CN107787636A - A kind of method for promoting aging Germination of Soybean Seed - Google Patents
A kind of method for promoting aging Germination of Soybean Seed Download PDFInfo
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- CN107787636A CN107787636A CN201710896956.8A CN201710896956A CN107787636A CN 107787636 A CN107787636 A CN 107787636A CN 201710896956 A CN201710896956 A CN 201710896956A CN 107787636 A CN107787636 A CN 107787636A
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- soybean
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- 244000068988 Glycine max Species 0.000 title claims abstract description 94
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 92
- 230000032683 aging Effects 0.000 title claims abstract description 76
- 238000000034 method Methods 0.000 title claims abstract description 57
- 230000035784 germination Effects 0.000 title claims abstract description 36
- 230000001737 promoting effect Effects 0.000 title claims abstract description 16
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- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- -1 Ethanol ester Chemical class 0.000 description 2
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- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
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- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical group OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of method for promoting aging Germination of Soybean Seed, comprise the following steps：Selection size is homogeneous after choosing natural air drying, full grains, the soya seeds of the aging of no disease and pests harm, returns dry 2d at room temperature, standby；The seed chosen is put into the clean culture dish for being covered with double-layer filter paper；Under constant temperature non-illuminated conditions, diethylamino ethanol caproate solution is added in culture dish, seed is sprouted wherein；The soya seeds sprouted are planted in soil, photoperiod same natural environment, during emergence, spray appropriate running water at regular time and quantity.DA 6 of the present invention is photosynthetic to aging Germination of Soybean Seed and subsequent seedling to be built up with obvious facilitation；Sprouted for seed and more multi-energy is provided, and add the chlorophyll content after seedling establishment, promoted the carbon assimilation ability of plant, finally ensure that the sprouting of Aged Seeds and the photosynthetic of seedling build up.
Description
Technical field
The invention belongs to soybean to cultivate field, specifically, is related to a kind of method for promoting aging Germination of Soybean Seed.
Background technology
Soybean (Glycine max) has long cultivation in China as the grain rich in protein and oil crops
History.20th century, China was one of Soybean production important in the world and exported country, with the drastically reduction of cultivated area, was needed
Ask and be constantly increasing, nowadays China soybean is changed into import by exporting, the serious grain security that threaten China.Therefore,
The Soybean Industry for greatly developing the country is urgent problem to be solved during current Soybean production.But the work of soya seeds
Power is protected from environmental larger, and aging easily occurs during storage, and seed vitality drastically reduces, in agricultural production
To sprouting large effect is caused with emergence.The serious development for constraining China's Soybean Industry of the aging of soya seeds, causes
Extensive concern.
After maturation inevitably all aging is occurring for seed, and seed coat structure damages, the energy of internal reservoir
Peroxidization, damage of genetic materials etc., which occurs, for material causes seed longeivity to shorten.Soya seeds are during storage by ring
Border has a great influence, and aging easily occurs during storage is harvested.The preservation of Soybean Germplasm governs agricultural production
In industrialization and mechanization, with the development of mechanized agriculture, stricter is required to the vigor of crop seed, this causes
Improve Germination of Soybean Seed rate and seem particularly significant.
The content of the invention
In view of this, the present invention is directed to the problem of above-mentioned, there is provided a kind of method for promoting aging Germination of Soybean Seed.
In order to solve the above-mentioned technical problem, the invention discloses a kind of method for promoting aging Germination of Soybean Seed, including
Following steps：
Step 1, Seeds preprocess：Selection size is homogeneous after choosing natural air drying, full grains, the aging of no disease and pests harm
Soya seeds, return dry 2d at room temperature, it is standby；
The seed that step 2, selecting step 1 obtain, puts it into the clean culture dish for being covered with double-layer filter paper；
Step 3, under constant temperature non-illuminated conditions, in culture dish add diethylamino ethanol caproate solution, make seed
Sprout wherein；
Step 4, the soya seeds for handling and having sprouted by step 3 are planted in soil, the photoperiod is the same as natural
Environment, during emergence, appropriate running water is sprayed at regular time and quantity.
Further, the concentration of diethylamino ethanol caproate solution is 100-400 μM in step 3.
