CN105368809A - 包含蛋白酶变体的组合物和方法 - Google Patents

包含蛋白酶变体的组合物和方法 Download PDF

Info

Publication number
CN105368809A
CN105368809A CN201510671611.3A CN201510671611A CN105368809A CN 105368809 A CN105368809 A CN 105368809A CN 201510671611 A CN201510671611 A CN 201510671611A CN 105368809 A CN105368809 A CN 105368809A
Authority
CN
China
Prior art keywords
amino acid
bpn
sequence
variant
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510671611.3A
Other languages
English (en)
Chinese (zh)
Inventor
J·R·巴斯勒
L·G·卡斯考-佩雷拉
D·A·埃斯特尔
J·T·凯利斯
A·比萨奇科
A·J·保罗斯
D·托雷斯-帕兹米诺
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco USA Inc
Original Assignee
Danisco USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco USA Inc filed Critical Danisco USA Inc
Publication of CN105368809A publication Critical patent/CN105368809A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21062Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Eyeglasses (AREA)
  • Accessory Of Washing/Drying Machine, Commercial Washing/Drying Machine, Other Washing/Drying Machine (AREA)
CN201510671611.3A 2009-12-09 2010-12-09 包含蛋白酶变体的组合物和方法 Pending CN105368809A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US28512709P 2009-12-09 2009-12-09
US61/285,127 2009-12-09
US39237310P 2010-10-12 2010-10-12
US61/392,373 2010-10-12
CN201080063354.0A CN102762222B (zh) 2009-12-09 2010-12-09 包含蛋白酶变体的组合物和方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201080063354.0A Division CN102762222B (zh) 2009-12-09 2010-12-09 包含蛋白酶变体的组合物和方法

Publications (1)

Publication Number Publication Date
CN105368809A true CN105368809A (zh) 2016-03-02

Family

ID=44146173

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201510671611.3A Pending CN105368809A (zh) 2009-12-09 2010-12-09 包含蛋白酶变体的组合物和方法
CN201080063354.0A Active CN102762222B (zh) 2009-12-09 2010-12-09 包含蛋白酶变体的组合物和方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201080063354.0A Active CN102762222B (zh) 2009-12-09 2010-12-09 包含蛋白酶变体的组合物和方法

Country Status (12)

Country Link
US (6) US8728790B2 (https=)
EP (3) EP3599279B1 (https=)
JP (4) JP5863666B2 (https=)
CN (2) CN105368809A (https=)
AR (1) AR079338A1 (https=)
AU (1) AU2010328121A1 (https=)
BR (1) BR112012014082B1 (https=)
CA (1) CA2782891C (https=)
DK (3) DK3190183T3 (https=)
ES (1) ES2746931T3 (https=)
MX (2) MX356282B (https=)
WO (1) WO2011072099A2 (https=)

