CN106561210B - Utilize the method for potato stem section numerous micro potato fastly - Google Patents
Utilize the method for potato stem section numerous micro potato fastly Download PDFInfo
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- CN106561210B CN106561210B CN201610917784.3A CN201610917784A CN106561210B CN 106561210 B CN106561210 B CN 106561210B CN 201610917784 A CN201610917784 A CN 201610917784A CN 106561210 B CN106561210 B CN 106561210B
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- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 99
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 84
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000011159 matrix material Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 150000001875 compounds Chemical class 0.000 claims abstract description 5
- 241000238631 Hexapoda Species 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 8
- 239000010451 perlite Substances 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 3
- 238000002054 transplantation Methods 0.000 claims description 3
- 230000000422 nocturnal effect Effects 0.000 claims 1
- 238000003306 harvesting Methods 0.000 abstract description 9
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 1
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical group OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of methods using potato stem section numerous micro potato fastly, successively the following steps are included: preparing cultivation matrix；Cultivation matrix is dispensed into seedling-growing container, after being irrigated with water, transplants the potato set of a height of 7~8cm；It is then placed in culture in the net canopy equipped with sunshade net and insect preventing net cover；Potato plant wait cultivate it is long to 28~32cm when, top is cut away, and potato plant stem section is then cut into the stem section with a piece of compound leaf；Cultivation matrix is dispensed into hole tray, is irrigated with water；Above-mentioned potato plant stem section is cultivated by the cuttage upward of morphology upper end into hole tray, when cuttage covers petiole base just by cultivation matrix；After bearing the micro potato of 0.8~1.2g weight at the leaf segment of the potato stem section of cuttage, and this stem section is extracted, and harvest micro potato.
Description
Technical field
The present invention relates to a kind of fast breeding techniques of micro potato, specifically numerous fastly using potato haulm segment cuttage technology
Micro potato.
Background technique
Potato (Solanum tuberosum) is a kind of widely distributed, full of nutrition grain dish dual-purpose type crop, at me
State is the fourth-largest crop for being only second to wheat, rice and corn.
Currently, mainly breeding potato breeding and toxicity-removing white potato using plant tissue culture technique, which is needed
To achieve the purpose that fast numerous micro potato by squamous subculture.Squamous subculture breeding coefficient is high, can rapidly obtain big
Batch seedling, but there are some disadvantages by tissue cultures expansion breeding seedling: first, equipment requirement is high, it is desirable that has profession
Tissue culture laboratory；Second, tissue culture is higher to technical operation level requirement, is easy pollution；Third, agar powder valence used in tissue culture
Lattice are higher, high production cost.
Another method is to achieve the purpose that fast numerous potato seed by vitro axil bud cutting technology.Wang Mengfei et al. is
(patent publication No. CN102057834A) is applied for a patent, the technology is the following steps are included: maternal plant cultivation, axillary bud deriving, cutting bed
Preparation, axillary bud shearing, the processing of in vitro axillary bud, temperature regulation and management, this method induced production and use by cultivation in 4~5 weeks
Micro potato.This method is at low cost, and breeding coefficient is high, but the technology has the following deficiencies: first, needs using N- bis-
Methylamino succinyl acid and KH2PO4To induce axillary bud.Second, in vitro axillary bud just can be carried out skewer after needing to handle using exogenous hormone
It inserts, operation is more complicated, time-consuming and laborious.
Summary of the invention
Potato stem section numerous micro potato (miniature horse fastly is utilized the technical problem to be solved in the present invention is to provide a kind of
Bell potato) method, this method is easy to operate, and production cost is low, and can rapidly expand numerous micro potato.