Further, the sprout time of seed is 12-24h in step 3.
Further, environment temperature is 20-25 DEG C in step 4.
Compared with prior art, the present invention can be obtained including following technique effect：
DA-6 of the present invention is photosynthetic to aging Germination of Soybean Seed and subsequent seedling to be built up with obvious facilitation.
Its mechanism is, by promoting conversion of the grease to sugar, more multi-energy is provided for seed sprouting, and after adding seedling establishment
Chlorophyll content, promote the carbon assimilation ability of plant, finally ensure that the sprouting of Aged Seeds and the photosynthetic of seedling are built
Into.
Certainly, any product for implementing the present invention it is not absolutely required to reach all the above technique effect simultaneously.
Brief description of the drawings
Accompanying drawing described herein is used for providing a further understanding of the present invention, forms the part of the present invention, this hair
Bright schematic description and description is used to explain the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 is that the present invention applies DA- by burin-in process and without the soya seeds of burin-in process in germination process
The influence of 6 pairs of its germination rates；Wherein, phenotype picture when A, B and C are 60h in Seed Germination；D is soya seeds in difference
Treatment conditions under sprouting situation statistical chart；A be it is unaged be that normal soya seeds are sprouted under the conditions of clear water；B：Aging+
Water is being sprouted under the conditions of 0 μM with 200 μM of DA-6 after burin-in process；C：Aging+DA-6 be burin-in process after at 0 μM
With being sprouted under the conditions of 200 μM of DA-6；
Fig. 2 is normal soya seeds sprouting situation under the conditions of the DA-6 of various concentrations of the invention；Wherein, CK is just
Normal soya seeds are sprouted in clear water；200 μM are that normal soya seeds are sprouted in 200 μM of DA-6；
Fig. 3 be the present invention by aging with without Germination of Soybean Seed 70h after radicle length and its fresh weight, A is
Radicle length after Germination of Soybean Seed；B is the fresh weight of radicle；C is the dry weight of radicle；It is unaged to exist for normal soya seeds
Sprouted under the conditions of clear water；Aging+water and aging+DA-6 are being sprouted under the conditions of 0 μM with 200 μM of DA-6 after burin-in process
Hair；
Fig. 4 is the present invention by aging and untreated soya seeds seedling establishment ability；Wherein, A is to have sprouted
The soya seeds of hair are transplanted to after soil seedling establishment rate after 20d；B is 20d seedling establishments statistics after soya seeds transplanting；C is
Plant height phenotypic map after seedling establishment；D be seedling establishment after plant height statistics, Bar=5cm；Unaged is normal soya seeds
Sprouted under the conditions of clear water；Aging+water and aging+DA-6 be after burin-in process under the conditions of 0 μM with 200 μM of DA-6
Sprout；
Fig. 5 is influence of the different disposal of the present invention to character after soya seeds seedling establishment；Wherein, A is different disposal
Under the conditions of, soybean seedling builds up the difference of rear dry weight；B is chlorophyll content after the seed seedling establishment sprouted under different disposal
Difference；It is unaged to be sprouted for normal soya seeds under the conditions of clear water；After aging+water and aging+DA-6 are burin-in process
Sprouted under the conditions of 0 μM with 200 μM of DA-6；
Fig. 6 is the change of seed imbibition different time sugared content of the present invention；A seeds different disposal condition during imbibition
The change of lower cane sugar content；During B seed imbibitions under the conditions of different disposal fructose content change；It is different during C seed imbibitions
The change of the lower content of reducing sugar of processing；During D seed imbibitions under different disposal Soluble adhesion molecule change；It is wherein unaged
Sprouted for normal soya seeds under the conditions of clear water；Aging+water and aging+DA-6 are at 0 μM and 200 after burin-in process
μM DA-6 under the conditions of sprout；Three block diagrams at each moment be followed successively by from left to right unaged, aging+water and aging+
DA-6；
Fig. 7 is the vigour changes of seed of the present invention invertase during imbibition；Soya seeds wherein after aging
The more not aged soya seeds of most of its invertase are low, and being sprouted wherein after aging in clear water and in DA-6 has pole
Significant difference, wherein unaged sprout for normal soya seeds under the conditions of clear water；Aging+water and aging+DA-6 are old
Being sprouted under the conditions of 0 μM with 200 μM of DA-6 after change processing；Three block diagrams at each moment are followed successively by not from left to right
Aging, aging+water and aging+DA-6；
Fig. 8 be seed of the present invention again during imbibition content of fatty acid change；Wherein B is that GC-MS detects Soybean Species
The total amount of all aliphatic acid in son, C are the total amount of the unrighted acid detected；D is the total of the saturated fatty acid that detects
Amount；
Fig. 9 is the expression quantity of some genes during seed imbibition in the present invention, and wherein A-D is in Seed Germination,
From some key genes of the decomposition of aliphatic acid into glucide conversion process；E is the LOX in Seed Germination
Expression quantity detection.
