CN111848227A - Dandelion seed soaking liquid and application method thereof - Google Patents
Dandelion seed soaking liquid and application method thereof Download PDFInfo
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- CN111848227A CN111848227A CN202010765649.8A CN202010765649A CN111848227A CN 111848227 A CN111848227 A CN 111848227A CN 202010765649 A CN202010765649 A CN 202010765649A CN 111848227 A CN111848227 A CN 111848227A
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- dandelion
- nitrate
- seed soaking
- zinc
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a dandelion seed soaking liquid and an application method thereof, wherein the dandelion seed soaking liquid contains trace elements of zinc, manganese and copper, and major elements of nitrogen and potassium. The total content of the trace elements of zinc, copper and manganese, namely Zn, Cu and Mn, is more than or equal to 100 g/L; the total content of the macroelements of nitrogen and potassium is N + K2O is more than or equal to 80 g/L. The seed soaking liquid can obviously improve the germination rate, the germination potential, the peroxidase activity and the catalase activity of the seeds.
Description
Technical Field
The invention relates to a dandelion seed soaking liquid meeting the microelement water-soluble fertilizer standard and an application method thereof.
Background
The dandelion belongs to perennial herb of Compositae, is named as Huanghualang, herba Veronicae Divaricatae, milk juice grass and the like, is bitter, sweet and cold in taste, enters liver and stomach channels, and has the effects of resisting oxidation, resisting tumors, enhancing immunity, inhibiting bacteria and the like. The dandelion has good taste and rich nutrition, is a health-care vegetable, and is one of hundreds of plants which are homologous in medicine and food and are listed by the national committee. With the gradual understanding of people on the health care efficacy of the dandelion, the utilization of the dandelion begins to develop into diversification of Chinese patent medicines, beverages, tea leaves and the like, the demand is increased year by year, while the wild resources of the dandelion are reduced year by year, so that the artificial planting of the dandelion has higher economic benefit and good development prospect. However, from the start of sowing to the end of emergence, the seeds remain in the soil environment for a considerable period of time, which is variable or even unfavorable, and measures are therefore required to increase the rate of emergence of the sprouts. Proper seed treatment before sowing is beneficial to complete seedlings, uniform seedlings, even seedlings and strong seedlings, and is one of effective measures for improving the yield.
In recent years, researchers have paid attention to the content of trace elements in the dandelion, and researches have shown that the dandelion contains a large amount of potassium, the essential trace nutrient elements are mainly zinc, manganese, copper and iron, the average content ratio of zinc (Zn) to copper (Cu) is about 3, the application of a proper amount of nitrogen fertilizer can provide high yield and quality of the dandelion, and the optimal nitrogen (N) application amount is 73.6-110.4kg/hm2. Cultivated lands lack trace elements of iron, copper, manganese and zinc in China account for 5%, 6.9%, 34.5% and 51.5% respectively, but iron-deficient soil in traditional Chinese medicine cultivation bases is relatively less. Therefore, Zn (NO) is mainly developed aiming at the nutrition characteristics of dandelion3)2、Cu(NO3)2KNO in constant concentration system3-Mn(NO3)2-H2And (3) performing O-phase balance research, and preparing dandelion soaking liquid meeting the microelement water-soluble fertilizer standard (NY1428-2010) according to a phase diagram.
Disclosure of Invention
The invention aims at solving the nutrition characteristics of dandelion and the practical problems in the seedling raising process, and aims to provide a dandelion seed soaking liquid for improving the germination rate and the vitality of seeds and an application method thereof.
The dandelion seed soaking liquid contains trace elements of zinc, manganese and copper and major elements of nitrogen and potassium.
In the nutrient elements contained in the dandelion seed soaking liquid, zinc is derived from zinc nitrate; the manganese is derived from manganese nitrate; the copper is derived from copper nitrate; potassium is derived from potassium nitrate; the nitrogen is from potassium nitrate, zinc nitrate, manganese nitrate, and copper nitrate.
The total content of the trace elements of zinc, copper and manganese, namely Zn, Cu and Mn, is more than or equal to 100g/L, wherein: Zn-Cu ═ 1 (2.9-3.1), (Zn + Cu): Mn ═ 1 (0.8-1.3). The total content of the macroelements of nitrogen and potassium is N + K2O is more than or equal to 80g/L, and N is K2O=(1.7-2.6):1。
The proportion of the trace elements of zinc, copper and manganese is preferably (Zn + Cu) and Mn is 1 (1.0-1.1); the preferred proportion of the macroelements of nitrogen and potassium is N: K2O=(1.9-2.4):1。
The application method of the dandelion seed soaking liquid comprises the following steps:
1. diluting the seed soaking liquid with water by about 200 times, and soaking the seed.
2. When soaking seeds, the proportion of the seeds and the diluent is controlled to be about 1g to 100mL, and the seed soaking time is controlled to be 7.5-8.5 h.
