CN115245175B - Seed treating agent and preparation method and application thereof - Google Patents
Seed treating agent and preparation method and application thereof Download PDFInfo
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- CN115245175B CN115245175B CN202211045277.7A CN202211045277A CN115245175B CN 115245175 B CN115245175 B CN 115245175B CN 202211045277 A CN202211045277 A CN 202211045277A CN 115245175 B CN115245175 B CN 115245175B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
- C05C5/04—Fertilisers containing other nitrates containing calcium nitrate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The invention provides a seed treating agent, a preparation method and application thereof, and belongs to the technical field of crop planting. The invention provides a seed treating agent, which comprises calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is (35-40): (2-4): (1-2): (1-2). The seed treatment agent provided by the invention is composed of the regulating substances and the special nutrient substances, and the components can play a role in synergy through reasonable compatibility and combination, have the effects of remarkably improving the nutrition of seeds and improving the resistance of the seeds, and further help to play the effects of promoting the germination of the seeds and improving the properties of seedlings. The seed treatment agent provided by the invention can be used for treating seeds, can improve the planting efficiency of crops, and is suitable for being widely applied to the technical field of crop planting.
Description
Technical Field
The invention belongs to the technical field of crop planting, and particularly relates to a seed treating agent, a preparation method and application thereof.
Background
Peanuts are rich in grease and protein, are important oil sources and raw materials for the food industry, and play an important role in daily life of the national people. The production level of peanuts also affects the living standard of people and the socioeconomic state of development, and has important promotion effect on the improvement of agricultural production.
In peanut cultivation, high-yield stable yield of peanuts plays an important role in the cultivation technology except that high-quality peanut varieties are selected, and in the cultivation technology, seed soaking treatment is adopted, so that the improvement of seed germination and seedling quality is a key factor for yield increase.
Peanut seed soaking treatment is one of the common measures in peanut cultivation, and in recent years, peanut seed soaking agents are rapidly developed, and research and application in aspects of preventing and controlling peanut soil insects and regulating seed vigor are mainly focused. At present, peanut seed soaking agents aim at more seed treatments of peanut underground pests, the seed soaking agents in the aspect of regulating and controlling the activity of the seeds are relatively less, and most seed soaking agents for regulating and controlling the activity of the seeds are single element seed soaking agents, so that the effect is relatively single, and the peanut seed soaking requirements cannot be met. For example, the germination rate of peanut seed soaking agent treated seeds is to be increased; or the peanut seed soaking agent can promote seed germination, and has no obvious influence on the growth of seedlings; or the peanut seed soaking agent is used for treating seeds, the emergence speed is slow, the phenomenon of big and small seedlings is serious, the uniformity is not high, and the like.
Disclosure of Invention
The invention aims to provide a seed treating agent, which can promote peanut seed germination, improve peanut seed germination rate, promote seedling growth, improve seedling quality, and ensure stable seedling property and high uniformity.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a seed treatment agent, which comprises the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is (35-40): (2-4): (1-2): (1-2).
Preferably, each liter of seed treatment comprises the following components in mass: 35-40 g of calcium nitrate, 2-4 g of magnesium sulfate, 1-2 g of leucine and 1-2 g of glycine; the seed treatment agent takes water as a dispersion medium.
The invention provides a preparation method of the seed treatment agent, which comprises the following steps:
mixing calcium nitrate with water to obtain a calcium nitrate solution;
mixing the calcium nitrate solution with leucine, and standing to obtain an initial mixed solution;
mixing the initial mixed solution with magnesium sulfate to obtain a second mixed solution;
and mixing the second mixed solution with glycine to obtain the seed treating agent.
Preferably, the time of the standing is 20 to 26 hours.
Preferably, after the second mixed solution is mixed with glycine, the seed treating agent is obtained by fixing the volume.
The invention provides application of the seed treatment agent in peanut seed soaking.
Preferably, the application comprises: the germination rate of peanut seeds is improved through the seed soaking of the peanuts.
Preferably, the application comprises: and improving the quality of seedlings by immersing the peanut seeds.
