KR101250491B1 - Method for growing plants - Google Patents

Abstract

A plant cultivation method is disclosed. According to an aspect of the present invention, a plant cultivation method in which a speaker having a frequency selected from a frequency range of 1 Hz to 3 KHz and generating sound waves having a sound pressure of 65 to 85 dB generates the sound waves to the plant to grow the plant, It is possible to prevent the growth and pests on plants by using sound frequency and single ultrasonic frequency with specific sound pressure, and to promote the growth of crops and prevent pests without noise pollution, environmental pollution and ecosystem destruction. In addition, by controlling the expression of a specific gene possessed by the plant by the sonic treatment of a specific sound range, by treating a specific single sound wave to the target crop, there is an effect that can control the gray mold disease.

Plant, sound waves, ultrasound, frequency, sound pressure.

Description

Method for growing plants

The present invention relates to a method of plant cultivation.

The method of promoting the growth by bringing music to animals and plants, especially crops, has the advantage of promoting the growth of crops, preventing pests, and causing no environmental pollution, unlike growth methods using chemicals such as fertilizers and pesticides. have.

Attempts to promote growth by bringing music to plants were first attempted by Charles Darwin, who claimed evolution in circa 1860, and in 1950, Indian professor Singh gave the Indian traditional music, Lagar, to rice, peanuts, and tobacco to increase yields. It is known that this is due to sound waves resonating to plant cells, stimulating metabolism, and acoustic energy promoting the movement of cell molecules and plasma.

However, the music used in the conventional growth promotion method mainly uses high frequency of 4,000Hz or more and at the same time uses complex music similar to the symphony playing music by the orchestra, so that it stimulates the human auditory nerve, causing stress, and listening to the artificial sound. In the early period of time, it was also a problem that the grower's work efficiency decreased and the plant growth efficiency decreased. In addition, according to the prior art had a problem that can not be economically grown plants when using the electric method to promote the growth of plants.

In addition, according to the prior art, the method and apparatus for the growth and suppression of diseases and pests of copper, plants and the like using sound waves, ultrasonic waves, etc. simply have the effect that the music, sound waves, ultrasonic waves, etc. The main content of this study was to provide a wide range of frequency ranges without presenting the method and system implementation of the method in the wider crop cultivation space and the narrow and long facility house. Therefore, according to the prior art, there is a problem that a practical and effective method is not presented in the actual agricultural plantation farm.

The background art described above is technical information possessed by the inventors for the derivation of the present invention or acquired during the derivation process of the present invention, and is not necessarily a publicly known technique disclosed to the general public before the application of the present invention.

The present invention is beneficial to plants, by using a sound wave frequency and a single ultrasonic frequency having a certain sound pressure to prevent growth and pests on the plant, promote the growth of crops and prevent pests without noise pollution, environmental pollution and ecosystem destruction Provides a plant growing method that can be.

In addition, the present invention provides a plant cultivation method for regulating the expression of a specific gene possessed by a plant by sonic treatment of a specific band.

In addition, the present invention provides a plant cultivation method that can control gray mold by treating a specific single sound wave to the target crop.

Technical problems other than the present invention will be easily understood through the following description.

According to an aspect of the present invention, there is provided a plant cultivation method in which a speaker having a frequency selected from a frequency range of 1 Hz to 3 KHz and generating a sound wave having a sound pressure of 65 to 85 dB generates the sound wave to the plant to grow the plant. .

Here, the speaker has a frequency selected from the frequency range of 20KHz to 26KHz, it is possible to further generate an ultrasonic wave having a sound pressure of 65 to 85dB.

Here, the sound wave has a frequency selected from the frequency range of 1Hz to 3KHz, the first sound wave having a sound pressure of 65 to 85dB, and has a frequency selected from the frequency range of 1Hz to 3KHz, different from the frequency of the first sound wave, 65 to It may include a second sound wave having a sound pressure of 85dB.

Here, the speaker may be installed in a facility house, the speaker is a plurality, the separation distance of the adjacent speaker may be 40m to 60m.

In addition, the present embodiment can promote the growth of the plant by generating the sound waves for 2 to 4 hours per day to the plant.

