CN109220280A - A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation - Google Patents
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2105/00—Erosion prevention
Abstract
The present invention relates to seedling fostering field, in particular to a kind of engrafting method for promoting diversiform-leaved poplar vegetative propagation.A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps: take the edible tender branch of diversiform-leaved poplar, bud tablets are made；Bud interface is prepared on the nursery stock of Xinjiang poplar, the bud interface is sprayed with mixed liquor, in terms of the volume of mixed liquor, gibberellin 100-500mg/L, indole-3-acetic acid 50-200mg/L, methyl α-naphthyl acetate 50-200mg/L, potassium dihydrogen phosphate 400-800mg/L and calcium superphosphate 500-1000mg/L are contained in the mixed liquor；Bud tablets are inserted into bud interface, bundle bud socket part position, complete grafting.The survival rate of this method grafting can reach 75% or more, and required diversiform-leaved poplar material is greatly decreased, and whole process operation is convenient, time saving and energy saving, be conducive to promote and apply on a large scale.
Description
Technical field
The present invention relates to seedling fostering fields, in particular to a kind of engrafting method for promoting diversiform-leaved poplar vegetative propagation.
Background technique
Diversiform-leaved poplar is distributed mainly on northwest China desert and other arid sand dune areas, is the distinctive precious forest money in Desert Area
" desert patron saint " is described as by people in source, has the critically important ecological value.In addition, it also has critically important medicine effect
And economic value.
Diversiform-leaved poplar itself mainly uses seminal propagation, and still, artificial to carry out the differentiation of seminal propagation nursery stock seriously, growth is irregular not
Together, it is difficult to become a useful person, and be easy to obtain rust during the growth process, limit the development of diversiform-leaved poplar artificial forest.In addition, diversiform-leaved poplar is asexual numerous
It grows and promotees tiller, cuttage and grafting etc. including being completely cured, but the complicated difficulty of hardwood cutting method, survival rate are low, and maintain diversiform-leaved poplar from long-term
Woods considers that being completely cured promotees tiller and not can solve substantive issue.Original with power of regeneration after graft technology mainly utilizes plant injured
Reason, has many advantages, such as easy to operate, high survival rate.
Research in terms of existing diversiform-leaved poplar graft technology is concentrated mainly on small U.S. drought, Xinjiang poplar, Gansu Poplar, willow, Mao Bai
Poplar is to graft diversiform-leaved poplar on stock, or using diversiform-leaved poplar as stock grafting Xinjiang poplar, graft survival rate all achieves success, regrettably transfers
Seedlings picking fails to show the good characteristic of diversiform-leaved poplar, is still difficult to solve the problems, such as the vegetative propagation of diversiform-leaved poplar.
In order to solve this contradiction, application No. is 201210461262.9 to disclose a kind of transferring for promotion diversiform-leaved poplar vegetative propagation
Method is connect, method includes the following steps: (1) irrigation and the prevention and control of plant diseases, pest control before grafting；(2) the selection of stock: selection trunk is logical straight,
It is annual, growth is vigorous and full scion Xinjiang poplar is as stock；(3) the selection of diversiform-leaved poplar scion: between twenty and fifty diversiform-leaved poplar tree is selected to make
For kind of an item；It takes kind of the full position in item centre comprising 4~6 buds and length is the branch of 8~12cm as diversiform-leaved poplar scion；(4) in anvil
It is divided into " ten " font edge of a knife with grafting knife at 1~1.2m height of the wooden Xinjiang poplar trunk, and pries open and splits mouth；(5) by diversiform-leaved poplar scion
Trim diamondwise, the diversiform-leaved poplar scion after being trimmed in lower end；The Ching Ming Festival before and after by after trimming diversiform-leaved poplar scion insertion split mouth
Place, tightens, and completes grafting；(7) pour water, apply fertilizer in time after the completion of grafting；Xinjiang poplar occurs sprouting when bud that the bud of sprouting is complete
Portion is erased, until pesticide when diversiform-leaved poplar seedling grows 3~4 true leaves.I.e. the invention is to be grafted onto the branch of diversiform-leaved poplar newly
Vegetative propagation, but a large amount of diversiform-leaved poplar branch of this breeding demand are carried out on boundary poplar, and entire step is complex, grafting survival
Rate also needs to be further improved.
In view of this, the present invention is specifically proposed.
