CN107787636B - Method for promoting aged soybean seeds to germinate - Google Patents

Abstract

The invention discloses a method for promoting aged soybean seeds to germinate, which comprises the following steps: selecting aged soybean seeds with uniform size, full grains and no plant diseases and insect pests after natural air drying, and drying at room temperature for 2d for later use; putting the selected seeds into a clean culture dish paved with double layers of filter paper; under the condition of constant temperature and no illumination, adding diethylaminoethanol caproate solution into a culture dish to germinate seeds; the germinated soybean seeds are planted in soil, the photoperiod is the same as the natural environment, and a proper amount of tap water is regularly and quantitatively sprayed during the seedling emergence period. The DA-6 of the invention has obvious promotion effect on the germination of aged soybean seeds and the subsequent photosynthesis of seedlings; provides more energy for seed germination, increases the chlorophyll content of the finished seedlings, promotes the carbon assimilation capability of plants, and finally ensures the germination of aged seeds and the photosynthetic construction of the seedlings.

Description

Method for promoting aged soybean seeds to germinate

Technical Field

The invention belongs to the field of soybean cultivation, and particularly relates to a method for promoting aged soybean seeds to germinate.

Background

Soybean (Glycine max) has a long history of cultivation in China as a protein-rich food and oil crop. China is one of important soybean production and export countries in the world in the 20 th century, the demand is continuously increased along with the rapid reduction of planting area, and the soybean is changed from export to import in China at present, thus seriously threatening the food safety in China. Therefore, the rapid development of the domestic soybean industry is a problem to be solved in the current soybean production process. However, the vigor of soybean seeds is greatly influenced by the environment, and the soybean seeds are easy to age in the storage process, so that the vigor of the seeds is rapidly reduced, and the germination and emergence are greatly influenced in agricultural production. The aging of soybean seeds seriously restricts the development of the soybean industry in China and draws wide attention.

The seed is inevitably aged after being matured, the seed coat structure is damaged, the internal stored energy substance undergoes peroxidation, and the genetic substance is damaged, so that the life of the seed is shortened. The soybean seeds are greatly influenced by the environment in the storage process and are very easy to age in the harvesting and storage processes. The preservation of soybean germplasm resources restricts the industrialization and mechanization in agricultural production, and with the development of mechanized agriculture, the requirement on the activity of crop seeds is more strict, so that the improvement of the germination rate of soybean seeds is very important.

Disclosure of Invention

In view of the above, the present invention provides a method for promoting germination of aged soybean seeds.

In order to solve the technical problem, the invention discloses a method for promoting the germination of aged soybean seeds, which comprises the following steps:

step 1, seed pretreatment: selecting aged soybean seeds with uniform size, full grains and no plant diseases and insect pests after natural air drying, and drying at room temperature for 2d for later use;

step 2, selecting the seeds obtained in the step 1, and putting the seeds into a clean culture dish paved with double layers of filter paper;

step 3, under the condition of constant temperature and no illumination, adding a diethylaminoethanol caproate solution into a culture dish to germinate seeds;

and 4, planting the germinated soybean seeds processed in the step 3 in soil, wherein the photoperiod is the same as that of the natural environment, and a proper amount of tap water is regularly and quantitatively sprayed during the seedling emergence period.

Further, the concentration of the diethylaminoethanol caproate solution in step 3 was 100-400. mu.M.

Further, the germination time of the seeds in the step 3 is 12-24 h.

Further, the ambient temperature in step 4 is 20-25 ℃.

Compared with the prior art, the invention can obtain the following technical effects:

the DA-6 of the invention has obvious promotion effect on the germination of aged soybean seeds and the subsequent photosynthesis of seedlings. The mechanism is that more energy is provided for seed germination by promoting the conversion of oil to sugar, the chlorophyll content of the established seedling is increased, the carbon assimilation capability of the plant is promoted, and finally the germination of the aged seed and the photosynthetic establishment of the seedling are ensured.