In order to solve the above technical problem, the present invention provides a kind of utilization potato stem section, numerous micro potato is (micro- fastly
Type potato) method, successively the following steps are included:
1), the preparation of cultivation matrix:
Turf, vermiculite, perlite are mixed by the mass ratio of 1:0.9~1.1:0.9~1.1, are configured to cultivation matrix；
2), test tube transplantation of seedlings:
The resulting cultivation matrix of step 1) is dispensed into seedling-growing container, after being irrigated with water, transplants the Ma Ling of a height of 7~8cm
Potato seedling growth；It is then placed in culture in the net canopy equipped with sunshade net and insect preventing net cover, waters in due course in incubation, controls daytime (i.e. 6
Point~18 point) temperature be 28~30 DEG C, night (i.e. 18 points~6 points) temperature be 18~20 DEG C, relative humidity be 70%~90%；
Remarks explanation: above-mentioned in due course watering is carries out according to routine techniques, that is, when water content≤12%, watering until
Water content is 18%, and the % is quality %；
Insect preventing net cover is, for example, 40 mesh, and the purpose is to pest controls；The fly net covers seedling-growing container；
3), stem section is sheared:
When the potato plant of step 2) culture it is long to 28~32cm (preferably 30cm or so) when, cut away and (cut with blade
Fall) 4~6cm of plant top (preferably 5cm), potato plant stem section is then cut into the stem section with a piece of compound leaf, stem section
Upper and lower notch is 1 ± 0.1cm away from petiole base；
4), stem section cuttage:
The resulting cultivation matrix of step 1) is dispensed into hole tray, is irrigated with water；
Step 3) potato plant stem section obtained is cultivated by the cuttage upward of morphology upper end into hole tray, skewer
Petiole base is covered just by cultivation matrix when slotting；The same step 2 of condition of culture；
Remarks illustrate: may determine that morphology upper end, the end of blade upward are morphology according to the blade direction in stem section
Upper end；
5) micro potato, is harvested:
Micro potato (the skewer of 0.8~1.2g weight (that is, about 1g) is born at the leaf segment of the potato stem section of cuttage in step 4)
Slotting incubation time about 18~22 days) after, and this stem section is extracted, and harvest micro potato.
As the improvement of the invention using the potato stem section method of numerous micro potato fastly: in the step 1),
Turf: vermiculite: perlite=1:1:1 mass ratio.
Using method of the invention, it can be proliferated within 4~5 weeks or so once, can once be proliferated 5~10 times.Cuttage obtains
Micro potato save, transport it is all very convenient, be especially advantageous for the popularization and application of the technology.In addition it can the micro potato that will be harvested
Continue to plant seedling, achievees the purpose that further to be proliferated by way of cuttage repeatedly.
Stem section mine needed for each period during numerous micro potato fastly is able to satisfy using cultivation matrix of the invention
Prime element does not need in addition to apply fertilizer.
Stem section with a piece of compound leaf is directly carried out cuttage by the present invention, and not needing to be handled using exogenous hormone can
Successfully obtain micro potato.
In conclusion it is fast numerous with easy to operate, low in cost, reproduction speed to carry out micro potato using the present invention
Fastly, the features such as breeding coefficient is high.Therefore, the present invention is highly suitable for the fast numerous of micro potato.
Detailed description of the invention
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing.
Fig. 1 is the micro potato schematic diagram born at the potato stem section leaf segment of cuttage；
Fig. 2 is the micro potato schematic diagram harvested from the potato stem section of cuttage；
Fig. 3 is the seedling effect picture that the micro potato of harvest grows up to.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This.
Embodiment 1: potato haulm segment cuttage technology numerous color potato powder system kind ' cherie ' micro potato fastly is utilized
Method successively follows the steps below:
1), the preparation of cultivation matrix:
Turf, vermiculite, perlite are mixed by the mass ratio of 1:1:1, are configured to cultivation matrix；
2), test tube transplantation of seedlings:
At the beginning of 4 months, cultivation matrix is dispensed into seedling-growing container, is irrigated with water.From tissue culture room take growing way preferably, plant height be 7~
The virus-free potato tube plant of 8cm, seedling-growing container is placed in the net canopy for being stamped sunshade net by plantation into seedling-growing container after practicing seedling
Culture, and seedling-growing container is covered with the fly net of 40 mesh, it waters in due course according to routine techniques.Day temperature is 28~30 DEG C, night
Temperature is 18~20 DEG C, and humidity is 70%~90%.
Remarks explanation:
Growing way is preferable: the height of seedling reaches 7cm, and blade is emerald green, and it is preferable that stem diameter 2mm or more is considered as growing way；
The experienced seedling is to extract seedling from group piping, cleans root culture medium with tap water, is placed on ventilation (temperature
It is 25 DEG C) it is dried to root；Time is 1h or so.
