CN1330234C - Secondary shearing method for de-viruses potato germchit - Google Patents
Secondary shearing method for de-viruses potato germchit Download PDFInfo
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- CN1330234C CN1330234C CNB200510083424XA CN200510083424A CN1330234C CN 1330234 C CN1330234 C CN 1330234C CN B200510083424X A CNB200510083424X A CN B200510083424XA CN 200510083424 A CN200510083424 A CN 200510083424A CN 1330234 C CN1330234 C CN 1330234C
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Abstract
The present invention relates to a secondary shearing method of virus-free potato seedlings. In the method, after test-tube seedlings are transplanted, through the propagation technique of shearing and cutting on healthy seedlings to enlarge virus-free seedlings, the complicated processes of breeding test-tube seedlings indoors are reduced to save labor and time and reduce consumption. Practice proves that seedlings through cutting and secondary shearing have a survival rate of 95% to 98% and can be sheared for two or three times to enlarge the breeding quantity of virus-free seedlings according to growing season situations to grow fast and strongly.
Description
Technical field
The present invention relates to a kind of secondary shearing method of potato virus-free seedling.
Background technology
One of important raise crop in the potato right and wrong cereal has high yield, precocity, characteristics such as purposes is many, distribution is wide, the cultivation season requirement is strong, dish grain dual-purpose, is one of vegetables of liking in people's daily life.
But,, have a strong impact on the yield and quality of potato owing to the harm of potato virus disease.Prove that through a large amount of experimental studies virus is the main cause that causes degeneration.In order to solve the problem of potato degeneration, the main at present Plant Biotechnology that adopts---potato stem apex detoxification tissue culture technique acquisition potato takes off the disease seedling to be planted, and can increase production more than 30% to one times.
The plantation toxicity-removing white potato at first must be bred the virus-free seedling in a large number in indoor application test-tube plantlet culture technique, and test-tube plantlet is transplanted to room temperature again or the solarium produces virus-free small seed potato (breeder's stock), is expanding numerous production virus-free seed potato through breeding of method.In the toxicity-removing white potato production system, a large amount of breedings of test-tube plantlet are absolutely necessary.But that the propagation technique of test-tube plantlet requires is high, step is many, under culture medium preparation, sterilization, the sterile working test-tube plantlet cut repeatedly numerous, under preference temperature, illumination the culture test tube seedling, up to intermediate house or solarium.Whole process is more loaded down with trivial details, and is time-consuming, consumes big.There is carelessness will cause a large amount of test-tube plantlets to pollute waste slightly.
The present invention is directed to the problem of present existence, through years of researches tests, behind test-tube seedling transplanting, adopt to healthy seedling shear, cuttage to be to enlarge the breeding quantity of virus-elimination seedlings, reduces indoor propagation test-tube plantlet reproductive process.Toxicity-removing white potato test-tube plantlet indoor propagation is called shears breeding for the first time, shears after transplanting to be called and shears breeding for the second time.
Summary of the invention
The object of the invention is, a kind of secondary shearing method of potato virus-free seedling is provided, and this method is with behind the test-tube seedling transplanting, employing to healthy seedling shear, cuttage to be to enlarge the breeding quantity of virus-elimination seedlings, reduce indoor propagation test-tube plantlet complicated processes, saving of work and time reduces consuming.Facts have proved that the survival rate of cuttage secondary shearing seedling reaches 95%-98%, the seedling growth is fast healthy and strong.
The secondary shearing method of potato virus-free seedling of the present invention follows these steps to carry out:
A, with behind potato test-tube plantlet intermediate house or the solarium, when 22-27 days cultivation seedling has been grown 7-8 blade, can shear cuttage;
Overground part keeps 1-2 blade when b, cuttage, inserts the part of matrix and removes blade, and the degree of depth of insertion accounts for 1/2 of staple fiber ppd;
Will be ready to the seedbed in advance when c, cuttage, forest soil or the turfy soil of shop 8-10cm are made cutting medium in the seedbed, will water permeablely before inserting, and employed instrument are carried out routine disinfection;
D, in the cuttage process, a cuttage part is just detained the little shed of white films immediately and is preserved moisture, after finishing, a sub-district cuttage sprays a water, all the button canopy is preserved moisture, and humidity is 100% in the canopy, and temperature is 25-28 ℃, when seedling grew young leaves or surveys the bud germinating through 7-8 days, little shed is taken off film gradually ventilate exercise after 2-3 days, little shed is all removed, the segment cutting density is 200 strains/m
2, spray a foliage fertilizer before budding and get final product seedling stage.
Embodiment
Embodiment 1
A, potato test-tube plantlet transplanted the regulatable greenhouse of temp. and humidity after, when 22 days cultivation seedling has been grown 7 blades, can shear cuttage, segment of stem top or stem shearing length are as the criterion to be with two buds;
Overground part keeps 1 blade when b, cuttage, inserts the part of matrix and removes blade, and the degree of depth of insertion accounts for 1/2 of staple fiber ppd;
Will be ready to the seedbed in advance when c, cuttage, the forest soil of shop 8cm is made cutting medium in the seedbed, will water permeablely before inserting, and employed instrument is carried out routine disinfection;
D, in the cuttage process, a cuttage part is just detained the little shed of white films immediately and is preserved moisture, after finishing, a sub-district cuttage sprays a water, all the button canopy is preserved moisture, and humidity is 100% in the canopy, and temperature is 28 ℃, when seedling grew young leaves or surveys the bud germinating through 7 days, little shed is taken off film gradually ventilate exercise after 3 days, little shed is all removed, the segment cutting density is 200 strains/m
2, spray a foliage fertilizer before budding and get final product seedling stage, and the survival rate of cuttage secondary shearing seedling reaches 98%.According to season of growth situation, also can shear 2-3 time to enlarge the breeding quantity of virus-elimination seedlings.
