CN109197883A - A kind for the treatment of fluid and processing method improving deterioration green soy bean percentage of seedgermination - Google Patents
A kind for the treatment of fluid and processing method improving deterioration green soy bean percentage of seedgermination Download PDFInfo
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- CN109197883A CN109197883A CN201811242215.9A CN201811242215A CN109197883A CN 109197883 A CN109197883 A CN 109197883A CN 201811242215 A CN201811242215 A CN 201811242215A CN 109197883 A CN109197883 A CN 109197883A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind for the treatment of fluids and processing method for improving deterioration green soy bean percentage of seedgermination, belong to agriculture field.Contain following component gibberellin GA in the processing3, ethylene, study on brassinolide, auxin IAA and basic element of cell division 6-BA.With the deterioration green soy bean seed of batch, germination percentage can be improved using treatment fluid processing of the invention to 48%-97%, 4 days emergence rates of germinating energy can be improved to 93%, and green soy bean seed sprouting uniformity significantly improves, and seedling size is uniform, energetic.In addition operation of the present invention is simple, without being coated in advance to seed, does not have to low-temperature storage.
Description
Technical field
The invention belongs to agriculture fields, and in particular to it is a kind of improve deterioration green soy bean percentage of seedgermination treatment fluid and processing side
Method.
Background technique
Green soy bean is a kind of Fresh edible soybean of fresh food, and as high protein crop, seed is easier to deterioration.Green soy bean seed is being formed
After process and maturation, in undesirable natural environment, seed vigor is lost quickly, and germination percentage reduces, referred to as deterioration.Green soy bean
Seed deterioration influences seed quality, also seriously restricts the extension of green soy bean production and planting area.Especially southern more hot humids
Weather, the seed quality reached maturity under the conditions of such is inferior, and it is improper slightly to save in addition, just has and all loses percentage of seedgermination
Possibility.Therefore, green soy bean percentage of seedgermination is improved, accelerates seed and sprouts, promotes seed sprouting to become and guarantees green soy bean stable high yield
Key link.
It is currently less to the research for improving green soy bean percentage of seedgermination, Chinese patent " the sub- heat of application number 2004100665815
Production of hybrid seeds store method with regional spring-sown type " is disclosed by using the fresh pod production of hybrid seeds of live pole harvest in advance, will under the conditions of cool place
Threshing precipitation is blowed after seed packing spreading for cooling shade is dry, then using by chitin, Dipel, natto resin, micro
Highly effective and safes such as element, guar gum and with the big of the effective component 25% that is formulated of biological agent for influencing physiological metabolism
Beans protect bud coating agent, will store under cryogenic conditions after the special coating of dry seeds, so that the pod of soybean mature and seed is avoided high temperature more
The natural conditions of rain have the effect of preventing pest and keep high-incidence bud rate.However, this method needs the fresh pod system of harvest in advance
Kind, and coating and low-temperature storage after seed dries, it is troublesome in poeration.In addition, there is also following disadvantages: seed is once coated,
If being not used in sowing, it will be unable to eat, secondly general peasant household does not have the condition of low temperature storage, it is difficult to effectively store green soy bean
Capsuled seed.In addition, the purpose of this technology is only to provide the store method of green soy bean, the bad leaf rate of green soy bean seed is reduced.But simultaneously
It is not suitable for improving the germination percentage of the green soy bean seed (including deterioration and non-deterioration) of peasant household's preservation under existence conditions.
In addition, green soy bean is rich in grease and protein, due to being rich in excessively high grease and protein, green soy bean seed belongs to short-lived
Seed, with the extension of storage time, deterioration easily occurs for seed, and seed vitality is caused to decline, and germination percentage and germinating energy reduce, from
And green soy bean seed utility value and Seed practical value is caused to reduce.Soya seeds germination nutrient solution known today etc. is for just
Normal soya seeds, without for the green soy bean seed for being deterioration.Such as Chinese patent CN107459387A, CN108391481A and
These methods of CN108377690A are not suitable for deterioration green soy bean seed.
Therefore, a kind of simple and efficient processing method for improving green soy bean percentage of seedgermination how is developed, there is important reality
Meaning.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of raising green soy bean seed (including deterioration and non-
The green soy bean seed of deterioration) germination percentage treatment fluid and processing method, it can be remarkably reinforced the vigor of deterioration green soy bean seed, improve hair
The fresh and alive quality of bud rate and sprout；In addition, can also play Dormancy breaking to fresh green soy bean seed, the effect of germination percentage is improved.
