CN106961873A - A kind of method for promoting Paeonia suffruticosa seed to take root - Google Patents
A kind of method for promoting Paeonia suffruticosa seed to take root Download PDFInfo
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- CN106961873A CN106961873A CN201710222817.7A CN201710222817A CN106961873A CN 106961873 A CN106961873 A CN 106961873A CN 201710222817 A CN201710222817 A CN 201710222817A CN 106961873 A CN106961873 A CN 106961873A
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- Prior art keywords
- seed
- paeonia suffruticosa
- promoting
- cacl
- take root
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/04—Arranging seed on carriers, e.g. on tapes, on cords ; Carrier compositions
- A01C1/044—Sheets, multiple sheets or mats
Abstract
The invention discloses a kind of method for promoting Paeonia suffruticosa seed to take root, this method is by temperature control and the synergy of calcium chloride, the rooting rate and quality of rooting of seed are substantially increased while breaking seed dormancy, harm of the potential chemical agent residual to plant strain growth is not only prevented, and input cost can be reduced, whole method is easy to operate, efficiency high, the plantation popularization and the research and development of functional product of ecosystem organic farm products can be effectively facilitated, the technological gap that simplified method improves Paeonia suffruticosa seed rooting rate and quality of rooting has been filled up.
Description
Technical field
The invention belongs to field of agricultural production technologies, a kind of method taken root in particular to seed, more particularly to one
Plant the method for promoting Paeonia suffruticosa seed to take root.
Background technology
Tree peony (Paeonia suffruticosa) belongs to Paeoniaceae (Paeoniaceae) Paeonia (Paeonia) tree peony
The semishrub plant of group (Paeonia sect.Moutan DC.), is world-renowned flowers, is also one of Chinese ten big famous flowers,
Because its is beautiful in colour, jade laughs at pearl perfume, splendid, have the good reputation of " king in spending ", in the existing thousands of years natural life of China
The long artificial cultivation history with more than 1500 years, and the areas such as Japan, Europe and the U.S. are introduced a fine variety already.Tree peony also view and admire because great and
Medical value and be known, recent studies have found that oil content is higher in peony seeds benevolence, more than 22%, and rich in amino
Acid, protein, polysaccharide, carotenoid, trace element and vitamin group and a variety of unrighted acids, especially alpha-linolenic acid
Content new resource food be approved as by former health ministry more than 42.35%, 2011 year (defend food newly logical word (2011) the 9th
Number)；The General Office of the State Council issues within 2014《Opinion on accelerating woody oleiferous plants industry development》(Office of the State Council's hair [2014] 68
Number), using oil with tree peony as emphasis promotion traditional oil tree.Tree peony as integrate view and admire, medicinal and oily spy
Color economic plants, is widelyd popularize plantation.2014, tree peony 22 provinces in the whole nation, 200 Duo Ge counties cultivated area up to 6.67 ten thousand
hm2, develop into 13.33 ten thousand hm within 20152, the principal item of plantation is ' Feng Dan is white ' and ' purple plague purpura '.
At present, nursery is generally carried out to tree peony using the method for seeding and seedling raising in agricultural production and breeding expands numerous, but it is male
Red seed is taken root difficulty, and rooting rate is low, and the development of tree peony industry is seriously hindered.On the one hand be due to external source beyond embryo because
The limitation of element, including endosperm, the physical barriers for planting skin and pericarp formation, cause the bad hydraulic permeability of seed；Or exist in kind skin
Suppress the chemical substance of germination；On the other hand the reason for being due to embryo itself, causes：Paeonia seed has typical
Embryo dormancy characteristic, the dual dormancy including radicle (hypocotyl) and plumule (epicotyl), especially hypocotyl dormancy, embryo
Form and physiology it is immature, it is necessary to endosperm provide nutriment, complete it and grow；Lack hormone or presence that regulation and control are taken root
Suppress the chemical substance taken root of seed, such as the nitrogen-containing compound rich in ammonia, cumarin, abscisic acid, citric acid, acetic acid tartaric acid,
Salicylic acid, butenoic acid, malic acid, aliphatic acid and citral etc., often a variety of mortifiers synergy suppress seeds and take root.Beat
The method of broken seed dormancy mainly has physics and chemical method.Physical mainly include temperature, machinery, ultrasonic wave, ray, electric field and
Stratificated treatment etc.；Chemical method includes Inorganic chemicals, organic chemistry medicament and HORMONE TREATMENT.The processing of Physical is conducive to embryo
The germination of root, but rooting rate has much room for improvement, chemical treatment such as gibberellin (gibberellin A3,GA3) seed can be significantly improved
Rooting rate, 100-200mg L-1Low concentration processing is conducive to improving rooting rate and quality of rooting, but because its is expensive and deposits
In medicament residue, it is unsuitable for wideling popularize in agricultural especially ecological agriculture production.Up to the present, the also non-pin of prior art
The problem of being difficult to take root with Paeonia suffruticosa seed to oil proposes easy and effective and safe processing method.Therefore research promotes oil to use tree peony
The new technology taken root, has realistic meaning for oil with the further investigation and industrialization development of tree peony.
