CN103155947B - Peony pollen germination agent and preparation method thereof - Google Patents
Peony pollen germination agent and preparation method thereof Download PDFInfo
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- CN103155947B CN103155947B CN201310126613.5A CN201310126613A CN103155947B CN 103155947 B CN103155947 B CN 103155947B CN 201310126613 A CN201310126613 A CN 201310126613A CN 103155947 B CN103155947 B CN 103155947B
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Abstract
The invention relates to a peony pollen germination agent and a preparation method thereof. Each liter of peony pollen germination agent comprises the following components: 0.04g of boric acid, 50g of cane sugar, 0.02g of rapin, 3g of agar, 0.02g of calcium chloride, 30 micro-milliliters of phytic acid, 0.06g of vitamin B2, 20 micro-milliliters of glycerol, 0.03g of urea and the balance of water. The peony pollen germination agent is simple in preparation method and low in cost and can be applied to an artificial hybridization method for improving peony breeding fecundity; and after the pollen germination solution is artificially coated, pollen germination environment conditions are created, the pollen tube growth is promoted, the pollen germination is accelerated, the male parent pollen germination rate is improved, and the obstacles before exomixis are reduced, so that the peony maturing rate is improved.
Description
Technical field
The present invention relates to a kind of germinant and preparation method thereof, be specifically related to a kind of peony pollen germination agent and preparation method thereof.
Background technology
Tree peony (Paeonia sect. Moutan), elegant and poised, color is splendid, is not only most popular ornamental flower, also has very important economy, culture and medical value.But China tree peony is faced with resource considerable damage and loss at present, pattern is abundant but incomplete, and variety type is single, and breeding technique falls behind, the problems such as basic research and application study weakness.If be still only drunk at the advantage on surface; be unaware of potential difficulty and crisis; on the international arena that current tree peony is developed; can only there be glorious history and undeserved reputation in China; we want, in time based on resources advantage, to strengthen breeding process, improve breeding efficiency; put forth effort to cultivate new, strange, special kind, the bright flower of this ancient tradition could be allowed to shine brand-new brilliance.
Crossbreeding is the longest use of current tree peony breeding is also the most effective means, current crossing work is mainly carried out between the Varieties of Peony of natural flower synchronization, gather fresh pollen and carry out parent material intermolecular hybrid, during pollination, paternal pollen is applied in maternal column cap by direct swab stick, then carry out bagging isolation, after the florescence, sack is removed, Routine Management.Often due to the maternal column cap problem such as little the or mucous secretion of secreting mucus, mucus amount is not delayed in hybridization, after causing paternal pollen to be applied in maternal column cap, because maternal column cap does not possess paternal pollen sprouting condition, paternal pollen death or pollen germination rate is caused to decline, thus impact fertilization, cause shaky, affect the crossbreed amount of gathering.
Summary of the invention
The present invention seeks to the deficiency for solving the problems of the technologies described above, there is provided a kind of peony pollen germination agent and preparation method thereof, described peony pollen germination agent can artificially create pollen germination environmental condition, accelerates peony pollen and sprouts, improve pollen germination rate, thus improve the ripening rate of tree peony.
The present invention for solving the problems of the technologies described above adopted technical scheme is: a kind of peony pollen germination agent, its raw material is by boric acid, sucrose, agar, brassin, calcium chloride, phytic acid, Cobastab
2,glycerine, urea and water form, and each raw material addition is: add boric acid 0.04g, sucrose 50g, brassin 0.02g, agar 3g, calcium chloride 0.02g, phytic acid 30ul, Cobastab in often liter of peony pollen germination agent
20.06g, glycerine 20 μ l, urea 0.03g, surplus is water.
To prepare 1L peony pollen germination agent, a kind of peony pollen germination agent is described below, its concrete preparation process is:
Step one, be that the balance of 1/100g takes 50g sucrose and pours in beaker by accuracy;
Step 2, to measure with graduated cylinder in beaker that 800ml distilled water joins described in step one, stir with glass bar and make it to dissolve completely, obtain sucrose solution;
Step 3, by accuracy be 1/10000 balance measure 0.04g boric acid, 0.02g brassin, 0.02g calcium chloride, 0.03g urea and 0.06g Cobastab successively
2join in the sucrose solution described in step 2, stir with glass bar, after making it to dissolve completely, obtain mixed solution a;
Step 4, draw 30 μ l phytic acid and 20 μ l glycerine successively with liquid-transfering gun, join in the mixed solution described in step 3, after being uniformly mixed with glass bar, obtain mixed solution b;
Step 5, take 3g agar, join in the mixed solution b described in step 4, be uniformly mixed with glass bar, add water and be settled to 1L, after again mixing, gained mixed solution c is the peony pollen germination agent prepared by the present invention, loaded sealed storage in Brown Glass Brown glass bottles and jars only, for subsequent use.
