CN101283668A - Method for cultivating zinc-rich bud seeding vegetable - Google Patents
Method for cultivating zinc-rich bud seeding vegetable Download PDFInfo
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- CN101283668A CN101283668A CNA2008100534037A CN200810053403A CN101283668A CN 101283668 A CN101283668 A CN 101283668A CN A2008100534037 A CNA2008100534037 A CN A2008100534037A CN 200810053403 A CN200810053403 A CN 200810053403A CN 101283668 A CN101283668 A CN 101283668A
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Abstract
The invention relates to a cultivation method of zinc-enriched sprouting vegetables. The method comprises the following steps: soaking seeds of a sprouting vegetable in water of 25-50 DEG C for 8-12 hours, allowing germination for 2-3 days at 25-27 DEG C, spraying 10-20 mL of 1-8 mmol/L ZnSO4 solution every 4-6 hours, harvesting when the seedling is cultivated for 4-5 days and grows 6-10 cm high, and testing zinc content in the seedling. Since radish sprout is treated with ZnSO4 solution having 5 mmol/L zinc concentration, the zinc content can reach 10.02 mmol/L (daily zinc intake for normal adult is 11-20 mg). Therefore, the zinc-enriched radish sprout can supplement zinc to a person eating radish sprout that is treated with ZnSO4 solution without 5 mmol/L zinc concentration, without causing food poisoning after excessive intake. The zinc-enriched sprouting vegetable serves as convenient and safe zinc supplement.
Description
Technical field
The invention belongs to the vegetable growing technical field, relate to cultivating method nuisanceless, the noresidue vegetables, the cultivation method of the zinc-rich bud seeding vegetable of a kind of nuisanceless, noresidue of saying so more specifically.
Background technology
Bud seeding kind is a kind of novel vegetables, and it is to utilize plant seed or other nutrition organs to develop into children tender bud, seedling or stem etc. as edible a kind of special vegetable under adapt circumstance.Radish bud seeding kind eat nutraceutical on the seat of people especially particularly.The radish bud seeding kind is that radish seed is sprouted the fertile tender seedling that forms, and such seedling of gathering is direct-edible radish bud seeding kind.Japanese modern has large-scale batch production production, and each big city of China also begins to develop the production of radish bud seeding kind in succession in recent years.The radish bud seeding kind by seed in bud seedling forming process, produce the chemical change that some help edible nourishing, not only mouthfeel is fresh and tender for the plant corpus in this period, color and luster is attractive in appearance, main is mainly is the catalytic action of various hydrolases by various enzymes, insoluble big molecule reserve substance in the seed is changeed than being the simple material of solubility, thereby the contained nutriment utmost point of bud dish is beneficial to absorption of human body.The radish bud seeding kind contains amylase, helps digestion, can treat chronic gastroenteropathy; Also contain vegetable fiber, can promote enterogastric peristalsis, because sprout vegetable does not use chemical fertilizer, hormone and agricultural chemicals in process of production, therefore, it is comparatively ideal nontoxic, pollution-free, non-harmful green health care food.
Zinc is one of essential micronutrient element of crop, and scarce zinc not only can influence the growth of crop, also can influence zinc containing in making object.Chlorophyll is photosynthetic primary product, is the important indicator of photosynthetic capacity.It directly influences the photosynthetic capacity of crop, and the variation of its content is very big to the yield effect of crop, and zinc can promote the raising of chlorophyll content in the plant corpus.Zinc is the constituent of carbonic anhydrase, but and CO in the carbonic anhydrase catalysis photosynthesis of plant
2Aquation.Zinc can promote that again Vc's in the plant corpus is synthetic.But, when executing zinc, can cause murder by poisoning to plant growing when excessive again, influence the quality of bud seeding kind.At present, about bud seeding kind particularly the green turnip bud seeding kind spray behind the zinc influence to zinc content in its growth and the product, and the situation of the variation of bud seeding kind quality do not appear in the newspapers as yet, the present invention has drawn relevant result.
