CN103999770B - A kind of long root induction culturing method of Chinese pine seedling - Google Patents
A kind of long root induction culturing method of Chinese pine seedling Download PDFInfo
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- CN103999770B CN103999770B CN201410211098.5A CN201410211098A CN103999770B CN 103999770 B CN103999770 B CN 103999770B CN 201410211098 A CN201410211098 A CN 201410211098A CN 103999770 B CN103999770 B CN 103999770B
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Abstract
The invention discloses the long root induction culturing method of a kind of Chinese pine seedling, the step carried out including order below: with plant growth regulator, Chinese pine seeds is carried out induction for the first time and process；Chinese pine seeds first time induction processed uniformly is sowed equipped with in the plastic film container of matrix；Chinese pine seeds germinates and terminates laggard seedling in the ranks, each plastic film container retains the nursery stock that a strain is healthy and strong, adding plant growth regulator in matrix and carry out second time induction process, wherein, described plant growth regulator is gibberellin, heteroauxin or methyl α-naphthyl acetate.This induction culturing method, combines the effect of Medium of Container Seedling and plant growth regulator, can cultivation Chinese pine seedling rapidly and efficiently, be effectively improved root growth situation, improve plant percent.
Description
Technical field
The invention belongs to forest seedling culture field, be specifically related to the long root induction culturing side of a kind of Chinese pine seedling
Method.
Background technology
Under the land occupation condition of arid climatic conditions or difficulty, dry, rainfall is few, and evaporation capacity is big, soil
Earth top layer water content is low, is easily formed dry ground layer, the moisture of dry ground layer and nutrient deficient, restricts plant growth and plants
It is resumed.Although the soil moisture content under dry ground layer is the most stable, but common plant is difficult to stretch into the most effective
Utilize moisture.
Chinese pine (Pinus tabulaeformis.) belongs to Pinaceae, Pinus needle aiphyllium, originates in China, for deeply
Root seeds, main root is flourishing, the most in depth under, side root is the most flourishing, relatively wide to surrounding horizontal stretching, because of
This has good conserving water and soil, performance of checking winds and fixing drifting sand, and is the vanguard tree seed of afforestation.
But, outstanding day by day along with Arid Problem, the seeds that Chinese pine is afforested as applicable Arid&semi-arid area,
In the soil that moisture and nutrient are seriously deficient, its deep-rootedness also cannot effectively play, and causes Chinese pine seedling to survive
Rate is low, grows up slowly, affects afforestation effect, not only cause the waste of resource manpower, also result in economic loss.
Summary of the invention
It is desirable to provide the long root induction culturing method of a kind of Chinese pine seedling, combine Medium of Container Seedling
With the effect of plant growth regulator, can cultivation Chinese pine seedling rapidly and efficiently, be effectively improved root growth
Situation, improves plant percent.This induction culturing method includes the step that order below is carried out:
1) with plant growth regulator, Chinese pine seeds is carried out induction for the first time to process；
2) Chinese pine seeds that first time induction processed uniformly is sowed equipped with in the plastic film container of matrix；
3) Chinese pine seeds germinates and terminates laggard seedling in the ranks, retains the seedling that a strain is healthy and strong in each plastic film container
Wood, adds plant growth regulator in matrix and carries out second time induction process.
Wherein, described plant growth regulator is gibberellin, heteroauxin or methyl α-naphthyl acetate.
Step 1) in, induction for the first time processes and includes that by concentration be the plant growth regulator of 25-100mg/L
Solution soaks Chinese pine seeds, and soak time is 12h, keeps soaking liquid level and exceeds seed 10cm-20cm.
Preferably, it is gibberellin that induction for the first time processes the plant growth regulator used, and concentration is 100mg/L.
Owing to gibberellin can improve the activity of seed body endoenzyme, promote that the material of endosperm storage gradates as low
Molecule solable matter, breathes for seed and organ growth provides more nutriment, thus it breaks seed
Dormancy, promotes that the effect of seed germination is even more ideal.
Step 2) in, the formula of matrix is the peat composed of rotten mosses/peat: perlite: vermiculite=4:3-4:2-3, the preferably peat composed of rotten mosses:
Perlite: vermiculite=4:4:2, the peat composed of rotten mosses: perlite: vermiculite=4:3:3 or peat: perlite: vermiculite=4:4:2
In one, the more preferably peat composed of rotten mosses: perlite: vermiculite=4:3:3.