Of course, it is not necessary for any one product in which the invention is practiced to achieve all of the above-described technical effects simultaneously.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a graph of the effect of applying DA-6 during germination on germination rate of aged versus unaged soybean seeds of the present invention; wherein A, B and C are phenotype pictures at 60h in the seed germination process; d is a germination condition statistical chart of the soybean seeds under different treatment conditions; a is that the normal soybean seeds are not aged and germinate under the condition of clear water; b: aging and water are treated by aging and germinate under the condition of 0 mu M and 200 mu M of DA-6; c: aging + DA-6 is germination under 0 μ M and 200 μ M DA-6 conditions after aging treatment;

FIG. 2 is a graph of germination of normal soybean seeds of the present invention at various concentrations of DA-6; wherein CK is the germination of normal soybean seeds in clear water; 200 μ M was that normal soybean seeds germinated in 200 μ M DA-6;

FIG. 3 is the radicle length and fresh weight of the aged and virgin soybean seeds after germination for 70 hours, A is the radicle length of the soybean seeds after germination; b is the fresh weight of radicle; c is the dry weight of radicles; the unaged soybean seeds germinate under the condition of clear water; the aged + water and aged + DA-6 germinate under the conditions of 0 mu M and 200 mu M of DA-6 after aging treatment;

FIG. 4 is the seedling building capacity of aged and untreated soybean seeds of the present invention; wherein A is the establishment rate of seedlings 20 days after the germinated soybean seeds are transplanted to soil; b, establishing statistics of 20d seedlings after soybean seeds are transplanted; c is a plant height phenotype diagram after the seedlings are built; d is the statistical data of the plant height after the seedlings are built, and Bar is 5 cm; the unaged soybean seeds germinate under the condition of clear water; the aged + water and aged + DA-6 germinate under the conditions of 0 mu M and 200 mu M of DA-6 after aging treatment;

FIG. 5 is a graph of the effect of different treatments of the present invention on the traits of soybean seed seedlings after they have been established; wherein A is the difference of dry weight of the established soybean seedlings under different treatment conditions; b is the difference of chlorophyll content of the germinated seed seedlings under different treatments after the seedlings are built; the unaged soybean seeds germinate under the condition of clear water; the aged + water and aged + DA-6 germinate under the conditions of 0 mu M and 200 mu M of DA-6 after aging treatment;

FIG. 6 shows the variation of sugar content at different times of imbibition of seeds according to the invention; the change of the sucrose content of the seeds under different treatment conditions during the imbibition period; b, the change of the fructose content under different treatment conditions during the imbibition period of the seeds; c, changing the content of reducing sugar under different treatments during the imbibition period of the seeds; d, changing the content of soluble total sugar under different treatments during the imbibition period of the seeds; wherein unaged is the germination of normal soybean seeds under clear water conditions; the aged + water and aged + DA-6 germinate under the conditions of 0 mu M and 200 mu M of DA-6 after aging treatment; three histograms at each moment are sequentially unaged, aged + water and aged + DA-6 from left to right;

FIG. 7 shows the activity of sucrose invertase during imbibition of seeds of the invention; wherein the aged soybean seeds have a majority of their sucrose invertase lower than that of the unaged soybean seeds, wherein germination in clear water is very significantly different from that in DA-6 after aging, wherein unaged is that normal soybean seeds germinate under clear water conditions; the aged + water and aged + DA-6 germinate under the conditions of 0 mu M and 200 mu M of DA-6 after aging treatment; three histograms at each moment are sequentially unaged, aged + water and aged + DA-6 from left to right;

FIG. 8 is a graph showing the change in fatty acid content during the re-imbibition of seeds according to the invention; wherein B is the total amount of all fatty acids detected in the soybean seeds by GC-MS and C is the total amount of unsaturated fatty acids detected; d is the total amount of saturated fatty acids detected;

FIG. 9 shows the expression levels of genes involved in the seed imbibition process of the present invention, wherein A to D are key genes involved in the conversion of fatty acid decomposition into carbohydrates during seed germination; and E is the detection of the expression quantity of the lipoxygenase in the seed germination process.

Detailed Description

The following embodiments are described in detail with reference to the accompanying drawings, so that how to implement the technical features of the present invention to solve the technical problems and achieve the technical effects can be fully understood and implemented.

The invention discloses a method for promoting aged soybean seeds to germinate, which comprises the following steps:

step 1, seed pretreatment: selecting aged soybean seeds with uniform size, full grains and no plant diseases and insect pests (the storage life is more than 1 year, the seed coats do not mildew and completely lose the germination capacity), and drying at room temperature for 2d for later use;

step 2, selecting the seeds obtained in the step 1, and putting the seeds into a clean culture dish paved with double layers of filter paper;

step 3, under the condition of constant temperature and no illumination, adding a diethylaminoethanol caproate (DA-6) solution with the concentration of 100-;

and 4, planting the germinated soybean seeds treated in the step 3 in soil, wherein the environmental temperature is 20-25 ℃, the photoperiod is the same as the natural environment, and a proper amount of tap water is regularly and quantitatively sprayed during the seedling emergence period.

