CN109041850A - A kind of cryptomeria cuttage breeding method - Google Patents
A kind of cryptomeria cuttage breeding method Download PDFInfo
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- CN109041850A CN109041850A CN201811113122.6A CN201811113122A CN109041850A CN 109041850 A CN109041850 A CN 109041850A CN 201811113122 A CN201811113122 A CN 201811113122A CN 109041850 A CN109041850 A CN 109041850A
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- cryptomeria
- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- Environmental Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
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Abstract
The invention discloses a kind of cryptomeria cuttage breeding methods, comprising: 1) Pretreatment；2) exogenous hormone NAA50~150ppm+IBA100~300ppm is used, 0.5~1.5h of cuttings is impregnated；3) cuttage；4) it is managed after cuttage.Cryptomeria cuttage breeding method of the invention, test confirms, using exogenous hormone NAA150ppm+IBA100ppm, under the conditions of immersion cuttings 1.5h, cryptomeria cutting plantation is up to 98.33%, while improving cuttage survival rate and root growth can be promoted, method simple possible provides reliable nursery stock source to build cryptomeria fast-growing, high-yield woods.
Description
Technical field
The invention belongs to plant cuttage technical fields, and in particular to a kind of cryptomeria cuttage breeding method.
Background technique
Cryptomeria is a category in Taxodiaceae.Plant classification scholar thinks that two kinds, i.e. cryptomeria Cryptomeria can be divided into
(Cryptomeria fortunei) and Japanese cedar (C.japonica).Cryptomeria growing height can achieve 40m or so, the diameter of a cross-section of a tree trunk 1.3 meters above the ground
It is more up to 2m.Cryptomeria is aiphyllium, also has cultivation in provinces such as South of Jiangsu Province, Zhejiang, Henan, Jiangsu, Shandong, Anhui, and
Cryptomeria grows fine.The height that cryptomeria generally grows is: the leeward area without shade of height above sea level 400m~1400m, often with it is some wealthy
Leaf tree species such as castanopsis caudata (Castanopsis eyrei Tutch.), evergreen chinquapin class etc. carry out mixed life, it is desirable that annual precipitation up to 1000mm with
On, 14~19 DEG C of average temperature of the whole year, 0 DEG C of temperature on average of January or more.The cryptomeria speed of growth is fast, and material is light and soft, texture straight,
Intensity is high, and corrosion resistance is strong, and glueability is good, can be widely used for building construction, shipbuilding, bridge, plate, furniture and farm implements
It builds, there is extensive demand on the market.In addition, cryptomeria is tree-like taller and bigger, the grander grace of tree performance is not only excellent
Good Scenery of gardens tree species, and the cryptomeria of per hectare can absorb the CO2 of 60kg daily, be conducive to the purification of air, Ke Yigai
Kind environmental sanitation.
The method that the breeding of cryptomeria mostly uses conventional breeding greatly, mainly based on seed propagation and cutting propagation.With existing
For the fast development of biotechnology, the cultivation of cryptomeria resource is gradually carried out using Plant Tissue Breeding fast breeding technique.Cryptomeria fortunei Seeds
Drilling is carried out using seedbed in spring in next year after harvesting, seed disinfection and presoaking and germinating processing are carried out before seed sowing,
It germinates within after planting 20 days or so.The cuttage root-taking of plant is one and its complicated process, the cuttings of Different Provenances, different parts
Adopt fringe, different substrates, season and exogeneous growth regulator type and concentration are all the key factors for influencing cuttage rooting.Willow
The cutting medium of China fir is with vermiculite: perlite: river sand=1:2:2 ratio is best, the sponginess of the matrix because matching formation thus
It is stronger with ventilating drainage, plant cuttings base portion be less prone to it is rotten, blue He Sheng etc. (1996) also in an experiment comparison discovery willow
China fir cuttage in the sand ineffective.Cai Yankang etc. (2003) studies the cuttage raise seedling technique of cryptomeria, it is intended to logical
Cuttage and seedling culture processing experiment is crossed, the processing scheme for helping to improve cryptomeria cuttage survival rate and promoting Seedling height growth is searched out, it is real
Test the result shows that: 1-2 raw length is 20cm cuttings, after impregnating 30min with 150x10-6 No. 1 ABT rooting powder solution,
Carry out cuttage in 3-4 month, the height of cuttage seeding and the survival rate of cuttage fringe bar be all it is best, the height of cuttage seeding can be grown to
The survival rate of 46.17cm, cuttage seeding can reach 86.17%.For Tan Ling (2014) in cryptomeria cuttage experiment, research finds selection
It handles the concentration of the solution of the reagent, processing taken root and has apparent influence to cryptomeria cutting experiment to the processing time of fringe bar,
After the 200mg/L solution prepared with ABT root-inducing powder impregnates 1h, the number of rooting rate, storage rate, indefinite radical in cuttage result
According to respectively 46.80%, 68.73% and 16, as a result, it has been found that the rooting rate of cuttage is relatively low compared with forefathers' cuttage result, point
Analysis reason may be cutting time selection in temperature higher season, and higher temperature affects the cuttage survival rate of nursery stock.