Families Citing this family (177)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
CN102046783A (zh) * 2008-06-06 2011-05-04 丹尼斯科美国公司 包含变体微生物蛋白酶的组合物和方法
CN102652175B (zh) 2009-12-09 2016-02-10 宝洁公司 织物和家居护理产品
PL2558573T3 (pl) * 2010-04-15 2017-08-31 Danisco Us Inc. Kompozycje i sposoby obejmujące warianty proteazy
JP6448904B2 (ja) * 2010-05-06 2019-01-09 ダニスコ・ユーエス・インク サブチリシン変異体を含む組成物及び方法
WO2012151480A2 (en) * 2011-05-05 2012-11-08 The Procter & Gamble Company Compositions and methods comprising serine protease variants
JP6105560B2 (ja) * 2011-05-05 2017-03-29 ダニスコ・ユーエス・インク セリンプロテアーゼ変異体を含む組成物及び方法
EP2535401B1 (en) 2011-06-17 2017-01-25 Dalli-Werke GmbH & Co. KG Detergent composition comprising soil-release polymers of improved storage stability
US20130123162A1 (en) * 2011-11-10 2013-05-16 The Procter & Gamble Company Consumer products
JP2015504660A (ja) * 2011-12-20 2015-02-16 ノボザイムス アクティーゼルスカブ サブチラーゼ変異体およびそれをコードするポリヌクレオチド
US9234338B2 (en) 2012-09-14 2016-01-12 Impact Products, Llc Fragrance control
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
CN104884614A (zh) 2012-12-21 2015-09-02 丹尼斯科美国公司 α-淀粉酶变体
ES2676895T5 (es) 2013-03-11 2022-04-27 Danisco Us Inc Variantes combinatorias de alfa-amilasa
US20160108388A1 (en) 2013-05-29 2016-04-21 Danisco Us Inc. Novel metalloproteases
WO2014194032A1 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
EP3004342B1 (en) 2013-05-29 2023-01-11 Danisco US Inc. Novel metalloproteases
WO2014194034A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
EP3011020A1 (en) 2013-06-17 2016-04-27 Danisco US Inc. Alpha-amylase from bacillaceae family member
WO2015014803A1 (en) * 2013-07-29 2015-02-05 Novozymes A/S Protease variants and polynucleotides encoding same
CN105358686A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
WO2015050724A1 (en) 2013-10-03 2015-04-09 Danisco Us Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
US20160160199A1 (en) 2013-10-03 2016-06-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
AR098006A1 (es) 2013-10-15 2016-04-27 Danisco Us Inc Gránulo de arcilla
DK3068879T3 (da) 2013-11-14 2020-03-16 Danisco Us Inc Stabile enzymer opnået ved glykeringsreduktion
MX2016006489A (es) 2013-11-20 2016-08-03 Danisco Us Inc Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso.
EP3910057A1 (en) 2013-12-13 2021-11-17 Danisco US Inc. Serine proteases of the bacillus gibsonii-clade
DK3080262T3 (da) 2013-12-13 2019-05-06 Danisco Us Inc Serinproteaser af bacillus-arter
KR20160099629A (ko) 2013-12-16 2016-08-22 이 아이 듀폰 디 네모아 앤드 캄파니 점도 조절제로서의 폴리 알파-1,3-글루칸 에테르의 사용
ES2835703T3 (es) 2013-12-18 2021-06-23 Nutrition & Biosciences Usa 4 Inc Eteres de poli alfa-1,3-glucano catiónicos
WO2015123323A1 (en) 2014-02-14 2015-08-20 E. I. Du Pont De Nemours And Company Poly-alpha-1,3-1,6-glucans for viscosity modification
EP3116914B8 (en) 2014-03-11 2021-04-21 E. I. du Pont de Nemours and Company Oxidized poly alpha-1,3-glucan as detergent builder
US20170096653A1 (en) 2014-03-21 2017-04-06 Danisco Us Inc. Serine proteases of bacillus species
BR112016022447A2 (pt) * 2014-03-28 2017-10-10 Novozymes As ressolubilização de cristais de proteína a ph baixo
EP3158043B1 (en) 2014-06-19 2021-03-10 Nutrition & Biosciences USA 4, Inc. Compositions containing one or more poly alpha-1,3-glucan ether compounds
US9714403B2 (en) 2014-06-19 2017-07-25 E I Du Pont De Nemours And Company Compositions containing one or more poly alpha-1,3-glucan ether compounds
US10138268B2 (en) 2014-10-10 2018-11-27 Enzypep B.V. Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
DK3207129T3 (da) 2014-10-17 2020-02-24 Danisco Us Inc Serinproteaser af bacillus-arten
WO2016065238A1 (en) 2014-10-24 2016-04-28 Danisco Us Inc. Method for producing alcohol by use of a tripeptidyl peptidase
EP3957729A1 (en) 2014-10-27 2022-02-23 Danisco US Inc. Serine proteases
DK3212781T3 (da) 2014-10-27 2019-12-16 Danisco Us Inc Serinproteaser
DK3212662T3 (da) 2014-10-27 2020-07-20 Danisco Us Inc Serinproteaser
EP3212783B1 (en) 2014-10-27 2024-06-26 Danisco US Inc. Serine proteases
CN107148472A (zh) 2014-10-27 2017-09-08 丹尼斯科美国公司 芽孢杆菌属物种的丝氨酸蛋白酶
CN108064306B (zh) 2014-12-23 2022-11-01 营养与生物科学美国4公司 酶促产生的纤维素
CN107835855B (zh) 2015-05-13 2022-05-13 丹尼斯科美国公司 AprL-进化枝蛋白酶变体及其用途
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
FI3307427T3 (fi) 2015-06-09 2023-11-09 Danisco Us Inc Osmoottiset puhkeavat kapselit
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
US11499146B2 (en) 2015-06-17 2022-11-15 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
JP7364330B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス(Paenibacillus)属種及びバチルス(Bacillus)属種のマンナナーゼ
EP4141113A1 (en) 2015-11-05 2023-03-01 Danisco US Inc Paenibacillus sp. mannanases
WO2017083226A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
EP3374401B1 (en) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
JP7045313B2 (ja) 2015-11-13 2022-03-31 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー,インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
WO2017089093A1 (en) 2015-11-25 2017-06-01 Unilever N.V. A liquid detergent composition
