CN112425405B - Application of ibuprofen in improving salt tolerance of plants - Google Patents
Application of ibuprofen in improving salt tolerance of plants Download PDFInfo
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- CN112425405B CN112425405B CN202011257582.3A CN202011257582A CN112425405B CN 112425405 B CN112425405 B CN 112425405B CN 202011257582 A CN202011257582 A CN 202011257582A CN 112425405 B CN112425405 B CN 112425405B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses application of ibuprofen in improving salt tolerance of plants. The ibuprofen has the capability of improving the salt stress resistance of plants, and is prepared into an ibuprofen solution with safe concentration and applied to plant seeds and seedlings subjected to salt stress by a method of soaking, irrigating or spraying. Under the condition of salt stress, ibuprofen can remarkably improve the germination rate of plant seeds, the survival rate of plant seedlings, the plant height, the root length, the bud length and the fresh weight, and the ibuprofen is simple to use, remarkable in effect, safe and reliable in plant planting, and very beneficial to plant growth and improvement of planting yield.
Description
Technical Field
The invention belongs to the field of plant planting, and particularly relates to application of ibuprofen in improvement of salt tolerance of plants.
Background
Soil salinization seriously affects the growth and quality of crops, so that the soil salinization becomes one of the main environmental factors limiting the yield of the crops at present. Various life activities of plants under salt stress are influenced by different degrees, and mainly appear in inhibiting the growth and development of the plants, destroying the cell structures of the plants, influencing the synthesis of biomolecules required for maintaining the normal physiological metabolism of the plants and the like. When the salt content in the soil is too much, physiological drought, toxic action of ions and normal metabolism damage to plants are usually caused. High salt ultimately leads to a reduction in crop economic yield by affecting aspects such as seed germination, seedling growth and vigor, plant flowering and fruiting, and the like. Efficient utilization of saline soil and improvement of crop salt tolerance have become issues of great concern.
Ibuprofen (Ibuprofen, IBU) is a derivative of phenylpropionic acid, and is a non-steroidal anti-inflammatory drug for relieving fever and pain. Ibuprofen is white crystalline powder; slightly peculiar smell, almost no smell. Ibuprofen has anti-inflammatory and analgesic effects by inhibiting cyclooxygenase to reduce prostaglandin synthesis, and has antipyretic effect by hypothalamic thermoregulation center. Ibuprofen has better effects of diminishing inflammation, resisting rheumatism, relieving fever and easing pain than aspirin, phenylbutazone and acetaminophen, and is clinically used for treating rheumatoid arthritis, rheumatic arthritis, ankylosing spondylitis, lupus erythematosus and the like and waist and back pain. In the field of botany, no patent and report that ibuprofen directly affects plant stress resistance is seen at present.
Disclosure of Invention
The invention aims to provide application of ibuprofen in improving salt tolerance of plants. The ibuprofen has the advantages that the salt stress resistance of plants is improved, and the seed germination rate, the plant height, the root length and the fresh weight of the plants under the salt stress can be obviously improved.
In order to realize the purpose of the invention, the invention is realized by adopting the following technical scheme:
the invention provides application of ibuprofen in improving salt tolerance of plants.
Further, the ibuprofen has the capacity of improving the salt stress resistance of plants.
Further, the chemical structural formula of the ibuprofen is as follows:
further, the application method of the ibuprofen comprises the following steps: preparing ibuprofen into ibuprofen solution, and culturing the plant seeds for 3-5 days at 25-30 deg.C with the ibuprofen solution.
Further, the ibuprofen solution is used in a volume of 0.2-0.4 mL/seed.
Further, the application method of the ibuprofen comprises the following steps: preparing ibuprofen into an ibuprofen solution, and then irrigating or spraying the ibuprofen solution to seedlings of plants in the seedling stage.
Further, the preparation method of the ibuprofen solution comprises the following steps: the ibuprofen is prepared into ibuprofen mother liquor by using absolute ethyl alcohol, and the ibuprofen mother liquor is diluted into the required concentration by using water.
Further, when the ibuprofen solution is used for irrigating seedlings of plants in the seedling stage, the irrigation volume of the ibuprofen solution is 50-100 mL/plant; after pouring, the ibuprofen solution is poured out after soaking for 12-14 hours each time, and the solution is poured once every 5-7 days and is continuously poured for 3-5 times.
Further, when the ibuprofen solution is used for spraying seedlings of plants in the seedling stage, the spraying volume of the ibuprofen solution is 0.3-1 mL/plant, and the ibuprofen solution is sprayed once every 4-5 days and is continuously sprayed for 5-6 times.
Further, the concentration of the ibuprofen solution is 10-500 mu M.
Further, the ibuprofen can improve the seed germination rate of plants under salt stress.
Further, the ibuprofen can improve the germination vigor of plants under salt stress.
Further, the ibuprofen can improve the seedling survival rate of plants under salt stress.
Further, the ibuprofen can increase the shoot length of the plant in the seedling stage under salt stress.