Summary of the invention
Goal of the invention: the deficiencies in the prior art are directed to, the object of the present invention is to provide a kind of cryptomeria cutting propagations
Method, improves cuttage survival rate and promotes root growth, provides reliable nursery stock source to build cryptomeria fast-growing, high-yield woods.
Technical solution: in order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention are as follows:
A kind of cryptomeria cuttage breeding method, steps are as follows:
1) select the cryptomeria choiceness of the good no disease and pests harm of growth conditions as elite stand, half wood of clip current year life
The branch of matter is as cuttage fringe bar, according to upper cut truncation, the method for lower cut beveling, the 20 cuttings conducts of each clone
It a bundle, is immersed in be processed in clear water；
2) exogenous hormone NAA50~150ppm+IBA100~300ppm is used, 0.5~1.5h of cuttings is impregnated；
3) when cuttage, draw hole with small wood, by 1/2-2/3 in the fringe bar insertion cutting medium of cryptomeria, after the completion of cuttage
It will be compacted around cryptomeria fringe bar with soil, uniformly sprinkled profoundly water after cuttage completion；
4) after cuttage, shaded in the top in cuttage pond using shading net, to reduce the transpiration of cryptomeria fringe bar, while being selected certainly
Dynamic spraying water spray, guarantees that cryptomeria fringe bar has enough moisture.
In step 1), cryptomeria choiceness is 011# cryptomeria clone.
In step 2), NAA150ppm+IBA100ppm impregnates cuttings 1.5h.
In step 2), NAA100ppm+IBA200ppm impregnates cuttings 1.5h.
In step 2), NAA50ppm+IBA100ppm impregnates cuttings 0.5h.
In step 2), NAA150ppm+IBA300ppm impregnates cuttings 1.0h.
The utility model has the advantages that compared with prior art, cryptomeria cuttage breeding method of the invention, test confirms, swashed using external source
Plain NAA150ppm+IBA100ppm, under the conditions of impregnating cuttings 1.5h, cryptomeria cutting plantation up to 98.33%, improve cuttage at
While motility rate and root growth can be promoted, method simple possible provides reliable nursery stock to build cryptomeria fast-growing, high-yield woods
Source.
Detailed description of the invention
Fig. 1 is the Adventitious root initiation procedure chart of cryptomeria shoot cutting；In figure, A:45d callus is preliminarily formed；B:60d
Callus expands；C:75d adventitious root and sprouting occur；D:90d Adventitious root initiation；
Specific embodiment
The present invention is described further combined with specific embodiments below.Material used in following embodiment are as follows:
The Zhejiang Province forest farm the Xi Kou 3# cryptomeria clone (Chinese cryptomeria) and 011# cryptomeria clone of the good no disease and pests harm of growth conditions
(Japanese cedar).
Embodiment 1
A kind of cryptomeria cuttage breeding method, steps are as follows:
1) the 3# cryptomeria clone and 011# cryptomeria clone for selecting the good no disease and pests harm of growth conditions are cut as elite stand
Take the branch of the semi-lignified of current year life as cuttage fringe bar, according to upper cut truncation, the method for lower cut beveling is each asexual
It is 20 cuttings as a bundle, is immersed in be processed in clear water.
