaf the explant of green, as surface sterile explant.Wherein, modified MS medium formula
For：1/4MS mineral element+1/2MS organic element+25g/L sucrose+6g/L agar, pH5.8~6.0；Condition of culture is：Temperature
25 ± 2 DEG C, incandescent light 1000~1500Lx of intensity, 12~14h of light application time.
(4) explant endophyte sterilizes.Surface sterile explant is aseptically transferred in endophyte sterilising medium
The explant that no microbiological contamination, cauline leaf is glossy, moisture is full, as aseptic explant are chosen in culture 4~5 weeks.Wherein, interior life
Bacterium sterilising medium formula is：Modified MS medium+0.3mg/L malachite green+40mg/L penicillin+50mg/L streptomysin+100
μ g/L amphotericin B；Condition of culture is：25 ± 2 DEG C of temperature, incandescent light 1500~2000Lx of intensity, light application time 12~
14h。
(5) tufted seedling proliferative induction culture.Aseptic explant is aseptically transferred into tufted seedling proliferative induction culture medium
Middle progress tufted seedling proliferative induction has 90% or more aseptic explant that can successfully induce tufted seedling, often after culture 5~6 months
A aseptic explant can produce 60~70 tufted seedlings.Wherein, tufted seedling proliferative induction culture medium prescription is：Modified MS medium
+ 0.2~0.5mg/L6-BA of+50~150mg/LL- serine；Condition of culture is：25 ± 2 DEG C of temperature, illumination uses incandescent light
+ ultra-blue-light, total light intensity degree are 2000~2500Lx (wherein, 1000~1500Lx of incandescent light intensity, 450~480nm of wavelength
500~1000Lx of blue light strength), 14~16h of light application time.
(6) tufted seedling grown cultures.By the tufted seedling of induction, aseptically whole clump turns in access tufted seedling growth medium
Culture 5~6 months, obtains sterile tufted seedling, 5~15cm of sterile tufted seedling height, partial bundle lower part with length 0.1~
The Hairy root of 0.5cm.Wherein, tufted seedling growth medium is：Modified MS medium+0.1~0.5mg/LNAA；Condition of culture
For：25 ± 2 DEG C of temperature, incandescent light 1500~2000Lx of intensity, 12~14h of light application time.
(7) circulation-induced is proliferated.The sterile tufted seedling that culture obtains is separated into single plant, a portion single plant is according to step
(2) method prepares aseptic explant, continues the operation of step (5), (6), (7), carries out the circulation-induced proliferation of tufted seedling, separately
A part of single plant carries out root induction culture, grows up to complete serrate clubmoss herb aseptic plant.
2. a kind of method for improving serrate clubmoss herb tufted seedling breeding coefficient according to claim 1, it is characterised in that step (4)
Described in malachite green, penicillin, streptomysin, amphotericin B solution it is ready-to-use, added after equal filtration sterilization.
3. a kind of method for improving serrate clubmoss herb tufted seedling breeding coefficient according to claim 1, it is characterised in that step (5)
Described in blue light source be ultra-blue-light LED light.
4. a kind of method for improving serrate clubmoss herb tufted seedling breeding coefficient according to claim 1, it is characterised in that step
(3), (4), (5), inoculum concentration is 60~80mL inoculation of medium 6~8 described in (6), inoculation direction and plant from
The right direction of growth is identical, upright to cultivate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810552264.6A CN108925427B (en) | 2018-05-31 | 2018-05-31 | Method for improving propagation coefficient of huperzia serrata cluster seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810552264.6A CN108925427B (en) | 2018-05-31 | 2018-05-31 | Method for improving propagation coefficient of huperzia serrata cluster seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108925427A true CN108925427A (en) | 2018-12-04 |
| CN108925427B CN108925427B (en) | 2021-05-18 |
Family
ID=64449715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810552264.6A Active CN108925427B (en) | 2018-05-31 | 2018-05-31 | Method for improving propagation coefficient of huperzia serrata cluster seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108925427B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258425A (en) * | 2018-09-29 | 2019-01-25 | 西北大学 | A method of improving Determination of hupzine A in Huperzia serrata |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019487A (en) * | 2006-12-21 | 2007-08-22 | 浙江省中药研究所有限公司 | Imitating wild planting process of Chinese huperzine serrate |
| CN101889551A (en) * | 2010-08-06 | 2010-11-24 | 合肥工业大学 | Tissue culture method of huperizia serrata |
| JP2012065578A (en) * | 2010-09-22 | 2012-04-05 | Univ Of Tokyo | Production of tricyclic non-natural type alkaloid using vegetable polyketide synthase |