DE102015223270A1 (de) * 2015-11-25 2017-06-01 Henkel Ag & Co. Kgaa Proteasevarianten mit verbesserter Enzymstabilität in Wasch- und Reinigungsmitteln
US11920170B2 (en) 2015-12-09 2024-03-05 Danisco Us Inc. Alpha-amylase combinatorial variants
EP3390625B1 (en) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides with endoglucanase activity and uses thereof
CN109071615A (zh) 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
DE102016205670A1 (de) 2016-04-06 2017-10-12 Henkel Ag & Co. Kgaa Neue Protease mit verbesserter Waschleistung
JP2019518440A (ja) 2016-05-03 2019-07-04 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
CN109072213A (zh) 2016-05-05 2018-12-21 丹尼斯科美国公司 蛋白酶变体及其用途
WO2017210295A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
EP3472313B1 (en) * 2016-06-17 2022-08-31 Danisco US Inc. Protease variants and uses thereof
CN109563447A (zh) 2016-08-08 2019-04-02 巴斯夫欧洲公司 液体洗衣制剂
EP3535365A2 (en) 2016-11-07 2019-09-11 Danisco US Inc. Laundry detergent composition
MX2019006425A (es) 2016-12-01 2019-08-14 Basf Se Estabilizacion de enzimas en composiciones.
BR112019012258A2 (pt) * 2016-12-16 2020-10-20 Carbios proteases de degradação de plástico aprimoradas
CN110312795B (zh) 2016-12-21 2024-07-23 丹尼斯科美国公司 蛋白酶变体及其用途
EP3559226B1 (en) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus gibsonii-clade serine proteases
EP3601515A1 (en) 2017-03-31 2020-02-05 Danisco US Inc. Delayed release enzyme formulations for bleach-containing detergents
EP3601553B1 (en) 2017-03-31 2025-12-03 Danisco US Inc. Alpha-amylase combinatorial variants
CN110505803A (zh) 2017-04-13 2019-11-26 松下知识产权经营株式会社 水耕栽培装置
EP3404036A1 (en) * 2017-05-19 2018-11-21 Enzypep B.V. Subtilisin variants having a mutation in the s2 or s2' pocket
MX2019014556A (es) 2017-06-30 2020-02-07 Danisco Us Inc Particulas que contienen enzimas de baja aglomeracion.
EP3668973A2 (en) 2017-08-18 2020-06-24 Danisco US Inc. Alpha-amylase variants
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
CN111465680A (zh) 2017-11-29 2020-07-28 巴斯夫欧洲公司 组合物、其制备和用途
US20200354708A1 (en) * 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
MX2020006518A (es) 2017-12-21 2020-10-28 Danisco Us Inc Gránulos de fusión en caliente, que contienen enzimas, que comprenden un desecante termotolerante.
US20200359656A1 (en) 2018-02-08 2020-11-19 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
US11732250B2 (en) 2018-04-26 2023-08-22 Basf Se Lipase enzymes
WO2019211143A1 (en) 2018-05-03 2019-11-07 Basf Se Amylase enzymes
CN110607291B (zh) * 2018-06-14 2022-05-31 青岛蔚蓝生物集团有限公司 耐热木聚糖酶突变体
WO2019238761A1 (en) 2018-06-15 2019-12-19 Basf Se Water soluble multilayer films containing wash active chemicals and enzymes
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
CN108795906B (zh) * 2018-07-02 2022-02-22 四川德博尔制药有限公司 用于水解蛋壳膜的复合酶、蛋壳膜的水解方法、蛋壳膜水解产物及其提取方法
EP3830231A1 (en) 2018-07-31 2021-06-09 Danisco US Inc. Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid
WO2020030623A1 (en) 2018-08-10 2020-02-13 Basf Se Packaging unit comprising a detergent composition containing an enzyme and at least one chelating agent
WO2020047215A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Enzyme-containing granules
WO2020068486A1 (en) 2018-09-27 2020-04-02 Danisco Us Inc Compositions for medical instrument cleaning
US20210395651A1 (en) 2018-10-05 2021-12-23 Basf Se Compounds stabilizing hydrolases in liquids
EP3677676A1 (en) 2019-01-03 2020-07-08 Basf Se Compounds stabilizing amylases in liquids
CN112805377A (zh) 2018-10-05 2021-05-14 巴斯夫欧洲公司 在液体中稳定淀粉酶的化合物
BR112021005412A2 (pt) 2018-10-05 2021-06-15 Basf Se preparação de enzima, processo para fabricar uma preparação de enzima estável, métodos para reduzir a perda da atividade proteolítica, para preparação de uma formulação detergente, para remover manchas e para aumentar a estabilidade na armazenagem de uma formulação detergente líquida, usos de um composto e da preparação de enzima, e, formulação detergente
WO2020073553A1 (zh) * 2018-10-10 2020-04-16 上饶市康可得生物科技有限公司 筛选蛋白酶变体的方法以及获得的蛋白酶变体
BR112021006967A2 (pt) 2018-10-12 2021-07-13 Danisco Us Inc. alfa-amilases com mutações que melhoram a estabilidade na presença de quelantes
CN109337904B (zh) * 2018-11-02 2020-12-25 中国科学院动物研究所 基于C2c1核酸酶的基因组编辑系统和方法
WO2020104231A1 (en) 2018-11-19 2020-05-28 Basf Se Powders and granules containing a chelating agent and an enzyme
US12509672B2 (en) * 2018-11-28 2025-12-30 Danisco Us Inc. Subtilisin variants having improved stability
EP3891277A1 (en) * 2018-12-03 2021-10-13 Novozymes A/S Powder detergent compositions
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
CN114096676A (zh) 2019-02-20 2022-02-25 巴斯夫欧洲公司 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺
EP3702452A1 (en) * 2019-03-01 2020-09-02 Novozymes A/S Detergent compositions comprising two proteases
JP7209348B2 (ja) 2019-03-29 2023-01-20 パナソニックIpマネジメント株式会社 車両管理装置、車両管理方法及び車両
WO2020229480A1 (en) 2019-05-14 2020-11-19 Basf Se Compounds stabilizing hydrolases in liquids
US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
US20220306968A1 (en) 2019-06-06 2022-09-29 Danisco Us Inc Methods and compositions for cleaning
