CN109425700B - Method for testing relation between populus diversifolia seed germination capacity and medium salt content - Google Patents
Method for testing relation between populus diversifolia seed germination capacity and medium salt content Download PDFInfo
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Abstract
The invention relates to a method for testing the relation between the germination capacity of populus diversifolia seeds and the salt content of a matrix, which comprises the steps of constructing a populus diversifolia seed cultivation model; acquiring germination capacity data; acquiring data of the salt content of the matrix; and constructing a relation curve according to the growth rate of the populus diversifolia seeds in the germination period and the salt content data of the matrix. According to the method for testing the relation between the germination capacity of the populus diversifolia seeds and the content of the matrix salt, the growth rate of the populus diversifolia seeds in a germination period is calculated by collecting the time three-dimensional model diagram of the populus diversifolia seeds, so that the growth rate of the populus diversifolia seeds can be accurately measured, and the error of the relation curve between the growth rate of the populus diversifolia seeds in the germination period and the content data of the matrix salt is reduced; in addition, when the growth rate of the populus diversifolia seeds in the germination period is obtained through calculation, a vertex coordinate calculation mode in the XYZ three-axis direction and a projection area calculation mode on the XYZ three surfaces are used, and the final error can be reduced to the maximum extent.
Description
Technical Field
The invention relates to the field of populus diversifolia seed testing, in particular to a method for testing the relation between the germination capacity of populus diversifolia seeds and the salt content of a matrix.
Background
Populus diversifolia is distributed in desert and semi-desert areas, and the ecological environment is extremely harsh. Populus euphratica can survive under extremely harsh ecological environment conditions, can become an ancient and long-lived rare tree species, and has close relation with the unique biological and ecological characteristics of the popus euphratica.
Populus diversifolia leaves are changeable in shape and curious. When you walk into a poplar forest, you see first that its leaf shapes are different. On the bud of the base of the young seedling or the adult seedling, the leaves are in a shape of a cape of needles, and are long and narrow like willows; while the shape of the leaves on adult branches is quite different, such as oval, diamond, heart, semi-circle, and triangle. The phenomenon that different shapes of leaves grow out at different development stages is the most prominent biological characteristic of populus euphratica. This heterophyllic phenomenon is known botanically as "evolutionary heterophyllic". It is because of the varying leaf morphology of populus euphratica, which is also commonly referred to as "iso-poplar". It is also believed that populus diversifolia has five leaves with different shapes and sizes on one tree, thus it is called "five-in-one tree" in a game. People feel rarer and exclamatory whenever a poplar tree with various leaf shapes is seen. In fact, the leather of the special-shaped leaves and leaves of populus euphratica is beneficial to reducing the evaporation and consumption of salt in vivo, and is adaptive to drought environment.
Populus diversifolia is not a drought-resistant tree species, but has strong drought resistance. Plants growing in arid desert regions, which cannot survive on atmospheric precipitation, require a source of salt for their growth, mainly groundwater and runoff water from rivers and soil water obtained by condensation from the air. Therefore, desert plants must have large horizontal root systems and strong root osmotic pressures. Meanwhile, the overground parts of the plants also need to have a drought-growing morphological structure so as to reduce salt evaporation and adjust the salt balance in the body. Populus diversifolia can survive for five months after long-term water accumulation in the lake basin because of being not afraid of water bubbles. The populus euphratica can not be frozen to death after accumulated water climbs ice for four or five months in winter. If the populus euphratica grows in the clay layer, the moisture content of the soil is more than 20%, and the roots of the populus euphratica cannot rot. This shows that populus diversifolia not only has strong water resistance, but also has strong corrosion resistance, especially after soaking in water.
The populus euphratica fruits have long maturing period and are required to be durable for picking the fruits. Populus diversifolia is a male and female alien plant, flowers are firstly planted, then leaf trees are planted, and when a single plant grows to 12 years old, the populus diversifolia can bloom and bear fruits. Generally, populus diversifolia blooms from late 4 months to early 5 months, and the flowering period is about 15 days; the mature period of the fruit begins in late 6 months and can be prolonged to late 8 months, and the mature period of the fruit from flowering to flowering is more than 120 days, and is the tree species with the longest mature period of the fruit in the family Salicaceae. When the mature period of populus euphratica fruits comes in seven or eight months every year, a large number of seeds fly with white crown hairs in a wandering manner for hundreds of kilometers while flying by wind, and the populus euphratica exactly looks like snowfall in midsummer and becomes a large landscape in the nature. Although the populus euphratica is old and long in life, the populus euphratica has very small seeds, the weight of each thousand seeds is only 0.08-0.2 g, and the number of each gram of seeds is as many as 12000, which is quite rare. Populus diversifolia seeds are small, and the germination capacity can only be kept for 30 days under natural conditions. Therefore, the problem that the poplar seeds have the strongest germination capacity under the condition of what salinity is urgently needed to be solved currently.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a method for testing the relation between the germination capacity of populus diversifolia seeds and the salt content of a matrix, which can accurately and unmistakably measure the relation between the populus diversifolia seeds and the salt content of the matrix and eliminate the influence of factors such as salt, trace elements, temperature, humidity, illumination and the like on the testing process.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
a method for testing the relation between the germination capacity of populus diversifolia seeds and the salt content of a matrix comprises the following steps:
populus diversifolia seed treatment: soaking populus diversifolia seeds in a plant nutrient solution for 10min-120min, wherein the plant nutrient solution comprises a populus diversifolia seedling extracting solution; ultrasonic treatment is carried out simultaneously in the soaking process, the power of the ultrasonic is 100-200W, each time of ultrasonic treatment is 5-8s, and the interval between two adjacent ultrasonic treatments is 20-30 s;
constructing a populus diversifolia seed cultivation model: the method comprises the following steps of arranging populus diversifolia seeds in a circular shape in a culture medium, and adding a soil conditioner into the culture medium, wherein the soil conditioner comprises the following raw materials in parts by weight:
100-150 parts of diatomite, 80-120 parts of fly ash, 70-90 parts of bentonite, 50-70 parts of kaolin, 80-120 parts of turf, 120-160 parts of plant straw powder, 12-20 parts of algae-containing carriers, 40-60 parts of organic fertilizer, 40-70 parts of humus, 20-35 parts of zeolite powder, 20-30 parts of glass beads, 18-25 parts of silica powder, 3-5 parts of hydrophobic resin and 20-30 parts of microbial liquid;
10-20 parts of polypropylene glycol, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of traditional Chinese medicine waste residue powder, 20-40 parts of waste tea residues, 8-24 parts of fulvic acid concentrated solution, 40-60 parts of vinasse, 30-60 parts of leaf mold, 40-60 parts of sawdust, 20-30 parts of rice chaff ash, 10-30 parts of phosphorus, 50-70 parts of coconut husk and 30-40 parts of slow release fertilizer;
acquiring germination capacity data: in a populus diversifolia seed germination period, acquiring a time three-dimensional model diagram of a populus diversifolia seed cultivation model to be detected through a three-dimensional scanner; the time three-dimensional model map comprises three-dimensional model maps corresponding to a plurality of populus diversifolia seed cultivation models to be detected at different moments; analyzing and processing through a time three-dimensional model diagram, and further calculating to obtain the growth rate of the populus euphratica seeds in the populus euphratica seed cultivation model in a germination period;
obtaining the salt content data of the matrix: collecting a culture medium sample around the populus euphratica seeds in the populus euphratica seed cultivation model to be detected in a populus euphratica seed germination period; measuring the content data of the salt in the culture matrix sample around the populus diversifolia seeds by using a salt tester, and calculating the content data of the salt in the culture matrix sample around the populus diversifolia seeds to obtain the content data of the salt in the matrix;
and constructing a relation curve between the growth rate of populus euphratica seeds in a germination period and the salt content of the substrate.