Embodiment
Describe embodiments of the present invention in detail below in conjunction with embodiment, thereby to the present invention how application technology
Means can fully understand and implement according to this to solve technical problem and reach the implementation process of technical effect.
The invention discloses a kind of method for promoting aging Germination of Soybean Seed, comprise the following steps：
Step 1, Seeds preprocess：Selection size is homogeneous after choosing natural air drying, full grains, the aging of no disease and pests harm
Soya seeds (the storage time limit more than 1 year and plant skin do not completely lose sprouting ability without going mouldy), Hui Gan at room temperature
2d, it is standby；
The seed that step 2, selecting step 1 obtain, puts it into the clean culture dish for being covered with double-layer filter paper；
Step 3, under constant temperature non-illuminated conditions, it is 100-400 μM of caproic acid lignocaine that concentration is added in culture dish
Ethanol ester (DA-6) solution, makes seed sprout 12-24h wherein；
Step 4, the soya seeds for handling and having sprouted by step 3 are planted in soil, environment temperature is
20-25 DEG C, photoperiod same natural environment, during emergence, appropriate running water is sprayed at regular time and quantity.
Embodiment 1
A kind of method for promoting aging Germination of Soybean Seed, comprises the following steps：
Step 1, Seeds preprocess：Selection size is homogeneous after choosing natural air drying, full grains, the aging of no disease and pests harm
Soya seeds, return dry 2d at room temperature, it is standby；
The seed that step 2, selecting step 1 obtain, puts it into the clean culture dish for being covered with double-layer filter paper；
Step 3, under constant temperature non-illuminated conditions, it is 250 μM of hexanoic acid diethylaminoethanol that concentration is added in culture dish
Ester (DA-6) solution, makes seed sprout 18h wherein；
Step 4, the soya seeds for handling and having sprouted by step 3 are planted in soil, environment temperature 22
DEG C, photoperiod same natural environment, during emergence, appropriate running water is sprayed at regular time and quantity.
Embodiment 2
A kind of method for promoting aging Germination of Soybean Seed, comprises the following steps：
Step 1, Seeds preprocess：Selection size is homogeneous after choosing natural air drying, full grains, the aging of no disease and pests harm
Soya seeds, return dry 2d at room temperature, it is standby；
The seed that step 2, selecting step 1 obtain, puts it into the clean culture dish for being covered with double-layer filter paper；
Step 3, under constant temperature non-illuminated conditions, it is 100 μM of hexanoic acid diethylaminoethanol that concentration is added in culture dish
Ester (DA-6) solution, makes seed sprout 24h wherein；
Step 4, the soya seeds for handling and having sprouted by step 3 are planted in soil, environment temperature 20
DEG C, photoperiod same natural environment, during emergence, appropriate running water is sprayed at regular time and quantity.
Embodiment 3
A kind of method for promoting aging Germination of Soybean Seed, comprises the following steps：
Step 1, Seeds preprocess：Selection size is homogeneous after choosing natural air drying, full grains, the aging of no disease and pests harm
Soya seeds, return dry 2d at room temperature, it is standby；
The seed that step 2, selecting step 1 obtain, puts it into the clean culture dish for being covered with double-layer filter paper；
Step 3, under constant temperature non-illuminated conditions, it is 400 μM of hexanoic acid diethylaminoethanol that concentration is added in culture dish
Ester (DA-6) solution, makes seed sprout 12h wherein；
Step 4, the soya seeds for handling and having sprouted by step 3 are planted in soil, environment temperature 25
DEG C, photoperiod same natural environment, during emergence, appropriate running water is sprayed at regular time and quantity.