US12146171B2 (en) 2019-06-13 2024-11-19 Basf Se Method of recovering a protein from fermentation broth using a divalent cation
CN114008068A (zh) 2019-07-01 2022-02-01 巴斯夫欧洲公司 稳定酶的肽缩醛
US20220340865A1 (en) 2019-07-02 2022-10-27 Basf Se Method for preparing a fermentation medium
WO2021004830A1 (en) 2019-07-05 2021-01-14 Basf Se Industrial fermentation process for microbial cells using a fed-batch pre-culture
BR112022006082A2 (pt) 2019-10-18 2022-06-21 Basf Se Preparação enzimática, formulação de detergente, e, uso de pelo menos um diol
BR112022007697A2 (pt) 2019-10-24 2022-07-12 Danisco Us Inc Alfa-amilase variante que forma maltopentaose/maltohexaose
WO2021096857A1 (en) 2019-11-11 2021-05-20 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus cells
WO2021105336A1 (en) 2019-11-29 2021-06-03 Basf Se Compositions comprising polymer and enzyme
WO2021115912A1 (en) 2019-12-09 2021-06-17 Basf Se Formulations comprising a hydrophobically modified polyethyleneimine and one or more enzymes
CN115066495A (zh) 2020-02-14 2022-09-16 巴斯夫欧洲公司 甘露聚糖酶变体
PL4168523T3 (pl) 2020-06-18 2024-11-04 Basf Se Kompozycje i ich zastosowanie
EP4179053B1 (en) 2020-07-09 2024-04-03 Basf Se Compositions and their applications
WO2022008732A1 (en) 2020-07-10 2022-01-13 Basf Se Enhancing the activity of antimicrobial preservatives
EP4204553A1 (en) 2020-08-27 2023-07-05 Danisco US Inc. Enzymes and enzyme compositions for cleaning
WO2022043563A1 (en) * 2020-08-28 2022-03-03 Novozymes A/S Polyester degrading protease variants
WO2022083949A1 (en) 2020-10-20 2022-04-28 Basf Se Compositions and their use
WO2022106400A1 (en) * 2020-11-18 2022-05-27 Novozymes A/S Combination of immunochemically different proteases
CN112501149B (zh) * 2020-12-21 2022-03-18 天津科技大学 一种碱性蛋白酶突变体及其基因、工程菌、制备方法和应用
EP4284906A1 (en) 2021-01-29 2023-12-06 Danisco US Inc. Compositions for cleaning and methods related thereto
EP4363565A1 (en) 2021-06-30 2024-05-08 Danisco US Inc. Variant lipases and uses thereof
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
US20240384205A1 (en) 2021-09-03 2024-11-21 Danisco Us Inc. Laundry compositions for cleaning
CN117957318A (zh) 2021-09-13 2024-04-30 丹尼斯科美国公司 含有生物活性物质的颗粒
CN118202030A (zh) 2021-10-13 2024-06-14 巴斯夫欧洲公司 包含聚合物的组合物、聚合物及其用途
WO2023066741A1 (en) 2021-10-20 2023-04-27 Basf Se Phosphate-free composition and methods for their manufacture and use
MX2024006196A (es) 2021-11-22 2024-06-11 Basf Se Composiciones que comprenden polimeros, polimeros y su uso.
CN118451167A (zh) 2021-11-22 2024-08-06 巴斯夫欧洲公司 包含聚合物的组合物、聚合物及其用途
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
CN118679251A (zh) 2021-12-16 2024-09-20 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448747A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Variant maltopentaose/maltohexaose-forming alpha-amylases
WO2023110599A2 (en) 2021-12-17 2023-06-22 Basf Se Compositions and their applications
KR20240131442A (ko) 2022-01-13 2024-08-30 다니스코 유에스 인크. 그람 양성 박테리아 세포에서의 향상된 단백질 생산을 위한 조성물 및 방법
CN118974227A (zh) 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
CN119585409A (zh) 2022-07-15 2025-03-07 巴斯夫欧洲公司 用于液体配制品中酶稳定的烷醇胺甲酸盐
CN120112635A (zh) 2022-09-02 2025-06-06 丹尼斯科美国公司 枯草杆菌蛋白酶变体及其相关方法
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
EP4581119A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Detergent compositions and methods related thereto
WO2024083589A1 (en) 2022-10-18 2024-04-25 Basf Se Detergent compositions, polymers and methods of manufacturing the same
EP4615968A1 (en) 2022-11-09 2025-09-17 Danisco US Inc. Subtilisin variants and methods of use
EP4389864A1 (en) 2022-12-20 2024-06-26 Basf Se Cutinases
WO2024163600A1 (en) * 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
CN120712348A (zh) 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
EP4680013A1 (en) 2023-03-16 2026-01-21 Nutrition & Biosciences USA 4, Inc. Brevibacillus fermentate extracts for cleaning and malodor control and use thereof
CN121909283A (zh) 2023-09-28 2026-04-21 丹尼斯科美国公司 具有改善的溶解度的变体角质酶及其用途
CN121969733A (zh) 2023-10-20 2026-05-01 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2025132258A1 (en) 2023-12-20 2025-06-26 Basf Se Stabilized enzyme composition comprising a protease
EP4624572A1 (en) 2024-03-27 2025-10-01 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202379A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202369A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202370A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202374A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202382A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in cleaning compositions
WO2025202372A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
CN118703455B (zh) * 2024-07-02 2024-12-24 湖北大学 具有高催化活性的漆酶突变体及其制备方法和应用
WO2026024921A1 (en) 2024-07-25 2026-01-29 The Procter & Gamble Company Detergent composition comprising a subtilisin variant and methods of use
WO2026046881A1 (en) * 2024-08-26 2026-03-05 Novozymes A/S Compositions comprising a hexosaminidase and a protease
WO2026050315A1 (en) 2024-08-29 2026-03-05 Danisco Us Inc. Subtilisin variants and methods of use
CN118995679B (zh) * 2024-09-10 2025-04-29 华东师范大学 一种能够在纯水相环境中一步法催化肽键合成的枯草芽孢杆菌蛋白酶突变体及其应用