The inventor of the patent finds that the reproductive capacity of the populus diversifolia seeds becomes stronger when the populus diversifolia seeds are soaked in the plant nutrient solution, so that the survival rate of the populus diversifolia seeds is improved, and the error of a relation curve between the growth rate of the populus diversifolia seeds in a germination period and the salt content of a matrix is reduced.
Secondly, adding a poplar seedling extracting solution into the plant nutrient solution to enable poplar seeds to absorb the poplar seedling juice in advance before germination, so that the survival rate of the poplar seeds can be improved; the volume percentage of the poplar seedling extracting solution in the culture solution is 5-30%. In addition, experiments show that the addition of the poplar seedling extracting solution with excessive content easily causes the contamination of the poplar seeds, so that the problem of good survival rate improvement is solved and the poplar seeds are not infected by adding the proper amount of the poplar seedling extracting solution.
In the soil conditioner provided by the invention, various components with specific compatibility are selected, and the soil conditioner can be specifically divided into a conditioning component and a sand fixing component. The sand fixing component mainly comprises polypropylene glycol, hydrophobic resin and bentonite, and is used for realizing the function of fixing loose soil (sand) to form a formed sand fixing layer. The components are mixed according to a predetermined compatibility relationship to form an environment which is very beneficial to the growth of the populus euphratica seeds; the environment is also very beneficial to the rapid growth and metabolism of microorganisms (the fulvic acid concentrated solution and humus have main effects); the fulvic acid concentrated solution and humus act together to improve the microenvironment of populus diversifolia seeds; promoting the transformation of substances and energy in the growing process of the populus euphratica seeds, so that the populus euphratica seeds show a healthy situation; meanwhile, the growth of harmful fungi can be inhibited, and nutrients required by growth of populus diversifolia seeds are supplied; has mild effect and is biodegradable. It should be particularly noted that, in the present application, the improved components composed of the algae-containing carrier, the glass beads, the silica powder, the microbial liquid, the fulvic acid concentrated solution, the waste tea leaves, the Chinese medicine waste residue powder, the rice chaff ash and the like are formulated with low creativity, and after the components are mixed and matched, the combination is unexpectedly found to have very significant low improvement on the water retention and air permeability of the matrix soil where the populus diversifolia seeds are located, and the survival rate of the populus diversifolia seeds is improved.
In addition, the plant straw powder comprises the following components in percentage by weight:
8-20% of corn stalk powder, 6-20% of corn cob powder, 9-25% of potato straw powder, 10-30% of sea buckthorn straw powder, 10-15% of camel thorn straw powder, 10-15% of rice straw powder, 8-15% of flax straw powder, 5-15% of wheat straw powder, 5-20% of sorghum straw powder and 5-20% of pea straw powder.
The microbial liquid is a liquid obtained by culturing and fermenting one or more of actinomycetes, azotobacter, bacillus subtilis, photosynthetic bacteria and saccharomycetes.
After the compound microbial bacteria liquid, the organic biofertilizer and the humic acid act, a series of metabolites can be generated, and the purposes of preventing diseases and resisting insects are achieved through competition on space and nutrition and generation of various antibacterial and insect-resistant substances. In addition, the growth and development of the populus euphratica seeds can be stimulated, the stress resistance of crops is improved, and the growth and development of the populus euphratica seeds are promoted.
The vermiculite powder has good cation exchange property, adsorbability and water retention property, improves the microenvironment of the root surface, and stores water and is breathable; can buffer the pH value of the environment around the root system, keep the nutrient slowly released, provide mineral elements and promote the growth of the populus euphratica seeds.
The fixing agent has the function of film formation, so that other components can be smoothly coated on the surface of the populus euphratica seeds. The soil conditioner prepared by mixing the components according to the weight ratio has the advantages that the components exert respective effects, and meanwhile, the different components generate synergistic action, so that the coating agent generates related components such as bacteriostasis, insect prevention, organic and inorganic nutrients, growth promoting factors and the like, the germination of populus diversifolia seeds can be promoted, and the survival rate of the populus diversifolia seeds is improved. More importantly, all components of the coating agent are all natural components, can be biologically degraded, are harmless to people, livestock and environment, and meet the requirement of agricultural production without public nuisance.
The algae-containing carrier is loaded with two or more than two high molecular polymer materials of Coccomyxa, Pseudocochliobolus, Lepisorus, Pseudocladosporium, Sphingomonas, Anabaena, Coccomyxococcus and Nostoc.
In view of the fact that Coccomyxa, Pseudocococcus, Leptomyza, Pseudocladosporium, Sphingomonas and Microcoleus have strong reproductive capacity and stress resistance, most of the algae are in lichen symbiosis, and the combination of the algae can show synergistic effect. Therefore, it is preferable to use several kinds of algae as the breeding species. In addition, the algae-containing carrier is preferably a high molecular polymer material having a good water retention capacity and being easy to flow, and is intended to improve the survival ability and the propagation performance of algae.
