CN104770103B - A kind of method of oil Paeonia suffruticosa seed nursery - Google Patents
A kind of method of oil Paeonia suffruticosa seed nursery Download PDFInfo
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- CN104770103B CN104770103B CN201510178327.2A CN201510178327A CN104770103B CN 104770103 B CN104770103 B CN 104770103B CN 201510178327 A CN201510178327 A CN 201510178327A CN 104770103 B CN104770103 B CN 104770103B
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Abstract
The invention discloses a kind of method of oil Paeonia suffruticosa seed nursery, including step: (1) chooses seeds；(2) seed soaking sterilization；(3) cold plasma utilizing atmospheric dielectric barrier discharge to generate processes the oil Paeonia suffruticosa seed of step (2), and plasma treatment seed time is 50 60s；(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L；(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after processing step (3) is mixed in 1:3 5:1 2:1 2 ratio with fine sand, perlite, vermiculite, adjustment humidity, to 40 50%, carries out alternating temperature stratification process by three phases in constant incubator；(6) sow；(7) seedling management.The oil of the present invention method of Paeonia suffruticosa seed nursery, in cultivating process, can effectively abolish seed dormancy, make seed germinate rapidly, accelerate seed germination, improve seeding ratio, shorten seedling-growing time, emerge neat, and improve the growth indexes such as the fresh weight of seedling, plant height, root system, environmentally safe to some extent.
Description
Technical field
The present invention relates to flower seed seedling-raising technique field, particularly relate to the side of a kind of oil Paeonia suffruticosa seed nursery
Method.
Background technology
Oil is the one of national flower tree peony with tree peony, is the plant that traditional oil tree has again medical value,
Also it is the high green tree species of ornamental value, strong adaptability, drought-resistant barren, it not only is suitable in hilly country but also fit
Being combined in the growth of the north of the Changjiang River, Anhui plains region, being to implement soil synergy, greening is rural, improve the excellent of ecological condition
Seeds.Oil its root skin of tree peony has analgesia, refrigerant antipyretic, anti-inflammation, immunity etc. medicinal, element and the root of herbaceous peony,
Chrysanthemum, Poria cocos the referred to as big famous medicine in Anhui four, be also one of 34 kinds of rare medicinal herbs in the whole nation.Peony seed oil for
Prevention and the treatment common cardiovascular diseases such as hypertension, high fat of blood have certain curative effect, also have diabetic aobvious
The food therapy effect writing, and without any bad reaction.At present oil often have aborning with tree peony Ye Wuhua or
Having Hua Wuye, the phenomenon even do not sprouted, this is primarily due to oil and is not released from dormancy or dormancy with tree peony bud
Releasing insufficient causing, oil has the characteristic of top-wall effect with tree peony, after autumn sowing, radicle growth become root and
Epicotyl does not extends, in a dormant state, it is necessary to after low temperature after a while, epicotyl could be sprouted.Separately
Outward, current oil is low with tree peony germination percentage, and most seeds lose vigor in long sowing and storage,
Loss of seeds is serious.
Content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, a kind of method of oil Paeonia suffruticosa seed nursery be provided,
In cultivating process, can effectively abolish seed dormancy, make seed germinate rapidly, accelerate seed germination, carry
High seeding ratio, shortens seedling-growing time, emerges neat, and improve to some extent the fresh weight of seedling, plant height,
The growth indexes such as root system, environmentally safe.
The present invention adopts the following technical scheme that to achieve these goals
A kind of method of oil Paeonia suffruticosa seed nursery, comprises the following steps:
(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, with clear water floatation go the removal of impurity and
Flat grain, chooses healthy oil Paeonia suffruticosa seed, and oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%；
(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours,
Further take out and be filtered dry the surface of the seed moisture, then with 10% hypochlorite disinfectant's 10-15 minute, aseptic water washing
3-5 time, standby after the surface of the seed moisture that dries in the shade；
(3) tree peony used by the oil that the cold plasma utilizing atmospheric dielectric barrier discharge to generate processes step (2)
Seed, the time of plasma treatment seed is 50-60s；
(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L；
(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) is processed and fine sand, perlite, vermiculite
In the ratio mixing of 1:3-5:1-2:1-2, adjustment humidity is to 40-50%, according to such as in constant incubator
Under carry out the alternating temperature stratification process of three phases:
In the stage one: lamination 5-8 days under the conditions of 10-20 DEG C, period is constantly stirred and sprays step (4) and joins
The treatment fluid of system, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage two: lamination 15-20 days under the conditions of 3-6 DEG C, period is constantly stirred and sprays step (4) and joins
The treatment fluid of system, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage three: lamination 5-8 days under the conditions of-2-3 DEG C, period is constantly stirred and sprays step (4) preparation
Treatment fluid, be limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, every day spray once, obtain sprout kind
Son；
(6) sow: use drilling method to be sown into the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order,
Cover thin soil after broadcasting and hide seed, then cover with withered grass, cultivate seed sprouting；
(7) seedling management: topdress in seedling stage, exposes bud at oil tree peony flower bud development to leaflet petiole
During squama, outside scale and bottom 2-3 piece leaflet are peeled off.