Summary of the invention
The purpose of the present invention is to provide it is a kind of promote diversiform-leaved poplar vegetative propagation engrafting method, this method using Xinjiang poplar as
Stock carries out bud after the two processing and connects, raw material is easy to get, and saving of work and time, graft survival rate reaches using the bud tablets of diversiform-leaved poplar as scion
75% or more, be conducive to be widely applied on a large scale.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps:
Bud tablets preparation: taking the edible tender branch of diversiform-leaved poplar, and bud tablets are made；
Bud tablets processing: the bud tablets are impregnated into 10min-120min before bud connects in plant nutrition liquid, in the plant
Nutrient solution includes stock extracting solution；
Stock processing: selecting eugonic Xinjiang poplar carpentery workshop is stock, and bud interface is prepared on the nursery stock of Xinjiang poplar, is used
Mixed liquor sprays the bud interface, in terms of the volume of the mixed liquor, in the mixed liquor containing gibberellin 100-500mg/L,
Indole-3-acetic acid 50-200mg/L, methyl α-naphthyl acetate 50-200mg/L, potassium dihydrogen phosphate 400-800mg/L and calcium superphosphate 500-
1000mg/L；
It grafts bud tablets: the bud tablets being inserted into the bud interface, bundle bud socket part position, obtain grafting nursery stock.
Soil treatment: soil conditioner is added in the root of the grafting nursery stock, completes grafting.
The present inventor is grafted, but operating to reduce the triviality of diversiform-leaved poplar vegetative propagation in such a way that bud connects
It finds in the process, directly by the bud interface of bud tablets insertion Xinjiang poplar, the survival rate finally grafted is very low, probably in 40%-
50%；Inventor has found through test of many times, after bud interface is handled with mixed liquor of the invention, then carries out bud and connects, grafting survives
Rate can reach 77% or more, and required diversiform-leaved poplar material is greatly decreased, and whole process operation is convenient, time saving and energy saving, be conducive to
It is a wide range of to promote and apply.
Secondly, inventors be surprised to learn that, bud tablets are impregnated in plant nutrition liquid, the fecundity of bud tablets becomes strong, and then makes
It obtains graft survival rate further to be promoted, the amplitude of promotion is 5% or more.
Finally, stock extracting solution is added in plant nutrition liquid, so that bud tablets are easy later on being grafted onto stock and anvil
Wood fusion, improves the survival rate of grafting；Percentage by volume of the stock extracting solution in the culture solution is 5%-30%.In addition,
It is found in test, the juice for adding excessive content is easy contaminated, therefore, adds stock extracting solution appropriate and had both reached good
Fusion is grafted, and it is infected to be unlikely to bud tablets.
Preferably, the soil conditioner, in parts by weight, material component includes:
100-150 parts of diatomite, 80-120 parts of flyash, 70-90 parts of bentonite, 50-70 parts of kaolin, turf 80-120
Part, 120-160 parts of plant straw powder, -20 parts of carrier 12 containing algae, 40-60 parts of organic fertilizer, 40-70 parts of humus, zeolite powder
20-35 parts, 20-30 parts of glass microballoon, 18-25 parts of Cab-O-sil, 3-5 parts of hydrophobic resin, 20-30 parts of microbial inoculum；
10-20 parts of POLYPROPYLENE GLYCOL, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of waste residue of Chinese herbs powder, useless tea grounds
20-40 parts, 8-24 parts of fulvic acid concentrate, 40-60 parts of vinasse, 30-60 parts of leaf mould, 40-60 parts of sawdust, rice chaff ash 20-30
Part, loose 10-30 parts of phosphorus, 50-70 parts of coco bran and 30-40 parts of slow-release fertilizer.
In this soil conditioner provided by the invention, the various components with specific compatibility relationship are selected, specifically may be used
To be divided into improvement component and component of fixing the sand.It wherein, is main with POLYPROPYLENE GLYCOL, hydrophobic resin and bentonite in component of fixing the sand
Component, to realize the function fixed to loose soil (sand) matter, the molding layer that fixes the sand of formation, more it is essential that in this application,
Also contain changing with compositions such as kaolin, flyash, diatomite, turf, organic fertilizer, humus, waste residue of Chinese herbs powder and useless tea grounds
Good component.These components are especially advantageous for the environment of stock growth according to that can be formed after set compatibility relationship proportion；And the ring
Border is also especially advantageous for rapid microbial growth metabolism (fulvic acid concentrate, humus play main effect)；Fulvic acid concentrate
With humus collective effect, it can be used for improving stock root microenvironment；Promote the conversion of the matter and energy of stock root, thus
Stock is set to show the situation of health；Simultaneously, moreover it is possible to inhibit the growth of harmful fungoid, and supply and supported needed for grafting seedling growth
Point, promote grafting seedling stage root system development growth；Action temperature and, it is biodegradable.It should be strongly noted that being opened in the application
Invasive low compatibility is useless with carrier containing algae, glass microballoon, Cab-O-sil, microbial inoculum, fulvic acid concentrate, useless tea grounds, Chinese medicine
The improvement component of the compositions such as ground-slag, rice chaff ash, after several component mix and match, it has unexpectedly been found that, the combination is for stock institute
There is the low raising of highly significant in the raising of the water-retaining property and gas permeability of soil, improve the survival rate of grafting nursery stock.
