CN106810326A - A kind of method for improving clover salt resistance ability - Google Patents

A kind of method for improving clover salt resistance ability Download PDF

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Publication number
CN106810326A
CN106810326A CN201710200692.8A CN201710200692A CN106810326A CN 106810326 A CN106810326 A CN 106810326A CN 201710200692 A CN201710200692 A CN 201710200692A CN 106810326 A CN106810326 A CN 106810326A
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salt
mus
clover
soil
resistance ability
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Inventor
娄燕宏
晁赢
诸葛玉平
宋付朋
王会
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Shandong Agricultural University
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Shandong Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to salt-soda soil evaluation and exploration technology field, there is provided a kind of method of raising clover salt resistance ability, comprise the following steps that:1. alfalfa cultivation:A, seed selection;B, soil treatment;C, sowing;2. cultivation management:A, root apply alkali solution of beet, and glycine betaine is made into the concentration of 5mmol/L, are applied according to every mu of amount of 100kg in seedling stage;B, liquid manure are planned strategies for, key developmental stages fertilising, irrigation.The present invention is by selecting salt-enduring cultivars, soil is deep ploughed, the various control salt of stalk deep placement also field, balance fertilizing etc., many behaves of agronomic measures of suppression salt are laid equal stress on, glycine betaine technology is applied with reference to root, the salt resistance ability of clover is effectively improved, it is easy to operate, instant effect, strong operability, it is with low cost, salt-soda soil clover volume increase can be effectively facilitated.

Description

A kind of method for improving clover salt resistance ability
Technical field
The present invention relates to salt-soda soil evaluation and exploration technology field, there is provided a kind of method of raising clover salt resistance ability.
Background technology
The soil salinization is the main adverse circumstance of limitation crop production in global range, the whole world about 9.5 hundred million hm2Soil By saliferous threat, the 5% of world arable land is accounted for, especially with increasingly sharpening for global warming trend, Mid-low latitude The soil salinization problem in area becomes clear day by day.Salt-soda soil as the important reserve land resources for being available for and developing, to it Develop of far-reaching significance.Saline-alkaline Soils of Yellow River Delta, its production efficiency is low, and land quality is low, a large amount of soil caused by uncultivated The ground wasting of resources, causes overall agricultural productivity wretched insufficiency, serious restriction our province agricultural sustainable development.
Clover (Medicago sativa L.) is used as a kind of perennial high-quality legume forage, its strong adaptability, nutriture value Value is high, is described as " King of Pasture ", it has also become the chief crop kind of the structural readjustment of rural industry.Meanwhile, clover can also change Kind soil physics structure and fertility, increase ground mulching degree, with good ecological benefits and economic benefit.In recent years, lucerne Status of the Mu in grassland industry development is increasingly taken seriously, and especially needs to pay attention to grain in salt-soda soil develops through raising system Raise, under the guilding principle that army provisions are taken into account, the alfalfa cultivation in salt-soda soil is more particularly important.But, due to being coerced by salinity Compel, the growth of clover is suppressed, and yield is remarkably decreased, seriously constrain the alfalfa industry development in salt-soda soil, and then for poultry The stable development of animal husbandry produces ineffective effect, therefore, needing research and development badly can improve the technological means and method of clover salt resistance ability.
The content of the invention
For many weak points that prior art is present, the invention provides a kind of side for improving clover salt resistance ability Method, comprises the following steps that:1. alfalfa cultivation:A, seed selection;B, soil treatment;C, sowing;2. cultivation management:A, root apply beet alkali soluble Liquid, glycine betaine is made into the concentration of 5mmol/L, is applied according to every mu of amount of 100kg in seedling stage;B, liquid manure are planned strategies for, key fertility Phase fertilising, irrigation.The present invention is deep ploughed by selecting salt-enduring cultivars, soil, the various control salt of stalk deep placement also field, balance fertilizing etc., The many behaves of agronomic measures for pressing down salt are laid equal stress on, and glycine betaine technology is applied with reference to root, effectively improve the salt resistance ability of clover, easy to operate, Instant effect, strong operability is with low cost, can effectively facilitate salt-soda soil clover volume increase.
Concrete technical scheme of the invention is:
A kind of method for improving clover salt resistance ability, comprises the following steps:
1. alfalfa cultivation
A, seed selection
B, soil treatment
C, sowing
2. cultivation management
A, root apply alkali solution of beet
B, liquid manure are planned strategies for.
Specifically the step of, is as follows:
1. alfalfa cultivation
A, seed selection
From the stronger alfalfa variety of salt tolerance, such as middle lucerne 1, the star of salt tolerant, Ao Han, imperial crown, middle lucerne 3.
B, soil treatment
35~40cm of soil deep ploughing before plantation, loose table soil, rakes in time;Dig trunk canal and branch canal, it is ensured that draining is unimpeded; Deep layer applies crop material, 25~30cm of depth, and consumption is 2.5~3.0kg/m2
Using base manure:Organic fertilizer and inorganic fertilizer compounding application, Rate of Organic Fertilizer are 1000~2000kg/ mus;
Inorganic fertilizer:5~15kg/ of urea mus, 50~100kg/ of calcium superphosphate mus of 18wt%, the potassium sulfate 20 of 51wt%~ 40kg/ mus, 3~5kg/ of magnesium sulfate mus, 0.5~1.5kg/ of Zinc sulfate monohydrate mus, 0.5~1.0kg/ of borax mus, ammonium molybdate 5~ 10g/ mus;
C, sowing
Selection carries out autumn sowing to mid-September by the end of August, and seed first carries out selected impurity elimination, and every kilogram of seed nitragin 3~ Sowed immediately after 7g Rhizobium Inoculations, drilling, 1.0~1.5kg of every mu of application rate, 30~35cm of line-spacing, depth of planting 1.5~ 2.5cm;
2. cultivation management:
A, root apply alkali solution of beet
Glycine betaine is made into the aqueous solution of 5mmol/L, is applied according to every mu of amount of 100kg in seedling stage;
B, liquid manure are planned strategies for
If after planting more than three days need to irrigate in time without rainfall, irrigation is advisable with drip irrigation or micro- spray, need to moisten 0~5cm tables Layer soil;Topdressed with reference to irrigation in time after cradling every time, consumption is respectively 2~4kg/ of urea mus, the peroxophosphoric acid of 18wt% 40~50kg/ of calcium mus, 8~10kg/ of potassium sulfate mus of 51wt%.
In addition, other planting measures use routine techniques.
In the present invention, inventor applies crop material in soil treatment in deep layer, thus can be by soil capillary The progressive failure of effect, salinity runs up to earth's surface with capillary rising in preventing subsoil or underground water;
Selected crop material is mainly selected from the stalk or its mixture of paddy rice or wheat or corn;Organic fertilizer is selected from pig Excrement or cow dung or its mixture.
Compared with prior art, the present invention has following advantages and good effect:
1. imposed on clover using glycine betaine root, improve clover overground part relative growth rate, underground part relative growth Speed and superoxide dismutase activity, reduce MDA and soluble protein content, significantly improve the salt tolerant energy of clover Power, easy to operate, instant effect, strong operability is with low cost;And present invention determine that optimal use concentration is the water of 5mmol/L Solution, excessive concentration will not only improve salt tolerance, can also suppress alfalfa growing;Concentration is too low, does not have and improves salt tolerance Ability;
2. Mixed application of organic and inorganic fertilizers effectively improves soil texture as base manure, reduces topsoil soils salinity; Balance fertilizing, based on phosphorus potash fertilizer, is aided with a small amount of nitrogenous fertilizer, and compounding application trace-element fertilizer meets alfalfa growing nutrient demand While, improve resistance;
3. soil deep ploughing, effectively cut-out subsoil capillary, suppress the topsoil accumulation of salt in the surface soil;Stalk deep placement also field, also may be used By the progressive failure of soil capillarity, salinity runs up to earth's surface with capillary rising in preventing subsoil or underground water;Together When be also used as organic fertilizer for alfalfa growing provides nutrient;
4. salt-enduring cultivars are selected, with reference to various control salt, the agronomic measures of suppression salt, fit applications improve the skill of clover salt tolerance Art means, many behaves are laid equal stress on, and effectively improve the salt resistance ability of clover, promote the volume increase of salt-soda soil clover.
Specific embodiment
Embodiment 1
Greenhouse manual control environment's betaine administration improves the salt tolerance of clover, and main step is poly- as follows:
1st, alfalfa cultivation
It is material from alfalfa variety ' star of salt tolerant ', be seeded in the plastic cup equipped with fine sand (upper bore 6.8cm, under Bore 4.8cm, 7.4cm high), husky 1cm is after planting covered, covered with three layers of toilet paper, 1/2Hoagland nutrient solutions are poured, keep wet Profit is placed in human controllable greenhouse and cultivates up to emerging, and temperature is 25 DEG C of daytime, 20 DEG C of evening, light application time 16h, humidity 50%, intensity of illumination is 600 μm of ol/m2·s。
2nd, glycine betaine optimal application concentration determines
Whne clover it is long to 45 days seedling ages when, clover is taken out, clean sand, be transferred in 250mL triangular flasks.In triangular flask 1/2Hoagland nutrient solutions equipped with 1% salinity, and certain density glycine betaine is added respectively, glycine betaine addition concentration sets It is set to:0th, 5,10,20mM, triangular flask is placed in illumination box and is cultivated 6 days, and temperature is 25 DEG C of daytime, 20 DEG C of evening, illumination Time 16h, humidity 50%, intensity of illumination is 300 μm of ol/m2S, determines the growth indexes and activities of antioxidant enzymes of each treatment.
Result of the test:Under salt stress, addition glycine betaine can improve the salt resistance ability of clover, be embodied in and improve ground Top relative growth rate, underground part relative growth rate and superoxide dismutase activity, reduce MDA and solubility Protein content.Wherein, the clover of addition 5mM beet alkali process shows overground part relative growth rate, the underground part phase of maximum To growth rate, biomass, activity of SOD, peroxidase activity, and relative transpiration rate;With minimum MDA and soluble protein content, therefore, addition 5mM glycine betaines have to the salt tolerance of the clover under 1% salt stress compared with Good raising effect.
Embodiment 2
Crop field natural environment betaine administration improves the salt tolerance of clover, and operating procedure is as follows:
From the star of the stronger alfalfa variety-salt tolerant of salt tolerance.Plot surrounding drainage trenching, it is smooth to ensure plot draining It is logical.Deep ploughing more than soil 35cm, deep layer (25cm) applies wheat stalk (with 3.0kg/m2Meter).Every mu is applied 1500kg organic fertilizers (cow dung), urea 10kg, calcium superphosphate 75kg, potassium sulfate 30kg, magnesium sulfate 4kg, Zinc sulfate monohydrate 1.0kg, borax 1.0kg, ammonium molybdate 7.5g, rotary tillage 10cm rake in time;
It is mixed with nitragin (powdery, every kilogram of seed consumption is 5g rhizobium) after August 25 days is by the artificial impurity elimination of seed Seed dressing is closed, the same day is sowed, and drilling, application rate is every mu of 1.2kg, and line-spacing is 30cm, depth of planting 2cm;
Irrigated using micro- spirt row within after planting the 3rd day, moisten topsoil 5cm, irrigated again after three days, until emerging, gone out After seedling 25 days, every mu of root applies the glycine betaine 100kg of 5mmol/L, carries out imposing azophoska, nitrogenous fertilizer with reference to irrigation after next year results From urea, consumption is 6.5kg/ mu, phosphate fertilizer ssp dnab, and consumption is 45kg/ mus, and potash fertilizer selects the potassium sulfate, consumption to be 9kg/ mus.
Meanwhile, it is control with the clover that local Routine Management mode is planted, result of the test finds to improve the growth of clover Speed, its grass yield more conventional management improves 7.3%.
Embodiment 3
Crop field natural environment betaine administration improves the salt tolerance of clover, and operating procedure is as follows:
From the stronger alfalfa variety of salt tolerance-middle lucerne one.Plot surrounding drainage trenching, it is smooth to ensure plot draining It is logical.Deep ploughing more than soil 35cm, deep layer (25cm) applies rice straw (with 3.0kg/m2Meter).Every mu is applied 1500kg organic fertilizers (pig manure), urea 10kg, calcium superphosphate 75kg, potassium sulfate 30kg, magnesium sulfate 4kg, Zinc sulfate monohydrate 1.0kg, borax 1.0kg, ammonium molybdate 7.5g, rotary tillage 10cm rake in time;
It is mixed with nitragin (powdery, every kilogram of seed consumption is 5g rhizobium) after August 25 days is by the artificial impurity elimination of seed Seed dressing is closed, the same day is sowed, and drilling, application rate is every mu of 1.2kg, and line-spacing is 30cm, depth of planting 2cm;
Irrigated using micro- spirt row within after planting the 3rd day, moisten topsoil 5cm, irrigated again after three days, until emerging, gone out After seedling 25 days, every mu of root applies the glycine betaine 100kg of 5mmol/L, carries out imposing azophoska, nitrogenous fertilizer with reference to irrigation after next year results From urea, consumption is 6.5kg/ mu, phosphate fertilizer ssp dnab, and consumption is 45kg/ mus, and potash fertilizer selects the potassium sulfate, consumption to be 9kg/ mus.
Meanwhile, it is control with the clover that local Routine Management mode is planted, result of the test shows that the salt tolerance of clover is notable Improve, growth rate substantially accelerates, the more conventional management of its grass yield improves 6.90%.