The power supply of the dielectric barrier discharge described in step (3) is high-frequency and high-voltage power supply, and frequency is 3-5kHz,
Voltage is 5-7kV.
Compared with the prior art, beneficial effects of the present invention is as follows:
(1) oil of the present invention method of Paeonia suffruticosa seed nursery, in cultivating process, comprehensive utilization growth is adjusted
The joint technology such as agent and plasma treatment breaks seed dormancy, makes seed germinate rapidly, accelerates seed germination,
Improve seeding ratio, shorten seedling-growing time, emerge neat, and improve the fresh weight of seedling, strain to some extent
The growth indexes such as height, root system, environmentally safe；
(2) present invention is chosen seeds by application clear water floatation and ensure that seed quality and vigor, water seed soaking,
Make the abundant imbibition of seed；Hypochlorite disinfectant, prevents seed from going mouldy；Comprehensive utilization growth regulator and etc. from
Daughter process, relieves oil Paeonia suffruticosa seed dormancy effectively；Utilize the performance of gibberellin, break seed dormancy
Phase, promote to germinate ahead of time, and improve germination percentage；Utilize the performance of heteroauxin, promote that oil tree peony seedling is raw
Root, improves the yield of moutan root bark.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but the present invention is not limited only to these embodiments,
On the premise of without departing from present inventive concept, any improvement made all falls within protection scope of the present invention.
Embodiment 1:
A kind of method of oil Paeonia suffruticosa seed nursery, comprises the following steps:
(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, with clear water floatation go the removal of impurity and
Flat grain, chooses healthy oil Paeonia suffruticosa seed, and oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%；
(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours,
Further take out and be filtered dry the surface of the seed moisture, then with 10% hypochlorite disinfectant 10 minutes, aseptic water washing 5
Secondary, standby after the surface of the seed moisture that dries in the shade；
(3) tree peony used by the oil that the cold plasma utilizing atmospheric dielectric barrier discharge to generate processes step (2)
Seed, the time of plasma treatment seed is 50s；The power supply of described dielectric barrier discharge is high-frequency and high-voltage
Power supply, frequency is 3kHz, and voltage is 5kV.
(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L；
(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) is processed and fine sand, perlite, vermiculite
In the ratio mixing of 1:3:2:2, adjustment humidity, to 40%, proceeds as follows three in constant incubator
The alternating temperature stratification process in stage:
In the stage one: lamination 8 days under the conditions of 10 DEG C, the place that step (4) is prepared constantly is stirred and sprayed to period
Reason liquid, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage two: lamination 20 days under the conditions of 3 DEG C, the place that step (4) is prepared constantly is stirred and sprayed to period
Reason liquid, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage three: lamination 8 days under the conditions of-2 DEG C, the place that step (4) is prepared constantly is stirred and sprayed to period
Reason liquid, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once, obtains the seed sprouted；
(6) sow: use drilling method to be sown into the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order,
Cover thin soil after broadcasting and hide seed, then cover with withered grass, cultivate seed sprouting；
(7) seedling management: topdress in seedling stage, exposes bud at oil tree peony flower bud development to leaflet petiole
During squama, outside scale and 2, bottom leaflet are peeled off.
