CN114403154A

CN114403154A - Plant growth regulator composition and preparation method and application thereof

Info

Publication number: CN114403154A
Application number: CN202210139622.7A
Authority: CN
Inventors: 高俊平; 马男; 周晓锋; 景维坤; 段金辉; 孙小明; 李永红; 周厚高
Original assignee: China Agricultural University
Current assignee: China Agricultural University
Priority date: 2022-02-15
Filing date: 2022-02-15
Publication date: 2022-04-29

Abstract

The present invention relates to a plant growth regulator composition comprising a first component involved in the regulation of the transcriptional level of cell development in response to cell development, a second component inducing a promoter response in response to cell division, and a third component providing a base material for the first component and the second component to be involved in a life activity. A plant growth regulator composition can act on the pedicel or inflorescence tissue of elbow rose, thereby restoring the elbow rose to a normal state with commercial value.

Description

Plant growth regulator composition and preparation method and application thereof

Technical Field

The invention relates to the field of agriculture, in particular to a plant growth regulator composition and a preparation method thereof.

Background

China rose (Rosa hybrida), which is evergreen or semi-evergreen shrub of Rosa of Rosaceae, has many skin thorns, bright and beautiful flower color, large and beautiful flower, and can bloom throughout the year if proper conditions are met, so it is reputed as "queen in flower". The cut flower Chinese rose is the first of four fresh cut flowers in the world, and has important ornamental value and market value in China and even all over the world. According to the statistics of agricultural rural departments of the people's republic of China, the sales volume (726172.2 ten thousand branches) and the sales volume (480650.9 ten thousand yuan) of the cut flowers of China in 2016 account for more than one third of the total sales volume and the sales volume of fresh cut flowers in China. Therefore, it is important to produce cut flower roses of high quality and high yield.

However, in the actual cultivation process, the cut rose is affected by the genetic background of the cut rose or abnormal growth environment caused by improper cultivation management, so that the cut rose is bent. The cut rose with the elbow loses commodity value because the flower stalk is bent if the cut rose is not intervened in the acquired environment. The elbow of the cut flower Chinese rose means that the stem node under the receptacle is bent and thickened, and the implantation position of the sepals is abnormal, so that the flower stem is not perpendicular to the flower bud, and the flower head of the cut flower Chinese rose is bent to a larger extent towards one side instead of the normal flower head of the Chinese rose, thereby forming the 'elbow' phenomenon that the flower bud of the Chinese rose is distorted. According to statistics, the incidence rate of elbows is 10% on average in the production of the cut flower Chinese roses, the cut flower Chinese roses with the elbows cannot be sold as commodities and can only be cut off and destroyed, huge economic losses are caused to flower industries, enterprises and farmers, and the economic loss scale caused each year is up to thousands of yuan.

For the morphological problem of the cut flower rose, the observation of high temperature in the morphological and anatomical observation of the bent stem phenomenon of the cut flower rose by people with even stony stress is one of the causes of the bent stem phenomenon, and the petals of the plant with the bent stem are reduced, the flower head is reduced, and the ovary is flattened. Vascular bundles, phloem and sclerenchyma cells at the bent stem part are all obviously changed and bent towards the falling side of the No. 1 sepal.

At present, an effective solution and a related treatment scheme for elbow treatment of the cut rose are not available. In the prior art, the application range of phytohormones is mostly induced in seeds for promoting growth speed or breaking dormancy, for example, Chinese patent with publication number CN105993938B discloses a method for inducing emergence of seedlings of in vitro pseudo-ginseng dormant buds by gibberellin, which comprises the steps of taking field pseudo-ginseng, cutting and cleaning dormant buds, removing cracked tissues on the surface, soaking the buds for 1-2 min by 70% of ethanol, cleaning the buds by sterile water, soaking the buds for 8-10min by 10% of sodium hypochlorite solution, washing the buds by sterile water, planting the treated dormant buds on an MS culture medium containing gibberellin, performing illumination culture, emerging seedlings within 6-7 days, transferring the buds into an MS culture medium containing cytokinin 6-BA and auxin A, differentiating and rooting the grown pseudo-ginseng seedlings within 3 weeks, and transplanting the seedlings into a field for transplantation. Or Chinese patent with patent number CN102197768B, relating to a method for adjusting the flowering phase of paphiopedilum firmum, and utilizing gibberellin GA with different concentrations₃And 6-BA treating paphiopedilum harderi adult plants respectively, wherein GA is adopted for gibberellin treatment₃ 100mgL^-1，GA₃ 200mgL^-1，GA₃ 400mgL^-1(ii) a The 6-BA treatment adopts 6-BA 100mgL^-1，BA 200mgL^-1，BA 400mgL^-1. The flower organ tissue ripening or anti-aging operation is carried out by the method. The above methods are all the methods for carrying out abnormal growth of plant part tissues in a mode of interfering normal growth by using phytohormones.