The invention also provides a use method of the seed treatment agent in the technical scheme, and the seed treatment agent is diluted by 100 times and then soaked.
Preferably, the seed treatment agent is used for soaking seeds for 12-18 hours.
The beneficial effects are that:
the seed treating agent provided by the invention comprises the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is (35-40): (2-4): (1-2): (1-2). The seed treatment agent provided by the invention consists of calcium nitrate, magnesium sulfate, leucine and glycine, the components consist of regulating substances and special nutrient substances, and the components can play a synergistic effect through reasonable compatibility and combination, so that the seed treatment agent has the effects of remarkably improving the nutrition of seeds and improving the resistance of the seeds. The seed treatment agent can promote peanut seed germination and improve peanut seed germination rate; meanwhile, the growth of seedlings can be promoted, the quality of the seedlings is enhanced, and the seedlings are stable in property and high in uniformity. The results of the examples show that: the seed treatment agent provided by the invention is used for carrying out seed soaking treatment on peanut seeds for more than 12 hours by diluting 100 times of liquid with water, so that the germination potential of the seeds can be improved, the germination rate is increased by 8.62% compared with that of a blank control on average, and the germination rate is increased by 11.25% compared with that of the blank control on average; the fresh weight of the aerial parts of the corn seedlings is increased by 9.45 percent compared with that of the blank control, and the dry weight is increased by 20.25 percent compared with that of the blank control; the fresh weight of the root system is increased by 54.55% compared with the blank control, the dry weight is increased by 66.67% compared with the blank control, and the activity of the root system is increased by 44.44% compared with the blank control.
Detailed Description
The invention provides a seed treatment agent, which comprises the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is (35-40): (2-4): (1-2): (1-2).
The source of the components of the present invention is not particularly limited, and may be conventionally used commercially in the art unless otherwise specified.
The seed treatment agent provided by the invention comprises 35-40 parts by mass of calcium nitrate, more preferably 35-38 parts by mass, and even more preferably 35 parts by mass. In the present invention, the seed treatment agent preferably contains 35 to 40g, more preferably 35 to 38g, and still more preferably 35g of calcium nitrate per liter of seed treatment agent. In the invention, the calcium nitrate is used as an initiator, so that the cell osmotic potential can be reduced, the water absorption of cells in seeds can be accelerated and promoted, and the salt metabolism and the activity can be promoted.
The seed treatment agent provided by the invention comprises 2-4 parts of magnesium sulfate, and more preferably 3-4 parts of magnesium sulfate based on the mass parts of calcium nitrate. In the present invention, 2 to 4g, more preferably 3 to 4g of magnesium sulfate is preferably contained per liter of the seed treatment agent. In the invention, the magnesium sulfate has the capability of improving nutrient absorption of plants, and can promote root growth and repair.
The seed treatment agent provided by the invention comprises 1-2 parts of leucine, and more preferably 1.5 parts of leucine based on the mass parts of calcium nitrate. In the present invention, leucine is preferably contained in an amount of 1 to 2g, more preferably 1.5g, per liter of the seed treatment agent. In the invention, the effect of leucine chelates calcium ions, and has the effect of promoting the root system development of seed seedlings and the capability of resisting salt stress.
The seed treatment agent provided by the invention comprises 1-2 parts of glycine, and more preferably 1.5 parts of glycine based on the mass parts of calcium nitrate. In the present invention, glycine is preferably contained in an amount of 1 to 2g, more preferably 1.5g, per liter of the seed treatment agent. In the invention, the efficacy of glycine can increase the absorption of seedling root system to phosphorus and potassium elements, improve stress resistance, and finally have the effect of chelating calcium ions and magnesium ions in the seed treatment agent, and improve the stability of the seed treatment agent.
In the present invention, the seed treatment agent uses water as a dispersion medium.