In addition, the plant straw powder includes following component by weight percentage:
Milled powders of cornstalk 8-20%, maize cob meal 6-20%, potato straw powder 9-25%, sea-buckthorn straw powder 10-30%, camel
Pierce straw powder 10-15%, rice straw powder 10-15%, flax straw powder 8-15%, wheat stalk powder 5-15%, broomcorn straw
Powder 5-20% and pea straw powder 5-20%.
The microbial inoculum is by one of actinomyces, nitrogen-fixing bacteria, bacillus subtilis, photosynthetic bacteria, saccharomycete
Or the bacterium solution after a variety of cultivation and fermentations.
After complex microorganism bacterium solution and the effect of organic biofertilizer and humic acid, a series of metabolites can be generated, are led to
It crosses the competition to space and nutrition and generates a variety of antibacterial pest-resistant substances, play the pest-resistant purpose of diseases prevention.In addition, can also stimulate
Nursery stock growth and development is grafted, crop anti-adversity is improved, promotes root growth and development.
Vermiculite power has good cationic exchangeability, adsorptivity, water-retaining property, improves root system surface microenvironment, water storage is saturating
Gas；The pH value of root system ambient enviroment can be buffered, keeps nutrient slowly-released, mineral element is provided, promote seedling stage plant growth.
Fixative plays filming function, other each components is enabled smoothly to be coated on root system surface.According to above-mentioned
Weight ratio mixes and manufactured soil conditioner, while each component plays respective effect, can also generate between different components
Synergistic effect can realized so that coating agent generates the Related Components such as antibacterial, insect prevention, organic-inorganic nutrient, positive growth factor
Whiles promoting sproutings of stock root system, preventing disease and pest, increase production and improve productivity etc., the survival rate for grafting nursery stock is improved.Even more important
It is that each component of the coating agent is pure natural component, it is biodegradable, it is non-hazardous to people and animals and environment, meet agricultural production
Non-harmful requirement.
The carrier containing algae, which is negative, is loaded with total ball algae, false ball algae, column silk algae, Scytonema arcangelii, sheath silk algae, micro- sheath algae, fish raw meat altogether
Two or more macromolecule polymeric material in algae, slimeball algae and nostoc.
There is stronger breeding energy in view of total ball algae, false ball algae, column silk algae, Scytonema arcangelii, sheath silk algae and micro- sheath algae etc. altogether
Power and resistance ability, meanwhile, mostly in lichens parasymbiosis, and the combination of several algae can also embody the effect of synergy
Fruit.It is therefore preferable that using above-mentioned several algae as breeding kind.In addition, carrier containing algae preferably has preferable water holding capacity,
And readily flowed macromolecule polymeric material, it is intended to improve the survival ability and propagation performance of algae.
In order to increase the effect of mixed liquor, increase the survival rate of grafting, it is preferable that contain gibberellin in the mixed liquor
200-400mg/L, indole-3-acetic acid 100-150mg/L, methyl α-naphthyl acetate 100-120mg/L, potassium dihydrogen phosphate 500-600mg/L and
Calcium superphosphate 700-800mg/L.
Preferably, the soil conditioner is made by the following steps:
1), kaolin and flyash are uniformly mixed, and are added that water stirring is agglomerating, and dough is placed under the conditions of 30-35 DEG C
Heating 1-1.5 hours, obtains the first mixture；
2) fulvic acid concentrate is added after, mixing diatomite and turf, adds water and continues to mix, obtain the second mixing
Material；
3) straw decomposing inoculant decomposed rear and organic fertilizer, humus, waste residue of Chinese herbs powder and useless tea, is added in plant straw powder
Slag mixing, and mix after coco bran mixes and mix thoroughly with vinasse, leaf mould, sawdust, rice chaff ash and loose phosphorus after heap fermentation, and in 20-
30 DEG C stand 2-3 days, obtain third mixture；
4) hydrophobic resin is added after, mixing zeolite powder, glass microballoon and Cab-O-sil to stir evenly；Obtain the 4th mixing
Material；
5) urea, monoammonium phosphate and slow-release fertilizer are added after, mixing second mixture, the third mixture, and
Dispersion stirring is carried out, then first mixture and the 4th mixture are added thereto, is stirring evenly and then adding into and is carried containing algae
Body and microbial inoculum continue under paving after mixing to 25-30 DEG C of environment 2-5 hours, obtain pre- using powder；
6) quality is its 2-4 times water and quality is its 1%-4% inorganic salts, are added in bentonite, it is agitated or
Extrusion modification, then be classified and purified, obtain slurries of the granularity 90% less than 10 μm；By the slurries and have been dissolved in water
POLYPROPYLENE GLYCOL aqueous solution, water carry out mixture, and be individually encapsulated after mixing evenly with 20-30 revs/min of revolving speed, obtain pre- use
Liquid material.