The invention develops a reagent with simple operation, quick response and obvious effect, and aims at the abnormal plants caused by phytohormone signal transduction, and adopts phytohormone mediation to ensure normal hormone signal transduction, so that the plants develop normally and meet the requirement of commodity standard.

Moreover, the applicant hereby gives notice that, in the absence of any element of skill in the art, such element is not necessarily in any way exhaustive or that certain features may be combined in a manner consistent with the principles of the present invention.

Disclosure of Invention

In response to the deficiencies of the prior art, the present invention provides a plant growth regulator composition comprising a first component responsive to cell development and involved in the regulation of the transcriptional level of cell development, a second component inducing a promoter response in response to cell division, and a third component providing the basis material required for the first component, the second component, to participate in a vital activity.

In the bent cut flower rose, the abnormal priorities of the morphologies of different tissue parts are different, for example, in the early development stage of a flower bud, the part of the flower stalk close to the flower bud mainly exists in a primary structure which is a part with cell division and differentiation into preferential life activities, so that the flower stalk is bent under the influence of external factors, the cell division speed and degree of two sides are different due to the uneven distribution of hormones on the two sides of the flower stalk, the number of cells on the inner side of the position is obviously less than that on the outer side, and finally the growth of the two sides is uneven to form a bent head. Similarly, curved floral stalk tissue extending in the direction of floral stalk growth away from the buds creates a secondary structure as it requires a stable nutrient transport space and support for the top buds. The secondary structure focuses on the growth and development of cells, forming cells with cell walls and large vacuoles to protect the morphology and nutrient storage capacity of the cells. During the growth and development stage of cells, hormone signaling pathways involved in the regulation of transcriptional levels of cell development and hormone regulation in response to cell development are more active. Therefore, in the bent rose, the secondary structure of its bent side causes a decrease in the state of cell development due to the external cause of its hormonal regulation disorder, a process that requires the participation and maintenance of the second component.

According to a preferred embodiment, the third component is a plant amino acid involved in the vital activities of cell division or growth. Plant amino acids are Essential Amino Acids (EAA). Preferably, the plant amino acids comprise lysine and leucine involved in plant photosynthesis, methionine involved in respiration to increase plant growth rate, and glutamic acid involved in photorespiration nitrogen metabolism and functioning to reduce nitrate content in plants. Preferably, the ratio of methionine, glutamic acid and lysine is 2: 1: 1. preferably, lysine, methionine and glutamic acid are mixed in equal proportion to constitute the third component based on the synergistic effect of elongated cell growth and photorespiration in the secondary structure of the bent rose.

According to a preferred embodiment, the first component is tricyclic adenine which is stable in an acidic environment and is capable of acting on the stem or side branches of a plant. Preferably, the first component is 6-benzylaminoadenine.

According to a preferred embodiment, the second component comprises one or more tetracyclic diterpene compounds and isomers thereof. Preferably, the second component is GA₁、GA₂、GA₃、GA₄、GA₅、GA₆、GA₇、GA₈、GA₉Or GA₁₀One or more of (a). In particular, the second component comprises GA₃。

According to a preferred embodiment, the composition is an alcoholic dispersion of the first, second and third components, wherein the alcoholic dispersion comprises water for reducing the concentration of the alcohol to reduce the corrosiveness of the alcohol to the plant tissue.

According to a preferred embodiment, the alcoholic dispersion comprises at least one ester and a carrier for the ester for stabilizing the organic substance.

According to a preferred embodiment, the first component is present in a higher amount than the third component, and the second component is present in a higher amount than the third component.

A method of preparing a plant growth regulator composition comprising the steps of: adding the second component into a solvent consisting of a low concentration of an alcohol; adding the first component and the third component; and adding a carrier after the mixed solution is clarified, and adjusting the pH of the solution to be weakly acidic to obtain the plant growth regulator composition acting on the elbow rose.