In the invention, the seed treatment agent comprises calcium nitrate, magnesium sulfate, leucine and glycine, the components consist of regulating substances and special nutrient substances, the components are reasonably combined to obtain the composite agent with stable properties, the components in the composite agent can play a role in synergy, the effects of remarkably improving the nutrition of seeds and improving the resistance of the seeds are achieved, and the effects of promoting the germination of the seeds and improving the quality of seedlings are further achieved.
The embodiment of the invention provides a seed treatment agent, which consists of the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is 35:2:1:1.
the embodiment of the invention provides a seed treatment agent, which consists of the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is 35:4:2:2.
the embodiment of the invention provides a seed treatment agent, which consists of the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is 40:2:1:1.
the embodiment of the invention provides a seed treatment agent, which consists of the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is 40:4:2:2.
the embodiment of the invention provides a seed treatment agent, which consists of the following components: calcium nitrate, magnesium sulfate, leucine and glycine; the mass ratio of the calcium nitrate to the magnesium sulfate to the leucine to the glycine is 38:3:1.5:1.5.
the invention provides a preparation method of the seed treatment agent, which comprises the following steps:
mixing calcium nitrate with water to obtain a calcium nitrate solution;
mixing the calcium nitrate solution with leucine, and standing to obtain an initial mixed solution;
mixing the initial mixed solution with magnesium sulfate to obtain a second mixed solution;
and mixing the second mixed solution with glycine to obtain the seed treating agent.
The invention mixes calcium nitrate with water to obtain calcium nitrate solution. The method for mixing the calcium nitrate and the water is not particularly limited, and a mixing method conventional in the art can be adopted. In the invention, the mixing mode of mixing the calcium nitrate and the water is specifically to dissolve the calcium nitrate in a proper amount of water to fully dissolve the calcium nitrate, so as to obtain a calcium nitrate solution.
After the calcium nitrate solution is obtained, the calcium nitrate solution is mixed with leucine and is stood still to obtain an initial mixed solution. The method for mixing the calcium nitrate solution and leucine is not particularly limited, and the conventional mixing method in the field can be adopted. In the invention, the mode of mixing the calcium nitrate solution and leucine is specifically to add leucine into the calcium nitrate solution so as to fully dissolve the leucine. In the present invention, the time for the standing is preferably 20 to 26 hours, more preferably 24 hours. The standing is for sufficient chelation of both calcium nitrate and leucine.
After the initial mixed solution is obtained, the initial mixed solution is mixed with magnesium sulfate to obtain a second mixed solution. The method of mixing the initial mixture and magnesium sulfate is not particularly limited, and a conventional mixing method in the art may be adopted. In the invention, the mode of mixing the initial mixed solution and the magnesium sulfate is specifically that the magnesium sulfate is added into the initial mixed solution to be fully dissolved.
After the second mixed solution is obtained, the second mixed solution is mixed with glycine to obtain the seed treating agent. The mode of mixing the second mixed solution and glycine is not particularly limited, and a mixing mode conventional in the art can be adopted. In the invention, the mode of mixing the second mixed solution and glycine is specifically that glycine is added into the second mixed solution to be fully dissolved. In the present invention, after the second mixed solution is mixed with glycine, the seed treatment agent is obtained preferably by further comprising constant volume. The constant volume is preferably between 1000mL.
When the seed treatment agent is prepared, the addition sequence of each raw material is strictly controlled, so that the stability of the seed treatment agent can be ensured, no precipitation is generated, and the prepared seed treatment agent is more stable and can effectively play roles.
The invention provides application of the seed treatment agent in peanut seed soaking.
In the present invention, the use of the seed treatment in the soaking of peanut seeds preferably comprises increasing the germination rate of peanut seeds by the soaking of peanut seeds. In the present invention, the use of the seed treatment in the soaking of peanut seeds preferably comprises improving seedling quality by the soaking of peanut seeds.
The invention also provides a use method of the seed treatment agent in the technical scheme, and the seed treatment agent is diluted by 100 times and then soaked. In the present invention, the time for seed soaking is preferably 12 to 18 hours, more preferably 16 hours. The invention is not particularly limited to the seed soaking temperature, and the temperature used for conventional seed soaking can be adopted.