Watering in due course refers to that when water content≤12%, watering is until water content is 18%.
3), stem section is sheared:
When the seedling of potato plant length to 30cm or so (about 10 days or so), plant top 5cm is cut away with blade, so
Potato plant stem section is cut into the stem section with a piece of compound leaf afterwards, notch is up and down 1cm away from petiole base.It is cut by this step
It cuts growth coefficient and reaches 8~9 times.
4), stem section cuttage:
Cultivation matrix is dispensed into hole tray, is irrigated with water.By the potato plant stem section cut by morphology upper end court
Into hole tray, cuttage depth covers petiole base just by matrix for upper cuttage.It is equally put into net canopy and cultivates；Condition of culture is same
Step 2).
5) micro potato, is harvested:
After about 3 weeks (21 days), the micro potato of about 1g weight is born at the leaf segment of the potato stem section of cuttage, as shown in Figure 1, will
Micro potato, such as Fig. 2 are extracted and harvested to stem section.
6), matrix is dispensed into seedling-growing container, is irrigated with water.By the micro potato kind of harvest into seedling-growing container.It puts
It is cultivated in networking canopy.The same step 2 of condition of culture.
7), about after a week, the micro potato of harvest grows up to seedling, as described in Figure 3, when seedling grows to 30cm or so
(about needing 10 days) repeats the seedling in above-mentioned cuttage step (that is, step of replacing 3), and continues implementation steps 4) and 5), obtain
Micro potato), this step can expand numerous 8~9 times again.
Potato Cultivars in embodiment 1 are changed to " middle potato 3 " by " cherie " micro potato by embodiment 2；Remaining is equivalent
In embodiment 1.
As a result are as follows:
Step 3): it when potato plant length to 30cm or so, about needs 10 days；Growth coefficient is 9~10 times.
Step 5): about 18 days, the micro potato of about 1g is born at the leaf segment of the potato stem section of cuttage；
Step 7): about 7 days, the micro potato of harvest grew up to seedling；30cm or so is grown to seedling, is about needed 9 days；Expand
Numerous multiple is 9~10 times.
Potato Cultivars in embodiment 1 are changed to " river 56 " by " cherie " micro potato by embodiment 3；Remaining is equal to
Embodiment 1.
As a result are as follows:
Step 3): it when potato plant length to 30cm or so, about needs 9 days；Growth coefficient is 7~8 times.
Step 5): about 19 days, the micro potato of about 1g weight is born at the leaf segment of the potato stem section of cuttage；
Step 7): about 7 days, the micro potato of harvest grew up to seedling；30cm or so is grown to seedling, is about needed 10 days；
Expanding numerous multiple is 7~8 times.
Comparative example 1-1, by the turf of embodiment 1: vermiculite: perlite is changed to " 2:1:1 " by 1:1:1, remaining is equal to reality
Apply example 1.
As a result are as follows:
Step 3): when potato plant length to 30cm or so, about 13 days；Growth coefficient is 6~7 times.
Step 5): about 30 days, the micro potato of about 1g is born at the leaf segment of the potato stem section of cuttage；
Step 7): about 9 days, the micro potato of harvest grew up to seedling；30cm or so is grown to seedling, is about needed 12 days；
Expanding numerous multiple is 6~7 times.
Comparative example 1-2, conventional substrate --- the turf that the cultivation matrix of embodiment 1 is changed to current potato culture.Its
It is remaining to be equal to embodiment 1.
As a result are as follows:
Step 3): when potato plant length to 30cm or so, about 15 days；Growth coefficient is 4~5 times.
Step 5): about 26 days, the micro potato of about 1g weight is born at the leaf segment of the potato stem section of cuttage；
Step 7): about 10 days, the micro potato of harvest grew up to seedling；30cm or so is grown to seedling, is about needed 14 days；
Expanding numerous multiple is 4~5 times.
The upper and lower notch of stem section in 1 step 3) of embodiment away from petiole base is that 1 ± 0.1cm is changed to by comparative example 2
" upper cut of stem section is 0.5 ± 0.1cm away from petiole base, and the lower cut of stem section is 1 ± 0.1cm " away from petiole base；Remaining etc.
It is same as embodiment 1.
