CN107278579A - A kind of fast-growing mating system of the sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border - Google Patents

A kind of fast-growing mating system of the sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border Download PDF

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Publication number
CN107278579A
CN107278579A CN201710596819.2A CN201710596819A CN107278579A CN 107278579 A CN107278579 A CN 107278579A CN 201710596819 A CN201710596819 A CN 201710596819A CN 107278579 A CN107278579 A CN 107278579A
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rhizome
magnificent
paris polyphylla
sub
seedling
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CN107278579B (en
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陈铁柱
刘建辉
薛丹
胡平
夏燕莉
周先建
张美�
杨玉霞
郭俊霞
方清茂
张�浩
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Sichuan Academy of Chinese Medicine Sciences SACMS
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Sichuan Academy of Chinese Medicine Sciences SACMS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The present invention relates to magnificent Paris polyphylla sapling multiplication technical field, and in particular to a kind of fast-growing propagation method of the sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border.The inventive method includes:(1)The Paris polyphylla plant of the healthy no disease and pests harm of more than 4 years is planted in seed selection;(2)Cut off the aerial part of rhizome terminal bud and whole;(3)Rhizome tangent plane progress otch to staying in rhizome or exposed ground in soil;(4)Otch is carried out disinfection, dried;(5)Root dipping is carried out to the magnificent rhizoma paris rhizome with otch with GGR Freamine Ⅲs, while the sprayed boric fertilizer aqueous solution, dries, fertile soil is covered;(6)Field management is carried out to the magnificent rhizoma paris rhizome after covering fertile soil;(7)Many plants of healthy and strong sub- rhizome seedlings have been grown on Second Year autumn, the female rhizome of magnificent Paris polyphylla;(8)Win sub- rhizome seedling;(9)Sub- rhizome seedling is carried out disinfection, transplanted after drying;(10)Cultivation is harvested after 4 years.The present invention is emerged soon, and survival rate is high, is solved seedling in magnificent Paris polyphylla large-scale planting and is difficult the problem obtained, there is important productive value and application value.

Description

A kind of fast-growing mating system of the sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border
Technical field
The present invention relates to magnificent Paris polyphylla sapling multiplication technical field, and in particular to a kind of sub- root of magnificent Paris polyphylla for not destroying primary border The fast-growing propagation method of stem seedling.
Background technology
Magnificent Paris polyphyllaParis polyphylla Smith var.chinensis(Franch.) Hara. is western medicinal plant Industrialization is occupied in thing using one of leading position, most representative and global impact power resources of medicinal plant.But plant provides Source is seriously exhausted, produces magnificent Paris polyphylla secondary metabolites to alleviate the imbalance between supply and demand of Paris polyphylla resource still also not using cell engineering It can realize, tissue cultures breed magnificent Paris polyphylla seedling and do not obtain satisfactory result yet.In artificial cultivation proliferation, at present one The company of fixed number amount and the personal artificial growth for having started magnificent Paris polyphylla, have proceeded by magnificent Paris polyphylla cultivation property experiment.In recent years, permitted Many drugmakers establish magnificent Paris polyphylla and bred and planting base, have carried out large-scale introducing and planting.
But the present situation of magnificent Paris polyphylla industry cultivation is more chaotic, is mainly reflected in modes of reproduction:(1)Field excavation is directly planted Plant.Most area is all directly to plant the magnificent Paris polyphylla of field acquisition, and kind and germplasm mix, to the adaptability of miniclimate compared with Difference, causes the waste of introduces a collection, and survival rate is extremely low.(2)Rhizome Excision propagation.Medicinal material more than 2 rhizome sections is needed, can be consumed The medicinal material of commodity can be largely used as, and susceptible disease, breeding coefficient are low, one plant of magnificent Paris polyphylla with terminal bud Excision propagation is at most cut Cut a seedling stem.(3)Full rhizome cutting.The full rhizome cutting method bred without terminal bud, at most 6 rhizomes of cutting, rooting rate Also only have 40-50%, and most 3 plants survive, although breeding coefficient is more a little better than conventional rhizome segment method nursery, can not gram Take growth cycle length this problem.(4)Seminal propagation.Existing direct sowing has seedbed nursery again.But seed has hibernation feature, Seed then is directly sowed, and could sprout budding by 2 winters, and germination rate is less than 50%;Seedbed nursery, to working as Even if the seed of year knot takes the means of breaking dormancy to be bred, also it is only capable of shortening the germination cycle of 1 year.Seminal propagation Emergence rate is not high, and 8-15 is about needed from emerging to becoming a useful person, and cultivation management cost is higher.Four kinds of modess of reproduction of the above are produced Magnificent Paris polyphylla seedling, can not break its slow-growing, growth cycle length, breed the low biological characteristics of coefficient, seriously constrain Paris polyphylla resource regeneration and industrialized development.