Summary of the invention
The object of the present invention is to provide a kind of cheap, the simple novel zinc-rich bud seeding vegetable cultivation method of method.The cultivation method of particularly rich zinc green turnip seedling dish.
The inventor finds in test: become fertile tender growth of seedlings in the phase at seed germination, spray certain density ZnSO
4The aqueous solution can make bud seeding kind zinc content increase by specific cultivation, thereby reaches the purpose that improves the rich zinc of bud seeding kind.Further research is also found: bud seeding kind applies ZnSO
4The concentration of the aqueous solution has very big difference because of the kind difference of bud seedling to the sensitivity of zinc.In order to determine ZnSO accurately
4The aqueous solution is to the concentration that applies of different bud seeding kind, the inventor has carried out a large amount of trial tests, under the prerequisite that guarantees the bud seeding kind marketing quality, further pursue bud seeding kind stem height, dry weight, fresh weight, the bud seeding kind root is long, the root fresh weight, indexs such as bud seeding kind zinc content have been finished experiment work of the present invention finally.
Zinc-rich bud seeding vegetable cultivation method of the present invention comprises:
(1) seed soaking: earlier with the bud seeding kind seed, seed soaking was handled 8~12 hours in 25~35 ℃ of water; Vernalization 2~3 days under 25~27 ℃ of temperature then;
(2) execute zinc-containing solution: spray 10~20mL, 1~8mmol.L every 4~6h
-1ZnSO
4 Solution sprays 3~5d;
(3) gather: as bud seedling cultivation 4~5d, whole strain harvesting when growing to 6~10cm is measured zinc content in the bud seeding kind bud.
The time that seed soaking of the present invention is handled is preferably 12 hours, and temperature is 27 ℃ of temperature.
Vernalization of the present invention is after more weak and intensity of illumination homogeneous area is cultivated 24h in indoor intensity of illumination, and the bud seedling is about 3000Lx and intensity of illumination vernalization 2~3 days in the zone uniformly.
Behind the bud seedling sowing 2d of the present invention, preferably spray 5~8mmol.L
-1ZnSO
4The aqueous solution.
The bud seeding kind that is rich in zinc of the present invention comprises: soya bean, mung bean, black soya bean, pea, Turnip Sprouts or green bean-leaf.Preferred Turnip Sprouts particularly preferably are green turnip seedling or mung bean bud seeding kind.
Behind the wherein preferred green turnip bud seedling sowing 2d, spray 5~8mmol.L
-1ZnSO
4The aqueous solution is more preferably and sprays 5mmol.L
-1ZnSO
4The aqueous solution.Wherein behind the mung bean sprouts seedling sowing 2d, preferably spray 8mmol.L
-1ZnSO
4The aqueous solution.
The typical green turnip bud of the present invention seedling cultivation method: spray ZnSO behind the green turnip bud seeding kind sowing 2d
4The aqueous solution, its preferred concentration is 1~8mmol.L
-1The concentration that is more preferably is 5~8mmol.L
-1, this moment, the bud seeding kind zinc content reached as high as 10.05ug/g.
Another typical mung bean sprouts seedling cultivating method of the present invention: spray ZnSO behind the sowing 2d
4The aqueous solution, its preferred concentration is 5~8mmol.L
-1, the concentration that is more preferably is 8mmol.L
-1, this moment, the bud seeding kind zinc content reached as high as 12.08ug/g.
For the actual effect of the zinc-rich bud seeding vegetable that adopts cultivation method cultivation of the present invention can more clearly be described, existing with rich zinc green turnip bud seedling cultivation and assay method as the in addition detailed description of exemplary embodiments.
The cultivation of rich zinc green turnip bud seeding kind:
(1) seed selection: select purity, cleanliness good, germination rate height, the green turnip bud seed of full seed.