Embodiment 2
A, potato test-tube plantlet transplanted the solarium after, when 27 days cultivation seedling has been grown 8 blades, can shear cuttage, segment of stem top or stem shearing length are as the criterion to be with three buds;
Overground part keeps 2 blades when b, cuttage, inserts the part of matrix and removes blade, and the degree of depth of insertion accounts for 1/2 of staple fiber ppd;
Will be ready to the seedbed in advance when c, cuttage, the turfy soil of shop 10cm is made cutting medium in the seedbed, will water permeablely before inserting, and employed instrument is carried out routine disinfection;
D, in the cuttage process, a cuttage part is just detained the little shed of white films immediately and is preserved moisture, after finishing, a sub-district cuttage sprays a water, all the button canopy is preserved moisture, and humidity is 100% in the canopy, and temperature is 25 ℃, when seedling grew young leaves or surveys the bud germinating through 8 days, little shed is taken off film gradually ventilate exercise after 2 days, little shed is all removed, the segment cutting density is 200 strains/m
2, get final product to preceding spray a foliage fertilizer to buddingging seedling stage, and the survival rate of cuttage secondary shearing seedling reaches 95%.According to season of growth situation, also can shear 2-3 time to enlarge the breeding quantity of virus-elimination seedlings.
Claims (1)
1, a kind of secondary shearing method of potato virus-free seedling is characterized in that this method follows these steps to carry out:
A, with behind potato test-tube plantlet intermediate house or the solarium, when 22-27 days cultivation seedling has been grown 7-8 blade, can shear cuttage, segment of stem top or stem shearing length are as the criterion to be with two or three buds;
Overground part keeps 1-2 blade when b, cuttage, inserts the part of matrix and removes blade, and the degree of depth of insertion accounts for 1/2 of staple fiber ppd;
Will be ready to the seedbed in advance when c, cuttage, forest soil or the turfy soil of shop 8-10cm are made cutting medium in the seedbed, will water permeablely before inserting, and employed instrument are carried out routine disinfection;
D, in the cuttage process, a cuttage part is just detained the little shed of white films immediately and is preserved moisture, after finishing, a sub-district cuttage sprays a water, all the button canopy is preserved moisture, and humidity is 100% in the canopy, and temperature is 25-28 ℃, when seedling grew young leaves or lateral bud germinating through 7-8 days, little shed is taken off film gradually ventilate exercise after 2-3 days, little shed is all removed, the segment cutting density is 200 strains/m
2, spray a foliage fertilizer before budding and get final product seedling stage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510083424XA CN1330234C (en) | 2005-07-07 | 2005-07-07 | Secondary shearing method for de-viruses potato germchit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510083424XA CN1330234C (en) | 2005-07-07 | 2005-07-07 | Secondary shearing method for de-viruses potato germchit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1698414A CN1698414A (en) | 2005-11-23 |
| CN1330234C true CN1330234C (en) | 2007-08-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200510083424XA Expired - Fee Related CN1330234C (en) | 2005-07-07 | 2005-07-07 | Secondary shearing method for de-viruses potato germchit |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054527A (en) * | 2013-03-22 | 2014-09-24 | 林华善 | Organic vegetable cultivation device |
| CN104429866B (en) * | 2014-12-24 | 2016-08-24 | 延边大学 | A kind of micro potato production method germinateed without dormancy |
| CN106665024A (en) * | 2015-11-10 | 2017-05-17 | 祁海军 | Simple cuttage method for virus-free potato breeding |
| CN106561210B (en) * | 2016-10-21 | 2019-11-05 | 浙江大学 | Utilize the method for potato stem section numerous micro potato fastly |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1089076A (en) * | 1993-01-07 | 1994-07-13 | 天津市蔬菜研究所 | Fast propagation technique for potato using depoisoned small seed-potato |
| CN1158691A (en) * | 1997-03-10 | 1997-09-10 | 大港油田集团运输公司 | Production method and cultivation equipment for miniature detoxicated potato seeds |
| CN1348990A (en) * | 2001-12-11 | 2002-05-15 | 中国科学院石家庄农业现代化研究所 | Culture medium for tissue culture of devirused potato and its prepn |
-
2005
- 2005-07-07 CN CNB200510083424XA patent/CN1330234C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1089076A (en) * | 1993-01-07 | 1994-07-13 | 天津市蔬菜研究所 | Fast propagation technique for potato using depoisoned small seed-potato |
| CN1158691A (en) * | 1997-03-10 | 1997-09-10 | 大港油田集团运输公司 | Production method and cultivation equipment for miniature detoxicated potato seeds |
| CN1348990A (en) * | 2001-12-11 | 2002-05-15 | 中国科学院石家庄农业现代化研究所 | Culture medium for tissue culture of devirused potato and its prepn |
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| CN1698414A (en) | 2005-11-23 |
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