The peat composed of rotten mosses or peat permeability are good, rich in nutrition content；Vermiculite moisture retention is good, beneficially seedling planting；Pearl
Petrosal foramen porosity is high, good permeability, beneficially plant root growth and growth；Three mixes with aforementioned proportion, its
Porosity and air water ratio are moderate, have fertilizer conservation water retention simultaneously, are suitable for the cultivation of Chinese pine long root nursery stock.
Particularly, matrix, with after formalin solution spray disinfectant, loads in plastic film container, formalin
The concentration of solution is 40%.
Especially, on plastic film container bore be 10-20cm, the degree of depth be 40-50cm.This plastic sheeting holds
Device is compared with conventional vessel, and the degree of depth is deeper, more conducively the holding and the growth of long root seedling root of matrix.
Particularly, the sowing number in each plastic film container is 25-35 grain.
Step 3) in, induction for the second time processes and is included in described matrix interpolation concentration is 25-100mg/L's
Plant growth regulator solution.
Particularly, the consumption of the plant growth regulator in each plastic film container is 150-250ml, to water
The most all matrix is advisable.
Preferably, it is methyl α-naphthyl acetate that induction for the second time processes the plant growth regulator used, and concentration is 50mg/L.
Methyl α-naphthyl acetate can promote cell elongation, and affects the expression at the tip of a root of the plant tumor growth element related gene,
It promotes that the effect of Chinese pine seedling long root growth is the most notable.
Wherein, this induction culturing method, before being additionally included in the process of induction for the first time, with liquor potassic permanganate to oil
Pine seed disinfection, and rinse with clear water, remove empty grain and flat grain seed.Liquor potassic permanganate dense
Degree is 0.5%.
The present invention compared with prior art, has a following beneficial outcomes:
1, the induction culturing method of the present invention uses plastic film container nursery, does not has limit season, is beneficial to control
Breeding condition processed, can transplant with carrying substrates, has and does not hinders the feature that root, seedling-slowing stage are short, and, moulding of selection
The material more conventional container depth of membrane container is deeper, is more suitable for the cultivation of long root nursery stock；
2, the induction culturing method of the present invention does not use general loess to cultivate as matrix, but have selected
Be conducive to root growth, there is the peat composed of rotten mosses or peat, perlite, the vermiculite of preservation capacity of water and fertility energy, with the peat composed of rotten mosses/mud
Charcoal: perlite: the proportioning configuration of vermiculite=4:3-4:2-3, its porosity and air water ratio are moderate, have fertilizer conservation simultaneously
Water retention, is suitable for Chinese pine and cultivates；
3, the induction culturing method of the present invention has carried out twice induction process, and it is at seed that induction for the first time processes
Development stage uses plant growth regulator solution seed soaking, and it is to use in the growth of seedling stage that induction for the second time processes
Plant growth regulator solution pouring root, twice induction processes the effect coordinating matrix again, farthest promotes
The germination of Chinese pine seeds and the growth of root system；
4, using the induction culturing method of the present invention compared with conventional treatment method, the Chinese pine seeds cultivated is sent out
Bud rate improves more than 18%, and germinating energy and dry weight the most all significantly improve；The Chinese pine seedling cultivated, its root
Being more than extension 30cm, root surface area increases 10cm2Above, tip of a root number increases more than 100, its with
The contact area of soil is greatly increased, thus can grow downwards after afforestation, gos deep into soil, effectively utilizes
Moisture in profound soil and nutrient, the minor betterment primary growth environment of plant, it is easier to survive.
Detailed description of the invention
The beneficial effect of technical scheme of the present invention is further described below by detailed description of the invention, these
Embodiment is only exemplary, the scope of the present invention is not constituted any restriction.Those skilled in the art should
It is understood by, can thin to technical solution of the present invention under without departing from the formula thinking of the present invention, purposes scope
Joint and form are modified or replace, but these amendments and replacement each fall within protection scope of the present invention.
