CN105409378A - Method for promoting germination of tree seeds - Google Patents

Method for promoting germination of tree seeds Download PDF

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CN105409378A
CN105409378A CN201510696396.2A CN201510696396A CN105409378A CN 105409378 A CN105409378 A CN 105409378A CN 201510696396 A CN201510696396 A CN 201510696396A CN 105409378 A CN105409378 A CN 105409378A
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seed
blue
soaking
germination
green money
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CN105409378B (en
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王春爱
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Shandong Shuntong Environment Protection Material Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The present invention discloses a method for promoting germination of tree seeds, and belongs to the field of tree proliferation. The method comprises the following steps of: (1) seed soaking treatment: soaking seeds for 9 h by using seed soaking liquid at the temperature of 30 DEG C; wherein a preparation method of the soaking liquid with the following components comprises: adding 0.6 g of NaCl, 0.98 g of KCl, 0.17 g of CaCl2.2H2O, 0.11 g of MgCl2.6H2O, 30 mg of methylacrylic acid alkyl alcohol polyoxyethylene ester, 40 mg of gamma-aminobutyric acid, and 0.2 g of asta-xanthin into 1000 ml of distilled water to obtain a suspension; and (2) germination treatment: covering the cyclocarya paliurus seed obtained in the step (1) with straws, insulating and germinating, keeping the temperature at 20 DEG C and humidity at 60%, and storing for eight days. Compared with the prior art, the method for promoting germination of tree seeds can promote germination of the cyclocarya paliurus seeds, so that a higher germination rate is obtained, and a higher seedling survival rate can be obtained at a relatively low temperature.

Description

A kind of trees urge kind of the method sprouted
Technical field
The present invention is application for a patent for invention " a kind of seed soaking agent of short blue or green money willow seed germination " (application number: divisional application 2013104708274), relate to a kind of seed soaking agent, particularly trees urge kind of the method sprouted, and may be used for low temperature environment and cultivate, and the sprouting of new seed.
Background technology
Blue or green money willow (CyclocaryapaliurusIljin.) is exclusive autogenus magaphanerophytes of China, is one of endangered plants of special-protection-by-the-State, belongs to three class protective plants.Blue or green money willow kind is of many uses, is a kind of rare tree having very much growth and development Utilization prospects.Research finds not only containing macroelements such as the necessary potassium of human body, calcium, magnesium, phosphorus in Qingqian Willow leaf, but also containing the trace element played an important role to body-care as manganese, iron, copper, zinc, selenium, chromium, alum, germanium etc.; Blue or green money willow glucoside I (cyclocariosideI) of extracting from Qingqian Willow leaf sheet is one of 40 kinds of native compounds with sweet taste found in the world at present, has very important research using value; Blue or green money willow tender leaf can for tea, containing polysaccharide compound, flavonoids, oleanolic acid, frankincense, terpenoid in leaf, in addition containing organic acid, alkaloid, and sterol is much to the favourable inorganic substances of human body and organic substance, has multiple medicine and health care function.
Blue or green money willow has high protection and development and application values.But existing blue or green money willow resource mainly natural forest, not only quantity is few, and how to be sporadicly distributed in remote, thickly forested mountains and some nature reserves.Blue or green money willow seed has deep dormancy habit, and naturally more raw rate is low, and artificial cuttage, to cultivate blue or green money willow survival rate not high yet, had a strong impact on exploitation and the industrialization process of blue or green money willow.At present, there is no the successful experience of a large amount of artificial propagation and the blue or green money willow of cultivation both at home and abroad, the artificial propagation techniques therefore studying blue or green money willow becomes the task of top priority developing blue or green money willow.
At present sexual reproduction and vegetative propagation are mainly included to the exploration of blue or green money willow propagation technique research, but be still in the exploratory stage to the Study on tissue culture of blue or green money willow both at home and abroad, relevant research only obtains callus but fails the formation of induced bud, above generally still uses seed natural propagation so produce.