According to a preferred embodiment, a method of preparing a plant growth regulator composition comprises the steps of: adding 0.1mol/L sodium hydroxide into the first component to prepare 1g/L mother liquor; adding absolute ethyl alcohol into the second component, and adding water to prepare 1g/L mother liquor after the second component is dissolved; adding water into the third component to prepare 1g/L mother solution; mixing the heated and dissolved carrier with water, adding the mother liquor of the first component, the mother liquor of the second component and the mother liquor of the third component, adding water to a fixed amount of 1L, and shaking and mixing; and (3) after the mixture is sufficiently and uniformly mixed, adjusting the pH value of the mixed solution to 5-6, and obtaining the plant growth regulator composition acting on the elbow China rose.

According to a preferred embodiment, the concentration of the first component in the mixed solution can be 5 to 20 mg/L. Preferably, the concentration of the first component can be 10 mg/L. More preferably, the first component can be 15mg/L of 6-benzylamino adenine.

According to a preferred embodiment, the concentration of the second component in the mixed solution can be 5 to 20 mg/L. Preferably, the second component consists of 5-20 mg/L of GA₃And (4) forming. More preferably, the second component is 15mg/L GA₃。

According to a preferred embodiment, the second component consists of 10mg/L GA₁、10mg/L GA₂、10mg/L GA₃、10mg/L GA₄、10mg/L GA₅、10mg/L GA₆、10mg/L GA₇、10mg/L GA₈、10mg/L GA₉Or 10mg/L GA₁₀One or more of the above.

According to a preferred embodiment, the second component consists of 15mg/L GA₁、15mg/L GA₂、15mg/L GA₃、15mg/L GA₄、15mg/L GA₅、15mg/L GA₆、15mg/L GA₇、15mg/L GA₈、15mg/L GA₉Or 15mg/L GA₁₀One or more of the above.

According to a preferred embodiment, the second component consists of 20mgL GA₁、20mg/L GA₂、20mg/L GA₃、20mg/L GA₄、20mg/L GA₅、20mg/L GA₆、20mg/L GA₇、20mg/L GA₈、20mg/L GA₉Or 20mg/L GA₁₀One or more of the above.

According to a preferred embodiment, the concentration of the third component in the mixture can be 0.2 to 1 mg/L. Preferably, the third component can be 0.5mg/L of plant amino acids.

According to a preferred embodiment, the pH of the mixed solution is in the range of 3 to 4 or 5 to 6. Preferably, the pH can be 5.7.

A method for solving elbow of cut flower rose comprises applying the above plant growth regulator composition to rose with elbow phenotype. Preferably, the plant growth regulator composition acts on the peduncle part of the rose having an elbow phenotype.

According to a preferred embodiment, a method for solving the problem of the elbow of the cut rose comprises the following steps:

selecting qualified roses with elbow phenotypes; determining the bending part of the rose with the elbow phenotype; the plant growth regulator composition is applied to the curved side of the curved part. Preferably, the curved side is an inner side of a recess where the rose is curved.

Drawings

FIG. 1 is a schematic view of an untreated elbow rose provided by the present invention;

FIG. 2 is a schematic view of a treated elbow rose provided by the present invention;

fig. 3 is a schematic structural view of the apparatus shown in embodiment 6 of the present invention.

Reference numerals

100: a container; 110: a channel; 200: a flexible application assembly; 210: a smearing zone; 220: a non-permeable region; 300: a protruding outer wall; 400: a cavity.

Detailed Description

The following detailed description is made with reference to the accompanying drawings.

Plant hormone is a trace amount of physiologically active organic substance which is induced by a certain signal received by plant cells, metabolized and synthesized in specific tissues of plants, and combined with a specific protein receptor to regulate the growth and development of plants.

Aiming at the elbow phenomenon, the elbow of the rose is found to be related to the distribution of plant phytohormones by analyzing the cell growth change and transcriptome of the elbow rose, so that the problem is solved by smearing an exogenous plant growth regulator.

Preferably, the curved side of a rose bend referred to herein refers to the concave inner side of the curve of the rose bend.

Preferably, the range of bending angles can be 20 ≦ α ≦ 90.

Preferably, when the bending angle of the pedicel portion of the rose is less than 20 °, it can be regarded as a normal rose meeting the demand of commercial value. When the bending angle of the peduncle portion of the rose exceeds 35 °, it is considered as an elbow rose having an elbow appearance and having no commercial value.

Preferably, the bending angle of the pedicel part of the rose having a bending angle exceeding 35 ° after the correction by the formulation and application method referred to in the present application is less than 20 °, the correction effect is regarded as significant.

Example 1

1g/L of 6-benzylamino adenine was added to 1L of 0.1mol/L sodium hydroxide to prepare 1g/L of a mother liquor.

10ml of absolute ethanol dissolved 1g of GA₃Then 990ml of water was added to prepare 1g/L of GA₃The mother liquor of (4).