Application No. " 201210364553.6 ", entitled " a kind of magnificent Paris polyphylla seedling is quick, the side efficiently bred Method ", this method includes the selection and processing of propagating materials;Sprouted using Stratificated treatment induction resting bud.Although the invention is improved Seedling speed and breeding coefficient, but this invention is the magnificent Paris polyphylla fast-growing breeding carried out in the case of the primary border of destruction, is planted Seedling survival rate can not reach desirable level, and complex operation.
Therefore, invent one kind and do not destroy the primary border of magnificent Paris polyphylla, and can realize method that China's Paris polyphylla seedling obtains rapidly very It is necessary.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of sub- rhizome of magnificent Paris polyphylla for not destroying primary border The fast-growing propagation method of seedling.
A kind of fast-growing propagation method for the sub- rhizome seedling of magnificent Paris polyphylla for not destroying primary border of the present invention, it includes following step Suddenly:
(1)9~December of First Year, the artificial growth of Paris polyphylla magnificent base, the health of selection plantation more than 4 years is without disease pest in flakes Harmful Paris polyphylla plant(Stem trace according to rhizome residue judges the Paris polyphylla plant age);
(2)The magnificent Paris polyphylla of underground is all embedded in for rhizome, from magnificent rhizoma paris rhizome head, with sterilization knife excision rhizome terminal bud and ground Upper plant part;It is remaining also with sterilization knife excision rhizome terminal bud and aerial part plant for the magnificent Paris polyphylla on partial denudation ground Rhizome be retained in underground all the time;
(3)Otch is carried out to the tangent plane for staying in the rhizome in soil with sterilization knife, otch wheel number is 1 wheel, otch number and neglected greatly Depending on the size of rhizome tangent plane, the fibrous root of magnificent rhizoma paris rhizome is not destroyed during otch as far as possible;The rhizome on partial denudation ground is carried out Otch, otch wheel number is 1~5 wheel, and distance is advisable with the internode distance of magnificent Paris polyphylla between often taking turns, and does not destroy Hua Chong during otch as far as possible The fibrous root of building rhizome;
(4)Otch is carried out disinfection, dried;
(5)Root dipping is carried out to the magnificent rhizoma paris rhizome with otch with GGR Freamine Ⅲs, while the sprayed boric fertilizer aqueous solution, squirt, Dried after spray thoroughly, cover fertile soil;
(6)Field management is carried out to the magnificent rhizoma paris rhizome after covering fertile soil;
(7)8 plants of healthy and strong sub- rhizome seedlings have at least been grown on Second Year autumn, each plant of magnificent female rhizome of Paris polyphylla;
(8)Second Year autumn or the 3rd year spring, plane open rhizome, win sub- rhizome seedling;
(9)Sub- rhizome seedling is carried out disinfection, transplanted after drying;
(10)After cultivation 4 years, commodity medicinal material is harvested, magnificent Paris polyphylla individual plant rhizome dry weight reaches more than 36.4g.
A kind of fast-growing propagation method of above-mentioned sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border, wherein step(4)Middle institute State otch plant ash or the sterilization of 75% Bravo wettable powder.
The fast-growing propagation method of above-mentioned a kind of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border, wherein the GGR amino Acid solutions are 50~200mg/kg, and the root dipping time is 5~10s.
The fast-growing propagation method of above-mentioned a kind of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border, wherein the boron fertilizer is water-soluble The mass percentage concentration of liquid is 1%~3%.
The fast-growing propagation method of above-mentioned a kind of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border, wherein the covering humic The thickness of soil is 2~4cm.
The fast-growing propagation method of above-mentioned a kind of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border, wherein the field management For:Using 72~76% shade rate, the timely watering pours water in arid, clean out a drain draining in time when rainwater is more.