Blue or green money willow fruit is nut, and common name seed, in oblate spheroid, shape is as copper coin.The kind shell of blue or green money willow seed is hard, and generally after planting need within 1 ~ 3 year, sprout, natural germination rate is low, is only 0.1 ~ 0.2%.Research shows that blue or green money willow seed has deep dormancy characteristic.Previously research shows the seed dormant factor variation of blue or green money willow, belong to comprehensive deep dormancy type: rigid shell causes its main cause of forcing dormancy, it had both fettered embryo, have impact on gas permeability again, but still there is water penetration, secondly, seed also also exists physiological dormancy, and seed coat hinders embryo and extraneous water_air exchange to a certain extent; Meanwhile, in seed coat, have accumulated a large amount of inhibiting substances, make rataria penetration depth resting state.Therefore, in sexual propagation, Seed germination techniques is the Focal point and difficult point of research always.Numerous scholar was studied it in recent years, and people bury with except the vernalization such as emerge in worm water except carrying out wet sand to seed, also adopt cool stratification, acid etching and gibberellin process to seed, to improve the germination rate of seed, shorten growing-seedling period.As adopted Exogenous gibberellic acid process seed, can not only seeding ratio be improved, effectively can also shorten growing-seedling period.But still there is following defect in such scheme: 1. GA 3(gibberellin) is a most widely used plant growth regulators on the current agriculture of China, woods, gardening, be mainly used in promoting growing of crop, premature ripening, improve output and the dormancy of breaking the organs such as seed, stem tuber, bulb, but the Main Function of gibberellin promotes cell elongation, thus cause cane elongation and plant to increase, at use GA 3if the unfavourable weather conditions such as rear drought, due to GA 3the growth of overrunning brought, then can increase the weight of the injury of seedling, cause death of seedling, and above reason limits the application at blue or green money willow seed, is only limited to laboratory research field; 2. still there is seed germination time disunity, percentage of seedgermination is difficult to problems such as effectively increasing substantially, limit blue or green money willow genetic breeding and production application; 3. blue or green money willow is mainly distributed in the ground such as Anhui, Jiangsu, Zhejiang, Jiangxi, Fujian, and in Shandong there are no growth, its reason also more is germination period and Seedling Stage, and seed germination and growth of seedling are the tricky time to adverse circumstance in early days, and resistance is lower.Under natural conditions, it is higher that blue or green money willow sprouts environmental requirement, and tolerance to cold is general, happiness warm and moist weather.In prior art: sprouting is 2 ~ mid-March, temperature is 14.0 ~ 20.0 DEG C, then at the beginning of late May to 6 month, and final singling during seedling 10 ~ 20cm height.But the temperature of Shandong District was then 5 ~ 14 DEG C, well below the Yangtze river basin same period.If if but yield to sprouting nursery temperature, delay and sow to during proper temperature mid-April, then when seedling height of seedling does not also reach 10cm, now enter summer, high temperature is hot, and with the dry season, air humidity is lower, simultaneously subterranean water level is high, soil drainage poor-performing, it's the summer easily causes cannot past seedling and causes death, even if adopt fluffy shade process, nursery stock comes through, but poor growth, even stagnate.
Therefore, remove blue or green money willow seed dormancy and promote that its sprouting has definite meaning to blue or green money willow germ plasm resource preservation and utilization, the industry development of blue or green money willow.
Summary of the invention
Technical assignment of the present invention is for the deep dormancy of blue or green money willow seed, the characteristic slow, emergence rate is low of germinateing, and provides a kind of special seed soaking agent being applicable to blue or green money willow, can promote the sprouting of blue or green money willow seed, thus obtain higher germination rate.
