JP2019508043A - 自動化ネステッドリコンビナーゼポリメラーゼ増幅 - Google Patents

自動化ネステッドリコンビナーゼポリメラーゼ増幅 Download PDF

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JP2019508043A
JP2019508043A JP2018546635A JP2018546635A JP2019508043A JP 2019508043 A JP2019508043 A JP 2019508043A JP 2018546635 A JP2018546635 A JP 2018546635A JP 2018546635 A JP2018546635 A JP 2018546635A JP 2019508043 A JP2019508043 A JP 2019508043A
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nucleic acid
amplification
sample
reaction chamber
acid amplification
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JP2019508043A5 (enExample
Inventor
ジョン ホワイテ，ムレー
ジョン ホワイテ，ムレー
エー． アルメス，ナイアル
エー． アルメス，ナイアル
パイペンブルグ，オラフ
ジーン グリーンウッヅ，キャサリン
ジーン グリーンウッヅ，キャサリン
ネントウィック，オリバー
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アリーア サン ディエゴ， インコーポレイテッド
アリーア サン ディエゴ， インコーポレイテッド
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Publication of JP2019508043A publication Critical patent/JP2019508043A/ja
Publication of JP2019508043A5 publication Critical patent/JP2019508043A5/ja
Priority to JP2021064771A priority Critical patent/JP2021118695A/ja
Priority to JP2023088617A priority patent/JP2023123447A/ja
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
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    • B01L2300/0627—Sensor or part of a sensor is integrated
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

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  • Electrochemistry (AREA)
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  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018546635A 2016-03-04 2017-03-03 自動化ネステッドリコンビナーゼポリメラーゼ増幅 Pending JP2019508043A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021064771A JP2021118695A (ja) 2016-03-04 2021-04-06 自動化ネステッドリコンビナーゼポリメラーゼ増幅
JP2023088617A JP2023123447A (ja) 2016-03-04 2023-05-30 自動化ネステッドリコンビナーゼポリメラーゼ増幅

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662303934P 2016-03-04 2016-03-04
US62/303,934 2016-03-04
PCT/US2017/020782 WO2017152122A2 (en) 2016-03-04 2017-03-03 Automated nested recombinase polymerase amplification

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JP2021064771A Division JP2021118695A (ja) 2016-03-04 2021-04-06 自動化ネステッドリコンビナーゼポリメラーゼ増幅

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JP2019508043A true JP2019508043A (ja) 2019-03-28
JP2019508043A5 JP2019508043A5 (enExample) 2020-02-27

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JP2021064771A Pending JP2021118695A (ja) 2016-03-04 2021-04-06 自動化ネステッドリコンビナーゼポリメラーゼ増幅
JP2023088617A Pending JP2023123447A (ja) 2016-03-04 2023-05-30 自動化ネステッドリコンビナーゼポリメラーゼ増幅

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JP2023088617A Pending JP2023123447A (ja) 2016-03-04 2023-05-30 自動化ネステッドリコンビナーゼポリメラーゼ増幅

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US (2) US12195813B2 (enExample)
EP (2) EP3423599B1 (enExample)
JP (3) JP2019508043A (enExample)
KR (2) KR20220167397A (enExample)
CN (1) CN109072289A (enExample)
AU (1) AU2017228401B2 (enExample)
BR (1) BR112018067739A2 (enExample)
CA (2) CA3268059A1 (enExample)
ES (1) ES2950618T3 (enExample)
MX (1) MX2018010575A (enExample)
WO (1) WO2017152122A2 (enExample)

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CN111621597A (zh) * 2020-05-09 2020-09-04 清华大学 一种病毒重组酶-聚合酶扩增检测方法
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CN112481358A (zh) * 2020-11-11 2021-03-12 清华大学 一种巢式重组酶-聚合酶扩增方法及其应用
CN112501262A (zh) * 2020-12-15 2021-03-16 苏州点晶生物科技有限公司 一种基于双重多酶介导的核酸扩增检测方法及试剂
CN112760417B (zh) * 2021-01-20 2022-05-17 中国疾病预防控制中心病毒病预防控制所 一种rap基因检测试剂盒、检测方法和应用及rap病毒检测试剂盒
WO2022174377A1 (zh) * 2021-02-19 2022-08-25 清华大学 一种巢式重组酶-聚合酶扩增的方法及其应用
CN112899404B (zh) * 2021-03-16 2022-12-27 广东省农业科学院动物卫生研究所 鳜蛙虹彩病毒的Nested-RAA检测引物对及其应用和检测方法
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