Embodiment 2:
A kind of method of oil Paeonia suffruticosa seed nursery, comprises the following steps:
(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, with clear water floatation go the removal of impurity and
Flat grain, chooses healthy oil Paeonia suffruticosa seed, and oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%；
(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours,
Further take out and be filtered dry the surface of the seed moisture, then with 10% hypochlorite disinfectant 15 minutes, aseptic water washing 3-5
Secondary, standby after the surface of the seed moisture that dries in the shade；
(3) tree peony used by the oil that the cold plasma utilizing atmospheric dielectric barrier discharge to generate processes step (2)
Seed, the time of plasma treatment seed is 60s；The power supply of described dielectric barrier discharge is high-frequency and high-voltage
Power supply, frequency is 5kHz, and voltage is 7kV.
(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L；
(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) is processed and fine sand, perlite, vermiculite
In the ratio mixing of 1:5:1:1, adjustment humidity, to 50%, proceeds as follows three in constant incubator
The alternating temperature stratification process in stage:
In the stage one: lamination 5 days under the conditions of 20 DEG C, the place that step (4) is prepared constantly is stirred and sprayed to period
Reason liquid, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage two: lamination 15 days under the conditions of 6 DEG C, the place that step (4) is prepared constantly is stirred and sprayed to period
Reason liquid, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage three: lamination 5 days under the conditions of-3 DEG C, the place that step (4) is prepared constantly is stirred and sprayed to period
Reason liquid, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once, obtains the seed sprouted；
(6) sow: use drilling method to be sown into the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order,
Cover thin soil after broadcasting and hide seed, then cover with withered grass, cultivate seed sprouting；
(7) seedling management: topdress in seedling stage, exposes bud at oil tree peony flower bud development to leaflet petiole
During squama, outside scale and 3, bottom leaflet are peeled off.
Claims (2)
1. the oil method of Paeonia suffruticosa seed nursery, it is characterised in that: comprise the following steps:
(1) choose seeds: select ripe, disease-free oil Paeonia suffruticosa seed, with clear water floatation go the removal of impurity and
Flat grain, chooses healthy oil Paeonia suffruticosa seed, and oil Paeonia suffruticosa seed cleanliness is 100%, seed vitality more than 95%；
(2) seed soaking sterilization: the oil Paeonia suffruticosa seed of step (1) is placed in 40 DEG C of water seed soaking 24 hours,
Further take out and be filtered dry the surface of the seed moisture, then with 10% hypochlorite disinfectant's 10-15 minute, aseptic water washing
3-5 time, standby after the surface of the seed moisture that dries in the shade；
(3) tree peony used by the oil that the cold plasma utilizing atmospheric dielectric barrier discharge to generate processes step (2)
Seed, the time of plasma treatment seed is 50-60s；
(4) treatment fluid is prepared: gibberellin 0.25mg/L, heteroauxin 2mg/L, calcium chloride 3mg/L；
(5) alternating temperature stratification: the oil Paeonia suffruticosa seed after step (3) is processed and fine sand, perlite, vermiculite
In the ratio mixing of 1:3-5:1-2:1-2, adjustment humidity is to 40-50%, according to such as in constant incubator
Under carry out the alternating temperature stratification process of three phases:
In the stage one: lamination 5-8 days under the conditions of 10-20 DEG C, period is constantly stirred and sprays step (4) and joins
The treatment fluid of system, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage two: lamination 15-20 days under the conditions of 3-6 DEG C, period is constantly stirred and sprays step (4) and joins
The treatment fluid of system, is limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, and every day sprays once；
In the stage three: lamination 5-8 days under the conditions of-2-3 DEG C, period is constantly stirred and sprays step (4) preparation
Treatment fluid, be limited with fine sand, perlite, vermiculite with Paeonia suffruticosa seed, every day spray once, obtain sprout kind
Son；
(6) sow: use drilling method to be sown into the oil Paeonia suffruticosa seed of sprouting in the sand soil put in order,
Cover thin soil after broadcasting and hide seed, then cover with withered grass, cultivate seed sprouting；
(7) seedling management: topdress in seedling stage, exposes bud at oil tree peony flower bud development to leaflet petiole
During squama, outside scale and bottom 2-3 piece leaflet are peeled off.
2. the method for oil Paeonia suffruticosa seed nursery according to claim 1, it is characterised in that: step (3)
Described in the power supply of dielectric barrier discharge be high-frequency and high-voltage power supply, frequency is 3-5kHz, and voltage is 5-7kV.
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