Claims (3)

1. it is a kind of improve clover salt resistance ability method, it is characterised in that:Comprise the following steps:
1. alfalfa cultivation
A, seed selection
From the stronger alfalfa variety of salt tolerance;
B, soil treatment
35~40cm of soil deep ploughing before plantation, loose table soil, rakes in time;Dig trunk canal and branch canal, it is ensured that draining is unimpeded;Deep layer Crop material, 25~30cm of depth are applied, consumption is 2.5~3.0kg/m2
Using base manure:Organic fertilizer and inorganic fertilizer compounding application, Rate of Organic Fertilizer are 1000~2000kg/ mus;
Inorganic fertilizer:5~15kg/ of urea mus, 50~100kg/ of calcium superphosphate mus of 18wt%, the potassium sulfate 20 of 51wt%~ 40kg/ mus, 3~5kg/ of magnesium sulfate mus, 0.5~1.5kg/ of Zinc sulfate monohydrate mus, 0.5~1.0kg/ of borax mus, ammonium molybdate 5~ 10g/ mus;
C, sowing
Selection carries out autumn sowing to mid-September by the end of August, and seed first carries out selected impurity elimination, and every kilogram of 3~7g of seed nitragin connects Sowed immediately after planting rhizobium, drilling, 1.0~1.5kg of every mu of application rate, 30~35cm of line-spacing, 1.5~2.5cm of depth of planting;
2. cultivation management:
A, root apply alkali solution of beet
Glycine betaine is made into the aqueous solution of 5mmol/L, is applied according to every mu of amount of 100kg in seedling stage;
B, liquid manure are planned strategies for
If after planting more than three days need to irrigate in time without rainfall, irrigation is advisable with drip irrigation or micro- spray, need to moisten 0~5cm topsoils Earth;Topdressed with reference to irrigation in time after cradling every time, consumption is respectively 2~4kg/ of urea mus, the calcium superphosphate 40 of 18wt% ~50kg/ mus, 8~10kg/ of potassium sulfate mus of 51wt%.
2. it is according to claim 1 improve clover salt resistance ability method, it is characterised in that:The stronger lucerne of the salt tolerance The star or Ao Han or imperial crown or middle lucerne 3 of lucerne 1 or salt tolerant in the trade name of Mu kind.
3. it is according to claim 1 improve clover salt resistance ability method, it is characterised in that:The work selected during soil treatment Thing stalk is selected from the stalk or its mixture of paddy rice or wheat or corn;Organic fertilizer is selected from pig manure or cow dung.
CN201710200692.8A 2017-03-30 2017-03-30 A kind of method for improving clover salt resistance ability Pending CN106810326A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108243859A (en) * 2017-12-30 2018-07-06 广东红树林生态科技有限公司 A kind of method of quickly breeding salt tolerant autumn eggplant
CN108521925A (en) * 2018-05-08 2018-09-14 东北农业大学 A method of administering saline-alkali grassland using yellow storage stalk
CN109169063A (en) * 2018-09-17 2019-01-11 山东农业大学 A kind of synthetic method for planting improving salt-soda soil millet salt tolerance
CN109425701A (en) * 2017-08-24 2019-03-05 中国科学院寒区旱区环境与工程研究所 A method of test diversiform-leaved poplar seed sprouts ability and water supply in media relation with contents
CN109425700A (en) * 2017-08-24 2019-03-05 中国科学院寒区旱区环境与工程研究所 A method of test diversiform-leaved poplar seed sprouts ability and matrix salt content relationship
CN109729956A (en) * 2018-12-26 2019-05-10 中国科学院植物研究所 A kind of salt resistance matrix and its application for cultivating clover

