CN103299743A - Method for breaking sweet-scented osmanthus seed dormancy - Google Patents
Method for breaking sweet-scented osmanthus seed dormancy Download PDFInfo
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Abstract
The invention discloses a method for breaking sweet-scented osmanthus seed dormancy. The method comprises the following steps of seed selection, seed soaking treatment, plasma treatment, comprehensive treatment by gibberellin and hydrogen peroxide, low-temperature stratifcation treatment and the like. According to the method disclosed by the invention, proper frequency, voltage and treatment time of the plasma treatment are selected so that the later low-temperature treatment time is shortened. As the gibberellin and the hydrogen peroxide are applied to the comprehensive treatment of the seed, a substance inhibiting seed germination is effectively broken, and the time for breaking the dormancy is shortened.
Description
Technical field
What the present invention relates to is a kind of method of breaking fast the fragrans seed dormancy.
Background technology
Sweet osmanthus is one of China's ten great tradition famous flowers, also is one of most precious flowers and trees of modern city greening.Its tree performance is graceful, flourishing, greenery are verdant, evergreen all the year round, especially tempting with the deep and remote fragrance of a flower that disappears, really be " monopolize three autumn jobs as harvesting, tilling and planting press many virtue ", be decided to be city flower by world-renowned tourist cities such as Suzhou, Hangzhou, Guilin.The demand of sweet osmanthus is increasing now, and the Fast-propagation of sweet osmanthus is a problem that can not be ignored.
Seed dormancy is the same with many woody plants with low temperature depositing, and fragrans seed also has one period long resting stage.The fragrans seed of now gathering is not sprouted then basically, only has through just germinateing spring in Second Year behind the winter dormancy.No matter seed sows in the ground still indoor seasoning in very long like this some months resting stage, all may and go mouldy because of insect pest, the plague of rats, suffers unnecessary loss.And the sweet osmanthus sowing time can be again about the October in autumn, yet the fragrans seed of gathering does not then also have breaking dormancy, can not sprout and grow seedlings.The method of conventional breaking dormancy is that the fragrans seed through the mulling storage is carried out vernalization through wet husky the processing, this method length that expends time in, and germination rate is low; Also have research and utilization gibberellin to soak again and come breaking dormancy through cold stratification, but still exist germination rate low, the problem that the time is relatively long is so it is very necessary further to study a kind of method of breaking fast the fragrans seed dormancy.
Summary of the invention
The present invention is directed to existing deficiency a kind of method of breaking the fragrans seed dormancy is provided.
The present invention realizes above-mentioned purpose by the following method.
A kind of method of breaking the fragrans seed dormancy, the method comprises the following steps:
(1) seed selection: during 4 ~ May, when the pericarp of fragrans seed changes hyacinthine into gradually by green, can gather.The rear watering stack retting of gathering is removed pulp, the seed that dries in the shade, mulling storage.Choose the full seed of results, dry at the moon place that ventilates;
(2) seed of choosing is cleaned with clear water first, the liquor potassic permanganate of then putting into 0.5 ~ 1% concentration soaked 1 ~ 2 hour, dried after cleaning with clear water;
(3) cold plasma that utilizes atmospheric dielectric barrier discharge to generate is processed the fragrans seed that obtains through step (2), the time of plasma treatment seed is 60 ~ 80 seconds, the power supply of described dielectric barrier discharge is high-frequency and high-voltage power supply, frequency range is 4kHz ~ 6kHz, and voltage range is 5kV ~ 7kV;
(4) with the fragrans seed in the step (3), be that 0.04 ~ 0.6% gibberellin was processed 1 ~ 2 day with mass fraction, be that 10 ~ 15% hydrogen peroxide was processed 1 ~ 2 day with mass fraction again, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2 ~ 4 ℃ carried out cold stratification 40 ~ 60 days.
Beneficial effect:
(1) technology of plasma treatment of the present invention comprises the time of having adopted suitable frequency, voltage and processing, so that later stage low temperature treatment time shorten.
(2) utilize gibberellin and hydrogen peroxide that seed is carried out integrated treatment, abolished effectively the material that suppresses seed germination, shortened the time of breaking dormancy.
Embodiment
The present invention is further illustrated below in conjunction with embodiment, and following each embodiment only is used for explanation the present invention, to not restriction of the present invention.