Illustrate the technique effect of the present invention with reference to specific experiment：
1 materials and methods
1.1 experiment material
Soybean varieties are tested as " southern beans 12 ", seed is by Sichuan Agricultural University's " Sichuan Province's crop banding composite plant engineering
Technical Research Center " provides.
1.2 experiment process
Normal soya seeds are subjected to Artificial ageing or the soya seeds from natural aging, afterwards in room
Temperature is sprouted after doing 2d next time to it.
Take diameter 9cm culture dish, spread two layers of middling speed qualitative filter paper, be separately added into by with without burin-in process
" the southern soya seeds of beans 12 " 20, it is 100,200,300,400 μM that concentration is separately added into the ware Jing Guo burin-in process
DA-6 solution 20mL, ultra-pure water, 200,400 μM of DA-6 solution are separately added into the culture dish without burin-in process
20mL, each 3 experiments of Setup Experiments repeat and biology repeats, and are placed on 25 DEG C of insulating box (models：Sanyo
Versatile Environmental Test Chamber MLR-350H) it is inner it is dark sprout 2 days, united during this period per 12h
Germination rate is counted, to determine its phenotype.It is time point determining sugared content in 0h, 12h, 24h, 36h, 48h.
1.3 testing indexs and method
1.3.1 artificial accelerated aging processing accelerates artificial ageing method or selection using (58 ± 1 DEG C) hot water in this experiment
The soya seeds of natural aging, seed is placed in the water bath with thermostatic control of (58 ± 1 DEG C) natural air drying after processing 20min, treats seed
Do to carrying out sprouting experiment during original state.
1.3.2 germination rate statistics seed is sprouted in constant incubator under dark condition, using the lamp of green as light
Source, count and sprout in different time points, the influence that illumination is sprouted to seed is preferably minimized, 2mm is more than with seed germination length
Criterion as sprouting.Radicle length is measured using Image J softwares, weight electronic balance weighing.
1.3.3 the seed sprouted and growing way is consistent is seeded into soil by seedling establishment statistics, in formed objects
Sowing quantity is identical in basin, counts and counts its normal seedling numbers that are unearthed, and growing in after planting 7d.Determined in 20d
Plant height；Plant is removed from soil, blotted after rinsing rhizosphere soil well with clear water with blotting paper, electronic balance weighing is fresh
Weight.Seedling by above-mentioned processing finishes 15min under the conditions of 105 DEG C, and rear temperature adjustment is dried to after constant weight to 75 DEG C claims it dry
Weight, claim the biomass of individual tree of whole plant respectively.
1.3.4 measuring chlorophyll content chooses each 3 plants of seedling under each processing, takes the identical leaf position of above-ground plant parts complete
The blade deployed entirely, (unfold, Zhang Xiaosu, Chen Juan with reference to unfolding, wait simplification [J] plant physiology of measuring chlorophyll contents
Communication, 2010, (04):Method 399-402.) etc., 24h is extracted using 80% acetone, determines chlorophyll content.
1.3.4 the different sugar assay period different between Their Seed Germinating Period (0h, 12h, 24h, 36h, 48h) enters
Row sampling, loads paper bag after the moisture on surface is blotted with blotting paper.Finish 15min at 105 DEG C, after 75 DEG C are dried to constant weight,
The grind into powder in clean mortar, it is standby to load eppendorf pipes.Soluble sugar content uses anthrone colorimetry, also
Raw sugar is determined using DNS, and the content of sucrose and fructose determines (Ruan Songlin, Xue Qingzhong, Wang Qinghua seeds with Resorcinol Method
Trigger physiological effect [J] Scientia Agricultura Sinicas to hybrid rice seedling salt tolerance, 2003,36 (4):463-468.).
1.3.5 the measure of the sucrase activity period different between Their Seed Germinating Period (0h, 12h, 24h, 36h,
48h) it is sampled, -80 DEG C of preservations is put into after quick-frozen in liquid nitrogen.Assay method uses 3,5 dinitrosalicylic acids (DNS) method.