Other aspects, features, and advantages other than those described above will become apparent from the following drawings, claims, and detailed description of the invention.

Plant cultivation method according to the present invention is beneficial to plants, by using a sound wave frequency and a single ultrasonic frequency having a certain sound pressure to prevent growth and pests on the plant, and the growth of crops without noise pollution, environmental pollution and ecosystem destruction It has the effect of promoting and preventing pests.

In addition, the plant cultivation method according to the present invention controls the expression of a specific gene possessed by the plant by sound wave treatment of a specific range, and by treating a specific single sound wave to the target crop, there is an effect that can control the gray mold disease.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. However, this is not intended to limit the present invention to specific embodiments, it should be understood to include all changes, equivalents, and substitutes included in the spirit and scope of the present invention.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

In the following description of the present invention with reference to the accompanying drawings, the same components are denoted by the same reference numerals regardless of the reference numerals, and redundant explanations thereof will be omitted. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

1 is a view of a plant cultivation apparatus according to an embodiment of the present invention. Referring to FIG. 1, the wave generator 110 generates sound waves and / or ultrasonic waves, which are transmitted to the plant through the speaker a.

Here, the sound wave may have a frequency of 1 Hz-3 KHz, and the frequency of the ultrasonic wave may be 23 KHz (+ / _ 3 KHz). In addition, the sound pressure (dB) of sound waves and ultrasonic waves can be any sound pressure of 65-85 (dB) in size suitable for a large crop growing area and a narrow and long facility house facility. The sound pressure of the ultrasonic wave and the sound wave generated in the speaker a is 85 (dB), and at about 35 m point (c), the sound pressure is reduced to 65 (dB). In addition, at the maximum reach distance b, the magnitude thereof is measured weakly.

In addition, the part drawn with dotted line (up, down 10m / left, right 40m) is the sound wave and ultrasonic sound pressure area, and it can measure sound pressure of about 65 (dB) at the edge.

In the case of the rear end d of about 4 m from the speaker a, a sound pressure of about 65 dB is generated. The speaker (a) is in the right direction and the sound pressure is adjusted by designing the speaker body structure in the left direction.

With this structure, it is possible to create a plant cultivation environment through the control of the frequency and sound pressure of sound waves and ultrasonic waves.

2 is a view showing one plant cultivation apparatus installed in a facility house according to an embodiment of the present invention. Here, the facility house (e) is a type 1-2W (600 pyeong) facility house is currently being recommended for distribution.

  The ranges d, b, c of sound waves and ultrasonic waves generated in the speaker a in the facility house e are generated corresponding to the sound pressure (65-85 dB) as shown. Thus, for even distribution of sound pressure in the narrow and long facility house (e), eight speakers (a) connected to the wave generator 210 are arranged to give the crop the appropriate sound pressure ((dB)) in the wide facility house (e). Can be.

A structure in which eight speakers 2, 12, 13, 14, 15, 16, 17, and 18 are connected to the wave generator 210 is illustrated in FIG. 3A. Referring to FIG. 3A, eight loudspeakers 2, 12, 13, 14, 15, and 16 are provided inside the facility house 1 with regions 5, 7, 8, and 9 of the sound waves and ultrasonic waves described above in FIG. 1. , 17, 18) are installed. In the case of the sound pressure described above, the speaker looking at each other is installed spaced 40m to 60m apart.

In order to affect a large facility house 1 with a single speaker, its output is very large, causing noise damage due to the sound waves output outside the facility house 1 rather than the sound waves acting inside the facility house 1. The arrangement of speakers 2, 12, 13, 14, 15, 16, 17, 18 in the facility house 1 is required.

In addition, in the case of a facility house having a larger area than the 1-2W type described above, the speaker may be installed according to the area formed by the sound wave and ultrasonic lines. The speaker 12 and the speaker 13 are arranged at intervals of 4-5 m in opposite directions.

Figure 3b is a photograph showing a comparison result of plants grown by treatment for 2 to 4 hours a day by the plant growing apparatus according to an embodiment of the present invention. (C-1) is a plant not treated with sound waves, (B-1) is a plant treated with sound waves of 40 to 60 dB, and (A-1) is a plant treated with sound waves of 65 to 85 dB. The plant to be treated is cucumber, and the treatment period is 20 to 25 days. Sound waves according to the present embodiment can be continuously processed during the growing period of the target plant. Referring to Figure 3b, it can be seen that the growth rate and size of the plant increases when treated with sound waves of 65 to 85dB.

