KR20220167397A - 자동화된 네스티드 재조합효소 중합효소 증폭 - Google Patents

자동화된 네스티드 재조합효소 중합효소 증폭 Download PDF

Info

Publication number
KR20220167397A
KR20220167397A KR1020227042058A KR20227042058A KR20220167397A KR 20220167397 A KR20220167397 A KR 20220167397A KR 1020227042058 A KR1020227042058 A KR 1020227042058A KR 20227042058 A KR20227042058 A KR 20227042058A KR 20220167397 A KR20220167397 A KR 20220167397A
Authority
KR
South Korea
Prior art keywords
sample
nucleic acid
module
amplification
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020227042058A
Other languages
English (en)
Korean (ko)
Inventor
머레이 존 화이트
니얼 에이. 아르메스
올라프 피펜버그
올리버 닌트위치
캐서린 진 그린우드
Original Assignee
애보트 다이어그노스틱스 스카보로, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 애보트 다이어그노스틱스 스카보로, 인크. filed Critical 애보트 다이어그노스틱스 스카보로, 인크.
Publication of KR20220167397A publication Critical patent/KR20220167397A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020227042058A 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭 Ceased KR20220167397A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662303934P 2016-03-04 2016-03-04
US62/303,934 2016-03-04
PCT/US2017/020782 WO2017152122A2 (en) 2016-03-04 2017-03-03 Automated nested recombinase polymerase amplification
KR1020187028595A KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020187028595A Division KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭

Publications (1)

Publication Number Publication Date
KR20220167397A true KR20220167397A (ko) 2022-12-20

Family

ID=59744470

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020227042058A Ceased KR20220167397A (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭
KR1020187028595A Active KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020187028595A Active KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭

Country Status (11)

Country Link
US (2) US12195813B2 (enExample)
EP (2) EP3423599B1 (enExample)
JP (3) JP2019508043A (enExample)
KR (2) KR20220167397A (enExample)
CN (1) CN109072289A (enExample)
AU (1) AU2017228401B2 (enExample)
BR (1) BR112018067739A2 (enExample)
CA (2) CA3268059A1 (enExample)
ES (1) ES2950618T3 (enExample)
MX (1) MX2018010575A (enExample)
WO (1) WO2017152122A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
USD834724S1 (en) * 2016-10-14 2018-11-27 Life Technologies Corporation Illumination and imaging instrument
JP7311420B2 (ja) 2017-02-28 2023-07-19 アボット・ダイアグノスティックス・スカボロー・インコーポレイテッド マイクロ流体デバイスおよび関連する方法
CN108103247A (zh) * 2018-02-01 2018-06-01 上海海洋大学 基于rpa技术检测ⅲ型草鱼呼肠孤病毒的试剂盒及其专用引物和探针
USD909605S1 (en) * 2019-01-21 2021-02-02 Life Technologies Corporation Polymerase chain reaction instrument
CN111235316B (zh) * 2020-03-23 2022-11-25 中国检验检疫科学研究院 鉴定新型冠状病毒的引物探针及在三重荧光rpa的应用
CN112739833B (zh) * 2020-04-25 2024-08-30 黄婉秋 利用巢式RPA技术检测SARS-CoV-2的引物对、探针、试剂盒及其应用
WO2021226754A1 (zh) * 2020-05-09 2021-11-18 杭州梓晶生物有限公司 一种病毒重组酶-聚合酶扩增检测方法
CN111621597A (zh) * 2020-05-09 2020-09-04 清华大学 一种病毒重组酶-聚合酶扩增检测方法
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe
CN112481358A (zh) * 2020-11-11 2021-03-12 清华大学 一种巢式重组酶-聚合酶扩增方法及其应用
CN112501262A (zh) * 2020-12-15 2021-03-16 苏州点晶生物科技有限公司 一种基于双重多酶介导的核酸扩增检测方法及试剂
CN112760417B (zh) * 2021-01-20 2022-05-17 中国疾病预防控制中心病毒病预防控制所 一种rap基因检测试剂盒、检测方法和应用及rap病毒检测试剂盒
WO2022174377A1 (zh) * 2021-02-19 2022-08-25 清华大学 一种巢式重组酶-聚合酶扩增的方法及其应用
CN112899404B (zh) * 2021-03-16 2022-12-27 广东省农业科学院动物卫生研究所 鳜蛙虹彩病毒的Nested-RAA检测引物对及其应用和检测方法
EP4433213A1 (en) * 2021-11-17 2024-09-25 LEX Diagnostics Ltd Devices, systems, and methods for nucleic acid amplification
WO2024064317A2 (en) * 2022-09-23 2024-03-28 Customarray Inc. Reagent delivery system with fluidic device and sensor
US12612667B1 (en) 2023-05-01 2026-04-28 National Technology & Engineering Solutions Of Sandia, Llc Solid phase recombinase polymerase amplification for human pathogen identification
WO2025117245A1 (en) * 2023-11-30 2025-06-05 Idexx Laboratories, Inc. Point-of-care analyzers including a sample preparation tray