3. Because dandelion seeds are light and not easy to sink into the solution, the dandelion seeds are preferably shaken for 2-3 times during the seed soaking period.
The seed soaking liquid can obviously improve the germination rate, the germination potential, the peroxidase activity and the catalase activity of the seeds.
Drawings
FIG. 1 is a graph showing the effect of various seed soaking dilutions on the germination rate of dandelion.
FIG. 2 is a graph showing the effect of various seed soaking dilutions on the germination potential of dandelion.
FIG. 3 is a graph showing the effect of various seed soaking dilutions on dandelion seedling length.
FIG. 4 is a graph showing the effect of various seed soaking dilutions on CAT activity of dandelion seedlings.
FIG. 5 is a graph showing the effect of various seed soaking dilutions on POD activity of dandelion seedlings.
Detailed Description
1. Raw material preparation
Zinc nitrate (HG/T3582-2009, Zn (NO)3)2·6H2O content is more than or equal to 98 percent), copper nitrate (HG/T3443-3)2·3H2O content is more than or equal to 99 percent), manganese nitrate (HG/T3817-3)2The content is more than or equal to 50 percent) and potassium nitrate (GB/T20784-2O content is more than or equal to 46 percent, N content is more than or equal to 13.5 percent, nitric acid (GB/T337.2-2014, HNO)3Content not less than 60%), water.
2. Preparation process
(1) Adding metered water into a reaction kettle, and properly heating when the atmospheric temperature is lower than 20 ℃, but the temperature is not higher than 60 ℃;
(2) sequentially adding potassium nitrate, zinc nitrate and manganese nitrate, stirring to accelerate dissolution, wherein the feeding interval is determined according to the feeding amount, and is generally 30min/100 kg; testing the pH value after the potassium nitrate, the zinc nitrate and the manganese nitrate are completely dissolved, adjusting the pH value to 3.5-4.0 by using nitric acid when the pH value is higher than 4.0, adding copper nitrate, and stirring until the potassium nitrate, the zinc nitrate and the manganese nitrate are completely dissolved;
(3) filtering to remove water insoluble substance to obtain herba Taraxaci extract solution.
3. Seed soaking case
Case 1 (F): 597.4g of water, 52.4g of potassium nitrate, 209.0g of zinc nitrate, 358.2g of manganese nitrate and 58.0g of copper nitrate; the obtained herba Taraxaci soaking liquid is measured by atomic absorption spectrophotometry to obtain Zn concentrations2+=45.01g/L,Cu2+=15.09g/L,Mn2+=54.99g/L,K2O=24.09g/L,N=61.12g/L。
Wherein: 115.09g/L Zn + Cu, 2.98:1 Zn Cu, 1:0.92 Mn, and N + K2O=85.21g/L,N:K2O=2.54:1。
Case 2 (G): 556.9g of water, 65.5g of potassium nitrate, 209.0g of zinc nitrate, 390.5g of manganese nitrate and 58.0g of copper nitrate; the obtained herba Taraxaci soaking liquid is measured by atomic absorption spectrophotometry to obtain Zn concentrations2+=44.99g/L,Cu2+=14.58g/L,Mn2+=59.96g/L,K2O=30.12g/L,N=65.46g/L。
Wherein: 119.53g/L Zn + Cu + Mn, 3.09:1 Zn: Cu, (1: 1.01 Mn, N + K)2O=95.58g/L,N:K2O=2.2:1。
Case 3 (H): 512.8g of water, 78.6g of potassium nitrate, 209.0g of zinc nitrate, 423.4g of manganese nitrate and 58.0g of copper nitrate; the obtained herba Taraxaci soaking liquid is measured by atomic absorption spectrophotometry to obtain Zn concentrations2+=45.29g/L,Cu2+=15.33g/L,Mn2+=65.00g/L,K2O=36.14g/L,N=69.80g/L。
Wherein: 125.62g/L Zn + Cu + Mn, 2.95:1 Zn: Cu, (1: 1.07 Mn, N + K)2O105.94 g/L and N K2O=1.93:1。
Case 4 (I): 475.9g of water, 91.7g of potassium nitrate, 209.0g of zinc nitrate, 455.4g of manganese nitrate and 58.0g of copper nitrate; measuring the obtained herba Taraxaci soaking liquid by atomic absorption spectrophotometryThe concentrations are determined to be Zn respectively2+=45.30g/L,Cu2+=15.21g/L,Mn2+=69.91g/L,K2O=42.16g/L,N=74.14g/L。
Wherein: 130.42g/L Zn + Cu, 2.98:1 Zn: Cu, (1: 1.16 Mn, N + K)2O116.3 g/L and N K2O=1.76:1。
4. Seed soaking treatment
Step 1: taking four seed soaking liquids of case 1(F), case 2(G), case 3(H) and case 4(I), and diluting with water by 10 times, 50 times, 100 times, 200 times and 500 times respectively to obtain seed soaking diluent;
step 2: 0.2g of dandelion seeds with uniform size and full particles are selected and respectively soaked in 20mL of soaking dilution and 20mL of clear water;
and step 3: the soaking time is 8h, and the mixture is slightly shaken for 1 time every 2 hours.