The sound wave according to the present embodiment may be a single sound wave or a complex sound wave. In the latter case, the sound wave has a frequency selected from a frequency range of 1 Hz to 3 KHz, a first sound wave having a sound pressure of 65 to 85 dB, a frequency selected from a frequency range of 1 Hz to 3 KHz, and is different from a frequency of the first sound wave, It may include a second sound wave having a sound pressure of 65 to 85dB. Here, the first sound wave and the second sound wave may have the effect of suppressing specific gene expression and gray mold, as described below.

In addition, according to another embodiment of the present invention, after treating the rice with a compound sound wave and has an aldose gene showing a significant difference in expression of a number of genes selected by unprocessed rice and the Reverse Northern method As a result of the experiment, the amount of expression may be reduced or amplified by the variation of the sound wave and the processing time of a specific frequency processed externally. In addition, the promoter of this gene was isolated, fused with a marker gene, transformed into rice, and the same treatment was performed on the transformed rice. As a result, the promoter sensed an external specific sound wave and expressed the expression of another gene associated with it. It can be adjusted in the same form. This embodiment is disclosed in Republic of Korea Patent No. 10-0716616 as described below.

According to this embodiment, the plant is known to control the growth and control the external environment by controlling the expression of various genes owned by the present invention, the various genes possessed by the plant when processing a particular sound wave at the desired time through the present invention By artificially expressing them, it is possible to induce short-term self-defense mechanisms against growth regulation or disease and poor environment of the plant.

In addition, when the promoter of the isolated aldose combined with the desired gene and then introduced into the plant, it is possible to amplify the expression of the introduced gene by a specific sonication in real time. For example, disease and poor environmental disasters are the biggest cause of crop yield reduction (more than 50%), which is a decisive factor in crop cultivation limits. Recently, molecular biology research has revealed the function of crop genes, and it has been reported that disaster resistance is increased or plant growth and physiological metabolism can be controlled by controlling the expression of these genes. When applied, it is possible to induce disaster resistance of artificial crops, control plant growth and metabolism by controlling expression of plant genes in real time.

The present embodiment relates to a method for artificially regulating the expression of a specific gene possessed by rice by specific sonication, and includes the following steps. That is, the present embodiment (A) a subtractive screening for selecting a rice gene showing a difference in expression by the complex sound waves, (B) confirming the expression difference by the compound sound waves for each of the selected genes Step (C) confirming the result that the Aldoraz and Rubisco genes, which are genes that respond to light among the selected genes, react in the dark state with the complex state by the complex sound wave, (D) specific sound wave It may include the step of processing a specific sound wave on the noiseless plant growth specially manufactured to process (E) and the step of demonstrating that the expression level of the aldose gene is controlled by the processing and processing time for each sound wave of a specific frequency.

In addition, according to the present embodiment, the promoter of the aldose gene, which is a gene that responds to a specific sound wave, was isolated, and its function was determined. This process may include the following steps. That is, in the present embodiment, (F) isolation of the promoter of the aldose gene and binding to the GUS gene, which is a marker gene, to produce a carrier for plant transformation, (G) infecting Agrobacterium on rice malt callus Transforming and re-differentiating to cultivate, (H) confirming and selecting the introduction of the gene by PCR method using a bar specific primer, (I) whether to express the GUS gene by RT-PCR method using the GUS specific primer (J) confirming whether the aldose promoter introduced from the transformed rice maintains its original properties and expressing the photoreaction, and (K) specific sound waves in the transformed rice (50 and 250 Hz). ) After each treatment may include determining whether the GUS gene is regulated by RT-PCR using a GUS specific primer.

There are two types of sound waves used in this embodiment, the first being a compound sound wave, which is a general CD composed of various classical music such as Mozart's fate symphony and Vivaldi's four seasons. The plant was told through. The CD contains a wide variety of complex sounds, making it difficult to determine which specific sound waves regulate gene expression.