Preferably, the soil conditioner is prepared by the following steps:
1) mixing kaolin and fly ash uniformly, adding water, stirring into a dough, and heating the dough for 1-1.5 hours at the temperature of 30-35 ℃ to obtain a first mixture;
2) uniformly mixing diatomite and turf, adding the fulvic acid concentrated solution, adding water, and continuously mixing to obtain a second mixture;
3) adding a straw decomposition agent into plant straw powder, decomposing the plant straw powder, mixing the plant straw powder with an organic fertilizer, humus, traditional Chinese medicine waste residue powder and waste tea residues, stacking and fermenting the mixture, mixing the mixture with vinasse, leaf rotting soil, sawdust, rice chaff ash and loose phosphorus mixed coconut coir, uniformly stirring the mixture, and standing the mixture for 2 to 3 days at the temperature of between 20 and 30 ℃ to obtain a third mixture;
4) mixing the zeolite powder, the glass beads and the silica powder, adding the hydrophobic resin, and uniformly stirring; obtaining a fourth mixture;
5) uniformly mixing the second mixture and the third mixture, adding urea, monoammonium phosphate and a slow release fertilizer, dispersing and stirring, adding the first mixture and the fourth mixture, uniformly stirring, adding an algae-containing carrier and a microbial liquid, continuously uniformly mixing, and spreading to the environment of 25-30 ℃ for 2-5 hours to obtain a pre-used powder material;
6) adding water with the mass 2-4 times of that of the bentonite and inorganic salt with the mass 1-4% of that of the bentonite into the bentonite, stirring or extruding for modification, and then carrying out classification and purification to obtain slurry with the particle size of more than 90% and less than 10 mu m; and mixing the slurry with a water solution of polypropylene glycol dissolved in water and water, uniformly stirring at a rotating speed of 20-30 r/min, and separately packaging to obtain a pre-used liquid material.
In addition, through ultrasonic treatment, the soaking time of the populus diversifolia seeds can be shortened, and the survival rate of the populus diversifolia seeds is further improved.
The poplar seedling extracting solution is prepared by the following steps:
heating the populus diversifolia seedlings and water with the mass 5-10 times that of the populus diversifolia seedlings at high pressure for 1-2 hours, and filtering to obtain filtrate and filter residues;
putting the filter residue and the filtrate into a closed container, and inoculating zymophyte into the container for fermentation for 4-6 h;
and filtering to obtain stock extract after fermentation.
Experiments show that the stock extracting solution obtained by fermenting the filtrate and the filter residue can further improve the survival rate of the grafted nursery stock.
Preferably, in the populus diversifolia seed cultivation model, the distance between adjacent populus diversifolia seeds is not more than 15cm, and the number of the populus diversifolia seeds in the culture medium is not more than 40.
In the process of acquiring the germination capacity data, abnormal data of the germination capacity of the populus euphratica seeds are removed, the removed abnormal data are mainly populus euphratica seeds with abnormal germination or germination, and the germination rate of the populus euphratica seeds is very low or deviates from a normal range.
Preferably, the analysis and processing are carried out through a time three-dimensional model map, and then the growth rate of the populus euphratica seeds in the populus euphratica seed cultivation model in the germination period is calculated and obtained, and the method comprises the following steps:
acquiring a three-dimensional coordinate of a time three-dimensional model diagram to obtain a vertex coordinate in an XYZ three-axis direction;
and (3) calculating the growth rate of the populus diversifolia seeds in the germination period according to the vertex coordinates:
preferably, the analysis and processing are carried out through a time three-dimensional model map, and then the growth rate of the populus euphratica seeds in the populus euphratica seed cultivation model in the germination period is calculated and obtained, and the method comprises the following steps:
acquiring a three-dimensional coordinate of a time three-dimensional model diagram, and further acquiring a projection area of the three-dimensional model diagram in the direction of XYZ three axes;
calculating the growth rate of the populus diversifolia seeds in the germination period according to the projection area in the direction of three XYZ axes:
preferably, the collection positions of the culture medium samples are the upper end, the lower end, the left end, the right end, the front end and the rear end of the central coordinate of the populus diversifolia seed.
Preferably, the matrix salt content data is calculated by the following formula:
Ct＝Kon the upper part*Wt On the upper part+KLower part*Wt Lower part+KLeft side of*Wt Left side of+KRight side*Wt Right side+KFront side*Wt Front side+KRear end*Wt Rear end。
Preferably, after constructing a salt calibration model based on the growth rate of populus diversifolia seeds during the germination cycle and the matrix salt content data:
collecting culture medium samples of the upper end, the lower end, the left end, the right end, the front end and the rear end of central coordinates of adjacent seeds of the populus diversifolia seeds in a germination period of the populus diversifolia seeds, measuring salt content data in the culture medium samples of the adjacent seeds by a salt tester, and then calculating to obtain a salt correction coefficient;
and correcting a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content of the matrix according to the salt correction coefficient.
Preferably, the calculation formula of the matrix salt content data is as follows:
preferably, the measurement of germination capacity data is performed on a model built in 3-matic forward design software.
Preferably, a temperature correction model and a moisture correction model are constructed after a relation curve is constructed according to the growth rate of the populus diversifolia seeds in the germination period and the matrix salt content data, and a temperature correction coefficient and a moisture correction coefficient are obtained by the temperature correction model and the moisture correction model;
and correcting a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content of the matrix according to the temperature correction coefficient and the moisture correction coefficient.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the method for testing the relation between the germination capacity of the populus diversifolia seeds and the salt content of the matrix, provided by the invention, the round populus diversifolia seed cultivation model is adopted, the construction of multiple sets of models can be guaranteed in one culture medium, the same cultivation environment can be guaranteed as much as possible in the same culture medium, and the error of a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content data of the matrix is reduced.
2. According to the method for testing the relation between the germination capacity of the populus diversifolia seeds and the salt content of the matrix, the growth rate of the populus diversifolia seeds in a germination period is calculated by collecting the time three-dimensional model diagram of the populus diversifolia seeds, so that the growth rate of the populus diversifolia seeds can be accurately measured, and the error of the relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content of the matrix is reduced; in addition, when the growth rate of the populus diversifolia seeds in the germination period is obtained through calculation, a vertex coordinate calculation mode in the XYZ three-axis direction and a projection area calculation mode in the XYZ three-axis direction are used, and the final error can be reduced to the maximum extent.
