CN104206417A - Method for promoting paeonia suffruticosa seedling growth and application thereof - Google Patents
Method for promoting paeonia suffruticosa seedling growth and application thereof Download PDFInfo
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- CN104206417A CN104206417A CN201310221153.4A CN201310221153A CN104206417A CN 104206417 A CN104206417 A CN 104206417A CN 201310221153 A CN201310221153 A CN 201310221153A CN 104206417 A CN104206417 A CN 104206417A
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Abstract
The invention relates to a method for promoting paeonia suffruticosa seedling growth and application thereof. Specifically, the invention provides a paeonia suffruticosa seedling treatment fluid, which comprises: a paeonia suffruticosa seedling growth active agent (gibberellin and/or 6-benzyladenine) and a surfactant, and also can include an agronomically acceptable soluble calcium salt. The growth active agent can break epicotyl dormancy of paeonia suffruticosa seeds, improve the germination rate/germination potential of paeonia suffruticosa seeds, accelerate the paeonia suffruticosa seed growth process, and promote seedling emergency or flowering of paeonia suffruticosa seed seedlings/tissue culture seedlings. The surfactant can make active substances fully enter cells. And the soluble calcium salt is closely related to multiple physiological functions of cells.
Description
Technical field
The invention belongs to phytobiology field, particularly, the present invention relates to a kind of method and the application thereof that promote tree peony seedling early growth.
Background technology
Tree peony (Paeonia suffruticosa), the rich and honour flower of another name, ardisia crispa, wooden Chinese herbaceous peony, mouse aunt, deer fragrant-flowered garlic, Luoyang flower, for Paeoniaceae Paeonia woody plant, machaka, have the good reputation of " aromatic ", " king in spending ", first choice of China's national flower.Tree peony not only has higher ornamental value, also possesses good medical value.As far back as Han dynasty, medicinal plant ancient books and records Shennong's Herbal just has tree peony to be tamed the record of being used as medicine.Along with the increase of tree peony market value in afforestation, medicinal material reserve, expedite the emergence of the economic market of tree peony.Expanding propagation coefficient, improves and expands numerous efficiency, carry out the large-scale production of tree peony seedling, for market rapidly and efficiently provides high-quality tree peony nursery stock at different levels, becomes current tree peony and produces the principal concern with research.
Paeonia suffruticosa seed has hibernation feature under field conditions (factors), is in particular in the dormancy of radicle (hypocotyl) and plumule (epicotyl), especially comparatively deep with top-wall effect.Under normal circumstances, Paeonia suffruticosa seed is sprouted naturally because surviving the winter low temperature breaking dormancy needed for two years, and seedling is bloomed needs about 10 years, and time span is long, and production efficiency is too low, and production cost is too high, the expanding economy of serious restriction tree peony.Therefore, rapidly and efficiently abolish top-wall effect characteristic, effectively improve germination rate and the germination vigor of seed, shorten Paeonia suffruticosa seed seedling and to become to take time span, concerning the tree peony of day by day heating up is economical, significant.
But at present this area still lacks and effectively abolishes Paeonia suffruticosa seed top-wall effect, promotes the effective ways of tree peony seedling early growth, therefore in the urgent need to developing corresponding technical method.
Summary of the invention
Object of the present invention is just to provide one and abolishes Paeonia suffruticosa seed top-wall effect, promotes the method for tree peony seedling early growth.
Another object of the present invention is just to provide one and abolishes Paeonia suffruticosa seed top-wall effect, promotes the treatment fluid of tree peony seedling early growth.
A first aspect of the present invention, provides a kind of tree peony seedling treatment fluid, comprising:
The tree peony seedling early growth activating agent of effective dose and surfactant; And described activating agent is gibberellin (GA
3), and/or 6-benzyladenine (6-BA).
In another preference, described treatment fluid also comprises: acceptable soluble calcium salt on agronomy.
In another preference, in described treatment fluid, gibberellin (GA
3) content be 5-10000mg/L.
In another preference, in described treatment fluid, gibberellin (GA
3) content be 10-10000mg/L, preferably, in described treatment fluid, gibberellin (GA
3) content be 100-1000mg/L.