The second is a specific sound wave, which was processed using a computer software program called a computer sound generator produced by the Korea Advanced Institute of Science and Technology. The program could be easily used to identify specific bands that are most effective in controlling gene expression by allowing computers to process the desired frequencies for the desired time through speakers mounted in the growing field.

According to this example, experiments were carried out using the ranges of 50, 125, 250, 1 and 5 KHz, but in conclusion, 250 Hz was the most effective range for the expression of the Aldolase gene and the regulation of its promoter. Using the opposite band, the opposite effect could induce or decrease the expression of the Aldorase gene.

The promoter of the aldose gene was selected and separated in consideration of its usefulness, universality and sensitivity of sonic response. Aldorase genes are essential genes for almost all organisms, from lower microorganisms to higher animals, and are essential to sustain life. In plants, they are important genes involved in starch synthesis processes. In addition, it is one of the genes most sensitive to sonic processing among a plurality of genes that respond to sound waves as a result of the above analysis. When this promoter is combined with other specific genes and introduced into the plant, it is possible to artificially regulate the expression of the specific gene associated with this promoter by processing specific sound waves, thereby controlling plant growth or disease and poor environment. The possibility of inducing short-term self-defense mechanisms against this could be realized.

Referring to FIG. 4, genes showing a difference in expression after a compound sonication treatment in rice are selected. In the growing phase, rice was treated with a compound sound wave, and a gene having a difference in expression was selected in response to the sound wave. A number of genes were selected by subtractive screening method by Northern hybridization analysis, and based on the results of analyzing the respective sequences and searching for homology, fructose 1,6-bisphosphate aldose Four genes were finally selected: Fructose 1,6-bisphosphate aldolase, DNA J, Rubisco, and Calreticulin.

Referring to Figure 5, the expression pattern of the genes by compound sonication is analyzed. In order to analyze the difference of expression by compound sound for each selected gene, three genes except the Calreticulin gene were cloned to make a probe with plasmid DNA, and using it, Northern hybridization. It was confirmed by the method. On the other hand, the caleticulin gene was prepared by 5'-ATGGGAAGTCGCAGCGGCGGC-3 'and 5'-TCACAAATGAGGATCTTCTCA-3' gene-specific primers, and denatured at 95 ° C for 5 minutes, and denatured at 94 ° C for 1 minute, 55 The cycle of annealing at 1 ° C. for 1 minute and expanding at 2 ° C. at 72 ° C. was repeated for 30 times, and finally, it was analyzed by reacting at 72 ° C. for 10 minutes. As described above, the sound waves were processed using a general CD composed of various music such as classical music, that is, Mozart's fate symphony and Vivaldi's four seasons, and the classical music contained in the CD was grown for four hours using a computer. The rice plants were treated. From the results of FIG. 5, the expression of fructose 1,6-bisphosphate aldolase, Rubisco, DNA J and calreticulin genes was untreated during sonication. It can be seen that the increase significantly compared to.

Referring to Figure 6, the expression pattern of the photoreaction genes by compound sonication is analyzed. Among the genes isolated from above, the Aldolase gene and the Rubisco gene are genes whose expression is controlled by light. In order to analyze whether these genes can induce the expression of these genes even in the cancerous state by sound waves, sound waves in the same way as rice grown in the light state after growing three leafy rice for 10 days in the cancerous state Was treated to analyze the expression pattern. Expression was performed by Northern blot (Northern blot) method, the results are shown in FIG. Through the results of FIG. 6, it was confirmed that the expression of the aldose gene was increased during complex sonication in the cancer state.

Referring to Figures 7 and 8, the expression pattern of the aldose gene by time-specific processing according to a specific frequency and frequency in the noise-free growth is analyzed. A signal generator was used to treat 50, 125, 250, 500, 1k and 5 KHz for 4 hours each. Each frequency-treated rice leaf was harvested separately, RNA was isolated, and the change of expression level by sound treatment was analyzed by Northern blot. As shown in the results of analyzing the expression patterns of each frequency treatment, the expression level was decreased at 50 Hz, whereas the expression level was increased at 250 Hz. Looking at the Northern blot results, the expression level was slightly decreased compared to the no treatment at 50 Hz, whereas the expression level was significantly increased at 250 Hz. On the other hand, when the 50 Hz and 250 Hz treatment, the analysis of the expression pattern according to the hourly treatment, the gene expression was increased between 1 hour and 2 hours at 50 Hz treatment, and gradually decreased after 4 hours after treatment Could see. On the other hand, in the 250 Hz treatment, the expression level started to increase after 30 minutes of treatment, and the expression level reached after 1 hour, and the increased expression level was maintained until 4 hours, and then gradually decreased.

