Antibacterial fertilizer of sugarcane and preparation method thereof
Technical field
The invention belongs to field of agricultural production technologies, and in particular to a kind of antibacterial fertilizer of sugarcane and preparation method thereof.
Background technology
Sugarcane tends to have fungal disease, including the corruption of smut, red rot, pineapple disease, the tip from growth period to harvest time
Disease, it has a strong impact on the perennial root performance of sugarcane, and yield to China's cane stalk and the sugar content of cane stalk cause it is great
Influence.Currently for this problem, the prophylactico-therapeutic measures to sugarcane fungal disease is mainly chemical prevention, usually from chemistry
Medicament is to cane seedling seed soaking or sprays chemistry at the initial stage of a disease.But chemical agent etc. is soaked seed and sprayed to chemical agent
The problem of big chemical agent less effective, toxicity, difficult degradation, being compatible with the environment property difference be present in mode.Therefore, it is necessary to provide
A kind of significant effect, low toxicity, antibacterial fertilizer degradable, being compatible with the environment property is good, it is true to suppressing sugarcane in agricultural production to meet
The needs of fungus diseases.
The content of the invention
Based on this, in view of the above-mentioned problems, it is an object of the invention to provide a kind of significant effect, low toxicity, it is degradable, with
The bacteria inhibiting composition of the good promotion sugarcane production of Environmental compatibility.
Concrete technical scheme is as follows:
A kind of antibacterial fertilizer of sugarcane, is counted in parts by weight, including following components:Urea 5-20 parts;30% urea ammonium nitrate solution
1-20 parts;Dipotassium hydrogen phosphate 10-45 parts;Surfactant 0.1-10 parts;Biological bactericide 0.1-10 parts;Humate 2-15
Part;Bleeding agent 0.2-2.8 parts;Cosolvent 1-10 parts;Water 30-70 parts.
Wherein, described in component nitrogenous 30% urea ammonium nitrate solution, for example, when used urea ammonium nitrate solution contains
When the mass percentage concentration of nitrogen is 30%, its composition weight number in the antibacterial fertilizer of sugarcane is 1-20 parts, nitrogenous 30% urea
Ammonium nitrate solution includes by mass percentage for it, 44% ammonium nitrate, 34% urea and 22% water;When used
When the nitrogenous mass percentage concentration of urea ammonium nitrate solution changes, urea ammonium nitrate solution composition weight point in the antibacterial fertilizer of sugarcane
The corresponding adjustment of number.
In wherein some embodiments, the above-mentioned antibacterial fertilizer of sugarcane, including following components:Urea 8-13 parts;Nitrogenous 30%
Urea ammonium nitrate solution 5-11 parts;Dipotassium hydrogen phosphate 12-22 parts;Biological bactericide 0.1-4 parts;Surfactant 1-6 parts;Humic
Hydrochlorate 2-14;Bleeding agent 0.2-1 parts;Cosolvent 1-4 parts;Water 40-65 parts.Wherein, when the nitrogenous quality hundred of urea ammonium nitrate solution
When point concentration is 30%, its composition weight number in the antibacterial fertilizer of sugarcane is 5-11 parts.
In wherein some embodiments, in the above-mentioned antibacterial fertilizer of sugarcane, the biological bactericide includes shenqinmycin, middle life
One or more in rhzomorph, trichodermin;The surfactant includes rhamnolipid;The humate includes humic acid
One or more in potassium, sodium humate, molasses alcohol waste liquid.
In wherein some embodiments, biological bactericide is shenqinmycin, and surfactant is rhamnolipid, humic acid
Salt is potassium humate.
In wherein some embodiments, shenqinmycin, rhamnolipid, the mass ratio of potassium humate are:(1~2):(1~
5):6.
In wherein some embodiments, in the antibacterial fertilizer of above-mentioned sugarcane, the bleeding agent is APG or Tween 80, institute
It is glycerine to state cosolvent.
In wherein some embodiments, the above-mentioned antibacterial fertilizer of sugarcane also includes:
EDTA-Zn 0.1-1 parts;
The Boratex 0.1-1 parts of four water eight.
Present invention also offers the antibacterial fertile preparation method of above-mentioned sugarcane.