Further, the ibuprofen can increase the root length and fresh weight of plants under salt stress.
Further, the ibuprofen can increase the plant height of plants under salt stress.
Further, the salt concentration of the salt stress was 150-200 mM.
Further, the plants include millet, tobacco, wheat, sorghum and corn.
Compared with the prior art, the invention has the advantages and positive effects that:
ibuprofen is a plant jasmonic acid synthesis inhibitor and can play a role in plants. The invention proves that the ibuprofen has the capability of improving the salt tolerance of the millet for the first time, and the ibuprofen is prepared into the ibuprofen solution and is applied to the plant seeds and seedlings stressed by salt by using a soaking, irrigating or spraying method, so that the application of the ibuprofen solution can obviously improve the germination rate and the germination vigor of the plant seeds and reduce the salt damage under the condition of salt stress; can also improve the plant height, root length, bud length and fresh weight of the plant seedlings and the mature seedlings, thereby achieving the effects of protecting and strengthening the seedlings in production. The ibuprofen solution disclosed by the invention is safe in use concentration, free of side effects, free of damage to plants and accumulation in the plants, and further free of influence on the health of animals and human bodies eating the plants, so that the ibuprofen solution disclosed by the invention is simple to use, remarkable in effect, safe and reliable, and very beneficial to planting of the plants, and the planting benefit is improved.
Drawings
FIG. 1 is the effect of ibuprofen on the salt tolerance of millet seeds during germination;
FIG. 2 is the effect of ibuprofen on salt tolerance of millet seedlings;
FIG. 3 is the influence of exogenous ibuprofen spraying on salt tolerance of millet in the seedling stage;
FIG. 4 shows the effect of exogenous ibuprofen irrigation on salt tolerance of millet in seedling stage.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to the accompanying drawings and the detailed description. However, the present invention is not limited to the following embodiments, and the method is not specifically described as a conventional method. The starting materials are commercially available from the open literature unless otherwise specified.
Example 1: ibuprofen can promote germination under salt stress condition of millet
1. 20.628mg ibuprofen was prepared in 100mM stock solution in 1mL absolute ethanol, and then diluted with water to give 50. mu.M and 100. mu.M ibuprofen-treated solutions, respectively.
2. Selecting plump and healthy millet seeds, firstly disinfecting the surfaces of the seeds for 20 seconds by using 70% ethanol, and then cleaning the seeds for 5-6 times by using sterile water. Dividing the millet seeds into a blank group (CK), a control group and an experimental group, soaking the seeds of the CK group in clear water for 12 hours for pretreatment, and soaking the seeds of the control group and the experimental group in 150mM NaCl solution for 12 hours for pretreatment. Three groups of parallel experiments are arranged in each group, and 80 millet seeds are selected in parallel. Spreading the soaked seeds on a 9 cm glass culture dish which is placed with sterilized filter paper in advance, and culturing at 25 ℃; 5mL of water is added into the culture dish of the control group, 5mL of ibuprofen aqueous solution with the concentration of 50 mu M and 100 mu M is correspondingly added into each culture dish of the experimental group respectively, and after 3 days of culture, seed germination data are counted.
As can be seen from FIG. 1, compared with the control group, exogenous application of 50 μ M and 100 μ M ibuprofen can significantly increase the germination rate, fresh weight, bud length and root length of the millet seeds, and significantly promote the germination and growth of the millet seeds under salt stress; however, when the concentration of ibuprofen in water was increased to 500. mu.M, inhibition was produced.
Example 2: ibuprofen can improve salt tolerance of water-cultured millet seedlings
1. 20.628mg ibuprofen was prepared into 100mM stock solution with 1mL absolute ethanol, and then diluted with water to give ibuprofen treatment solutions of 10. mu.M, 50. mu.M, and 100. mu.M concentrations, respectively.
2. Selecting plump and healthy millet seeds, firstly disinfecting the surfaces of the seeds for 20 seconds by using 70% ethanol, and then cleaning the seeds for 5-6 times by using sterile water. After the millet germinates for 3 days, seedlings with consistent growth vigor are selected and laid on 9 cm glass culture dishes which are placed with sterilized filter paper in advance, 10 seedlings are placed in each dish, and the treatment liquid is 5 mL. Dividing into blank group (CK), control group and experimental group, wherein the seedlings of CK group are treated with clear water, the seedlings of control group are treated with 150mM NaCl solution, and the experimental group is respectively treated with 150mM NaCl containing 10 μ M, 50 μ M and 100 μ M ibuprofen aqueous solution; culturing at 25 deg.C; the phenotype was observed after 7 days.
As can be seen from fig. 2, compared with the control group, exogenous application of ibuprofen aqueous solution under the condition of salt stress can increase the plant height, root length and fresh weight of the millet seedlings, significantly promote the growth of the millet seedlings and improve the salt tolerance of the millet hydroponic seedlings.
