CN106119322A - 一种提高重组人源胶原蛋白生产水平的发酵工艺 - Google Patents
一种提高重组人源胶原蛋白生产水平的发酵工艺 Download PDFInfo
- Publication number
- CN106119322A CN106119322A CN201610587403.XA CN201610587403A CN106119322A CN 106119322 A CN106119322 A CN 106119322A CN 201610587403 A CN201610587403 A CN 201610587403A CN 106119322 A CN106119322 A CN 106119322A
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- Prior art keywords
- fermentation
- collagen protein
- recombination human
- human source
- sodium pyruvate
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Links
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- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 21
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- 229940054269 sodium pyruvate Drugs 0.000 claims abstract description 13
- 241000235061 Pichia sp. Species 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 102
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 26
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- 241000235058 Komagataella pastoris Species 0.000 claims description 9
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 229910021529 ammonia Inorganic materials 0.000 claims description 2
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- 229910052799 carbon Inorganic materials 0.000 description 16
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 12
- 239000002054 inoculum Substances 0.000 description 9
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 8
- 239000002585 base Substances 0.000 description 8
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 7
- 239000002253 acid Substances 0.000 description 7
- 229920001436 collagen Polymers 0.000 description 7
- 229910052708 sodium Inorganic materials 0.000 description 7
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- 229940047431 recombinate Drugs 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
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- 241000235648 Pichia Species 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- 235000014683 Hansenula anomala Nutrition 0.000 description 1
- 229910018890 NaMoO4 Inorganic materials 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
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- 235000020958 biotin Nutrition 0.000 description 1
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- 238000001311 chemical methods and process Methods 0.000 description 1
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- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
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- 230000012010 growth Effects 0.000 description 1
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- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 150000004715 keto acids Chemical class 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
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- 235000011007 phosphoric acid Nutrition 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (6)
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610587403.XA CN106119322B (zh) | 2016-07-22 | 2016-07-22 | 一种提高重组人源胶原蛋白生产水平的发酵工艺 |
KR1020197001826A KR102132132B1 (ko) | 2016-07-22 | 2016-10-18 | 재조합 인간 콜라겐 생산 수준을 향상시키는 발효 공법 |
EP16909380.4A EP3473726B8 (en) | 2016-07-22 | 2016-10-18 | Fermentation technique to improve production level of recombinant human collagen |
PCT/CN2016/102435 WO2018014453A1 (zh) | 2016-07-22 | 2016-10-18 | 一种提高重组人源胶原蛋白生产水平的发酵工艺 |
US16/318,556 US11136373B2 (en) | 2016-07-22 | 2016-10-18 | Fermentation process for increasing production level of recombinant human collagen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610587403.XA CN106119322B (zh) | 2016-07-22 | 2016-07-22 | 一种提高重组人源胶原蛋白生产水平的发酵工艺 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106119322A true CN106119322A (zh) | 2016-11-16 |
CN106119322B CN106119322B (zh) | 2019-10-15 |
Family
ID=57289422
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610587403.XA Active CN106119322B (zh) | 2016-07-22 | 2016-07-22 | 一种提高重组人源胶原蛋白生产水平的发酵工艺 |
Country Status (5)
Country | Link |
---|---|
US (1) | US11136373B2 (zh) |
EP (1) | EP3473726B8 (zh) |
KR (1) | KR102132132B1 (zh) |
CN (1) | CN106119322B (zh) |
WO (1) | WO2018014453A1 (zh) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107988293A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 调节压力提高重组人源胶原蛋白生产水平的发酵工艺 |
CN107988294A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 调节温度提高重组人源胶原蛋白生产水平的发酵工艺 |