1g of plant amino acid is dissolved in 1L of water to prepare 1g/L of plant amino acid mother liquor.

The preparation method comprises the following steps: heating and dissolving lanolin 200g/L, and adding 200ml water into a constant volume bottle. Then, 10ml of a mother liquor of 6-benzylaminopurine and 10ml of GA were added in this order₃And 0.5ml of a plant amino acid mother liquor. Adding water to a constant volume of 1000ml, shaking vigorously to mix the mixture evenly, and adjusting the pH value to 5.8. A plant growth regulating composition comprising 10 mg/L6-benzylaminopurine, 10mg/L gibberellin, 0.5mg/L plant amino acids and 200g/L lanolin and using anhydrous ethanol and water as solvents was obtained.

The formulated composition was applied to the elbow rose and the recovery of the elbow rose was observed.

The specific application method comprises the following steps:

selecting a variety of cut flower Chinese rose, namely 'nectarine snow mountain', selecting 30 bent cut flower Chinese roses in a field, wherein the bending angle of each bent bend is more than 20 degrees, uniformly coating the regulator on the bent side of each bent stem by using a cotton swab, and immediately photographing after coating.

After 24 hours, observation and photographing were performed again. The standard with remarkable treatment effect is that the bending angle of the elbow of the Chinese rose is 35-45 degrees (included angle with the vertical direction). Photographs of each applied rose were taken and statistics of the percent significant effect were performed as shown in table 1.

TABLE 1

Treatment of	Number of bends	Number of significant effects	Significant percentage of the effect
				Nectarine snow mountain	30	17	57％

Fig. 1 shows an untreated elbow rose, the degree of bending of the flower bud is large, the rose cannot be normally harvested as a commodity, a cotton swab is used for dipping a small amount of regulator, the regulator is gently and uniformly coated on the inner side of the bent flower stalk, observation and photographing are carried out after 24 hours, the effect of fig. 2 appears when the rose with a remarkable effect is treated, the flower stalk becomes vertical, and the state of a normal plant is recovered.

Example 2

This example was carried out in substantially the same manner as in example 1 except for the following.

The preparation method comprises the following steps: heating and dissolving lanolin 200g/L, and adding 200ml water into a constant volume bottle. Then, 15ml of a mother liquor of 6-benzylaminopurine and 15ml of GA were added in this order₃And 0.5ml of a plant amino acid mother liquor. Adding water to a constant volume of 1000ml, shaking vigorously to mix the mixture evenly, and adjusting the pH value to 5.6. A plant growth regulating composition comprising 15 mg/L6-benzylaminopurine, 15mg/L gibberellin, 0.5mg/L plant amino acids and 200g/L lanolin and using anhydrous ethanol and water as solvents was obtained.

The specific application method comprises the following steps:

selecting a variety of cut flower Chinese rose, namely 'nectarine snow mountain', selecting 30 bent cut flower Chinese roses in a field, uniformly coating the regulator on the bent side of a bent flower stalk by using a cotton swab, and immediately photographing after coating.

After 24 hours, observation and photographing were performed again. Photographs of each applied rose were taken and statistics of the percent significant effect were performed as shown in table 2.

TABLE 2

Treatment of	Number of bends	Number of significant effects	Significant percentage of the effect
				Nectarine snow mountain	30	26	87％

Example 3

This example was carried out in substantially the same manner as in example 1 or example 2 except for the following.

The preparation method comprises the following steps: heating and dissolving lanolin 200g/L, and adding 200ml water into a constant volume bottle. Then 20ml of a mother liquor of 6-benzylamino adenine and 20ml of GA were added in this order₃And 0.5ml of a plant amino acid mother liquor. Adding water to a constant volume of 1000ml, shaking vigorously to mix the mixture evenly, and adjusting the pH value to 6.0. A plant growth regulating composition comprising 20 mg/L6-benzylaminopurine, 20mg/L gibberellin, 0.5mg/L plant amino acids and 200g/L lanolin and using anhydrous ethanol and water as solvents was obtained.

The specific application method comprises the following steps:

After 24 hours, observation and photographing were performed again. Photographs of each applied rose were taken and statistics of the percent significant effect were performed as shown in table 3.

TABLE 3

Treatment of	Number of bends	Number of significant effects	Significant percentage of the effect
				Nectarine snow mountain	30	23	76％

Example 4

This example was carried out in substantially the same manner as in example 1, example 2 or example 3, except as follows.

The invention can be applied to the tissue lateral bending phenomenon caused by the asymmetry of plant cells.