In addition, according to another embodiment of the present invention, there is provided a cultivation method for inhibiting the growth of gray fungus (Botrytis cinerea). This embodiment is disclosed in Korean Patent Registration No. 10-0795421.

In the present invention, the single sound wave processing uses a sound wave generator. The sound wave generator is not necessarily limited thereto, and refers to a computer in which a computer software program (manufactured by the Korea Advanced Institute of Science and Technology), which is called a sound generator, is embedded, and the single sound wave processing step specifically includes a sound wave generator and a growth image. It refers to the process of processing a single sound wave to fungus hyphae or crop by connecting the speaker installed inside. The computer software program is a program designed to select and process a single sound wave of a desired sound range by dividing it into fine sound ranges. In order to exclude the influence of sound waves other than the sound wave processed during the processing of the sound waves, the growth phase was specially made so that the noise itself was little and was named as the noiseless growth phase.

In the present invention, the target crop refers to all crops that can be infected with ash fungus by Botrytis cinerea, and specifically, vegetable crops such as strawberries, lettuce, cucumbers, eggplants, peppers, lilies, gladiolus, and roses. The same flower crop.

According to the present embodiment, as a result of processing a single single sound wave selected at a frequency of 50 Hz to 5 KHz to Escherichia cloi to identify a condition for inhibiting the growth of a specific microorganism using sound waves, Showed different results. Referring to FIG. 9, that is, even when 50 or 500 Hz was treated, the number of colonies was decreased, but when the single sound waves of 125 Hz and 250 Hz were treated, the number of colonies of bacteria was significantly reduced compared to the untreated group. Could observe. According to this, the frequency of a single sound wave that suppresses the growth of a particular microorganism has an appropriate range.

In an embodiment of the present invention, as a result of treating a specific single sound wave selected from the frequency 50 Hz to 5 KHz to the gray mold fungus based on the above results, the growth and spore formation rate of the hyphae when the frequency 250 Hz and 500 Hz single sound wave were treated. The results were found to be significantly reduced compared to this untreated group. However, when processing 50 Hz, 125 Hz or 1 KHz sound waves, the difference is not remarkable than that of 250 Hz or 500 Hz sound waves, indicating that the specific single sound wave affecting growth differs depending on the type of microorganism. Could.

In addition, referring to Figure 16, in order to investigate the effect of the monosonic wave at the frequency of inhibiting the growth of the fungus (Botrytis cinerea) on the spore formation of the next generation, the flora derived from the mycelium treated with 500 Hz monosonic wave When transferred to a new medium and incubated without secondary sonication, it was observed that the sonic treated in the previous generation also affected the sporulation of the next generation and markedly suppressed sporulation.

Therefore, the present embodiment provides a method of suppressing the growth of gray fungus bacteria by treating a target crop with a single sound wave selected from sound waves having a frequency of 250 Hz to 500 Hz.

In another embodiment of the present invention by treating the leaves of cucumber inoculated with spores of ash fungus bacteria 500 Hz single sound wave to investigate whether the single sound waves inhibit the development of cucumber ash fungal disease. As a result, it was confirmed that there is a control effect of about 44% to 85%. Accordingly, the present invention provides a method for controlling gray mold disease by treating a single crop with a single sound wave selected from sound waves having a frequency of 250 Hz to 500 Hz.

Referring to FIG. 9, the effect of sound waves on the colony number of Escherichia coli is shown. Escherichia cloi was treated with a specific single sound wave selected at a frequency of 50 Hz to 5 KHz to determine the conditions for inhibiting the growth of specific microorganisms using sound waves. In order to accurately measure the effect of a specific single sonication, sonication was processed in a special custom noiseless plant growth. The growth phase is about 35-40dB of self-generated noise, and the environment in the growth phase is made to remain almost silent, which is useful for measuring the effects of specific sound waves.