Example 1

A method of promoting germination of aged soybean seeds, comprising the steps of:

step 1, seed pretreatment: selecting aged soybean seeds with uniform size, full grains and no plant diseases and insect pests after natural air drying, and drying at room temperature for 2d for later use;

step 2, selecting the seeds obtained in the step 1, and putting the seeds into a clean culture dish paved with double layers of filter paper;

step 3, under the condition of constant temperature and no illumination, adding a diethylaminoethanol caproate (DA-6) solution with the concentration of 250 mu M into a culture dish to ensure that seeds germinate for 18 hours;

and 4, planting the germinated soybean seeds processed in the step 3 in soil, wherein the environment temperature is 22 ℃, the photoperiod is the same as the natural environment, and a proper amount of tap water is regularly and quantitatively sprayed during seedling emergence.

Example 2

A method of promoting germination of aged soybean seeds, comprising the steps of:

step 1, seed pretreatment: selecting aged soybean seeds with uniform size, full grains and no plant diseases and insect pests after natural air drying, and drying at room temperature for 2d for later use;

step 2, selecting the seeds obtained in the step 1, and putting the seeds into a clean culture dish paved with double layers of filter paper;

step 3, under the condition of constant temperature and no illumination, adding a diethylaminoethanol caproate (DA-6) solution with the concentration of 100 mu M into a culture dish to ensure that seeds germinate for 24 hours;

and 4, planting the germinated soybean seeds processed in the step 3 in soil, wherein the environment temperature is 20 ℃, the photoperiod is the same as the natural environment, and a proper amount of tap water is regularly and quantitatively sprayed during seedling emergence.

Example 3

A method of promoting germination of aged soybean seeds, comprising the steps of:

step 1, seed pretreatment: selecting aged soybean seeds with uniform size, full grains and no plant diseases and insect pests after natural air drying, and drying at room temperature for 2d for later use;

step 2, selecting the seeds obtained in the step 1, and putting the seeds into a clean culture dish paved with double layers of filter paper;

step 3, under the condition of constant temperature and no illumination, adding a diethylaminoethanol caproate (DA-6) solution with the concentration of 400 mu M into a culture dish to ensure that seeds germinate for 12 hours;

and 4, planting the germinated soybean seeds processed in the step 3 in soil, wherein the environment temperature is 25 ℃, the photoperiod is the same as the natural environment, and a proper amount of tap water is regularly and quantitatively sprayed during seedling emergence.

The technical effects of the present invention are illustrated below with reference to specific experiments:

1 materials and methods

1.1 Experimental materials

The experimental soybean variety is 'southern bean 12', and the seeds are provided by 'Sichuan province crop banded composite planting engineering technology research center' of Sichuan university of agriculture.

1.2 Experimental treatment

Carrying out artificial aging treatment on normal soybean seeds or selecting naturally aged soybean seeds, and then carrying out germination after drying for 2d at room temperature.

Taking a culture dish with the diameter of 9cm, laying two layers of medium-speed qualitative filter paper, respectively adding 20 particles of aged 12-southern-bean soybean seeds and 20 particles of unaged soybean seeds, respectively adding 20mL of DA-6 solutions with the concentrations of 100, 200, 300 and 400 mu M into the aged dish, respectively adding 20mL of ultrapure water, 200 and 400 mu M of DA-6 solutions into the unaged dish, setting 3 experimental repetitions and biological repetitions for each experiment, placing the mixture in a constant temperature box (model: Sanyo vertical Environmental Test Chamber MLR-350H) at 25 ℃ in the dark for germination for 2 days, and counting the germination rate every 12 hours during the period to determine the phenotype. The sugar content was determined at time points 0h, 12h, 24h, 36h, 48 h.

1.3 measurement index and method

1.3.1 in the experiment, the artificial accelerated aging treatment adopts a (58 +/-1 ℃) hot water accelerated artificial aging method or selects naturally aged soybean seeds, the seeds are placed in a (58 +/-1 ℃) constant-temperature water bath for treatment for 20min and then are naturally air-dried, and the germination experiment is carried out when the seeds are dried to the initial state.

1.3.2 germination rate statistics seed germinates under the dark condition in the constant temperature incubator, adopts green lamp as the light source, and statistics is sprouted at different time points, falls illumination to minimum to the influence that the seed was sprouted to the seed length of sprouting and is greater than 2mm as the judgement standard of sprouting. Radicle length was measured using Image J software and weight was weighed using an electronic balance.