The technical scheme that the present invention solves its technical problem is: a kind of trees urge kind of the method sprouted, and it is characterized in that, step is: (1) seed soaking: application of temperature is the seed-soaking liquid of 30 DEG C, carries out seed soaking 9h; The composition of wherein said seed-soaking liquid and preparation method are: by NaCl0.6g, KCl0.98g, CaCl 22H 2o0.17g, MgCl 26H 2o0.11g, methacrylate alcohol polyoxyethylene ester 30mg, γ-aminobutyric acid 40mg, astaxanthin 0.2g join in 1000ml distilled water and make suspension; (2) vernalization process: insulation vernalization built by blue or green money willow seed straw step (1) obtained, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
Dormancy is an important stage in life cycle, to plant, there is important ecological significance, seed time of " correctly " in favourable habitat sprouts and is not only conducive to seedling survival, and species can be enable successfully to settle down and long-term its population of maintenance.Inventor studies discovery, the blue or green money willow seed dormancy phase is long, irregular reason of germinateing is that its Some seeds still keeps resting state under optimum conditions, also namely all can not once sprout completely under what conditions with batch seed, sprouting phenomenon of postponing this kind reduces the mortality risk in seed germination and seedling establishment process by temporal diffusion, keep race's continuity, that this is the population extinction avoided occurred in a kind of natural evolution, careful germination decision.The research of prior art mainly concentrates on seed coat poor air permeability and exists in Germination Inhibitors both direction: blue or green money willow seed coat structure is fine and close, gas permeability, water penetration inequality; Germination Inhibitors, in embryo, is passed in time and to be exosmosed dissipation by seed coat gradually; Seed coat is role on delay is sprouted, and is the sprouting that inhibit seed by penetrating of oxygen of restriction on the one hand, hinders the dissipation of exosmosing of Germination Inhibitors on the other hand.
And the research of inventor finds that the material base of this factor foundation is the otherness of seed coat physical arrangement, under microscope, there are many ducts, have agglomerate sample filler in cell channels in blue or green money willow seed seed coat section display in seed coat.Histologic section is added up, with the agglomerate sample filler reflecting point quantity variance comparatively large (P<0.05) of batch seed with time point, the passing agglomerate sample filler reflecting point quantity put in time with batch seed reduces (P<0.05) gradually, seal with wax with section before monomer seed coat water soaking water soaking after 1 hour, section is sealed with wax after plant ash solution floods 1 hour and is compared, and histologic section statistics shows filler reflecting point quantity variance not statistically significant (P>0.05).Can reason out according to this Basic Experiment Study result: in this kind of duct, agglomerate sample filler affects the factor that Germination Inhibitors exosmosed by seed coat, and cause with not being both of agglomerate sample filler content in batch seed irregular reason of germinateing.
Current universal method is: sowing time, soaked seed before sowing 2d, then build insulation vernalization with straw, and temperature remains on 20-30 DEG C in 2 ~ mid-March.Timing is stirred and is mixed thoroughly, and add the hot water of pouring about 40 DEG C, 4-5d seed shows money or valuables one carries unintentionally, and the seed that shows money or valuables one carries unintentionally should be selected to sow in mid-February.Also have report, can about 15d be done sth. in advance by the seed sprouting of acid etching method vernalization, but most high germination rate is still less than 60%, preserve percentage of seedgermination within 1 year less than 50%.Its reason is, immersion can not change interior agglomerate sample filler content, the seed that only can decline for script agglomerate sample filler content is by soaking, accelerate the former Germination Inhibitors oozed out of seed coat to eliminate, still there is seed germination time disunity, percentage of seedgermination is difficult to problems such as effectively increasing substantially, limit blue or green money willow genetic breeding and production application.
Compared with prior art, the present invention has following outstanding beneficial effect.
1, reasonably fill a prescription: can accelerate to exosmose by the effect of inside and outside ion concentration osmotic pressure; Infrastest shows: seal with wax after methacrylate alcohol polyoxyethylene ester solution soaks 1 hour with section before monomer seed coat methacrylate alcohol polyoxyethylene ester solution soaks and compare, and histologic section statistics display filler reflecting point quantity reduces 23.47%; Astaxanthin contributes to increasing this kind of chemical stimulation and to sow hypodermic steadiness, and the effect of γ-aminobutyric acid is also to increase seed coat to the adaptability of environment.
2, raw materials used inanimate object hormonal action, while the blue or green money willow seed germination of promotion, can not disturb growth of seedling state, can not reduce blue or green money willow seedling chilling resistance.
3, seed germination rate then and planting percent can be significantly improved, shorten the seed seedling-raising cycle, reduce production cost, there is volume increase, the advantage such as of fine quality, efficient, inexpensive, for the development of blue or green money willow related industry provides material supply.
4, this kind of seed-soaking liquid is used for new seed (seed within 1 year storage life) simultaneously effectively, has practical significance for the production cycle shortening the seed growing of blue or green money willow seedling.