3. According to the method for testing the relation between the germination capacity of the populus diversifolia seeds and the matrix salt content, the matrix salt content is finally calculated by collecting the culture matrix samples around the populus diversifolia seeds in the to-be-tested populus diversifolia seed cultivation model, particularly the collection positions of the culture matrix samples are the upper end, the lower end, the left end, the right end, the front end and the rear end of the central coordinates of the populus diversifolia seeds, and the error of the relation curve between the growth rate of the populus diversifolia seeds in the germination period and the matrix salt content can be reduced.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The embodiment of the invention provides a method for testing the relation between the germination capacity of populus diversifolia seeds and the salt content of a matrix, which comprises the following steps:
populus diversifolia seed treatment: soaking populus diversifolia seeds in a plant nutrient solution for 10min-120min, wherein the plant nutrient solution comprises a populus diversifolia seedling extracting solution; ultrasonic treatment is carried out simultaneously in the soaking process, the power of the ultrasonic is 100-200W, each time of ultrasonic treatment is 5-8s, and the interval between two adjacent ultrasonic treatments is 20-30 s;
constructing a populus diversifolia seed cultivation model: the method comprises the following steps of arranging populus diversifolia seeds in a circular shape in a culture medium, and adding a soil conditioner into the culture medium, wherein the soil conditioner comprises the following raw materials in parts by weight:
100-150 parts of diatomite, 80-120 parts of fly ash, 70-90 parts of bentonite, 50-70 parts of kaolin, 80-120 parts of turf, 120-160 parts of plant straw powder, 12-20 parts of algae-containing carriers, 40-60 parts of organic fertilizer, 40-70 parts of humus, 20-35 parts of zeolite powder, 20-30 parts of glass beads, 18-25 parts of silica powder, 3-5 parts of hydrophobic resin and 20-30 parts of microbial liquid;
10-20 parts of polypropylene glycol, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of traditional Chinese medicine waste residue powder, 20-40 parts of waste tea residues, 8-24 parts of fulvic acid concentrated solution, 40-60 parts of vinasse, 30-60 parts of leaf mold, 40-60 parts of sawdust, 20-30 parts of rice chaff ash, 10-30 parts of phosphorus, 50-70 parts of coconut husk and 30-40 parts of slow release fertilizer;
constructing a populus diversifolia seed cultivation model: arranging populus diversifolia seeds in a culture medium according to a circle; the circular seed arrangement mode is adopted, so that the mutual influence among the cultivation models is reduced to the minimum, and meanwhile, the mutual influence among the cultivation models is relatively even; in the process of constructing the breeding model of the populus euphratica seeds, the distance between adjacent populus euphratica seeds is generally not more than 15cm, and the number of the populus euphratica seeds in the culture medium is not more than 40; firstly, a circular populus diversifolia seed cultivation model is adopted, so that the construction of a plurality of models in one culture medium can be guaranteed; and secondly, the same culture environment can be ensured as much as possible in the same culture medium, and the error of a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salinity content data of the matrix is reduced. Of course, in the construction of the populus diversifolia seed training model, other regular-shaped arrangement modes can be adopted, other irregular-shaped arrangement modes can be adopted, and the method for obtaining the relation between the germination capacity of the populus diversifolia seeds and the content of the substrate salt can still be obtained. However, a large number of repeated experiments show that the dispersion coefficient of a relation curve between the growth rate and the substrate salt content data measured by the poplar seed arrangement mode with other shapes (including regular shapes and irregular shapes) is large, the dispersion coefficient is close to 1 after only the circularly arranged poplar seeds are measured for multiple times, and the measurement result is accurate.
In the process of acquiring the germination capacity data, abnormal data of the germination capacity of the populus euphratica seeds are removed, the removed abnormal data are mainly populus euphratica seeds with abnormal germination or germination, and the germination rate of the populus euphratica seeds is very low or deviates from a normal range.
Acquiring germination capacity data, wherein the germination capacity is characterized by the growth rate of populus euphratica seeds in a germination period: in a populus diversifolia seed germination period, acquiring a time three-dimensional model diagram of a populus diversifolia seed cultivation model to be detected through a three-dimensional scanner; the time three-dimensional model map comprises three-dimensional model maps corresponding to a plurality of populus diversifolia seed cultivation models to be detected at different moments; analyzing and processing through a time three-dimensional model diagram, and further calculating to obtain the growth rate of the populus euphratica seeds in the populus euphratica seed cultivation model in a germination period;
in the present invention, there are two embodiments for calculating the growth rate, namely a three-dimensional distance difference method, which comprises the following steps: analyzing and processing through the time three-dimensional model diagram, and further calculating to obtain the growth rate of the populus euphratica seeds in the populus euphratica seed cultivation model in the germination period, wherein the growth rate comprises the following steps:
obtaining three-dimensional coordinates of a time three-dimensional model diagram, and obtaining vertex coordinates in XYZ three-axis directions at a certain moment and a next moment corresponding to the moment, namely XYZ three-axis directions at the next moment and the moment corresponding to the moment at the certain momentUpward corresponding extreme points respectively; suppose that at time t, the maximum point and the minimum point on the X-axis are (X) respectivelyt max，y1，z1)、(Xt min，y2，z2) (ii) a The maximum point and the minimum point on the Y axis are (x) respectively3，Yt max,，z3)、(x4，Yt min，z4) (ii) a The maximum point and the minimum point on the Z axis are respectively (x)5，y5，Zt max)、(x6，y6,,，Zt min) Suppose that at time t + △ t, the maximum point and the minimum point on the X-axis are (X)t+1 max，y1 *，z1 *)、(Xt+1 min，y2 *，z2 *) (ii) a The maximum point and the minimum point on the Y axis are (x) respectively3 *，Yt+1 max，z3 *)、(x4 *，Yt+1 min，z4 *) (ii) a The maximum point and the minimum point on the Z axis are respectively (x)5 *，y5 *，Zt+1 max)、(x6 *，y6 * ,,，Zt+1 min)
And calculating the growth rate of the populus diversifolia seeds in the germination period according to the vertex coordinates of the t moment and the t +1 moment:
in the formula 1, VtIs the growth rate of populus diversifolia seeds in a germination period;
λ1、λ2、λ3、λ4、λ5、λ6are all distance difference square coefficients, where1、λ2、λ4、λ5The value range of (A) is between 0.95 and 1.05, lambda3The value range of (A) is between 0.27 and 0.67, lambda6The value range of (1) is 1.84-3.89. Because the top end (in the Z-axis direction) of the populus diversifolia seeds usually has a high growth speed and the bottom end of the populus diversifolia seeds has a low growth speed relative to other directions in the growth process of the populus diversifolia seeds, in order to ensure that the growth rate of the populus diversifolia seeds in the germination period can be relatively accurately obtained, lambda is used3Has a relatively small value range of lambda6The value range of (a) is relatively large. And λ3/λ6Preferably with
In inverse proportion.
In the invention, one of other calculation modes of the growth rate is a projection area difference method, and the method comprises the following specific processes: analyzing and processing the time-lapse three-dimensional model diagram, and further calculating the growth rate of the populus euphratica seeds in the populus euphratica seed cultivation model in the germination period, wherein the growth rate comprises the following steps:
acquiring three-dimensional coordinates of a time three-dimensional model diagram, and further acquiring the projection area of the time three-dimensional model diagram in the XYZ three-axis directions at a certain moment and the next moment corresponding to the moment; suppose that at time t, the projection areas of the three-dimensional model map in the XYZ three-axis directions are St X、St Y、St ZAt time t + △ t, the projection areas of the three-dimensional model map in the three XYZ axes are St+1 X、St+1 Y、St+1 Z
Calculating the growth rate of the populus diversifolia seeds in the germination period according to the projection area in the direction of three XYZ axes:
in the formula 2, VtIs the growth rate of populus diversifolia seeds in a germination period; mu.s1、μ2、μ3Are all projection area difference square coefficients, where1、μ2The value range of (1) is between 0.72 and 0.86 mu3The value range of (a) is between 0.94 and 1.17. Because the projection area in the Z-axis direction is relatively small in change and the projection area in the X, Y-axis direction is relatively large in change in the growth process of the populus euphratica seeds, mu is used for ensuring that the growth rate of the populus euphratica seeds in the germination period can be relatively accurately obtained3Has a relatively large value range of mu1、μ2Has a relatively small value range of and mu1/μ2And (S)t+1 X-St X)/(St+1 Y-St Y) In inverse proportion.