2) exogenous hormone NAA150ppm+IBA100ppm is used, cuttings 1.5h is impregnated.
3) when cuttage, draw hole with small wood, by the fringe bar insertion cutting medium (vermiculite: perlite: peat soil: yellow of cryptomeria
Soil=1: 1: 2: 1) 1/2-2/3 in, it will be compacted around cryptomeria fringe bar after the completion of cuttage with soil, it is unified after cuttage completion
It sprinkles profoundly water.
4) after cuttage, shaded in the top in cuttage pond using shading net, to reduce the transpiration of cryptomeria fringe bar, while being selected certainly
Dynamic spraying water spray, guarantees that cryptomeria fringe bar has enough moisture.
As shown in Figure 1, cryptomeria cuttings initially forms callus in cuttage 45d or so, callus size is in 2-3mm, callus color
For white, it is in strumae (Figure 1A)；In cuttage 60d or so, callus is expanded, and white is partially yellow, is in granular form protrusion, close structure
(Figure 1B)；With the accumulation of internal nutriment, adventitious root is constantly formed, and length is at 2-3cm (Fig. 1 C)；The 75d after cuttage, no
Determine root largely to be formed, difference is showed between different clones, radical differs between 2-7 root, and length is 3-6cm (Fig. 1 D).75d
Cutting plantation and situation of taking root are counted, the results are shown in Table 1, cuttage item number ÷ cutting number × 100 for rooting rate (%)=take root.
1 different clones root growth situation of table
| Clone | Total root long | Total root surface area | Average diameter | Main root mean number | Rooting rate |
| 3# | 11.36cm | 6.56cm2 | 0.60mm | 2.00 | 73.33% |
| 011# | 19.90cm | 16.44cm2 | 0.68mm | 7.33 | 98.33% |
Embodiment 2
A kind of cryptomeria cuttage breeding method, steps are as follows:
1) the 3# cryptomeria clone and 011# cryptomeria clone for selecting the good no disease and pests harm of growth conditions are cut as elite stand
Take the branch of the semi-lignified of current year life as cuttage fringe bar, according to upper cut truncation, the method for lower cut beveling is each asexual
It is 20 cuttings as a bundle, is immersed in be processed in clear water.
2) exogenous hormone NAA100ppm+IBA200ppm is used, cuttings 1.5h is impregnated.
3) when cuttage, draw hole with small wood, by 1/2-2/3 in the fringe bar insertion cutting medium of cryptomeria, after the completion of cuttage
It will be compacted around cryptomeria fringe bar with soil, uniformly sprinkled profoundly water after cuttage completion.
4) after cuttage, shaded in the top in cuttage pond using shading net, to reduce the transpiration of cryptomeria fringe bar, while being selected certainly
Dynamic spraying water spray, guarantees that cryptomeria fringe bar has enough moisture.
75d counts cutting plantation, the results are shown in Table 2, cuttage item number ÷ cutting number × 100 for rooting rate (%)=take root.
The rooting rate of 2 different clones of table
Embodiment 3
A kind of cryptomeria cuttage breeding method, steps are as follows:
1) the 3# cryptomeria clone and 011# cryptomeria clone for selecting the good no disease and pests harm of growth conditions are cut as elite stand
Take the branch of the semi-lignified of current year life as cuttage fringe bar, according to upper cut truncation, the method for lower cut beveling is each asexual
It is 20 cuttings as a bundle, is immersed in be processed in clear water.
2) exogenous hormone NAA50ppm+IBA100ppm is used, cuttings 0.5h is impregnated.
3) when cuttage, draw hole with small wood, by 1/2-2/3 in the fringe bar insertion cutting medium of cryptomeria, after the completion of cuttage
It will be compacted around cryptomeria fringe bar with soil, uniformly sprinkled profoundly water after cuttage completion.
4) after cuttage, shaded in the top in cuttage pond using shading net, to reduce the transpiration of cryptomeria fringe bar, while being selected certainly
Dynamic spraying water spray, guarantees that cryptomeria fringe bar has enough moisture.
