CN104770103A - Seedling raising method for oil-used peony seeds - Google Patents

Abstract

The invention discloses a seedling raising method for oil-used peony seeds. The method comprises the steps of (1) selecting seeds; (2) soaking the seeds for disinfecting; (3) treating the oil-used peony seeds of step (2) by using cold plasma generated by atmospheric dielectric barrier discharge for 50-60 seconds; (4) preparing a treatment solution containing 0.25mg/L of gibberellin, 2mg/L of indoleacetic acid and 3mg/L of calcium chloride; (5) performing variable temperature stratification: mixing the oil-used peony seeds treated in step (3) with fine sand, perlite and vermiculite in a ratio of 1:(3-5):(1-2):(1-2), adjusting the humidity to 40-50%, and performing variable temperature stratification in a constant temperature incubator according to three stages; (6) sowing; and (7) performing seedling management. By adopting the seedling raising method for the oil-used peony seeds, seed dormancy can be effectively broken during breeding, so that seeds are quickly germinated, germination of seeds is accelerated, the emergence rate of seeds is improved, the emergence time is shortened, the emergence is even, and the growth indexes of young seedlings such as fresh weight, plant height, root system and the like are improved to different degrees; and the method does not have pollution to the environment.

Description

The method of a kind of oil Paeonia suffruticosa seed nursery

Technical field

The present invention relates to flower seed seedling growing process field, particularly relate to the method for a kind of oil Paeonia suffruticosa seed nursery.

Background technology

Oil is the one of national flower tree peony with tree peony, it is the plant that traditional oil tree has again medical value, also be the high green tree species of ornamental value, strong adaptability, drought-resistant barren, not only be adapted at hilly country but also be adapted at the growth of flat area, the north of the Changjiang River, Anhui, be implement soil synergy, afforest rural area, improve the fine tree species of ecological condition.Oil its root skin of tree peony has analgesia, refrigerant antipyretic, anti-inflammation, immunity etc. medicinal, and element and the root of herbaceous peony, chrysanthemum, Poria cocos be called Anhui four famous medicine greatly are also one of national 34 kinds of rare medicinal herbss.Peony seed oil has certain curative effect for common cardiovascular diseases such as prevention and therapy hypertension, highs fat of blood, also has significant food therapy effect to diabetic, and without any bad reaction.Current oil often has Ye Wuhua aborning with tree peony or has Hua Wuye, the phenomenon even do not sprouted, this mainly because oil with tree peony bud not by breaking dormancy or termination of diapause is insufficient causes, oil has the characteristic of top-wall effect with tree peony, after autumn sowing, radicle growth becomes root and epicotyl does not extend, and is in resting state, and after having to pass through the low temperature of a period of time, epicotyl could be sprouted.In addition, current oil is low with tree peony germination rate, and most seed is being sowed for a long time and losing vigor in storage, and loss of seeds is serious.

Summary of the invention

The object of the invention is to overcome the deficiencies in the prior art, the method of a kind of oil Paeonia suffruticosa seed nursery is provided, in cultivating process, effectively can abolish seed dormancy, seed is germinateed rapidly, accelerate seed germination, improve seeding ratio, shorten seedling-growing time, emerge neat, and improve the growth indexes such as fresh weight, plant height, root system of seedling to some extent, environmentally safe.

The present invention adopts following technical scheme to achieve these goals:

A method for oil Paeonia suffruticosa seed nursery, comprises the following steps:

(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, remove impurity and flat grain by clear water floatation, choose healthy oil Paeonia suffruticosa seed, oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%;

(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours, take out again and be filtered dry the surface of the seed moisture, then the hypochlorite disinfectant 10-15 minute of 10% is used, aseptic water washing 3-5 time, for subsequent use after the surface of the seed moisture that dries in the shade;

(3) the oil Paeonia suffruticosa seed of the cold plasma treatment step (2) utilizing atmospheric dielectric barrier discharge to generate, the time of plasma treatment seed is 50-60s;

(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L;

(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) being processed mixes in the ratio of 1:3-5:1-2:1-2 with fine sand, perlite, vermiculite, adjust humidity to 40-50%, according to the alternating temperature stratification process carrying out three phases as follows in constant incubator:

In the stage one: lamination 5-8 days under 10-20 DEG C of condition, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage two: lamination 15-20 days under 3-6 DEG C of condition, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage three: lamination 5-8 days under-2-3 DEG C of condition, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once, obtains the seed sprouted;

(6) sow: adopt drilling method to be sown into by the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order, broadcast rear covering thin soil and hide seed, then cover with withered grass, cultivate seed sprouting;

(7) seedling management: topdress in seedling stage, when oil exposes perula with tree peony flower bud development to leaflet petiole, peels off outside scale and bottom 2-3 sheet leaflet.

The power supply of the dielectric barrier discharge described in step (3) is high-frequency and high-voltage power supply, and frequency is 3-5kHz, and voltage is 5-7kV.

Compared with the prior art, beneficial effect of the present invention is as follows:

(1) method of oil Paeonia suffruticosa seed of the present invention nursery, in cultivating process, technology such as comprehensive utilization growth regulator and plasma treatment etc. breaks seed dormancy, seed is germinateed rapidly, accelerates seed germination, improve seeding ratio, shorten seedling-growing time, emerge neat, and improve the growth indexes such as fresh weight, plant height, root system of seedling to some extent, environmentally safe;

(2) the present invention ensure that seed quality and vigor, water seed soaking by the seed selection of application clear water floatation, makes the abundant imbibition of seed; Hypochlorite disinfectant, prevents seed from going mouldy; Comprehensive utilization growth regulator and plasma treatment, relieve the dormancy of oil Paeonia suffruticosa seed effectively; Utilize the performance of gibberellin, break the seed dormancy phase, impel and germinate ahead of time, and improve germination rate; Utilize the performance of heteroauxin, promote oil tree peony kind seedling rooting, improve the output of moutan root bark.