5. Germination test
Step 1: filtering the soaked seeds by using gauze, and sucking free water on the surfaces of the seeds by using filter paper;
step 2: uniformly placing the dandelion seeds on two layers of filter paper wetted by clear water in culture dishes, and uniformly placing 60 dandelion seeds in each culture dish;
and step 3: placing the culture dish into an intelligent artificial climate incubator, setting the temperature at 25 ℃, and keeping the temperature free of illumination and natural humidity;
and 4, step 4: recording the germination number every day, dripping clear water to keep the filter paper moist, and continuously operating for 14 days;
and 5: the germination rate and the germination potential were calculated, the seedling length was measured, and the peroxidase activity and the catalase activity were measured.
6. Test results
The effect of various seed soaking dilutions on the germination rate of dandelion is shown in FIG. 1, the effect on the germination vigor is shown in FIG. 2, the effect on the seedling length is shown in FIG. 3, the effect on the peroxidase activity is shown in FIG. 4, and the effect on the catalase activity is shown in FIG. 5.
As can be seen from FIGS. 1 to 5, the four seed soaking solutions all promoted the germination percentage of dandelion within a certain concentration range, compared with the clear water control,Germination potential and seedling growth, and improving peroxidase and catalase activity. Wherein, Zn-Cu-Mn-K2The dandelion seed soaking liquid with O-N which is approximately equal to 45-15-60-30-65(g/L) has the best soaking effect by 200 times of the diluent.
Claims (7)
1. A dandelion seed soaking liquid is characterized in that:
the dandelion seed soaking liquid contains trace elements of zinc, manganese and copper and major elements of nitrogen and potassium.
2. The dandelion infusion of claim 1, wherein:
the total content of the trace elements of zinc, copper and manganese, namely Zn, Cu and Mn, is more than or equal to 100 g/L; the total content of the macroelements of nitrogen and potassium is N + K2O≥80g/L。
3. The dandelion infusion of claim 1, wherein:
in the trace elements, Zn, Cu (2.9-3.1) and 1, (Zn + Cu) and Mn (1) (0.8-1.3); in the macroelements, N is K2O=(1.7-2.6):1。
4. The dandelion infusion of claim 3, wherein:
in the trace elements, the proportion of zinc, copper and manganese is (Zn + Cu), and Mn is 1 (1.0-1.1); in the macroelements, the ratio of nitrogen to potassium is N: K2O=(1.9-2.4):1。
5. The dandelion infusion of claim 1, 2 or 3, wherein:
in the nutrient elements contained in the dandelion seed soaking liquid, zinc is derived from zinc nitrate; the manganese is derived from manganese nitrate; the copper is derived from copper nitrate; potassium is derived from potassium nitrate; the nitrogen is from potassium nitrate, zinc nitrate, manganese nitrate, and copper nitrate.
6. A method for applying the dandelion infusion of claim 1, 2 or 3, characterized in that:
diluting the seed soaking liquid with water by about 200 times, and soaking the seed.
7. The method of application according to claim 6, characterized in that:
when soaking seeds, the proportion of the seeds and the diluent is controlled at 1g:100mL, and the seed soaking time is controlled at 7.5-8.5 h.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115745685A (en) * | 2022-10-31 | 2023-03-07 | 合肥工业大学 | Wheat seed soaking liquid containing trace elements in alginic acid and preparation method and application thereof |
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CN1393428A (en) * | 2001-06-28 | 2003-01-29 | 万怡震 | Plant balanced nutrient solution |
CN102326586A (en) * | 2011-07-07 | 2012-01-25 | 云南师范大学 | Jatropha curcas L. germination-accelerating stress tolerance seed soaking agent and preparation method thereof |
CN103478166A (en) * | 2013-10-11 | 2014-01-01 | 王春爱 | Seed soaking agent for promoting germination of Folium Cylocaryae Paliuri seeds |
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Patent Citations (3)
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CN1393428A (en) * | 2001-06-28 | 2003-01-29 | 万怡震 | Plant balanced nutrient solution |
CN102326586A (en) * | 2011-07-07 | 2012-01-25 | 云南师范大学 | Jatropha curcas L. germination-accelerating stress tolerance seed soaking agent and preparation method thereof |
CN103478166A (en) * | 2013-10-11 | 2014-01-01 | 王春爱 | Seed soaking agent for promoting germination of Folium Cylocaryae Paliuri seeds |
Non-Patent Citations (1)
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CN115745685A (en) * | 2022-10-31 | 2023-03-07 | 合肥工业大学 | Wheat seed soaking liquid containing trace elements in alginic acid and preparation method and application thereof |
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