In the invention, the measurement of the germination capacity data is carried out on a model established in 3-matic forward design software.
Obtaining the salt content data of the matrix: collecting a culture medium sample around the populus euphratica seeds in the populus euphratica seed cultivation model to be detected in a populus euphratica seed germination period; in order to accurately measure the data of the salinity content of the substrate and improve the data precision, the collection positions of the culture substrate samples are the upper end, the lower end, the left end, the right end, the front end and the rear end of the central coordinate of the populus diversifolia seeds; supposing that at the time t, the samples of the upper end, the lower end, the left end, the right end, the front end and the rear end of the central coordinate of the populus diversifolia seed are respectively MOn the upper part、MLower part、MLeft side of、MRight side、MFront side、MRear end。
Determination of culture substrate sample M by salinity testerOn the upper part、MLower part、MLeft side of、MRight side、MFront side、MRear endThe data of medium salt content are respectively WOn the upper part、WLower part、WLeft side of、WRight side、WFront side、WRear endThen, calculating the salt content data of the substrate through the salt content data in the culture substrate sample around the populus diversifolia seeds to obtain the salt content data of the substrate; the salt content of the matrix is calculated by the following formula：
Ct＝KOn the upper part*Wt On the upper part+KLower part*Wt Lower part+KLeft side of*Wt Left side of+KRight side*Wt Right side+KFront side*Wt Front side+KRear end*Wt Rear end(3)
In formula 3, CtThe salt content of the matrix; kOn the upper part、KLower part、KLeft side of、KRight side、KFront side、KRear endAll are salt content coefficients; wherein, KLeft side of、KRight side、KFront side、KRear endThe value range of (A) is between 0.87 and 1.12; kOn the upper partThe value range of (A) is between 0.24 and 0.32; kLower partThe value range of (A) is 1.37-1.87, wherein KOn the upper part、KLower partIt is preferable to satisfy:
in formula 4, HOn the upper partFor culturing the substrate sample MOn the upper partPerpendicular distance of collection position from substrate surface, HLower partFor culturing the substrate sample MLower partThe perpendicular distance of the collection location from the substrate surface. Because the reference value of the salt content of the substrate of the populus diversifolia seeds needs to integrate the salt data of each position on the periphery of the seeds in the germination period of the populus diversifolia seeds, samples of the upper end, the lower end, the left end, the right end, the front end and the rear end of the central coordinates of the populus diversifolia seeds are selected and have certain representativeness; in addition, the salt data at the upper end of the seeds has small influence on the growth rate of the seeds, and the salt data below the seeds has large influence on the growth rate of the seeds, so the method has the advantages of low cost, high yield and the like
So as to improve the accuracy of the salt content data of the matrix.
And constructing a relation curve according to the growth rate of populus diversifolia seeds in a germination period and the salt content data of the matrix.
In the present invention, a correction model can also be constructed, and the construction of the correction model includes many steps in general: a salinity correction model, a temperature correction model and a moisture correction model; the above correction models may be used alone or in combination.
Firstly, a salinity calibration model is introduced: because the model is cultivated to the chinese populus seed at the in-process of constructing, there are a plurality of chinese populus seeds to arrange in the culture medium, therefore when measuring certain chinese populus seed matrix salinity content data, the chinese populus seed adjacent with it also can absorb surrounding salinity, consequently can cause certain chinese populus seed matrix salinity content data to a certain extent and measure to have an error, consequently salinity correction adopts following mode to carry out:
collecting culture medium samples of upper end, lower end, left end, right end, front end and rear end of central coordinates of adjacent seeds of populus diversifolia seeds in a populus diversifolia seed germination period, measuring salt content data in the culture medium samples of the adjacent seeds by a salt content tester, and calculating according to a formula (3) to obtain the salt content C of the medium of the adjacent seedst 1、Ct 2Calculating a salinity correction coefficient η according to the salinity content of the substrate of the adjacent seeds, wherein the calculation formula of η is as follows:
multiplying C by salinity correction factortAnd obtaining the corrected matrix salt content, and then constructing a relation curve of the growth rate of the populus diversifolia seeds in the germination period and the corrected matrix salt content data.
Next, a temperature correction model is introduced: collecting temperature data of the environment where populus diversifolia seeds are located in a germination period to obtain a daily temperature change curve; obtaining an average temperature change curve according to the temperature change curve of each day; and obtaining a corresponding temperature correction coefficient according to the temperature change curve of each day and the average temperature change curve.
Aiming at the treatment steps of the populus diversifolia seeds and the steps of constructing a populus diversifolia seed cultivation model in the scheme in the patent, the following embodiments are set:
example 1
Soaking populus diversifolia seeds in a plant nutrient solution for 30min, wherein the plant nutrient solution comprises a populus diversifolia seedling extracting solution; ultrasonic treatment is carried out simultaneously in the soaking process, the power of ultrasonic is 150W, the ultrasonic treatment is carried out for 6s each time, and the interval between two adjacent ultrasonic treatments is 20 s;
the plant nutrient solution is a conventional commercial nutrient solution;
constructing a populus diversifolia seed cultivation model: 20 populus diversifolia seeds are arranged in a circular shape in a culture medium, a soil conditioner is added into the culture medium, and the soil conditioner comprises the following raw materials in parts by weight:
100 parts of diatomite, 80 parts of fly ash, 70 parts of bentonite, 50 parts of kaolin, 80 parts of turf, 120 parts of plant straw powder, 12 parts of an algae-containing carrier, 40 parts of an organic fertilizer, 40 parts of humus, 20 parts of zeolite powder, 20 parts of glass microspheres, 18 parts of silica powder, 3 parts of hydrophobic resin and 20 parts of microbial liquid;
10 parts of polypropylene glycol, 10 parts of urea, 8 parts of monoammonium phosphate, 30 parts of traditional Chinese medicine waste residue powder, 20 parts of waste tea residues, 8 parts of fulvic acid concentrated solution, 40 parts of vinasse, 30 parts of leaf mold, 40 parts of sawdust, 20 parts of rice chaff ash, 10 parts of loose phosphorus, 50 parts of coconut chaff and 30 parts of slow release fertilizer;
wherein, the plant straw powder comprises the following components in percentage by weight:
8% of corn stalk powder, 14% of corn cob powder, 25% of potato straw powder, 10% of sea buckthorn straw powder, 10% of alhagi sparsifolia straw powder, 10% of rice straw powder, 8% of flax straw powder, 5% of wheat straw powder, 5% of sorghum straw powder and 5% of pea straw powder;
the microbial liquid is a liquid obtained by culturing and fermenting actinomycetes, azotobacter, bacillus subtilis, photosynthetic bacteria, saccharomycetes, bacillus megaterium and sulfate reducing bacteria;
the algae-containing carrier is a high molecular polymer material loaded with Coccomyxa, Pseudocochlioxa, Lepisorus, Pseudocladocera, Sphingomonas, Microcoleus, Anabaena, Coccidioides and Nostoc.