Embodiment

Below in conjunction with embodiment, the present invention is further illustrated, but the present invention is not limited only to these embodiments, and under the prerequisite not departing from present inventive concept, any improvement done all drops within protection scope of the present invention.

Embodiment 1:

A method for oil Paeonia suffruticosa seed nursery, comprises the following steps:

(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, remove impurity and flat grain by clear water floatation, choose healthy oil Paeonia suffruticosa seed, oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%;

(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours, take out again and be filtered dry the surface of the seed moisture, then the hypochlorite disinfectant 10 minutes of 10% is used, aseptic water washing 5 times, for subsequent use after the surface of the seed moisture that dries in the shade;

(3) the oil Paeonia suffruticosa seed of the cold plasma treatment step (2) utilizing atmospheric dielectric barrier discharge to generate, the time of plasma treatment seed is 50s; The power supply of described dielectric barrier discharge is high-frequency and high-voltage power supply, and frequency is 3kHz, and voltage is 5kV.

(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L;

(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) being processed mixes in the ratio of 1:3:2:2 with fine sand, perlite, vermiculite, adjustment humidity to 40%, according to the alternating temperature stratification process carrying out three phases as follows in constant incubator:

In the stage one: lamination 8 days under 10 DEG C of conditions, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage two: lamination 20 days under 3 DEG C of conditions, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage three: lamination 8 days under-2 DEG C of conditions, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once, obtains the seed sprouted;

(6) sow: adopt drilling method to be sown into by the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order, broadcast rear covering thin soil and hide seed, then cover with withered grass, cultivate seed sprouting;

(7) seedling management: topdress in seedling stage, when oil exposes perula with tree peony flower bud development to leaflet petiole, peels off outside scale and 2, bottom leaflet.

Embodiment 2:

A method for oil Paeonia suffruticosa seed nursery, comprises the following steps:

(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, remove impurity and flat grain by clear water floatation, choose healthy oil Paeonia suffruticosa seed, oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%;

(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours, take out again and be filtered dry the surface of the seed moisture, then the hypochlorite disinfectant 15 minutes of 10% is used, aseptic water washing 3-5 time, for subsequent use after the surface of the seed moisture that dries in the shade;

(3) the oil Paeonia suffruticosa seed of the cold plasma treatment step (2) utilizing atmospheric dielectric barrier discharge to generate, the time of plasma treatment seed is 60s; The power supply of described dielectric barrier discharge is high-frequency and high-voltage power supply, and frequency is 5kHz, and voltage is 7kV.

(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L;

(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) being processed mixes in the ratio of 1:5:1:1 with fine sand, perlite, vermiculite, adjustment humidity to 50%, according to the alternating temperature stratification process carrying out three phases as follows in constant incubator:

In the stage one: lamination 5 days under 20 DEG C of conditions, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage two: lamination 15 days under 6 DEG C of conditions, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage three: lamination 5 days under-3 DEG C of conditions, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once, obtains the seed sprouted;

(6) sow: adopt drilling method to be sown into by the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order, broadcast rear covering thin soil and hide seed, then cover with withered grass, cultivate seed sprouting;

(7) seedling management: topdress in seedling stage, when oil exposes perula with tree peony flower bud development to leaflet petiole, peels off outside scale and 3, bottom leaflet.

Claims (2)

1. an oil method for Paeonia suffruticosa seed nursery, is characterized in that: comprise the following steps:

(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, remove impurity and flat grain by clear water floatation, choose healthy oil Paeonia suffruticosa seed, oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%;

(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours, take out again and be filtered dry the surface of the seed moisture, then the hypochlorite disinfectant 10-15 minute of 10% is used, aseptic water washing 3-5 time, for subsequent use after the surface of the seed moisture that dries in the shade;

(3) the oil Paeonia suffruticosa seed of the cold plasma treatment step (2) utilizing atmospheric dielectric barrier discharge to generate, the time of plasma treatment seed is 50-60s;

(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L;

(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) being processed mixes in the ratio of 1:3-5:1-2:1-2 with fine sand, perlite, vermiculite, adjust humidity to 40-50%, according to the alternating temperature stratification process carrying out three phases as follows in constant incubator:

In the stage one: lamination 5-8 days under 10-20 DEG C of condition, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage two: lamination 15-20 days under 3-6 DEG C of condition, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once;

In the stage three: lamination 5-8 days under-2-3 DEG C of condition, the treatment fluid that step (4) is prepared constantly is stirred and sprayed to period, is limited with Paeonia suffruticosa seed and fine sand, perlite, vermiculite, and spray every day once, obtains the seed sprouted;

(6) sow: adopt drilling method to be sown into by the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order, broadcast rear covering thin soil and hide seed, then cover with withered grass, cultivate seed sprouting;

(7) seedling management: topdress in seedling stage, when oil exposes perula with tree peony flower bud development to leaflet petiole, peels off outside scale and bottom 2-3 sheet leaflet.

2. the method for oil Paeonia suffruticosa seed according to claim 1 nursery, is characterized in that: the power supply of the dielectric barrier discharge described in step (3) is high-frequency and high-voltage power supply, and frequency is 3-5kHz, and voltage is 5-7kV.