(2) seed soaking: in sowing seed is cleaned, reject damage by worms, broken residual, lopsided, rotten, flat, special granule and seeds germinated.Select good green turnip seed for use, 9 parts of weighings, every part of 1g eluriates 3 times with clear water, is placed on respectively in sterilized 9 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 12h, indoor temperature is controlled at about 27 ℃ all the time.
(3) vernalization: seed is eluriated 3 times with clear water, evenly sows in seedling-cultivating tray vernalization.
(4) the compound method zinc of zinc solution is with ZnSO
4Form applies.0,2,4,6,8mmol.L zinc solution concentration is established 5 levels altogether:
-1Spray once every 4h, each 10mL sprays 3d.
(5) illumination management: behind the vernalization placing, green turnip bud seedling in indoor intensity of illumination more weak and intensity of illumination homogeneous area cultivate behind the 24h bud seedling and place indoor intensity of illumination about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) gather: whole strain harvesting when the bud seedling is cultivated 6~7d, fresh and tender, the abundant hypertrophy of cotyledon.
(8) zinc Determination on content in the green turnip bud seeding kind: according to Zhang Ping's root " the dual wavelength photometry is measured ferro-silico aluminium copper and zinc simultaneously ", physical and chemical inspection one chemical fascicle, 1997,33 (2): method is carried out in 84.Measure zinc content in the rich zinc green turnip bud seeding kind, the results are shown in Figure 1.
The cultivating method of rich zinc green turnip bud seeding kind:
(1) seed selection: select purity, cleanliness good, germination rate height, the green turnip bud seed of full seed.
(2) seed soaking: select good green turnip seed for use, 9 parts of every part of 1g of weighing eluriate 2 times with clear water, are placed on respectively in sterilized 9 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 8h.
(3) vernalization: seed is eluriated 3 times with clear water, evenly sows in seedling-cultivating tray vernalization.
(4) the compound method zinc of zinc solution is with ZnSO
4Form applies.0,1,3,5,7mmol.L zinc solution concentration is established 5 levels altogether:
-1Spray once every 4h, each 10mL sprays 3d.
(5) illumination management: behind the placing, the bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination cultivate 48h after, the bud seedling places indoor intensity of illumination about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) whole strain harvesting when the bud seedling is cultivated 7d of gathering, fresh and tender, the abundant hypertrophy of cotyledon.
(8) zinc Determination on content in the green turnip bud seeding kind: according to Zhang Ping's root " the dual wavelength photometry is measured ferro-silico aluminium copper and zinc simultaneously ", physical and chemical inspection one chemical fascicle, 1997,33 (2): method is carried out in 84.Measure zinc content in the rich zinc green turnip bud seeding kind, the results are shown in Figure 1.
The cultivating method of rich zinc green beans bud seeding kind:
(1) seed selection: select purity, cleanliness good, germination rate height, the mung bean sprouts seed of full seed.
(2) seed soaking: select good mung bean sprouts seed for use, 9 parts of weighings, every part of 2g eluriates seed 3 times with 27 ℃ clean clear water earlier, is placed on respectively in sterilized 9 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 12h.Seed soaking is eluriated seed 2 times after finishing again, and drop removes excessive moisture.
(3) vernalization: under 27 ℃ of cultivating chambers, seed is eluriated 3 times with clear water, evenly sows in seedling-cultivating tray vernalization.
(4) the compound method zinc of zinc solution is with ZnSO
4Form applies.0,2,4,6,8mmol.L zinc solution concentration is established 5 levels altogether:
-1Spray once every 4h, each 10mL sprays 3d.
(5) illumination management: behind the placing, the bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination and cultivates, and the bud seedling places the room light photograph behind the 24h, and intensity is about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) gather: when the bud seedling was cultivated 6d, 10 centimetres of heights of seedling, cotyledon launched, abundant hypertrophy.
(8) zinc Determination on content in the green turnip bud seeding kind: according to Zhang Ping's root " the dual wavelength photometry is measured ferro-silico aluminium copper and zinc simultaneously ", physical and chemical inspection one chemical fascicle, 1997,33 (2): method is carried out in 84.Measure zinc content in the rich zinc green turnip bud seeding kind, the results are shown in Figure 2.