By retrieving domestic and international prior art, not yet find to effectively improve with nutrient interaction by temperature control at present
The simple and easy method of Paeonia suffruticosa seed rooting rate and quality of rooting.
The content of the invention
In view of the deficiencies in the prior art, it is an object of the invention to provide a kind of method for promoting Paeonia suffruticosa seed to take root, with
The with high costs and medicament residue problem for overcoming chemical pesticide or hormone method to exist, so that filling up simplified method improves Paeonia suffruticosa seed life
The technological gap of root rate and quality of rooting.
In order to realize the purpose of the present invention, inventor is finally obtained as follows by lot of experiments research and persistent exploration
Technical scheme：A kind of method for promoting Paeonia suffruticosa seed to take root, it is characterised in that this method comprises the following steps：
(1) 3~7mM CaCl will be put into after intact Paeonia suffruticosa seed cleaning2More than 48h, every 12~24h are soaked in solution
Change once fresh CaCl2Solution；
(2) after immersion terminates, the seed of floating is removed, collecting the seed sunk is used for agricultural production；
(3) seed of collection is placed in and be covered with the flat board of one layer of plastic paper, prevent from oozing under moisture, individual layer tiling takes doctor
With gauze, by gauze 3~7mM CaCl2Solution soaks, and holds not drip with hand and is advisable, and is covered on the seed of tiling, dark
20~30d is cultivated, cultivation temperature is 16~18 DEG C, and the temperature coordinates CaCl2Processing can break the dormancy of seed hypocotyl, cultivate
The CaCl that period passes through 3~7mM of supplement2Solution keeps gauze moistening.
It is further preferred that promoting the method that Paeonia suffruticosa seed is taken root as described above, wherein in step (1) and step (3)
CaCl2The concentration of solution is 3mM.
It is further preferred that promoting the method that Paeonia suffruticosa seed is taken root as described above, Paeonia suffruticosa seed soaks wherein in step (1)
Time is 48~64h.
It is further preferred that promoting the method that Paeonia suffruticosa seed is taken root as described above, the wherein hospital gauze in step (3) is
8 layers.
It is further preferred that promoting the method that Paeonia suffruticosa seed is taken root as described above, the wherein cultivation temperature in step (3) is
17℃。
It is further preferred that promoting the method that Paeonia suffruticosa seed is taken root as described above, wherein described Paeonia suffruticosa seed is selected from resistance to
Damp and hot is ' Feng Dan is white ' and low temperature resistant arid ' purple plague purpura '.
Compared with prior art, the present invention creatively utilizes the CaCl of certain concentration2Proposed with the synergy of temperature
A kind of method for promoting Paeonia suffruticosa seed to take root, the invention has following progressive and beneficial effect：
(1) broken and be only capable of by chemical pesticide or hormone such as GA3Method to improve tree peony rooting rate, has prevented potential
Chemical agent remain harm to plant strain growth, the plantation popularization and feature that can effectively facilitate ecosystem organic farm products are produced
The research and development of product.
(2) the single seed taken root, and the damage in separation process to root are easily separated from colony of taking root
, seldom there is undesirable root of being completely cured in far smaller than traditional sand storage method, and is conducive to the field seedling raising in later stage, its application value compared with
Greatly.
Brief description of the drawings
Fig. 1 is the situation of taking root of Paeonia suffruticosa seed under different calcium concentrations.A-C is respectively ' Feng Dan is white ' seed 0.1,3,15mM
CaCl2Colony under processing takes root situation；Respectively single ' Feng Dan the is white ' seeds of D-F are 0.1,3,15mM CaCl2Under processing
Take root situation；Respectively single ' purple plague purpura ' seeds of G-I are 0.1,3,15mM CaCl2Situation of taking root under processing.
Fig. 2 is the influence of different calcium concentrations and temperature combination to Paeonia suffruticosa seed rooting rate.
Fig. 3 is influence of the different calcium concentrations to ' Feng Dan is white ' seed main root length, lateral root number and lateral root length at 17 DEG C.
Fig. 4 is influence of the different calcium concentrations to ' purple plague purpura ' seed main root length, lateral root number and lateral root length at 17 DEG C.