In another preference, described in described treatment fluid, gibberellin (GA
3) content be 5-1000mg/L.
In another preference, in described treatment fluid, the content of 6-benzyladenine (6-BA) is 0.1-100mg/L.
In another preference, in described treatment fluid, the content of 6-benzyladenine (6-BA) is 0.2-10mg/L, preferably, is 0.2-5mg/L, more preferably, is 0.5-2mg/L.
In another preference, in described treatment fluid, the content of 6-benzyladenine (6-BA) is 0.5-10mg/L.
In another preference, described surfactant is optionally from lower group or its combination:
Anion surfactant (preferred stearic acid or neopelex);
Cationic surfactant (preferred quaternary ammonium compound);
Zwitterionic surfactant (preferably lecithin, amino acid pattern or betain);
Non-ionic surface active agent (preferred fat acid glyceride, fatty acid sorb smooth, tween);
In another preference, described surfactant is Tween-20 or Tween-80.
In another preference, in described treatment fluid, surface-active contents is 1-1000 μ l/L, preferably, is 1-500 μ l/L, more preferably, is 1-150 μ l/L.
In another preference, in described treatment fluid, surfactant is Tween-20, and its content is 1-1000 μ l/L.
In another preference, the content of surface active agent tween-20 is 1-100 μ l/L, is preferably 10-100 μ l/L.
In another preference, on described agronomy, acceptable soluble calcium salt is selected from lower group: Ca(NO
3)
2, Ca(Cl)
2, Ca (H
2pO
4)
2.
In another preference, in described treatment fluid, on described agronomy, the content of acceptable soluble calcium salt is the preferred 1-5mg/L of 0.5-50mg/L().
In another preference, described treatment fluid is the optional treatment fluid from (a)-(c):
(a) 1000mg/L gibberellin (GA
3), 100 μ l/L Tween-20s;
(b) 100mg/L gibberellin (GA
3), 1mg/L6-benzyladenine (6-BA), 5mg/L Ca (NO
3)
2, 10 μ l/L Tween-20s;
(c) 10mg/L gibberellin (GA
3), 0.5mg/L6-benzyladenine (6-BA), 1mg/L Ca (NO
3)
2, 2 μ l/L Tween-20s.
A second aspect of the present invention, provides the purposes for the treatment of fluid described in first aspect, and described treatment fluid has optionally from purposes or its combination of lower group:
(1) the epicotylar dormancy of Paeonia suffruticosa seed is broken;
(2) Paeonia suffruticosa seed germination rate/germination vigor is improved;
(3) Paeonia suffruticosa seed growth course is accelerated;
(4) Paeonia suffruticosa seed seedling/plantlet in vitro seedling is promoted; Or
(5) promote that Paeonia suffruticosa seed seedling/plantlet in vitro becomes flower.
A third aspect of the present invention, provides a kind of method promoting tree peony seedling early growth, comprises step: with the treatment fluid process Paeonia suffruticosa seed described in first aspect or tree peony seedling.
In another preference, described method comprises the treatment fluid process Paeonia suffruticosa seed culture adopted described in first aspect, and described culture is river sand, vegetable garden soil, WPM minimal medium.
In another preference, described method comprises step:
(1) with treatment fluid process Paeonia suffruticosa seed, and described treatment fluid comprises the gibberellin (GA of 1000mg/L
3), and the Tween-20 of 100 μ l/L;
(2) seed that step (1) process obtains is embedded in sand, and described sand is through comprising the gibberellin (GA of 100mg/L
3), the 6-benzyladenine (6-BA) of 1mg/L, the Ca(NO of 5mg/L
3)
2, and the treatment fluid process of Tween-20 of 10 μ l/L, make seed germination, obtain the blade sprouted; With
(3) with comprising 10mg/L gibberellin (GA
3), 0.5mg/L6-benzyladenine (6-BA), 1mg/L Ca(NO
3)
2, and the blade of sprouting that obtains of the treatment fluid treatment step (2) of 2 μ l/L Tween-20s, thus obtain the peony plant with stem.
