KR100525314B1 - Suspension for artificial pollination - Google Patents

Abstract

The present invention relates to a suspension for artificial moisture, per 1 liter of distilled water, sucrose 1-40 g, boric acid 0.1-150 mg, calcium nitrate 0.1-110 mg, sodium chloride 0.1-350 mg, gum arabic 0.1-550 mg and surfactant GDO 0.1-21 mg are mixed. Suspension for artificial moisture of the present invention is a new pollen extender that can be easily used by drastically reducing the labor force required for artificial moisture of horticultural crops, maintaining the vitality of the pollen to increase the fruiting rate and improve the suspension when dilution of the pollen In addition, by further mixing 0.1 ~ 40ml of ethyl alcohol artificially delay the germination of pollen can extend the use time as an extender for artificial moisture.

Description

Suspension for artificial pollination

The present invention relates to a suspension for artificial moisture, and more specifically, 1 to 40 g of sucrose, 0.1 to 150 mg of boric acid, 0.1 to 110 mg of calcium nitrate, 0.1 to 350 mg of sodium chloride, 0.1 to 550 mg of gum arabic and 0.1 to GDO of surfactant per liter of distilled water. It is a new type of pollen extender containing 21mg, which is a simple way of diluting pollen to maintain the vitality of the pollen and improve the suspension, and add 0.1 ~ 40ml of ethyl alcohol to induce germination delay to prolong the use time. It relates to a suspension for artificial moisture which is effective to.

Artificial moisture is artificially moisturized in order to bear fruit of fruit trees or horticulture plants, and is used when the number of insects that carry water of fruit trees is small or water is not close. When vegetables have a bad climate and insects do not fly, artificial moisture may be applied to watermelons and pumpkins.

In order to more efficiently use a limited amount of pollen during such artificial pollination, there are many cases where a pollen extender is mixed, but pollen extenders such as calendula, skim milk powder, and starch powder are known. However, such a powder extender had a hassle to be applied to the flower coop daily by using a tool such as a cotton swab after mixing with a flower pot.

On the other hand, the pollen extender in the form of a suspension is a polynade (pollenaid), an extender for sesame artificial moisture, which is manufactured in New Zealand and used in farms. In Korea, it was imported from the Korean Champaign Distribution Division (Hannam-gun Jeollanam-do) and supplied to farmers, but it was difficult to import, and the price was high. There is this. In addition, since the time available for artificial moisture after dilution is short within 2 hours, farmers need to dilute the pollen at least four times a day, which requires a lot of labor, and the vitality of the pollen increases as time passes after the dilution. Due to the lack of fertilization due to the lack of fertilizers or malformed fruit due to a lot of commodity rate falls.

Accordingly, the present inventors, as a result of intensive research to solve the above problems, while mixing a specific organic or inorganic material in distilled water, through a series of experiments to determine the effective amount of each substance to prepare a suspension to maintain the vitality of the pot The present invention was completed by increasing the fruiting rate and product yield rate, improving the suspension upon dilution of pollen, artificially delaying germination of pollen, and extending the use time as an extender for artificial pollination.

Accordingly, an object of the present invention is to provide a suspension for artificial moisture that can be used simply by maintaining the vitality of the pot, improving the suspension and extending the use time after dilution.

In order to achieve the above object, the suspension for artificial moisture of the present invention is 1-40 g of sucrose (sucrose) per 0.1 liter of distilled water, 0.1-150 mg of boric acid, 0.1-110 mg of calcium nitrate (calcium nitrate), sodium chloride A) 0.1 to 350 mg, arabic gum 0.1 to 550 mg, and a surfactant GDO 0.1 to 21 mg are mixed.

At this time, in order to artificially delay the germination of the pollen to prolong the use time as an artificial moisture extender, 0.1 to 40 ml of ethyl alcohol is further included, and more preferably, 10 to 20 ml of ethyl alcohol is mixed. .