In order to further illustrate the present invention, the seed treatment provided in the present invention, and the preparation method and application thereof are described in detail with reference to the following examples, which are not to be construed as limiting the scope of the present invention.
Example 1
The seed treating agent is prepared by the following preparation method:
dissolving 35g of calcium nitrate in a proper amount of water, and obtaining a calcium nitrate solution after the calcium nitrate is fully dissolved;
adding 1g of leucine into the calcium nitrate solution, fully dissolving and standing for 24 hours to obtain an initial mixed solution;
adding 2g of magnesium sulfate into the initial mixed solution, and fully dissolving and mixing to obtain a second mixed solution;
adding 1g glycine into the second mixed solution, adding water to dissolve to 1000ml, and fully dissolving and mixing to obtain the seed treating agent.
Example 2
The seed treating agent is prepared by the following preparation method:
dissolving 35g of calcium nitrate in a proper amount of water, and obtaining a calcium nitrate solution after the calcium nitrate is fully dissolved;
adding 2g of leucine into the calcium nitrate solution, fully dissolving and standing for 24 hours to obtain an initial mixed solution;
adding 4g of magnesium sulfate into the initial mixed solution, and fully dissolving and mixing to obtain a second mixed solution;
adding 2g glycine into the second mixed solution, adding water to dissolve to 1000ml, and fully dissolving and mixing to obtain the seed treating agent.
Example 3
The seed treating agent is prepared by the following preparation method:
dissolving 40g of calcium nitrate in a proper amount of water, and obtaining a calcium nitrate solution after the calcium nitrate is fully dissolved;
adding 1g of leucine into the calcium nitrate solution, fully dissolving and standing for 24 hours to obtain an initial mixed solution;
adding 2g of magnesium sulfate into the initial mixed solution, and fully dissolving and mixing to obtain a second mixed solution;
adding 1g glycine into the second mixed solution, adding water to dissolve to 1000ml, and fully dissolving and mixing to obtain the seed treating agent.
Example 4
The seed treating agent is prepared by the following preparation method:
dissolving 40g of calcium nitrate in a proper amount of water, and obtaining a calcium nitrate solution after the calcium nitrate is fully dissolved;
adding 2g of leucine into the calcium nitrate solution, fully dissolving and standing for 24 hours to obtain an initial mixed solution;
adding 4g of magnesium sulfate into the initial mixed solution, and fully dissolving and mixing to obtain a second mixed solution;
adding 2g glycine into the second mixed solution, adding water to dissolve to 1000ml, and fully dissolving and mixing to obtain the seed treating agent.
Example 5
The seed treating agent is prepared by the following preparation method:
dissolving 38g of calcium nitrate in a proper amount of water, and obtaining a calcium nitrate solution after the calcium nitrate is fully dissolved;
adding 1.5g leucine into the calcium nitrate solution, fully dissolving and standing for 24 hours to obtain an initial mixed solution;
adding 3g of magnesium sulfate into the initial mixed solution, and fully dissolving and mixing to obtain a second mixed solution;
adding 1.5g glycine into the second mixed solution, adding water to dissolve to 1000ml, and fully dissolving and mixing to obtain the seed treating agent.
Comparative example 1
A seed treating agent consists of 400mg/L calcium nitrate solution.
Comparative example 2
A seed treatment agent consists of 40mg/L magnesium sulfate solution.
Comparative example 3
A seed treatment agent comprises leucine solution 20 mg/L.
Comparative example 4
A seed treatment agent consists of 20mg/L glycine solution.
Example 6
Seed treatments prepared in examples 1 to 5 and seed treatments in comparative examples 1 to 4 were subjected to seed soaking.
The tests were carried out in 3 and 4 months of 2020 in the key laboratory of ecological agriculture and biotechnology in the Hongze lake of Jiangsu province of the university of Huaiyin, and the peanut variety was white sand 1016, produced by Sanshen seed Limited in Qingdao, and purchased commercially.
Pretreating peanuts: soaking peanut seeds in 1.0% sodium hypochlorite solution for 20min for sterilization, and then washing with clear water for 2-3 times for later use.