Preferably, ultrasonication is also carried out when the bud tablets are immersed in plant nutrition liquid, wherein the power of ultrasonic wave
For 100-200W, each ultrasound 5-8s, it is adjacent it is ultrasonic twice between be divided into 20-30s.
By ultrasonication, the time of bud tablets immersion can be shortened, further promote graft survival rate, also, graft
Successful plant is not easy sick.
Preferably, the bud tablets band xylem, 1-3 centimetres of the lower end ion leaf of the bud interface.
Bud tablets band xylem, not only makes the acquisition of bud tablets more easy, saves the tedious steps of stripping bud, and prevent from shelling
Bud damages bud tablets bring.
If bud interface ion leaf is too close, when operation, is easy to encounter cotyledon, and cotyledon falls off, and increases operation difficulty；And if ion
Leaf is too far, is unfavorable for nutrition supply.Further, 1-3 centimetres of the lower end ion leaf of the bud interface.As bud interface lower end from
Cotyledon can be 1 centimetre, 1.5 centimetres, 2 centimetres, 2.5 centimetres, 3 centimetres etc..
It is preferably, described to prepare bud interface specifically:
Xinjiang poplar nursery stock stem output on one side a length of 2-3 centimetres, width be 2/5-3/5 stem week bud interface；
Water cannot be poured in 10 days after grafting and pour bud socket part position.
Preferably, the binding are as follows: bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt.
Bud tablets are not slided when binding, bundle elastic appropriateness, and whole process is convenient and efficient, reduces the amount of labour and labour is strong
Degree.
Preferably, the stock extracting solution has the following steps to be made:
The water of the rhizome of Xinjiang poplar, 5~10 times of strange willow rhizome quality is subjected to 1~2h of hyperbaric heating, filter is obtained by filtration
Liquid and filter residue；
Filter residue and filtrate are placed in closed container, zymophyte is accessed in a reservoir and carries out 4~6h of fermentation；
Stock extracting solution is obtained by filtration after fermentation.
It is found through overtesting, the stock extracting solution obtained after filtrate and filter residue are fermented can further increase grafting
The survival rate of nursery stock.
In addition, it is necessary to explanation, the management after present invention grafting is carried out according to the breeding method of conventional seedling, according to
The demand of plant growth reinforces water and fertilizer management.
Compared with prior art, the invention has the benefit that
(1) after the present invention carries out mixed liquor processing to bud interface, then carry out bud and connect, the survival rate of grafting can reach 75% with
On.
(2) present invention also impregnates bud tablets using plant nutrition liquid, has been surprisingly found that, the fecundity of bud tablets becomes strong,
So that graft survival rate is further promoted, the amplitude of promotion is 5% or more.
(3) ultrasonication is carried out while the present invention also impregnates bud tablets, not only shortens the time that bud tablets are impregnated, into one
Step promotes graft survival rate, also, graft successful plant be not easy it is sick.
(4) present invention also defines the processing methods of bud tablets, bud interface and binding, and whole process operation is convenient, reduce
The amount of labour and labor intensity.
(5) the invention to stock soil carry out improved treatment, improve the growth activity of stock, promote grafting
Survival rate.
(6) present invention adds stock extracting solution in plant nutrition liquid, so that bud tablets are easy later on being grafted onto stock
It is merged with stock, improves the survival rate of grafting.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Embodiment 1
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps:
The edible tender branch of diversiform-leaved poplar is taken, bud tablets, bud tablets band xylem is made；
The bud tablets are impregnated in plant nutrition liquid before bud connects, include stock extracting solution, the anvil in plant nutrition liquid
The wooden extracting solution in mass 5%；
A length of 2 centimetres, bud interface of the width for 2/5 stem week, the lower end of bud interface are outputed on one side in Xinjiang poplar nursery stock stem
1 centimetre of ion leaf, bud interface is sprayed with mixed liquor, in terms of the volume of mixed liquor, gibberellin 100mg/L, Yin are contained in mixed liquor
Diindyl -3- acetic acid 50mg/L, methyl α-naphthyl acetate 200mg/L, potassium dihydrogen phosphate 400mg/L and calcium superphosphate 1000mg/L；
Bud tablets are inserted into bud interface, and bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt, bundle elastic appropriateness, obtain
To grafting nursery stock.