1.3.3 seedling establishment statistics seeds which germinate and grow uniformly are sown in the soil, the sowing quantity in pots with the same size is the same, and the normal unearthing and the growing quantity of seedlings are counted at 7d after sowing. Plant height was determined at 20 d; the plants are removed from the soil, the rhizosphere soil is washed clean by clear water and then is sucked dry by absorbent paper, and the fresh weight is weighed by an electronic balance. Deactivating enzyme of the treated seedlings at 105 ℃ for 15min, adjusting the temperature to 75 ℃, drying to constant weight, weighing the dry weight, and respectively weighing the biomass of the whole plant.

1.3.4 measurement of chlorophyll content 3 plants of each treated seedling were selected, and the chlorophyll content was measured by extracting with 80% acetone for 24 hours according to the method of unfolding (unfolding, gavage, jujue, etc.. simplification of chlorophyll content measurement [ J ]. Physiological communication, 2010, (04):399-402.), etc.).

1.3.4 determination of different sugar content samples were taken at different time periods (0h, 12h, 24h, 36h, 48h) during seed germination, and were filled into paper bags after drying of the surface water with absorbent paper. Deactivating enzyme at 105 deg.C for 15min, oven drying at 75 deg.C to constant weight, grinding into powder in clean mortar, and placing into eppendorf tube. The content of soluble sugars was determined by the anthrone colorimetric method, reducing sugars by DNS, and the content of sucrose and fructose by the resorcinol method (Raney, Schering, Wangqinghua. the physiological effect of seed initiation on the salt tolerance of hybrid rice seedlings [ J ]. Chinese agricultural sciences, 2003,36(4): 463-468.).

1.3.5 determination of the sucrose invertase Activity samples were taken at different time periods (0h, 12h, 24h, 36h, 48h) during seed germination, snap frozen in liquid nitrogen and stored at-80 ℃. The determination method adopts a 3,5 dinitrosalicylic acid (DNS) method.

1.3.6 determination of fatty acid content samples were taken at different time periods (0h, 12h, 24h, 36h, 48h) during seed germination, snap frozen in liquid nitrogen and stored at-80 ℃. Ultrasonic extraction of fatty acid in soybean seeds by using n-hexane, screening of a Huai biological fatty acid mixed standard product in Shanghai, and analysis of components and contents of the components by Shimadzu GC-MS 2010.

1.3.7 analysis of expression of related genes Total RNA from Glycine max was extracted by the pyrogallol method, RNA integrity was determined by 1% agarose gel electrophoresis, concentration and purity were determined by Nanodrop plus, first strand cDNA synthesis was performed by Takara Bio MLV enzyme, and the detailed procedures were as described in the specification. Primer design was analyzed using the soybean phytozmev10.3 database, and NCBI sequence analysis gave the relevant soybean gene primers (Table 1). Each material was replicated 3 times with 2^-△△CTSPSS was used for significance analysis, and Microsoft Excel 2013 was used for mapping.

TABLE 1 Soybean Gene primers related thereto

1.4 statistical analysis of data

The experimental data were collated using Microsoft Excel 2013 software, the analysis of variance was SPSS 19.0 statistical analysis software, and the comparison between the mean values was performed using One-Way ANOVA; significance between the different treatments was tested using Duncan multiple comparisons and length measurements were determined using Image J.

2 results and analysis

2.1 Effect of DA-6 on Germination of aged Soybean seeds

Aged seeds germinated at different DA-6 concentrations, and it can be seen (FIGS. 1-A, B) that at 200. mu.M DA-6 concentration, it significantly increased the germination rate of aged soybean seeds. By combining the results, the concentration of 200 mu M which has great influence on the germination process of the aged seeds is selected in subsequent experiments.

DA-6 has a certain promoting effect on the germination of aged seeds, and when the DA-6 acts on normal seeds, the effect of promoting the germination rate is not found, but only the germination rate is slightly influenced during the germination process of the seeds, but the final germination rate is basically consistent with the control level (figure 2). Therefore, DA-6 only specifically improves the germination rate of the aged soybeans.

After the aged soybean seeds and the non-aged soybean seeds germinate under different treatment conditions (70h), the radicle length of the aged soybean seeds is measured by using Image J software in an auxiliary way, and the weight of the aged soybean seeds is weighed by using an electronic balance, so that the radicle length and the weight of the aged soybean seeds are obviously improved after the aged soybean seeds germinate in DA-6 compared with the germinated soybean seeds in clear water (figure 3).