Soaking seeds of the pretreated peanuts: the pretreated seeds were divided into 10 treatment groups, 9 of which were respectively applied with the seed treatment agents of examples 1 to 5 and comparative examples 1 to 4, 1 treatment group was soaked with clear water instead of the seed treatment agent as a blank control group, and the seed treatment agents of comparative examples 1 to 4 were used to directly soak the pretreated peanuts as a control group. The seed treatment agents prepared in examples 1 to 5 were diluted 100 times before seed soaking, and then used for pretreatment of peanut seed soaking, as test groups. 3 replicates were run for each treatment group.
The seed soaking conditions of the test group, the control group and the blank control group are completely consistent, and the seed soaking time is 16h.
Application example 1
After seed soaking is completed, seed germination and seedling quality are detected. Seeds are evenly sown in plastic pots paved with water absorbing paper, and 50 seeds are planted in each plastic pot. The wet gauze is used for covering and moisturizing all the time during the culture period, and the watering quantity can be reduced properly after the seeds germinate. The germination number of the seeds is recorded in the 5 th d, the germination vigor is calculated, the germination number of the seeds is recorded in the 7 th d, and the germination rate is calculated. The effect of different treatments on peanut seed germination was obtained and the test results are shown in table 1. And 12d, respectively measuring the fresh weight and the dry weight of the overground part of the seedling, the fresh weight and the dry weight of the root system, and simultaneously measuring the activity of the root system by adopting a TTC method. The effect of different treatments on the quality of peanut seedlings was obtained and the test results are shown in Table 2.
TABLE 1 Effect of different treatments on peanut seed germination
As is clear from Table 1, the seed germination effect is best after soaking peanuts in the seed treatment agent of the present invention. The average germination vigor of the seeds of the test group is increased by 8.62 percent compared with the CK group, and the average germination rate of the seeds is increased by 11.25 percent compared with the CK group, which are all obviously superior to the control treatment group.
TABLE 2 effects of different treatments on peanut seedling quality
As is clear from Table 2, after the seed treatment agent of the present invention was used to seed peanuts, the fresh weight of the aerial parts of the peanut seedlings after the treatment agent was increased by 9.45% as compared with CK, and the dry weight was increased by 20.25% as compared with CK. The fresh weight of the root system is increased by 54.55% compared with CK, and the dry weight is increased by 66.67% compared with CK. The root system activity was increased by 44.44% over CK. All significantly higher than the control treatment group.
In conclusion, the seed treating agent provided by the invention has the function of improving the seed activity as the peanut seed treating agent, can promote the germination of seeds, strengthen the quality of germinated seedlings, and lays a foundation for high and stable peanut yield.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (7)
1. The seed treatment agent is characterized by comprising the following components in parts by mass per liter:
35-40 g of calcium nitrate, 2-4 g of magnesium sulfate, 1-2 g of leucine and 1-2 g of glycine;
the seed treatment agent takes water as a dispersion medium.
2. The method for preparing a seed treatment of claim 1, comprising the steps of:
mixing calcium nitrate with water to obtain a calcium nitrate solution;
mixing the calcium nitrate solution with leucine, and standing to obtain an initial mixed solution;
mixing the initial mixed solution with magnesium sulfate to obtain a second mixed solution;
and mixing the second mixed solution with glycine to obtain the seed treating agent.
3. The method according to claim 2, wherein the time for the standing is 20 to 26 hours.
4. The method of claim 2, wherein the second mixed solution is mixed with glycine and then subjected to volume fixation to obtain the seed treatment agent.
5. Use of a seed treatment according to claim 1 or prepared by a method according to any one of claims 2 to 4 in seed soaking of peanuts.
6. A seed treatment agent according to claim 1 or a seed treatment agent prepared by the method of any one of claims 2 to 4, characterized in that the seed treatment agent is diluted 100 times and seed soaking is performed.
7. The method of claim 6, wherein the seed treatment is applied for a period of 12 to 18 hours.
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