After the cut rose enters the reproductive growth stage, the flower stalks close to the buds are mainly primary structures, and the upright flower stalks for supporting the rose buds can form secondary structures outside the primary structures.

The primary structure contains many new cells that focus on cell division for the relevant functional differentiation of tissues containing the protective, basal and vascular systems.

After the secondary structure is subjected to differentiation of related functional cells, the secondary structure cells are stimulated to grow in a manner of increasing the thickness and hardness of the pedicel, so that the pedicel can support the erection of the buds and can transport nutrition to the buds.

Compared with the normal operation of the cell function at different positions, the structural cells of the elbow Chinese rose stems have abnormal operation states, such as the division reduction of primary structures or the growth reduction of secondary structures. Such abnormal cell growth states are mostly caused by external factors such as light, water shortage or nutrient deprivation. When the problem of elbow of cut rose occurs, the prior art adopts a method of cutting off buds, and the cutting off of buds can reduce the commercial yield of the cut rose.

The plant regulator containing at least two compositions is adopted, and the application of the exogenous compositions stimulates the cell division or growth of the bent part, so that the bent part expands along the growth direction of the pedicel to jack up the elbow, the bilateral symmetrical growth of the cut rose pedicel is ensured, and the ornamental value and the commercial value of the cut rose pedicel can be guaranteed.

The growth of Chinese rose is regulated by administering the composition with different hormone ratios to cells in different growth phases. Specifically, based on the growth differentiation on the time axis, the formed elongated cells with secondary structure and the meristematic cells with primary structure possess different growth priorities. The primary structure is generally located at the top of the embryo, stem end or root end of the plant, which is primarily through cell division to ensure normal functional cell operation of the plant stem or root. The secondary structure is generally located in the middle section of the stalks and roots, connecting with the primary structure. The elongated cells in the secondary structure are generally formed by differentiation of meristematic cells of the primary structure, i.e., the differentiated cells are transformed from preferential division to preferential growth, so that the length and thickness of the plant stem or root are increased. Meanwhile, the cell division life activities in the primary structure are more active than the cell growth life activities, so that the hormone expression related to the cell division regulation and control approach in the primary structure is more active, and the hormone expression related to the cell growth regulation and control approach in the secondary structure is more active. At the bent part of the rose of the elbow, the stem close to the sepal or the bud part of the rose leads to the reduction of the cell number in the elbow process due to the hormone expression loss related to the cell division regulation pathway, and the stem close to the root leads to the reduction of the cells in the elbow process due to the hormone expression loss related to the cell growth regulation pathway. During growth, cells of the primary structure are reduced resulting in the same reduction of cells of the subsequent secondary structure, and cells of the secondary structure are reduced resulting in the total length of the side being less than the other side to curve.

The plant conditioner of the present invention comprises a first composition and a second composition. Wherein the first composition comprises a first component, a second component and a third component, the first component is lower in content than the second component, and the third component is lower than the first component and the second component; the second composition comprises a first component, a second component, and a third component, the first component being present in an amount greater than the second component, and the third component being present in an amount less than the first component and the second component.

According to a preferred embodiment, the first composition comprises 20 mg/L6-benzylamino adenine, 20 mg/L-160 mg/L gibberellin and 0.5mg/L plant amino acid, and 200g/L lanolin is used as a carrier, and absolute ethyl alcohol and distilled water are used as solvents to prepare the composition for regulating plant growth, wherein in order to avoid the inhibition effect of high-concentration gibberellin and 6-benzylamino adenine on plant growth, 25mg gibberellin, 0.5mg plant amino acid and 20mg 6-benzylamino adenine are added into 1000mL of proportioning solution.

According to a preferred embodiment, the second composition comprises 20mg/L to 160mg/L of 6-benzylamino adenine, 20mg/L of gibberellin and 0.5mg/L of plant amino acid, and is prepared into a composition for regulating plant growth by taking 200g/L of lanolin as a carrier and taking absolute ethyl alcohol and distilled water as solvents, wherein 20mg of gibberellin, 0.5mg of plant amino acid and 30mg of 6-benzylamino adenine are added into 1000mL of proportioning solution.

The specific application method comprises the following steps:

the first composition is applied to the curved side of the bent rose pedicel to stimulate the secondary structure of the pedicel at that location for normal cell growth;

the second composition is applied to the curved side of the bent rose pedicel to stimulate the nascent structures of the pedicel at that location to undergo normal cell division with an external source.

The cell growth of the secondary structure is recovered, the cell number of the primary structure is recovered, so that the bent side of the bent part recovers a normal cell tissue structure, and the lengths of two sides of the stalk are similar and upright based on the recovery of the stalk length.

