CN109072289A - 自动化巢式重组酶聚合酶扩增 - Google Patents

自动化巢式重组酶聚合酶扩增 Download PDF

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Publication number
CN109072289A
CN109072289A CN201780022595.2A CN201780022595A CN109072289A CN 109072289 A CN109072289 A CN 109072289A CN 201780022595 A CN201780022595 A CN 201780022595A CN 109072289 A CN109072289 A CN 109072289A
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nucleic acid
sample
amplification
reaction chamber
pump
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Chinese (zh)
Inventor
默里·约翰·怀特
尼尔·A·阿姆斯
奥拉夫·派普拉
凯撒利尔·J·格林伍德
O·南特维奇
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Abbott Diagnostics Scarborough Inc
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • B01L2300/06—Auxiliary integrated devices, integrated components
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201780022595.2A 2016-03-04 2017-03-03 自动化巢式重组酶聚合酶扩增 Pending CN109072289A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662303934P 2016-03-04 2016-03-04
US62/303,934 2016-03-04
PCT/US2017/020782 WO2017152122A2 (en) 2016-03-04 2017-03-03 Automated nested recombinase polymerase amplification

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CN109072289A true CN109072289A (zh) 2018-12-21

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US (2) US12195813B2 (enExample)
EP (2) EP3423599B1 (enExample)
JP (3) JP2019508043A (enExample)
KR (2) KR20220167397A (enExample)
CN (1) CN109072289A (enExample)
AU (1) AU2017228401B2 (enExample)
BR (1) BR112018067739A2 (enExample)
CA (2) CA3268059A1 (enExample)
ES (1) ES2950618T3 (enExample)
MX (1) MX2018010575A (enExample)
WO (1) WO2017152122A2 (enExample)

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CN111235316A (zh) * 2020-03-23 2020-06-05 中国检验检疫科学研究院 鉴定新型冠状病毒的引物探针及在三重荧光rpa的应用
CN111621597A (zh) * 2020-05-09 2020-09-04 清华大学 一种病毒重组酶-聚合酶扩增检测方法
CN112501262A (zh) * 2020-12-15 2021-03-16 苏州点晶生物科技有限公司 一种基于双重多酶介导的核酸扩增检测方法及试剂
CN112739833A (zh) * 2020-04-25 2021-04-30 黄婉秋 利用巢式RPA技术检测SARS-CoV-2的引物对、探针、试剂盒及其应用
CN112899404A (zh) * 2021-03-16 2021-06-04 广东省农业科学院动物卫生研究所 鳜蛙虹彩病毒的Nested-RAA检测引物对及其应用和检测方法
WO2021226754A1 (zh) * 2020-05-09 2021-11-18 杭州梓晶生物有限公司 一种病毒重组酶-聚合酶扩增检测方法
WO2022174377A1 (zh) * 2021-02-19 2022-08-25 清华大学 一种巢式重组酶-聚合酶扩增的方法及其应用

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USD834724S1 (en) * 2016-10-14 2018-11-27 Life Technologies Corporation Illumination and imaging instrument
JP7311420B2 (ja) 2017-02-28 2023-07-19 アボット・ダイアグノスティックス・スカボロー・インコーポレイテッド マイクロ流体デバイスおよび関連する方法
CN108103247A (zh) * 2018-02-01 2018-06-01 上海海洋大学 基于rpa技术检测ⅲ型草鱼呼肠孤病毒的试剂盒及其专用引物和探针
USD909605S1 (en) * 2019-01-21 2021-02-02 Life Technologies Corporation Polymerase chain reaction instrument
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CN112481358A (zh) * 2020-11-11 2021-03-12 清华大学 一种巢式重组酶-聚合酶扩增方法及其应用
CN112760417B (zh) * 2021-01-20 2022-05-17 中国疾病预防控制中心病毒病预防控制所 一种rap基因检测试剂盒、检测方法和应用及rap病毒检测试剂盒
EP4433213A1 (en) * 2021-11-17 2024-09-25 LEX Diagnostics Ltd Devices, systems, and methods for nucleic acid amplification
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US12612667B1 (en) 2023-05-01 2026-04-28 National Technology & Engineering Solutions Of Sandia, Llc Solid phase recombinase polymerase amplification for human pathogen identification
WO2025117245A1 (en) * 2023-11-30 2025-06-05 Idexx Laboratories, Inc. Point-of-care analyzers including a sample preparation tray

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