CN102972435B - Stem dormant bud germination accelerating agent and germination accelerating method thereof - Google Patents
Stem dormant bud germination accelerating agent and germination accelerating method thereof Download PDFInfo
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- 230000035784 germination Effects 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000005972 6-Benzyladenine Substances 0.000 claims abstract description 20
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 20
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 12
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003448 gibberellin Substances 0.000 claims abstract description 12
- 239000007864 aqueous solution Substances 0.000 claims abstract description 9
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 10
- ANHAEBWRQNIPEV-UHFFFAOYSA-N 2-chloroethyl dihydrogen phosphate Chemical compound OP(O)(=O)OCCCl ANHAEBWRQNIPEV-UHFFFAOYSA-N 0.000 claims description 9
- 208000027418 Wounds and injury Diseases 0.000 claims description 7
- 241000208140 Acer Species 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 241000927509 Heteropanax Species 0.000 claims description 5
- 230000006378 damage Effects 0.000 claims description 3
- 208000014674 injury Diseases 0.000 claims description 3
- 238000012958 reprocessing Methods 0.000 claims description 2
- 230000000472 traumatic effect Effects 0.000 claims description 2
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 abstract description 10
- 239000003795 chemical substances by application Substances 0.000 abstract description 6
- 239000005976 Ethephon Substances 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 29
- 230000011218 segmentation Effects 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 6
- 239000005648 plant growth regulator Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 229920000742 Cotton Polymers 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000005556 hormone Substances 0.000 description 5
- 229940088597 hormone Drugs 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 4
- 238000007405 data analysis Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000013517 stratification Methods 0.000 description 3
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 3
- 229920002554 vinyl polymer Polymers 0.000 description 3
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 2
- 241001423497 Himalayacalamus falconeri Species 0.000 description 2
- 241000632869 Pachira glabra Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 235000006214 castanha do maranho Nutrition 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000005059 dormancy Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
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- 235000013311 vegetables Nutrition 0.000 description 2
- MVXMNHYVCLMLDD-UHFFFAOYSA-N 4-methoxynaphthalene-1-carbaldehyde Chemical compound C1=CC=C2C(OC)=CC=C(C=O)C2=C1 MVXMNHYVCLMLDD-UHFFFAOYSA-N 0.000 description 1
- 241001677259 Acanthophoenix rubra Species 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- -1 GA 3 Chemical compound 0.000 description 1
- 244000302512 Momordica charantia Species 0.000 description 1
- 235000009811 Momordica charantia Nutrition 0.000 description 1
- 235000009812 Momordica cochinchinensis Nutrition 0.000 description 1
- 235000018365 Momordica dioica Nutrition 0.000 description 1
- 244000037795 Pachira macrocarpa Species 0.000 description 1
- 235000003283 Pachira macrocarpa Nutrition 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 230000028245 fruit abscission Effects 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- JLJLRLWOEMWYQK-GDUNQVSHSA-N giberellic acid Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)C1C(O)=O)CC2[C@@]2(OC3=O)C1[C@]3(C)[C@@H](O)CC2 JLJLRLWOEMWYQK-GDUNQVSHSA-N 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
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Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a stem dormant bud germination accelerating agent and a germination accelerating method for stem dormant buds by adopting the stem dormant bud germination accelerating agent. The stem dormant bud germination accelerating agent comprises an aqueous solution with the following components in concentration: 10-30 mg/L of N6-furylmethyl adenine (KT), 50-200 mg/L of 6-benzyl adenine (6-BA), Gibberellin (GA)3) 300-1000 mg/L, 200-400 mg/L2-chloroethyl phosphonic acid (ethephon). The stem dormant bud germination accelerating agent and the germination accelerating method for the stem dormant buds by adopting the stem dormant bud germination accelerating agent can overcome the defects of the prior art, promote dormant bud germination, enable dormant bud germination at a specified position, ensure the germination quantity and cultivate potted plants with good tree crowns.
Description
Technical field
The present invention relates to a kind of stem sleeping bud germinator, and the germination accelerating method adopting this stem sleeping bud germinator to carry out stem sleeping bud.
Technical background
Stem segmentation pot plant to refer to plant trunk stem section as viewing and admiring, trade, object of transport flowers product form.Stem section product, after reaching consumption destination, is germinateed by process stem section hestening rooting, is formed and finally view and admire potted plant.Stem segmentation is potted plant, also can be called that stem segmentation cuts branch.At present on flowers market, common stem segmentation pot flowers has Brazilian iron (Brazilian rod), Dutch iron, Malabar chestnut (money tree), fairy bamboo, great Ye umbrella and heavy curtain umbrella maple etc.Stem segmentation pot flowers mainly money tree and the fairy bamboo of China's export, outlet history is longer.The great Ye umbrella of nearest exploitation, heavy curtain umbrella maple, green precious market prospects are tempting.Plant stem sleeping bud, to refer on plant stem inactive axillalry bud in the season of growth, temporarily keeps resting state, be called sleeping bud.
Plant growth regulator is also called plant exogenous hormone, is the method that people pass through chemistry, and the chemical constitution of copying plant hormone similar or physiological action, synthesis has the material of physiologically active.It is multiple that plant growth regulator comprises cytokinin, gibberellin class, vinyl etc., and in plant husbandry, application is very wide, plays important regulating action to growth and development of plants.Cell division have and promotes cell division and differentiation, and promote lateral bud growth and the remarkable feature changing other hormonal actions, conventional its active ingredient of kinetin KT(that has is N6-furfuryladenine) and 6-BA(6-benzyladenine).Gibberellin can promote that axis, leaf grow, and break the dormancy of some organ of plant corpus significantly, promotes that long-day plant blooms, can increase melon female flower number, induced parthenocarpy, and improve fruit-setting rate etc., people are serial by the precedence called after GA found, as GA
3.What vinyl was with the most use is ethrel (its active ingredient is 2 chloroethyl phosphoric acid), and it produces ethene in metabolic process.Ethene have promote fruit, blade and plant as early as possible ripe, T suppression cell elongation growth, promote the differentiation of leaf, flower, fruit abscission, floral bud induction and promote to occur the effect of adventive root.When breaking the dormancy of plant stem bud, the combination of different classes of plant growth regulator plays different effects, for improving germination quantity, speed and germination quality etc., needs the preferred compositions exploring each parahormone.
Modern Stratification technique many employings plant growth regulator carries out vernalization.On vernalization material, mainly concentrate on the vernalization aspect of seed and branch, nursery stock, such as at the Side bud inducement (Zhou Honglong etc. of flowers as the red palm, north gardening, 2011(02): 144-145), vegetables are as the breaking (You Yawei etc., North China agronomy report, 2007(22(supplementary issue) for seed domant bud such as celery, balsam pear: 61-63; From superfine, China's Vegetable, 2010(14): 60-63) and fruit tree field such as apple nursery stock, branch branch situation (Zan swallow etc., Journal of Northwest Sci Tech University of Agriculture and Forestry (natural science edition), 2011,39(6): 185-190; Sun Qingwei etc., Journal of Fruit Science, 2011,28(6): 1071-1076; Liu Zhi etc., northern fruit tree, 1999(2): 11,19), fruit shapes etc. (Sun Xisheng etc., northern fruit tree, 1997(2): application 10-13).In the selection of vernalization hormone, adopt cytokinin, gibberellin class, vinyl, as general Lip river Malin, send out branch element, 6-BA, GA3, calcium cyanamide, ethrel etc., it is comparatively common that multiple hormone mixes situation about using more.In the concentration application of germinator, the adaptation of different plant to concentration is different, generally presents the trend of " seed < flowers < nursery stock ".In the application mode of Stratification technique, have plenty of and directly smear plant growth regulator, some is then the mode of smearing conditioning agent after first carving wound.
As can be seen here, the germination of existing stem segmentation pot plant is spontaneous germination, and artificial adjustment is few, less effective.Rare sleeping bud vernalization plant growth regulator combination being used for stem segmentation pot plant stem at present, its best of breed composition, the most proper combination concentration wait for exploration, and Stratification technique awaits development is applied.Due to stem section the later stage germinate take root in there is certain uncertain factor, quantity, the position of germination, the speed of germination as germinateed all affect the efficiency of production and the quality of product.Germinate few, tree crown is not plentiful; Germination position is bad, causes inclined hat, tree-like difference; Speed of germination is slow, and cause curing time long, cost is high.
In view of this, developing a kind of novel stem sleeping bud germinator and overcome above-mentioned the deficiencies in the prior art, can promote that sleeping bud is sent out soon, assigned address is sprouted, ensure germination quantity, cultivating good potted plant of tree crown is those skilled in the art's technical problems urgently to be resolved hurrily.
Summary of the invention
For the deficiencies in the prior art, technical problem solved by the invention is the germination accelerating method providing a kind of stem sleeping bud germinator and adopt this stem sleeping bud germinator to carry out stem sleeping bud, can the deficiencies in the prior art be overcome, can promote that sleeping bud is sent out soon, assigned address is sprouted, ensure germination quantity, cultivate good potted plant of tree crown.
For this reason, on the one hand, a kind of stem sleeping bud germinator provided by the invention, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine (KT) 10 ~ 30mg/L,
6-benzyladenine (6-BA) 50 ~ 200mg/L,
Gibberellin (GA
3) 300 ~ 1000mg/L,
2 chloroethyl phosphoric acid (ethrel) 200 ~ 400mg/L.
Preferably, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine (KT) 15 ~ 23mg/L,
6-benzyladenine (6-BA) 80 ~ 130mg/L,
Gibberellin (GA
3) 450 ~ 530mg/L,
2 chloroethyl phosphoric acid (ethrel) 260 ~ 320mg/L.
Further preferably, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine (KT) 20mg/L,
6-benzyladenine (6-BA) 100mg/L,
Gibberellin (GA
3) 500mg/L,
2 chloroethyl phosphoric acid (ethrel) 300mg/L.
Wherein, described stem sleeping bud is the stem sleeping bud of Araliaceae Ivy Tree Bark genus and Araliaceae Heteropanax seeds.
Specifically, described stem sleeping bud is the stem sleeping bud of great Ye umbrella and heavy curtain umbrella maple.
On the other hand, technical problem solved by the invention is the germination accelerating method providing a kind of stem sleeping bud, and be included in the surface coating stem sleeping bud germinator of stem sleeping bud, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine (KT) 10 ~ 30mg/L,
6-benzyladenine (6-BA) 50 ~ 200mg/L,
Gibberellin (GA
3) 300 ~ 1000mg/L,
2 chloroethyl phosphoric acid (ethrel) 200 ~ 400mg/L;
The coating step of this stem sleeping bud germinator comprises:
1-2cm place above stem sleeping bud carries out injury reason at quarter to it, carves with a knife wound to the bast of stem, and more than xylem, then stem sleeping bud germinator described in stem sleeping bud surface and position, traumatic part coating at quarter 1-2mL;
Complete above-mentioned process after 5 ~ 7 days, reprocessing 1-2 time according to the method described above.
Preferably, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine (KT) 15 ~ 23mg/L,
6-benzyladenine (6-BA) 80 ~ 130mg/L,
Gibberellin (GA
3) 450 ~ 530mg/L,
2 chloroethyl phosphoric acid (ethrel) 260 ~ 320mg/L.
Further preferably, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine (KT) 20mg/L,
6-benzyladenine (6-BA) 100mg/L,
Gibberellin (GA
3) 500mg/L,
2 chloroethyl phosphoric acid (ethrel) 300mg/L.
Moreover, present invention also describes stem sleeping bud germinator of the present invention, Araliaceae Ivy Tree Bark belong to and Araliaceae Heteropanax arbor stem sleeping bud vernalization process in application.
Specifically, stem sleeping bud germinator of the present invention is the application in the stem sleeping bud vernalization process of great Ye umbrella and heavy curtain umbrella maple.
Compared to prior art, stem sleeping bud germinator of the present invention and the germination accelerating method adopting this stem sleeping bud germinator to carry out stem sleeping bud, can the deficiencies in the prior art be overcome, promote Dormant Buds, assigned address Dormant Buds, ensure germination quantity, cultivate good potted plant of tree crown.
Embodiment
Embodiment one:
The present embodiment explores the hormone kind of vernalization and concentration, mainly selects 6-BA, GA
3, ethrel, be applied in the sleeping bud of great Ye umbrella stem section, found suitable hormone combinations kind and concentration.
Test is treated to: by the recipe configuration solution of table 1.First time on August 15th, 2011 applies vernalization, and August 21 pasted cotton vernalization again, and day entry in September 4 just secondary data, lay equal stress on and brush one times each formula, September 13 recorded data again.12 formulas are set, the strain of each formula 3, strain 5 bud, altogether 15 buds.One strain has 4 process of filling a prescription, totally 20 buds.Each formula has 60 buds, 12 formula total 720 buds.
The setting (unit: mg/L) of 12, table 1 formula
Data record, to be analyzed as follows:
(1) September 4 data
The effect record (unit: individual) of 12, table 2 formula to the vernalization of plant sleeping bud
Indicate: number 1-12 numeral 12 formula above in table, after (1) (2) (3) expression 3 experimental plants numbering.
Do not list without the plant germinateed.
The data analysis (unit: individual) of 12, table 3 formula to the vernalization of plant sleeping bud
(2) September 13 data
The effect record (unit: individual) of 12, table 4 formula to the vernalization of plant sleeping bud
Indicate: number 1-12 numeral 12 formula above in table, after (1) (2) (3) expression 3 experimental plants numbering.
Do not list without the plant germinateed.
The data analysis (unit: individual) of 12, table 5 formula to the vernalization of plant sleeping bud
Data analysis from above-mentioned test: carry out graduating with cutter process than graduating with cutter vernalization rate is not high to material, illustrates that carving wound can promote Dormant Buds, therefrom selects optimization formula 3 and formula 9.
Embodiment two:
The present embodiment is the further exploration to formula concentration, and adds KT, by carving the injury reason preferred formula of research vernalization rate further on the basis of embodiment one.
Test is treated to: by the recipe configuration solution of table 6.On September 19th, 2011 carries out test of accelerating, October 15 day entry data.Totally 8 formulas, the strain of each formula 3, every strain process 10 buds, 5 graduating with cutters do not wrap cotton, and 5 graduating with cutter bags are cotton.
The new formula (unit: mg ∕ L) of table 6 germinator test
The data record that 8, table 7 is newly filled a prescription to the vernalization of plant sleeping bud
The data statistics that 8, table 8 is newly filled a prescription to the vernalization of plant sleeping bud
Learn: the Germination effect of formula 3 and formula 8 pairs of stem sleeping buds is best, in processing procedure, have cotton and without cotton, its vernalization rate affected not quite by testing above.Present invention further optimization scheme is formula 3, and most preferably scheme is formula 8.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (5)
1. a stem sleeping bud germinator, is characterized in that, belong to and Araliaceae Heteropanax seeds for Araliaceae Ivy Tree Bark, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine 20mg/L,
6-benzyladenine 100mg/L,
Gibberellin 500 mg/L,
2 chloroethyl phosphoric acid 300mg/L.
2. stem sleeping bud germinator according to claim 1, is characterized in that: described stem sleeping bud is the stem sleeping bud of great Ye umbrella and heavy curtain umbrella maple.
3. a germination accelerating method for stem sleeping bud, is characterized in that, be included in the surface coating stem sleeping bud germinator of stem sleeping bud, this stem sleeping bud germinator is the aqueous solution comprising following concentration components:
N6-furfuryladenine 20mg/L,
6-benzyladenine 100mg/L,
Gibberellin 500 mg/L,
2 chloroethyl phosphoric acid 300mg/L;
The coating step of this stem sleeping bud germinator comprises:
Araliaceae Ivy Tree Bark belong to and Araliaceae Heteropanax seeds stem sleeping bud above 1-2cm place to its carry out quarter injury manage, carve with a knife wound to the bast of stem, more than xylem, then stem sleeping bud germinator described in stem sleeping bud surface and position, traumatic part coating at quarter 1-2mL;
Complete above-mentioned process after 5 ~ 7 days, reprocessing 1-2 time according to the method described above.
4. the germination accelerating method of stem sleeping bud as claimed in claim 3, Araliaceae Ivy Tree Bark belong to and Araliaceae Heteropanax arbor stem sleeping bud vernalization process in application.
5. the germination accelerating method of stem sleeping bud as claimed in claim 4, the application in the stem sleeping bud vernalization process of great Ye umbrella and heavy curtain umbrella maple.
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| CN103999594B (en) * | 2014-05-29 | 2016-05-04 | 兰州大学 | A kind of economy, abolish husky rice seed dormancy and promote the method for its sprouting fast |
| CN104541660A (en) * | 2014-12-24 | 2015-04-29 | 陇西千金药材有限公司 | Method for expediting dormant bud of radix codonopsis by chemical treatment method |
| CN105557438B (en) * | 2016-01-21 | 2018-06-12 | 杭州润土园艺科技有限公司 | A kind of increase ilex verticillata cuts a method for result side shoot quantity |
| CN105830839B (en) * | 2016-03-25 | 2019-02-19 | 中国科学院合肥物质科学研究院 | A method of tung oil tree fruit yield is improved by hormone induction |
| CN106069023A (en) * | 2016-06-17 | 2016-11-09 | 广西滨地生态农业投资有限责任公司 | A kind of method of HUOLONG fruit dot bud flower forcing |
| CN107182357A (en) * | 2017-07-18 | 2017-09-22 | 合肥百绿盛农业科技有限公司 | A kind of Pregermination and seedling breeding method of jujube seed |
| CN107372111B (en) * | 2017-08-03 | 2019-07-23 | 湖北理工学院 | A kind of celery artificial seed and its fast preparation method |
| CN109511457A (en) * | 2018-12-11 | 2019-03-26 | 广西贺州市正丰现代农业股份有限公司 | Grape breaks economise-create and hurts germination accelerating method and the grape cultivation method based on the germination accelerating method |
| CN113966744B (en) * | 2021-10-20 | 2023-04-21 | 上海市农业科学院 | Plant growth regulator, application thereof and method for regulating grape growth |
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