Based on the above composition and application method, the results show that the elbow rose with the bending angle of 35-45 degrees can be restored to normal (the bending angle is lower than 20 degrees) at least on the third day after the application, and the percentage of the rose with the significant effect of the treatment is more than 50 percent.

According to a preferred embodiment, the first composition is applied to the curved side of the pedicel of the bent rose away from the bud, with exogenous stimulation of the secondary structure at that location of the pedicel for normal cell growth; the second composition is applied to the curved side of the pedicel of the bent rose near the bud, and externally stimulates the primary structure of the pedicel at that location for normal cell division.

Example 5

This example was carried out in substantially the same manner as in example 1, example 2, example 3 or example 4, except as follows.

The present invention relates to an administration method and a composition for determining the degree of vital movement of a bent part of a rose based on the degree of bending, in order to regulate abnormal vital movement of the bent part by exogenously administering different compositions, because of two abnormal states, namely, reduction in cell elongation strength and reduction in cell division ability of the inner side of the bent part compared to the outer side.

According to a preferred embodiment, the plant regulator of the invention is capable of exogenously stimulating plant tissue sites of different hormone content states, based on the degree of bending of the bent rose. When the same kind of Chinese rose has an elbow, the Chinese rose with different bending degrees can generate different degrees of disorder of regulation and control channels based on the growth cycles of the secondary structure and the primary structure. The activity of the cell life activity on the bending side is judged by the bending angle of the Chinese rose elbow, and the proportion of the externally applied composition is carried out based on the activity of the cell life activity.

The ratio of the first component and the second component of the first composition can be calculated according to the deficiency condition related to the activity of the regulatory factor of cells on the bending side of the rose when the rose is in the bent state.

Take the angle α of a rose bend as an example. When the bent sides of the roses were detected to have an angle of 25 °, it was known that the hormone deficiency associated with cell growth and division at the bent sides of the bent roses was increased by 5%, based on comparison with the normal growth curve of the roses, particularly the growth curve of the cells of the stalk at the bent sides, to distinguish the regulator activity of the cells at the bent sides of the bent roses and to know that the regulator activity of the cells at the bent sides of the bent roses was decreased by 5%. Based on this, the minimum content of the first component for promoting cell division in the first composition applied to the curved side of the bent rose pedicel was determined to be (1+ 5%) 20mg/L, and the actual ratio of the first component and the second component in the first composition generated based on the requirement that the second component in the first composition is higher than the first component was: each 1000mL of the composition contains 21mg of the first component and 22-160 mg of the second component.

Similarly, the ratio of the first and second components of the second composition can be calculated according to the deletion condition associated with the activity of the regulatory factor of the cells on the bent side of the rose when the rose is in the bent state.

When the bending of the bent rose elbow to an angle of 35 ° was detected, it was known that hormone deficiency associated with cell growth and division at the bent side of the bent rose was increased by 5%, based on comparison with the normal growth curve of the rose, particularly the growth curve of the cells of the stalk at the bent side, to distinguish the regulator activity of the cells at the bent side of the bent rose and to know that the regulator activity of the cells at the bent side of the bent rose was decreased by 5%. Based on this, the minimum content of the second component for promoting cell division in the first composition applied to the curved side of the peduncle of the rose elbow was determined to be (1+ 5%) 20mg/L, and the actual ratio of the first component and the second component in the second composition generated based on the requirement that the first component in the second composition is higher than the second component was: each 1000mL of the composition contains 21mg of the second component and 22-160 mg of the first component.

Example 6

This example was carried out in substantially the same manner as in example 1, example 2, example 3, example 4 and example 5 except for the following.

The method has a judgment method for the application content of the secondary structure and the primary structure.

According to a preferred embodiment, the content of the first component of the secondary and primary structures of normal tissue is a mg/L. The second component content of the secondary structure and the primary structure of the normal tissue structure was bmg/L. Thus, during application, when the angle of curvature of the rose elbow is not greater than α, then the second component in the second composition applied to the curved side of the rose elbow stalk does not exceed b. When the angle of bending of the rose elbow is not more than α, the first component of the first composition applied to the curved side of the rose elbow stalk does not exceed a.

