CN107751224B - Seed soaking liquid and method for improving germination rate of aged peanut seeds - Google Patents
Seed soaking liquid and method for improving germination rate of aged peanut seeds Download PDFInfo
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- CN107751224B CN107751224B CN201711172677.3A CN201711172677A CN107751224B CN 107751224 B CN107751224 B CN 107751224B CN 201711172677 A CN201711172677 A CN 201711172677A CN 107751224 B CN107751224 B CN 107751224B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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Abstract
The invention relates to a seed soaking liquid for improving the germination rate of aged peanut seeds, which consists of the following components in percentage by weight: 100-300mg/L gibberellin GA30.1-0.3mg/L brassinolide, 0.1-0.3mg/L ethenol, 0.1-0.3mg/L cytokinin 6-BA, 0.2-0.4mg/L auxin IAA and the balance of water. The invention also provides a method for improving the germination rate of aged peanut seeds, which comprises the following steps: s1, preprocessing: airing the aged peanut seeds; s2, preparing seed soaking liquid: gibberellin GA3100-300mg/L of brassinolide, 0.1-0.3mg/L of ethylene, 0.1-0.3mg/L of cytokinin and 0.2-0.4mg/L of auxin IAA; s3, seed soaking: soaking the peanut seeds in warm water for half an hour, then placing the peanut seeds into a seed soaking solution for soaking at normal temperature, and then taking out the peanut seeds for sowing.
Description
Technical Field
The invention relates to the field of agricultural planting, in particular to seed soaking liquid and a method for improving the germination rate of aged peanut seeds.
Background
Peanut (Arachis Hypogaea L.) is one of four major oil crops in the world, and is also an important oil and economic crop in China. The total output, consumption and export of peanuts in China are the top of the world for a long time, so that the peanuts have a very important position in agricultural economy in China.
Peanut seeds are easy to age in the process of storage at normal temperature, so that the vitality of the seeds is reduced, and the germination rate is reduced. Therefore, the improvement of the germination rate of the peanut seeds, the acceleration of the seed germination and the promotion of the seed emergence become key links for ensuring the high and stable yield of the peanuts. Currently, researches on how to improve the germination rate of peanut seeds are few, and farmers generally adopt a warm soup seed soaking method to accelerate germination of peanut seeds. The main method is that the peanut seeds are immersed in warm water with the temperature of 50-55 ℃ for 6-12 hours to ensure that the seeds absorb enough water, then the water is discharged, the seeds are wrapped by gauze and put in a pot for accelerating germination. Although the warm soup seed soaking method is simple, convenient and quick, and has low cost, a plurality of problems also exist, including low seed germination rate, slow seedling emergence, irregular seedling emergence and serious seedling size phenomenon. Recently, studies have been made on accelerating germination of peanut seeds after the peanut seeds are soaked in gibberellin solution for 8 hours, so that the germination rate can be improved by 5% -8%, but the improvement effect on the germination rate is not obvious on the whole, and particularly, the improvement on the germination rate of aged peanut seeds with low germination rate by 5% -8% is not significant. Therefore, how to develop a method for treating aged peanut seeds with high germination rate has important practical significance.
Disclosure of Invention
The invention aims to solve the technical problem of providing a seed soaking liquid and a method for improving the germination rate of aged peanut seeds, which can obviously enhance the vitality of the aged peanut seeds, improve the germination rate and the fresh and alive quality of sprouts. In addition, the method can obviously improve the germination rate of aged peanut seeds, and can also remove dormancy of fresh peanut seeds, thereby improving the germination rate.
In order to solve the technical problems, the invention adopts the following technical scheme:
a seed soaking liquid for improving the germination rate of aged peanut seeds comprises the following components in percentage by weight:
gibberellin GA3100 mg/L, 0.1-0.3mg/L of brassinolide, 0.1-0.3mg/L of ethylene, 0.1-0.3mg/L of cytokinin, 0.2-0.4mg/L of auxin IAA and the balance of water.
Preferably, the seed soaking liquid for improving the germination rate of the aged peanut seeds consists of the following components in percentage by weight: gibberellin GA3200mg/L, 0.2mg/L of brassinolide, 0.1mg/L of ethylene, 0.1mg/L of cytokinin 6-BA, 0.2mg/L of auxin IAA and the balance of water.