| CN102714991A (en) * | 2011-03-30 | 2012-10-10 | 广西壮族自治区药用植物园 | Lycopodium serratum cutting propagation method |
| CN105815218A (en) * | 2016-03-29 | 2016-08-03 | 西北大学 | Method for improving huperzia serrata explant surface sterilization efficiency |
| CN106613938A (en) * | 2016-09-09 | 2017-05-10 | 江西农业大学 | Effective huperzia serrata callus culture method |
-
2018
- 2018-05-31 CN CN201810552264.6A patent/CN108925427B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101019487A (en) * | 2006-12-21 | 2007-08-22 | 浙江省中药研究所有限公司 | Imitating wild planting process of Chinese huperzine serrate |
| CN101889551A (en) * | 2010-08-06 | 2010-11-24 | 合肥工业大学 | Tissue culture method of huperizia serrata |
| JP2012065578A (en) * | 2010-09-22 | 2012-04-05 | Univ Of Tokyo | Production of tricyclic non-natural type alkaloid using vegetable polyketide synthase |
| CN102714991A (en) * | 2011-03-30 | 2012-10-10 | 广西壮族自治区药用植物园 | Lycopodium serratum cutting propagation method |
| CN105815218A (en) * | 2016-03-29 | 2016-08-03 | 西北大学 | Method for improving huperzia serrata explant surface sterilization efficiency |
| CN106613938A (en) * | 2016-09-09 | 2017-05-10 | 江西农业大学 | Effective huperzia serrata callus culture method |
Non-Patent Citations (5)
| Title |
|---|
| 李晓君 等: "蛇足石杉茎尖组织培养研究", 《中国现代中药》 * |
| 杨雪飞 等: "蛇足石杉茎尖灭菌方法与组织培养的研究", 《安徽农业科学》 * |
| 王玉珍: "《现代植物组织培养原理及应用技术》", 31 January 2018, 中国原子能出版社 * |
| 王蒂 等: "《植物组织培养》", 31 August 2013, 中国农业出版社 * |
| 马英姿 等: "蛇足石杉的离体培养", 《植物生理学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258425A (en) * | 2018-09-29 | 2019-01-25 | 西北大学 | A method of improving Determination of hupzine A in Huperzia serrata |
| CN109258425B (en) * | 2018-09-29 | 2023-03-03 | 西北大学 | Method for increasing content of huperzine A in huperzia serrata |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108925427B (en) | 2021-05-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107347651A (en) | The cultural method that dove tree hibernaculum quickly breeds | |
| CN103380730B (en) | Tissue-culture rapid propagation method for pyrus betulaefolia bunge | |
| CN102144556A (en) | Method for tissue culture and rapid propagation of Dayaoshania cotinifolia W. T. Wang | |
| CN106489734B (en) | The breeding method of the nontoxic seedling of fortune paulownia | |
| CN103651124B (en) | A kind of abductive approach of plant regeneration of zingiber officinale | |
| CN104686361B (en) | The induction of a kind of Fructus Vitis viniferae embryo callus and cultural method | |
| CN107980634A (en) | A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation | |
| CN103688856A (en) | Rapid propagation method of Blumea riparia (Bl.) DC medicinal material | |
| CN108925427A (en) | A method of improving serrate clubmoss herb tufted seedling breeding coefficient | |
| CN108782247A (en) | A kind of method for tissue culture of late cherry " Yu Yihuang " kind of Japan | |
| CN102232359B (en) | In-vitro rapid propagation method of double-petal Jasminum sambac | |
| CN107568069B (en) | A kind of smoothbark birch tissue-cultured seedling high efficiently multiplying method | |
| CN108552055B (en) | A kind of powder leaf golden flower quick breeding method for tissue culture | |
| CN106069784A (en) | A kind of method for tissue culture of Flos Caryophylli Fructus Gardeniae petal | |
| CN105706924A (en) | Fast industrialized production method for ardisia violacea | |
| CN108633742A (en) | A kind of China fir Stem tip induction culture medium and abductive approach | |
| CN114902962A (en) | Living body intermittent disinfection method for clonal propagation of Epimedium sagittatum root tuber | |
| CN108271693A (en) | One kind posting tree orchid regenerating system quickly foundation and store method | |
| CN107372120A (en) | The cultural method that dove tree branch is quickly bred | |
| CN108077076A (en) | A kind of quick-breeding method of Ammopiptanthus mongolicus | |
| CN108094206A (en) | A kind of cultural method of cystoathyrium chinense sporinite breeding | |
| CN106665358A (en) | Rapid shoot induction and tissue culture propagation method for platycodon grandiflorum leaves | |
| CN102823446A (en) | Efficient method for sugarcane infection by smut spores | |
| CN108271687A (en) | A kind of method for tissue culture of butterfly cherry | |
| CN107372118B (en) | A kind of golden larch test tube seeding cultivating method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210409 Address after: Room 312, business park, 5F, business center, Hefei University Town, Hefei Economic and Technological Development Zone, Anhui 230001 Applicant after: HEFEI SHANZHIXIAN BIOTECHNOLOGY Co.,Ltd. Address before: 230088 phase II of Innovation Industrial Park, 2800 innovation Avenue, high tech Zone, Hefei City, Anhui Province Applicant before: HEFEI BENSHENG BIOTECHNOLOGY Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |