KR20150000537A - A method for enhancing salt stress resistance of plant using sound wave - Google Patents

Abstract

The present invention relates to a method for promoting plant resistance to salt stress wherein the plant is processed by sound waves. Under the condition of salt stress, the plants processed with sound waves smoothly absorb water and nutrients and has less damage to a photosynthetic apparatus, and seeds processed with sound waves have a high germination rate. Therefore, the method of the present invention can be used for manufacturing plants and seeds with resistance to salt stress.

Description

[0001] The present invention relates to a method for enhancing salt stress resistance of a plant using a sound wave,

The present invention relates to a method for treating a plant with a specific range of sound waves to have tolerance to salt stress.

Agriculture has unique risks and instabilities that are not found in other industries. Agriculture is not only uncertain because of the large loss of catastrophes as well as large fluctuations in agricultural prices and the relatively low price of agricultural produce compared to the purchase price of production materials. Agriculture is a natural hazard, and it is more vulnerable to damage than any other industry by a pair of floods, frosts, and hail. This is because agricultural production fields and crops are constrained because they are closely connected to land that can not be moved, and because the object of production is an animal or a plant, it is dominated by natural conditions according to the constraints of life phenomena.

The salinization of crops occurs when crops are cultivated in salt and sodium soils. It is caused by the inflow of seawater, the concentration of salts by the evaporation of soil water, the irrigation of high salinity irrigation water, It is also affected by the effect of sea water droplets due to storm or wave. In Korea, the three sides are surrounded by the sea, and most of them are composed of coasts, and there are many salt sea areas. The salinity was increased by the intrusion of salt water and the evaporation of soil moisture. As a result, the soil was damaged due to the increase of salt concentration. Some areas were used as a salt field and much of it has been turned into a reclaimed land since the 1960s. Many of them have been matured to increase the yield of rice. However, in the case of a new rice cultivation project which has been completed in recent years, there are many cases where the rice is damaged.

It is known that plants have a variety of mechanisms that allow them to recognize when they are exposed to these external environments and to resist the adverse environmental conditions through the transmission of these signals into cells. Research and technological developments have been attempted to artificially stimulate these signal transduction mechanisms to induce self defense mechanisms or regulation of growth responses of plants. As a part of research on plant reaction mechanism by artificial external signal processing, some researches on the response to the plant response to mechanical stimuli have been reported. In case of 50 Hz sound wave treatment, the length of hypocotyl length in cucumber, There was a report that growth was promoted. Domestic cucumbers have also been reported to have a strong effect on the growth of pests when treated with sonic waves. The mechanism by which plants sense and respond to external signals will be through a very complicated process, and the variation in the amount of gene expression will ultimately result in changes in physiological and metabolism of plants. The results of this study are rarely reported. The touch (TCH) gene has been reported as a gene expressed in response to mechanical stimuli such as touch or vibration in Arabidopsis, but the precise mechanism for this is still being studied. There is no report on the results of studies that have been resistant to salt stress, which is one of environmental stress, through specific sound wave processing, which is an external signal.

Accordingly, the inventors of the present invention have completed the present invention by confirming that the plant is treated with a sound wave of a specific range to increase resistance to salt stress and promote seed germination.

It is an object of the present invention to provide a method for enhancing salt stress resistance of plants using sound waves.

In order to achieve the above object, the present invention provides a method for enhancing the salt stress resistance of plants using sound waves.

The use of the method of enhancing the salt stress resistance of a plant using the sound wave of the present invention has a good growth condition under salt stress conditions and promotes germination of the seeds. Therefore, in order to improve the resistance to poor environmental stress, This is a breakthrough technology that can be realized without complex processes such as conversion body building methods.

Fig. 1 is a photograph showing the growth state of the rice seedlings treated with sonication under the salt stress condition; Fig.
0 mM NaCl: salt-stressed control;
No sound: unprocessed rice seedlings; And
Sound: sonicated rice seedlings.
FIG. 2 is a graph showing the roots weight (in freshness) of the sonicated rice seedlings under salt stress conditions.
Fig. 3 is a photograph showing the root lengths of the sonicated rice seedlings under salt stress conditions.
FIG. 4 is a graph showing root lengths of rice seedlings treated with sonication under salt stress conditions.
FIG. 5 is a graph showing moisture content of roots in a salt stress condition of rice seedlings treated with sonication.
FIG. 6 is a graph showing the relative moisture content of the leaves under the salt stress conditions of sonicated rice seedlings.
FIG. 7 is a graph showing the maximum chlorophyll fluorescence (Fv / Fm) of the leaves under the salt stress condition of sonicated rice seedlings.

Hereinafter, the present invention will be described in detail.

A sound wave is a phenomenon in which a compressed portion having a relatively high air density formed by the vibration of an object and a lean portion having a low air density are propagated to the outside. Depending on the frequency, the sound waves are classified into audible waves having a frequency of about 20 to 20,000 Hz, infrasonic waves having a frequency of 20 Hz or less, and ultrasonic waves having a frequency of 20,000 Hz or more. In general, music is composed of compound sound waves. In the present invention, sound waves of a specific range other than a complex sound wave are used. Specific sound processing is performed by using Adoba Audition 3.0 software (Adobe System Company) A specific sound wave was applied to the plant through the mounted speaker.

(Fv / Fm) used in the present invention means the maximum quantum yield (Fv / Fm) for the photochemical reaction when the plant leaves maximize photosynthesis. These values are called maximum or potential quantum yields in photochemical reactions and are sometimes referred to as photochemical efficiencies. Fv / Fm is also an indicator widely used in stress physiology because the value also has a meaning indicating the degree of stress.

The present invention provides a method for enhancing the salt stress resistance of a plant using a sound wave including treating a plant with a sound wave of 500 Hz to 1000 Hz.

It is preferable that the sound wave process is performed at a sound level of 50 to 100 dB for 30 minutes to 1 hour and 30 minutes and a sound level of 80 dB for 1 hour at 800 Hz, It is not limited.

In general, the salt concentration of the soil capable of cultivating crops is 0.3% or less, and the rice is generally cultivated at a salt concentration of 0.2% or less, and the salt stress is preferably 0.2-1.6% in the soil, but is not limited thereto.

The plants include root crops including radish, turnip, cabbage, cabbage, carrot, burdock, sweet potato, horse root, lotus root, potato and ginger; Medicinal plants including ginseng, 칡, and udon; Forage crops including corn and soybeans; Dominant crops including rice; And oilseed rape, and rice is particularly preferable, but is not limited thereto.

In addition, the present invention provides a method for promoting seed germination under salt stress conditions using sonic waves, which comprises treating a plant seed with a sonic wave of 500 Hz to 1000 Hz.

It is preferable that the sound wave process is performed at a sound level of 50 to 100 dB for 30 minutes to 1 hour and 30 minutes and a sound level of 80 dB for 1 hour at 800 Hz, It is not limited.

In general, the salt concentration of the soil capable of cultivating the crop is 0.3% or less, and the rice is generally cultivated at a salt concentration of 0.2% or less, and the salt stress is preferably 0.2 to 1.6%, but not limited thereto.

In a specific embodiment of the present invention, the inventors treated specific sonic waves with rice seedlings and applied salt stress to sonicated seedlings. As a result, the growth of rice seedlings treated with a specific sound wave was better than that of the control group not treated with sound waves. In addition, the weight, length and moisture content of the underground of the rice seedlings treated with sonication were higher than those of the control group, and water and nutrients were well ingested. The relative moisture content and the maximum chlorophyll fluorescence value (Fv / Fm ) Were higher than those of the control group, indicating that the rice seedlings treated with sonication were resistant to salt stress by confirming that water availability and photosynthetic mechanism damage of the leaves were small. In addition, the germination rate of rice seeds treated with sonic waves was higher than that of the control group under salt stress conditions, suggesting that the sonication improves seed germination efficiency by overcoming salt stress which affects seed germination.

Therefore, by using the method of enhancing the resistance under salt stress using the sound wave of the present invention and the seed germination promoting method, the plant can be easily cultivated in a high salt concentration environment, which is a poor environment in which growth and germination are difficult.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more fully explain the present invention to those skilled in the art.

< Example  1> sonicated rice Seedlings  Ready

<1-1> Rice Seedlings  Ready

DONGJIN seeds (produced in 2012 by the Korean Academy of Agricultural Sciences) were immersed in 1% Spotsack (Dongbu Hanon Co., Ltd.) for 24 hours and then washed 3 to 4 times in running water. The washed seeds were immersed in clean water for 2 days and germinated. The germinated seedlings were grown in Yoshida solution (1.43 mM NH ₄ NO ₃ , 0.37 mM NaH ₂ PO ₄ .H ₂ O., 0.51 mM K ₂ SO ₄ , 1 mM CaCl ₂ , 1.6 mM MgSO ₄ .H ₂ O, 0.44 mM Iron chelate, 0.01 mM MnCl 2 · H 2 O, 1.5x10 -4 mM (NH 4) 6Mo 7 · O 2 · H 2 O, 0.02 mM H 3 BO 3, 3x10 -4 ZnSO 4 · H 2 O, 1.6 × 10 ^-4 CuSO ₄ · H ₂ O, 0.071 mM citric acid, pH 6.0) until the third seedlings were grown.

<1-2> Sound wave processing

The seeds of the three-leaf rice cultivated in Example <1-1> were irradiated with 800 Hz sound waves for 1 hour using a noise-free chamber (Soundless chamber, manufactured by Korea Science and Technology Corporation) dB) is 80dB.

< Example  2> The sonicated rice in salt stress condition Seedling  Growth confirmation

In order to observe the resistance to salt stress in the rice seedlings by sonication, the rice seedlings treated in the above Example 1 were placed on the silent growth for 6 hours after the sonication, and the Yoshida solution ), 75mM, 150mM and 225mM NaCl were added, and the growth state of the seedlings was observed while culturing for 3 ~ 4 days.

As a result, the addition of 75 mM NaCl did not show any significant difference from the control, but at the concentration of 150 mM NaCl, the rice seedlings of the control without treatment of the sound wave had water absorption trouble at the root, , But the sonatized seedlings showed normal leaf growth. In the case of high concentration of 225 mM NaCl, both the rice treated with sonication and the untreated control were inhibited from the total growth due to drying of the leaves, but it was confirmed that the sonication treated with sonication was less salty than the control (Fig. 1 ).

Therefore, considering that the salt concentration of the soil in which the crop is generally cultivable is 0.3% or less and the rice cultivated at a salt concentration of 0.2% or less is considered, the sonicated rice seedlings of the present invention exhibit a high salt of 150 mM (0.8% , It can be seen that the sound wave treatment increases the resistance to salt stress.

< Example  3> The sonicated rice in salt stress condition Seedling  Root investigation

<3-1> Sound-treated rice in salt stress condition Seedling  Root Fresh  Confirm

The effects of high salt concentration on the rooting of the salt stress were investigated because water and nutrients were not absorbed from the soil to inhibit plant growth.

Specifically, rice seedlings treated with sonication in the above <Example 1> were placed on silent growth for 6 hours after the sonication, and Yoshida solution (control group) without salt treatment, Yoshida solution with 75 mM and 150 mM NaCl After cultivation for 3 days, rice cultivated under various conditions was collected and weighed (fresh) was measured.

As a result, it was confirmed that roots of rice seedlings treated with sound waves were heavy in the freshness of roots of various individuals under each condition (FIG. 2).

<3-2> Sound-treated rice in salt stress condition Seedling  Check root length

High salt concentration inhibited the growth of plants, because water and nutrients were not properly absorbed from the soil, so we investigated the effects of sound waves on salt stress on root growth.

Specifically, rice seedlings treated with sonication in the above <Example 1> were placed on silent growth for 6 hours after the sonication, and then cultured in Yoshida solution (control group) not treated with salt and Yoshida solution containing 150 mM NaCl for 3 days After culturing, the cultivated rice was cultivated under various conditions and the root length was measured.

In addition, the growth of roots showed a tendency to be thinner and thinner as the salt concentration increased. However, the rice seedlings treated with sonication had a higher number of roots than those of the control at 150 mM NaCl concentration, And long growing condition (Figs. 3 and 4).

< Example  4> The sonicated rice in salt stress condition Seedling  Check moisture content

<4-1> Sound-treated rice under salt stress condition Seedlings  Determine the moisture content of roots

In the <Example 3>, it was observed that the growth condition of the roots by the sonic treatment was better than that in the control under the salt stress condition. Therefore, the rice seedlings treated in the <Example 1> (Control), Yoshida solution containing 75 mM and 150 mM NaCl, and incubated for 3 days. The moisture content of the roots was determined.

As a result, it was confirmed that 12.4% and 12.4% of water were contained at a salt concentration of 75 mM NaCl and 12 mM NaCl, respectively (FIG. 5).

Therefore, it was found that the sonic treatment had a positive effect on the growth of not only the ground but also the underground part under the salt stress condition, so that the crops could maintain the normal growth condition even when exposed to poor environmental conditions.

&Lt; 4-2 > Sound-treated rice in salt stress condition Seedlings  Determine the relative moisture content of leaves

Relative moisture content of rice seedlings was investigated in terms of physiological photochemical reaction to salt stress.

Specifically, rice seedlings treated with sonication in the above <Example 1> were placed on silent growth for 6 hours after the sonication, and Yoshida solution (control group) without salt treatment, Yoshida solution with 75 mM and 150 mM NaCl After 3 days of transplanting, fresh weight (FW) was measured by cutting leaves of seedlings to 4 cm from the end of leaves to the same size. After measuring the fresh weight, the leaves were collected again and immersed in a Petri dish containing distilled water for 4 hours to absorb enough water, and the turgid weight (TW) was measured. After the pressure was measured, the leaf was placed in an envelope and dried at 70 ° C. for 24 hours. Dry weight (DW) was measured in the building, and the relative moisture content Respectively.

^* W: Fresh weight, TW: Turgid weight, DW: Dry weight,

As a result, when rice seedlings were exposed to salt, they showed signs of impairment of plant appearance, but in the case of rice seedlings treated with sonication, the growth of leaves was better than that of the control group. In addition, the relative moisture content of leaves was 3.8% higher at 75 mM NaCl and 15.2% higher at 150 mM NaCl concentration than the control (Fig. 6).

< Example  5> Sound-treated rice in salt stress condition Seedling  Maximum chlorophyll fluorescence ( Fv / Fm ) Measure

The rice seedlings treated with sonication in the above Example 1 were placed in a no-noise condition for 6 hours after the sonication, and then transferred to a Yoshida solution to which no salt was treated (control group), 75 mM and 150 mM NaCl, And after 3 days, chlorophyll fluorescence was confirmed using a fluorescecse parameter at 2/3 of the leaves. The maximum quantum yield (Fv / Fm) for the photochemical reaction when the photosynthesis of the plant leaves was maximized was calculated by using the following equation (2) as the initial fluorescence value and the maximum fluorescence value measured by the fluorescence meter.

^* F ₀ : initial fluorescence value and Fm: maximum fluorescence value

As a result, the maximum quantum yield (Fv / Fm) showing a low stress level was 62.5% higher in the rice treated with the 150 mM NaCl (0.8% salt) than the control (Fig. In the case of healthy higher plants, the Fv / Fm value of the leaves is maintained at 0.8, while the lower value is the case when the photosystem is damaged or exposed to the stress environment. Therefore, You can see that you have received less.

< Example  6> The sonicated rice in salt stress condition Seedling  Germination rate survey

The germination rate of rice seeds was investigated in order to observe the effects of sonication under salt stress conditions on rice seed germination.

Specifically, the filter paper was placed on a petri dish, 3 ml of distilled water was added, and a sterilized rice seed surface was treated with a 1% SPOT, and a 800 Hz sound wave was treated at 80 dB for 1 hour. The rice seeds treated with sonication were placed on a filter paper with Yoshida solution (control), 75 mM and 150 mM NaCl added, and germinated for 3 days in a Petri dish to investigate the germination rate of the seeds .

As a result, the germination rate of seeds was 6% higher than that of the untreated control, 5.8% at 75 mM NaCl and 18.5% at 150 mM NaCl (Table 1).

Germination rate (%) Salt concentration  ( mM ) 0 75 150 Control group 94.34 80.77 62 Sonication group 100 85.45 73.47

Claims (5)

A method for enhancing salt stress resistance of a plant using a sound wave, the method comprising treating a plant with a sound wave of 500 Hz to 1000 Hz.
[2] The method of claim 1, wherein the sound wave process is a process of processing a sound wave of 700 Hz to 900 Hz at a sound level of 50 to 100 dB for 30 minutes to 1 hour and 30 minutes.
The method of claim 1, wherein the salt stress is a salt concentration of the soil of 0.2 to 1.6%.
The plant according to claim 1, wherein the plant is a root crop plant including radish, turnip, cabbage, cabbage, carrot, burdock, sweet potato, horse root, rootstock, potato and ginger; Medicinal plants including ginseng, 칡, and udon; Forage crops including corn and soybeans; Dominant crops including rice; And oilseed crops including oilseed rape. The method of enhancing the salt stress resistance of a plant using a sound wave.
A method of promoting seed germination under salt stress conditions using sonic waves, comprising treating a plant seed with a sonic wave of 500 Hz to 1000 Hz.

Images