In another preference, described method comprises step:
A () is with treatment fluid process Paeonia suffruticosa seed, and described treatment fluid comprises the gibberellin (GA of 1000mg/L
3), and the Tween-20 of 100 μ l/L;
The kind shell of b seed that () removal step (a) obtains, and with comprising the gibberellin (GA of 100mg/L
3), the 6-benzyladenine (6-BA) of 1mg/L, the Ca(NO of 5mg/L
3)
2, and the treatment fluid process of Tween-20 of 10 μ l/L, obtain the seed of the band endosperm after process;
C () obtains embryo from the seed of the band endosperm of step (b), be inoculated in medium, obtains the peony plant with stem.
Should be understood that within the scope of the present invention, above-mentioned each technical characteristic of the present invention and can combining mutually between specifically described each technical characteristic in below (eg embodiment), thus form new or preferred technical scheme.As space is limited, tiredly no longer one by one to state at this.
Accompanying drawing explanation
Following accompanying drawing for illustration of specific embodiment of the invention scheme, and is not used in the scope of the invention limiting and defined by claims.
Fig. 1 shows acceleration Paeonia suffruticosa seed seedling production process result; Figure 1A (contrast): naturally sprout the tree peony seedling of 25 months, only has petiole and a blade, and epicotyl is still in resting state; Figure 1B: the tree peony seedling sprouting June after process, tool petiole, blade and obvious stem, top-wall effect is broken.
Fig. 2 shows acceleration tree peony plantlet in vitro production process result; Fig. 2 A(contrasts): the cultured in vitro tree peony embryo of 20 weeks, only have a true leaf, epicotyl is still in resting state; The cultured in vitro tree peony bud seedling of 6 weeks after Fig. 2 B: half amount process, tool true leaf, petiole, blade and obvious stem, top-wall effect is broken.Fig. 2 C: the cultured in vitro tree peony bud seedling of 6 weeks after full dose process, tool true leaf, petiole, blade and obvious stem, top-wall effect is broken.
Embodiment
The present inventor, through extensive and deep research, obtains one first and abolishes Paeonia suffruticosa seed top-wall effect, promote the treatment fluid of tree peony seedling early growth, and promote the method for tree peony seedling early growth.Particularly, described treatment fluid of the present invention comprises: tree peony seedling early growth activating agent (gibberellin and/or 6-benzyladenine) and surfactant, can also comprise acceptable soluble calcium salt on agronomy.Described growth activity agent can break the epicotylar dormancy of Paeonia suffruticosa seed, improves Paeonia suffruticosa seed germination rate/germination vigor, accelerates Paeonia suffruticosa seed growth course, promotes Paeonia suffruticosa seed seedling/plantlet in vitro seedling or becomes flower; Described surfactant can make active substance fully enter in cell; Described soluble calcium salt and the different physiological roles of cell are closely related.Complete the present invention on this basis.
Term
Tree peony
As used herein, term " tree peony " refers to that one belongs to Paeoniaceae (Paeoniaceae), Paeonia (Paeonia), the plant of tree peony kind (P.suffruticosa).
Tree peony of the present invention includes, but is not limited to: Chinese herbaceous peony.
Tree peony seedling
As used herein, term " tree peony seedling " comprising: Paeonia suffruticosa seed, tree peony seedling, tree peony embryo, emerge after containing the seedling of obvious stem.
As used herein, term " Paeonia suffruticosa seed " includes, but is not limited to: tree peony fresh seeds, through the tree peony aged seed of low-temperature storage, comprises kind of shell or removes the Paeonia suffruticosa seed of planting shell.
As used herein, term " tree peony seedling " includes, but is not limited to: the tree peony seedling with petiole and blade.
Tree peony seedling treatment fluid
The invention provides a kind of tree peony seedling treatment fluid, mainly comprise: the tree peony seedling early growth activating agent of (1) effective dose, and described activating agent is gibberellin (GA
3), and/or 6-benzyladenine (6-BA); And the surfactant of (2) effective dose; Preferably, described treatment fluid can also comprise: acceptable soluble calcium salt on agronomy.
As used herein, term " effective dose " or " effective dose " refer to can to tree peony seedling produce function or activity and can the amount that accepts by tree peony seedling.
