CN112088880B - Application of apigenin in preparation of preparation for promoting plant growth and improving plant salt tolerance - Google Patents
Application of apigenin in preparation of preparation for promoting plant growth and improving plant salt tolerance Download PDFInfo
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- CN112088880B CN112088880B CN202010854231.4A CN202010854231A CN112088880B CN 112088880 B CN112088880 B CN 112088880B CN 202010854231 A CN202010854231 A CN 202010854231A CN 112088880 B CN112088880 B CN 112088880B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses an application of apigenin in preparation of a preparation for promoting plant growth and improving plant salt tolerance. Apigenin as a plant metabolite can play a role in the process of plant resistance to oxidative stress. When the apigenin is applied to plant seeds stressed by salt, the germination rate and the seed germination speed of the seeds under the salt stress can be obviously improved; the plant height, root length, fresh weight or survival rate of the plant seedlings under salt stress can be obviously improved, and the salt stress resistance of the plants is improved; the application of apigenin under normal conditions can also promote plant growth, increase bud length, root length and fresh weight. Therefore, the apigenin plays an important role in protecting and strengthening seedlings and improving salt stress. The application method of the invention is simple to use and has obvious effect.
Description
Technical Field
The invention belongs to the field of biology, and particularly relates to application of apigenin in preparation of a preparation for promoting plant growth and improving plant salt tolerance.
Background
The preparation method of the apigenin comprises the following steps of,
belongs to flavone, and mainly exists in Thymelaeaceae, Verbenaceae and Selaginellaceae plants, such as flos Genkwa and herba Selaginellae with high content. Has carcinogenic activity of carcinogenic substance inhibiting effect, and can be used as antiviral drug for treating HIV and other virus infection; can be used for treating various inflammations; can resist oxidation; has effects of tranquilizing mind; and (4) the efficacy of reducing blood pressure. As a metabolite endogenous to the plant, against the plantIn the process of resisting salt stress, the content of apigenin is greatly changed, which indicates that the apigenin possibly participates in the process of resisting salt stress of plants.
Soil salinization seriously affects the growth and quality of crops, and becomes one of the main environmental factors limiting the yield of the current crops. High salt ultimately leads to a reduction in economic crop yield by affecting seed germination, seedling growth and vigor, flowering and fruiting of plants, etc. (Sairam & Tyagi, 2004). Therefore, the efficient utilization of saline soil and the improvement of the salt resistance of crops have become global and widely concerned problems.
Disclosure of Invention
In view of the situations of the prior art, the invention aims to provide the application of apigenin in preparing a preparation for promoting plant growth and improving plant salt tolerance.
In order to realize the purpose of the invention, the invention is realized by adopting the following technical scheme:
the invention provides application of apigenin in preparation of a preparation for promoting plant growth.
The invention also provides application of the apigenin in preparation of a preparation for improving the salt tolerance of plants.
Further, the specific application method is as follows: applying an aqueous solution of the formulation to plant seeds or seedlings.
Further, the application comprises the following specific steps:
sterilizing the surface of the plant with ethanol, washing with sterile water, and soaking the plant seed in the water solution of the preparation; or soaking the roots of young plants in the aqueous solution of the preparation.
Furthermore, the concentration of the apigenin in the aqueous solution of the preparation is 0.4-0.7 mu mol/L.
Further, the plant seeds are soaked for 12-16 h; the soaking time of the roots of the plant seedlings is 4 to 7 days.
Further, the plant seeds or seedlings are seeds or seedlings of wheat, millet and tobacco.
Furthermore, the apigenin can improve the germination rate of seeds, the germination potential of the seeds, the bud length of seedlings, the root length of seedlings, the fresh weight of the seedlings and the survival rate of the seedlings.
Compared with the prior art, the invention has the advantages and positive effects that: the invention provides application of apigenin in promoting plant growth and improving plant salt tolerance, and the apigenin serving as a plant metabolite can play a role in plants. The growth can be promoted by applying apigenin aqueous solution under normal conditions; the application of the fertilizer under the condition of salt stress can improve the germination rate and the germination vigor of seeds and reduce salt damage; can also improve the height, the root length, the fresh weight and the survival rate of the plants, and can achieve the purposes of protecting and strengthening the seedlings and improving the salt stress resistance in production. The invention provides the application of apigenin in promoting plant growth and improving plant salt tolerance, and the application is simple and has obvious effect.