LB solid medium (Luria Bertani: bacto-tryptone 10 g, bacto-yeast extract 5 g, NaCl 10 g and agar 15 g / L) with a suspension of bacteria (1 × ^{10 8} cfu / mL) smeared in the noiseless growth phase equipped with a speaker Was dissolved in 950 ml of distilled water, then added about 200 μl of 5 N NaOH to adjust the pH to 7, filled to 1 liter with distilled water, sterilized and placed in a chalet and placed into a solidified medium. , Using the Korea Advanced Institute of Science and Technology), and treated the single sound wave by frequency (50Hz, 125 Hz, 250 Hz, 500 Hz, 1 KHz) for 2 hours, transfer the solid medium to the growth at 37 ℃ for 24 hours The colony number of the bacteria was compared with the colony number of the medium not treated with sound waves, and the results are shown in FIG. 9. As shown in Figure 9, compared with the control group, the number of colonies was significantly reduced in the medium treated with sound waves, in particular, the medium treated with a single sound wave of 125 and 250 Hz.

The effects of sound waves on Botrytis cinerea are as follows. It is assumed that the treatment conditions of the sound waves that inhibit growth according to the type of microorganism of interest are selected from 50 Hz to 5 KHz to search for the frequency of the single sound waves that inhibit the growth of Botrytis cinerea. Specific single sound waves were treated to observe spore-forming changes of mycobacterial fungi, morphological changes of mycelia and next-generation spore-forming changes of the pathogens.

The effects of sound waves on the spore formation of gray fungus bacteria are as follows. 6 mm of gray fungus bacteria were inoculated into potato extraction medium (Potato Dextrose Agar, PDA) using a cork bore (diameter 6 mm) in a noise-free growth box equipped with a speaker, and a sound generator (Korea Institute of Science and Technology) ), And then treated with single frequency wave (50 Hz, 125 Hz, 250 Hz, 500 Hz, 1 KHz) for 2 hours and transfer it to growth at 25 ℃ for 2 days, then incubate for 2 days Growth and spore formation rates were compared.

As a result, referring to Figure 10, when the sound waves were treated, especially in the single sound wave treatment of the frequency of 250 Hz and 500 Hz, the mycelial growth and spore formation rate was significantly reduced compared to the untreated group. Therefore, it can be seen that the most effective single sound wave that suppresses the growth of gray mold fungus is a single sound wave selected from the frequency of 250 Hz to 500 Hz.

In addition, in order to confirm whether the effect of suppressing sporulation by monosonic waves is repeatable and persistent, the 250 Hz and 500 Hz monosonic waves having significantly reduced spore formation are treated three times in the same manner as above, and the results are shown in FIG. 11. And FIG. 12, respectively. As shown in Fig. 11 and 12, when the single sound wave was treated as compared to the untreated group all spore formation was suppressed, it can be seen that the effect of the spore formation of the gray mold fungus by the single sound wave treatment is repeatable. .

Referring to FIG. 13, in order to compare the effect of spore formation according to sonication time, the total bacterial count of gray mold fungi treated with 500 Hz single sound for 0.5 hours, 1 hour, 2 hours, 4 hours and 8 hours was 5 days. After culturing, the spore formation was effectively inhibited at all treatment times regardless of treatment time, and the most effective treatment time was 2 hours. In conclusion, the treatment of the single sound wave was found to be an effective control method of gray mold disease by repeatedly and continuously inhibiting the spore formation of gray mold fungi.

The effects of sound waves on the change of mycelia of gray fungus bacteria are as follows. A total of 6 mm of bacterial fungus bacteria was inoculated into potato agar extraction medium (Potato Dextrose Agar, PDA) using a cork bore (6 mm in diameter), and treated with 500 Hz single sound for 1 hour, and then grown at 25 ° C. After culturing for 5 days, the morphological characteristics of fungal hyphae were observed at an enlarged magnification of 400 times with an optical microscope, and the results are shown in FIGS. 14 and 15.