The cultivating method of rich zinc green beans bud seeding kind:
(1) seed selection: select purity, cleanliness good, germination rate height, the mung bean sprouts seed of full seed.
(2) seed soaking: select good mung bean sprouts seed for use, 9 parts of weighings, every part of 2g eluriates seed 3 times with 27 ℃ clean clear water earlier, is placed on respectively in sterilized 9 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 12h.Seed soaking is eluriated seed 2 times after finishing again, and drop removes excessive moisture.
(3) vernalization: under 27 ℃ of cultivating chambers, seed is eluriated 3 times with clear water, evenly sows in seedling-cultivating tray vernalization.
(4) the compound method zinc of zinc solution is with ZnSO
4Form applies.0,1,3,5,7mmol.L zinc solution concentration is established 5 levels altogether:
-1Spray once every 4h, each 10mL sprays 3d.
(5) illumination management: behind the placing, the bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination and cultivates, and the bud seedling places the room light photograph behind the 24h, and intensity is about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) gather: when the bud seedling was cultivated 6d, 10 centimetres of heights of seedling, cotyledon launched, abundant hypertrophy.
(8) zinc Determination on content in the green turnip bud seeding kind: according to Zhang Ping's root " the dual wavelength photometry is measured ferro-silico aluminium copper and zinc simultaneously ", physical and chemical inspection one chemical fascicle, 1997,33 (2): method is carried out in 84.Measure zinc content in the rich zinc green turnip bud seeding kind, the results are shown in Figure 2.
Zinc is to the influence of apparent indexs such as green turnip bud seedling output
After cultivation 7d gathers, measure and spray variable concentrations ZnSO
4Stem height, dry weight, the fresh weight of solution green turnip bud seedling sample, apparent indexs such as root length and green turnip bud shoot root fresh weight, measurement result sees Table 1.
Table 1: spray variable concentrations ZnSO
4Influence to the apparent index of radish bud seeding kind plant
Annotate: n=30, p<0.05
By SPSS software each group data is analyzed, in green turnip bud seeding kind process of growth, sprayed varying level ZnSO
4Solution does not have significant difference between apparent index groups such as the plant height of the green turnip bud seedling sample after gathering, shoot dry matter, bud fresh weight, root length and root fresh weight, and each group is compared also no significant difference with blank.
Spray ZnSO
4Influence to green turnip bud seedling chlorophyll content
According to Li Hesheng chief editor " plant physiology and biochemistry experimental principle and technology ", Higher Education Publishing House, 2000.182~186,134~137.Middle method is measured and is sprayed zinc concentration 5mmol.L
-1Total chlorophyll, chlorophyll a, chlorophyll b content in the green turnip bud seedling of green turnip bud seedling leaf and blank group, and with SPSS software each is organized data and analyze, n=4, p<0.05 the results are shown in Figure 2.Abscissa represents to apply zinc concentration among the figure, the mmol.L of unit
-1, ordinate is represented chlorophyll a in the sample, chlorophyll b and total chlorophyll content, the ug.g of unit
-1, spray 5mmol.L
-1ZnSO
4Solution green turnip bud seedling is compared with blank, and chlorophyll a, chlorophyll b and total chlorophyll content all do not have significant difference.
Zinc is to the physiological influence of growing of green turnip bud seedling
According to Zhang Qide. " atmosphere CO 2 concentration raises to photosynthetic influence ", plant communication, 1992,9 (4): 18~23. and Wu Yueyan, Wang Caisheng, Wu Qiufeng " some photosynthetic physiological characteristics of grape in the overcast and rainy environment ", the gardening journal, 2005,32 (32): 387~391.Middle method is measured and is applied 5mmol.L
-1The green turnip bud seedling of zinc solution and the physical signs of blank in process of growth the results are shown in Table 2.
