CN105659961A - Method for increasing transplanting survival rate of polygonum multiflorum tissue culture seedlings - Google Patents

Method for increasing transplanting survival rate of polygonum multiflorum tissue culture seedlings Download PDF

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Publication number
CN105659961A
CN105659961A CN201610010909.4A CN201610010909A CN105659961A CN 105659961 A CN105659961 A CN 105659961A CN 201610010909 A CN201610010909 A CN 201610010909A CN 105659961 A CN105659961 A CN 105659961A
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CN
China
Prior art keywords
survival rate
transplanting survival
cultured seedling
tissue cultured
centimetre
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Pending
Application number
CN201610010909.4A
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Chinese (zh)
Inventor
何志铿
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Gold Blue Collar Education And Science Co Ltd Of Foshan City
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Gold Blue Collar Education And Science Co Ltd Of Foshan City
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Application filed by Gold Blue Collar Education And Science Co Ltd Of Foshan City filed Critical Gold Blue Collar Education And Science Co Ltd Of Foshan City
Priority to CN201610010909.4A priority Critical patent/CN105659961A/en
Publication of CN105659961A publication Critical patent/CN105659961A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention provides a method for increasing the transplanting survival rate of polygonum multiflorum tissue culture seedlings and belongs to the technical field of polygonum multiflorum cultivation. The method comprises the following steps of manure collecting, soil preparation and planting, and the transplanting survival rate is increased starting from manure, the transplanting mode and an additive. The method has the advantages of being high in survival rate, environmentally friendly and low in cost.

Description

A kind of method improving Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate
Technical field
The invention belongs to Tuber Fleeceflower Root cultivation field, in particular to a kind of method improving Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate.
Background technology
Tuber Fleeceflower Root is that polygonaceae Tuber Fleeceflower Root belongs to perennial winding vine, and block root is plump, oblong, chocolate, and its block root is used as medicine, and can calm the nerves, nourish blood, active, disappear carbuncle, is common Chinese medicinal materials.
Current Tuber Fleeceflower Root is bred fast and is adopted tissue culture propagating more, but due to environment acute variation during tissue cultured seedling bottle outlet, and major part tissue cultured seedling is dead because not adapting to the environment of cataclysm, causes Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate low, and greatly waste group trains achievement.
Summary of the invention
There are the problems referred to above based on prior art, the present invention provides a kind of method improving Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate, the method comprises the following steps: collected manure, whole and field planting, starting with from fertilizer, mode of planting and additive three aspect raising transplanting survival rate, method provided by the invention has the advantage of surviving rate height, environmental protection, low cost.
Improving a method for Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate, it comprises the following steps:
Step S10 collects manure, and selects the good sandy soil of draining, hits tight ground, spread one layer of wheat chaff, then by short for hand hay cutter and the stalk of dipped water, the even layer overlay of weeds, compresses, sprinkles and add urea and calcium phosphate after compression, paving one layer of pond sludge on dry straw;
Wholely, soil deeptillage, thin for earth rake leveling, builds high furrow to step S20;
Step S30 field planting, takes out tissue cultured seedling from tissue culture bottle, cuts the downright bad part of brownization, plants on furrow, and apply choline dichloride.
The consumption sprinkling the urea added in step S10 is the 0.1%-1% of dry straw weight, the consumption of calcium phosphate is the 1%-4% of dry straw weight, and above dry straw, pond sludge layer thickness is 5-20 centimetre, adopts urea and calcium phosphate to substitute human excrement and livestock ight soil, reduce distributing of smell, avoid causing environmental pollution.
The degree of depth turning over dark soil in step S20 is 40-50 centimetre, and high furrow width is 100-150 centimetre.
The choline dichloride concentration that step S30 applies is 0.01%-0.1%, and every strain field planting distance is 20-30 centimetre, adds the growth that choline dichloride temporarily suppresses tissue cultured seedling, accumulation nutritive substance, the environment of reply cataclysm.
Embodiment
Below in conjunction with embodiment, a kind of method improving Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate of the present invention is further described.
The sandy soil selecting draining good, hit tight ground, spread one layer of wheat chaff, then by hand hay cutter short and the stalk of dipped water, the even layer overlay of weeds, compress, sprinkle by the 0.5% of dry straw weight after compression and add urea, then by 5% interpolation calcium phosphate of dry straw weight, on dry straw, then spread the pond sludge of one layer of 10 cm thick.
After collected manure, soil deeptillage 40 centimetres, then thin for earth rake leveling, builds the high furrow that width is 100-150 centimetre;
High furrow are cut the earth hole by the field planting distance of 20 centimetres x20 centimetre, Tuber Fleeceflower Root tissue cultured seedling through hardening is taken out from tissue culture bottle, cuts brownization necrosis and flavescence part, plant in the heatable adobe sleeping platform on furrow, and apply the choline dichloride that concentration is 0.5%.
One week statistics surviving rate after transplanting, surviving rate can reach more than 96%, obviously higher than the transplanting survival rate of prior art.