In the present invention as described above, in particular, in order to maintain the vitality of the potted plant, the sucrose content is 10-20 g, the boric acid content is 50-100 mg, the calcium nitrate content is 30-70 mg, and the sodium chloride content is 150-. 250 mg is preferred.

On the other hand, the composition of the artificial moisture suspension of the present invention for improving the suspension of pollen is the content of the sodium chloride is 150 ~ 250mg, the content of the gum arabic is 150 ~ 350mg, the content of the surfactant GDO is 7 ~ 14mg It is preferable.

On the other hand, the suspension of the present invention is characterized in that it is commercially available in 10 to 30 times concentrated packaging.

Hereinafter, the present invention will be described in more detail.

To complete the present invention, first determine the appropriate concentration by comprehensively investigating the effect of the concentration of organic and inorganic materials to be mixed on the pollen vitality such as germination rate, breakage rate, the effect on suspension and the germination delay The pollen suspension was completed, and pollen germination rate, breakage rate and pollen tube length were examined according to the time of inoculation into the suspension. After dilution of the pollen, the sprayer was sprayed on the head of the flower to investigate the fruiting rate, overweight distribution and seed number. It was.

Suspension for artificial moisture of the present invention is characterized in that 1 to 40 g of sucrose, 0.1 to 150 mg of boric acid, 0.1 to 110 mg of calcium nitrate, 0.1 to 350 mg of sodium chloride, 0.1 to 550 mg of gum arabic and 0.1 to 21 mg of surfactant GDO per 1 L of distilled water. do.

On the other hand, the suspension of the present invention can adjust the content of certain components in the suspension or mix additional components in order to achieve the special purpose of maintaining the vitality of the pot, improving the suspension of the pot or temporary delay of germination, In order to maintain the vitality of the sucrose content of 10-20g, the boric acid content of 50-100mg, the calcium nitrate content of 30-70mg, the sodium chloride content of 150-250mg is preferable, In order to improve the softness, the sodium chloride content is 150-250mg, the gum content of arabic rubber is 150-350mg, and the surfactant GDO content is 7-14mg. Finally, the germination of pollen is artificially delayed. In order to extend the use time as an extender for artificial moisture, 0.1 to 40 ml of ethyl alcohol is further mixed, more preferably 10 to 20 ml of ethyl. It is recommended to mix the alcohol.

However, in such a change of content, when the content of various organic and inorganic components increases, the suspension and germination delay is improved, but the vitality of the pollen is lowered, so that the fertilization at the time of artificial moisture is poor, on the contrary, the content of organic or inorganic components is low. If the plant is not mixed or mixed at all, the plant's vitality, suspension and germination delay are all reduced, making it difficult to dilute and spray the pollen in suspension, making it difficult to obtain fertilization effect by artificial moisture.

On the other hand, the suspension of the present invention is preferably commercially packaged concentrated concentration of 10 to 30 times when commercialized. In addition, in the artificial moisture method using the suspension of the present invention, first, after mixing the pollen 3 to 5g per 1L of the suspension of the present invention, it is good to spray on the head of the flower using a manual sprayer or an ultrafast atomizer. This method of moisture can drastically reduce the labor required for artificial pollination of horticultural crops by simply spraying a diluted pollen suspension away from the hassle of having to bury a flower in the front of a flower using cotton swabs. .

Meanwhile, organic or inorganic substances such as potassium nitrate (KNO ₃ ), magnesium sulfate (MgSO ₄ ), carboxy methyl cellulose (CMC), and mannitol may be used for the suspension of the present invention. However, these substances are less involved in pollen germination and kidneys than the components that make up the suspension of the present invention. In addition, HCD, SFT, CFA, CO, OA, etc. may be used as the surfactant, but the pollen germination rate is lower and the pollen breakage rate tends to increase compared to GDO.

Hereinafter, the configuration and operation effects of the present invention through the examples will be described in more detail. However, it is apparent to those skilled in the art that the present invention is not limited only to these examples.