1.3.6 the measure of the content of fatty acid period different between Their Seed Germinating Period (0h, 12h, 24h, 36h, 48h)
It is sampled, -80 DEG C of preservations is put into after quick-frozen in liquid nitrogen.Using the aliphatic acid in n-hexane ultrasonic extraction soybean kernel, on
Extra large Zhen Huai biologies aliphatic acid mixing mark product, Shimadzu GC-MS 2010 analyze its composition and its content.
1.3.7 related gene expression analysis and utilization hot phenol method extraction soybean total serum IgE, 1% agarose gel electrophoresis measure
RNA integralities, Nanodrop plus measure concentration and purity, precious biological MLV enzymes carry out the synthesis of the chains of cDNA first, concrete operations
With reference to its specification.Design of primers utilizes soybean Phytozome v10.3 database analysises, and NCBI sequence analyses obtain correlation
Soybean gene primer (table 1).3 repetitions of each material, using 2-△△CT, SPSS progress significance analysis, Microsoft
Excel 2013 maps.
The related soybean gene primer of table 1
1.4 data statistic analysis
Experimental data is arranged using the softwares of Microsoft Excel 2013, and variance analysis is united using SPSS 19.0
Analysis software is counted, the comparison between average value uses one-way analysis of variance method (One-Way ANOVA)；Between different disposal
Conspicuousness use Duncan multiple comparative tests, linear measure longimetry is determined using Image J.
2 results and analysis
Influences of 2.1 DA-6 to aging Germination of Soybean Seed
Seed by aging is sprouted under different DA-6 concentration, at 200 μM it can be seen from (Fig. 1-A, B)
Under DA-6 concentration, it significantly increases effect to the Germination of Soybean Seed rate after aging.Summary result, follow-up
200 μM of the concentration to being had a great influence in Aged Seeds germination process is have chosen in experiment.
DA-6 sprouts for the seed of aging certain facilitation, when it acts on normal seed, not
It was found that there is lifting germination rate effect, simply during seed is sprouted, speed is sprouted to it slight influence, but most
Whole germination rate basically reaches consistent level (Fig. 2) with compareing.DA-6 is single-minded to improve the big of aging as can be seen here
Beans germination rate.
When by aging with without aging soya seeds sprout at different processing conditions after (70h) after profit
With its radicle length of Image J softwares subsidiaries, and claim its weight with electronic balance, as a result show, by burin-in process
After soya seeds are sprouted in DA-6, its radicle length and weight in clear water compared with sprouting (the figure that increases significantly after aging
3)。
Seedling establishment and the photosynthetic influence built up after 2.2 DA-6 sprout to Aged Seeds
Soya seeds after aging are sprouted under the conditions of DA-6, transplant the consistent seed of growing way in soil after 2d, can
To find out that the soya seeds by burin-in process are sprouted under the conditions of DA-6, its seedling establishment ability is significantly higher than in clear water
Under the conditions of the seedling establishment rate (up to 91%) sprouted, with the CK (Soybean Species sprouted without burin-in process under the conditions of clear water
Son) seedling establishment rate is quite (Fig. 4 A, B).When control, which grows into first compound leaf, to be fully deployed, measure its plant height, fresh weight,
Dry weight, the seed after aging can be seen that under the conditions of the DA-6 of 200 μM of concentration by Fig. 4 C, D, plant height obtains extremely significantly carrying
Height, substantially resume to CK levels.
To the seedling after photomorphogenesis, the analysis of further biological character is carried out, claims the dry of its individual plant respectively
Weight, it can be found that the seedling dry weight of the Seed Development after burin-in process is low compared with CK, but sprouted after aging by DA-6
The seedling dry weight less DA-6 processing of Seed Development has extremely significantly (P<0.01) raising (Fig. 5 A).
Meanwhile the seed after burin-in process is sprouted and sprouted in clear water in DA-6, the seed sprouted in DA-6 its
Chlorophyll content has significantly (P compared with CK after seedling establishment<0.05) improve, and be higher than not aged seed (Fig. 5 B).
Illustrate to increase chlorophyll content using DA-6, show that DA-6 has forward direction to the seedling establishment for improving aging soya seeds
Regulating and controlling effect, be indicated above the accumulation that DA-6 promotes biomass to a certain extent.