Preparation method
S31, kaolin and fly ash are mixed uniformly, water is added to be stirred into a dough, and the dough is heated for 1.5 hours at the temperature of 32 ℃ to obtain a first mixture;
s32, mixing the diatomite and the turf uniformly, adding the fulvic acid concentrated solution, adding water, and mixing uniformly (to be in a flowable viscous state) continuously to obtain a second mixture;
s33, adding straw decomposition agent into the plant straw powder, decomposing, mixing with organic fertilizer, humus, traditional Chinese medicine waste residue powder and waste tea residue, stacking, fermenting, mixing with distiller' S grains, leaf mold, sawdust, rice chaff ash and loose phosphorus mixed coconut husk, stirring uniformly, and standing at 25 ℃ for 3 days to obtain a third mixture;
s34, mixing the zeolite powder, the glass beads and the silica powder, adding the hydrophobic resin, and uniformly stirring; obtaining a fourth mixture;
s35, uniformly mixing the second mixture and the third mixture, adding urea, monoammonium phosphate and slow release fertilizer, dispersing and stirring, adding the first mixture and the fourth mixture, uniformly stirring, adding an algae-containing carrier and a microbial liquid, continuously uniformly mixing, paving for 4 hours at 28 ℃ to obtain a pre-used powder material, namely a soil conditioner;
the data of the salt content of the substrate and the data of the germination capacity are obtained in other steps, and a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content of the substrate is constructed, which is the same as the embodiment.
Finally, 17 populus diversifolia seeds in 20 populus diversifolia seeds normally germinate, and a relation curve between the growth rate of the populus diversifolia seeds in a germination period and the salt content of the matrix is established after abnormal seed germination data are removed.
Example 2
Soaking populus diversifolia seeds in a plant nutrient solution for 90min, wherein the plant nutrient solution comprises a populus diversifolia seedling extracting solution; ultrasonic treatment is carried out simultaneously in the soaking process, the power of ultrasonic is 200W, each time of ultrasonic treatment is 8s, and the interval between two adjacent ultrasonic treatments is 30 s;
the plant nutrient solution is conventional commercially available nutrient solution
Constructing a populus diversifolia seed cultivation model: arranging 40 populus diversifolia seeds in a culture medium according to a circle, adding a soil conditioner into the culture medium, wherein the soil conditioner comprises the following raw materials in parts by weight:
the soil conditioner is prepared from the following raw materials: 120 parts of diatomite, 100 parts of fly ash, 80 parts of bentonite, 60 parts of kaolin, 100 parts of turf, 140 parts of plant straw powder, 16 parts of an algae-containing carrier, 50 parts of an organic fertilizer, 13 parts of coated grass seeds, 55 parts of humus, 30 parts of zeolite powder, 25 parts of glass microspheres, 20 parts of silica powder, 4 parts of hydrophobic resin and 25 parts of microbial liquid;
15 parts of polypropylene glycol, 12 parts of urea, 12 parts of monoammonium phosphate, 35 parts of traditional Chinese medicine waste residue powder, 30 parts of waste tea residues, 16 parts of fulvic acid concentrated solution, 50 parts of vinasse, 45 parts of leaf mold, 50 parts of sawdust, 250 parts of rice chaff ash, 20 parts of loose phosphorus, 60 parts of coconut husk and 35 parts of slow release fertilizer.
Wherein, the plant straw powder comprises the following components in percentage by weight:
8% of corn stalk powder, 14% of corn cob powder, 25% of potato straw powder, 10% of sea buckthorn straw powder, 10% of alhagi sparsifolia straw powder, 10% of rice straw powder, 8% of flax straw powder, 5% of wheat straw powder, 5% of sorghum straw powder and 5% of pea straw powder;
the microbial liquid is a liquid obtained by culturing and fermenting actinomycetes, azotobacter, bacillus subtilis, photosynthetic bacteria, saccharomycetes, bacillus megaterium and sulfate reducing bacteria;
the algae-containing carrier is a high molecular polymer material loaded with Coccomyxa, Pseudocochlioxa, Lepisorus, Pseudocladocera, Sphingomonas, Microcoleus, Anabaena, Coccidioides and Nostoc.
The data of the salt content of the substrate and the data of the germination capacity are obtained in other steps, and a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content of the substrate is constructed, which is the same as the embodiment.
Finally, 35 populus diversifolia seeds in 40 populus diversifolia seeds normally germinate, and a relation curve between the growth rate of the populus diversifolia seeds in a germination period and the salt content of the matrix is established after abnormal seed germination data are removed.
Comparative example 1
The poplar seedling extract added in this comparative example was prepared without fermentation, and the other procedures were the same as in example 2.
Finally, 20 populus diversifolia seeds in 40 populus diversifolia seeds normally germinate, and a relation curve between the growth rate of the populus diversifolia seeds in a germination period and the salt content of the matrix is established after abnormal seed germination data are removed.
Comparative example 2
In this comparative example, populus was not soaked in the plant nutrient solution and was not subjected to ultrasonic treatment, and the other procedures were the same as in example 1.
Finally, 7 populus diversifolia seeds in 20 populus diversifolia seeds normally germinate, and a relation curve between the growth rate of the populus diversifolia seeds in a germination period and the salt content of the matrix is established after abnormal seed germination data are removed.
Comparative example 3
In this comparative example, no soil conditioner was added to the medium, and the procedure was otherwise the same as in example 2.
Finally, 23 populus diversifolia seeds in 40 populus diversifolia seeds normally germinate, and a relation curve between the growth rate of the populus diversifolia seeds in a germination period and the salt content of the matrix is constructed after abnormal seed germination data are removed.
While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.