Beneficial effect
Peony pollen germination agent of the present invention, preparation method is simple, with low cost; This pollen germination agent for peony characteristic and design, through experimental results demonstrate under the acting in conjunction of each component, can promote that peony tube cell grows, effectively improving pollen germination rate.Use it for and improve tree peony crossbreeding fecundity artificial hybridization method, by people for smearing pollen germination liquid in maternal column cap, create peony pollen and sprout environmental condition, guiding paternal pollen is sprouted, promote pollen tube growth, improve paternal pollen germination rate, reduce crossbreeding fertilization hole link, greatly can improve the ripening rate of tree peony.
Peony pollen germination agent of the present invention, each composition safety and environmental protection, with the addition of phytic acid can as preservative, and one of itself composition being pollen, avoids the drawback of other preservatives, is conducive to ensureing the long-term preservation of peony pollen germination agent and the stability of pollen.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described:
A kind of peony pollen germination agent, its raw material is by boric acid, sucrose, agar, brassin, calcium chloride, phytic acid, Cobastab
2, glycerine, urea and water composition, each raw material addition is: add boric acid 0.04g, sucrose 50g, brassin 0.02g, agar 3g, calcium chloride 0.02g, phytic acid 30ul, Cobastab in often liter of peony pollen germination agent
20.06g, glycerine 20 μ l, urea 0.03g, surplus is water.
A kind of peony pollen germination agent, its concrete preparation process is:
Step one, be that the balance of 1/100g takes 50g sucrose and pours in beaker by accuracy;
Step 2, to measure with graduated cylinder in beaker that 800ml distilled water joins described in step one, stir with glass bar and make it to dissolve completely, obtain sucrose solution;
Step 3, by accuracy be 1/10000 balance measure 0.04g boric acid, 0.02g brassin, 0.02g calcium chloride, 0.03g urea and 0.06g Cobastab successively
2join in the sucrose solution described in step 2, stir with glass bar, after making it to dissolve completely, obtain mixed solution a;
Step 4, draw 30 μ l phytic acid and 20 μ l glycerine successively with liquid-transfering gun, join in the mixed solution described in step 3, after being uniformly mixed with glass bar, obtain mixed solution b;
Step 5, take 3g agar, join in the mixed solution b described in step 4, be uniformly mixed with glass bar, add water and be settled to 1L, after again mixing, gained mixed solution c is the peony pollen germination agent prepared by the present invention, loaded sealed storage in Brown Glass Brown glass bottles and jars only, for subsequent use.
Step 6, a kind of peony pollen germination agent, its concrete using method, is namely applied to the artificial hybridization improving tree peony breeding fecundity, comprises the following steps:
(1), at tree peony initial bloom stage, cut male parent peony flowers, at indoor removal petal and calyx, be placed on clean culture dish, be then placed in shady and cool ventilation place and carry out natural seasoning, when anther dehiscence loose powder, shake fresh idea gently, collect pollen in pollen bottle, and pollen bottle is stored in freezer compartment of refrigerator, the storage temperature arranging freezer compartment of refrigerator is between-17 to-13 DEG C, for subsequent use;
The requirement of male parent, for conveniently to have outstanding behaviours in florescence, pattern, flower pattern etc., has at least one to meet breeding objective requirement;
When needing to use the pollen stored, pollen bottle being taken out from freezer compartment of refrigerator, is transferred in the Medical heat-preserving box of built-in ice cube;
(2), at tree peony initial bloom stage, wipe out maternal part petal with scissors, but can not holder be injured, the flower pesticide of female parent is removed clean with tweezers, then dip a small amount of peony pollen germination agent of the present invention with medical cotton stick and be applied in maternal column cap, dip pollen with medicinal cotton swab, be evenly applied to stigma surface, ensure that often kind of pollen has special cotton swab, then bagging isolation on female parent, be enclosed within by isolation pocket on female parent, lower end is fixed on tree peony anthocaulus after closing up, and can not injure anthocaulus time fixing;
(3), on the corresponding branch of hybridization maternal plant hang up label, set up hybridization archives; Hybridization archive content should comprise: hybrid combination numbering, Parent title, pollen collection used or storage time, pollination date and hybrid seed harvest date and quantity;
(4), after peony forescence, isolation pocket is made a call to 8 ~ 10 through holes, retains isolation pocket, then observe and strengthen daily management, until fruit and seed development to a certain extent time, timely collecting.