A kind of fast-growing propagation method of above-mentioned sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border, wherein step(9)In adopt Sub- rhizome seedling is carried out disinfection with plant ash or 75% Bravo wettable powder.
The beneficial effects of the invention are as follows:
1st, carried out under primary border, do not destroy underground root system:Magnificent Paris polyphylla apical dominance removal work is carried out under primary border, will not be destroyed Magnificent Paris polyphylla underground root system and its ability to adapting to environment;Adaptation can be efficiently reduced relative to the propagation method for destroying primary border The time of environment, so as to improve survival rate;
2nd, according to the magnificent rhizoma paris rhizome own characteristic in primary border, operate respectively:Rhizome and partial denudation ground for being embedded in underground Rhizome, operated respectively.On this basis, magnificent Paris polyphylla cultivation base can be all converted to the sub- rhizome seedling of magnificent Paris polyphylla Breeding base;
3rd, the seedling breeding cycle is shortened:The method First Year is handled for the magnificent rhizoma paris rhizome in primary border, and Second Year just can be numerous Sub- rhizome seedling is brought out, 1~3 year soon breeding cycle of the seedling gone out than magnificent Paris polyphylla seminal propagation;
4th, improve and breed coefficient:Traditional rhizome cutting needs medicinal materials more than 2 rhizome sections, and one plant with terminal bud Excision propagation Magnificent Paris polyphylla at most one seedling stem of cutting;The method promotes lateral bud development to form sub- rhizome seedling by eliminating apical dominance, so Sub- rhizome seedling is also more, higher than traditional Propagation of Rhizomes coefficient more than 8 times;
5th, accelerate rate of development, shorten breeding cycle:Sub- rhizome seedling is cultivated 4 years, magnificent Paris polyphylla medicinal material total dry weight 36.4g with On, it is significantly higher than the weight of the magnificent Paris polyphylla medicinal materials of 8 years raw 23.1g of seminal propagation, shortens the magnificent Paris polyphylla fertility of more than 4 years week Phase, solve seedling in magnificent Paris polyphylla large-scale planting and be difficult the problem obtained, there is important productive value and application value.
Embodiment
Technical scheme is described in further detail with reference to specific embodiment, but protection scope of the present invention is not It is confined to as described below.
Embodiment 1
9~December of First Year, the healthy no disease and pests harm in the artificial growth of Paris polyphylla magnificent base, in flakes selection plantation more than 4 years Paris polyphylla plant(Stem trace according to rhizome residue judges the Paris polyphylla plant age).The Hua Chong of underground is all embedded in for rhizome Building, from magnificent rhizoma paris rhizome head, with sterilization knife excision rhizome terminal bud and aerial part plant;For the Hua Chong on partial denudation ground Building, also with sterilization knife excision rhizome terminal bud and aerial part plant, remaining rhizome is retained in underground all the time;To staying in soil The tangent plane of rhizome carries out otch, and otch wheel number is 1 wheel, otch number and depending on neglecting the size of rhizome tangent plane greatly;For part The aerial part of the rhizome of exposed ground, excision rhizome terminal bud and whole, otch wheel number is 3 wheels, and distance is with Hua Chong between often taking turns The internode distance in building is advisable.Otch is sterilized with plant ash or 75% Bravo wettable powder is sterilized.It is 60mg/kg's with concentration GGR Freamine Ⅲs carry out root dipping processing to the magnificent rhizoma paris rhizome with otch, and the root dipping time is 5s, while spraying quality percentage Concentration be 2% the boron fertilizer aqueous solution, squirt, spray thoroughly after dry, covering 2cm thickness fertile soil;Field management according to Paris polyphylla routine Technology:Timely watering pours water when 75% shade rate, arid, clean out a drain draining in time when rainwater is more;Second Year autumn, often Many plants of healthy and strong sub- rhizome seedlings can be grown on one plant of magnificent female rhizome of Paris polyphylla(Have bud, main root and radicula);The small rhizome won Seedling plant ash or the sterilization of 75% Bravo wettable powder, transplant after drying, after cultivating 4 years, can harvest magnificent Paris polyphylla medicinal material.