Claims (10)
1. A method for testing the relation between the germination capacity of populus diversifolia seeds and the salt content of a matrix is characterized by comprising the following steps:
populus diversifolia seed treatment: soaking populus diversifolia seeds in a plant nutrient solution for 10min-120min, wherein the plant nutrient solution comprises a populus diversifolia seedling extracting solution; ultrasonic treatment is carried out simultaneously in the soaking process, the power of the ultrasonic is 100-200W, each time of ultrasonic treatment is 5-8s, and the interval between two adjacent ultrasonic treatments is 20-30 s;
constructing a populus diversifolia seed cultivation model: the method comprises the following steps of arranging populus diversifolia seeds in a circular shape in a culture medium, and adding a soil conditioner into the culture medium, wherein the soil conditioner comprises the following raw materials in parts by weight:
100-150 parts of diatomite, 80-120 parts of fly ash, 70-90 parts of bentonite, 50-70 parts of kaolin, 80-120 parts of turf, 120-160 parts of plant straw powder, 12-20 parts of algae-containing carriers, 40-60 parts of organic fertilizer, 40-70 parts of humus, 20-35 parts of zeolite powder, 20-30 parts of glass beads, 18-25 parts of silica powder, 3-5 parts of hydrophobic resin and 20-30 parts of microbial liquid;
10-20 parts of polypropylene glycol, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of traditional Chinese medicine waste residue powder, 20-40 parts of waste tea residues, 8-24 parts of fulvic acid concentrated solution, 40-60 parts of vinasse, 30-60 parts of leaf mold, 40-60 parts of sawdust, 20-30 parts of rice chaff ash, 10-30 parts of phosphorus, 50-70 parts of coconut husk and 30-40 parts of slow release fertilizer;
acquiring germination capacity data: in a populus diversifolia seed germination period, acquiring a time three-dimensional model diagram of a populus diversifolia seed cultivation model to be detected through a three-dimensional scanner; the time three-dimensional model map comprises three-dimensional model maps corresponding to a plurality of populus diversifolia seed cultivation models to be detected at different moments; analyzing and processing through a time three-dimensional model diagram, and further calculating to obtain the growth rate of the populus euphratica seeds in the populus euphratica seed cultivation model in a germination period;
obtaining the salt content data of the matrix: collecting a culture medium sample around the populus euphratica seeds in the populus euphratica seed cultivation model to be detected in a populus euphratica seed germination period; measuring the content data of the salt in the culture matrix sample around the populus diversifolia seeds by using a salt tester, and calculating the content data of the salt in the culture matrix sample around the populus diversifolia seeds to obtain the content data of the salt in the matrix;
and constructing a relation curve between the growth rate of populus euphratica seeds in a germination period and the salt content of the substrate.
2. The method for testing the relationship between the germination capacity and the salt content of the medium of populus diversifolia seeds as claimed in claim 1, wherein in the populus diversifolia seed cultivation model, the distance between adjacent populus diversifolia seeds is not more than 15cm, and the number of populus diversifolia seeds in the culture medium is not more than 40;
and in the process of acquiring the germination capacity data, rejecting abnormal germination capacity data of populus diversifolia seeds.
3. The method for testing the relationship between the germination capacity and the salt content of the medium of populus diversifolia seeds as claimed in claim 2, wherein the analysis and the processing are performed through a time three-dimensional model map, and the growth rate of the populus diversifolia seeds in the populus diversifolia seed cultivation model in the germination period is further calculated and obtained, and the method comprises the following steps:
acquiring a three-dimensional coordinate of a time three-dimensional model diagram to obtain a vertex coordinate in an XYZ three-axis direction;
and (3) calculating the growth rate of the populus diversifolia seeds in the germination period according to the vertex coordinates:
in the formula, VtIs the growth rate of populus diversifolia seeds in a germination period;
λ1、λ2、λ3、λ4、λ5、λ6are all distance difference square coefficients, where1、λ2、λ4、λ5The value range of (A) is between 0.95 and 1.05, lambda3The value range of (A) is between 0.27 and 0.67, lambda6The value range of (1) is 1.84-3.89;
Xt maxthe X coordinate value of the maximum value point in the X axis direction at the time t on the time three-dimensional model graph is obtained;
Xt+1 maxthe X coordinate value of the maximum value point in the X axis direction at the time t + △ t on the time three-dimensional model graph;
Yt maxthe Y coordinate value of the maximum value point in the Y axis direction at the time t on the time three-dimensional model graph is obtained;
Yt+1 maxthe Y coordinate value of the maximum value point in the Y-axis direction at the time t + △ t on the time three-dimensional model graph;
Zt maxthe Z coordinate numerical value of the maximum value point in the Z axis direction at the time t on the time three-dimensional model graph is obtained;
Zt+1 maxthe Z coordinate value of the maximum value point in the Z axis direction at the time t + △ t on the time three-dimensional model diagram;
Xt minthe X coordinate value of the minimum value point in the X axis direction at the time t on the time three-dimensional model graph is obtained;
Xt+1 minthe X coordinate value of the minimum value point in the X axis direction at the time t + △ t on the time three-dimensional model graph;
Yt minthe Y coordinate value of the minimum value point in the Y axis direction at the time t on the time three-dimensional model graph is obtained;
Yt+1 minthe Y coordinate value of the minimum value point in the Y-axis direction at the time t + △ t on the time three-dimensional model graph;
Zt minthe Z coordinate value of the minimum value point in the Z axis direction at the time t on the time three-dimensional model graph is obtained;
Zt+1 minis the Z coordinate value of the minimum value point in the Z axis direction at the time t + △ t on the time three-dimensional model diagram.
4. The method for testing the relationship between the germination capacity and the salt content of the medium of populus diversifolia seeds as claimed in claim 2, wherein the analysis and the processing are performed through a time three-dimensional model map, and the growth rate of the populus diversifolia seeds in the populus diversifolia seed cultivation model in the germination period is further calculated and obtained, and the method comprises the following steps:
acquiring a three-dimensional coordinate of a time three-dimensional model diagram, and further acquiring a projection area of the three-dimensional model diagram in the direction of XYZ three axes;
calculating the growth rate of the populus diversifolia seeds in the germination period according to the projection area in the direction of three XYZ axes:
in the formula, VtIs the growth rate of populus diversifolia seeds in a germination period; mu.s1、μ2、μ3Are all projection area difference square coefficients, where1、μ2The value range of (1) is between 0.72 and 0.86 mu3The value range of (A) is between 0.94 and 1.17;
St Xthe projection area of the time three-dimensional model diagram t moment in the X-axis direction is shown;
St Ythe projection area of the time three-dimensional model diagram t moment in the Y-axis direction is shown;
St Zthe projection area of the time three-dimensional model diagram t moment in the Z-axis direction is shown;
St+1 Xthe projection area of the time three-dimensional model diagram t + △ t in the X-axis direction is shown;
St+1 Ythe projection area of the time three-dimensional model diagram t + △ t in the Y-axis direction is shown;
St+1 Zis the projected area in the Z-axis direction at the time of the time three-dimensional model map t + △ t.
5. The method for testing the relationship between the germination capacity of populus diversifolia seeds and the salt content of the medium according to any one of claims 3 to 4, wherein the collection positions of the culture medium samples are the upper end, the lower end, the left end, the right end, the front end and the rear end of the central coordinates of the populus diversifolia seeds.
