CN103430838A - New technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation - Google Patents
New technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation Download PDFInfo
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- CN103430838A CN103430838A CN2013103149954A CN201310314995A CN103430838A CN 103430838 A CN103430838 A CN 103430838A CN 2013103149954 A CN2013103149954 A CN 2013103149954A CN 201310314995 A CN201310314995 A CN 201310314995A CN 103430838 A CN103430838 A CN 103430838A
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Abstract
The invention provides a new technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation. The new technology comprises: selecting a parispolyphylla rhizome to keep in reserve, drying in air for 3-7 days, making incisions on the back of the rhizome by using a cutter, immersing the incisions in an ABT rooting powder solution with a concentration of 10-30 ppm fro 2-10 h, disinfecting the incisions by plant ash, coating the parispolyphylla rhizome with a mixture of plant ash and clean river sands, transplanting and cultivating the parispolyphylla rhizome according to strains, coating fine soil with thickness of 5 mm on the parispolyphylla rhizome. After planting the rhizome, cluster buds germinate from stock plants and grow for about one year, then each parispolyphylla stock plant generates about more than twenty plantlets each of which possesses buds, small rhizome and a fine root, and the plantlets are taken as parispolyphylla seedlings for transplanting, and after 3-4 years, parispolyphylla finished products are obtained. The new technology has high propagation coefficient, and the produced parispolyphylla plantlets have high survival rate and fast growth speed. Compared with parispolyphylla seed propagation, the new technology helps to shorten more than one third of growth time of a finished product; and compared with a parispolyphylla rhizome fragment propagation method, the new technology helps to improve propagation coefficient by 4-6 times, and helps to solve the raw material gap facing medicinal materials and industrial production.
Description
Technical field
The cultivation that the present invention is medicinal plant, be specifically related to the efficient asexual multiplication seedling culture technique of magnificent Paris polyphylla.
Background technology
The rhizome that Chinese medicine China Paris polyphylla is Liliaceae Paris (Paris) perennial plant.2010 editions kinds of recording of the Pharmacopoeia of the People's Republic of China are magnificent Paris polyphylla Paris polyphylla Smith var.chinensis (Franch.) Hara and Yunnan China Paris polyphylla P.polyphylla Smith var.yunnanensis (Franch.) Han.-Mazz..China's Paris polyphylla bitter, cold in nature, slightly poisonous, there is the functions such as clearing heat and detoxicating, swelling and pain relieving, cool liver arresting convulsion.
Chinese medicine China Paris polyphylla contains the number of chemical composition, therefrom separates and has identified that more than 50 plant compound at present, mainly contains C
27steroid saponin, C
21pregnane glycosides, fatty acid ester, sterol and glycosides thereof, flavonoid glycoside, β-moulting hormone and polysaccharide etc.Wherein steroid saponin is 44 kinds, accounts for more than 80% of total compound, is the main active ingredient of magnificent Paris polyphylla treatment disease.A large amount of pharmacological researches show, the effects such as magnificent Paris polyphylla has hemostasis, suppresses tumour, eliminates the phlegm, antibacterial, analgesia, calmness and anti-cell poison.
China's Paris polyphylla is conventional Chinese medicine, it is also the primary raw material of the products such as famous Chinese patent drug Yunnan Baiyao, Gongxuening capsule, jidesheng sheyao tablets, antiviral granule, along with the research and development of new drug in recent years, the expansion of produce market, the demand cumulative year after year of Paris polyphylla raw material to China, wild resource far can not be met the need of market.Huge insufficiency of supply-demand drives the commodity price of medicinal material to skyrocket, and further stimulates medicinal herb grower's xcessive digging, and the Genuine producing area wild resource is seriously exhausted, has become in imminent danger, and it is imperative that the wild change man of magnificent Paris polyphylla plants.
