CN106821938A - 一种人间充质干细胞冻干粉的制备方法 - Google Patents
一种人间充质干细胞冻干粉的制备方法 Download PDFInfo
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- 230000002103 transcriptional effect Effects 0.000 description 1
- 210000003606 umbilical vein Anatomy 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
- 210000004509 vascular smooth muscle cell Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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Classifications
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
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- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
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- A61K8/817—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen; Compositions or derivatives of such polymers, e.g. vinylimidazol, vinylcaprolactame, allylamines (Polyquaternium 6)
- A61K8/8176—Homopolymers of N-vinyl-pyrrolidones. Compositions of derivatives of such polymers
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- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
Description
序号 | 活性成分 | 含量(%) |
1 | 海藻糖 | 0.1g/100ml |
2 | 右旋糖酐 | 0.1g/100ml |
3 | 多聚精氨酸 | 0.1mg/100ml |
4 | 甘露醇 | 0.1g/100ml |
5 | 羟乙基淀粉 | 0.1g/100ml |
6 | 泊洛沙姆(P188) | 0.1g/100ml |
7 | 丙三醇 | 0.1g/100ml |
8 | 蔗糖 | 0.1g/100ml(0.25mol/L) |
9 | 聚乙烯吡咯烷酮 | 0.1g/100ml |
序号 | 活性成分 | 含量(%) |
1 | 海藻糖 | 9g/100ml |
2 | 右旋糖酐 | 15g/100ml |
3 | 多聚精氨酸 | 5mg/100ml |
4 | 甘露醇 | 5g/100ml |
5 | 羟乙基淀粉 | 5g/100ml |
6 | 泊洛沙姆(P188) | 3g/100ml |
7 | 丙三醇 | 4g/100ml |
8 | 蔗糖 | 5g/100ml(0.25mol/L) |
9 | 聚乙烯吡咯烷酮 | 5g/100ml |
Claims (6)
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Cited By (16)
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CN107744526A (zh) * | 2017-10-30 | 2018-03-02 | 南京医科大学 | 羊干素及其制备方法和应用 |
CN108309822A (zh) * | 2018-02-26 | 2018-07-24 | 福建省银丰干细胞工程有限公司 | 一种人脐带间充质干细胞旁分泌因子冻干粉的制备方法 |
CN108782544A (zh) * | 2018-06-28 | 2018-11-13 | 吉林省拓华生物科技有限公司 | 一种大量冻存间充质干细胞的冻存液及方法 |
CN109287622A (zh) * | 2018-11-27 | 2019-02-01 | 南京三生生物技术股份有限公司 | 一种骨髓回输治疗伴hiv感染的结直肠癌和失代偿肝硬化合并小肝癌用的组织保存液 |
CN110317781A (zh) * | 2019-07-18 | 2019-10-11 | 天津博雅秀岩生物技术有限公司 | 间充质干细胞冻存和复苏的方法 |
CN110343245A (zh) * | 2019-07-16 | 2019-10-18 | 天津大学 | 一种ε-聚赖氨酸-接枝-疏水氨基酸-接枝-海藻糖的糖肽及制备方法 |
CN110946129A (zh) * | 2019-11-08 | 2020-04-03 | 浙江卫未生物医药科技有限公司 | 细胞复苏后高活率冻存液 |
CN111351930A (zh) * | 2020-02-20 | 2020-06-30 | 泛肽生物科技(浙江)有限公司 | 一种活性人淋巴细胞表面抗原质控品及其制备方法 |
CN111690601A (zh) * | 2020-06-08 | 2020-09-22 | 海南优尼科尔生物科技有限公司 | 一种脐带间充质干细胞制剂的制备方法 |
CN111869659A (zh) * | 2020-08-24 | 2020-11-03 | 上海雅酶生物医药科技有限公司 | 一种无血清细胞冻存液、制备方法及其使用方法 |
WO2020258156A1 (en) * | 2019-06-27 | 2020-12-30 | Nanjing Mucyte Biotechnology Co., Ltd | Cell preservation and preparative medium and method for using the same |
CN113367123A (zh) * | 2021-06-07 | 2021-09-10 | 南京三生生物技术股份有限公司 | 一种细胞冻存方法 |
CN114007416A (zh) * | 2019-04-30 | 2022-02-01 | 明尼苏达大学董事会 | 用于细胞的冷冻保存的组合物和方法 |
CN114015758A (zh) * | 2021-10-15 | 2022-02-08 | 无锡百泰克生物技术有限公司 | 一种冻干保护剂、荧光pcr检测试剂盒及冻干工艺 |
CN114540289A (zh) * | 2022-02-15 | 2022-05-27 | 杭州清大科瑞生物科技有限公司 | 一种间充质干细胞培养液冻干工艺 |
CN116889228A (zh) * | 2023-07-12 | 2023-10-17 | 重庆市铂而斐细胞生物技术有限公司 | 一种脐带间充质干细胞的冻存方法 |
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CN107744526A (zh) * | 2017-10-30 | 2018-03-02 | 南京医科大学 | 羊干素及其制备方法和应用 |
CN108309822A (zh) * | 2018-02-26 | 2018-07-24 | 福建省银丰干细胞工程有限公司 | 一种人脐带间充质干细胞旁分泌因子冻干粉的制备方法 |
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CN113367123B (zh) * | 2021-06-07 | 2022-05-13 | 南京三生生物技术股份有限公司 | 一种细胞冻存方法 |
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CN114540289A (zh) * | 2022-02-15 | 2022-05-27 | 杭州清大科瑞生物科技有限公司 | 一种间充质干细胞培养液冻干工艺 |
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