Described treatment fluid of the present invention may be used for: (1) breaks the epicotylar dormancy of Paeonia suffruticosa seed; (2) Paeonia suffruticosa seed germination rate/germination vigor is improved; (3) Paeonia suffruticosa seed growth course is accelerated; (4) Paeonia suffruticosa seed seedling/plantlet in vitro seedling is promoted; Or (5) promote that Paeonia suffruticosa seed seedling/plantlet in vitro becomes flower.
Gibberellin
As used herein, term " gibberellin " can exchange with " GA " and use.
Gibberellin is the plant hormone that a class extensively exists, and chemical constitution more complicated belongs to Diterpenes acid, is derived and obtain by Fourth Ring skeleton.Gibberellin all contains gibberellane skeleton.In higher plant, the nearest precursor of gibberellin is commonly considered as kaurene.The basic structure of gibberellin is gibberellane, has 4 rings.On gibberellane, because double bond, hydroxy number are different with position, define various gibberellin, known gibberellin class has 38 kinds at least.Different gibberellin biological activity is different, and the activity of gibberellic acid (GA3) is the highest.Active high compound must have a red mould loop systems (ring ABCD), C-7 has carboxyl, A ring has a lactonic ring.
Gibberellin is widely used in agricultural production at present, as improve vintage, or in breeding of hybrid rice florescence alternation to make Parent flower synchronization etc.; Significant effect of increasing production is had to paddy rice, cotton, vegetables, melon and fruit, green manure etc.
Those of ordinary skill in the art can use conventional method to obtain various gibberellin, buys or produce by conventional method as market.A kind of routine produces the method for gibberellin for extract with methyl alcohol.Different gibberellin can separate by various chromatographic technique.
In treatment fluid of the present invention, gibberellin (GA
3) content be 10-10000mg/L, preferred 100-1000mg/L.
6-benzyladenine
As used herein, term " 6-benzyladenine ", " 6-benzyladenine ", " 6-benzyl adenine ", " 6-BA " etc. all can use with exchange.
6-benzyladenine is a kind of broad spectrum activity plant growth regulator, plant cell growth can be promoted, suppress the decomposition of plant chlorophyll, nucleic acid, protein, improve amino acid whose content, Delaying Leaf-Senescence, by amino acid, growth hormone, mineral salt etc. to multiple usefulness such as treatment sites allocation and transportation, be widely used in each stage from germinateing to gathering in the crops of agricultural, fruit tree and horticultural crop.
Those of ordinary skill in the art can use conventional method to obtain 6-benzyladenine, buys or produce by conventional method as market.
In treatment fluid of the present invention, the content of preferred 6-benzyladenine is 0.1-100mg/L; Preferred 0.5-10mg/L.
Surfactant
Treatment fluid of the present invention also comprises surfactant.Also can the material of remarkable reduction table (boundary) surface tension when very low concentrations even if surfactant is a class.Can significantly change table (boundary) surface properties or the material of character that is related to this, that derive from thus as long as to it is generally acknowledged at present under low concentration, surfactant category can be incorporated into.No matter which kind of surfactant, its molecular structure is formed by two parts: one end of molecule is the hydrophobic group (lipophilic group) of non-pole oleophylic; The other end of molecule is the hydrophilic group (lipophobic group or hydrophilic head) of polar hydrophilic.Two class formations and the diametrically opposite molecular fragment of performance or group divide the two ends that are in same molecule and are connected with chemical bond, define a kind of structure of asymmetric, polarity, thus imparting such particular molecule not only hydrophilic, but also oleophylic, is not just again the characteristic of overall hydrophilic or oleophylic.This peculiar structure of surfactant is referred to as " amphiphilic structure " (amphiphilic structure) usually, and thus surfactant molecule is also often referred to as " parents' molecule ".
In the present invention, described surfactant can optionally combine from lower group or its:
Anion surfactant (preferred stearic acid or neopelex);
Cationic surfactant (preferred quaternary ammonium compound);
Zwitterionic surfactant (preferably lecithin, amino acid pattern or betain);
Non-ionic surface active agent (preferred fat acid glyceride, fatty acid sorb smooth, tween);
In another preference, described surfactant is Tween-20 or Tween-80; Its content is 1-1000 μ l/L; Be preferably 10-100 μ l/L.