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
WO1995030010A1 (en) * 1994-05-02 1995-11-09 The Procter & Gamble Company Subtilisin bpn' variants having decreased adsorption and increased hydrolysis
WO1995029979A1 (en) * 1994-05-02 1995-11-09 The Procter & Gamble Company Fabric cleaning compositions containing subtilisin bpn' variants
US20020081703A1 (en) * 1998-04-15 2002-06-27 David A. Estell Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins
US20030118605A1 (en) * 2001-03-23 2003-06-26 Estell David A. Proteins producing an altered immunogenic response and methods of making and using the same
US6586223B1 (en) * 1999-07-22 2003-07-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US20040209343A1 (en) * 2003-01-30 2004-10-21 Novozymes A/S Novel subtilases
US20050054843A1 (en) * 2001-12-31 2005-03-10 Estell David A Proteases producing an altered immunological response and methods of making and using the same
US20050148059A1 (en) * 2001-12-31 2005-07-07 Estell David A. Protease producing an altered immunogenic response and methods of making and using the same
US20050239043A1 (en) * 2002-02-26 2005-10-27 Harding Fiona A Subtilisin carlsberg proteins with reduced immunogenicity

Family Cites Families (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1296839A (https=) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB2048606B (en) 1979-02-28 1983-03-16 Barr & Stroud Ltd Optical scanning system
US4302544A (en) 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GR76237B (https=) 1981-08-08 1984-08-04 Procter & Gamble
US4450235A (en) 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system
US4561998A (en) 1982-05-24 1985-12-31 The Procter & Gamble Company Near-neutral pH detergents containing anionic surfactant, cosurfactant and fatty acid
US4550862A (en) 1982-11-17 1985-11-05 The Procter & Gamble Company Liquid product pouring and measuring package with self draining feature
US4597898A (en) 1982-12-23 1986-07-01 The Proctor & Gamble Company Detergent compositions containing ethoxylated amines having clay soil removal/anti-redeposition properties
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4515707A (en) 1983-06-27 1985-05-07 The Chemithon Corporation Intermediate product for use in producing a detergent bar and method for producing same
DE3480411D1 (en) 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
US4515705A (en) 1983-11-14 1985-05-07 The Procter & Gamble Company Compositions containing odor purified proteolytic enzymes and perfumes
US4537706A (en) 1984-05-14 1985-08-27 The Procter & Gamble Company Liquid detergents containing boric acid to stabilize enzymes
US5972682A (en) 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5763257A (en) 1984-05-29 1998-06-09 Genencor International, Inc. Modified subtilisins having amino acid alterations
ES8706823A1 (es) 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
DK154572C (da) 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
DE3750450T2 (de) 1986-08-29 1995-01-05 Novo Industri As Enzymhaltiger Reinigungsmittelzusatz.
GB8629837D0 (en) 1986-12-13 1987-01-21 Interox Chemicals Ltd Bleach activation
US4765916A (en) 1987-03-24 1988-08-23 The Clorox Company Polymer film composition for rinse release of wash additives
US4972017A (en) 1987-03-24 1990-11-20 The Clorox Company Rinse soluble polymer film composition for wash additives
DE3851875T2 (de) 1987-05-29 1995-04-13 Genencor Int Cutinase haltige reinigungsmittelzusammensetzungen.
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DE68924654T2 (de) 1988-01-07 1996-04-04 Novonordisk As Spezifische Protease.
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
US4977252A (en) 1988-03-11 1990-12-11 National Starch And Chemical Investment Holding Corporation Modified starch emulsifier characterized by shelf stability
US4968451A (en) 1988-08-26 1990-11-06 The Procter & Gamble Company Soil release agents having allyl-derived sulfonated end caps
WO1990009446A1 (en) 1989-02-17 1990-08-23 Plant Genetic Systems N.V. Cutinase
DK0493398T3 (da) 1989-08-25 2000-05-22 Henkel Research Corp Alkalisk, proteolytisk enzym og fremgangsmåde til fremstilling deraf
KR100236540B1 (ko) 1990-04-14 2000-01-15 레클로우크스 라우에르 알카리성 바실러스-리파제, 이를 코-딩하는 dna 서열 및 리파제를 생산하는 바실러스 균주
US5354559A (en) 1990-05-29 1994-10-11 Grain Processing Corporation Encapsulation with starch hydrolyzate acid esters
ES2174820T3 (es) 1991-01-16 2002-11-16 Procter & Gamble Composiciones detergentes compactas con celulasa de alta actividad.
GB9108136D0 (en) 1991-04-17 1991-06-05 Unilever Plc Concentrated detergent powder compositions
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
FI952648A7 (fi) 1992-12-01 1995-07-28 Novozymes As Entsymaattisten reaktioiden tehostaminen
US5646101A (en) 1993-01-18 1997-07-08 The Procter & Gamble Company Machine dishwashing detergents containing an oxygen bleach and an anti-tarnishing mixture of a paraffin oil and sequestrant
CA2162459A1 (en) 1993-05-08 1994-11-24 Juergen Haerer Corrosion inhibitors for silver (ii)
US5888954A (en) 1993-05-08 1999-03-30 Henkel Kommanditgesellschaft Auf Aktien Corrosion inhibitors for silver
PL177936B1 (pl) 1993-05-08 2000-01-31 Henkel Kgaa Niskoalkaliczny środek do maszynowego zmywania naczyń
DK77393D0 (da) 1993-06-29 1993-06-29 Novo Nordisk As Aktivering af enzymer
US5698504A (en) 1993-07-01 1997-12-16 The Procter & Gamble Company Machine dishwashing composition containing oxygen bleach and paraffin oil and benzotriazole compound silver tarnishing inhibitors
US5486303A (en) 1993-08-27 1996-01-23 The Procter & Gamble Company Process for making high density detergent agglomerates using an anhydrous powder additive
DE4342680A1 (de) 1993-12-15 1995-06-22 Pfeiffer Erich Gmbh & Co Kg Austragvorrichtung für Medien
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US5691295A (en) 1995-01-17 1997-11-25 Cognis Gesellschaft Fuer Biotechnologie Mbh Detergent compositions
EP1921147B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