6. The method for testing the relationship between the germination capacity of populus diversifolia seeds and the content of the matrix salt in the populus diversifolia seeds according to claim 5, wherein the data of the content of the matrix salt is calculated by the following formula:
Ct＝Kon the upper part*Wt On the upper part+KLower part*Wt Lower part+KLeft side of*Wt Left side of+KRight side*Wt Right side+KFront side*Wt Front side+KRear end*Wt Rear end
In the formula, CtThe salt content of the matrix; kOn the upper part、KLower part、KLeft side of、KRight side、KFront side、KRear endAll are salt content coefficients; wherein, KLeft side of、KRight side、KFront side、KRear endThe value range of (A) is between 0.87 and 1.12; kOn the upper partThe value range of (A) is between 0.24 and 0.32; kLower partThe value range of (A) is between 1.37 and 1.87;
Wt on the upper partFor the matrix sample M at time tOn the upper partMedium salinity content data;
Wt lower partFor the matrix sample M at time tLower partMedium salinity content data;
Wt left side ofFor the matrix sample M at time tLeft side ofMedium salinity content data;
Wt right sideFor the matrix sample M at time tRight sideMedium salinity content data;
Wt front sideFor the matrix sample M at time tFront sideMedium salinity content data;
Wt rear endFor the matrix sample M at time tRear endMedium salinity content data;
wherein M isOn the upper partA substrate sample representing the upper end of the central coordinates of populus euphratica seeds;
Mlower partA substrate sample representing the lower end of the central coordinates of the populus euphratica seeds;
Mleft side ofA substrate sample representing the left end of the central coordinates of populus euphratica seeds;
Mright sideA substrate sample representing the right end of the central coordinates of the populus euphratica seeds;
Mfront sideA substrate sample representing the front end of the central coordinates of the populus euphratica seeds;
Mrear endRepresenting Populus euphratica seedsMatrix samples behind the center coordinates.
7. The method for testing the germination capacity of populus diversifolia seeds as a function of the salt content of the medium of claim 6, wherein after constructing a salt calibration model based on the growth rate of populus diversifolia seeds during the germination cycle and the salt content of the medium of the populus diversifolia seeds, the salt calibration model is constructed:
collecting culture medium samples of the upper end, the lower end, the left end, the right end, the front end and the rear end of central coordinates of adjacent seeds of the populus diversifolia seeds in a germination period of the populus diversifolia seeds, measuring salt content data in the culture medium samples of the adjacent seeds by a salt tester, and then calculating to obtain a salt correction coefficient;
and correcting a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content of the matrix according to the salt correction coefficient.
8. The method for testing the relationship between the germination capacity of populus diversifolia seeds and the salt content of the matrix according to claim 7, wherein the salt content data of the matrix is calculated by the following formula:
in the formula, KOn the upper part、KLower partIs the salt content coefficient; kOn the upper partThe value range of (A) is between 0.24 and 0.32; kLower partThe value range of (A) is between 1.37 and 1.87;
Hon the upper partFor culturing the substrate sample MOn the upper partCollecting the vertical distance from the position to the surface of the substrate;
Hlower partFor culturing the substrate sample MLower partThe perpendicular distance of the collection location from the substrate surface.
9. The method for testing the relationship between the germination capacity and the content of matrix salt of populus diversifolia seeds as claimed in claim 8, wherein the measurement of the germination capacity data is performed on a model established in 3-matic forward design software.
10. The method for testing the relationship between the germination capacity and the matrix salt content of populus diversifolia seeds as claimed in claim 9, wherein the temperature correction model and the moisture correction model are constructed after the relationship curve is constructed according to the growth rate and the matrix salt content data of the populus diversifolia seeds in the germination period, and the temperature correction coefficient and the moisture correction coefficient are obtained by the temperature correction model and the moisture correction model;
and correcting a relation curve between the growth rate of the populus diversifolia seeds in the germination period and the salt content of the matrix according to the temperature correction coefficient and the moisture correction coefficient.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901171A (en) * | 2014-04-11 | 2014-07-02 | 河南农业大学 | Method for evaluating flooding tolerance of maize variety |
| KR20150000537A (en) * | 2013-06-24 | 2015-01-05 | 대한민국(농촌진흥청장) | A method for enhancing salt stress resistance of plant using sound wave |
| CN104221678B (en) * | 2014-09-12 | 2016-04-13 | 谢振西 | A kind of method improving Hua Shallot seed seedling-raising survival rate |
| CN106472142A (en) * | 2016-10-18 | 2017-03-08 | 湖南农业大学 | A kind of resistance to screening technique for flooding flax kind |
| CN106495949A (en) * | 2016-10-26 | 2017-03-15 | 广西鑫雅皇庭园林工程有限责任公司 | Soil desertification biological modification agent and preparation method thereof, using method |
| CN106810326A (en) * | 2017-03-30 | 2017-06-09 | 山东农业大学 | A kind of method for improving clover salt resistance ability |
| CN106950262A (en) * | 2017-04-24 | 2017-07-14 | 江苏大学 | A kind of method for predicting crop salt stress level |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110162116A1 (en) * | 2008-06-16 | 2011-06-30 | Holman Edwin Henricus Antonius | Method For Growing Plants |
-
2017
- 2017-08-24 CN CN201710734329.4A patent/CN109425700B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150000537A (en) * | 2013-06-24 | 2015-01-05 | 대한민국(농촌진흥청장) | A method for enhancing salt stress resistance of plant using sound wave |
| CN103901171A (en) * | 2014-04-11 | 2014-07-02 | 河南农业大学 | Method for evaluating flooding tolerance of maize variety |
| CN104221678B (en) * | 2014-09-12 | 2016-04-13 | 谢振西 | A kind of method improving Hua Shallot seed seedling-raising survival rate |
| CN106472142A (en) * | 2016-10-18 | 2017-03-08 | 湖南农业大学 | A kind of resistance to screening technique for flooding flax kind |
| CN106495949A (en) * | 2016-10-26 | 2017-03-15 | 广西鑫雅皇庭园林工程有限责任公司 | Soil desertification biological modification agent and preparation method thereof, using method |
| CN106810326A (en) * | 2017-03-30 | 2017-06-09 | 山东农业大学 | A kind of method for improving clover salt resistance ability |
| CN106950262A (en) * | 2017-04-24 | 2017-07-14 | 江苏大学 | A kind of method for predicting crop salt stress level |
Non-Patent Citations (3)
| Title |
|---|
| 植物营养液浸种与喷施对水稻产量效应的初步研究;徐宗俦等;《种子》;19981231(第3期);第54页右栏3.1 * |
| 沙枣和柠条种子萌发期耐盐性研究;刘宝玉等;《植物研究》;20071130;第27卷(第6期);第722页右栏1.2处理方法，1.3指标测定，第724页右栏图4和图5 * |
| 种子活力保持与提高技术研究进展;崔婷等;《作物研究》;20141231;第28卷(第4期);第438页左栏2.3.3生物方法 * |
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