KR20220167397A - 자동화된 네스티드 재조합효소 중합효소 증폭 - Google Patents

자동화된 네스티드 재조합효소 중합효소 증폭 Download PDF

Info

Publication number
KR20220167397A
KR20220167397A KR1020227042058A KR20227042058A KR20220167397A KR 20220167397 A KR20220167397 A KR 20220167397A KR 1020227042058 A KR1020227042058 A KR 1020227042058A KR 20227042058 A KR20227042058 A KR 20227042058A KR 20220167397 A KR20220167397 A KR 20220167397A
Authority
KR
South Korea
Prior art keywords
sample
nucleic acid
module
amplification
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020227042058A
Other languages
English (en)
Korean (ko)
Inventor
머레이 존 화이트
니얼 에이. 아르메스
올라프 피펜버그
올리버 닌트위치
캐서린 진 그린우드
Original Assignee
애보트 다이어그노스틱스 스카보로, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 애보트 다이어그노스틱스 스카보로, 인크. filed Critical 애보트 다이어그노스틱스 스카보로, 인크.
Publication of KR20220167397A publication Critical patent/KR20220167397A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
KR1020227042058A 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭 Ceased KR20220167397A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662303934P 2016-03-04 2016-03-04
US62/303,934 2016-03-04
KR1020187028595A KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭
PCT/US2017/020782 WO2017152122A2 (en) 2016-03-04 2017-03-03 Automated nested recombinase polymerase amplification

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020187028595A Division KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭

Publications (1)

Publication Number Publication Date
KR20220167397A true KR20220167397A (ko) 2022-12-20

Family

ID=59744470

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020227042058A Ceased KR20220167397A (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭
KR1020187028595A Active KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020187028595A Active KR102473905B1 (ko) 2016-03-04 2017-03-03 자동화된 네스티드 재조합효소 중합효소 증폭

Country Status (11)

Country Link
US (2) US12195813B2 (enExample)
EP (2) EP4275794A3 (enExample)
JP (3) JP2019508043A (enExample)
KR (2) KR20220167397A (enExample)
CN (1) CN109072289A (enExample)
AU (1) AU2017228401B2 (enExample)
BR (1) BR112018067739A2 (enExample)
CA (2) CA3268059A1 (enExample)
ES (1) ES2950618T3 (enExample)
MX (1) MX2018010575A (enExample)
WO (1) WO2017152122A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
USD834724S1 (en) * 2016-10-14 2018-11-27 Life Technologies Corporation Illumination and imaging instrument
US10632464B2 (en) * 2017-02-28 2020-04-28 Alere San Diego, Inc. Microfluidic devices and related methods
CN108103247A (zh) * 2018-02-01 2018-06-01 上海海洋大学 基于rpa技术检测ⅲ型草鱼呼肠孤病毒的试剂盒及其专用引物和探针
USD909605S1 (en) * 2019-01-21 2021-02-02 Life Technologies Corporation Polymerase chain reaction instrument
CN111235316B (zh) * 2020-03-23 2022-11-25 中国检验检疫科学研究院 鉴定新型冠状病毒的引物探针及在三重荧光rpa的应用
CN112739833B (zh) * 2020-04-25 2024-08-30 黄婉秋 利用巢式RPA技术检测SARS-CoV-2的引物对、探针、试剂盒及其应用
CN111621597A (zh) * 2020-05-09 2020-09-04 清华大学 一种病毒重组酶-聚合酶扩增检测方法
WO2021226754A1 (zh) * 2020-05-09 2021-11-18 杭州梓晶生物有限公司 一种病毒重组酶-聚合酶扩增检测方法
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe
CN112481358A (zh) * 2020-11-11 2021-03-12 清华大学 一种巢式重组酶-聚合酶扩增方法及其应用
CN112501262A (zh) * 2020-12-15 2021-03-16 苏州点晶生物科技有限公司 一种基于双重多酶介导的核酸扩增检测方法及试剂
CN112760417B (zh) * 2021-01-20 2022-05-17 中国疾病预防控制中心病毒病预防控制所 一种rap基因检测试剂盒、检测方法和应用及rap病毒检测试剂盒
WO2022174377A1 (zh) * 2021-02-19 2022-08-25 清华大学 一种巢式重组酶-聚合酶扩增的方法及其应用
CN112899404B (zh) * 2021-03-16 2022-12-27 广东省农业科学院动物卫生研究所 鳜蛙虹彩病毒的Nested-RAA检测引物对及其应用和检测方法
WO2023088952A1 (en) * 2021-11-17 2023-05-25 Lex diagnostics Ltd. Devices, systems, and methods for nucleic acid amplification
WO2024064317A2 (en) * 2022-09-23 2024-03-28 Customarray Inc. Reagent delivery system with fluidic device and sensor
US12612667B1 (en) 2023-05-01 2026-04-28 National Technology & Engineering Solutions Of Sandia, Llc Solid phase recombinase polymerase amplification for human pathogen identification
WO2025117245A1 (en) * 2023-11-30 2025-06-05 Idexx Laboratories, Inc. Point-of-care analyzers including a sample preparation tray