2.3 DA-6 promote the influence of sugared content in Aged Seeds germination process
Although the seed after burin-in process, during sprouting, sugared content, which relatively compares CK, notable or extremely significant drop
Low, but the seed after DA-6 is handled, its cane sugar content is but overall compared with the height (Fig. 6 A) handled without DA-6
Variation tendency shows the trend for first raising and reducing afterwards.
As sucrose, soluble sugar also shows first to raise the trend reduced afterwards, but in different time points, warp
The content crossed after burin-in process is always low compared with without the content of burin-in process, the seed handled in 12h, 24h by DA-6
Middle soluble sugar content is higher than the seed (Fig. 6 B) handled without DA-6.
2.4 DA-6 influence the enzymatic activity in Seed Germination
For seed during sprouting, carbohydrate is the important component of its energy supply, and cane sugar hydrolytic enzyme is in plant tissue
By its, main assimilation products sucrose is irreversible is converted into glucose and fructose, provides available hexose for plant, is
The oxidative phosphorylation of respiration coupling produces energy, and the growth with plant has close relationship, to a certain extent can be anti-
Reflect its upgrowth situation.
In this experiment, the soya seeds by burin-in process are sprouted in DA-6 and water, and its germination rate has more significant
Difference, also there occurs corresponding change for its internal sucrase activity.Data are shown, after burin-in process, are sprouted in clear water
The seed stage sucrose inversion of hair not the active seed for being markedly inferior to sprout in DA-6 after aging.Sprouted in DA-6
Its internal sucrase activity of seed reaches substantially with normally beating the sucrase activity in even Seed Germination
Suitable level (Fig. 7) is arrived.
Influences of 2.5 DA-6 to content of fatty acid in aging Soybean Seeds Germination
Soybean is as important oil crops, and its internal reservoir a large amount of undersaturated aliphatic acid, and seed is in sprouting
During, aliphatic acid is decomposed and is changed into the carbohydrate that energy can be provided for growth and development of plants, while in seed storage
During, becoming sour to a certain extent can occur for aliphatic acid, finally be changed into the peroxide or MDA of aliphatic acid
(MDA) vigor and its germination rate of seed, are influenceed.
As shown by data of the present invention, soya seeds by burin-in process are during seed is sprouted, either aliphatic acid
Totally showing aging+DA-6 is higher than aging+water (Fig. 8) for total amount, polyunsaturated fatty acid, monounsaturated fatty acids so that
The storage level of aliphatic acid is bigger, and more sufficient energy is provided for the sprouting of seed.
Influences of 2.6 DA-6 to gene expression amount related to metabolism in aging soya seeds
During seed is sprouted, the storage characteristics material of Interior Seed is decomposed metabolism, be its sprouting is provided must
The energy of palpus.During seed is sprouted, acetylcoenzyme a is to connect three major nutrient metabolic pathway, into three
Carboxylic acid recycle and oxidative phosphorylation, energy is provided for it.By the soya seeds of Artificial ageing in its germination process,
The gene expression amount for the seed regulation and control acetylcoenzyme a oxidizing ferment (ACX2) sprouted in DA-6 has significantly compared with what is sprouted in water
Ground up-regulation effect, meanwhile, related gene SDP1, SDP6 expression quantity for participating in energetic supersession has up-regulation compared with clear water.In gluconeogenesis
In approach, regulation and control catalysis oxaloacetic acid forms the expression of the gene (PCK1) of the enzyme of PEP and carbon dioxide
Amount also shows up-regulation trend.On the contrary, the seed that the expression quantity of LOX gene (LOX3) is sprouted in DA-6 after weathering
Middle performance is lowered, and the oxidation or peroxidization for making aliphatic acid reduce (Fig. 9)；I.e.：During seed is sprouted, DA- is added
6 can cause lipolysis to be raised to the expression quantity of the key gene during sugar, regulate and control the LOX3 of oxidative cleavage of fatty acids
The expression quantity of gene is lowered so that the decomposition of aliphatic acid is most of as glucide conversion, and energy is provided for the sprouting of seed.