Above beneficial effect is experiment and confirms, experimental technique and the results are shown in embodiment.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
One, one is tested: for the blue or green money willow seed of storage life at 1 year ~ 2 years.
(1) materials and methods.
1, collect seed: be gather ripe blue or green money willow seed from Nanjing in 2011.
Acquisition process process adopts following traditional approach: after Fruit returns, and cold water dipping, rubs with the hands and remove pericarp, wash down, then the surface of the seed grease plant ash solution flooded with water immediately, and clear water is cleaned and dried in the shade, and then hides in shady and cool indoor sand of ventilating.
2, breeding sprout: on March 14th, 2012, begin one's study in area, Junan, Linyi, temperature 16 DEG C day/3 DEG C of nights.Seed is divided into 5 groups at random: acid etching control group, base extraction group, optimization process group, simple seed-soaking liquid group, optimization process+seed-soaking liquid group, often organizes 100.
2.1 acid etching control groups adopt literature procedure:
(1) seed soaking: the application concentrated sulfuric acid soaks seed 9h;
(2) vernalization process: blue or green money willow seed 15 DEG C of clean water step (1) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 10 days.
2.2 base extraction groups are embodiment 1 scheme:
(1) dripping concentration in the clear water of seed soaking: 1000ml is that the pH value of this solution is adjusted to 8.6 by the NaOH solution of 1N, and temperature is 30 DEG C, applies this dipping by lye pretreatment seed 9h;
(2) vernalization process: blue or green money willow seed 15 DEG C of clean water step (1) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
2.3 optimization process groups are embodiment 2 scheme:
(1) alkalize seed soaking: 1000ml clear water in drip concentration be that the pH value of this solution is adjusted to 8.6 by the NaOH solution of 1N, apply this dipping by lye pretreatment seed 9h;
(2) the rich nitrogen process of vibration decompression: oscillator is cultivated in the constant temperature clear water water-bath that blue or green money willow seed step (1) obtained puts into sealing, and extraction section air, adds nitrogen, reduce air pressure, reaching air pressure is 0.07MPa, when nitrogen content is 85%, starts oscillation treatment, oscillation treatment temperature 30 DEG C, vibration rotating speed 20r/min, time 0.5h, opens air valve and fully takes a breath, until gas is atmospheric air in oscillator, keep concussion 12h;
(3) vernalization process: blue or green money willow seed 15 DEG C of clean water step (2) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
2.4 simple seed-soaking liquid groups are embodiment 3 scheme:
(1) seed soaking: application of temperature is the seed-soaking liquid of 30 DEG C, carries out seed soaking 9h;
(2) vernalization process: insulation vernalization built by blue or green money willow seed straw step (1) obtained, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
The composition of described seed-soaking liquid and preparation method are: by NaCl0.6g, KCl0.98g, CaCl 22H 2o0.17g, MgCl 26H 2o0.11g, methacrylate alcohol polyoxyethylene ester 30mg, γ-aminobutyric acid 40mg, astaxanthin 0.2g join in 1000ml distilled water and make suspension.
2.5 optimization processes+seed-soaking liquid group is embodiment 4 scheme:
(1) alkalize seed soaking: 1000ml clear water in add seed-soaking liquid, apply this seed-soaking liquid dipping pretreatment seed 9h;
(2) vibration reduced pressure treatment: oscillator is cultivated in the constant temperature clear water water-bath that blue or green money willow seed step (1) obtained puts into sealing, extraction section air, adds nitrogen, reduces air pressure, reaching air pressure is 0.07MPa, when nitrogen content is 85%, start oscillation treatment, oscillation treatment temperature 30 DEG C, vibration rotating speed 20r/min, time 0.5h, opens air valve and fully takes a breath, and keeps concussion 12h;
(3) vernalization process: blue or green money willow seed 15 DEG C of clean water step (2) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
The composition of described seed-soaking liquid and preparation method are: by NaCl0.6g, KCl0.98g, CaCl 22H 2o0.17g, MgCl 26H 2o0.11g, methacrylate alcohol polyoxyethylene ester 30mg, γ-aminobutyric acid 40mg, astaxanthin 0.2g join 1000ml distilled water and make suspension.