Soil conditioner is added in the root of the grafting nursery stock, completes grafting.
Management after grafting is carried out according to the breeding method of conventional seedling, is reinforced one according to the demand of plant growth and is lauched
Fertilizer management.
Five groups are set altogether, and every group grafting 100, the time that bud tablets unlike between group are impregnated in plant nutrition liquid divides
It Wei not 10min, 30min, 60min, 90min, 120min.
The bud take of this five groups is counted, it is specific as shown in table 1.
Bud take between the different groups of table 1
| Soaking time | Bud take |
| 10min | 85% |
| 30min | 88% |
| 60min | 90% |
| 90min | 90% |
| 120min | 86% |
As it can be seen that bud tablets soaking time in plant nutrition liquid is 30-90min than convenient.
Embodiment 2
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps:
The edible tender branch of diversiform-leaved poplar is taken, bud tablets are made；
The bud tablets are impregnated into 60min before bud connects in plant nutrition liquid, include stock extracting solution in plant nutrition liquid,
The stock extracting solution in mass 15%, also carries out ultrasonication, wherein ultrasonic wave when bud tablets are immersed in plant nutrition liquid
Power be 200W, each ultrasound 5s, it is adjacent it is ultrasonic twice between be divided into 20s；
A length of 3 centimetres, bud interface of the width for 2/5 stem week, the lower end of bud interface are outputed on one side in Xinjiang poplar nursery stock stem
3 centimetres of ion leaf, bud interface is sprayed with mixed liquor, in terms of the volume of mixed liquor, gibberellin 500mg/L, Yin are contained in mixed liquor
Diindyl -3- acetic acid 200mg/L, methyl α-naphthyl acetate 50mg/L, potassium dihydrogen phosphate 800mg/L and calcium superphosphate 500mg/L；
Bud tablets are inserted into bud interface, and bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt, bundle elastic appropriateness, obtain
To grafting nursery stock.
Soil conditioner is added in the root of the grafting nursery stock, completes grafting.
Management after grafting is carried out according to the breeding method of conventional seedling, is reinforced one according to the demand of plant growth and is lauched
Fertilizer management.
Grafting 100, through counting, this method bud take is 93%.
Embodiment 3
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps:
The edible tender branch of diversiform-leaved poplar is taken, bud tablets are made；
The bud tablets are impregnated into 60min before bud connects in plant nutrition liquid, include stock extracting solution in plant nutrition liquid,
The stock extracting solution in mass 15%, also carries out ultrasonication, wherein ultrasonic wave when bud tablets are immersed in plant nutrition liquid
Power be 200W, each ultrasound 5s, it is adjacent it is ultrasonic twice between be divided into 20s；
A length of 3 centimetres, bud interface of the width for 2/5 stem week, the lower end of bud interface are outputed on one side in Xinjiang poplar nursery stock stem
3 centimetres of ion leaf, bud interface is sprayed with mixed liquor, in terms of the volume of mixed liquor, gibberellin 500mg/L, Yin are contained in mixed liquor
Diindyl -3- acetic acid 200mg/L, methyl α-naphthyl acetate 50mg/L, potassium dihydrogen phosphate 800mg/L and calcium superphosphate 500mg/L；
Bud tablets are inserted into bud interface, and bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt, bundle elastic appropriateness, obtain
To grafting nursery stock.
Soil conditioner is added in the root of the grafting nursery stock, completes grafting.
Wherein, soil conditioner is made for following raw materials: 120 parts of diatomite, 100 parts of flyash, 80 parts of bentonite, kaolinite
Native 60 parts, 100 parts of turf, 140 parts of plant straw powder, 16 parts of carrier containing algae, 50 parts of organic fertilizer, 13 parts of coating grass-seed, humic
55 parts of matter, 30 parts of zeolite powder, 25 parts of glass microballoon, 20 parts of Cab-O-sil, 4 parts of hydrophobic resin, 25 parts of microbial inoculum；
15 parts of POLYPROPYLENE GLYCOL, 12 parts of urea, 12 parts of monoammonium phosphate, 35 parts of waste residue of Chinese herbs powder, useless 30 parts of tea grounds, fulvic acid are dense
16 parts of contracting liquid, 50 parts of vinasse, 45 parts of leaf mould, 50 parts of sawdust, 250 parts of rice chaff ash, loose 20 parts of phosphorus, 60 parts of coco bran and slow-release fertilizer 35
Part.