As shown in FIG. 14, in the untreated group, the typical mycelia were observed, but in the mycelium treated with single sound waves, most of the mycelia were dissolved. And, as shown in Figure 15, in the sonic-treated mycelium can be observed that the distal end of the hyphae in a deformed form, empty or dissolved between the diaphragm and the diaphragm.

As a result, it was found that the single sound wave treatment of the present invention prevented mycelial growth of gray fungus bacteria and abnormally deformed mycelial growth and spore formation.

The effects of sound waves on the sporulation of the next generation of gray fungi are as follows. In order to check the effect of monosonic waves of the frequency inhibiting the growth of the gray mold fungus on the sporulation of the next generation, 500 Hz sound waves were treated hourly (0.5, 1, 2, 4, 8 hours) for 5 days. After incubation for 6 mm, the germ mycelium from the soaked mycelium and 6 mm from the untreated mycelium were inoculated into a new potato agar extraction medium using a cork bore (6 mm in diameter) and treated with additional sonication. After incubation for 3 days in a 25 ℃ incubator without comparison with each other the spores formation rate is shown in Figure 16 the results.

As shown in Fig. 16, spore formation was remarkably suppressed in the next generation in the case of the mycelium derived from the mycelia treated with the single sound wave of the present invention. However, the effect of sonication time was not clearly seen. From the above results, it can be seen that the treatment of the single sound wave of the present invention prevents spore formation in the next generation as well as spore formation in the next generation, thereby preventing secondary infection caused by sporic acid. .

In addition, the analysis of the sound effect of gray mold disease of sound waves is as follows. In order to confirm that the treatment of the single sound wave is an effective control method of gray mold disease of the actual target crop, spore suspension (1x106 spore / mL) of gray mold fungus was inoculated by spreading the leaves of cucumbers for 3 weeks and inoculated without noise. 500 Hz sound waves were processed hourly (0.5, 1, 2, 4, 8 hours) using a sound generator (Korea Advanced Institute of Science and Technology). After the sonic treatment, the temperature was 23-25 ℃ and the humidity was maintained at about 70% in a dark state incubation while inducing the onset and observed the development of the disease every day.

The occurrence of gray mold disease was confirmed from the second day after inoculation of pathogens, and the incidence area was significantly lower in the cucumber leaves treated with monosonic waves compared to the untreated group (FIG. 17). On the other hand, to determine the proper sonication time, the leaves of cucumber were treated with the untreated group two days after the 500 Hz sonication was treated hourly (0.5, 1, 2, 4, 8 hours) to induce the onset in the same manner as above. A comparison is shown in FIGS. 18 and 19. As a result, cucumber leaf treated with sound waves significantly suppressed the development of gray mold, but there was no significant difference according to the sound processing time. In addition, as a result of observing the leaves 4 days after inoculation to confirm whether the control effect of gray mold disease by the single sound wave treatment of the present invention is continuously maintained, the onset was found to be considerably advanced, the same as the result after 2 days. In comparison with the untreated group, it was observed that the onset was significantly suppressed (see FIGS. 20 and 21).

The same procedure as above for the case where the 500 Hz sound waves were treated to cucumber leaves by time (0.5, 1, 2, 4, 8 hours) and the 250 Hz sound waves were treated to cucumber leaves by the same time as above. When the area ratio was calculated and the control value was calculated from the calculated lesion area ratio, it was confirmed that the single sound wave treatment had a control effect of about 44-85%. Through this, it can be seen that the treatment of the single sound wave selected at the frequency of 250 Hz to 500 Hz is an effective control method of gray mold disease of the actual target crop.

Those skilled in the art will appreciate that various modifications and changes can be made in the present invention without departing from the spirit and scope of the invention as set forth in the claims below.

1 is a view of a plant cultivation apparatus according to an embodiment of the present invention.

2 is a view showing one plant cultivation apparatus installed in a facility house according to an embodiment of the present invention.

3A is a view illustrating a plurality of plant cultivation apparatuses installed in a facility house according to an embodiment of the present invention.

Figure 3b is a view showing a comparison result of the plants grown by the plant cultivation apparatus according to an embodiment of the present invention.

Figure 4 is a photograph showing the results of the subtractive screening (Subtractive screening) using the Reverse Northern technology for selecting a rice gene showing a difference in expression by complex sound waves.