During application, when the bending angle of the rose elbow is greater than alpha, the second composition is applied to the bent side of the rose elbow peduncle in an amount greater than b. When the angle of the elbow of the rose is greater than α, the first component content of the first composition applied to the curved side of the stalk of the elbow rose away from the bud is higher than a. For example, when the elbow angle of China rose is 45 °, the content of the first component of the primary structure of the bent side thereof is measured to be 10mg/cm²The single application amount of the primary structure of the first component to the curved side in the first composition during the application process cannot exceed 10mg/cm²。

The compositions of the present invention can be based on a device that facilitates application to the bends of the elbow rose for site specific application of the elbow rose based on application of different drugs to different sites.

Specifically, the apparatus comprises: a container 100 containing a composition as contemplated by the present invention; a passage 110 communicating with the container 100 for transferring the composition in the container 100, the passage 110 being provided at an axial position of the container 100; a flexible spreading member 200 permeable to the composition disposed at the top of the container 100 and surrounding the channel 110; the flexible application assembly 200 includes an application zone 210 for defining a permeation site and a non-permeation zone 220 formed in connection with the protruding outer wall 300 of the container 100.

As shown in fig. 3, in the absence of force while the device is in a static state, the side of the flexible applicator assembly 200 overlying the container 100 adjacent the container 100 and the side of the channel 110 adjacent the flexible applicator assembly 200 are spaced apart from each other and define at least one cavity 400.

When the device is subjected to an axial force, the flexible applicator assembly 200 covering the container 100 approaches the channel 110 by squeezing the cavity 400 to reduce the volume of the cavity 400, and the side of the flexible applicator assembly 200 adjacent the container 100 receives the composition squeezed from the channel 110 by the gas in the cavity 400 by making the thickness of the axial portion of the flexible applicator assembly 200 smaller than the thickness of the channel 110 in the axial direction. When the liquid in the container 100 is depleted, the composition is able to flow from the channel 110 to the cavity 400 based on gravity or other external forces and be absorbed by the flexible applicator assembly 200 based on the change in volume of the cavity 400, based on the tilt angle of the container 100. When the flexible application assembly 200 absorbs excessive composition, the excessive composition can be drained to both sides of the flexible application assembly 200 based on the drainage function of the non-permeable region 220 formed by being connected to the protruding outer wall 300 of the container 100, so that the problem of uneven content of the composition in different regions of the flexible application assembly 200 does not occur when applying.

The flexible application assembly 200 is provided to be suitable for the curved sides of the bent roses with different degrees of curvature, and a user only needs to apply a device loaded with the composition to the curved sides of the bent roses in a pressing manner, so that the composition application process of the cut rose is simple to operate, and the application process using cotton swabs is prevented from becoming complicated and damaging the surfaces of the pedicel of the rose due to the loss of force. Application of the composition after damaging the surface of the pedicel of the chinese rose results in uncontrollable drug effects, thereby causing serious adverse effects on the growth of the chinese rose.

Example 7

This example was carried out in substantially the same manner as in example 1, example 2, example 3, example 4, example 5 or example 6, except for the following.

The phenomenon of rose benders can simultaneously produce a growing malformed sepal (BPP). The gene expression of auxin, cytokinin and gibberellin signal pathways in the malformed sepals and normal sepals is analyzed by experiments. In the malformed sepals, the expression of both the auxin biosynthesis-related gene RhTAR2 and the auxin-responsive gene RhGH3 was significantly up-regulated. RhKX 1 encodes a protein that catalyzes the degradation of cytokinins, the transcription level of which is also significantly elevated in deformed sepals, whereas the transcription level of the cytokinin-synthesizing positive regulator RhT5L1 is lower in deformed sepals. In addition, the expression level of two genes RhGA20-ox and RhGA3-ox involved in gibberellin anabolism in the deformed sepals is reduced. The above results indicate that the signal transduction of auxin, cytokinin and gibberellin in the malformed sepals is different from that of normal sepals. Based on the influence of sepals on the elbow phenomenon, elbow roses with consistent bending angles are selected, the deformed sepals are removed to serve as an experimental group, and the elbow roses without the sepals are taken as a control group. After 3 days, the elbow angle of the experimental group is greatly reduced relative to that of the control group, so that the difference of the hormone signal transduction strength of the deformed sepals and the normal sepals is verified, cell division and cell expansion on two sides of the pedicel are uneven, and finally the elbow phenomenon is caused.

Based on the above findings, the method of applying the plant growth regulator composition of the present invention comprises an application site of a plant, the application site comprising at least a pedicel and sepal of the plant.

The specific application method comprises the following steps:

selecting a plant individual plant with an elbow, and uniformly coating the plant growth regulator composition on the bent side of the pedicel of the selected elbow rose by using a device carrying the plant growth regulator composition.