Current magnificent Paris polyphylla wild product year of the whole nation, the amount of gathering was only 1700 tons of left and right, and demand side, take domestic large-scale herbal pharmaceutical factory of many families that the wide pharmaceutical factory in Sichuan and Yunnan Baiyao group be representative, China's Paris polyphylla annual requirement has reached more than 2000 tons, add the demand of hospital and pharmacy's formula, the market has openings of magnificent Paris polyphylla should be over 1500 tons.Market prospects and current resources situation in view of current magnificent Paris polyphylla, urgently carry out the artificial planting of scale China Paris polyphylla, growth characteristics due to magnificent Paris polyphylla plant, at present various places have all been carried out and have manually been introduced a fine variety wild instruction cultivation, but due to the fragmentary plantation that mostly is some areas, its breeding is a technical barrier, current seedling supply can not meet the tame requirement of magnificent Paris polyphylla far away, therefore be necessary Paris polyphylla biological nature to China fully understand with the basis of grasping on, adopt scientific and reasonable method to carry out the Fast-propagation research of magnificent Paris polyphylla, impel the raising reproduction coefficient, shorten the biological time limit, improve its quality, for the extensive growth of magnificent Paris polyphylla medicinal material improves effective approach.
Although in technology, also there is at present the segment of the rhizome of employing to carry out vegetative propagation and by methods such as seed seedling-raising breedings, there is the not high or poor growth of reproduction coefficient, be difficult to the problems such as large-scale promotion.
Summary of the invention
The object of the present invention is to provide a kind of employing rhizome otch, HORMONE TREATMENT, carry out the effective ways that magnificent Paris polyphylla seedling is produced in vegetative propagation.
The present invention contains following technical step:
(1), the perennial magnificent Paris polyphylla health of selecting field production is without the damage by disease and insect plant, its rhizome fresh weight is preferentially to select more than 80 grams, as breeding provenance, i.e. maternal plant;
(2), the magnificent rhizoma paris rhizome in 7-8 month using large Tanaka as the vegetative propagation provenance digs out, and uses the cutter after sterilization to prescind acrial part, rhizome is standby; Also can after gathering in the crops mature seed then, i.e. the 9-10 month, dig out underground rhizome, standby;
(3), after fresh rhizome digs out, the 3-7 days that dries in the air, with the cutter after sterilization, in rhizome back otch, incision length, depending on the rhizome size, generally should comprise 3-6 joint;
(4), after otch by magnificent rhizoma paris rhizome, especially cutting part is in the ABT of 10-30ppm rooting powder solution, or the Gibberellins solution immersion treatment 2-10 hour of 30-50ppm;
(5), with the ash magnificent rhizoma paris rhizome otch of sterilizing, take and be stained with otch as degree, then, after mixing with clean river sand with ash magnificent rhizoma paris rhizome being wrapped up in and mixed processing, press spacing in the rows 20cm, line-spacing 25cm transplants plantation, the above is coated with the fine earth of thick about 5mm;
(6), the plantation after, at once use the disinfecting soil sterilization processing of 50% carbendazol wettable powder 500-600 times liquid to the plant periphery;
(7), after rhizome plantation, under the conditioning of humidity and ground temperature, incision after treatment forms Multiple Buds, for improving incidence and the growth rate of Multiple Buds, should reach real-time weeding, applies fertilizer, scaffolding shades;
(8), Multiple Buds sprouts from maternal plant, after the growth of 1 year left and right time, each magnificent Paris polyphylla maternal plant can produce more than 20 strain plantlets, its bud, little rhizome, radicula all possess, plantlet length reaches 2-10cm, weight average reaches 2g; December, little rhizome is won from maternal plant, after processing with ash, transplanted as magnificent Paris polyphylla seedling; By above-mentioned step 1 to 7 carrying out (otch, the ash that disappears sterilization, wrap up in and mix then by spacing in the rows 20cm again, line-spacing 25cm transplants).
(9) gather in the crops magnificent Paris polyphylla finished product after 3-4.
Beneficial effect:
Under field conditions (factors), the rhizome of China's Paris polyphylla has resting bud because of its internode, work as truncated, when being subject to the wound such as insect pest or other factors and stimulating, resting bud can be sprouted, form one or more plantlets, but this kind of situation less generation under field conditions (factors) generally is no more than 10% through observing incidence.Technology of the present invention can promote the generation of plantlet greatly, for the breeding of magnificent Paris polyphylla, growth provide favourable means.
The inventive method reproduction coefficient is high, and the magnificent Paris polyphylla plantlet survival rate of producing is high, fast growth.Compare with magnificent Paris polyphylla seminal propagation, can shorten the finished product growth time and reach more than 1/3rd, can greatly improve the costs such as soil, labour, management, improved the unit are economic benefit; Compare with magnificent rhizoma paris rhizome cutting section propagation method, can improve reproduction coefficient 4-6 doubly, in the situation that scarcity of resources, seedling are rare at present, can greatly accelerate the industrialization speed of magnificent Paris polyphylla, solve the breach that medicinal material and industrial production raw material face.