Soluble calcium salt
In treatment fluid of the present invention, also comprise acceptable soluble calcium salt on agronomy, described calcium salt is selected from lower group: Ca(NO
3)
2, CaCl
2, Ca (H
2pO
4)
2deng.
On described agronomy, the content of acceptable soluble calcium salt is the preferred 1-5mg/L of 0.5-50mg/L().
As used herein, the composition of " acceptable on agronomy " is applicable to tree peony seedling and without excessive bad side reaction, namely has the material of rational benefit/risk ratio.
Application
The invention provides a kind of method promoting tree peony seedling early growth, comprise step: with treatment fluid process Paeonia suffruticosa seed of the present invention or tree peony seedling.
In a preference of the present invention, described method specified place, comprises step: (1) comprises the gibberellin (GA of 1000mg/L with treatment fluid process Paeonia suffruticosa seed, described treatment fluid
3), and the Tween-20 of 100 μ l/L; (2) seed that step (1) process obtains is embedded in sand, and described sand is through comprising the gibberellin (GA of 100mg/L
3), the 6-benzyladenine (6-BA) of 1mg/L, the Ca(NO of 5mg/L
3)
2, and the treatment fluid process of Tween-20 of 10 μ l/L, make seed germination, obtain the blade sprouted; (3) with comprising 10mg/L gibberellin (GA
3), 0.5mg/L6-benzyladenine (6-BA), 1mg/L Ca(NO
3)
2, and the blade of sprouting that obtains of the treatment fluid treatment step (2) of 2 μ l/L Tween-20s, thus obtain the peony plant with stem.
In another preference of the present invention, described method comprises step: (a) is with treatment fluid process Paeonia suffruticosa seed, and described treatment fluid comprises the gibberellin (GA of 1000mg/L
3), and the Tween-20 of 100 μ l/L; The kind shell of b seed that () removal step (a) obtains, and with comprising the gibberellin (GA of 100mg/L
3), the 6-benzyladenine (6-BA) of 1mg/L, the Ca(NO of 5mg/L
3)
2, and the treatment fluid process of Tween-20 of 10 μ l/L, obtain the seed of the band endosperm after process; C () obtains embryo from the seed of the band endosperm of step (b), be inoculated in medium, obtains the peony plant with stem.
The above-mentioned feature that the present invention mentions, or the feature that embodiment is mentioned can be combined.All features that this case specification discloses can with any composition forms and use, each feature disclosed in specification, anyly can be provided identical, alternative characteristics that is impartial or similar object replaces.Therefore apart from special instruction, the feature disclosed is only general example that is impartial or similar features.
Major advantage of the present invention is as follows:
(1) take Paeonia suffruticosa seed as starting material, through a series of process for the treatment of fluid of the present invention, can rapidly and efficiently break Paeonia suffruticosa seed dormancy, greatly shorten Paeonia suffruticosa seed seedling and become flower process with plantlet in vitro seedling;
(2) the phenotype uniformity of process gained peony plant of the present invention and natural germinated plantlet is high, and genetic stability is good, and plantlet in vitro production process accelerates to make the effective character of external source genes of interest import this precious species of tree peony becomes possibility;
(3) the inventive method is tested in triplicate, and the strain of accumulative acquisition Paeonia suffruticosa seed seedling 185, plantlet in vitro 35 strain, plant phenotype and parent's seedling phenotype uniformity of the same race in two kinds of sources are high.
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usual conveniently condition is as people such as Sambrook, molecular cloning: laboratory manual (New York:Cold Spring Harbor Laboratory Press, 1989) condition described in, or according to the condition that manufacturer advises.Unless otherwise indicated, otherwise percentage and number calculate by weight.
Unless otherwise defined, all specialties used in literary composition and scientific words and one skilled in the art the meaning be familiar with identical.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The use that better implementation method described in literary composition and material only present a demonstration.
Embodiment 1
Accelerate Paeonia suffruticosa seed seedling production process
1. choose full Paeonia suffruticosa seed, with the treatment fluid I [gibberellin (GA of 1000mg/L
3)+100ul/L soil temperature (T20) solution] soak 24h, abandon liquid, son of reserving seed for planting, Warm Wash 3 times, air-dry, be placed in 4 DEG C or-20 DEG C of refrigerators with for subsequent use.
