CN105765060B - 羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 - Google Patents

羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 Download PDF

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CN105765060B
CN105765060B CN201480046241.8A CN201480046241A CN105765060B CN 105765060 B CN105765060 B CN 105765060B CN 201480046241 A CN201480046241 A CN 201480046241A CN 105765060 B CN105765060 B CN 105765060B
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mesenchymal
cell
collagenase
mesenchymal cell
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CN105765060A (zh
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山原研一
田口明彦
相马俊裕
大西俊介
小林明
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Hokkaido University NUC
Hyogo College of Medicine
Kaneka Corp
National Cerebral and Cardiovascular Center
Foundation for Biomedical Research and Innovation at Kobe
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Hyogo College of Medicine
Kaneka Corp
National Cerebral and Cardiovascular Center
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CN201480046241.8A 2013-08-19 2014-08-18 羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 Active CN105765060B (zh)

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JP2013170008 2013-08-19
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JP2014130142A JP6512759B2 (ja) 2013-08-19 2014-06-25 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤
PCT/JP2014/071546 WO2015025810A1 (ja) 2013-08-19 2014-08-18 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤

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Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3296392A4 (en) * 2015-05-14 2018-11-21 Advanced Cell Technology and Engineering Ltd. Method for producing frozen mesenchymal cells and method for producing implantable therapeutic member
CN109312302A (zh) * 2015-10-27 2019-02-05 株式会社钟化 包含间充质系干细胞的细胞群的制造方法、间充质系干细胞、细胞群、以及医药组合物
AU2017215955B2 (en) * 2016-02-01 2020-01-23 GC Cell Corporation Medium composition for cryopreservation of cell and use thereof
US11540507B2 (en) 2016-11-04 2023-01-03 The University Of Tokyo Solution for cryopreservation of animal cells or animal tissues, cryopreserved product, and cryopreservation method
WO2018092769A1 (ja) 2016-11-15 2018-05-24 株式会社カネカ 胎児付属物に由来する間葉系幹細胞を含む細胞集団とその製造方法、及び医薬組成物
JP7152389B2 (ja) * 2017-04-03 2022-10-12 株式会社カネカ 間葉系幹細胞を含む細胞集団とその製造方法、間葉系幹細胞及び医薬組成物
JP7140752B2 (ja) * 2017-04-03 2022-09-21 株式会社カネカ 胎児付属物由来接着細胞を含む細胞集団とその製造方法、及び医薬組成物
CN110494556B (zh) * 2017-04-03 2024-04-30 株式会社钟化 包含间充质系干细胞的细胞群、其制造方法、以及医药组合物
CN106967675A (zh) * 2017-05-31 2017-07-21 东莞市保莱生物科技有限公司 一种从胎盘中分离提取造血干细胞的方法
WO2019059279A1 (ja) * 2017-09-20 2019-03-28 株式会社カネカ 細胞懸濁液を調製するためのデバイス、システム及び方法
US20200360444A1 (en) 2017-12-28 2020-11-19 Kaneka Corporation Cell population including adhesive stem cells, production method therefor and pharmaceutical composition
WO2019132026A1 (ja) 2017-12-28 2019-07-04 株式会社カネカ 接着性幹細胞を含む細胞集団とその製造方法、及び医薬組成物
CN108904533A (zh) * 2018-07-04 2018-11-30 卡替(上海)生物技术股份有限公司 牙髓间充质干细胞在制备肝硬化治疗药物中的用途
JPWO2020184350A1 (https=) 2019-03-08 2020-09-17
WO2020251020A1 (ja) * 2019-06-14 2020-12-17 株式会社カネカ 間葉系細胞を含む細胞集団、それを含む医薬組成物、及び、その製造方法
US20230338428A1 (en) * 2020-09-08 2023-10-26 Amit Patel Allogenic umbilical cord stem cells for treating severe respiratory conditions
JP7672647B2 (ja) * 2021-02-05 2025-05-08 株式会社カネカ 間葉系幹細胞を含む細胞集団を含有する軟部組織再生用医薬組成物
JPWO2022203022A1 (https=) * 2021-03-26 2022-09-29
CN113456891B (zh) * 2021-06-16 2022-05-17 成都微沃科技有限公司 一种从细胞层中提取细胞外基质层的方法
CN114097768A (zh) * 2021-11-24 2022-03-01 李敬 一种骨髓干细胞用保护液及其制备方法和应用
CN120400046B (zh) * 2025-07-03 2025-10-17 济南万泉生物技术有限公司 一种羊膜基质细胞培养体系、细胞培养方法及应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002529071A (ja) * 1998-11-09 2002-09-10 コンソルツィオ・ペール・ラ・ジェスティオーネ・デル・チェントロ・ディ・ビオテクノロジー・アヴァンツァテ 軟骨細胞様細胞のための無血清培地
JP2000201672A (ja) 1999-01-11 2000-07-25 Asahi Medical Co Ltd 有核細胞の凍結保存用組成物
JP4330995B2 (ja) 2001-11-15 2009-09-16 チルドレンズ メディカル センター コーポレーション 絨毛膜絨毛、羊水、および胎盤からの胎児性幹細胞を単離、増殖、および分化させる方法、ならびにその治療的使用方法
JP3934539B2 (ja) 2001-12-12 2007-06-20 独立行政法人科学技術振興機構 胎盤等由来の成体又は生後組織の前駆細胞
US7176022B2 (en) * 2002-12-20 2007-02-13 Cell Genesys, Inc. Directly injectable formulations which provide enhanced cryoprotection of cell products
KR20090031895A (ko) 2006-06-09 2009-03-30 안트로제네시스 코포레이션 태반 니치 및 줄기 세포 배양을 위한 이의 용도
NZ612888A (en) 2007-02-12 2015-02-27 Anthrogenesis Corp Treatment of inflammatory diseases using placental stem cells
BRPI0807966A2 (pt) 2007-03-01 2014-06-10 Cryo Cell Int Aquisição, isolação e criopreservação de células endometriais/menstruais
EP2014767A1 (en) * 2007-06-15 2009-01-14 Centro di Ricerca E. Menni T cell immunomodulation by placenta cell preparations
WO2009058365A1 (en) 2007-10-31 2009-05-07 Cryo-Cell International, Inc. Methods for co-culturing cord blood derived cells with menstrual stem cells
KR20200011604A (ko) 2008-08-20 2020-02-03 안트로제네시스 코포레이션 개선된 세포 조성물 및 그의 제조 방법
RU2015130665A (ru) 2008-11-19 2018-12-24 Антродженезис Корпорейшн Амниотические адгезивные клетки
US20120149108A1 (en) * 2009-08-19 2012-06-14 Masashige Tanabe Cell preservation method
EP3351625A1 (en) 2010-02-18 2018-07-25 Osiris Therapeutics, Inc. Methods of manufacture of immunocompatible amniotic membrane products
CA2841713A1 (en) * 2011-07-15 2013-01-24 Anthrogenesis Corporation Treatment of radiation injury using amnion derived adherent cells

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EP3037523A4 (en) 2017-01-25
EP3037523A1 (en) 2016-06-29
JP6512759B2 (ja) 2019-05-15
US10441611B2 (en) 2019-10-15
US20190343892A1 (en) 2019-11-14
JP2015061520A (ja) 2015-04-02
WO2015025810A1 (ja) 2015-02-26
US20160228474A1 (en) 2016-08-11
CN105765060A (zh) 2016-07-13
EP3037523B1 (en) 2020-08-12

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