75d counts cutting plantation, the results are shown in Table 3, cuttage item number ÷ cutting number × 100 for rooting rate (%)=take root.
The rooting rate of 3 different clones of table
| Clone | Rooting rate |
| 3# | 42.50% |
| 011# | 92.50% |
Embodiment 4
A kind of cryptomeria cuttage breeding method, steps are as follows:
1) the 3# cryptomeria clone and 011# cryptomeria clone for selecting the good no disease and pests harm of growth conditions are cut as elite stand
Take the branch of the semi-lignified of current year life as cuttage fringe bar, according to upper cut truncation, the method for lower cut beveling is each asexual
It is 20 cuttings as a bundle, is immersed in be processed in clear water.
2) exogenous hormone NAA150ppm+IBA300ppm is used, cuttings 1.0h is impregnated.
3) when cuttage, draw hole with small wood, by 1/2-2/3 in the fringe bar insertion cutting medium of cryptomeria, after the completion of cuttage
It will be compacted around cryptomeria fringe bar with soil, uniformly sprinkled profoundly water after cuttage completion.
4) after cuttage, shaded in the top in cuttage pond using shading net, to reduce the transpiration of cryptomeria fringe bar, while being selected certainly
Dynamic spraying water spray, guarantees that cryptomeria fringe bar has enough moisture.
75d counts cutting plantation, the results are shown in Table 4, cuttage item number ÷ cutting number × 100 for rooting rate (%)=take root.
The rooting rate of 4 different clones of table
| Clone | Rooting rate |
| 3# | 80% |
| 011# | 90% |
Claims (6)
1. a kind of cryptomeria cuttage breeding method, which is characterized in that steps are as follows:
1) select the cryptomeria choiceness of the good no disease and pests harm of growth conditions as elite stand, the semi-lignified of clip current year life
Branch as cuttage fringe bar, according to upper cut truncation, the method for lower cut beveling, 20 cuttings of each clone are as one
Bundle, is immersed in be processed in clear water；
2) exogenous hormone NAA50~150ppm+IBA100~300ppm is used, 0.5~1.5h of cuttings is impregnated；
3) when cuttage, draw hole with small wood, it is native after the completion of cuttage by 1/2-2/3 in the fringe bar insertion cutting medium of cryptomeria
It will be compacted around cryptomeria fringe bar, uniformly sprinkled profoundly water after cuttage completion；
4) after cuttage, shaded in the top in cuttage pond using shading net, to reduce the transpiration of cryptomeria fringe bar, while selecting automatic spray
Mist water spray guarantees that cryptomeria fringe bar has enough moisture.
2. cryptomeria cuttage breeding method according to claim 1, which is characterized in that in step 1), cryptomeria choiceness
For 011# cryptomeria clone.
3. cryptomeria cuttage breeding method according to claim 1, which is characterized in that in step 2), NAA150ppm+
IBA100ppm impregnates cuttings 1.5h.
4. cryptomeria cuttage breeding method according to claim 1, which is characterized in that in step 2), NAA100ppm+
IBA200ppm impregnates cuttings 1.5h.
5. cryptomeria cuttage breeding method according to claim 1, which is characterized in that in step 2), NAA50ppm+
IBA100ppm impregnates cuttings 0.5h.
6. cryptomeria cuttage breeding method according to claim 1, which is characterized in that in step 2), NAA150ppm+
IBA300ppm impregnates cuttings 1.0h.
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| CN201810600277.6A CN108651029A (en) | 2018-06-11 | 2018-06-11 | A kind of cryptomeria cuttage breeding method |
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| CN201811113122.6A Pending CN109041850A (en) | 2018-06-11 | 2018-09-21 | A kind of cryptomeria cuttage breeding method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111707713A (en) * | 2020-06-30 | 2020-09-25 | 南京林业大学 | Method for rapidly identifying and evaluating cold resistance of cryptomeria fortunei |
Families Citing this family (1)
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| CN112673841A (en) * | 2020-12-22 | 2021-04-20 | 中南林业科技大学 | Method for promoting differentiation of male and female flowers of cryptomeria fortunei |
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| Publication number | Publication date |
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| CN108651029A (en) | 2018-10-16 |
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