CN1698414A - Secondary shearing method for virus-free potato seedlings - Google Patents

Abstract

The invention relates to a secondary shearing method for de-viruses potato germchit which comprises transplanting the test tube seedling, employing the breeding techniques of shearing, cutting and enlarging toxic seeds sprout to the fitness sprouts. The disclosed method can diminish the complex procedure of indoor test tube sprout breeding, it is time- and labor-conserving.

Description

Secondary shearing method for virus-free potato seedlings

Technical field

The invention relates to a secondary shearing method for virus-free potato seedlings.

Background technique

Potato is one of the important cultivated crops among non-cereal crops. It has the characteristics of high yield, early maturation, multiple uses, wide distribution, low cultivation seasonal requirements, and both vegetable and grain. It is one of the favorite vegetables in people's daily life.

However, due to the harm of potato virus disease, the yield and quality of potato are seriously affected. A large number of experimental studies have proved that viruses are the main cause of degeneration. In order to solve the problem of potato degeneration, plant biotechnology—potato stem tip virus-free tissue culture technology is mainly used at present to obtain potato disease-free seedlings for planting, which can increase yield by 30% to more than double.

To plant virus-free potatoes, it is first necessary to use test-tube seedling culture technology to propagate virus-free seedlings in large quantities indoors. The test-tube seedlings are then transplanted to room temperature or in a net room to produce virus-free small seed potatoes (original species), and then propagated through the breeding system to produce virus-free potatoes. Poisonous seed potato. In the virus-free potato production system, mass propagation of test-tube plantlets is essential. However, the reproductive technology of test-tube seedlings requires high requirements and many steps. From the preparation of medium, sterilization, repeated cutting of test-tube seedlings under aseptic operation, cultivating test-tube seedlings under suitable temperature and light, until transplanting in greenhouses or net rooms . The whole process is cumbersome, time-consuming and expensive. A little negligence will result in a large amount of contamination and waste of test tube seedlings.

The present invention aims at the current existing problems. After many years of research and experimentation, after the test-tube seedlings are transplanted, the healthy seedlings are cut and cut to increase the reproduction quantity of the virus-free seedlings and reduce the indoor propagation process of the test-tube seedlings. The indoor propagation of virus-free potato plantlets in test tubes is called the first shear propagation, and the shearing after transplanting is called the second shear propagation.

Contents of the invention

The object of the present invention is to provide a secondary shearing method for virus-free potato seedlings. The method is to cut and cut robust seedlings after transplanting test-tube seedlings to expand the number of virus-free seedlings and reduce The cumbersome process of indoor propagation of test tube seedlings saves labor and time, and reduces consumption. Practice has proved that the survival rate of cuttage secondary cut seedlings reaches 95%-98%, and the seedlings grow fast and robust.

The secondary shearing method of potato virus-free seedling of the present invention, carries out according to the following steps:

a. After transplanting the potato test-tube seedlings into the greenhouse or net room, after 22-27 days of cultivation, the seedlings can be cut and cut when they have grown 7-8 leaves;

b. When cutting, keep 1-2 leaves on the ground, and remove the leaves from the part inserted into the matrix. The depth of insertion accounts for 1/2 of the length of the cut section;

c. When cutting, the seedbed should be prepared in advance, and 8-10cm of forest soil or peat soil should be laid in the seedbed as the cutting substrate. Before cutting, it should be watered thoroughly, and the tools used should be routinely disinfected;

d. During the cutting process, a part of the cuttings should be immediately covered with a small white film shed to keep it moist. After the cuttage of a plot is completed, water should be sprayed once, and all the sheds should be kept moist. The humidity in the shed is 100%, and the temperature is 25-28°C. After 7-8 When the young seedlings grow new leaves or the side buds germinate, gradually remove the film of the small arch shed for 2-3 days, then remove all the small arch shed, cut the cuttings at a density of 200 plants/m ² , spray once from the seedling stage to before budding Foliar fertilizer is enough.

Detailed ways

Example 1

a. After the potato tube seedlings are transplanted into a temperature-humidity-controllable greenhouse, after 22 days of cultivation, the seedlings can be cut and cut when they have grown 7 leaves.

b. When cutting, keep 1 leaf on the aboveground part, and remove the leaf from the part inserted into the substrate. The depth of insertion accounts for 1/2 of the length of the cut section;

c. When cutting, the seedbed should be prepared in advance, and 8cm of forest soil should be laid in the seedbed as the cutting substrate. Before cutting, it should be watered thoroughly, and the tools used should be routinely disinfected;

d. During the cutting process, immediately place a small white film shed on the cuttage to keep it moist, and spray water once after the cuttage of a plot is completed, and put the whole shed to keep it moist. The humidity in the shed is 100%, and the temperature is 28°C. After 7 days, the seedlings grow out When new leaves or side buds sprout, the small sheds are gradually uncovered and ventilated for 3 days, then all the small sheds are removed, the cutting density of cut sections is 200 plants/m ² , and the foliar fertilizer is sprayed once from the seedling stage to before budding. The survival rate of cuttage secondary cut seedlings is 98%. According to the growing season, it can also be cut 2-3 times to expand the reproduction number of virus-free seedlings.

Example 2

a. After the potato tube seedlings are transplanted into the net room, the cuttings can be cut when the seedlings have grown 8 leaves after 27 days of cultivation.

b. When cutting, keep 2 leaves on the aboveground part, and remove the leaves from the part inserted into the substrate. The depth of insertion accounts for 1/2 of the length of the cut section;

c. When cutting, the seedbed should be prepared in advance, and 10cm of peat soil should be laid in the seedbed as the cutting substrate. Before cutting, it should be watered thoroughly, and the tools used should be routinely disinfected;

d. During the cutting process, a part of the cuttings should be immediately covered with a small white film shed to keep it moist. After the cuttage of a plot is completed, water should be sprayed once, and the whole shed should be covered to keep it moisturized. The humidity in the shed is 100%, and the temperature is 25°C. After 8 days, the seedlings will grow When new leaves or lateral buds sprout, gradually remove the film of the small shed for 2 days, then remove all the small shed, cut the cuttings at a density of 200 plants/m ² , and spray foliar fertilizer once from the seedling stage to before budding. The survival rate of cuttage secondary cut seedlings reaches 95%. According to the growing season, it can also be cut 2-3 times to expand the reproduction number of virus-free seedlings.

Claims (1)

1, a kind of secondary shearing method of virus-free potato seedling, it is characterized in that the method is carried out according to the following steps: a. After transplanting the potato test-tube seedlings into the greenhouse or net room, after 22-27 days of cultivation, the seedlings can be cut and cut when they have grown 7-8 leaves; b. When cutting, keep 1-2 leaves on the ground, and remove the leaves from the part inserted into the matrix. The depth of insertion accounts for 1/2 of the length of the cut section; c. When cutting, the seedbed should be prepared in advance, and 8-10cm of forest soil or peat soil should be laid in the seedbed as the cutting substrate. Before cutting, it should be watered thoroughly, and the tools used should be routinely disinfected; d. During the cutting process, a part of the cuttings should be immediately covered with a small white film shed to keep it moist. After the cuttage of a plot is completed, water should be sprayed once, and all the sheds should be kept moist. The humidity in the shed is 100%, and the temperature is 25-28°C. After 7-8 When the young seedlings grow new leaves or the side buds germinate, gradually remove the film of the small arch shed for 2-3 days, then remove all the small arch shed, cut the cuttings at a density of 200 plants/m ² , spray once from the seedling stage to before budding Foliar fertilizer is enough.