<Examples 1-3 and Comparative Example 1>

Preparation of suspensions with varying amounts of sucrose

Take 1 L of distilled water each, add 100 mg of boric acid, 70 mg of calcium nitrate, 200 mg of sodium chloride, 350 mg of gum arabic, and 14 mg of surfactant GDO (koremul-GDO-150), and add sucrose in different amounts of 10, 20, and 30 g each. After sufficiently stirring to complete the suspension for artificial moisture (Examples 1 to 3). In addition, a suspension (Comparative Example 1) to which no sucrose was added was also prepared.

<Examples 4 to 6 and Comparative Example 2>

Preparation of suspensions with varying amounts of boric acid

Take 1L of distilled water each, add 20g of sucrose, 70mg of calcium nitrate, 200mg of sodium chloride, 350mg of gum arabic and 14mg of surfactant GDO, and add 50, 100, 150mg of boric acid in different amounts and stir thoroughly For suspensions (Examples 4-6) were completed. In addition, a suspension in which no boric acid was added (Comparative Example 2) was also prepared.

<Examples 7 to 9 and Comparative Example 3>

Preparation of suspensions with varying amounts of calcium nitrate

Take 1L of distilled water each, add 20g of sucrose, 100mg of boric acid, 200mg of sodium chloride, 350mg of gum arabic and 14mg of surfactant GDO, and add calcium nitrate 30, 70, 110mg in different amounts and stir thoroughly For suspensions (Examples 7-9) were completed. In addition, a suspension in which no calcium nitrate was added (Comparative Example 3) was also prepared.

<Examples 10 to 13 and Comparative Example 4>

Preparation of suspensions with varying amounts of sodium chloride

Take 1L each of distilled water, add 20g of sucrose, 100mg of boric acid, 70mg of calcium nitrate, 350mg of gum arabic and 14mg of surfactant GDO, and add 50, 150, 250, 350mg of sodium chloride in different amounts and stir thoroughly. A suspension for artificial moisture (Examples 10-13) was completed. In addition, a suspension in which no sodium chloride was added (Comparative Example 4) was also prepared.

<Examples 14 to 16 and Comparative Example 5>

Preparation of suspensions with varying amount of gum arabic

Take 1L each of distilled water, add 20g of sucrose, 100mg of boric acid, 70mg of calcium nitrate, 200mg of sodium chloride, and 14mg of surfactant GDO, and add Arabic gum at 150, 350, 550mg, and then stir thoroughly. Suspension (Examples 14-16) was completed. In addition, a suspension (Comparative Example 5) in which no Arabic rubber was added was also prepared.

<Examples 17-19 and Comparative Example 6>

Preparation of Suspensions with Varying Amount of Surfactant GDO

Take 1L of distilled water each, add 20g of sucrose, 100mg of boric acid, 70mg of calcium nitrate, 200mg of sodium chloride, and 350mg of gum arabic, and add the surfactant GDO in varying amounts of 7, 14, 21mg each and then stir thoroughly Suspension (Examples 17-19) was completed. A suspension (Comparative Example 6) was also prepared in which no surfactant GDO was added.

<Examples 20 to 24>

Preparation of Suspension Further Mixing Ethyl Alcohol

20 g of sucrose, 100 mg of boric acid, 70 mg of calcium nitrate, 200 mg of sodium chloride, 350 mg of gum arabic, and 14 mg of surfactant GDO were added to 1 L of distilled water, followed by stirring sufficiently to complete a suspension for artificial moisture (Example 20). On the other hand, in order to delay germination of pollen, ethyl alcohol is additionally added to the suspension, but the amount of each of 10, 20, 30, and 40 ml is added in different amounts, followed by sufficient stirring to complete the suspension for artificial moisture (Examples 21 to 24). It was.