ES2364774T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
US5686014A (en) 1994-04-07 1997-11-11 The Procter & Gamble Company Bleach compositions comprising manganese-containing bleach catalysts
PE6995A1 (es) 1994-05-25 1995-03-20 Procter & Gamble Composicion que comprende un polimero de polialquilenoamina etoxilado propoxilado como agente de separacion de sucio
ES2180645T3 (es) 1994-06-17 2003-02-16 Genencor Int Metodo de limpieza basado en composiciones que contienen una enzima capaz de degradar las paredes celulares de las plantas y su uso en metodos de limpieza.
GB2294268A (en) 1994-07-07 1996-04-24 Procter & Gamble Bleaching composition for dishwasher use
US5879584A (en) 1994-09-10 1999-03-09 The Procter & Gamble Company Process for manufacturing aqueous compositions comprising peracids
US5691297A (en) 1994-09-20 1997-11-25 The Procter & Gamble Company Process for making a high density detergent composition by controlling agglomeration within a dispersion index
US5516448A (en) 1994-09-20 1996-05-14 The Procter & Gamble Company Process for making a high density detergent composition which includes selected recycle streams for improved agglomerate
US5489392A (en) 1994-09-20 1996-02-06 The Procter & Gamble Company Process for making a high density detergent composition in a single mixer/densifier with selected recycle streams for improved agglomerate properties
DK0796317T3 (da) 1994-12-09 2000-06-05 Procter & Gamble Diacylperoxidpartikelholdig sammensætning til automatisk opvask
US5534179A (en) 1995-02-03 1996-07-09 Procter & Gamble Detergent compositions comprising multiperacid-forming bleach activators
US5574005A (en) 1995-03-07 1996-11-12 The Procter & Gamble Company Process for producing detergent agglomerates from high active surfactant pastes having non-linear viscoelastic properties
US5569645A (en) 1995-04-24 1996-10-29 The Procter & Gamble Company Low dosage detergent composition containing optimum proportions of agglomerates and spray dried granules for improved flow properties
TR199701633T1 (xx) 1995-06-16 1998-04-21 The Procter & Gamble Company Kobalt kataliz�r i�eren otomatik bula��k makinas� deterjan bile�ikleri.
US5597936A (en) 1995-06-16 1997-01-28 The Procter & Gamble Company Method for manufacturing cobalt catalysts
US5565422A (en) 1995-06-23 1996-10-15 The Procter & Gamble Company Process for preparing a free-flowing particulate detergent composition having improved solubility
US5576282A (en) 1995-09-11 1996-11-19 The Procter & Gamble Company Color-safe bleach boosters, compositions and laundry methods employing same
EP0859828B2 (en) 1995-09-18 2006-06-14 The Procter & Gamble Company Delivery systems
MA24137A1 (fr) 1996-04-16 1997-12-31 Procter & Gamble Fabrication d'agents de surface ramifies .
US5929022A (en) 1996-08-01 1999-07-27 The Procter & Gamble Company Detergent compositions containing amine and specially selected perfumes
WO1998039405A1 (en) 1997-03-07 1998-09-11 The Procter & Gamble Company Bleach compositions containing metal bleach catalyst, and bleach activators and/or organic percarboxylic acids
CN1263759C (zh) 1997-03-07 2006-07-12 宝洁公司 制备交联桥大环化合物的改进方法
GB2327947A (en) 1997-08-02 1999-02-10 Procter & Gamble Detergent tablet
US6376445B1 (en) 1997-08-14 2002-04-23 Procter & Gamble Company Detergent compositions comprising a mannanase and a protease
DE19736591A1 (de) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
AR015977A1 (es) 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US5935826A (en) 1997-10-31 1999-08-10 National Starch And Chemical Investment Holding Corporation Glucoamylase converted starch derivatives and their use as emulsifying and encapsulating agents
US6287839B1 (en) 1997-11-19 2001-09-11 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
EP1032655B1 (en) 1997-11-21 2005-06-29 Novozymes A/S Protease variants and compositions
DE69837559T3 (de) 1997-12-24 2011-07-21 Genencor International, Inc., Calif. Verfahren zur Bestimmung der Waschleistung eines Enzyms.
BRPI9911086B1 (pt) 1998-06-10 2016-08-02 Novozymes As composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
US6294514B1 (en) 1998-11-24 2001-09-25 The Procter & Gamble Company Process for preparing mono-long chain amine oxide surfactants with low nitrite, nitrosamine and low residual peroxide
WO2000032601A2 (en) 1998-11-30 2000-06-08 The Procter & Gamble Company Process for preparing cross-bridged tetraaza macrocycles
US6440991B1 (en) 2000-10-02 2002-08-27 Wyeth Ethers of 7-desmethlrapamycin
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
GB0114847D0 (en) 2001-06-18 2001-08-08 Unilever Plc Water soluble package and liquid contents thereof
ES2336092T3 (es) * 2002-01-16 2010-04-08 Genencor International, Inc. Variantes de proteasas con multiples sustituciones.
US7557076B2 (en) 2002-06-06 2009-07-07 The Procter & Gamble Company Organic catalyst with enhanced enzyme compatibility
DE10260805A1 (de) 2002-12-23 2004-07-22 Geneart Gmbh Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins
ATE387487T1 (de) 2003-05-23 2008-03-15 Procter & Gamble Waschmittelzusammensetzung zum gebrauch in einer textilwasch- oder geschirrspülmaschine
ATE482972T1 (de) 2003-11-06 2010-10-15 Danisco Us Inc Tgf-beta1-bindende und geträgerte peptide
CN103333870A (zh) 2003-12-03 2013-10-02 丹尼斯科美国公司 过水解酶
US20080293610A1 (en) 2005-10-12 2008-11-27 Andrew Shaw Use and production of storage-stable neutral metalloprotease
WO2007145964A2 (en) 2006-06-05 2007-12-21 The Procter & Gamble Company Enzyme stabilizer
KR20090031906A (ko) 2006-07-18 2009-03-30 다니스코 유에스 인크. 광범위한 온도에 걸쳐 활성인 프로테아제 변이형
CA2690055A1 (en) * 2007-06-06 2008-12-18 Danisco Us Inc. Methods for improving protein performance
EP2100947A1 (en) 2008-03-14 2009-09-16 The Procter and Gamble Company Automatic dishwashing detergent composition
CN102046783A (zh) 2008-06-06 2011-05-04 丹尼斯科美国公司 包含变体微生物蛋白酶的组合物和方法
CN102652175B (zh) * 2009-12-09 2016-02-10 宝洁公司 织物和家居护理产品