A method for improving the germination rate of aged peanut seeds comprises the following steps:
s1, preprocessing: spreading the aged peanut seeds in the sun, airing for 2-3 hours, turning the seeds for 1-2 times in the middle, and irradiating each peanut seed with the sun as much as possible;
s2, preparing seed soaking liquid: adding the following components into a common aqueous solution according to the weight ratio: gibberellin GA3100-300mg/L of organic solvent, 0.1-0.3mg/L of brassinolide, 0.1-0.3mg/L of ethylene and 6-BA 0 of cytokinin1-0.3mg/L and 0.2-0.4mg/L of auxin IAA, and mixing uniformly for later use;
s3, seed soaking: soaking the aired aged peanut seeds in warm water at 50-55 ℃ for half an hour, then fishing out the seeds, then putting the seeds into a seed soaking liquid, soaking for 1-2 hours at normal temperature, and then taking out and sowing.
Preferably, the mass concentration of each substance in the step S2 is gibberellin GA3200mg/L, brassinolide 0.2mg/L, ethylene 0.1mg/L, cytokinin 6-BA 0.1mg/L and auxin IAA0.2 mg/L.
To understand the germination process of aged seeds, first to understand the principle of seed aging, which is a process that occurs gradually and involves numerous complex physiological and biochemical reactions. This is reflected in the physiological and biochemical reactions accompanying membrane lipid peroxidation, inactivation of protective enzymes, protein degradation, related gene expression disorder, nucleic acid degradation and the like. The physiological characteristics of the aged seeds of herbs and woody plants, whether artificially aged or naturally aged, show consistency: before the aged seeds die, a series of physiological and biochemical reactions occur in the aged seeds, such as changes in protective enzyme activity, endogenous hormone fluctuation, degradation of nucleic acid and protein, irreversible damage to plasma membrane structures and the like. Based on the physiological characteristic that the content of part of endogenous hormones is reduced in the process of seed aging, the reduced endogenous hormones are compensated by adding exogenous hormones to aged seeds, so that the hormone level of the aged seeds is recovered and even exceeds the level of fresh peanut seeds, and the germination rate and the germination vigor of the aged seeds are increased.
The invention adopts the principle of gibberellin, brassinolide, auxin, ethylene and cytokinin treatment: gibberellins, abscisic acid, ethylene, cytokinins, auxins, and brassinolides are six major hormones recognized throughout the world in plants. The abscisic acid has the functions of promoting seed dormancy and inhibiting seed germination, and the gibberellin has the functions of breaking dormancy and promoting germination. Ethylene, cytoclastin, brassinolide and auxin are other four important hormones in the growth and development process of plants and may participate in the regulation and control of seed germination. The method can improve the germination rate of aged peanut seeds to be close to that of fresh seeds, is a new breakthrough in the field of peanut planting, can reduce the planting cost and increase the germination success rate.
Detailed Description
In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the following examples.
Example 1
The seed soaking liquid for improving the germination rate of aged peanut seeds comprises the following components in percentage by weight:
100-300mg/L gibberellin GA30.1-0.3mg/L brassinolide, 0.1-0.3mg/L ethylene, 0.1-0.3mg/L cytokinin 6-BA, 0.2-0.4mg/L auxin IAA and the balance of water.
Example 2
A method for improving the germination rate of aged peanut seeds comprises the following steps:
s1, preprocessing: spreading the aged peanut seeds in the sun, airing for 2-3 hours, turning the seeds for 1-2 times in the middle, and irradiating each peanut seed with the sun as much as possible;
s2, preparing seed soaking liquid: adding the following components into a common aqueous solution according to the weight ratio: gibberellin GA3100 mg/L of 300-one, 0.1-0.3mg/L of brassinolide, 0.1-0.3mg/L of ethylene, 0.1-0.3mg/L of cytokinin 6-BA and 0.2-0.4mg/L of auxin IAA, and uniformly mixing for later use;
s3, seed soaking: soaking the aired aged peanut seeds in warm water at 50-55 ℃ for half an hour, then fishing out the seeds, then putting the seeds into a seed soaking liquid, soaking for 1-2 hours at normal temperature, and then taking out and sowing.
Example 3
Selecting 1800 fresh peanut seeds and 1800 aged peanut seeds which are placed at normal temperature for one year, airing for 3 hours under the sun, dividing the peanut seeds into 100 parts, immersing the 100 parts into warm water with the volume 5 times of that of the peanut seeds at 50-55 ℃ for half an hour, fishing out the peanut seeds, and soaking the seeds by adopting hormone treatment methods (shown in table 1) with different concentrations, wherein the results show that the germination potential and the germination rate of the aged peanut seeds which are not treated by the hormone are respectively only 34% and 47%; gibberellin, brassinolide and the like are independently adopted,The germination vigor and the germination rate of aged peanut seeds treated by auxin or biotin are slightly improved; the germination vigor and the germination rate of the peanut seeds treated by gibberellin, brassinolide, auxin and cytokinin are obviously improved, wherein 200mg/L gibberellin GA is adopted3The germination vigor and the germination rate of peanut seeds treated by +0.1mg/L of ethylene, 0.2mg/L of brassinolide, 0.2mg/L of auxin IAA and 0.1mg/L of cytokinin 6-BA are respectively the highest and reach 94 percent and 96 percent respectively.