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002365028A1 (en) * 2001-07-17 2003-06-30 Stratagene Methods for detection of a target nucleic acid using multi-subunit probes
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2005118853A2 (en) 2004-06-01 2005-12-15 Asm Scientific, Inc. Recombinase polymerase amplification
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
CA2485653A1 (en) 2002-05-31 2003-12-11 Janssen Pharmaceutica N.V. Human 5-ht7 receptor promoter sequence
US7544506B2 (en) 2003-06-06 2009-06-09 Micronics, Inc. System and method for heating, cooling and heat cycling on microfluidic device
US8133703B2 (en) * 2004-10-27 2012-03-13 Ceoheid Closed-system multi-stage nucleic acid amplification reactions
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
JP2008532748A (ja) * 2005-03-09 2008-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 液体用マイクロ流体バルブ
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
SG10201806098QA (en) * 2006-02-13 2018-08-30 Us Gov Health & Human Services Primers and probes for detection and discrimination of types and subtypes of influenza viruses
JP5254949B2 (ja) * 2006-03-15 2013-08-07 マイクロニクス， インコーポレイテッド 一体型の核酸アッセイ
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2010027870A2 (en) * 2008-08-26 2010-03-11 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
CH699853A1 (de) 2008-11-13 2010-05-14 Tecan Trading Ag Messgerät und Verfahren zum Bestimmen von durch ein Laborsystem bereitgestellten Fluidparametern.
WO2010059626A1 (en) 2008-11-18 2010-05-27 Danisco Us Inc. Filamentous fungi with impaired ptrb activity for altered protein production
JP5847076B2 (ja) * 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2011056133A1 (en) * 2009-11-03 2011-05-12 Sva Statens Veterinärmedicinska Anstalt Genotyping
US8597886B2 (en) * 2010-02-15 2013-12-03 Cascade Biosystems, Inc. Methods and materials for detecting viral or microbial infections
EP3978620A1 (en) * 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
CN105189784B (zh) * 2013-05-07 2020-06-30 珀金埃尔默健康科学有限公司 用于制备和分析核酸的装置
GB2516675A (en) * 2013-07-29 2015-02-04 Atlas Genetics Ltd A valve which depressurises, and a valve system
CN105705659B (zh) * 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
CN104195236B (zh) * 2014-08-13 2015-08-19 北京恩济和生物科技有限公司 一种巢式pcr数字定量方法
ES3032616T3 (en) * 2016-02-26 2025-07-22 Abbott Diagnostics Scarborough Inc Redox labeled oligonucleotide probes and their use
US10632464B2 (en) * 2017-02-28 2020-04-28 Alere San Diego, Inc. Microfluidic devices and related methods

Also Published As

Publication number Publication date
WO2017152122A2 (en) 2017-09-08
EP4275794A2 (en) 2023-11-15
CN109072289A (zh) 2018-12-21
KR20180133406A (ko) 2018-12-14
US20200232050A1 (en) 2020-07-23
JP7867767B2 (ja) 2026-06-01
ES2950618T3 (es) 2023-10-11
EP3423599A4 (en) 2020-01-08
MX2018010575A (es) 2019-01-10
EP3423599A2 (en) 2019-01-09
US20250382677A1 (en) 2025-12-18
US12195813B2 (en) 2025-01-14
WO2017152122A3 (en) 2017-10-19
JP2023123447A (ja) 2023-09-05
EP3423599B1 (en) 2023-04-19
JP2021118695A (ja) 2021-08-12
AU2017228401A1 (en) 2018-09-27
BR112018067739A2 (pt) 2019-01-08
CA3268059A1 (en) 2026-03-02
AU2017228401B2 (en) 2023-07-27
KR102473905B1 (ko) 2022-12-06
JP2019508043A (ja) 2019-03-28
EP4275794A3 (en) 2024-01-17
CA3016582A1 (en) 2017-09-08

Similar Documents

Publication Publication Date Title
KR102473905B1 (ko) 자동화된 네스티드 재조합효소 중합효소 증폭
US20240359180A1 (en) Devices, systems, and methods for analysis of nucleic acids
JP6285478B2 (ja) 一体化された試料調製、反応、及び検出のための装置及び方法
US20190212295A1 (en) Systems and Devices for High-Throughput Sequencing with Semiconductor-Based Detection
US10544456B2 (en) Systems and methods for nucleic acid sequencing
US11953464B2 (en) Semiconductor-based biosensors for base calling
CN109996860A (zh) 用于核酸制备的系统
WO2020142739A1 (en) COMPOSITIONS AND METHODS FOR DETECTING MODIFIED NUCLEIC ACIDS AND AMPLIFYING ssDNA
CN110088293A (zh) 分子载入样品孔以供分析
US20240209421A1 (en) Single-buffer compositions for nucleic acid detection
CN101151370A (zh) 反应容器、反应容器处理装置及诊断装置
HK40102799A (en) Device for automated nested recombinase polymerase amplification
US20260085339A1 (en) Pathogen testing device and method
WO2025034518A1 (en) Instrumentation and methods for analysis of nucleic acids
BR112019027625B1 (pt) Biossensor para chamada de base, dispositivo com um receptáculo retendo este, e método para chamada de base
BR112019007053B1 (pt) Sensores tendo conjunto de circuitos de proteção integrada
HK40005955A (en) Sensors having integrated protection circuitry

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20221130

Application number text: 1020187028595

Filing date: 20181002

PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20230711

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20240117

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20230711

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I