CN107614108B - 使用集成阵列对核酸杂交热力学的多路分析 - Google Patents

使用集成阵列对核酸杂交热力学的多路分析 Download PDF

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CN107614108B
CN107614108B CN201680029656.3A CN201680029656A CN107614108B CN 107614108 B CN107614108 B CN 107614108B CN 201680029656 A CN201680029656 A CN 201680029656A CN 107614108 B CN107614108 B CN 107614108B
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nucleic acid
target nucleic
acid molecule
sample
signal
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CN107614108A (zh
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奥尔延·哈斯比
卡沙玛·吉拉格
阿伦·马尼卡姆
卡韦赫·米拉尼尼亚
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Insilixa Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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CN201680029656.3A 2015-03-23 2016-03-22 使用集成阵列对核酸杂交热力学的多路分析 Active CN107614108B (zh)

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Application Number Priority Date Filing Date Title
US14/665,904 US9708647B2 (en) 2015-03-23 2015-03-23 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US14/665,904 2015-03-23
PCT/US2016/023634 WO2016154227A1 (en) 2015-03-23 2016-03-22 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays

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CN107614108B true CN107614108B (zh) 2020-10-23

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US (3) US9708647B2 (https=)
EP (2) EP3274094B1 (https=)
JP (3) JP7123560B2 (https=)
CN (1) CN107614108B (https=)
GB (1) GB2555950A (https=)
WO (1) WO2016154227A1 (https=)

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US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
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US10373704B2 (en) * 2016-06-03 2019-08-06 International Business Machines Corporation Reduction of surface nucleotide hybridization by optimizing a biosensor sensing surface area
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JP2019093377A (ja) 2017-11-22 2019-06-20 株式会社エンプラス 流体チップ、流体デバイスおよびそれらの製造方法
KR20210081335A (ko) 2018-09-03 2021-07-01 비스비 메디컬, 인코포레이티드 항생제 감수성 시험을 위한 장치 및 방법
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JP2022530323A (ja) 2019-04-28 2022-06-29 ビスビュー メディカル，インコーポレイテッド デジタル検出能力および無線接続性を有する分子診断デバイス
US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
WO2023028618A1 (en) * 2021-08-27 2023-03-02 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods to determine nucleic acid conformations and uses thereof
JP2024535381A (ja) * 2021-09-23 2024-09-30 エヌシックス・テック、インコーポレイテッド 試料分析のための方法およびシステム
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