It is 9 plants, transplant survival that sub- rhizome seedling, which produces average strain number, on the female rhizome of the magnificent Paris polyphylla in each primary border of the present embodiment Rate is more than 95%, and after cultivating 4 years, magnificent Paris polyphylla individual plant rhizome dry weight reaches more than 36.4g.
Embodiment 2
9~December of First Year, the healthy no disease and pests harm in the artificial growth of Paris polyphylla magnificent base, in flakes selection plantation more than 4 years Paris polyphylla plant(Stem trace according to rhizome residue judges the Paris polyphylla plant age).The Hua Chong of underground is all embedded in for rhizome Building, from magnificent rhizoma paris rhizome head, with sterilization knife excision rhizome terminal bud and aerial part plant;For the Hua Chong on partial denudation ground Building, also with sterilization knife excision rhizome terminal bud and aerial part plant, remaining rhizome is retained in underground all the time;With sterilization knife to staying The tangent plane of rhizome in soil carries out otch, and otch wheel number is 1 wheel, otch number and depending on neglecting the size of rhizome tangent plane greatly; For the rhizome on partial denudation ground, the aerial part of excision rhizome terminal bud and whole, otch wheel number is 2 wheels, often the spacing of wheel From to be advisable with a distance from the internode of magnificent Paris polyphylla.Otch is sterilized with plant ash or 75% Bravo wettable powder is sterilized.It is with concentration 200mg/kg GGR Freamine Ⅲs carry out root dipping processing to the magnificent rhizoma paris rhizome with otch, and the root dipping time is 8s, is sprayed simultaneously Apply mass percentage concentration be 3% the boron fertilizer aqueous solution, squirt, spray thoroughly after dry, covering 4cm thickness fertile soil;Field management according to The conventional technique of Paris polyphylla:Timely watering pours water when 75% shade rate, arid, clean out a drain draining in time when rainwater is more;Second Many plants of healthy and strong sub- rhizome seedlings can be grown on autumn in year, each plant of magnificent female rhizome of Paris polyphylla(Have bud, main root and radicula);Win Small rhizome seedling plant ash or 75% Bravo wettable powder sterilization, after drying transplant, cultivation 4 years after, Hua Chong can be harvested Building medicinal material.
It is 13 plants, transplant survival that sub- rhizome seedling, which produces average strain number, on the female rhizome of the magnificent Paris polyphylla in each primary border of the present embodiment Rate is more than 95%, and after cultivating 4 years, magnificent Paris polyphylla individual plant rhizome dry weight reaches more than 38.3g.
Embodiment 3
9~December of First Year, the healthy no disease and pests harm in the artificial growth of Paris polyphylla magnificent base, in flakes selection plantation more than 4 years Paris polyphylla plant(Stem trace according to rhizome residue judges the Paris polyphylla plant age).The Hua Chong of underground is all embedded in for rhizome Building, from magnificent rhizoma paris rhizome head, with sterilization knife excision rhizome terminal bud and aerial part plant;For the Hua Chong on partial denudation ground Building, also with sterilization knife excision rhizome terminal bud and aerial part plant, remaining rhizome is retained in underground all the time;With sterilization knife to staying The tangent plane of rhizome in soil carries out otch, and otch wheel number is 1 wheel, otch number and depending on neglecting the size of rhizome tangent plane greatly; For the rhizome on partial denudation ground, the aerial part of excision rhizome terminal bud and whole, otch wheel number is 5 wheels, often the spacing of wheel From to be advisable with a distance from the internode of magnificent Paris polyphylla.Otch is sterilized with plant ash or 75% Bravo wettable powder is sterilized.It is with concentration 100mg/kg GGR Freamine Ⅲs carry out root dipping processing to the magnificent rhizoma paris rhizome with otch, and the root dipping time is 10s, simultaneously Spray mass percentage concentration be 1% the boron fertilizer aqueous solution, squirt, spray thoroughly after dry, covering 3cm thickness fertile soil;Field management is pressed According to the conventional technique of Paris polyphylla:Timely watering pours water when 80% shade rate, arid, clean out a drain draining in time when rainwater is more;The Many plants of healthy and strong sub- rhizome seedlings can be grown on 2 year autumn, each plant of magnificent female rhizome of Paris polyphylla(Have bud, main root and radicula);Pluck The small rhizome seedling plant ash taken or the sterilization of 75% Bravo wettable powder, transplant after drying, after cultivating 4 years, can harvest China Paris polyphylla medicinal material.