2.2 influence of DA-6 on establishment of seedlings and photosynthesis after germination of aged seeds

The aged soybean seeds germinate under DA-6 condition, and the seeds with consistent growth vigor are transplanted in soil after 2d, so that the seedling establishment capacity of the aged soybean seeds germinate under DA-6 condition is obviously higher than that of seedlings germinating under clear water condition (up to 91%), and is equivalent to that of CK (soybean seeds germinating under clear water condition without aging) (FIG. 4A, B). When the control grows until the first compound leaf is completely unfolded, the plant height, fresh weight and dry weight of the control are measured, and as can be seen from fig. 4C, D, the plant height of the aged seed is remarkably improved under the condition of DA-6 with the concentration of 200 mu M, and the plant height is basically recovered to the CK level.

Further analysis of biological properties of the photomorphogenetic seedlings, respectively referring to the dry weight of the individual plants, revealed that the aged seeds formed a lower dry weight than CK, but the aged seeds germinated with DA-6 formed a significantly higher dry weight (P <0.01) than the seeds without DA-6 treatment (FIG. 5A).

Meanwhile, the aged seeds germinated in DA-6 and in clear water, and the chlorophyll content of the seeds germinated in DA-6 after the seedlings were established was significantly (P <0.05) increased compared to CK and higher than that of the unaged seeds (FIG. 5B). The fact that the content of chlorophyll can be increased by using DA-6 shows that DA-6 has a positive regulation effect on improving the seedling establishment of aged soybean seeds, and therefore DA-6 promotes the biomass accumulation to a certain extent.

2.3 influence of DA-6 on sugar content in the Germination of aged seeds

Although the sugar content of the aged seeds is reduced remarkably or extremely remarkably compared with that of the control CK in the germination process, the sucrose content of the seeds treated by DA-6 is higher than that of the seeds not treated by DA-6 (figure 6A), but the overall trend of the change shows a trend of increasing firstly and then reducing.

Like sucrose, the soluble sugars showed a tendency to increase and then decrease, but at different time points, the content after aging was always lower than that without aging, and the soluble sugar content was higher in the seeds treated with DA-6 than in the seeds without DA-6 at 12h and 24h (FIG. 6B).

2.4 DA-6 influences the enzyme activity during seed germination

During the germination process of seeds, sugar is an important component for energy supply, sucrose hydrolase is a major assimilation product of sucrose in plant tissues and is irreversibly converted into glucose and fructose, available hexose is provided for plants, energy is generated for oxidative phosphorylation coupled with respiration, and the sucrose hydrolase has close relation with the growth of the plants and can reflect the growth conditions of the plants to a certain extent.

In the experiment, the soybean seeds subjected to aging treatment germinate in DA-6 and water, the germination rates of the soybean seeds are obviously different, and the activity of the sucrose invertase in the soybean seeds is correspondingly changed. The data show that after aging treatment, sucrose turnover in the seed stage germinated in clear water was not significantly less active than the seeds germinated in DA-6 after aging. The sucrose invertase activity in the interior of the seeds germinated in DA-6 reached a level substantially comparable to that during germination of normal, spouted seeds (FIG. 7).

2.5 influence of DA-6 on the fatty acid content of aged Soybean seeds during Germination

Soybean is used as an important oil crop, a large amount of unsaturated fatty acid is stored in the soybean, during the germination of seeds, the fatty acid is decomposed and converted into saccharides which can provide energy for the growth and development of plants, and during the storage of the seeds, the fatty acid is rancid to a certain extent and is finally converted into peroxide or Malondialdehyde (MDA) of the fatty acid, so that the vitality and the germination rate of the seeds are influenced.

The data of the invention show that no matter the total amount of fatty acid, polyunsaturated fatty acid and monounsaturated fatty acid of the aged soybean seeds are totally aged and DA-6 is higher than that of the aged and water in the process of seed germination (figure 8), so that the reserve amount of the fatty acid is larger, and more sufficient energy is provided for seed germination.