And observing the recovery condition of the single plant of the plant 24 hours after the plant is coated, and if the curvature does not reach the expected effect, uniformly coating the plant growth regulator composition on the curved side of the pedicel of the selected elbow rose by using the device carrying the plant growth regulator composition again for observation.

The time interval between the front application and the back application is at least longer than 12 hours. The number of applications of the plant growth regulator can be determined based on the observation of the recovery condition of the applied plant individual, i.e., repeatedly applying the plant growth regulator to the bent side of the pedicel of the bent rose at appropriate intervals until the bent condition of the plant individual is recovered to the growth state of the normal plant individual.

Example 8

This example was carried out in substantially the same manner as in example 1, example 2, example 3, example 4, example 5, example 6 or example 7 except for the following.

The invention adopts 100-200mg/L naphthylacetic acid, 5-30 mg/L6-benzylamino adenine, 5-30mg/L gibberellin, 0.5-1.0mg/L plant amino acid, and prepares the composition for regulating plant growth by taking 200g/L lanolin as a carrier and absolute ethyl alcohol and distilled water as solvents.

The preparation method comprises the following steps: naphthalene acetic acid, 6-benzylamino adenine, gibberellin and plant amino acid are respectively measured according to the components of the formula, and the plant growth regulator needs to be dissolved by absolute ethyl alcohol. Heating and dissolving lanolin, adding water into a volumetric flask, then sequentially adding the raw materials, adding water to a constant volume of 1000ml, and violently shaking to fully mix the raw materials.

The formulated composition was applied to the curved side of the peduncle of the bent rose, and the recovery of the bent rose was observed.

The specific application method comprises the following steps:

selecting a variety of cut flower Chinese rose 'nectarine snow mountain', selecting 30 bent cut flower Chinese roses in a field, removing deformed sepals of the bent cut flower Chinese roses, uniformly coating a regulator on the bent side of a stem of the bent by using a cotton swab, and immediately photographing after coating.

After 24 hours, observation and photographing were performed again. Each applied rose photograph was collected and statistics were made of the percentage of roses treated to have significant effect.

The results show that the composition has good effect on correcting the elbow of the rose.

It should be noted that the above-mentioned embodiments are exemplary, and that those skilled in the art, having benefit of the present disclosure, may devise various arrangements that are within the scope of the present disclosure and that fall within the scope of the invention. It should be understood by those skilled in the art that the present specification and figures are illustrative only and are not limiting upon the claims. The scope of the invention is defined by the claims and their equivalents. The present description contains several inventive concepts, such as "preferably", "according to a preferred embodiment" or "optionally", each indicating that the respective paragraph discloses a separate concept, the applicant reserves the right to submit divisional applications according to each inventive concept. Throughout this document, the features referred to as "preferably" are only an optional feature and should not be understood as necessarily requiring that such applicant reserves the right to disclaim or delete the associated preferred feature at any time.

Claims

1. A plant growth regulator composition comprising a first component that induces a promoter response in response to cell division, a second component that is involved in the regulation of the transcriptional level of cell development in response to cell development, and a third component that provides a base material for vital activities in which the first and second components are involved.

2. The composition of claim 1, wherein the third component is a plant amino acid involved in a vital activity of cell division or growth.

3. The composition of claim 2, wherein the first component is tricyclic adenine that is stable in an acidic environment and capable of acting on plant stalks or side branches.

4. A composition according to claim 3, wherein the second component comprises one or more tetracyclic diterpene compounds and isomers thereof.

5. The composition of claim 4, wherein the composition is an alcohol dispersion comprising the first, second, and third components, wherein the alcohol dispersion comprises water to reduce the concentration of alcohol to reduce the corrosivity of the alcohol to plant tissue.

6. The composition of claim 5, wherein the alcoholic dispersion comprises at least one ester and a carrier for stabilizing the organic material.

7. The composition of claim 6, wherein the first component is present in an amount greater than the third component, and the second component is present in an amount greater than the third component.

8. A method of preparing a plant growth regulator composition, comprising the steps of:

adding the second component into a solvent consisting of a low concentration of an alcohol;

adding the first component and the third component;

and after the mixed solution is clarified, adding the carrier, and adjusting the pH of the solution to be weakly acidic to obtain the plant growth regulator composition acting on the elbow China rose.

9. A method for solving elbow of cut rose characterized by applying the composition of any one of claims 1 to 7 to rose having elbow phenotype.

10. The method of claim 9, comprising the steps of:

selecting qualified roses with elbow phenotypes;

determining the bending part of the rose with the elbow phenotype;

applying the composition of any one of claims 1-7 to a curved side of a curved area.