The technical solution used in the present invention is:
A kind for the treatment of fluid improving green soy bean percentage of seedgermination, the treatment fluid contain following component: 100-300mg/L's is red
Mycin GA3, the ethylene of 0.1-0.3mg/L, the study on brassinolide of 0.1-0.4mg/L, 0.2-0.5mg/L auxin IAA and
The 0.1-0.3mg/L basic element of cell division.
Preferably, the treatment fluid contains following component: the gibberellin GA of 100-300mg/L3, 0.1-0.2mg/L second
Alkene, the study on brassinolide of 0.1-0.3mg/L, 0.2-0.4mg/L auxin IAA and the 0.1-0.3mg/L basic element of cell division.
Preferably, the treatment fluid contains following component: the gibberellin GA of 100-200mg/L3, 0.1-0.2mg/L second
Alkene, the study on brassinolide of 0.1-0.3mg/L, 0.2-0.4mg/L auxin IAA and the 0.1-0.2mg/L basic element of cell division.
Preferably, the basic element of cell division includes kinetin, zeatin, 6- benzylaminopurine.
Preferably, basic element of cell division 6-BA.
In general, the basic element of cell division includes kinetin (KT), zeatin (ZT), 6- benzylaminopurine (6-BA) etc..
Since 6-BA is quality-high and inexpensive, and effect is good, therefore the present invention uses 6-BA.
Preferably, the treatment fluid also contains Adenosine Triphosphate Disodium salt ATP, and content is preferably 10-25mg/L.
Gradually occur since seed deterioration is one and be related to the process of numerous complicated biochemical reactions.This is embodied in
Along with Lipid peroxidation metabolism, enzymatic inactivation, protein degradation, the Physiology and biochemistries such as related gene expression disorder and nucleolysis are protected
Reaction.Whether the physiological property of Artificial deterioration or natural deterioration, draft and Woody plant seed deterioration is deposited in various degree
In following characteristics: deterioration seed before death, inside a series of biochemical reactions will occur, such as defence enzyme activity
Change, the fluctuation of endogenous hormones, the degradation of nucleic acid and protein, plasma structure is irreversibly damaged etc..
Applicants have found that Endogenous Hormone Contents in Vitro is reduced after green soy bean seed deterioration, ATP content is decreased obviously, so logical
It crosses research and development and filters out gibberellin, ethylene, study on brassinolide, auxin and the basic element of cell division 5 big growth hormone and ATP progress in total
Compounding.Reduced endogenous hormones are made up by adding exogenous hormone to deterioration green soy bean seed, make the hormone of deterioration green soy bean seed
Level restores the level for being even more than fresh green soy bean seed, to increase the germination percentage and germinating energy of deterioration green soy bean seed.May be used also
Adenosine Triphosphate Disodium salt ATP is added or not added with selection as needed.
Since growth hormone has the function of bidirectional modulation.As gibberellin GA3It is more than 0.2mg/ more than 300mg/L, ethylene
L, it be more than 0.4mg/L and basic element of cell division 6-BA is more than that 0.3mg/L can inhibit that study on brassinolide, which is more than 0.3mg/L, auxin IAA,
The germination percentage and germinating energy of deterioration green soy bean seed.
It is furthermore preferred that the content of Adenosine Triphosphate Disodium salt ATP is 10-20mg/L.Can according to need selection addition or
Adenosine Triphosphate Disodium salt ATP is not added.
Preferably, the green soy bean seed includes fresh green soy bean seed and the green soy bean seed by storage, by the hair of storage
Beans seed includes deterioration green soy bean seed, non-deterioration green soy bean seed.
A kind of processing method improving green soy bean percentage of seedgermination is using treatment fluid described in any of the above embodiments to green soy bean kind
Son carries out immersion treatment, is then sowed again.
Preferably, the temperature when treatment fluid carries out immersion treatment to green soy bean seed is room temperature.
Preferably, green soy bean seed is first impregnated with 45-50 DEG C of water of 4-6 times of volume before the immersion treatment for carrying out treatment fluid
20-40min carries out vernalization processing.