Experimental Example 1

Determination of Sucrose Addition to Maintain Vitality of Potted Plants

In the suspension for artificial pollination of the present invention, in order to determine the amount of sucrose that is desirable to maintain the vitality of the pollen, after dipping 20mg each of the potted persimmon pollen in 10ml of each suspension of Examples 1 to 3 and Comparative Example 1 After culturing in a thermostat at 0 ° C., about 15 μl of the cultured pollen suspension was evenly placed on a disposable slide glass and covered with a cover glass, and the germination of the pollen tube was longer than the diameter of the hydrated pollen (20 μm). The number of pollen and the length of pollen tube damaged during the cultivation were measured using an optical microscope (magnification: 200 times) using 100 to 150 pots observed at 7 to 10 points using an image analyzer (IMT, Korea). The immersion time (1, 2, 3, 4, 5 hours) was investigated for each, and the results are shown in Tables 1 to 3 below. As shown in Tables 1 to 3, Examples 1 to 3 to which sucrose was added were better than pollen germination and pot length of pollen tube compared to Comparative Example 1, which did not add any conventional polynade or sucrose, and thus the pollen breakage rate was lower. The vitality of the pollen was enhanced, and the longer the immersion time, the better the effect. Example 2 was the most excellent in the amount of sucrose.

Sucrose Addition and Pollen Germination Rate by Soaking Time Treatment (Sucrose) Pollen germination rate by immersion time (%) One 2 3 4 5 hours Pollenaid 31.9 43.3 57.9 63.5 65.6 Comparative Example 1 36.4 48.3 50.0 61.0 62.6 Example 1 36.2 55.3 68.4 73.1 78.8 Example 2 32.5 58.0 76.4 81.1 86.3 Example 3 32.9 51.8 66.1 77.5 79.5

Sucrose addition and pollen tube elongation by immersion time Treatment (Sucrose) Pollen pipe elongation by immersion time (㎛) One 2 3 4 5 hours Pollenaid 24.5 ± 2.8 43.6 ± 9.7 65.6 ± 14.4 76.0 ± 18.1 73.5 ± 23.9 Comparative Example 1 26.5 ± 5.5 31.9 ± 12.8 42.5 ± 16.2 73.8 ± 27.1 74.9 ± 38.1 Example 1 30.2 ± 9.6 54.7 ± 22.6 65.4 ± 29.0 98.7 ± 30.5 101.1 ± 35.5 Example 2 27.9 ± 7.6 64.4 ± 15.8 78.1 ± 29.7 121.9 ± 31.7 137.3 ± 42.4 Example 3 28.7 ± 5.9 56.5 ± 29.3 77.5 ± 36.3 127.6 ± 43.2 135.4 ± 49.9

Sucrose Breakdown Rate by Sucrose Content and Soaking Time Treatment (Sucrose) Pollen breakage rate by immersion time (%) One 2 3 4 5 hours Pollenaid 4.1 5.9 5.0 8.1 a ^z 8.0 a Comparative Example 1 2.0 4.0 4.0 5.0 b 6.0 b Example 1 2.0 2.0 4.0 3.9 bc 6.0 b Example 2 2.2 1.9 2.0 3.9 bc 6.0 b Example 3 2.0 4.1 2.0 2.0 c 2.0 c

^z Mean separation within columns by Duncan's multiple range test at 5% level

Experimental Example 2

Determination of Addition of Boric Acid to Maintain Vitality of Potted Plants

In the suspension for artificial moisture of the present invention, in order to determine the amount of boric acid that is desirable to maintain the vitality of the pollen, 10 ml of each suspension of Examples 4 to 6 and Comparative Example 2 was irradiated in the same manner as in Experiment 1 The results are shown in Tables 4 to 6 below. As shown in Tables 4 to 6, Examples 4 to 6 to which boric acid was added are better in pollen germination rate and pollen tube elongation than in the conventional polynade or Comparative Example 2, and in particular, the potted plant breakage rate is low, so that the vitality of the pollen is enhanced as a whole. It was found that the longer the immersion time, the better the effect. Example 5 was the best according to the amount of boric acid added.