Embodiment one
A kind of method of breaking the fragrans seed dormancy, the method comprises the following steps:
(1) seed selection: during 4 ~ May, when the pericarp of fragrans seed changes hyacinthine into gradually by green, can gather.The rear watering stack retting of gathering is removed pulp, the seed that dries in the shade, mulling storage.Choose the full seed of results, dry at the moon place that ventilates;
(2) seed of choosing is cleaned with clear water first, the liquor potassic permanganate of then putting into 0.5% concentration soaked 1 hour, dried after cleaning with clear water;
(3) cold plasma that utilizes atmospheric dielectric barrier discharge to generate is processed the fragrans seed that obtains through step (2), the time of plasma treatment seed is 60 seconds, the power supply of described dielectric barrier discharge is high-frequency and high-voltage power supply, and frequency range is 4kHz, and voltage range is 5kV;
(4) with the fragrans seed in the step (3), be that 0.04% gibberellin was processed 1 day with mass fraction, be that 15% hydrogen peroxide was processed 2 days with mass fraction again, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2 ~ 4 ℃ carried out cold stratification 40 days.
Percentage of seedgermination after the above-mentioned processing of process is 85%.
Embodiment two
A kind of method of breaking the fragrans seed dormancy, the method comprises the following steps:
(1) seed selection: during 4 ~ May, when the pericarp of fragrans seed changes hyacinthine into gradually by green, can gather.The rear watering stack retting of gathering is removed pulp, the seed that dries in the shade, mulling storage.Choose the full seed of results, dry at the moon place that ventilates;
(2) seed of choosing is cleaned with clear water first, the liquor potassic permanganate of then putting into 1% concentration soaked 2 hours, dried after cleaning with clear water;
(3) cold plasma that utilizes atmospheric dielectric barrier discharge to generate is processed the fragrans seed that obtains through step (2), the time of plasma treatment seed is 80 seconds, the power supply of described dielectric barrier discharge is high-frequency and high-voltage power supply, and frequency range is 4kHz, and voltage range is 7kV;
(4) with the fragrans seed in the step (3), be that 0.06% gibberellin was processed 2 days with mass fraction, be that 10% hydrogen peroxide was processed 1 day with mass fraction again, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2 ~ 4 ℃ carried out cold stratification 60 days.
Fragrans seed germination rate after the above-mentioned processing of process is 90%
Embodiment three
A kind of method of breaking the fragrans seed dormancy, the method comprises the following steps:
(1) seed selection: during 4 ~ May, when the pericarp of fragrans seed changes hyacinthine into gradually by green, can gather.The rear watering stack retting of gathering is removed pulp, the seed that dries in the shade, mulling storage.Choose the full seed of results, dry at the moon place that ventilates;
(2) seed of choosing is cleaned with clear water first, the liquor potassic permanganate of then putting into 1% concentration soaked 1.5 hours, dried after cleaning with clear water;
(3) cold plasma that utilizes atmospheric dielectric barrier discharge to generate is processed the fragrans seed that obtains through step (2), the time of plasma treatment seed is 70 seconds, the power supply of described dielectric barrier discharge is high-frequency and high-voltage power supply, and frequency range is 5kHz, and voltage range is 6kV;
(4) with the fragrans seed in the step (3), be that 0.05% gibberellin was processed 1.5 days with mass fraction, be that 12% hydrogen peroxide was processed 1.5 days with mass fraction again, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2 ~ 4 ℃ carried out cold stratification 50 days.
The germination rate of the fragrans seed of the above-mentioned processing of process is 98%.
Claims (1)
1. a method of breaking the fragrans seed dormancy is characterized in that the method comprises the following steps:
(1) seed selection: during 4 ~ May, can gather when the pericarp of fragrans seed changes hyacinthine into gradually by green, the rear watering stack retting of gathering is removed pulp, the seed that dries in the shade, and full seed is chosen in the mulling storage, dries at the moon place that ventilates;
(2) seed of choosing is cleaned with clear water first, the liquor potassic permanganate of then putting into 0.5 ~ 1% concentration soaked 1 ~ 2 hour, dried after cleaning with clear water;
(3) cold plasma that utilizes atmospheric dielectric barrier discharge to generate is processed the fragrans seed that obtains through step (2), the time of plasma treatment seed is 60 ~ 80 seconds, the power supply of described dielectric barrier discharge is high-frequency and high-voltage power supply, frequency range is 4kHz ~ 6kHz, and voltage range is 5kV ~ 7kV;
(4) with the fragrans seed in the step (3), be that 0.04 ~ 0.06% gibberellin was processed 1 ~ 2 day with mass fraction, be that 10 ~ 15% hydrogen peroxide was processed 1 ~ 2 day with mass fraction again, then put into cleaned container with the wet husky ratio according to 1:4 of preprepared through mixing, cover with preservative film, and prick the breathing that several apertures are beneficial to seed at preservative film, the refrigerator of putting into 2 ~ 4 ℃ carried out cold stratification 40 ~ 60 days.