Drawings
Fig. 1 is a growth promoting effect of apigenin on wheat, millet and tobacco seeds, wherein A: growth promoting effect of apigenin on wheat seeds, B: growth promoting effect of apigenin on millet seeds, C: the growth promotion effect of the apigenin on tobacco seeds is realized;
FIG. 2 shows the growth promoting effect of apigenin on wheat seedlings;
FIG. 3 shows the growth promoting effect of apigenin on millet seedlings;
FIG. 4 shows the growth promoting effect of apigenin on tobacco seedlings;
FIG. 5 shows the salt tolerance promoting effect of apigenin on wheat seeds, millet seeds and tobacco seeds; wherein, A: promoting effect of apigenin on salt tolerance of wheat seeds, wherein B: promoting effect of apigenin on salt tolerance of millet seeds, wherein the ratio of apigenin to salt is as follows: promoting the salt tolerance of tobacco seeds by using apigenin;
FIG. 6 shows the effect of apigenin on the promotion of salt tolerance of wheat seedlings;
FIG. 7 shows the effect of apigenin on the promotion of salt tolerance of millet seedlings;
FIG. 8 shows the effect of apigenin on the promotion of salt tolerance of tobacco seedlings.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to the accompanying drawings and the detailed description.
Example 1 apigenin can promote plant growth: seed germination and seedling growth
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. And (3) soaking the seeds of the control group and the experimental group in corresponding treatment solutions for 12 hours respectively for pretreatment. Three replicates of each treatment, each 50 seeds in parallel.
The soaked seeds were spread on a 9 cm glass petri dish previously filled with sterilized filter paper and cultured at 25 ℃.5 ml of the corresponding treatment solution was added to each dish. And counting germination data every day, and counting bud length, root length and fresh weight. The germination rate of the seeds is the germination rate of wheat on the 2 nd day, the germination rate of the millet on the 3 rd day and the germination rate of the tobacco on the 5 th day; the germination vigor of wheat is the germination vigor of 4 days, the germination vigor of millet is the germination vigor of 5 days, and the germination vigor of tobacco is the germination vigor of 7 days; seedling height, root length and fresh weight, wheat is the plant height, root length and fresh weight at the 4 th day after treatment, millet is the plant height, root length and fresh weight at the 5 th day after treatment, and tobacco is the plant height, root length and fresh weight at the 7 th day after treatment.
TABLE 1 growth promoting effect of apigenin on wheat, millet and tobacco seeds
Note:＊＊＊P＜0.001，＊＊P＜0.01，＊P＜0.05。
in the table, CK is distilled water and CK + 0.5. mu. MAP is an aqueous solution containing 0.5. mu.M apigenin.
Germination rate is 100% of the number of germinated seeds/total number of seeds.
Germination vigor is 100% of the number of normally germinated seeds/total number of test seeds within the number of days of germination.
As can be seen from table 1 and A, B of fig. 1, exogenous application of apigenin significantly promoted plant seed germination and post-germination growth compared to controls.
2. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. After wheat sprouts for 2 days, millet sprouts for 3 days and tobacco sprouts for 5 days, selecting seedlings with consistent growth vigor, and carrying out corresponding solution treatment. And (5) observing the phenotype. And counting the bud length, the root length and the fresh weight. As can be seen from fig. 2, 3 and 4, exogenous application of apigenin under normal conditions significantly promoted the growth of plants compared to controls.
Example 2 apigenin can improve salt tolerance of plant seeds
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. The seeds of the control group and the experimental group are respectively soaked in the treatment solution for 12 hours for pretreatment. Three replicates of each treatment, 50 seeds per replicate.
The soaked seeds were spread on a 9 cm glass petri dish previously filled with sterilized filter paper and cultured at 25 ℃.5 ml of the corresponding treatment solution was added to each dish. Germination data were counted daily. The germination rate of the seeds is the germination rate of wheat on the 2 nd day, the germination rate of the millet on the 3 rd day and the germination rate of the tobacco on the 5 th day; the germination vigor of wheat is the germination vigor of 4 days, the germination vigor of millet is the germination vigor of 5 days, and the germination vigor of tobacco is the germination vigor of 7 days; seedling height, root length and fresh weight, wheat is the plant height, root length and fresh weight at the 4 th day after treatment, millet is the plant height, root length and fresh weight at the 5 th day after treatment, and tobacco is the plant height, root length and fresh weight at the 7 th day after treatment.