Embodiment 1
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the gibberellin (GA that concentration is 100mg/L3) Chinese pine seeds after solution dipping pretreatment, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:3:3
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 15cm, the degree of depth be 45cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 30 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in methyl α-naphthyl acetate (NAA) solution 200ml that concentration is 50mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 2
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the gibberellin (GA that concentration is 50mg/L3) Chinese pine seeds after solution dipping pretreatment, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:3:3
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 10cm, the degree of depth be 40cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 25 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in methyl α-naphthyl acetate (NAA) solution 150ml that concentration is 25mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 3
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the gibberellin (GA that concentration is 100mg/L3) Chinese pine seeds after solution dipping pretreatment, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:3:3
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 15cm, the degree of depth be 45cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 30 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in methyl α-naphthyl acetate (NAA) solution 200ml that concentration is 50mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 4
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the gibberellin (GA that concentration is 25mg/L3) Chinese pine seeds after solution dipping pretreatment, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:3:3
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 10cm, the degree of depth be 50cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 25 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in methyl α-naphthyl acetate (NAA) solution 175ml that concentration is 100mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 5
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the Chinese pine seeds after methyl α-naphthyl acetate (NAA) the solution dipping pretreatment that concentration is 25mg/L, during immersion
Between be 12 hours, keep soak liquid level exceed seed 15cmm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 20cm, the degree of depth be 40cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 35 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in heteroauxin (IAA) solution 225ml that concentration is 50mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 6
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the gibberellin (GA that concentration is 50mg/L3) Chinese pine seeds after solution dipping pretreatment, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 20cm, the degree of depth be 50cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 35 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in methyl α-naphthyl acetate (NAA) solution 1000ml that concentration is 25mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 7
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the Chinese pine seeds after heteroauxin (IAA) the solution dipping pretreatment that concentration is 25mg/L, during immersion
Between be 12 hours, keep soak liquid level exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 10cm, the degree of depth be 40cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 25 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the gibberellin (GA that concentration is 25mg/L3) solution 150ml pouring matrix in root,
Carry out second time induction to process.
Embodiment 8
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the gibberellin (GA that concentration is 50mg/L3) Chinese pine seeds after solution dipping pretreatment, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 15cm, the degree of depth be 40cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 30 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in methyl α-naphthyl acetate (NAA) solution 200ml that concentration is 25mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 9
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the gibberellin (GA that concentration is 100mg/L3) Chinese pine seeds after solution dipping pretreatment, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select commercially available peat, perlite and vermiculite, according to peat: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 10cm, the degree of depth be 40cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 25 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in methyl α-naphthyl acetate (NAA) 150ml solution that concentration is 50mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 10
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the Chinese pine seeds after methyl α-naphthyl acetate (NAA) the solution dipping pretreatment that concentration is 25mg/L, during immersion
Between be 12 hours, keep soak liquid level exceed seed 15cm.
3, matrix treatments:
Select commercially available peat, perlite and vermiculite, according to peat: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 15cm, the degree of depth be 45cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 30 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in heteroauxin (IAA) 200ml solution that concentration is 100mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 11
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the Chinese pine seeds after methyl α-naphthyl acetate (NAA) the solution dipping pretreatment that concentration is 100mg/L, during immersion
Between be 12 hours, keep soak liquid level exceed seed 15cm.
3, matrix treatments:
Select commercially available peat, perlite and vermiculite, according to peat: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 10cm, the degree of depth be 50cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 25 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in heteroauxin (IAA) solution 175ml that concentration is 25mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 12
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the Chinese pine seeds after heteroauxin (IAA) the solution dipping pretreatment that concentration is 100mg/L, during immersion
Between be 12 hours, keep soak liquid level exceed seed 15cm.
3, matrix treatments:
Select commercially available peat, perlite and vermiculite, according to peat: perlite: the proportional arrangement of vermiculite=4:4:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 20cm, the degree of depth be 50cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 35 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the root in heteroauxin (IAA) the solution 250ml of concentration 100mg/L pouring matrix,
Carry out second time induction to process.
Embodiment 13
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
With the Chinese pine seeds after heteroauxin (IAA) the solution dipping pretreatment that concentration is 50mg/L, during immersion
Between be 12 hours, keep soak liquid level exceed seed 15cm.
3, matrix treatments:
Select the commercially available peat composed of rotten mosses, perlite and vermiculite, according to the peat composed of rotten mosses: perlite: the proportional arrangement of vermiculite=4:4:3
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 10cm, the degree of depth be 40cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 25 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the gibberellin (GA that concentration is 100mg/L3) 150ml solution pouring matrix in root,
Carry out second time induction to process.
Embodiment 14
1, pretreatment:
Soaking Chinese pine seeds with the liquor potassic permanganate that concentration is 0.5%, soak time is 30min, keeps leaching
Bubble liquid level exceeds seed 15cm.
Pull out after immersion, rinse 3 times with clear water, remove empty grain and flat grain seed.
2, induction for the first time processes
By the Chinese pine seeds after methyl α-naphthyl acetate (NAA) the solution dipping pretreatment that concentration is 50mg/L, soak time
It is 12 hours, keeps soaking liquid level and exceed seed 15cm.