Table 2: spray ZnSO
4Solution is to the physiological influence of growing of radish bud seeding kind
After having listed sowing 2d in the table 2, every 48h records parameters such as photosynthesis rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, survey altogether 3 times, the sample that each concentration zinc is handled repeats 3 times, each data is mean value in the table, via SPSS software each group data is analyzed n=4, p<0.05 sprays 5mmol.L
-1ZnSO
4Solution green turnip bud seedling leaf photosynthesis rate, stomatal conductance, intercellular CO
2Concentration, transpiration rate there is no significant difference compared with the control.
Conclusion
Spray zinc concentration at 0~5mmol.L
-1In the scope, along with zinc concentration increases, the content of zinc also increases in the green turnip bud seeding kind, and zinc concentration is at 5~8mmol.L
-1In the scope, along with zinc concentration increases, the zinc content in the bud seeding kind no longer increases.By executing zinc to the influence of the apparent index of green turnip bud seeding kind, to the influence of chlorophyll content and to the interpretation of result of the influence of light and speed: the spraying concentration scope is at 0~8mmol.L
-1ZnSO
4Solution does not make significant difference to the every apparent index of green turnip bud seeding kind; Spray 5mmol.L
-1Green turnip bud seedling chlorophyll a, chlorophyll b and total chlorophyll content do not have significant difference compared with the control; Spray 5mmol.L
-1Green turnip bud seedling leaf photosynthesis rate, stomatal conductance, intercellular CO
2Concentration, transpiration rate do not have significant difference compared with the control.Illustrate that the concentration that this test is chosen can not cause zinc to poison the growth of green turnip bud seeding kind, sprays 5mmol.L in green turnip bud seeding kind cultivation process
-1Zinc solution concentration, comparatively desirable.
According to the above, the zinc solution level concentration that this experiment is chosen is reasonable, and method is feasible.According to above-mentioned analysis, concentration can be chosen 5mmol.L when applying external source zinc in green turnip bud seeding kind process of growth
-1According to international standard, the intake of normal person's zinc every day is 11~20mg, and zinc content can not surpass 50mg/100g in the food, and the radish bud seeding kind that every day, edible the inventive method was cultivated can reach certain zinc supplementation effect, even a large amount of eating can not reach toxic dose yet.Green turnip bud seeding kind of the present invention can be used as a kind of zinc supplementation food of handy and safe.
Zinc-rich bud seeding vegetable of the present invention is compared the good effect that is had with common bud seeding kind and is:
Zinc-rich bud seeding vegetable cultivating method of the present invention, it is simple to have technology, with low cost, and the output height is workable, is suitable for large-scale production, the convenient characteristics of promoting.Resulting bud seeding kind, cotyledon is fresh and tender, abundance is loose.Particularly rich zinc green turnip bud seeding kind has not only kept tasty and refreshing, the pleasant aid digestion function of common green turnip, and ought apply zinc concentration at 35mg.L
-1The time, the zinc content of green turnip bud seeding kind is 10.05ug/g, every day, edible 11~20mg can reach the effect of zinc supplementation.This has not only satisfied the demand of broad masses of the people's zinc supplementations, and the while also provides economic rich vegetables for common people's dining table.Be a kind of nothing fertilizer, no agricultural chemicals, pollution-free, nuisanceless, noresidue vegetables, have promotional value.
After the preferred embodiment that describes in detail, being familiar with this technology personage can be well understood to, can carry out various variations and modification not breaking away under above-mentioned claim and the spirit, all foundations technical spirit of the present invention all belongs to the scope of technical solution of the present invention to any simple modification, equivalent variations and modification that above embodiment did.And the embodiment that the present invention also is not subject in the specification to be given an actual example.