Boric Acid Content and Pollen Germination Rate by Soaking Time Treatment (boric acid) Pollen germination rate by immersion time (%) One 2 3 4 5 hours Pollenaid 34.6 40.9 55.0 57.4 61.2 Comparative Example 2 25.2 28.6 34.9 55.6 61.1 Example 4 31.3 43.8 61.9 75.8 77.6 Example 5 34.2 50.6 72.1 78.0 85.6 Example 6 29.7 35.7 62.5 67.9 69.8

Boric acid addition amount and dipping time Treatment (boric acid) Pollen pipe elongation by immersion time (㎛) One 2 3 4 5 hours Pollenaid 27.5 ± 6.6 39.5 ± 8.2 55.2 ± 9.0 66.9 ± 11.2 78.2 ± 15.4 Comparative Example 2 22.6 ± 2.0 35.9 ± 5.4 40.6 ± 12.5 54.2 ± 11.4 67.1 ± 13.0 Example 4 24.7 ± 3.1 57.8 ± 7.5 71.8 ± 12.5 105.4 ± 35.5 118.1 ± 49.1 Example 5 29.2 ± 8.6 65.2 ± 18.0 87.2 ± 31.0 129.5 ± 36.2 135.1 ± 47.0 Example 6 26.5 ± 4.1 53.3 ± 10.7 90.9 ± 28.2 103.1 ± 32.7 105.8 ± 46.8

Boric Acid Additives and Plant Breakage Rate by Soaking Time Treatment (boric acid) Pollen Breakage Rate by Soaking Time (%) One 2 3 4 5 hours Pollenaid 4.2 4.2 8.0 8.0 b ^z 9.5 b Comparative Example 2 0.0 4.0 8.5 14.3 a 12.0 a Example 4 0.0 4.0 4.2 4.3 c 4.5 c Example 5 0.0 0.0 4.2 4.3 c 4.0 c Example 6 0.0 4.0 4.3 4.3 c 4.2 c

^z Mean separation within columns by Duncan's multiple range test at 5% level

Experimental Example 3

Determination of Calcium Nitrate Addition to Maintain Vitality of Potted Plants

In the suspension for artificial moisture of the present invention, in order to determine the amount of calcium nitrate that is desirable to maintain the vitality of the pollen, for 10 hours of each of the suspensions of Examples 7 to 9 and Comparative Example 3 after 4 hours after pollen immersion Investigation was carried out in the same manner as in Experimental Example 1, and the results are shown in Table 7 below. As shown in Table 7, Examples 7 to 9 to which calcium nitrate was added showed better pollen germination rate and pollen tube elongation compared to conventional polyamide or Comparative Example 3. Example 8 was the best according to the amount of calcium nitrate added.

Pollen Germination Rate and Pollen Tube Elongation by Calcium Nitrate Addition Treatment (Calcium Nitrate) Pollen germination rate (%) Pollen tube elongation (㎛) Pollenaid 58.6 60.7 ± 20.3 Comparative Example 3 65.9 57.7 ± 25.7 Example 7 75.9 89.3 ± 37.0 Example 8 80.5 127.2 ± 40.9 Example 9 76.3 102.3 ± 39.8

Experimental Example 4

Determination of Sodium Chloride Addition to Maintain Vitality and Sustainability of Potted Plants

In the suspension for artificial pollination of the present invention, in order to determine the amount of sodium chloride that is desirable for maintaining the vitality of the pot and at the same time improving the suspension, after dipping the pot for each 10 ml of the suspension of Examples 10 to 13 and Comparative Example 4 After 4 hours, the same procedure as in Experiment 1 was carried out, and the results are shown in Table 8 below. As shown in Table 8, it was found that Examples 10 to 13 to which sodium chloride was added are superior to pollen germination and pollen tube elongation compared to the existing polyamide or Comparative Example 4. Examples 11 and 12 were excellent in the amount of sodium chloride added.