Annual vegetative plantlet rhizome can reach the size through 3 years stems of taking root of seminal propagation, and plantlet shows obvious fast-growing after transplanting, and 3-4 can gather in the crops finished product, can make the Paris polyphylla product shorten 1/3rd vegetative period.
Adopt the present invention, compare with rhizoma paris rhizome segment method, reproduction coefficient can improve greatly, general each Paris polyphylla maternal plant can produce more than 20 strain plantlets, and segment method the first Paris polyphylla maternal plant generally can be cut into the 3-5 section, form 3-5 plant, this law reproduction coefficient is 4-6 times of conventional segment method.
Embodiment
Embodiment 1
Select magnificent Paris polyphylla healthy plant, in the 7-8 month, dig out rhizome, use the cutter after sterilization to prescind acrial part, in rhizome back otch, otch generally should comprise 3-6 joint.The ABT rooting powder solution immersion treatment rhizome of employing 30ppm 4 hours.With the ash otch of sterilizing, then mixes with clean river sand with ash and wrap up in the rhizome of mixing after sterile-processed, press seeding row spacing 20x25 transplanting, adopt 500 times of liquid of 50% carbendazol wettable powder to process soil disinfection.Induce the formation Multiple Buds, and and then cultivate into plantlet, win and can be used as the transplanting seedling.
On each magnificent Paris polyphylla maternal plant of the present embodiment, plantlet produces 23 strains of number average out to, and transplanting survival rate is more than 9596.
Embodiment 2
Select magnificent Paris polyphylla healthy plant, after gathering in the crops seed in the 9-10 month, dig out rhizome, use through the sterilization after cutter in rhizome back otch, otch should comprise 3-6 joint.The Gibberellins solution immersion treatment rhizome of employing 50ppm 8 hours.With the ash otch of sterilizing, then mixes to wrap up in clean river sand with ash and mix the rhizome of processing after sterilization, press seeding row spacing 20x25 transplanting.Adopt 600 times of liquid of 50% carbendazol wettable powder to process planting disinfecting soil.Induced bundle is sprouted, and cultivates into plantlet, wins plantlet as transplanting seedling.
On each magnificent Paris polyphylla maternal plant of the present embodiment, plantlet produces 18 strains of number average out to, and transplanting survival rate is more than 95%.
Foregoing description is exemplary description.
Claims (1)
1. from the sprout new technology of fast asexual propagation of magnificent rhizoma paris rhizome induced bundle, it is characterized in that, contain following technical step:
(1), the perennial magnificent Paris polyphylla health of selecting field production is without the damage by disease and insect plant, its rhizome fresh weight is preferentially to select more than 80 grams, as breeding provenance, i.e. maternal plant;
(2), the magnificent rhizoma paris rhizome in 7-8 month using large Tanaka as the vegetative propagation provenance digs out, and uses the cutter after sterilization to prescind acrial part, rhizome is standby; Also can after gathering in the crops mature seed then, i.e. the 9-10 month, dig out underground rhizome, standby;
(3), after fresh rhizome digs out, the 3-7 days that dries in the air, with the cutter after sterilization, in rhizome back otch, incision length, depending on the rhizome size, generally should comprise 3-6 joint;
(4), after otch by magnificent rhizoma paris rhizome, especially cutting part is in the ABT of 10-30ppm rooting powder solution, or the Gibberellins solution immersion treatment 2-10 hour of 30-50ppm;
(5), with the ash magnificent rhizoma paris rhizome otch of sterilizing, take and be stained with otch as degree, then, after mixing with clean river sand with ash magnificent rhizoma paris rhizome being wrapped up in and mixed processing, press spacing in the rows 20cm, line-spacing 25cm transplants plantation, the above is coated with the fine earth of thick about 5mm;
(6), the plantation after, at once use the disinfecting soil sterilization processing of 50% carbendazol wettable powder 500-600 times liquid to the plant periphery;