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7183052B2 (en) * 2001-07-17 2007-02-27 Stratagene California Methods for detection of a target nucleic acid using multi-subunit probes
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
CA2476481C (en) 2002-02-21 2016-01-26 Asm Scientific, Inc. Recombinase polymerase amplification
EP3985125A1 (en) 2004-06-01 2022-04-20 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
CN101068932B (zh) 2004-10-27 2013-02-13 塞弗德公司 封闭系统多阶段核酸扩增反应
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
WO2006099042A2 (en) * 2005-03-09 2006-09-21 The Regents Of The University Of California Microfluidic valve for liquids
EP1929049B1 (en) 2005-07-25 2013-04-10 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
EP2360281B1 (en) * 2006-02-13 2013-07-03 The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services Primers and probes for detection and discrimination of types and subtypes of influenza viruses
JP5254949B2 (ja) * 2006-03-15 2013-08-07 マイクロニクス， インコーポレイテッド 一体型の核酸アッセイ
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド レコンビナーゼポリメラーゼ増幅
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP2326732A4 (en) * 2008-08-26 2012-11-14 Fluidigm Corp TEST METHODS FOR INCREASED SURPLUS OF SAMPLES AND / OR TARGETS
US9469867B2 (en) * 2009-05-20 2016-10-18 Alere San Diego, Inc. DNA glycosylase/lyase and AP endonuclease substrates
EP3360974B1 (en) 2009-06-05 2025-04-09 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification reagents
US9334529B2 (en) * 2009-11-03 2016-05-10 Leijon Diagnostics Ab Genotyping of N loci in a target nucleic acid molecule
WO2011100749A2 (en) * 2010-02-15 2011-08-18 Cascade Biosystems, Inc. Methods and materials for detecting viral or microbial infections
EP3978620A1 (en) * 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
EP2994543B1 (en) * 2013-05-07 2018-08-15 Micronics, Inc. Device for preparation and analysis of nucleic acids
GB2516675A (en) * 2013-07-29 2015-02-04 Atlas Genetics Ltd A valve which depressurises, and a valve system
US9567645B2 (en) * 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
CN104195236B (zh) * 2014-08-13 2015-08-19 北京恩济和生物科技有限公司 一种巢式pcr数字定量方法
WO2017147486A1 (en) * 2016-02-26 2017-08-31 Alere San Diego Inc. Redox labeled oligonucleotide probes and their use
JP7311420B2 (ja) * 2017-02-28 2023-07-19 アボット・ダイアグノスティックス・スカボロー・インコーポレイテッド マイクロ流体デバイスおよび関連する方法

Also Published As

Publication number Publication date
US12195813B2 (en) 2025-01-14
KR20180133406A (ko) 2018-12-14
CA3016582A1 (en) 2017-09-08
WO2017152122A3 (en) 2017-10-19
KR102473905B1 (ko) 2022-12-06
CA3268059A1 (en) 2026-03-02
JP2021118695A (ja) 2021-08-12
EP3423599A2 (en) 2019-01-09
EP4275794A2 (en) 2023-11-15
CN109072289A (zh) 2018-12-21
BR112018067739A2 (pt) 2019-01-08
ES2950618T3 (es) 2023-10-11
US20250382677A1 (en) 2025-12-18
WO2017152122A2 (en) 2017-09-08
MX2018010575A (es) 2019-01-10
AU2017228401A1 (en) 2018-09-27
JP2019508043A (ja) 2019-03-28
JP2023123447A (ja) 2023-09-05
EP4275794A3 (en) 2024-01-17
EP3423599B1 (en) 2023-04-19
US20200232050A1 (en) 2020-07-23
AU2017228401B2 (en) 2023-07-27
EP3423599A4 (en) 2020-01-08

Similar Documents

Publication Publication Date Title
KR102473905B1 (ko) 자동화된 네스티드 재조합효소 중합효소 증폭
US20240359180A1 (en) Devices, systems, and methods for analysis of nucleic acids
JP6285478B2 (ja) 一体化された試料調製、反応、及び検出のための装置及び方法
US20190212295A1 (en) Systems and Devices for High-Throughput Sequencing with Semiconductor-Based Detection
US10544456B2 (en) Systems and methods for nucleic acid sequencing
US11953464B2 (en) Semiconductor-based biosensors for base calling
CN109996860A (zh) 用于核酸制备的系统
WO2020142739A1 (en) COMPOSITIONS AND METHODS FOR DETECTING MODIFIED NUCLEIC ACIDS AND AMPLIFYING ssDNA
CN110088293A (zh) 分子载入样品孔以供分析
US20240209421A1 (en) Single-buffer compositions for nucleic acid detection
CN101151370A (zh) 反应容器、反应容器处理装置及诊断装置
HK40102799A (en) Device for automated nested recombinase polymerase amplification
US20260085339A1 (en) Pathogen testing device and method
WO2025034518A1 (en) Instrumentation and methods for analysis of nucleic acids
BR112019027625B1 (pt) Biossensor para chamada de base, dispositivo com um receptáculo retendo este, e método para chamada de base
BR112019007053B1 (pt) Sensores tendo conjunto de circuitos de proteção integrada
HK40005955A (en) Sensors having integrated protection circuitry

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20221130

Application number text: 1020187028595

Filing date: 20181002

PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20230711

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20240117

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20230711

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I