Preferably, it before 45-50 DEG C of water of 4-6 times of volume of green soy bean seed impregnates 20-40min, need to be carried out under sunlight
2.5-3.5h is dried, to improve the germination percentage and germinating energy of green soy bean seed.
The beneficial effects of the present invention are:
With the deterioration green soy bean seed of batch, germination percentage can be improved to 48%-97% using treatment fluid processing of the invention,
4 days emergence rates of germinating energy can be improved to 93%, and green soy bean seed sprouting uniformity significantly improves, and seedling size is uniform, energetic.In addition
Operation of the present invention is simple, without being coated in advance to seed, does not have to low-temperature storage.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is described in detail, but not limited to this.
Embodiment 1
Select fresh green soy bean seed (in order to allow data to have comparativity, fresh green soy bean also carries out same treatment) and room temperature decentralization
Each 1900, deterioration green soy bean seed of 1 year are set, sunning 3 hours is carried out first under sunlight, is then divided into green soy bean seed
100 portions, and half an hour in 45-50 DEG C of warm water of 5 times of volumes of immersion, green soy bean seed is pulled out, using swashing for various concentration
Plain processing method (table 1) seed soaking, the results are shown in Table 1.
1 hormon concentration of table treated deterioration green soy bean potentiality of seed and germination percentage
Table 1 the result shows that, not using HORMONE TREATMENT deterioration green soy bean potentiality of seed and germination percentage it is minimum, only have respectively
32% and 45%；Individually use gibberellin GA3, ethylene, study on brassinolide, auxin IAA or the basic element of cell division 6-BA processing
Deterioration green soy bean potentiality of seed and germination percentage slightly improve；Using gibberellin GA3+ ethylene+study on brassinolide+auxin IAA+ is thin
The green soy bean potentiality of seed and germination percentage of born of the same parents' mitogen 6-BA processing significantly improve, wherein red mould using 200mg/L in group 15
Plain GA3At+0.1mg/L ethylene+0.2mg/L study on brassinolide+0.2mg/L auxin IAA+0.1mg/L basic element of cell division 6-BA
The equal highest of deterioration green soy bean potentiality of seed and germination percentage of reason, respectively reaches 93% and 95%, and the germination of fresh green soy bean seed
Gesture and germination percentage are respectively 96% and 100%.But excessive application hormone can also inhibit deterioration green soy bean potentiality of seed and germination percentage,
300mg/L gibberellin GA is wherein used in group 193+ 0.3mg/L ethylene+0.4mg/L study on brassinolide+0.5mg/L auxin
The green soy bean potentiality of seed and germination percentage of the deterioration of IAA+0.3mg/L basic element of cell division 6-BA processing, averagely only have 34% respectively
With 48%, and the germinating energy of fresh green soy bean seed and germination percentage distinguish average out to 93% and 94%.
Embodiment 2
Select fresh green soy bean seed (in order to allow data to have comparativity, fresh green soy bean also carries out same treatment) and room temperature decentralization
Each 3600, deterioration green soy bean seed of 1 year are set, sunning 3 hours is carried out first under sunlight, is then divided into green soy bean seed
100 portions, and half an hour in 45-50 DEG C of warm water of 5 times of volumes of immersion, green soy bean seed are pulled out, using the medicine of various concentration
Agent processing method (table 2) seed soaking, the results are shown in Table 2.
Deterioration green soy bean potentiality of seed and germination percentage after 2 different disposal of table
Table 2 the result shows that, not using HORMONE TREATMENT deterioration green soy bean potentiality of seed and germination percentage it is minimum, only have respectively
33% and 46%；Individually use Adenosine Triphosphate Disodium salt, gibberellin GA3, study on brassinolide, auxin IAA, ethylene or cell
The deterioration green soy bean potentiality of seed and germination percentage of mitogen 6-BA processing slightly improve, wherein with application 15mg/L adenosine triphosphate
Disodium salt is compared, and excessive apply Adenosine Triphosphate Disodium salt (25mg/L) does not have shadow to deterioration green soy bean potentiality of seed and germination percentage
It rings；Using Adenosine Triphosphate Disodium salt, gibberellin GA3, study on brassinolide, auxin IAA, ethylene or basic element of cell division 6-BA two
Two compoundings, deterioration green soy bean potentiality of seed and germination percentage increase, but also fail to reach preferable effect.Using adenosine three
Disodic alkaliine+gibberellin GA3+ ethylene+study on brassinolide+auxin IAA+ basic element of cell division 6-BA processing deterioration green soy bean kind
Sub- germinating energy and germination percentage significantly improve, and 15mg/L Adenosine Triphosphate Disodium salt+200mg/L gibberellin is wherein used in group 33
GA3+ 0.1mg/L ethylene+0.2mg/L study on brassinolide+0.2mg/L auxin IAA+0.1mg/L basic element of cell division 6-BA processing
Deterioration green soy bean potentiality of seed and the equal highest of germination percentage, respectively reach 94% and 96%.And fresh green soy bean seed it is untreated when,
Its germinating energy and germination percentage are respectively 92% and 94%；After being handled using this method, germinating energy increases to 97% and germination percentage increasing
Add to 100%.