2. get clean river sand some, 121 DEG C of high-temperature sterilization 1h, are cooled to normal temperature, with treatment fluid II [100mg/L GA
3+ 1mg/L6-BA+5mg/L Ca (NO
3)
2+ 10ul/L T20] soak into river sand, buried by the Paeonia suffruticosa seed of step (1) in above-mentioned wet sand, the lower 4 DEG C of storages of dark condition 2 months, existing root, begins to sprout.
3. be seeded in the matrix of peat, vegetable garden soil and perlite (3: 6: 1) by simple for the Paeonia suffruticosa seed of step (2) root of hair, 28 ± 2 DEG C, growth 1 month in the booth of natural lighting, begin existing petiole and blade.Buried by the Paeonia suffruticosa seed of step (1) in above-mentioned wet sand, the lower 4 DEG C of storages of dark condition 2 months, existing root, begins to sprout.
4. with treatment fluid III [10mg/L GA
3+ 0.5mg/L6-BA+1mg/L Ca (NO
3)
2+ 2ul/L T20] spray the little seedling leaf that step (3) obtains, 7 days/time, when 3 months, grow up to the tree peony plantlet of the obvious stem of tool, see Figure 1B, Figure 1A is contrast.
5. hypocotyl is cut into the cut-out of 2-4 millimeter with for subsequent use, the Paeonia suffruticosa seed of root of hair is seeded in the matrix of peat, vegetable garden soil and perlite (3: 6: 1) simplely, and 28 ± 2 DEG C, growth 1 month in the booth of natural lighting, begin existing petiole and blade.
If only use 6-BA process, weak effect, and the present embodiment result shows that above-mentioned formula has effect that live body breaks Paeonia suffruticosa seed dormancy.Result need under showing nature 2 years ability seedling (see obvious stem) Paeonia suffruticosa seed, under the manual belle treatment conditions of the present embodiment, get final product seedling (see obvious stem) in the short time.Compare, there is effect that live body breaks Paeonia suffruticosa seed dormancy.
Embodiment 2
Accelerate tree peony plantlet in vitro production process
1. choose full Paeonia suffruticosa seed, with the treatment fluid I [gibberellin (GA of 1000mg/L
3)+100ul/L soil temperature (T20) solution] soak 24h, abandon liquid, son of reserving seed for planting, Warm Wash 3 times, air-dry, be placed in 4 DEG C or-20 DEG C of refrigerators with for subsequent use.
2. the kind shell of Paeonia suffruticosa seed that obtains of strip step (1), with treatment fluid II [100mg/L GA
3+ 1mg/L6-BA+5mg/L Ca (NO
3)
2+ 10ul/L T20] soak 24h, abandon liquid, the seed of collecting belt endosperm, filter paper blots surface moisture.
3. band albuminous seed running water step (2) obtained is cleaned, blot surface water drops, with the ethanol disinfection 30-90 second of 70% on superclean bench, the mercuric chloride of 0.1% soaks 5-8 minute, aseptic water washing 5-8 time, aseptic filter paper blots sterilizable material surface water drops, strips out embryo, is inoculated in WPM minimal medium (collective media of woody plant cultured in vitro) and adds 5mg/L GA3+0.5mg/L BA+1mg/LCa (NO
3)
2medium in, under illumination, the condition of culture of 25 ± 2 DEG C, 2 weeks saw lobus cardiacus, and 4 weeks saw obvious stem, and see that Fig. 2 B and Fig. 2 C, Fig. 2 B is half amount treatment effect, Fig. 2 C is full dose treatment effect, and Fig. 2 A is contrast, is inoculated in WPM minimal medium.
4. the aseptic bud seedling that step (3) obtains is forwarded in the medium of WPM minimal medium annex 2mg/L abscisic acid ABA+1mg/L paclobutrazol, cultivated for 2 weeks, can culture of rootage be proceeded to, see Fig. 2 C.