Wherein, plant straw powder includes following component by weight percentage:
Milled powders of cornstalk 8%, maize cob meal 14%, potato straw powder 25%, sea-buckthorn straw powder 10%, camel thorn straw powder
10%, rice straw powder 10%, flax straw powder 8%, wheat stalk powder 5%, broomcorn straw powder 5% and pea straw powder 5%；
Microbial inoculum is by actinomyces, nitrogen-fixing bacteria, bacillus subtilis, photosynthetic bacteria, saccharomycete, huge gemma bar
Bacterium solution after bacterium, sulfate reducing bacteria cultivation and fermentation；
Carrier containing algae, which is negative, to be loaded with total ball algae, false ball algae altogether, column silk algae, Scytonema arcangelii, sheath silk algae, micro- sheath algae, anabena, glues
The macromolecule polymeric material of ball algae and nostoc.
Preparation method
S31, kaolin and flyash are uniformly mixed, and it is agglomerating to be added water stirring, under the conditions of dough is placed in 32 DEG C plus
Heat 1.5 hours, obtains the first mixture；
S32, fulvic acid concentrate is added after mixing diatomite and turf, adding water to continue to mix (can extremely flow
It is thick), obtain the second mixture；
S33, plant straw powder is added to the decomposed rear and organic fertilizer of straw decomposing inoculant, humus, waste residue of Chinese herbs powder and is given up
Tea grounds mixing, and mix after coco bran mixes and mix thoroughly with vinasse, leaf mould, sawdust, rice chaff ash and loose phosphorus after heap fermentation, and in 25
DEG C stand 3 days, obtain third mixture；
S34, will zeolite powder, glass microballoon and Cab-O-sil mix after be added hydrophobic resin stir evenly；It is mixed to obtain the 4th
Close material；
S35, urea, monoammonium phosphate and slow-release fertilizer is added after mixing second mixture, the third mixture, and
Dispersion stirring is carried out, then first mixture and the 4th mixture are added thereto, is stirring evenly and then adding into and is carried containing algae
Body and microbial inoculum continue to pave to 28 DEG C of environment lower 4 hours after mixing, and obtain pre- powder, i.e. soil conditioner；
Management after grafting is carried out according to the breeding method of conventional seedling, is reinforced one according to the demand of plant growth and is lauched
Fertilizer management.
Grafting 100, through counting, this method bud take is 96%.
Embodiment 4
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps:
The edible tender branch of diversiform-leaved poplar is taken, bud tablets are made, bud tablets are impregnated 60min by bud tablets band xylem in plant nutrition liquid,
It does not include stock extracting solution in the nutrient solution；
A length of 3 centimetres, bud interface of the width for 2/5 stem week, the lower end of bud interface are outputed on one side in Xinjiang poplar nursery stock stem
2 centimetres of ion leaf, bud interface is sprayed with mixed liquor, in terms of the volume of mixed liquor, gibberellin 400mg/L, Yin are contained in mixed liquor
Diindyl -3- acetic acid 150mg/L, methyl α-naphthyl acetate 120mg/L, potassium dihydrogen phosphate 600mg/L and calcium superphosphate 700mg/L；
Bud tablets are inserted into bud interface after pulling out, and bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt, bundle elastic suitable
Degree obtains grafting nursery stock.
Soil conditioner is added in the root of the grafting nursery stock, completes grafting.
Management after grafting is carried out according to the breeding method of conventional seedling, is reinforced one according to the demand of plant growth and is lauched
Fertilizer management.
Grafting 100, through counting, this method bud take is 75%.
Embodiment 5
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps:
The edible tender branch of diversiform-leaved poplar is taken, bud tablets are made, bud tablets are impregnated 25min by bud tablets band xylem in plant nutrition liquid,
Ultrasonication is also carried out when bud tablets are immersed in plant nutrition liquid, wherein the power of ultrasonic wave is 150W, each ultrasound 7s,
25s is divided between adjacent ultrasound twice；
A length of 3 centimetres, bud interface of the width for 2/5 stem week, the lower end of bud interface are outputed on one side in Xinjiang poplar nursery stock stem
2 centimetres of ion leaf, bud interface is sprayed with mixed liquor, in terms of the volume of mixed liquor, gibberellin 200mg/L, Yin are contained in mixed liquor
Diindyl -3- acetic acid 100mg/L, methyl α-naphthyl acetate 100mg/L, potassium dihydrogen phosphate 500mg/L and calcium superphosphate 800mg/L；
Bud tablets are inserted into bud interface after pulling out, and bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt, bundle elastic suitable
Degree obtains grafting nursery stock.