2.6 Effect of DA-6 on the amount of metabolism-related Gene expression in aged Soybean seeds

During germination of the seeds, the storage material inside the seeds undergoes catabolism, providing them with the energy necessary for germination. During the seed germination process, acetyl-CoA connects three nutrient metabolism paths, enters tricarboxylic acid cycle and oxidative phosphorylation to provide energy for the seed. During the germination process of the artificially aged soybean seeds, the gene expression quantity of the seed regulation acetyl coenzyme a oxidase (ACX2) germinated in DA-6 has a remarkable up-regulation effect compared with the gene expression quantity of the seed regulation acetyl coenzyme a oxidase (ACX2) germinated in water, and simultaneously, the expression quantities of related genes SDP1 and SDP6 involved in energy metabolism are both up-regulated compared with clear water. In the gluconeogenesis pathway, the expression level of a gene (PCK1) which regulates an enzyme catalyzing oxaloacetate to form phosphoenolpyruvate and carbon dioxide also shows an up-regulation tendency. In contrast, the expression level of lipoxygenase gene (LOX3) was shown to be down-regulated in seeds germinating in DA-6 after aging, resulting in a reduction in the oxidation or peroxidation of fatty acids (FIG. 9); namely: during seed germination, DA-6 is added to enable lipolysis to be up-regulated to the expression level of key genes in a sugar process, and the expression level of LOX3 gene for regulating fatty acid oxidative degradation is down-regulated, so that fatty acid degradation is mostly converted like sugar substances, and energy is provided for seed germination.

Discussion of 3

The soybean is used as oil crop seeds, and a large amount of fatty acid is stored in the soybean, and accounts for more than 90% of the total amount of the grease. During the germination of seeds, saccharides serve as direct substrates for respiration and are reduced to various degrees during the germination of seeds. The invention shows that the contents of sucrose, reducing sugar and soluble sugar in the aging process of the soybean seeds are obviously reduced compared with the control (P <0.05), the germination rate of the aged seeds in a DA-6 solution containing 200 mu M is obviously improved (P <0.05), and the sucrose content of the aged seeds is extremely obviously improved (P <0.01) compared with the sucrose content of the aged seeds without the DA-6 treatment. This is probably because DA-6 improves the lipase activity, increases the fatty acid catabolism in soybean seeds, accelerates the conversion of lipids to sugars, provides sufficient substrate for respiration during seed germination, and ensures energy supply during seed germination.

For the aged soybean seeds, the seedling building capacity is obviously reduced compared with the control, however. The establishment rate of seedlings of aged seeds after germination in DA-6 is obviously (P <0.05) higher than that of seedlings without DA-6 treatment, and the dry weight of the established seedlings is greatly improved (P <0.01) compared with that of the seedlings without DA-6 treatment. Combined with the measurement of chlorophyll content, DA-6 treatment can significantly (P <0.05) increase chlorophyll content. The increase in dry weight is likely due to the fact that DA-6 improves the carbon assimilation ability of crops by increasing chlorophyll content, so that photosynthesis of crops is enhanced and dry matter accumulation is increased.

The invention screens out more ideal DA-6 concentration so as to improve the germination capacity, seedling building capacity and photosynthetic building capacity of the aged 'south bean 12' soybean seeds. The peroxidation level of the aged seed lipid is increased, the content of Malondialdehyde (MDA) is increased, the lipase activity is reduced, and the gluconeogenesis capability is weakened. In combination with the above data, it can be seen that DA-6 can accelerate the conversion of lipid to carbohydrate by enhancing the lipase property, so as to increase the content of soluble sugars such as sucrose, and provide direct energy for the germination of seeds. After the seeds germinate, DA-6 promotes the biosynthesis of chlorophyll, so that the chlorophyll content in the seedlings is increased, and further the photosynthesis, the light energy utilization rate and the carbon assimilation capability of the seedlings can be enhanced. Leading to the increase of the dry weight of the plant and the obvious increase of the biomass accumulation, and laying a foundation for the later growth of the plant.

While the foregoing description shows and describes several preferred embodiments of the invention, it is to be understood, as noted above, that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.

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Claims (2)

1. A method for promoting germination of aged soybean seeds, comprising the steps of:

step 1, seed pretreatment: selecting aged soybean seeds with uniform size, full grains and no plant diseases and insect pests after natural air drying, and drying at room temperature for 2d for later use;

step 2, selecting the seeds obtained in the step 1, and putting the seeds into a clean culture dish paved with double layers of filter paper;

step 3, under the condition of constant temperature and no illumination, adding 200 mu M diethylaminoethanol caproate solution into a culture dish to ensure that seeds germinate for 12-24 hours;

and 4, planting the germinated soybean seeds processed in the step 3 in soil, wherein the photoperiod is the same as that of the natural environment, and a proper amount of tap water is regularly and quantitatively sprayed during the seedling emergence period.

2. The method for promoting germination of aged soybean seeds of claim 1, wherein the ambient temperature in step 4 is 20-25 ℃.

Images