Result shows: above-mentioned formula has the effect breaking Paeonia suffruticosa seed dormancy in vitro.Need under nature 2 years ability seedling (see obvious stem) Paeonia suffruticosa seed, only need within 1 month, to become bud seedling (see obvious stem) under the cultured in vitro and treatment conditions of the present embodiment, through root induction, the soil transplanted seedling of the obvious stem of tool can be obtained in 3 months.
The all documents mentioned in the present invention are quoted as a reference all in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after having read above-mentioned instruction content of the present invention.
Claims (10)
1. a tree peony seedling treatment fluid, is characterized in that, described treatment fluid comprises:
The tree peony seedling early growth activating agent of effective dose and surfactant; And described activating agent is gibberellin (GA
3), and/or 6-benzyladenine (6-BA).
2. treatment fluid as claimed in claim 1, it is characterized in that, described treatment fluid also comprises: acceptable soluble calcium salt on agronomy.
3. the treatment fluid as described in as arbitrary in claim 1-2, is characterized in that, in described treatment fluid, and gibberellin (GA
3) content be 5-10000mg/L.
4. the treatment fluid as described in as arbitrary in claim 1-2, it is characterized in that, in described treatment fluid, the content of 6-benzyladenine (6-BA) is 0.1-100mg/L.
5. the treatment fluid as described in as arbitrary in claim 1-2, is characterized in that, described surfactant is optionally from lower group or its combination:
Anion surfactant (preferred stearic acid or neopelex);
Cationic surfactant (preferred quaternary ammonium compound);
Zwitterionic surfactant (preferably lecithin, amino acid pattern or betain);
Non-ionic surface active agent (preferred fat acid glyceride, fatty acid sorb smooth, tween).
6. treatment fluid as claimed in claim 2, it is characterized in that, on described agronomy, acceptable soluble calcium salt is selected from lower group: Ca(NO
3)
2, Ca(Cl)
2, Ca (H
2pO
4)
2.
7. the purposes for the treatment of fluid described in claim 1, is characterized in that, described treatment fluid has optionally from purposes or its combination of lower group:
(1) the epicotylar dormancy of Paeonia suffruticosa seed is broken;
(2) Paeonia suffruticosa seed germination rate/germination vigor is improved;
(3) Paeonia suffruticosa seed growth course is accelerated;
(4) Paeonia suffruticosa seed seedling/plantlet in vitro seedling is promoted; Or
(5) promote that Paeonia suffruticosa seed seedling/plantlet in vitro becomes flower.
8. promote a method for tree peony seedling early growth, it is characterized in that, comprise step: with treatment fluid process Paeonia suffruticosa seed according to claim 1 or tree peony seedling.
9. method as claimed in claim 8, is characterized in that, comprise step:
(1) with treatment fluid process Paeonia suffruticosa seed, and described treatment fluid comprises the gibberellin (GA of 1000mg/L
3), and the Tween-20 of 100 μ l/L;
(2) seed that step (1) process obtains is embedded in sand, and described sand is through comprising the gibberellin (GA of 100mg/L
3), the 6-benzyladenine (6-BA) of 1mg/L, the Ca(NO of 5mg/L
3)
2, and the treatment fluid process of Tween-20 of 10 μ l/L, make seed germination, obtain the blade sprouted; With
(3) with comprising 10mg/L gibberellin (GA
3), 0.5mg/L6-benzyladenine (6-BA), 1mg/L Ca(NO
3)
2, and the blade of sprouting that obtains of the treatment fluid treatment step (2) of 2 μ l/L Tween-20s, thus obtain the peony plant with stem.
10. method as claimed in claim 8, is characterized in that, comprise step:
A () is with treatment fluid process Paeonia suffruticosa seed, and described treatment fluid comprises the gibberellin (GA of 1000mg/L
3), and the Tween-20 of 100 μ l/L;
The kind shell of b seed that () removal step (a) obtains, and with comprising the gibberellin (GA of 100mg/L
3), the 6-benzyladenine (6-BA) of 1mg/L, the Ca(NO of 5mg/L
3)
2, and the treatment fluid process of Tween-20 of 10 μ l/L, obtain the seed of the band endosperm after process;
C () obtains embryo from the seed of the band endosperm of step (b), be inoculated in medium, obtains the peony plant with stem.
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