As a result are as follows:
Step 5): about 24 days, the micro potato of about 1g is born at the leaf segment of the potato stem section of cuttage.
The above list is only a few specific embodiments of the present invention for finally, it should also be noted that.Obviously, this hair
Bright to be not limited to above embodiments, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (1)
1. using the method for potato stem section numerous micro potato fastly, it is characterized in that successively the following steps are included:
1), the preparation of cultivation matrix:
Turf, vermiculite, perlite are mixed by the mass ratio of 1:1:1, are configured to cultivation matrix；
2), test tube transplantation of seedlings:
The resulting cultivation matrix of step 1) is dispensed into seedling-growing container, after being irrigated with water, transplants the potato set of a height of 7 ~ 8 cm；
It is then placed in culture in the net canopy equipped with sunshade net and insect preventing net cover, is watered in due course in incubation, control day temperature is 28 ~
30 DEG C, nocturnal temperature is 18 ~ 20 DEG C, and relative humidity is 70% ~ 90%；
3), stem section is sheared:
When the plant of step 2 culture potato it is long to 28 ~ 32cm when, 4 ~ 6cm of plant top is cut away, then by potato plant
Stem section is cut into the stem section with a piece of compound leaf, and the upper and lower notch of stem section is 1 ± 0.1cm away from petiole base；
4), stem section cuttage:
The resulting cultivation matrix of step 1) is dispensed into hole tray, is irrigated with water；
Step 3) potato plant stem section obtained is cultivated by the cuttage upward of morphology upper end into hole tray, when cuttage
Cover petiole base just by cultivation matrix；The same step 2 of condition of culture；
5) micro potato, is harvested:
After bearing the micro potato of 0.8 ~ 1.2g weight at the leaf segment of the potato stem section of cuttage in step 4), and this stem section is pulled out
Out, and micro potato is harvested.
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| CN201610917784.3A CN106561210B (en) | 2016-10-21 | 2016-10-21 | Utilize the method for potato stem section numerous micro potato fastly |
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| CN201610917784.3A CN106561210B (en) | 2016-10-21 | 2016-10-21 | Utilize the method for potato stem section numerous micro potato fastly |
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| CN111528097B (en) * | 2020-06-09 | 2023-03-21 | 吉林省蔬菜花卉科学研究院 | Method for breeding potato mini-potatoes by utilizing stem segments of potato stolons |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1698414A (en) * | 2005-07-07 | 2005-11-23 | 中国科学院新疆生态与地理研究所 | Secondary shearing method for de-viruses potato germchit |
| CN102057834A (en) * | 2010-11-05 | 2011-05-18 | 王梦飞 | Method for rapidly breeding mini-potatoes by utilizing in-vitro axillary buds of potatoes |
| CN105766574A (en) * | 2016-03-10 | 2016-07-20 | 李文超 | Method for utilizing test tube potatoes for efficiently producing detoxication breeder's seeds |
| KR20160088671A (en) * | 2015-01-16 | 2016-07-26 | 이기석 | Method for Growing Sweet Potato |
-
2016
- 2016-10-21 CN CN201610917784.3A patent/CN106561210B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1698414A (en) * | 2005-07-07 | 2005-11-23 | 中国科学院新疆生态与地理研究所 | Secondary shearing method for de-viruses potato germchit |
| CN102057834A (en) * | 2010-11-05 | 2011-05-18 | 王梦飞 | Method for rapidly breeding mini-potatoes by utilizing in-vitro axillary buds of potatoes |
| KR20160088671A (en) * | 2015-01-16 | 2016-07-26 | 이기석 | Method for Growing Sweet Potato |
| CN105766574A (en) * | 2016-03-10 | 2016-07-20 | 李文超 | Method for utilizing test tube potatoes for efficiently producing detoxication breeder's seeds |
Non-Patent Citations (3)
| Title |
|---|
| 南方地区春马铃薯茎段扦插实用技术效果;赵文静 等;《长江蔬菜》;20071231(第9期);第53-55页 * |
| 马铃薯脱毒微型薯生产与利用;裴荣信;《山西农业科学》;19921231(第1期);第14-15页 * |
| 马铃薯脱毒苗不同茎段扦插苗生产原原种效果研究初报;龙国 等;《种子》;20080731;第27卷(第7期);第75-76页 * |
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