FIG. 5 is a diagram showing a Northern blot result for confirming the difference in expression by a compound wave for each of the selected genes. FIG.

Figure 6 is a photo showing the results of increased expression by the complex sound wave photoreaction genes Aldoreise and Rubisco gene even in the cancer state.

7 is a diagram showing a Northern blot result performed to analyze the expression variation of the Aldolase gene by sonic processing at a specific frequency.

8 is a view showing the northern blot results performed to analyze the variation in the amount of expression of the Aldolase gene by processing the sound waves of a specific frequency with time.

Figure 9 is a photograph showing the survival rate of bacteria 24 hours after incubation after treatment with a specific single sound wave after plating the suspension of bacteria (Escherichia coli) in a solid medium.

Figure 10 is a photograph showing the results of comparing the total growth and spore formation rate of the fungus after a two-hour treatment of B. cinerea with a variety of specific monosound and B. cinerea incubated for 2 days.

FIG. 11 is a photograph showing the results of comparing the total growth and sporulation rate after treating a single specific sound wave 250 Hz for 2 hours and incubating the treated mycelia for 2 days. FIG.

12 is a photograph showing the results of comparing the total growth and sporulation rate after treatment with a single specific sound wave 500 Hz for 2 hours and incubated the treated mycelia for 2 days.

Figure 13 is a photograph showing the results of comparing the total growth and sporulation rate after treatment with a single specific sound wave 500 Hz and incubated treated hyphae for 5 days.

FIG. 14 is a photograph of magnified mycelia at a ratio of 400 times the optical microscope after 5 days of incubation with a single specific sonication 500 Hz for 1 hour after inoculation to gray agar fungus bacterial medium.

FIG. 15 is a photograph of an image of the terminal area of the hyphae magnified at a ratio of 400 times the optical microscope after 5 days of incubation with a single specific sound wave 500 Hz for 1 hour after inoculation of the ash fungus bacteria in agar agar solid medium.

Fig. 16 shows the incubation rate of spore formation and spore formation after incubation for 5 days after incubating for 5 days with a single specific sonic wave 500 Hz, incubating for 3 days after removing the flora from the end of this mycelium. Photo showing results.

Fig. 17 shows the incidence area after incubating for 2 hours with a single specific sound wave 500 Hz for 2 hours on cucumber leaves inoculated with cucumber leaf spore suspension (1X10 ⁶ spores / mL) Photo showing comparison with treatment group.

Figure 18 is a photograph showing the area of incidence after incubation for 2 days after different treatment times of a single specific sound wave 500 Hz on the leaves of cucumber spore suspension (1X10 ⁶ spores / mL) of gray mold pathogens.

19 is a photograph showing details of a leaf in FIG. 18.

20 is inoculated with the spore suspension (1X10 ⁶ spores / mL) of the gray fungal pathogen to the leaves of cucumber and treated with a single specific sound wave 500 Hz at different treatment times and wet conditions for 4 days under conditions easy to induce the onset Results show the onset of untreated and sonicated leaves after treatment.

FIG. 21 is a photograph showing details of leaves in FIG. 20; FIG.

Claims (6)

A speaker having a frequency selected from a frequency range of 1 Hz to 3 KHz and generating sound waves having a sound pressure of 65 to 85 dB generates a sound wave to the plant and grows the plant. The sound wave is, A first sound wave having a frequency of 250 Hz for inducing aldose gene expression and having a sound pressure of 65 to 85 dB; And A plant cultivation method comprising a second sound wave having a frequency selected from a frequency range of 250 Hz to 500 Hz or less and suppressing the growth of gray mold, and having a sound pressure of 65 to 85 dB. The method of claim 1, The speaker has a frequency selected from the frequency range of 20KHz to 26KHz, and the plant cultivation method for generating an ultrasonic wave having a sound pressure of 65 to 85dB to the plant. delete The method of claim 1, And the speaker is installed in a facility house. 5. The method of claim 4, The speaker is a plurality, plant separation method, characterized in that the separation distance of the adjacent speaker is 40m to 60m. The method of claim 1, Plant cultivation method characterized in that for generating the sound waves for 2 to 4 hours per day to the plant.

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