3, drilling is germinateed: gained seed after above-mentioned each group of process is carried out drilling, sowing furrow width 4cm, dark 3cm, and line-spacing spacing in the rows is 25cm, thickness of earth covering 2cm, and every day, trickle kept bed surface water content 35% ~ 40%.
Within 15 days, measure each group of germination rate later, after 3 months, measure the strain number that height of seedling is greater than 10cm.
(2) result.
1, germination rate: as can be seen from Table 1, the impact on blue or green money willow percentage of seedgermination then of 5 kinds of different disposal methods has very big-difference.
Display is compared: alkali lye control group germination rate is higher than acid etching control group between 1.1 groups, difference has statistical significance (P=0.047, card side=3.934), show to adopt alkaline solution process to destroy blue or green money willow seed skin structure, can promote that it is to moisture absorption, but germination rate is apparently higher than acid etching control group, show that its effect is not restricted to alkalescence and destroys physical arrangement, and may be relevant with its decomposition Germination Inhibitors.
Compare display between 1.2 groups: optimization process group (embodiment 2) germination rate is higher than base extraction group, and difference has statistical significance (P=0.015, card side=5.882), show to adopt the rich nitrogen optimized treatment method of alkalization vibration decompression to be better than the scheme that alkalizes merely.Its effect link may be: by the Germination Inhibitors passing through in embryo to ooze out outside the duct of kind of intracutaneous without the filling of open agglomerate sample filler before destroying by alkalizing, pass through the rich nitrogen of decompression afterwards in order to destroy agglomerate sample filler physicalchemical structure, accelerate the outer row of Germination Inhibitors, rear recovery normal pressure and air supply, make seed obtain good oxygen to exchange, and make Germination Inhibitors depart from seed coat by persistent oscillation; Seed through above-mentioned process has effectively departed from dormancy suppression, sprouts at suitable temperature and illumination.
Display is compared: simple seed-soaking liquid group (embodiment 3) germination rate is higher than base extraction group between 1.3 groups, difference has statistical significance (P=0.035, card side=4.432), show that this kind of seed-soaking liquid helps lend some impetus to seed germination, and this kind promotes it is not merely the adjustment due to pH value.Its approach may be to accelerate to exosmose by the effect of inside and outside ion concentration osmotic pressure; Infrastest shows that monomer seed coat methacrylate alcohol polyoxyethylene ester solution soaks front sealing with wax after methacrylate alcohol polyoxyethylene ester solution soaks 1 hour with section and compares, and histologic section statistics display filler reflecting point quantity reduces 23.47%; Astaxanthin contributes to increasing this kind of chemical stimulation and to sow hypodermic steadiness, and the effect of γ-aminobutyric acid is also to increase seed coat to the adaptability of environment.
Display is compared: optimization process group (embodiment 2) germination rate is higher than simple seed-soaking liquid group (embodiment 3) between 1.4 groups, no significant difference (P=0.743, card side=0.107), show to adopt the rich nitrogen optimized treatment method of alkalization vibration decompression and this kind of seed-soaking liquid to urge to sprout effect suitable.
Display is compared: optimization process+seed-soaking liquid group (embodiment 4) germination rate is higher than optimization process group (embodiment 2), and difference has statistical significance (P=0.027, card side=4.878) between 1.5 groups.
Display is compared: optimization process+seed-soaking liquid group (embodiment 4) germination rate is higher than simple seed-soaking liquid group (embodiment 3), and difference has statistical significance (P=0.012, card side=6.368) between 1.6 groups.This programme is optimum optimization scheme, illustrates that the seed-soaking liquid described in blue or green money willow seed application coordinates the process of alkalization vibration decompression double optimization, can suppress by breaking dormancy faster, promote to sprout, have the advantages that the cultivation time is short, germination rate is high, survival rate is high.
2, drilling after 3 months height of seedling be greater than the percentage comparisons of 10cm strain number: as can be seen from Table 1, base extraction group (embodiment 1) blue or green money willow then seed all apparently higher than acid etching control group (P<0.05), and optimization process group (embodiment 2), simple seed-soaking liquid group (embodiment 3), optimization process+seed-soaking liquid group (embodiment 4) compare with acid etching control group, then difference extremely obviously (P<0.01).