Pollen Germination Rate and Pollen Tube Elongation by Addition of Sodium Chloride Treatment (Sodium Chloride) Pollen germination rate (%) Pollen tube elongation (㎛) Pollenaid 58.6 64.7 ± 22.3 Comparative Example 4 63.9 102.5 ± 35.7 Example 10 78.1 106.6 ± 41.4 Example 11 77.4 121.2 ± 44.4 Example 12 74.9 120.9 ± 40.8 Example 13 69.3 104.6 ± 38.8

On the other hand, in order to investigate the suspension, in a 50 mL beaker, 10 mL of each suspension and 20 mg of potted fern are shaken and shaken every 5 minutes while shaking. Very good 5 points, good 4 points, normal 3 points, bad 2 The scores were very bad and the scores were evaluated by a five-point scale. As shown in Table 9 below, the more the sodium chloride is added, the more the susceptibility of the pollen is improved, but in consideration of the effect on the vitality of the pollen, Examples 11 to 12 will be good.

Suspension of Potted Plants by Addition of Sodium Chloride Treatment (Sodium Chloride) Suspension of pollen by dilution time * 5 10 15 20 minutes Pollenaid 1.0 1.7 2.3 3.3 Comparative Example 4 1.0 1.7 2.8 3.8 Example 10 1.0 1.8 3.0 4.0 Example 11 1.2 2.3 3.2 4.5 Example 12 1.8 2.8 3.8 4.7 Example 13 2.0 3.0 4.0 4.9

* Suspension degree-1: Not good, 5: Good

Experimental Example 5

Determination of Addition of Arabian Rubber to Improve the Susceptibility of Potted Plants

In the suspension for artificial pollination of the present invention, in order to determine the addition amount of the gum arabic which is preferable to improve the suspension of the pollen, the pollen germination rate and the pollen tube elongation with respect to 10 ml of the suspension of Examples 14 to 16 and Comparative Example 5 are After 4 hours, the irradiation was conducted in the same manner as in Experimental Example 1, and the suspension investigation was performed in the same manner as in Experimental Example 4. The results are shown in Tables 10 and 11 below. As shown in Table 10 and 11, the more arabic rubber was added, the more the suspension was improved, but the pollen germination rate and pollen tube elongation decreased. Therefore, Example 15 would be the best considering the effect of pots on vitality.

Pollen Germination Rate and Pollen Pipe Elongation by Addition of Arabian Rubber Treatment (Arabic Rubber) Pollen germination rate (%) Pollen tube elongation (㎛) Pollenaid 60.5 66.0 ± 20.1 Comparative Example 5 75.7 124.8 ± 46.1 Example 14 73.5 122.1 ± 44.0 Example 15 79.1 122.9 ± 41.7 Example 16 78.3 118.2 ± 57.0

Suspension of Potted Plants by Addition of Arabian Rubber Treatment (Arabic Rubber) Suspension of pollen by dilution time * 5 10 15 20 minutes Pollenaid 1.0 1.7 2.3 3.2 Comparative Example 5 1.7 2.5 3.3 4.0 Example 14 1.7 2.5 3.5 4.5 Example 15 1.7 2.7 3.5 4.5 Example 16 1.8 3.0 4.0 4.8

* Suspension degree-1: Not good, 5: Good

Experimental Example 6

Determination of Addition of Surfactant GDO to Improve Suspension of Potted Plants

In the suspension for artificial pollination of the present invention, in order to determine the addition amount of the surfactant GDO which is preferable to improve the suspension of pollen, pollen germination rate and pollen tube extension amount for 10 ml of the suspension of Examples 17 to 19 and Comparative Example 6 4 hours after immersion, the same procedure as in Experiment 1 was performed, and the suspension was examined in the same manner as in Experiment 4. The results are shown in Tables 12 and 13 below. As shown in Tables 12 and 13, the more the surfactant was added, the more the suspension was improved, but the pollen germination and pollen tube elongation decreased. Therefore, Example 18 would be the best considering the effect of pollen on vitality.