Concrete technical scheme is as follows:
The above-mentioned antibacterial fertile preparation method of sugarcane, comprises the following steps:
(1) humate is dissolved in water, obtains solution A;
(2) surfactant is added to the water, heating stirring, then adds nitrogenous 30% urea ammonium nitrate solution thereto,
Obtain solution B;
(3) above-mentioned solution A is mixed with solution B, obtains solution C;
(4) urea, dipotassium hydrogen phosphate are added in solution C, obtains solution D;
(5) after biological bactericide being dissolved in into cosolvent, add in solution D, and add bleeding agent thereto, stir
Produce.
In wherein some embodiments, in the above-mentioned antibacterial fertile preparation method of sugarcane, in step (1) described solution A, also
Dissolved with EDTA-Zn and the Boratex of four water eight.
In wherein some embodiments, in the above-mentioned antibacterial fertile preparation method of sugarcane, the heating stirring of step (2) includes:
At 50-80 DEG C, 10-20min is stirred.
Antibacterial fertilizer of sugarcane of the present invention and preparation method thereof has the advantages that:
Sugarcane tends to have fungal disease, including the corruption of smut, red rot, pineapple disease, the tip from growth period to harvest time
Disease, it has a strong impact on the perennial root performance of sugarcane, and yield to China's cane stalk and the sugar content of cane stalk cause it is great
Influence.Above-mentioned antibacterial fertilizer provided by the invention can significantly prevent, reduce the generation of sugarcane crop fungal disease, make being used in
During thing, foliage-spray, pouring root or the scheme of seed soaking can be selected, it is easy to use, it is easily operated.
The antibacterial fertilizer of sugarcane of the present invention, wherein biological bactericide can effectively suppress fungi and grow, surfactant,
The protein of phage surface is destroyed, while after adding humate, biological bactericide, surfactant and humate three
Play and cooperate with antibacterial effect, and with other components together, optimum organization, good preventing and treating is played to sugarcane fungal disease, and
Promote the effect of sugarcane production.
Further, the antibacterial fertilizer of sugarcane of the present invention, EDTA-Zn and the Boratex of four water eight is added, adds antibacterial fertilizer
In nutrient, sugarcane production can be advantageously promoted by having reached, and can enhancing sugarcane disease resistance effect, so as to carry
High sugarcane yield.The antibacterial fertilizer of sugarcane of the present invention, after suitable cosolvent, bleeding agent is added, have preferably water-soluble
With permeability.Meanwhile the above-mentioned antibacterial fertilizer of sugarcane, using environmentally friendly component, have fungistatic effect notable, low toxicity, easily drop
Solve, the advantages of being compatible with the environment property is good.
The antibacterial fertile preparation method of sugarcane of the present invention, step is simple, technique is simple and easy to control, is adapted to industrialized production.
Brief description of the drawings
Fig. 1 is containing ustilago scitaminea bacteria loop diameter block diagram in the antibacterial fertile culture medium of sugarcane;
Fig. 2 is the antibacterial fertilizer of sugarcane to ustilago scitaminea bacteria inhibition of germination statistical chart.
Embodiment
Bacteria inhibiting composition and its application below in conjunction with promotion sugarcane production of the specific embodiment to the present invention carry out more detailed
Thin explanation.
Embodiment 1
The antibacterial fertilizer of liquid sugarcane described in the present embodiment, it is counted in parts by weight, including following components:
Wherein, nitrogenous 30% urea ammonium nitrate solution is included by mass percentage, 44% ammonium nitrate, 34% urine
Element and 22% water, urea ammonium nitrate solution composition weight number in the antibacterial fertilizer of sugarcane is 12 parts.
Its preparation method comprises the following steps:
(1) each component is weighed by the parts by weight proportioning of said components;
(2) EDTA-Zn, the Boratex of four water eight, potassium humate are added to the water successively, it is stirring while adding, until completely
Dissolving, obtains solution A;
(3) by rhamnolipid add 60 DEG C of water in, stir 15min, then add thereto nitrogenous 30% urea ammonium nitrate it is molten
Liquid, stir, obtain solution B;
(4) above-mentioned solution A will enter into solution B, stirring while adding, produce solution C;
(5) by urea add solution C in, it is stirring while adding, until completely dissolved, addition can dipotassium hydrogen phosphate, side edged
Stirring, until being completely dissolved, obtain solution D;
(6) shenqinmycin is added in glycerine, it is stirring while adding, added after it is completely dissolved into solution D, stirring
To after well mixed, APG is added thereto, stirs and produces.
Embodiment 2
The antibacterial fertilizer of liquid sugarcane described in the present embodiment, it is counted in parts by weight, including following components:
Its preparation method is the same as embodiment 1.