In the research, the fresh peanut seeds are not treated by hormone, and the germination vigor and the germination rate are 93 percent and 95 percent respectively; after the treatment by the method, the germination potential is increased to 96-99% and the germination rate is increased to 99-100%.
TABLE 1 germination vigor and germination rate of aged peanut seeds treated with different hormone concentrations
As can be seen from Table 1, compared with the method for soaking peanut seeds in warm soup and the method for treating peanut seeds with gibberellin solution alone, the method for treating aged peanut seeds in the same batch can improve the germination rate by 48-97%, the germination rate of 4 days of germination vigor can be improved to 94%, the uniformity of emergence is obviously improved, the size of the peanut seeds is uniform, and the peanut seeds are high in vigor.
The above examples only represent some embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (4)
1. The seed soaking liquid for improving the germination rate of aged peanut seeds is characterized by comprising the following components in percentage by weight:
100-300mg/L gibberellin GA30.1-0.3mg/L brassinolide, 0.1-0.3mg/L ethylene, 0.1-0.3mg/L cytokinin 6-BA, 0.2-0.4mg/L auxin IAA and the balance of water.
2. The seed soak solution of claim 1, wherein the seed soak solution is comprised of the following components in weight concentration: 200mg/L gibberellin GA30.2mg/L brassinolide, 0.1mg/L ethylene, 0.1mg/L cytokinin 6-BA, 0.2mg/L auxin IAA and the balance of water.
3. A method for improving the germination rate of aged peanut seeds is characterized by comprising the following steps:
s1, preprocessing: spreading the aged peanut seeds in the sun, airing for 2-3 hours, turning the seeds for 1-2 times in the middle, and irradiating each peanut seed with the sun as much as possible;
s2, preparing seed soaking liquid: adding the following components into a common aqueous solution according to the weight ratio: gibberellin GA3100 mg/L of 300-one, 0.1-0.3mg/L of brassinolide, 0.1-0.3mg/L of ethylene, 0.1-0.3mg/L of cytokinin 6-BA and 0.2-0.4mg/L of auxin IAA, and uniformly mixing for later use;
s3, seed soaking: soaking the aired aged peanut seeds in warm water at 50-55 ℃ for half an hour, then fishing out the seeds, then putting the seeds into a seed soaking liquid, soaking for 1-2 hours at normal temperature, and then taking out and sowing.
4. The method of claim 3, wherein the mass concentration of each substance in the step S2 is gibberellin GA3200mg/L, brassinolide 0.2mg/L, ethylene 0.1mg/L, cytokinin 6-BA 0.1mg/L and auxin IAA0.2 mg/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109104932B (en) * | 2018-08-11 | 2020-12-18 | 山东省潍坊市农业科学院 | Pretreatment-before-sowing method for improving activity of aged asparagus seeds |
| CN109197883A (en) * | 2018-10-24 | 2019-01-15 | 华南农业大学 | A kind for the treatment of fluid and processing method improving deterioration green soy bean percentage of seedgermination |
| CN109717065B (en) * | 2019-03-20 | 2021-11-02 | 中国农业科学院农业资源与农业区划研究所 | Method for promoting germination of alfalfa |
| CN111742923A (en) * | 2020-07-14 | 2020-10-09 | 河南省林业科学研究院 | Regulator and method for breaking summer dormancy of chionanthus retusus |
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| CN103159539A (en) * | 2011-12-12 | 2013-06-19 | 蒋永直 | Potash fertilizer comprising growth regulator |
| CN104255752A (en) * | 2014-09-26 | 2015-01-07 | 山东省花生研究所 | Peanut seed coating additive |
| CN104969690A (en) * | 2015-06-12 | 2015-10-14 | 安徽徽大农业有限公司 | Peanut seed germination method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159539A (en) * | 2011-12-12 | 2013-06-19 | 蒋永直 | Potash fertilizer comprising growth regulator |
| CN104255752A (en) * | 2014-09-26 | 2015-01-07 | 山东省花生研究所 | Peanut seed coating additive |
| CN104969690A (en) * | 2015-06-12 | 2015-10-14 | 安徽徽大农业有限公司 | Peanut seed germination method |
Non-Patent Citations (1)
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| 芸苔素内酯对花生幼苗生长的影响;刘伟等;《现代农药》;20050228;第4卷(第1期);第42-43页 * |
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