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
WO1995030010A1 (en) * 1994-05-02 1995-11-09 The Procter & Gamble Company Subtilisin bpn' variants having decreased adsorption and increased hydrolysis
WO1995029979A1 (en) * 1994-05-02 1995-11-09 The Procter & Gamble Company Fabric cleaning compositions containing subtilisin bpn' variants
US20020081703A1 (en) * 1998-04-15 2002-06-27 David A. Estell Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins
US6586223B1 (en) * 1999-07-22 2003-07-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US20030118605A1 (en) * 2001-03-23 2003-06-26 Estell David A. Proteins producing an altered immunogenic response and methods of making and using the same
US20050054843A1 (en) * 2001-12-31 2005-03-10 Estell David A Proteases producing an altered immunological response and methods of making and using the same
US20050148059A1 (en) * 2001-12-31 2005-07-07 Estell David A. Protease producing an altered immunogenic response and methods of making and using the same
US20050239043A1 (en) * 2002-02-26 2005-10-27 Harding Fiona A Subtilisin carlsberg proteins with reduced immunogenicity
US20040209343A1 (en) * 2003-01-30 2004-10-21 Novozymes A/S Novel subtilases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PHILIP F. BRODE III ET AL.: ""Subtilisin BPN Variants: Increased Hydrolytic Activity on Surface-Bound Substrates via Decreased Surface Activity"", 《BIOCHEMISTRY》 *