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1275051A (en) * 1997-07-03 2000-11-29 魁北克大学蒙特利尔分校 Method for improving the freezing tolerance of plant
CN105123231A (en) * 2015-09-08 2015-12-09 定西华冠农牧业开发有限公司 Micro-irrigation planting method for alfalfa

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1275051A (en) * 1997-07-03 2000-11-29 魁北克大学蒙特利尔分校 Method for improving the freezing tolerance of plant
CN105123231A (en) * 2015-09-08 2015-12-09 定西华冠农牧业开发有限公司 Micro-irrigation planting method for alfalfa

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109425701A (en) * 2017-08-24 2019-03-05 中国科学院寒区旱区环境与工程研究所 A method of test diversiform-leaved poplar seed sprouts ability and water supply in media relation with contents
CN109425700A (en) * 2017-08-24 2019-03-05 中国科学院寒区旱区环境与工程研究所 A method of test diversiform-leaved poplar seed sprouts ability and matrix salt content relationship
CN109425700B (en) * 2017-08-24 2020-08-14 中国科学院西北生态环境资源研究院 Method for testing relation between populus diversifolia seed germination capacity and medium salt content
CN109425701B (en) * 2017-08-24 2020-08-21 中国科学院西北生态环境资源研究院 Method for testing relation between populus diversifolia seed germination capacity and substrate moisture content
CN108243859A (en) * 2017-12-30 2018-07-06 广东红树林生态科技有限公司 A kind of method of quickly breeding salt tolerant autumn eggplant
CN108243859B (en) * 2017-12-30 2020-10-13 广东红树林生态科技有限公司 Method for rapidly cultivating salt-tolerant Kandelia candel
CN108521925A (en) * 2018-05-08 2018-09-14 东北农业大学 A method of administering saline-alkali grassland using yellow storage stalk
CN109169063A (en) * 2018-09-17 2019-01-11 山东农业大学 A kind of synthetic method for planting improving salt-soda soil millet salt tolerance
CN109169063B (en) * 2018-09-17 2021-01-15 山东农业大学 Comprehensive cultivation method for improving salt tolerance of millet in saline-alkali soil
CN109729956A (en) * 2018-12-26 2019-05-10 中国科学院植物研究所 A kind of salt resistance matrix and its application for cultivating clover

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Application publication date: 20170609