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103444396A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Cultivation for promoting seed germination of cercidiphyllum japonicum and increasing seed germination rate |
CN104067919A (en) * | 2014-07-03 | 2014-10-01 | 李永青 | Ailanthus altissima seedling breeding method |
CN104620721A (en) * | 2015-03-13 | 2015-05-20 | 西南林业大学 | Method for promoting germination of sweet osmanthus seeds |
CN104770103A (en) * | 2015-04-15 | 2015-07-15 | 芜湖市丹洋现代农业科技发展有限公司 | Seedling raising method for oil-used peony seeds |
CN105284531A (en) * | 2015-10-30 | 2016-02-03 | 广西金神农生态农业科技开发有限公司 | "Or" seedling cultivation method |
CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
CN105993279A (en) * | 2016-05-18 | 2016-10-12 | 合肥公盈园艺科技有限公司 | Method for cultivating seedlings |
CN106342624A (en) * | 2016-08-23 | 2017-01-25 | 贵州玉龙泽洋种养殖有限公司 | Landscape sweet osmanthus seedling raising method |
CN107371460A (en) * | 2017-08-30 | 2017-11-24 | 马鞍山市永辉园林绿化有限公司 | A kind of processing method of fragrans seed |
CN111295971A (en) * | 2020-03-21 | 2020-06-19 | 郑州市农林科学研究所 | Method for rapidly breaking dormancy of osmanthus fragrans seeds |
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JP2007202481A (en) * | 2006-02-02 | 2007-08-16 | Nagasaki Prefecture | Fermented tea |
CN102598918A (en) * | 2012-03-30 | 2012-07-25 | 常熟市新靓文辅亚农艺发展有限公司 | Method for culturing osmanthus seedlings |
CN102919035A (en) * | 2012-11-19 | 2013-02-13 | 罗家豪 | Industrialized cultivation method of sweet-scented osmanthus nursery stock |
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JP2005211818A (en) * | 2004-01-30 | 2005-08-11 | Mitsubishi Chemicals Corp | Adsorbent |
JP2007202481A (en) * | 2006-02-02 | 2007-08-16 | Nagasaki Prefecture | Fermented tea |
CN102598918A (en) * | 2012-03-30 | 2012-07-25 | 常熟市新靓文辅亚农艺发展有限公司 | Method for culturing osmanthus seedlings |
CN102919035A (en) * | 2012-11-19 | 2013-02-13 | 罗家豪 | Industrialized cultivation method of sweet-scented osmanthus nursery stock |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103444396A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Cultivation for promoting seed germination of cercidiphyllum japonicum and increasing seed germination rate |
CN104067919A (en) * | 2014-07-03 | 2014-10-01 | 李永青 | Ailanthus altissima seedling breeding method |
CN104067919B (en) * | 2014-07-03 | 2016-03-09 | 李永青 | The method of tree-of-heaven nursery |
CN104620721A (en) * | 2015-03-13 | 2015-05-20 | 西南林业大学 | Method for promoting germination of sweet osmanthus seeds |
CN104620721B (en) * | 2015-03-13 | 2017-03-01 | 西南林业大学 | A kind of method promoting fragrans seed to sprout |
CN104770103B (en) * | 2015-04-15 | 2016-11-02 | 芜湖市丹洋现代农业科技发展有限公司 | A kind of method of oil Paeonia suffruticosa seed nursery |
CN104770103A (en) * | 2015-04-15 | 2015-07-15 | 芜湖市丹洋现代农业科技发展有限公司 | Seedling raising method for oil-used peony seeds |
CN105284531A (en) * | 2015-10-30 | 2016-02-03 | 广西金神农生态农业科技开发有限公司 | "Or" seedling cultivation method |
CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
CN105684595B (en) * | 2016-01-19 | 2018-11-13 | 河北省林业科学研究院 | There is the method for whisker iris hybrid seed breaking dormancy |
CN105993279A (en) * | 2016-05-18 | 2016-10-12 | 合肥公盈园艺科技有限公司 | Method for cultivating seedlings |
CN106342624A (en) * | 2016-08-23 | 2017-01-25 | 贵州玉龙泽洋种养殖有限公司 | Landscape sweet osmanthus seedling raising method |
CN107371460A (en) * | 2017-08-30 | 2017-11-24 | 马鞍山市永辉园林绿化有限公司 | A kind of processing method of fragrans seed |
CN111295971A (en) * | 2020-03-21 | 2020-06-19 | 郑州市农林科学研究所 | Method for rapidly breaking dormancy of osmanthus fragrans seeds |
CN111295971B (en) * | 2020-03-21 | 2021-11-26 | 郑州市农林科学研究所 | Method for rapidly breaking dormancy of osmanthus fragrans seeds |
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Application publication date: 20130918 |