AU2017228401B2 - Automated nested recombinase polymerase amplification - Google Patents

Automated nested recombinase polymerase amplification Download PDF

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AU2017228401B2
AU2017228401B2 AU2017228401A AU2017228401A AU2017228401B2 AU 2017228401 B2 AU2017228401 B2 AU 2017228401B2 AU 2017228401 A AU2017228401 A AU 2017228401A AU 2017228401 A AU2017228401 A AU 2017228401A AU 2017228401 B2 AU2017228401 B2 AU 2017228401B2
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sample
nucleic acid
amplification
amplification product
module
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AU2017228401A1 (en
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Niall A. Armes
Catherine Jean GREENWOOD
Oliver Nentwich
Olaf Piepenburg
Murray John WHYTE
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Abbott Diagnostics Scarborough Inc
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Abbott Diagnostics Scarborough Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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AU2017228401A 2016-03-04 2017-03-03 Automated nested recombinase polymerase amplification Active AU2017228401B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662303934P 2016-03-04 2016-03-04
US62/303,934 2016-03-04
PCT/US2017/020782 WO2017152122A2 (en) 2016-03-04 2017-03-03 Automated nested recombinase polymerase amplification

Publications (2)

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AU2017228401A1 AU2017228401A1 (en) 2018-09-27
AU2017228401B2 true AU2017228401B2 (en) 2023-07-27

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US (2) US12195813B2 (enExample)
EP (2) EP4275794A3 (enExample)
JP (3) JP2019508043A (enExample)
KR (2) KR20220167397A (enExample)
CN (1) CN109072289A (enExample)
AU (1) AU2017228401B2 (enExample)
BR (1) BR112018067739A2 (enExample)
CA (2) CA3268059A1 (enExample)
ES (1) ES2950618T3 (enExample)
MX (1) MX2018010575A (enExample)
WO (1) WO2017152122A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
USD834724S1 (en) * 2016-10-14 2018-11-27 Life Technologies Corporation Illumination and imaging instrument
US10632464B2 (en) * 2017-02-28 2020-04-28 Alere San Diego, Inc. Microfluidic devices and related methods
CN108103247A (zh) * 2018-02-01 2018-06-01 上海海洋大学 基于rpa技术检测ⅲ型草鱼呼肠孤病毒的试剂盒及其专用引物和探针
USD909605S1 (en) * 2019-01-21 2021-02-02 Life Technologies Corporation Polymerase chain reaction instrument
CN111235316B (zh) * 2020-03-23 2022-11-25 中国检验检疫科学研究院 鉴定新型冠状病毒的引物探针及在三重荧光rpa的应用
CN112739833B (zh) * 2020-04-25 2024-08-30 黄婉秋 利用巢式RPA技术检测SARS-CoV-2的引物对、探针、试剂盒及其应用
CN111621597A (zh) * 2020-05-09 2020-09-04 清华大学 一种病毒重组酶-聚合酶扩增检测方法
WO2021226754A1 (zh) * 2020-05-09 2021-11-18 杭州梓晶生物有限公司 一种病毒重组酶-聚合酶扩增检测方法
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe
CN112481358A (zh) * 2020-11-11 2021-03-12 清华大学 一种巢式重组酶-聚合酶扩增方法及其应用
CN112501262A (zh) * 2020-12-15 2021-03-16 苏州点晶生物科技有限公司 一种基于双重多酶介导的核酸扩增检测方法及试剂
CN112760417B (zh) * 2021-01-20 2022-05-17 中国疾病预防控制中心病毒病预防控制所 一种rap基因检测试剂盒、检测方法和应用及rap病毒检测试剂盒
WO2022174377A1 (zh) * 2021-02-19 2022-08-25 清华大学 一种巢式重组酶-聚合酶扩增的方法及其应用
CN112899404B (zh) * 2021-03-16 2022-12-27 广东省农业科学院动物卫生研究所 鳜蛙虹彩病毒的Nested-RAA检测引物对及其应用和检测方法
WO2023088952A1 (en) * 2021-11-17 2023-05-25 Lex diagnostics Ltd. Devices, systems, and methods for nucleic acid amplification
WO2024064317A2 (en) * 2022-09-23 2024-03-28 Customarray Inc. Reagent delivery system with fluidic device and sensor
US12612667B1 (en) 2023-05-01 2026-04-28 National Technology & Engineering Solutions Of Sandia, Llc Solid phase recombinase polymerase amplification for human pathogen identification
WO2025117245A1 (en) * 2023-11-30 2025-06-05 Idexx Laboratories, Inc. Point-of-care analyzers including a sample preparation tray

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120244534A1 (en) * 2004-10-27 2012-09-27 Cephied Closed-system multi-stage nucleic acid amplification reactions

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002365028A1 (en) * 2001-07-17 2003-06-30 Stratagene Methods for detection of a target nucleic acid using multi-subunit probes
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2005118853A2 (en) 2004-06-01 2005-12-15 Asm Scientific, Inc. Recombinase polymerase amplification
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
CA2485653A1 (en) 2002-05-31 2003-12-11 Janssen Pharmaceutica N.V. Human 5-ht7 receptor promoter sequence
US7544506B2 (en) 2003-06-06 2009-06-09 Micronics, Inc. System and method for heating, cooling and heat cycling on microfluidic device
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
JP2008532748A (ja) * 2005-03-09 2008-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 液体用マイクロ流体バルブ
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
SG10201806098QA (en) * 2006-02-13 2018-08-30 Us Gov Health & Human Services Primers and probes for detection and discrimination of types and subtypes of influenza viruses
JP5254949B2 (ja) * 2006-03-15 2013-08-07 マイクロニクス， インコーポレイテッド 一体型の核酸アッセイ
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2010027870A2 (en) * 2008-08-26 2010-03-11 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
CH699853A1 (de) 2008-11-13 2010-05-14 Tecan Trading Ag Messgerät und Verfahren zum Bestimmen von durch ein Laborsystem bereitgestellten Fluidparametern.
WO2010059626A1 (en) 2008-11-18 2010-05-27 Danisco Us Inc. Filamentous fungi with impaired ptrb activity for altered protein production
JP5847076B2 (ja) * 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2011056133A1 (en) * 2009-11-03 2011-05-12 Sva Statens Veterinärmedicinska Anstalt Genotyping
US8597886B2 (en) * 2010-02-15 2013-12-03 Cascade Biosystems, Inc. Methods and materials for detecting viral or microbial infections
EP3978620A1 (en) * 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
CN105189784B (zh) * 2013-05-07 2020-06-30 珀金埃尔默健康科学有限公司 用于制备和分析核酸的装置
GB2516675A (en) * 2013-07-29 2015-02-04 Atlas Genetics Ltd A valve which depressurises, and a valve system
CN105705659B (zh) * 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
CN104195236B (zh) * 2014-08-13 2015-08-19 北京恩济和生物科技有限公司 一种巢式pcr数字定量方法
ES3032616T3 (en) * 2016-02-26 2025-07-22 Abbott Diagnostics Scarborough Inc Redox labeled oligonucleotide probes and their use
US10632464B2 (en) * 2017-02-28 2020-04-28 Alere San Diego, Inc. Microfluidic devices and related methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120244534A1 (en) * 2004-10-27 2012-09-27 Cephied Closed-system multi-stage nucleic acid amplification reactions

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