TABLE 2 promoting effect of apigenin on salt tolerance of wheat, millet and tobacco seeds
Note:＊＊＊P＜0.001，＊＊P＜0.01，＊P＜0.05
the concentration of NaCl solution in the table was 150mmol/L, and NaCl + 0.5. mu.M AP was an aqueous solution containing 150mmol/L NaCl solution and 0.5. mu. mol/L apigenin. Germination rate is 100% of the number of germinated seeds/total number of seeds. Germination vigor is 100% of the number of normally germinated seeds/total number of test seeds within the number of days of germination.
It is clear from table 2 and A, B, C of fig. 5 that exogenous application of apigenin significantly promoted germination and growth of wheat, millet and tobacco seeds under salt stress, as compared to the control.
Example 3 apigenin can improve salt tolerance of young plants
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. After wheat sprouts for 2 days, millet sprouts for 3 days and tobacco sprouts for 5 days, selecting seedlings with consistent growth vigor, and carrying out corresponding solution treatment. And (5) observing the phenotype. And counting the bud length, the root length, the fresh weight and the survival rate.
As can be seen from fig. 6, 7 and 8, exogenous application of apigenin under salt stress conditions significantly promoted the growth of plants, compared to the control.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (5)
1. The application of apigenin in preparing a preparation for promoting plant growth is characterized in that: the specific application mode is as follows: applying an aqueous solution of the formulation to plant seeds or seedlings; the plant seeds or seedlings are seeds or seedlings of tobacco; the concentration of the apigenin in the aqueous solution of the preparation is 0.4-0.7 mu mol/L.
2. The application of apigenin in preparing a preparation for improving the salt tolerance of plants is characterized in that: the specific application mode is as follows: applying an aqueous solution of the formulation to plant seeds or seedlings; the plant seeds or seedlings are seeds or seedlings of tobacco; the concentration of the apigenin in the aqueous solution of the preparation is 0.4-0.7 mu mol/L.
3. Use according to claim 1 or 2, characterized in that: the application comprises the following specific steps: sterilizing the surface of the plant with ethanol, washing with sterile water, and soaking the plant seed in the water solution of the preparation; or soaking the roots of young plants in the aqueous solution of the preparation.
4. Use according to claim 3, characterized in that: the soaking time of the plant seeds is 12-16 h; the soaking time of the roots of the plant seedlings is 4 to 7 days.
5. Use according to claim 1 or 2, characterized in that: the apigenin can improve seed germination rate, improve seed germination vigor, increase seedling bud length, increase seedling root length, increase seedling fresh weight and increase seedling survival rate.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211857A (en) * | 2011-09-08 | 2016-12-14 | 诺维信生物农业公司 | Method for treating seeds and compositions |
| CN106386871A (en) * | 2016-08-31 | 2017-02-15 | 山东胜伟园林科技有限公司 | Rooting agent containing celery extract and preparation method thereof |
| CN108174872A (en) * | 2018-01-11 | 2018-06-19 | 和县农丰蔬菜种子专业合作社 | A kind of vegetable seeds sterilization and activation inorganic agent and its preparation and application |
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- 2020-08-21 CN CN202010854231.4A patent/CN112088880B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211857A (en) * | 2011-09-08 | 2016-12-14 | 诺维信生物农业公司 | Method for treating seeds and compositions |
| CN106386871A (en) * | 2016-08-31 | 2017-02-15 | 山东胜伟园林科技有限公司 | Rooting agent containing celery extract and preparation method thereof |
| CN108174872A (en) * | 2018-01-11 | 2018-06-19 | 和县农丰蔬菜种子专业合作社 | A kind of vegetable seeds sterilization and activation inorganic agent and its preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| Ahmad Mohammad M. Mkeawy etl.Apigenin pretreatment enhances growth and salinity tolerance of rice Seedlings.《Plant physiology and Biochemistry》.2018, * |
| Apigenin pretreatment enhances growth and salinity tolerance of rice Seedlings;Ahmad Mohammad M. Mkeawy etl;《Plant physiology and Biochemistry》;20180630;摘要、2材料和方法、3结果部分、图表1A-C * |
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