Example 3: exogenous ibuprofen spraying can improve salt tolerance of millet in seedling stage
1. 20.628mg of ibuprofen was prepared into a 100mM stock solution using 1mL of absolute ethanol, and then diluted with water into 50. mu.M, 100. mu.M, and 500. mu.M aqueous ibuprofen solutions, respectively, as subsequent treatment solutions.
2. And (4) selecting full and healthy millet seeds. 15 millet seeds are planted in each black seed tray, 9 seedlings with consistent growth are selected after the millet sprouts for 3 days, and meanwhile, the rest seedlings are removed by tweezers. The 9 seedlings were treated after further growing for 3 days, and the seedlings in the CK group were watered with clear water, and the seedlings in the control group and the test group were watered with an equal amount of 200mM NaCl solution. Then, the seedlings of the CK group and the control group are sprayed with clear water, and the seedlings of the experimental group are respectively sprayed with ibuprofen aqueous solution of 50 mu M, 100 mu M and 500 mu M; the volume of the solution sprayed each time is 20mL, the spraying volume of each millet is ensured to be 0.3-1mL, so as to ensure that the leaves of the seedlings fully contact the liquid; spraying and fixing the fertilizer at 9 am every time, spraying the fertilizer once in 4 days, and counting related data after spraying the fertilizer 5 times.
As can be seen from fig. 3, compared with the control group, exogenous spraying of ibuprofen under the salt stress condition can significantly increase the plant height, root length and fresh weight of the millet seedlings, significantly promote the growth of the millet seedlings and improve the salt tolerance of the millet seedlings at the seedling stage.
Example 4: exogenous pouring ibuprofen can improve salt tolerance of millet in seedling stage
1. 20.628mg of ibuprofen was prepared into a 100mM stock solution using 1mL of absolute ethanol, and then diluted with water into 50. mu.M, 100. mu.M, and 500. mu.M aqueous ibuprofen solutions, respectively, as subsequent treatment solutions.
2. And (4) selecting full and healthy millet seeds. 15 millet seeds are planted in each black seed tray, 9 seedlings with consistent growth are selected after the millet sprouts for 3 days, and meanwhile, the rest seedlings are removed by tweezers. Treating the 9 seedlings after continuously growing for 3 days, irrigating the seedlings of the CK group by using clear water, irrigating the seedlings of the control group by using 200mM NaCl solution, and irrigating the seedlings of the experimental group by using 200mM NaCl containing 50 mu M, 100 mu M and 500 mu M ibuprofen aqueous solution respectively; 3L of pouring solution is quantitatively poured each time, the pouring volume of each plant is ensured to be 50-100mL, the plant is poured out after being soaked for 12 hours, the plant is poured once every 7 days, and data are counted after 3 times of pouring.
As can be seen from fig. 4, compared with the control group, exogenous irrigation of the ibuprofen-containing salt solution under the salt stress condition can significantly increase the plant height, root length and fresh weight of the millet seedlings, significantly promote the growth of the millet seedlings and improve the salt tolerance of the millet seedlings at the seedling stage.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (8)
1. The application of ibuprofen in improving the salt tolerance of plants is characterized in that the concentration of ibuprofen is 50-500 mu M; the plant includes millet, tobacco, wheat and sorghum.
2. The use of ibuprofen according to claim 1 for increasing salt tolerance in plants, wherein said ibuprofen is applied by: preparing ibuprofen into ibuprofen solution, and culturing the plant seeds for 3-5 days at 25-30 deg.C with the ibuprofen solution.
3. The use of ibuprofen according to claim 2 for increasing salt tolerance in plants, wherein the ibuprofen solution is used in a volume of 0.2-0.4mL per seed.
4. The use of ibuprofen according to claim 1 for increasing salt tolerance in plants, wherein said ibuprofen is applied by: preparing ibuprofen into an ibuprofen solution, and then irrigating or spraying the ibuprofen solution to seedlings of plants in the seedling stage.
5. The use of ibuprofen in improving the salt tolerance of plants according to claim 4, wherein the ibuprofen solution is poured in a volume of 50-100 mL/plant when the ibuprofen solution is used for pouring seedling seedlings in the seedling stage of the plants, the ibuprofen solution is poured after the ibuprofen solution is soaked for 12-14 hours each time after the ibuprofen solution is poured, the ibuprofen solution is poured once every 5-7 days, and the ibuprofen solution is continuously poured for 3-5 times.
6. The application of ibuprofen in improving salt tolerance of plants according to claim 4, wherein when the ibuprofen solution is used for spraying seedlings of the plants in the seedling stage, the spraying volume of the ibuprofen solution is 0.3-1 mL/plant, and the ibuprofen solution is sprayed once every 4-5 days and is continuously sprayed for 5-6 times.
7. The use of ibuprofen according to claim 1 for increasing salt tolerance in plants, wherein said ibuprofen is capable of increasing seed germination in plants under salt stress.
8. The use of ibuprofen according to claim 1 for improving salt tolerance of plants, wherein the ibuprofen can improve plant height, root length and fresh weight of plants under salt stress.
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