Pollen Germination Rate and Pollen Pipe Elongation by GDO Content Processing (GDO) Pollen germination rate (%) Pollen tube elongation (㎛) Pollenaid 63.6 67.2 ± 19.7 Comparative Example 6 85.7 132.8 ± 48.5 Example 17 80.5 131.6 ± 46.3 Example 18 79.2 126.7 ± 43.7 Example 19 58.3 88.1 ± 34.5

Suspension of Potted Plants by GDO Content Processing (GDO) Suspension of pollen by dilution time * 5 10 15 20 minutes Pollenaid 1.0 1.5 2.5 3.0 Comparative Example 6 1.0 1.5 2.5 3.5 Example 17 1.0 1.5 3.0 4.0 Example 18 1.0 2.0 3.5 4.5 Example 19 2.0 3.0 4.0 5.0

* Suspension degree-1: Not good, 5: Good

Experimental Example 7

Determination of Addition of Ethyl Alcohol for Temporary Germination Delay in Pollen

In the aqueous pollination suspension of the present invention, in order to determine a desirable amount of ethyl alcohol to induce a temporary germination delay of pollen and at the same time maintain the vitality of the pollen, pollen germination rate for each 10ml of the suspension of Examples 20 to 24, Pollen tube elongation and pollen breakage rate were examined in the same manner as in Experiment 1 when 4 hours after pollen immersion.

At this time, in order to confirm the change of pollen by ethyl alcohol, 0.1 mL of pollen suspension was immersed in a disposable petri dish containing 5 mL of BK medium for each immersion time, and then incubated in a thermostat at 30 ° C. for 15 hours, and the rate of pollen germination, breakage rate and Pollen tube length was investigated. Germination delay can be induced by the addition of ethyl alcohol, but the failure rate of pollen increases and it is healed on germination medium, and after the alcohol is volatilized, it is re- germinated again to confirm survival. As shown in Tables 14 and 15, Examples 21 or 22 would be good to maintain pollen vitality while inducing germination delays.

Pollen Germination Rate and Pollen Pipe Elongation by Ethyl Alcohol Addition Treatment (Ethyl Alcohol) Pollen germination rate (%) Pollen tube elongation (㎛) After immersion After volatilization After immersion After volatilization Example 20 80.0 90.4 115.9 ± 36.2 200≥ Example 21 36.6 86.3 49.2 ± 10.6 191.7 ± 31.8 Example 22 27.5 85.4 31.8 ± 8.5 189.1 ± 35.0 Example 23 25.9 66.1 24.2 ± 4.2 154.9 ± 34.9 Example 24 16.5 56.0 23.7 ± 6.0 134.6 ± 40.5

Pollen Breakage Rate by Addition of Ethyl Alcohol Treatment (Ethyl Alcohol) Pollen Breakage Rate (%) After immersion After volatilization Example 20 2.5 ± 1.4 7.1 ± 1.1 Example 21 4.1 ± 2.0 8.5 ± 1.8 Example 22 5.6 ± 2.1 9.6 ± 1.9 Example 23 8.9 ± 2.7 11.8 ± 3.0 Example 24 11.5 ± 3.0 14.2 ± 3.7

Experimental Example 8

Effect of Artificial Moisture by Suspension

In order to compare the effect of artificial moisture by the suspension of the present invention with the efficacy of the existing product, Heyward 8-year-old planted in the Nanpo fruit test plant test blasting net house was disclosed, in preparation for 20mL / L of imported polynade (pollenaid) After diluting to 4 g / L of pollen in the suspension of the invention, respectively, immersed in 100 mL per week using a manual sprayer three times at two-day intervals from two days after the flowering of the dahlia flowers (1, 2, 3, 4, 5 hours) ) The fruiting rate, fruit size, fruit weight, number of seeds, etc. were examined by three times of egg mass treatment.