(7), after rhizome plantation, under the conditioning of humidity and ground temperature, incision after treatment forms Multiple Buds, for improving incidence and the growth rate of Multiple Buds, should reach real-time weeding, applies fertilizer, scaffolding shades;
(8), Multiple Buds sprouts from maternal plant, after the growth of 1 year left and right time, each magnificent Paris polyphylla maternal plant can produce more than 20 strain plantlets, its bud, little rhizome, radicula all possess, plantlet length reaches 2-10cm, weight average reaches 2g; December, little rhizome is won from maternal plant, after processing with ash, transplanted as magnificent Paris polyphylla seedling; Its transplanting method is undertaken by above-mentioned steps 1 to 7;
(9) gather in the crops magnificent Paris polyphylla finished product after 3-4.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104718842A (en) * | 2015-03-17 | 2015-06-24 | 铜仁职业技术学院 | Paris polyphylla husking seedling-emerging method |
| CN104904474A (en) * | 2015-06-07 | 2015-09-16 | 湖北济世药业有限责任公司 | Paris polyphylla asexual reproduction method |
| CN106069106A (en) * | 2016-06-30 | 2016-11-09 | 上海市园林科学规划研究院 | A kind of Afriocan agapanthus promotees the propagation method of bud plant division |
| CN106212038A (en) * | 2016-09-29 | 2016-12-14 | 紫云自治县紫香源农林科技有限责任公司 | A kind of rhizoma paris rhizome propagation method |
| CN107278579A (en) * | 2017-07-20 | 2017-10-24 | 四川省中医药科学院 | A kind of fast-growing mating system of the sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border |
| CN109349019A (en) * | 2018-12-10 | 2019-02-19 | 怀化正好制药有限公司 | The cultural method of chinaroot greenbrier |
| CN109479722A (en) * | 2019-01-03 | 2019-03-19 | 陕西中医药大学 | A kind of new method of artificial induction's Paris polyphylla pierre |
| CN111448963A (en) * | 2020-05-14 | 2020-07-28 | 盐津中港中药材专业合作社 | Quick-growing method for cutting root and keeping plant of Paris polyphylla |
| CN113994790A (en) * | 2021-11-09 | 2022-02-01 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting rapid germination and seedling emergence of paris polyphylla rhizome buds |
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| CN1602656A (en) * | 2004-11-01 | 2005-04-06 | 成都中康光大药业有限公司 | Wild Paris chinensis Franch artificial domestication grow seedling and cultivation method |
| CN102870564A (en) * | 2012-09-26 | 2013-01-16 | 四川农业大学 | Fast efficient propagation method of Paris polyphylla |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104718842A (en) * | 2015-03-17 | 2015-06-24 | 铜仁职业技术学院 | Paris polyphylla husking seedling-emerging method |
| CN104904474A (en) * | 2015-06-07 | 2015-09-16 | 湖北济世药业有限责任公司 | Paris polyphylla asexual reproduction method |
| CN104904474B (en) * | 2015-06-07 | 2017-06-30 | 湖北济世药业有限责任公司 | A kind of asexual reproduction method of Paris polyphylla |
| CN106069106A (en) * | 2016-06-30 | 2016-11-09 | 上海市园林科学规划研究院 | A kind of Afriocan agapanthus promotees the propagation method of bud plant division |
| CN106212038A (en) * | 2016-09-29 | 2016-12-14 | 紫云自治县紫香源农林科技有限责任公司 | A kind of rhizoma paris rhizome propagation method |
| CN107278579A (en) * | 2017-07-20 | 2017-10-24 | 四川省中医药科学院 | A kind of fast-growing mating system of the sub- rhizome seedling of the magnificent Paris polyphylla for not destroying primary border |
| CN107278579B (en) * | 2017-07-20 | 2020-04-07 | 四川省中医药科学院 | Fast-growing breeding method of Paris polyphylla rhizome seedlings without destroying original habitat |
| CN109349019A (en) * | 2018-12-10 | 2019-02-19 | 怀化正好制药有限公司 | The cultural method of chinaroot greenbrier |
| CN109479722A (en) * | 2019-01-03 | 2019-03-19 | 陕西中医药大学 | A kind of new method of artificial induction's Paris polyphylla pierre |
| CN111448963A (en) * | 2020-05-14 | 2020-07-28 | 盐津中港中药材专业合作社 | Quick-growing method for cutting root and keeping plant of Paris polyphylla |
| CN113994790A (en) * | 2021-11-09 | 2022-02-01 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting rapid germination and seedling emergence of paris polyphylla rhizome buds |
| CN113994790B (en) * | 2021-11-09 | 2022-07-22 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting quick germination and seedling emergence of paris polyphylla rhizome buds |
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Application publication date: 20131211 |