Description of drawings:
Fig. 1 is: zinc is in the measurement result of radish bud seeding kind in-vivo content;
Fig. 2 is: zinc is in the measurement result of mung bean bud seeding kind in-vivo content;
Fig. 3 is: zinc is to the measurement result of radish bud seeding kind chlorophyll content.Abscissa represents to apply zinc concentration among the figure, the mmol.L of unit
-1, ordinate is represented chlorophyll a in the sample, chlorophyll b and total chlorophyll content, the ug.g of unit
-1
Claims (8)
1, a kind of cultivation method of zinc-rich bud seeding vegetable, this method comprises:
(1) seed soaking: the bud seeding kind seed is soaked seed in 25~35 ℃ of water handled 8~12 hours earlier; Vernalization 2~3 days under 25~27 ℃ of temperature then;
(2) execute zinc-containing solution: spray 10~20mL, 1~8mmo l.L every 4~6h
-1ZnSO
4Solution sprays 3~5d;
(3) gather: as bud seedling cultivation 4~5d, whole strain harvesting when growing to 6~10cm is measured zinc content in the bud seeding kind bud.
2, cultivation method as claimed in claim 1, the time that wherein said seed soaking is handled is 12 hours, temperature is 27 ℃ of temperature.
3, cultivation method as claimed in claim 1, wherein said vernalization are after more weak and intensity of illumination homogeneous area is cultivated 24h in indoor intensity of illumination, and the bud seedling is in the vernalization 2~3 days in the zone uniformly of 3000Lx and intensity of illumination.
4, cultivation method as claimed in claim 1 behind the wherein said bud seedling sowing 2d, sprays 5~8mmol.L
-1ZnSO
4The aqueous solution.
5, cultivation method as claimed in claim 1, wherein said bud seeding kind comprises: soya bean, mung bean, black soya bean, pea, Turnip Sprouts or Chinese toon seedling.
6, cultivation method as claimed in claim 5, wherein said bud seeding kind is: green turnip bud seeding kind or mung bean bud seeding kind.
7, cultivation method as claimed in claim 6, the wherein said zinc-containing solution of executing is: behind the green turnip bud seedling sowing 2d, spray 5mmol.L
-1ZnSO
4The aqueous solution.
8, cultivating method as claimed in claim 6, the wherein said zinc-containing solution of executing is: behind the mung bean sprouts seedling sowing 2d, spray 8mmol.L
-1ZnSO
4The aqueous solution.
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Cited By (7)
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CN102342236A (en) * | 2010-07-26 | 2012-02-08 | 华中农业大学 | Quick production method of strontium-enriched bean sprouts |
CN103181318A (en) * | 2013-04-07 | 2013-07-03 | 苏州众仕达蔬菜食品有限公司 | Production technology of soybean sprout |
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CN102342236A (en) * | 2010-07-26 | 2012-02-08 | 华中农业大学 | Quick production method of strontium-enriched bean sprouts |
CN102342236B (en) * | 2010-07-26 | 2013-03-06 | 华中农业大学 | Quick production method of strontium-enriched bean sprouts |
CN103181318A (en) * | 2013-04-07 | 2013-07-03 | 苏州众仕达蔬菜食品有限公司 | Production technology of soybean sprout |
CN105340707A (en) * | 2015-10-29 | 2016-02-24 | 无锡苏芽食品有限公司 | Bean sprout germination method |
CN105660329A (en) * | 2016-01-15 | 2016-06-15 | 霍山县安绿园农业发展有限公司 | Method for planting zinc-rich chrysanthemum nankingense |
CN106258883A (en) * | 2016-08-08 | 2017-01-04 | 沈阳农业大学 | A kind of bud seedling vegetable zinc-iron intensifying method of glutamy sulfide |
CN109831956A (en) * | 2017-09-06 | 2019-06-04 | 重庆鼎阳繁书农业发展有限公司 | A kind of green shoot vegetable physics cultural method |
CN111264367A (en) * | 2020-01-21 | 2020-06-12 | 华南理工大学 | Edible soybean sprout rich in zinc, cobalt and selenium microelements and cultivation method thereof |
CN111264367B (en) * | 2020-01-21 | 2021-07-16 | 华南理工大学 | Edible soybean sprout rich in zinc, cobalt and selenium microelements and cultivation method thereof |
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