It is 10 plants, transplant survival that sub- rhizome seedling, which produces average strain number, on the female rhizome of the magnificent Paris polyphylla in each primary border of the present embodiment Rate is more than 95%, and after cultivating 4 years, magnificent Paris polyphylla individual plant rhizome dry weight reaches more than 37.7g.
Described above is only the preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein Form, is not to be taken as the exclusion to other embodiment, and available for various other combinations, modification and environment, and can be at this In the text contemplated scope, it is modified by the technology or knowledge of above-mentioned teaching or association area.And those skilled in the art are entered Capable change and change does not depart from the spirit and scope of the present invention, then all should appended claims of the present invention protection domain It is interior.

Claims (7)

1. a kind of fast-growing propagation method of the sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border, it is characterised in that including following step Suddenly:
(1)9~December of First Year, the artificial growth of Paris polyphylla magnificent base, the health of selection plantation more than 4 years is without disease pest in flakes Harmful Paris polyphylla plant;
(2)The magnificent Paris polyphylla of underground is all embedded in for rhizome, from magnificent rhizoma paris rhizome head, with sterilization knife excision rhizome terminal bud and ground Upper plant part;It is remaining also with sterilization knife excision rhizome terminal bud and aerial part plant for the magnificent Paris polyphylla on partial denudation ground Rhizome be retained in underground all the time;
(3)Otch is carried out in the tangent plane to staying in the rhizome in soil with sterilization knife, otch wheel number is 1 wheel;To partial denudation ground Rhizome carry out otch, otch wheel number be 1~5 wheel, often take turns between distance be advisable with the internode distance of magnificent Paris polyphylla, with not during otch The fibrous root of the magnificent rhizoma paris rhizome of destruction is target;
(4)Otch is carried out disinfection, dried;
(5)Root dipping is carried out to the magnificent rhizoma paris rhizome with otch with GGR Freamine Ⅲs, while the sprayed boric fertilizer aqueous solution, squirt, Dried after spray thoroughly, cover fertile soil;
(6)Field management to the magnificent rhizoma paris rhizome progress 1 year by a definite date after covering fertile soil;
(7)8 plants of healthy and strong sub- rhizome seedlings have at least been grown on Second Year autumn, each plant of magnificent female rhizome of Paris polyphylla;
(8)Second Year autumn or the 3rd year spring, plane issue female rhizome of sub- rhizome seedling, win sub- rhizome seedling;
(9)Sub- rhizome seedling is carried out disinfection, transplanted after drying;
(10)After cultivation 4 years, medicinal material is harvested, magnificent Paris polyphylla individual plant rhizome dry weight reaches more than 36.4g.
2. a kind of fast-growing propagation method of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border according to claim 1, its It is characterised by, step(4)Described in otch plant ash or 75% Bravo wettable powder sterilization.
3. a kind of fast-growing propagation method of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border according to claim 1 or 2, Characterized in that, the GGR Freamine Ⅲs concentration is 50~200mg/kg, the root dipping time is 5~10s.
4. a kind of fast-growing of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border according to any one of claims 1 to 3 is numerous Grow method, it is characterised in that the mass percentage concentration of the boron fertilizer aqueous solution is 1%~3%.
5. a kind of fast-growing of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border according to any one of Claims 1 to 4 is numerous Grow method, it is characterised in that the covering thickness humous is 2~4cm.
6. a kind of fast-growing of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border according to any one of Claims 1 to 5 is numerous Grow method, it is characterised in that the field management is:Using 70~80% shade rate, timely watering pours water, rainwater in arid Clean out a drain draining in time when many.
7. a kind of fast-growing of sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border according to any one of claim 1~6 is numerous Grow method, it is characterised in that step(9)Middle use plant ash or 75% Bravo wettable powder disappear to sub- rhizome seedling Poison.
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CN113994790A (en) * 2021-11-09 2022-02-01 广西壮族自治区中国科学院广西植物研究所 Method for promoting rapid germination and seedling emergence of paris polyphylla rhizome buds

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CN113994790A (en) * 2021-11-09 2022-02-01 广西壮族自治区中国科学院广西植物研究所 Method for promoting rapid germination and seedling emergence of paris polyphylla rhizome buds

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