The each processing method of table 1 affects for the sprouting of blue or green money willow new seed
Group number Process Germination rate (%) Height of seedling > 10cm ratio (%)
1 Acid etching control group 46 41
2 Base extraction group 60 53
3 Optimization process group 76 73
4 Simple seed-soaking liquid group 74 72
5 Optimization process+seed-soaking liquid group 88 85
Two, two are tested: for the blue or green money willow seed of storage life at 1 year ~ 2 years.
(1) materials and methods
1, collect seed: be gather ripe blue or green money willow seed from Nanjing in 2011.
Acquisition process process adopts following traditional approach: after Fruit returns, and cold water dipping, rubs with the hands and remove pericarp, wash down with water immediately, then by the surface of the seed grease, with plant ash solution dipping, clear water is cleaned and dried in the shade, and then hides in shady and cool indoor sand of ventilating.
2, breeding sprout: on March 15th, 2013, begin one's study in area, Junan, Linyi, temperature 14 DEG C day/4 DEG C of nights.Seed is divided into 5 groups at random: acid etching control group, base extraction group, optimization process group, simple seed-soaking liquid group, optimization process+seed-soaking liquid group, often organizes 100.
2.1 acid etching control groups adopt literature procedure:
(1) seed soaking: the application concentrated sulfuric acid soaks seed 9h;
(2) vernalization process: blue or green money willow seed 15 DEG C of clean water step (1) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 10 days.
2.2 base extraction groups are embodiment 5 scheme:
(1) dripping concentration in the clear water of seed soaking: 1000ml is that the pH value of this solution is adjusted to 8.2 by the NaOH solution of 1N, and temperature is 30 DEG C, applies this dipping by lye pretreatment seed 9h;
(2) vernalization process: blue or green money willow seed 15 DEG C of clean water step (1) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
2.3 optimization process groups are embodiment 6 scheme:
(1) alkalize seed soaking: 1000ml clear water in drip concentration be that the pH value of this solution is adjusted to 8.2 by the NaOH solution of 1N, apply this dipping by lye pretreatment seed 9h;
(2) the rich nitrogen process of vibration decompression: oscillator is cultivated in the constant temperature clear water water-bath that blue or green money willow seed step (1) obtained puts into sealing, and extraction section air, adds nitrogen, reduce air pressure, reaching air pressure is 0.06MPa, when nitrogen content is 90%, starts oscillation treatment, oscillation treatment temperature 40 DEG C, vibration rotating speed 15r/min, time 0.5h, opens air valve and fully takes a breath, until gas is atmospheric air in oscillator, keep concussion 12h;
(3) vernalization process: blue or green money willow seed 15 DEG C of clean water step (2) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
2.4 simple seed-soaking liquid groups are embodiment 7 scheme:
(1) seed soaking: application of temperature is the seed-soaking liquid of 30 DEG C, carries out seed soaking 9h;
(2) vernalization process: insulation vernalization built by blue or green money willow seed straw step (1) obtained, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
The composition of described seed-soaking liquid and preparation method are: by NaCl0.6g, KCl0.98g, CaCl 22H 2o0.17g, MgCl 26H 2o0.11g, methacrylate alcohol polyoxyethylene ester 30mg, γ-aminobutyric acid 40mg, astaxanthin 0.2g join in 1000ml distilled water and make suspension.
2.5 optimization processes+seed-soaking liquid group is embodiment 8 scheme:
(1) alkalize seed soaking: 1000ml clear water in add seed-soaking liquid, apply this seed-soaking liquid dipping pretreatment seed 9h;
(2) vibration reduced pressure treatment: oscillator is cultivated in the constant temperature clear water water-bath that blue or green money willow seed step (1) obtained puts into sealing, extraction section air, reaching air pressure is 0.06MPa, start oscillation treatment, oscillation treatment temperature 40 DEG C, vibration rotating speed 10r/min, time 0.5h, open air valve fully to take a breath, keep concussion 12h;
(3) vernalization process: blue or green money willow seed 15 DEG C of clean water step (2) obtained, natural seasoning, insulation vernalization built by straw, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
The composition of described seed-soaking liquid and preparation method are: by NaCl0.6g, KCl0.98g, CaCl 22H 2o0.17g, MgCl 26H 2o0.11g, methacrylate alcohol polyoxyethylene ester 40mg, γ-aminobutyric acid 40mg, astaxanthin 0.2g join 1000ml distilled water and make suspension.