Soil conditioner is added in the root of the grafting nursery stock, completes grafting.
Management after grafting is carried out according to the breeding method of conventional seedling, is reinforced one according to the demand of plant growth and is lauched
Fertilizer management.
Grafting 100, through counting, this method bud take is 97%.
Comparative example 1
Stock: Xinjiang poplar nursery stock；
Scion: the edible tender branch of diversiform-leaved poplar；
It is divided into the cross edge of a knife with grafting knife on stock, horizontally long 8cm, long 10cm is erected, and epidermis is pushed aside, pries open and split mouth,
It splits and smears protective agent (" shield tree general " 100 times of liquid that mass concentration is 1%) at mouth；
Diamondwise is trimmed in the lower end of the edible tender branch of diversiform-leaved poplar, and insertion is split at mouth, tightened, and completes grafting.
Management after grafting is carried out according to the breeding method of conventional seedling, is reinforced one according to the demand of plant growth and is lauched
Fertilizer management.
Grafting 100, through counting, this method bud take is 65%.
Embodiment 6
A kind of engrafting method promoting diversiform-leaved poplar vegetative propagation, comprising the following steps:
The edible tender branch of diversiform-leaved poplar is taken, bud tablets are made, bud tablets are impregnated 25min by bud tablets band xylem in plant nutrition liquid,
It wherein include stock extracting solution in nutrient solution, which is made by the following steps: by the rhizome of Xinjiang poplar, 5 times of monsters
The water of willow rhizome quality carries out hyperbaric heating 2h, and filtrate and filter residue is obtained by filtration；
Filter residue and filtrate are placed in closed container, zymophyte is accessed in a reservoir and carries out fermentation 5h；
Stock extracting solution is obtained by filtration after fermentation.
Ultrasonication is also carried out when bud tablets are immersed in plant nutrition liquid, wherein the power of ultrasonic wave is 150W, every time
Ultrasonic 7s, it is adjacent twice ultrasound between be divided into 25s；
A length of 3 centimetres, bud interface of the width for 2/5 stem week, the lower end of bud interface are outputed on one side in Xinjiang poplar nursery stock stem
2 centimetres of ion leaf, bud interface is sprayed with mixed liquor, in terms of the volume of mixed liquor, gibberellin 200mg/L, Yin are contained in mixed liquor
Diindyl -3- acetic acid 100mg/L, methyl α-naphthyl acetate 100mg/L, potassium dihydrogen phosphate 500mg/L and calcium superphosphate 800mg/L；
Bud tablets are inserted into bud interface after pulling out, and bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt, bundle elastic suitable
Degree obtains grafting nursery stock.
Soil conditioner is added in the root of the grafting nursery stock, completes grafting.
Management after grafting is carried out according to the breeding method of conventional seedling, is reinforced one according to the demand of plant growth and is lauched
Fertilizer management.
Grafting 100, through counting, this method bud take is 97%.
Furthermore control group is arranged in the present embodiment, and stock extracting solution is using the conventional extracting solution that do not ferment in control group, through grafting
100, statistics this method survival rate is 84%.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention
Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of engrafting method for promoting diversiform-leaved poplar vegetative propagation, which comprises the following steps:
Bud tablets preparation: taking the edible tender branch of diversiform-leaved poplar, and bud tablets are made；
Bud tablets processing: the bud tablets are impregnated into 10min-120min before bud connects in plant nutrition liquid, in the plant nutrient
Liquid includes stock extracting solution；
Stock processing: selecting eugonic Xinjiang poplar carpentery workshop is stock, and bud interface is prepared on the nursery stock of Xinjiang poplar, with mixing
Liquid sprays the bud interface, and in terms of the volume of the mixed liquor, gibberellin 100-500mg/L, indoles-are contained in the mixed liquor
3- acetic acid 50-200mg/L, methyl α-naphthyl acetate 50-200mg/L, potassium dihydrogen phosphate 400-800mg/L and calcium superphosphate 500-1000mg/
L；
It grafts bud tablets: the bud tablets being inserted into the bud interface, bundle bud socket part position, obtain grafting nursery stock；
Soil treatment: soil conditioner is added in the root of the grafting nursery stock, completes grafting.