In the fruiting rate, the fruiting fruit was examined 30 times after artificial pollination by 20 per repetition, divided by the number of treatments, and expressed as a percentage. The fruit size was measured in late June by measuring the longitudinal and transverse diameters and dividing the longitudinal diameters by the transverse diameters as percentages. The overweight and overweight distributions were harvested in November and examined 50 families per iteration. The number of seeds was examined by collecting 10 fruits per repetition closest to the average fruit weight of each treatment. As a result, as shown in Tables 16 and 17, the suspension of the present invention had a higher fruiting rate, a heavy weight, and an improved product yield compared to polyamide, but there was no difference in the number of seeds. However, it was found that the immersion time was heavy even when the suspension of the present invention was immersed, and the excess ratio was improved even in the heavy distribution.

Comparison of fruiting rate, heavy weight and seed number after artificial pollination process Penetration rate (%) (G) Seed count Product Type Immersion time Pollenaid One 100 113.1 a ^z 969 a 2 100 106.3 be 965 a 3 98.6 105.6 ce 1,004 a 4 98.2 100.5 ef 630 b 5 95.5 98.4 f 567 b Suspension of the Invention One 100 111.7 ab 1,019 a 2 100 108.7 ad 1,040 a 3 99.0 110.9 ac 987 a 4 97.0 104.8 de 609 b 5 96.0 101.0 ef 636 b

^z Mean separation within columns by Duncan's multiple range test at 5% level

Heavy distribution and commodity rate after artificial pollination process Overweight distribution (%) Commodity Rate Product Type Immersion time 80g or less 80-99 g More than 100g Pollenaid One 0 8.0 92.0 100 2 0 39.1 60.9 100 3 0 39.8 60.2 100 4 4.3 41.3 54.4 95.7 5 10.5 40.7 48.8 89.5 Suspension of the Invention One 0 22.7 77.3 100 2 0 16.0 84.0 100 3 0 13.0 87.0 100 4 2.7 39.3 58.0 97.3 5 6.4 43.1 50.5 93.6

* Product rate: 80 g or more

As described above, the present invention is an extender for artificial pollination in the form of a suspension used by diluting the pollen, which greatly reduces the labor force required for artificial pollination, and is easy to use, in particular, to maintain the vitality of the pollen to increase the fruiting rate and product yield. It is convenient to use in farmhouses by extending the usage time as artificial moisture extender to more than 3 hours after pot dilution by artificially delaying germination of pollen and artificially delaying germination of pollen.

On the other hand, while the above has been described with reference to the preferred embodiments of the present invention, those of ordinary skill in the art will be various within the scope of the present invention without departing from the spirit and scope of the present invention described in the claims below. It will be understood that modifications and changes can be made.

Claims (6)

Suspension for artificial moisture comprising 1 to 40 g of sucrose, 0.1 to 150 mg of boric acid, 0.1 to 110 mg of calcium nitrate, 0.1 to 350 mg of sodium chloride, 0.1 to 550 mg of gum arabic and 0.1 to 21 mg of GDO surfactant per liter of distilled water. The method of claim 1, Suspension for artificial moisture, characterized in that it further comprises 0.1 to 40ml of ethyl alcohol. The method of claim 1, Said sucrose is 10-20g, said boric acid 50-100mg, said calcium nitrate 30-70mg, said sodium chloride 150-250mg suspension for artificial moisture. The method of claim 1, Said sodium chloride is 150-250mg, said gum arabic is 150-350mg, The surfactant GDO is 7 ~ 14mg, The suspension for artificial moisture. The method of claim 2, Suspension for artificial moisture, characterized in that the amount of the ethyl alcohol is 10 ~ 20ml. The method according to any one of claims 1 to 5, Suspension for artificial moisture characterized in that the suspension is concentrated and packaged 10 to 30 times the market.