3, matrix treatments:
Select commercially available peat, perlite and vermiculite, according to peat: perlite: the proportional arrangement of vermiculite=4:3:2
Matrix, the formalin solution with 40% sprays matrix and carries out disinfection；
Matrix after sterilization is added upper bore be 10cm, the degree of depth be 40cm plastic film container in, to containing
The matrix put, at upper opening of container edge 2cm, uniformly sows 25 Chinese pine seeds.
4, managing after sowing:
Take manually to water, depending on its irrigation amount and time gap length should be according to the temperature on the same day, intensities of illumination,
Cloudy day and rainy day reduce irrigation amount or do not water；And with 0.5% carbendazim solution spraying controlling disease, artificial removal
Weeds.
5, induction for the second time processes:
Terminate through 30 days germinations, carry out thinning, the most each plastic film container retains a strain healthy and strong
Nursery stock, then with the gibberellin (GA that concentration is 50mg/L3) solution 150ml pouring matrix in root,
Carry out second time induction to process.
Reference examples 1
Soak Chinese pine seeds 12h with warm water, sow after having 15% seed to start to show money or valuables one carries unintentionally, choose commercially available Huang
Soil (picking up from Wuqi County, Shaanxi Province) is matrix, puts into plastic film container same as in Example 1, uniformly
Sow 30 Chinese pine seeds.Managing after sowing is same as in Example 1.
Reference examples 2
Soak Chinese pine seeds 12h with warm water, sow after having 15% seed to start to show money or valuables one carries unintentionally, choose matrix and join
Than the peat composed of rotten mosses: perlite: the matrix of vermiculite=4:3:3, put into plastic film container same as in Example 1,
Uniformly 30 Chinese pine seeds of sowing.Managing after sowing is same as in Example 1.
Reference examples 3
Soak Chinese pine seeds 12h with warm water, sow after having 15% seed to start to show money or valuables one carries unintentionally, choose matrix and join
Than peat: perlite: the matrix of vermiculite=4:4:2, put into plastic film container same as in Example 1,
Uniformly 30 Chinese pine seeds of sowing.Managing after sowing is same as in Example 1.
Test example 1 Chinese pine seeds germination
1. test method
After planting the germination of the Chinese pine seeds that embodiment and reference examples are cultivated is observed.Wherein, broadcast
Measure germinating energy after planting 7 days, after 21 days, measure germination percentage.
Seed number x100% is planted experimentally in germination percentage=chitting piece grain number/confession
Seed number x100% is planted experimentally in germinating energy=chitting piece grain number/confession.
Note: germination percentage, germinating energy owing to all first passing through arcsin function conversion for percentage data, then carries out data analysis, phase
List in the form of pass is all the data after conversion.
2. result of the test
The germination of table 1 Chinese pine seeds
| Germination percentage (%) | Germinating energy (%) | Dry weight (g/plant) | |
| Reference examples 1 | 49.03 | 34.43 | 0.504 |
| Reference examples 2 | 54.97 | 36.03 | 0.541 |
| Reference examples 3 | 55.07 | 36.06 | 0.556 |
| Embodiment 1 | 74.79 | 43.28 | 0.904 |
| Embodiment 2 | 67.60 | 40.97 | 0.826 |
| Embodiment 3 | 70.47 | 43.28 | 0.887 |
| Embodiment 4 | 65.06 | 39.77 | 0.871 |
| Embodiment 5 | 62.14 | 38.61 | 0.727 |
| Embodiment 6 | 67.60 | 40.97 | 0.622 |
| Embodiment 7 | 66.02 | 39.05 | 0.705 |
| Embodiment 8 | 62.97 | 39.22 | 0.747 |
| Embodiment 9 | 65.76 | 40.38 | 0.622 |
| Embodiment 10 | 63.25 | 40.39 | 0.656 |
| Embodiment 11 | 61.40 | 38.44 | 0.716 |
| Embodiment 12 | 62.14 | 38.03 | 0.590 |
| Embodiment 13 | 57.97 | 38.86 | 0.596 |
| Embodiment 14 | 58.08 | 38.63 | 0.591 |
As shown in Table 1, the Chinese pine seeds germination percentage that reference examples 1 processes is less than 50%, and dry weight is 0.504g；Right
After selecting matrix of the present invention to process in 2 and 3 as usual, germination percentage improves to about 55%, and dry weight carries
Up to 0.54-0.56g；Carry out embodiment 1-14 processed with induction culturing method of the present invention, germination percentage exists
More than 57.97%, dry weight, at more than 0.59g, is better than reference examples 1-3.Especially embodiment 1-4, uses preferably
Plant growth regulator and matrix process, germination percentage reaches more than 65%, dry weight at more than 0.82g,
Advantage becomes apparent from.Germinating energy is also demonstrated by the most close improvement trend.