Claims (9)
1. a kind for the treatment of fluid for improving green soy bean percentage of seedgermination, it is characterised in that: the treatment fluid contains following component: 100-
The gibberellin GA of 300mg/L3, the ethylene of 0.1-0.3mg/L, the study on brassinolide of 0.1-0.4mg/L, 0.2-0.5mg/L life
The long element IAA and 0.1-0.3mg/L basic element of cell division.
2. treatment fluid according to claim 1, it is characterised in that: the treatment fluid contains following component: 100-300mg/L
Gibberellin GA3, the ethylene of 0.1-0.2mg/L, the study on brassinolide of 0.1-0.3mg/L, 0.2-0.4mg/L auxin IAA
With the 0.1-0.3mg/L basic element of cell division.
3. treatment fluid according to claim 2, it is characterised in that: the basic element of cell division includes kinetin, zeatin, 6- benzyl
Adenine phosphate.
4. treatment fluid according to claim 1-3, it is characterised in that: the treatment fluid also contains adenosine triphosphate
Disodium salt ATP, content are preferably 10-25mg/L.
5. treatment fluid according to claim 1-3, it is characterised in that: the green soy bean seed includes fresh green soy bean kind
Son and the green soy bean seed by storing, the green soy bean seed by storage includes deterioration green soy bean seed, non-deterioration green soy bean seed.
6. a kind of processing method for improving green soy bean percentage of seedgermination, it is characterised in that: be using described in any one of claim 1-5
Treatment fluid to green soy bean seed carry out immersion treatment, then sowed again.
7. processing method according to claim 6, it is characterised in that: the treatment fluid carries out immersion treatment to green soy bean seed
When temperature be room temperature.
8. processing method according to claim 7, it is characterised in that: green soy bean seed is in the immersion treatment for carrying out treatment fluid
Before, first 20-40min is impregnated with 45-50 DEG C of water.
9. processing method according to claim 8, it is characterised in that: green soy bean seed impregnates 20-40min with 45-50 DEG C of water
Before, sunning 2.5-3.5h need to be carried out under sunlight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811242215.9A CN109197883A (en) | 2018-10-24 | 2018-10-24 | A kind for the treatment of fluid and processing method improving deterioration green soy bean percentage of seedgermination |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811242215.9A CN109197883A (en) | 2018-10-24 | 2018-10-24 | A kind for the treatment of fluid and processing method improving deterioration green soy bean percentage of seedgermination |
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| CN109197883A true CN109197883A (en) | 2019-01-15 |
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ID=64996351
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| CN201811242215.9A Withdrawn CN109197883A (en) | 2018-10-24 | 2018-10-24 | A kind for the treatment of fluid and processing method improving deterioration green soy bean percentage of seedgermination |
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| CN1915032A (en) * | 2006-09-12 | 2007-02-21 | 中国农业大学 | Seed treatment in use for accelerating germination of seed |
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| CN103951523A (en) * | 2014-05-18 | 2014-07-30 | 重庆市优胜科技发展有限公司 | Balance growth regulator for plant and preparation method of balance growth regulator |
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| CN107787636A (en) * | 2017-09-28 | 2018-03-13 | 四川农业大学 | A kind of method for promoting aging Germination of Soybean Seed |
| CN107751224A (en) * | 2017-11-22 | 2018-03-06 | 广东省农业科学院作物研究所 | A kind of seed-soaking liquid and method for improving aging peanut seed germination percentage |
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Application publication date: 20190115 |
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