Also Published As

Publication number Publication date
MX338884B (es) 2016-05-04
BR112012014082A2 (pt) 2017-05-23
US20200080069A1 (en) 2020-03-12
EP3190183A1 (en) 2017-07-12
MX356282B (es) 2018-05-22
US12203112B2 (en) 2025-01-21
EP3190183B1 (en) 2019-07-10
WO2011072099A2 (en) 2011-06-16
AR079338A1 (es) 2012-01-18
JP2017079756A (ja) 2017-05-18
CN102762222A (zh) 2012-10-31
MX2012006549A (es) 2012-08-23
BR112012014082B1 (pt) 2020-12-15
JP5863666B2 (ja) 2016-02-17
US20220204959A1 (en) 2022-06-30
DK3190183T3 (da) 2019-10-14
US20110262999A1 (en) 2011-10-27
US20160083710A1 (en) 2016-03-24
DK3599279T3 (da) 2026-03-30
DK2510094T3 (en) 2017-03-13
EP2510094B1 (en) 2016-11-30
CA2782891C (en) 2022-01-11
CA2782891A1 (en) 2011-06-16
EP2510094A4 (en) 2013-06-19
WO2011072099A3 (en) 2011-09-29
AU2010328121A1 (en) 2012-06-07
EP3599279A1 (en) 2020-01-29
JP6436957B2 (ja) 2018-12-12
JP2013513385A (ja) 2013-04-22
US20250327053A1 (en) 2025-10-23
US20140273177A1 (en) 2014-09-18
US9157052B2 (en) 2015-10-13
ES2746931T3 (es) 2020-03-09
EP2510094A2 (en) 2012-10-17
EP3599279B1 (en) 2026-02-18
CN102762222B (zh) 2015-11-25
JP2016028571A (ja) 2016-03-03
US8728790B2 (en) 2014-05-20
JP2019068807A (ja) 2019-05-09

Similar Documents

Publication Publication Date Title
US20240191220A1 (en) Compositions and methods comprising serine protease variants
CN102762222B (zh) 包含蛋白酶变体的组合物和方法
CN102858970B (zh) 包含变体蛋白酶的组合物和方法
AU2014206222B2 (en) Compositions and methods comprising protease variants

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160302