3 discuss
Soybean has substantial amounts of aliphatic acid as oil crop seeds, its internal reservoir, accounts for more than the 90% of grease total amount.
Seed is during sprouting, direct substrate of the carbohydrate as respiration, has during seed is sprouted different degrees of
Decline.Present invention demonstrates that soya seeds, during aging, sucrose, reduced sugar, soluble sugar content relatively compare significantly (P
<0.05) reduce, by the seed of aging, germination rate obtains notable (P in the DA-6 solution containing 200 μM<0.05) carry
Height, and its cane sugar content less DA-6 processing has extremely significantly (P<0.01) raising.This is probably because DA-6 is improved
The activity of lipase, improves the fatty acid catabolism in soya seeds, accelerates conversion of the lipid to sugar, is seed
Respiration during sprouting provides the substrate of abundance, ensures the energy supply of Seed Germination.
For the soya seeds after burin-in process, seedling establishment ability significantly decreases relative to control, but
It is.The seedling establishment rate of seed after burin-in process after being sprouted in DA-6 significantly (P<0.05) it is higher than without DA-
The level of 6 processing, and its seedling dry weight built up has extremely significantly (P compared with the dry weight handled without DA-6<0.01) carry
It is high.With reference to the measurement result of chlorophyll content, DA-6 processing can significantly (P<0.05) chlorophyll content is improved.Therefore its
The increase of dry weight is probably because DA-6 by improving chlorophyll content improves crop carbon assimilation ability so that crop
Photosynthesis strengthen and then add the accumulation of dry.
The present invention has filtered out ideal DA-6 concentration, to improve aging " the sprouting energy of the southern soya seeds of beans 12 "
Power, seedling establishment and the photosynthetic ability of building up.The levels of peroxide increase of seed lipid by aging, MDA (MDA) content
Rise, lipase activity declines, gluconeogenesis reduced capability.With reference to above-mentioned data, it can be seen that DA-6 may be by enhancing fat
The property of fat enzyme, conversion of the lipid to glucide is accelerated, and then increase the content of the soluble sugars such as sucrose, be seed
Sprout and direct energy is provided.After seed is sprouted, DA-6 promotes the biosynthesis of chlorophyll, makes the chlorophyll in seedling
Content increase, and then the photosynthesis of seedling and the utilization rate of luminous energy, carbon assimilation ability may be enhanced.Cause the dry of plant
Increase again, biomass accumulation is significantly raised, is laid a good foundation for the growth in plant later stage.
Some preferred embodiments of invention have shown and described in described above, but as previously described, it should be understood that invention is not
Form disclosed herein is confined to, is not to be taken as the exclusion to other embodiment, and can be used for various other combinations, repair
Change and environment, and can be entered in the scope of the invention is set forth herein by the technology or knowledge of above-mentioned teaching or association area
Row change., then all should be in invention institute and the change and change that those skilled in the art are carried out do not depart from the spirit and scope of invention
In attached scope of the claims.
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Claims (4)
- A kind of 1. method for promoting aging Germination of Soybean Seed, it is characterised in that comprise the following steps：Step 1, Seeds preprocess：Selection size is homogeneous after choosing natural air drying, full grains, the soybean of the aging of no disease and pests harm Seed, dry 2d is returned at room temperature, it is standby；The seed that step 2, selecting step 1 obtain, puts it into the clean culture dish for being covered with double-layer filter paper；Step 3, under constant temperature non-illuminated conditions, in culture dish add diethylamino ethanol caproate solution, make seed at it Middle sprouting；Step 4, the soya seeds for handling and having sprouted by step 3 are planted in soil, photoperiod same natural environment, During emergence, appropriate running water is sprayed at regular time and quantity.
- 2. the method according to claim 1 for promoting aging Germination of Soybean Seed, it is characterised in thatThe concentration of diethylamino ethanol caproate solution is 100-400 μM in step 3.
- 3. the method according to claim 1 for promoting aging Germination of Soybean Seed, it is characterised in thatThe sprout time of seed is 12-24h in step 3.
- 4. the method according to claim 1 for promoting aging Germination of Soybean Seed, it is characterised in thatEnvironment temperature is 20-25 DEG C in step 4.
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