Embodiment
Be present physiological dormancy in Paeonia suffruticosa seed, take root more difficult, many studies have shown that can break plumular axis by hormonal action
Dormancy, while by sand storage to improve Paeonia suffruticosa seed rooting rate, but this method can residual harmful chemicals in seed body
Matter, is unfavorable for agricultural extension.And the present invention breaks big while seed dormancy by temperature control and the synergy of calcium chloride
The big rooting rate and quality of rooting for improving seed, can not only reduce input cost, and whole method is easy to operate, efficiency
Height, there is stronger application value.The following examples are only made furtherly to technical scheme and technique effect
It is bright, without limiting the present invention in any way.
Embodiment 1：The rooting treatment method of Paeonia suffruticosa seed
(1) need to carry out Paeonia suffruticosa seed primary election before rooting treatment, technical points are to choose the impurity in seed,
And remove damaged or mouldy seed, and cleaned 3 times with clear water；
(2) Paeonia suffruticosa seed cleaned is put into 3mM CaCl248h is soaked in solution, changes once fresh per 24h
CaCl2Solution；
(3) by seed in 3mM CaCl2Soaked in solution after 48h, remove the seed of floating, collecting the seed sunk is used for
Agricultural production；
(4) seed of collection is placed on flat board, layer overlay plastic paper is prevented from oozing under moisture, and seed individual layer is tiled,
8 layers of hospital gauze are taken, by gauze 3mM CaCl2Solution soaks, and holds not drip with hand and is advisable, and is covered on the seed of tiling,
20~30d of dark culturing in 17 DEG C of greenhouses is placed in, a 3mM CaCl is supplemented per 7d2Solution, with maintain seed take root needed for
Humidity.
Embodiment 2：The experimental design of the research of taking root of Paeonia suffruticosa seed
This research is with the different kind of 2 resistances：It is ' Feng Dan is white ' of wet-heat resisting and low temperature resistant arid ' purple plague purpura ', it is oil
The kind promoted with tree peony；The concentration of Calcium treatment is respectively 0.1,3 and 15mM CaCl2；Temperature Treatment is respectively 7 DEG C and 17 DEG C.
Using the test procedure of embodiment 1, result of the test is as follows：
(1) under different calcium concentration conditions Paeonia suffruticosa seed situation of taking root
At 17 DEG C, there is difference in same kind its situation of taking root under the conditions of different disposal.As shown in figs. 1 a-c, pass through
0.1mM CaCl2' Feng Dan is white ' colony seed situation of taking root is poor, and easily infects fungi.The single tree peony kind of different cultivars
Son shows identical trend under identical treatment conditions, as shown in Fig. 1 D-I, with CaCl2Concentration (0.1~15mM)
Under increase, lateral root number showed increased, and same calcium treatment conditions, the situation of taking root of ' Feng Dan is white ' is more managed compared to ' purple plague purpura '
Think.
(2) different calcium concentrations and temperature combine the influence to Paeonia suffruticosa seed rooting rate
Fig. 2 is mainly the relation (note presented between different calcium concentrations and temperature combination and Paeonia suffruticosa seed rooting rate：No matter which kind of
Calcium concentration, ' purple plague purpura ' does not take root at 7 DEG C, and rooting rate is " 0 ", does not show in figure), for temperature, ' Feng Dan is white ' and ' purple
Spot ' most suitable temperature of taking root be 17 DEG C, most suitable calcium concentration at this temperature is 3mM CaCl2, respectively reach
98.9% and 73.9%.
(3) influence of the different calcium concentrations to Paeonia suffruticosa seed quality of rooting
Further influence of the different calcium concentrations to Paeonia suffruticosa seed quality of rooting at 17 DEG C is studied, as a result such as Fig. 3, figure
Shown in 4, ' Feng Dan is white ' and ' purple plague purpura ' Root Morphology under different calcium concentrations show identical variation tendency (respectively such as Fig. 3 and
4), for example：3mM CaCl2The elongation of main root can be effectively induced, the main root length of ' Feng Dan is white ' and ' purple plague purpura ' respectively reaches 18.06
And 18.40cm；15mM CaCl2Lateral root number point on the formation of lateral root, ' Feng Dan is white ' and ' purple plague purpura ' every main root can effectively be induced
7 and 13 are not reached；Influence of the calcium concentration to lateral root length has larger varietal difference, wherein ' Feng Dan is white ' is in 3mM CaCl2
Maximum lateral root long 4.72cm is reached during processing, ' purple plague purpura ' is in 15mM CaCl2Maximum lateral root long 4.14cm is reached during processing.It can be seen that,
17 DEG C, 3mM CaCl2The rooting rate of Paeonia suffruticosa seed can be effectively improved, while tree peony can be induced to form the root types of many fibrous roots, so that
Quality of rooting is effectively improved, is the optimum combination needed for Paeonia suffruticosa seed is taken root.