3, drilling is germinateed: gained seed after above-mentioned each group of process is carried out drilling, sowing furrow width 4cm, dark 3cm, and line-spacing spacing in the rows is 25cm, thickness of earth covering 2cm, and every day, trickle kept bed surface water content 35% ~ 40%.
Within 15 days, measure each group of germination rate later, after 3 months, measure the strain number that height of seedling is greater than 10cm.
(2) result.
1, germination rate: as can be seen from Table 2,5 kinds of different disposal method impacts on blue or green money willow percentage of seedgermination have very big-difference.
Display is compared: alkali lye control group (embodiment 5) germination rate is higher than acid etching control group, and difference has statistical significance (P=0.036, card side=4.367) between 1.1 groups.
Display is compared: optimization process group (embodiment 6) germination rate is apparently higher than acid etching control group, and difference has obvious statistical significance (P<0.001, card side=12.718) between 1.2 groups.
Display is compared: simple seed-soaking liquid group (embodiment 7) germination rate is apparently higher than acid etching control group, and difference has obvious statistical significance (P<0.001, card side=13.987) between 1.3 groups.
Display is compared: optimization process group (embodiment 6), simple seed-soaking liquid group (embodiment 7) germination rate are higher than alkali lye control group (embodiment 5) between 1.4 groups, but this difference not statistically significant (P>0.05); Compare between optimization process group (embodiment 6) with simple seed-soaking liquid group (embodiment 7), difference not obvious (P>0.05);
Display is compared: optimization process+seed-soaking liquid group (embodiment 8) germination rate is apparently higher than alkali lye control group (embodiment 5) between 1.5 groups, difference has statistical significance (P=0.004, card side=8.317), but and optimization process group (embodiment 6), merely difference not obvious (P>0.05) between seed-soaking liquid group (embodiment 7).
3, drilling after 3 months height of seedling be greater than the percentage comparisons of 10cm strain number: as can be seen from Table 2, alkali lye control group (embodiment 5) blue or green money willow seed ratio is all apparently higher than acid etching control group (P<0.05), and optimization process group (embodiment 6), simple seed-soaking liquid group (embodiment 7), optimization process+seed-soaking liquid group (embodiment 8) compare with acid etching control group, then difference extremely obviously (P<0.01).
The each processing method of table 2 affects for the sprouting of blue or green money willow year old seeds
Group number Process Germination rate (%) Height of seedling > 10cm ratio (%)
6 Acid etching control group 59 54
7 Base extraction group 73 68
8 Optimization process group 82 78
9 Simple seed-soaking liquid group 83 79
10 Optimization process+seed-soaking liquid group 89 88
(3) conclusion.
A kind of special seed soaking agent being applicable to blue or green money willow involved in the present invention, can promote the sprouting of blue or green money willow seed, thus obtains higher germination rate, and under relative low temperature environment, can obtain higher survival rate of seedling.
The present invention is simple to operate, standard is unified, be easy to grasp, economic and practical, be both applicable to the experiment of a small amount of in scientific research blue or green money willow seed sample, be also applicable to a large amount of blue or green money willow seed treatment in production process.
It should be noted that; particular of the present invention is to invention has been detailed description; for a person skilled in the art, the various apparent change carried out it when not deviating from the spirit and scope of the present invention is all within protection scope of the present invention.

Claims (1)

1. trees urge kind of the method sprouted, and it is characterized in that, step is: (1) seed soaking: application of temperature is the seed-soaking liquid of 30 DEG C, carries out seed soaking 9h; The composition of wherein said seed-soaking liquid and preparation method are: by NaCl0.6g, KCl0.98g, CaCl 22H 2o0.17g, MgCl 26H 2o0.11g, methacrylate alcohol polyoxyethylene ester 30mg, γ-aminobutyric acid 40mg, astaxanthin 0.2g join in 1000ml distilled water and make suspension; (2) vernalization process: insulation vernalization built by blue or green money willow seed straw step (1) obtained, and temperature remains on 20 DEG C, and humidity remains on 60%, preserves 8 days.
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