Claims (4)

1. one kind is improved the method for Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate, it is characterised in that, it comprises the following steps:
Step S10 collects manure, and selects the good sandy soil of draining, hits tight ground, spread one layer of wheat chaff, then by short for hand hay cutter and the stalk of dipped water, the even layer overlay of weeds, compresses, sprinkles and add urea and calcium phosphate after compression, paving one layer of pond sludge on dry straw;
Wholely, soil deeptillage, thin for earth rake leveling, builds high furrow to step S20;
Step S30 field planting, takes out tissue cultured seedling from tissue culture bottle, cuts the downright bad part of brownization, plants on furrow, and apply choline dichloride.
2. a kind of method improving Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate according to claim 1, it is characterized in that, the consumption sprinkling the urea added in described step S10 is the 0.1%-1% of dry straw weight, the consumption of calcium phosphate is the 1%-4% of dry straw weight, and above dry straw, pond sludge layer thickness is 5-20 centimetre.
3. a kind of method improving Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate according to claim 1, it is characterised in that, the degree of depth turning over dark soil in described step S20 is 40-50 centimetre, and high furrow width is 100-150 centimetre.
4. a kind of method improving Tuber Fleeceflower Root tissue cultured seedling transplanting survival rate according to claim 1, it is characterised in that, the choline dichloride concentration that described step S30 applies is 0.01%-0.1%, and every strain field planting distance is 20-30 centimetre.
CN201610010909.4A 2016-01-09 2016-01-09 Method for increasing transplanting survival rate of polygonum multiflorum tissue culture seedlings Pending CN105659961A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610010909.4A CN105659961A (en) 2016-01-09 2016-01-09 Method for increasing transplanting survival rate of polygonum multiflorum tissue culture seedlings

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610010909.4A CN105659961A (en) 2016-01-09 2016-01-09 Method for increasing transplanting survival rate of polygonum multiflorum tissue culture seedlings

Publications (1)

Publication Number Publication Date
CN105659961A true CN105659961A (en) 2016-06-15

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610010909.4A Pending CN105659961A (en) 2016-01-09 2016-01-09 Method for increasing transplanting survival rate of polygonum multiflorum tissue culture seedlings

Country Status (1)

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CN (1) CN105659961A (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1408204A (en) * 2002-09-18 2003-04-09 张玉清 Cultivating technology for Mount Taimultiflower knotweed root
CN102960164A (en) * 2012-11-28 2013-03-13 贵州信邦制药股份有限公司 Method for planting polygonum multiflorum
CN104255278A (en) * 2014-10-17 2015-01-07 张家港市鸿嘉数字科技有限公司 Planting technique for tuber fleeceflower root
CN104782358A (en) * 2015-04-09 2015-07-22 安徽裕民生态农业有限公司 Polygonum multiflorum planting method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1408204A (en) * 2002-09-18 2003-04-09 张玉清 Cultivating technology for Mount Taimultiflower knotweed root
CN102960164A (en) * 2012-11-28 2013-03-13 贵州信邦制药股份有限公司 Method for planting polygonum multiflorum
CN104255278A (en) * 2014-10-17 2015-01-07 张家港市鸿嘉数字科技有限公司 Planting technique for tuber fleeceflower root
CN104782358A (en) * 2015-04-09 2015-07-22 安徽裕民生态农业有限公司 Polygonum multiflorum planting method

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Application publication date: 20160615