Embodiment 3
The antibacterial fertilizer of liquid sugarcane described in the present embodiment, it is counted in parts by weight, including following components:
Its preparation method is the same as embodiment 1.
Embodiment 4
The antibacterial fertilizer of liquid sugarcane described in the present embodiment, it is counted in parts by weight, including following components:
Its preparation method is the same as embodiment 1.
Comparative example 1
This comparative example provides a kind of antibacterial fertilizer of sugarcane, and it reduces rhamnolipid, its preparation method relative to embodiment 1
With embodiment 1.
Comparative example 2
The present embodiment provides a kind of antibacterial fertilizer of sugarcane, reduces humate relative to embodiment 1, its preparation method is same
Embodiment 1.
The smut of sugarcane mycelia growth inhibition test of experimental example 1
PDA culture medium is prepared with the antibacterial fertilizer of the sugarcane described in same amount of 1-4 of the embodiment of the present invention and comparative example 1, will be sweet
Sugarcane black tassel bacteria is seeded to culture medium, in 28 DEG C, after cultivating 10 days, determines bacterium loop diameter.As a result as shown in Figure 1 and Figure 2, contrast
A diameter of 37.21cm of ustilago scitaminea bacteria circle in example 1, comparative example 2 are 37.53cm, and embodiment 1 is 11.54cm, embodiment 2
For 11.61cm, embodiment 3 is 12.42cm, and embodiment 4 is 14.65cm.
Understand by contrast, the antibacterial fertilizer in the embodiment of the present invention is substantially excellent to the growth inhibitory effect of ustilago scitaminea bacteria
In comparative example, the effect of wherein embodiment 1 is best, and other embodiment effect is still significantly better than and has lacked surfactant
Comparative example 1 and the comparative example 2 for having lacked humate.
Influence experiment of the antibacterial fertilizer of the sugarcane of experimental example 2 to ustilago scitaminea bacteria spore germination
After the antibacterial nutriment in a fertilizer of sugarcane described in same amount of 1-4 of the embodiment of the present invention and comparative example 1-2 is not diluted into 250 times,
It is 10 wherein to add smut of sugarcane teleutospore compound concentration6Individual/ml spore suspension, draw 5mL spore suspensions in 10mL from
In heart pipe, in constant-temperature table, 28 DEG C, 130r/min, after cultivating 24 hours, the antibacterial fertilizer of measure sugarcane is to ustilago scitaminea bacteria spore
The influence that son is sprouted, using with the concentration that sterilized water is prepared as 106Individual/ml spore suspension makees blank control.As a result such as Fig. 2 institutes
Show, the spore germination rate of blank control group is 94%, and the spore germination rate of comparative example 1 is 88.6%, and the spore of comparative example 2 is sprouted
Hair rate is 67.4%, and the spore germination rate of embodiment 1 is 51.67%, and embodiment 2 is 53.5%, and embodiment 3 is 53%, is implemented
Example 4 is 60.17%.
According to spore germination rate, inhibiting rate of the antibacterial fertilizer of sugarcane to ustilago scitaminea bacteria spore germination can be calculated, such as table 1
It is shown.
Sample |
Inhibition of germination (%) |
Embodiment 1 |
45.03 |
Embodiment 2 |
43.09 |
Embodiment 3 |
43.61 |
Embodiment 4 |
35.98 |
Comparative example 1 |
5.67 |
Comparative example 2 |
28.29 |
Table 1
Understand by contrast, the sugarcane after antibacterial fertile (including surface active agent composition) processing of sugarcane described in embodiment 1-4
Black tassel bacteria spore germination rate is significantly inhibited, and sugarcane described in comparative example 1 is antibacterial fertile (not including surface active agent composition)
And the effect that sugarcane described in comparative example 2 antibacterial fertile (not including humate composition) suppresses to ustilago scitaminea bacteria spore germination
It is poor.Therefore, the antibacterial fertilizer of sugarcane of the present invention has to ustilago scitaminea bacteria spore germination significantly inhibits effect.
1-4 of the embodiment of the present invention, component include shenqinmycin, rhamnolipid and humate, and three plays collaboration suppression
The effect of bacterium, the ratio between three, i.e. shenqinmycin:Rhamnolipid:Humate is (1~2):(1~5):It is when 6, i.e., real
It is better to apply a 1-3.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the present invention's
Protection domain.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.