Visible, matrix treatments has certain facilitation to the germination of Chinese pine seeds, and uses plant of the present invention
The induction culturing method of the processing mode that growth regulator and matrix combine, compared with reference examples 1, germination percentage
Improving more than 18%, germinating energy and dry weight also have a certain degree of increasing, and the promotion to the germination of Chinese pine seeds is made
With the most notable.
Test example 2 Chinese pine seedlings root growing state
1. test method
Respectively after sowing 4 months and 12 months, the Chinese pine seedling that embodiment and reference examples are cultivated
Root growth situation is observed.After removing root matrix, wash, use WinRHIZO root system to sweep
Retouch analyzer scanning root system image, be analyzed measuring root length, root system surface area, the tip of a root with analysis software
Number.
The root growth situation of table 2 Chinese pine seedling
As shown in Table 2, the Chinese pine growth of seedling that reference examples 1 is cultivated is after 12 months, and root length is
363.903cm, root surface area is 102.609cm2, tip of a root number is 611.75；In reference examples 2 and 3, select
After matrix of the present invention processes, growth of seedling is after 12 months, and root length brings up to more than 387cm,
Root surface area brings up to 106cm2Above, tip of a root number brings up to more than 630；With induction culturing of the present invention
Method carries out embodiment 1-14 processed, and growth of seedling is after 12 months, root length bring up to 396cm with
On, root surface area brings up to 112cm2Above, tip of a root number brings up to more than 730, is better than reference examples 1-3；Especially
It is embodiment 1-4, uses preferred plant growth regulator and matrix to process, growth of seedling 12 months
After root length bring up to 129cm at more than 430cm, root surface area2Above, tip of a root number brings up to 820
Above, advantage becomes apparent from.
Visible, use matrix treatments can promote growth of seedling to a certain extent, play its long root, and use this
The induction culturing method of the processing mode that invention plant growth regulator and matrix combine, the effect of promotion is more
Substantially, the Chinese pine seedlings root length cultivated increases more than 30cm compared with reference examples 1, and root surface area increases
Add 10cm2Above, tip of a root number increases more than 100, thus is more conducive to seedling growth downwards after afforestation,
Go deep into soil, utilize the moisture in profound soil and nutrient.
Claims (5)
1. the long root induction culturing method of a Chinese pine seedling, it is characterised in that include that order below is carried out
Step:
1) with plant growth regulator to will the Chinese pine seeds of nursery in a reservoir carry out at induction for the first time
Reason；
2) Chinese pine seeds that first time induction processed uniformly is sowed equipped with comprising the peat composed of rotten mosses/peat, pearl
In the plastic film container of the matrix of rock and vermiculite；
3) Chinese pine seeds germinates and terminates laggard seedling in the ranks, retains the seedling that a strain is healthy and strong in each plastic film container
Wood；
4), after thinning, the matrix comprising the peat composed of rotten mosses/peat, perlite and vermiculite is added plant growth regulating
Agent carries out second time induction and processes, and obtains the Chinese pine seedling of long root；
Wherein, described second time induction processes and is included in described matrix interpolation concentration is the plant of 25-100mg/L
Growth regulating agent solution, described plant growth regulator is gibberellin or heteroauxin；
Wherein, step 1) described in for the first time induction process and include: raw with the plant that concentration is 25-100mg/L
Long conditioning agent solution soaks Chinese pine seeds, and soak time is 12h, and described plant growth regulator is heteroauxin
Or methyl α-naphthyl acetate；
Wherein, the formula of described matrix is the peat composed of rotten mosses/peat: perlite: vermiculite=4:3-4:2-3.
2. induction culturing method as claimed in claim 1, it is characterised in that step 2) described in matrix
Formula is the peat composed of rotten mosses: perlite: vermiculite=4:4:2, the peat composed of rotten mosses: perlite: vermiculite=4:3:3 or peat: precious
Zhu Yan: the one in vermiculite=4:4:2.
3. induction culturing method as claimed in claim 1, it is characterised in that step 2) described in matrix use
After formalin solution spray disinfectant, load in plastic film container.
4. the induction culturing method as described in claim 1 or 3, it is characterised in that step 2) described in plastics
The upper bore of membrane container is 10-20cm, the degree of depth is 40-50cm.
5. induction culturing method as claimed in claim 1, it is characterised in that be additionally included at induction for the first time
Before reason, with liquor potassic permanganate to Chinese pine seeds disinfection, and rinse with clear water, remove empty grain and flat
Grain seed.
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