2. the engrafting method according to claim 1 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that the soil improvement
Agent, in parts by weight, material component includes:
100-150 parts of diatomite, 80-120 parts of flyash, 70-90 parts of bentonite, 50-70 parts of kaolin, 80-120 parts of turf,
120-160 parts of plant straw powder, -20 parts of carrier 12 containing algae, 40-60 parts of organic fertilizer, 40-70 parts of humus, zeolite powder 20-35
Part, 20-30 parts of glass microballoon, 18-25 parts of Cab-O-sil, 3-5 parts of hydrophobic resin, 20-30 parts of microbial inoculum；
10-20 parts of POLYPROPYLENE GLYCOL, 10-15 parts of urea, 8-10 parts of monoammonium phosphate, 30-40 parts of waste residue of Chinese herbs powder, useless tea grounds 20-40
Part, 8-24 parts of fulvic acid concentrate, 40-60 parts of vinasse, 30-60 parts of leaf mould, 40-60 parts of sawdust, 20-30 parts of rice chaff ash, pine
10-30 parts of phosphorus, 50-70 parts of coco bran and 30-40 parts of slow-release fertilizer.
3. the engrafting method according to claim 2 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that contain in the mixed liquor
There are gibberellin 200-400mg/L, indole-3-acetic acid 100-150mg/L, methyl α-naphthyl acetate 100-120mg/L, potassium dihydrogen phosphate 500-
600mg/L and calcium superphosphate 700-800mg/L.
4. the engrafting method according to claim 3 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that the soil conditioner
It is made by the following steps:
1), kaolin and flyash are uniformly mixed, and are added that water stirring is agglomerating, and dough heats under the conditions of being placed in 30-35 DEG C
1-1.5 hours, obtain the first mixture；
2) fulvic acid concentrate is added after, mixing diatomite and turf, adds water and continues to mix, obtain the second mixture；
3), by plant straw powder be added straw decomposing inoculant it is decomposed after mix with organic fertilizer, humus, waste residue of Chinese herbs powder and useless tea grounds
It closes, and mixes after coco bran mixes and mix thoroughly with vinasse, leaf mould, sawdust, rice chaff ash and loose phosphorus after heap fermentation, and in 20-30 DEG C
2-3 days are stood, third mixture is obtained；
4) hydrophobic resin is added after, mixing zeolite powder, glass microballoon and Cab-O-sil to stir evenly；Obtain the 4th mixture；
5) urea, monoammonium phosphate and slow-release fertilizer is added after, mixing second mixture, the third mixture, and carries out
Dispersion stirring, then is added first mixture and the 4th mixture thereto, be stirring evenly and then adding into carrier containing algae with
And microbial inoculum, continue under paving after mixing to 25-30 DEG C of environment 2-5 hours, obtains pre- using powder；
6) inorganic salts that quality is its 2-4 times water and quality is its 1%-4%, agitated or extruding, is added in bentonite
It is modified, then be classified and purified, obtain slurries of the granularity 90% less than 10 μm；By the slurries and have been dissolved in the poly- of water
Propylene alcohol solution, water carry out mixture, and are individually encapsulated after mixing evenly with 20-30 revs/min of revolving speed, obtain pre- using liquid
Material.
5. the engrafting method according to claim 4 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that the bud tablets are immersed in
Ultrasonication is also carried out when in plant nutrition liquid.
6. the engrafting method according to claim 5 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that the power of ultrasonic wave is
100-200W, each ultrasound 5-8s, it is adjacent it is ultrasonic twice between be divided into 20-30s.
7. the engrafting method according to claim 1-6 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that the bud
Piece band xylem, 1-3 centimetres of the lower end ion leaf of the bud interface.
8. the engrafting method according to claim 1-6 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that the system
Standby bud interface specifically:
Xinjiang poplar nursery stock stem output on one side a length of 2-3 centimetres, width be 2/5-3/5 stem week bud interface；
Water cannot be poured in 10 days after grafting and pour bud socket part position.
9. the engrafting method according to claim 1-6 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that the bundle
It ties up are as follows: bud tablets are fixed on bud interface, are bundled from bottom to top with bundling belt.
10. the engrafting method according to claim 1-6 for promoting diversiform-leaved poplar vegetative propagation, which is characterized in that described
Stock extracting solution has the following steps to be made:
The water of the rhizome of Xinjiang poplar, 5~10 times of strange willow rhizome quality is subjected to 1~2h of hyperbaric heating, be obtained by filtration filtrate and
Filter residue；
Filter residue and filtrate are placed in closed container, zymophyte is accessed in a reservoir and carries out 4~6h of fermentation；
Stock extracting solution is obtained by filtration after fermentation.
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