Claims (6)
1. a kind of method for promoting Paeonia suffruticosa seed to take root, it is characterised in that this method comprises the following steps：
(1) 3~7mM CaCl will be put into after intact Paeonia suffruticosa seed cleaning2More than 48h is soaked in solution, every 12~24h changes one
Secondary fresh CaCl2Solution；
(2) after immersion terminates, the seed of floating is removed, the seed for sinking to solution bottom is collected；
(3) seed of collection is placed in and is covered with the flat board of one layer of plastic paper, individual layer tiling takes hospital gauze, by gauze with 3~
7mM CaCl2Solution soaks, and holds not drip with hand and is advisable, and is covered on the seed of tiling, 20~30d of dark culturing, culture temperature
Spend for 16~18 DEG C, pass through 3~7mM of supplement CaCl during culture2Solution keeps gauze moistening.
2. the method for promoting Paeonia suffruticosa seed to take root according to claim 1, it is characterised in that in step (1) and step (3)
CaCl2The concentration of solution is 3mM.
3. the method for promoting Paeonia suffruticosa seed to take root according to claim 1, it is characterised in that Paeonia suffruticosa seed soaks in step (1)
The bubble time is 48~64h.
4. the method for promoting Paeonia suffruticosa seed to take root according to claim 1, it is characterised in that the hospital gauze in step (3)
For 8 layers.
5. the method for promoting Paeonia suffruticosa seed to take root according to claim 1, it is characterised in that the cultivation temperature in step (3)
For 17 DEG C.
6. according to any one of the claim 1-5 methods for promoting Paeonia suffruticosa seed to take root, it is characterised in that described tree peony kind
Son is ' Feng Dan is white ' and low temperature resistant arid ' purple plague purpura ' selected from wet-heat resisting.
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| CN201710222817.7A CN106961873A (en) | 2017-04-07 | 2017-04-07 | A kind of method for promoting Paeonia suffruticosa seed to take root |
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| CN201710222817.7A CN106961873A (en) | 2017-04-07 | 2017-04-07 | A kind of method for promoting Paeonia suffruticosa seed to take root |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112088777A (en) * | 2020-08-28 | 2020-12-18 | 上海辰山植物园 | In-vitro rooting method for peony tissue culture seedlings |
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|---|---|---|---|---|
| EP0605721A1 (en) * | 1991-08-20 | 1994-07-13 | Kirin Beer Kabushiki Kaisha | Self-dehiscent artificial seed |
| CN104206417A (en) * | 2013-06-04 | 2014-12-17 | 中国科学院上海生命科学研究院 | Method for promoting paeonia suffruticosa seedling growth and application thereof |
| CN104770103A (en) * | 2015-04-15 | 2015-07-15 | 芜湖市丹洋现代农业科技发展有限公司 | Seedling raising method for oil-used peony seeds |
| CN105766132A (en) * | 2016-03-21 | 2016-07-20 | 东北林业大学 | Method for breaking secondary dormancy of morus alba seeds |
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2017
- 2017-04-07 CN CN201710222817.7A patent/CN106961873A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0605721A1 (en) * | 1991-08-20 | 1994-07-13 | Kirin Beer Kabushiki Kaisha | Self-dehiscent artificial seed |
| CN104206417A (en) * | 2013-06-04 | 2014-12-17 | 中国科学院上海生命科学研究院 | Method for promoting paeonia suffruticosa seedling growth and application thereof |
| CN104770103A (en) * | 2015-04-15 | 2015-07-15 | 芜湖市丹洋现代农业科技发展有限公司 | Seedling raising method for oil-used peony seeds |
| CN105766132A (en) * | 2016-03-21 | 2016-07-20 | 东北林业大学 | Method for breaking secondary dormancy of morus alba seeds |
Non-Patent Citations (4)
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| 中国农业科学院农业气象研究室主编: "《北方抗旱技术》", 31 May 1980, 农业出版社 * |
| 贾了然 等: "《山区玉米栽培技术》", 31 January 1998, 中原农民出版社 * |
| 韩芸等: "硫酸锌、氯化钙溶液浸种对玉米种子萌发的影响 ", 《黑龙江八一农垦大学学报》 * |
| 韩芸等: "硫酸锌、氯化钙溶液浸种对玉米种子萌发的影响", 《黑龙江八一农垦大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112088777A (en) * | 2020-08-28 | 2020-12-18 | 上海辰山植物园 | In-vitro rooting method for peony tissue culture seedlings |
| CN112088777B (en) * | 2020-08-28 | 2022-02-22 | 上海辰山植物园 | In-vitro rooting method for peony tissue culture seedlings |
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Application publication date: 20170721 |