CN107988291A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 毕赤酵母表达重组人源胶原蛋白的甲醇流加发酵工艺 |
CN107988292A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 提高重组人源胶原蛋白稳定性的发酵工艺 |
CN108048511A (zh) * | 2018-01-18 | 2018-05-18 | 江苏江山聚源生物技术有限公司 | 调节pH提升重组人源胶原蛋白生产水平的发酵工艺 |
CN113564062A (zh) * | 2021-09-10 | 2021-10-29 | 汉肽生物医药集团有限公司 | 一种缩短培养时间和提高胶原蛋白含量的发酵工艺 |
CN114045321A (zh) * | 2021-12-24 | 2022-02-15 | 江苏江山聚源生物技术有限公司 | 谷胱甘肽在发酵生产重组人源胶原蛋白中的应用 |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11180541B2 (en) | 2017-09-28 | 2021-11-23 | Geltor, Inc. | Recombinant collagen and elastin molecules and uses thereof |
CN109680025B (zh) * | 2018-12-25 | 2022-05-13 | 江苏江山聚源生物技术有限公司 | 提高重组人源胶原蛋白生产水平且降低蛋白降解速度的发酵工艺 |
US11168126B2 (en) | 2019-04-12 | 2021-11-09 | Geltor, Inc. | Recombinant elastin and production thereof |
CN115298212A (zh) | 2020-01-24 | 2022-11-04 | 格尔托公司 | 无动物膳食胶原蛋白 |
CN111534444B (zh) * | 2020-03-19 | 2023-11-14 | 山西大禹生物工程股份有限公司 | 高密度培养毕赤酵母流加盐的方法 |
CN111733200A (zh) * | 2020-06-30 | 2020-10-02 | 浙江诸暨聚源生物技术有限公司 | 调节ptm1添加方式提高重组胶原蛋白生产水平的发酵工艺 |
CN113667709B (zh) * | 2021-08-28 | 2023-11-21 | 西安德诺海思医疗科技有限公司 | 一种重组人源化胶原蛋白的发酵方法 |
CN114159341B (zh) * | 2021-12-22 | 2023-07-25 | 江苏聚源医疗技术有限公司 | 一种冻干眼贴及其制备方法 |
CN115074393B (zh) * | 2022-08-04 | 2023-11-21 | 江苏创健医疗科技股份有限公司 | 一种巴斯德毕赤酵母发酵溶胞物滤液、制备方法及应用 |
CN117025655B (zh) * | 2023-07-11 | 2024-02-27 | 山东丰金美业科技有限公司 | 一种提高人源ⅲ型重组胶原蛋白产量的基因表达载体、基因工程菌及方法和应用 |
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CN101570771A (zh) * | 2009-06-09 | 2009-11-04 | 华东理工大学 | 一种毕赤酵母基因工程菌发酵生产s-腺苷甲硫氨酸的方法 |
ITGE20130040A1 (it) | 2013-04-18 | 2014-10-19 | Univ Degli Studi Genova | Metodo per la produzione di collagene marino ricombinante e organismo capace di produrre detto collagene marino |
CN103725623A (zh) | 2013-12-19 | 2014-04-16 | 西安巨子生物基因技术股份有限公司 | 一种分泌表达人Ⅲ型胶原α链蛋白的毕赤酵母工程菌及其构建方法与应用 |
US10294482B2 (en) | 2014-05-22 | 2019-05-21 | Yeda Research And Development Co. Ltd. | Recombinant microorganisms capable of carbon fixation |
CN104561201A (zh) | 2014-12-29 | 2015-04-29 | 山东鲁抗医药股份有限公司 | 膜过滤法提取重组类人胶原蛋白的方法 |
WO2017033965A1 (ja) * | 2015-08-24 | 2017-03-02 | 国立研究開発法人理化学研究所 | 芳香族化合物及びその誘導体の製造方法 |
-
2016
- 2016-07-22 CN CN201610587403.XA patent/CN106119322B/zh active Active
- 2016-10-18 EP EP16909380.4A patent/EP3473726B8/en active Active
- 2016-10-18 KR KR1020197001826A patent/KR102132132B1/ko active IP Right Grant
- 2016-10-18 WO PCT/CN2016/102435 patent/WO2018014453A1/zh unknown
- 2016-10-18 US US16/318,556 patent/US11136373B2/en active Active
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CN102443057A (zh) * | 2011-10-26 | 2012-05-09 | 南京理工大学 | 一种重组人源胶原蛋白及其制备方法 |
Non-Patent Citations (4)
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NOCON J,ET AL.: ""Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production"", 《METABOLIC ENGINEERIN》 * |
XIAOXUAN M.ET AL: ""An approach for enhancing the production of human-like collagen II by enlarging the metabolic flux at pyruvate node"", 《PAK J PHARM SCI.》 * |
张敏睿等: "丙型肝炎病毒核心蛋白在大肠杆菌和", 《微生物学通报》 * |
高敏杰等: "重组毕赤酵母生产外源蛋白的过程控制", 《食品工业科技》 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107988293A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 调节压力提高重组人源胶原蛋白生产水平的发酵工艺 |
CN107988294A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 调节温度提高重组人源胶原蛋白生产水平的发酵工艺 |
CN107988291A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 毕赤酵母表达重组人源胶原蛋白的甲醇流加发酵工艺 |
CN107988292A (zh) * | 2018-01-18 | 2018-05-04 | 江苏江山聚源生物技术有限公司 | 提高重组人源胶原蛋白稳定性的发酵工艺 |
CN108048511A (zh) * | 2018-01-18 | 2018-05-18 | 江苏江山聚源生物技术有限公司 | 调节pH提升重组人源胶原蛋白生产水平的发酵工艺 |
CN107988291B (zh) * | 2018-01-18 | 2019-10-15 | 江苏江山聚源生物技术有限公司 | 毕赤酵母表达重组人源胶原蛋白的甲醇流加发酵工艺 |
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CN108048511B (zh) * | 2018-01-18 | 2020-08-11 | 江苏江山聚源生物技术有限公司 | 调节pH提升重组人源胶原蛋白生产水平的发酵工艺 |
CN107988292B (zh) * | 2018-01-18 | 2023-12-26 | 江苏江山聚源生物技术有限公司 | 提高重组人源胶原蛋白稳定性的发酵工艺 |
CN113564062A (zh) * | 2021-09-10 | 2021-10-29 | 汉肽生物医药集团有限公司 | 一种缩短培养时间和提高胶原蛋白含量的发酵工艺 |
CN114045321A (zh) * | 2021-12-24 | 2022-02-15 | 江苏江山聚源生物技术有限公司 | 谷胱甘肽在发酵生产重组人源胶原蛋白中的应用 |
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EP3473726A1 (en) | 2019-04-24 |
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WO2018014453A1 (zh) | 2018-01-25 |
US20190241645A1 (en) | 2019-08-08 |
CN106119322B (zh) | 2019-10-15 |
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