On described isolation pocket punch the aperture of through hole between 0.2 ~ 0.3cm.
The superiority that can improve tree peony breeding fecundity aspect when peony pollen germination agent of the present invention is applied to the artificial hybridization of tree peony breeding fecundity is further illustrated below by experimental data:
Table 1 is 2011 does the data statistics of hybrid experiment, and the current year adopts traditional hybridization technique when hybridizing, and does not use peony pollen germination agent of the present invention.
Table 1 is as follows:
Statistics through last: the current year cohybridization flower number 463, crossbreed 2153 of gathering, average ripening rate is 4.65/piece.
Table 2 is 2012 does the data statistics of hybrid experiment, and the current year employs peony pollen germination agent of the present invention when hybridizing.
Table 2 is as follows:
Do 1732 flowers the current year altogether, crossbreed 19421 seeds of gathering, average ripening rate 11.21/piece.
By the statistical data of the cross experiment of 2 years, can find out, adopt the artificial hybridization method after peony pollen germination agent of the present invention, the ripening rate of tree peony has had raising by a relatively large margin, and excellent result, is convenient to utilization and extention.
Claims (1)
1. a peony pollen germination agent, is characterized in that: the raw material of peony pollen germination agent is by boric acid, sucrose, agar, brassin, calcium chloride, phytic acid, Cobastab
2,glycerine, urea and water form, and each raw material addition is: add boric acid 0.04g, sucrose 50g, brassin 0.02g, agar 3g, calcium chloride 0.02g, phytic acid 30ul, Cobastab in often liter of peony pollen germination agent
20.06g, glycerine 20 μ l, urea 0.03g, surplus is water; Described peony pollen germination agent, its concrete preparation process is:
Step one, be that the balance of 1/100g takes 50g sucrose and pours in beaker by accuracy;
Step 2, to measure with graduated cylinder in beaker that 800ml distilled water joins described in step one, stir with glass bar and make it to dissolve completely, obtain sucrose solution;
Step 3, by accuracy be 1/10000 balance measure 0.04g boric acid, 0.02g brassin, 0.02g calcium chloride, 0.03g urea and 0.06g Cobastab successively
2join in the sucrose solution described in step 2, stir with glass bar, after making it to dissolve completely, obtain mixed solution a;
Step 4, draw 30 μ l phytic acid and 20 μ l glycerine successively with liquid-transfering gun, join in the mixed solution described in step 3, after being uniformly mixed with glass bar, obtain mixed solution b;
Step 5, take 3g agar, join in the mixed solution b described in step 4, be uniformly mixed with glass bar, add water and be settled to 1L, after again mixing, gained mixed solution c is described peony pollen germination agent, loaded sealed storage in Brown Glass Brown glass bottles and jars only, for subsequent use.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310126613.5A CN103155947B (en) | 2013-04-12 | 2013-04-12 | Peony pollen germination agent and preparation method thereof |
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| CN103155947B true CN103155947B (en) | 2015-06-17 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964559A (en) * | 2016-10-19 | 2018-04-27 | 张楠 | A kind of peony pollen vigor germination in vitro method for measuring |
| CN109496834B (en) * | 2018-12-11 | 2022-03-22 | 新疆林业科学院扎木台造林试验站 | Almond liquid pollen and preparation method thereof |
| CN112210581A (en) * | 2020-09-21 | 2021-01-12 | 洛阳农林科学院 | Detection agent and detection method for pollen activity of Paeonia plant |
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| CN1947496A (en) * | 2005-10-10 | 2007-04-18 | 北京林业大学 | Parents composition method for distant hybrid of peony and Chinese herbaceous peony |
| CN101633902A (en) * | 2009-08-25 | 2010-01-27 | 商洛学院 | In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof |
| CN102599046A (en) * | 2012-03-16 | 2012-07-25 | 江苏省农业科学院 | Pear pollination solution, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100525314B1 (en) * | 2004-01-30 | 2005-11-02 | 전라남도 | Suspension for artificial pollination |
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|---|---|---|---|---|
| CN1947496A (en) * | 2005-10-10 | 2007-04-18 | 北京林业大学 | Parents composition method for distant hybrid of peony and Chinese herbaceous peony |
| CN101633902A (en) * | 2009-08-25 | 2010-01-27 | 商洛学院 | In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof |
| CN102618483A (en) * | 2012-03-14 | 2012-08-01 | 中国林业科学研究院林业研究所 | Cattleya pollen germination medium |
| CN102599046A (en) * | 2012-03-16 | 2012-07-25 | 江苏省农业科学院 | Pear pollination solution, and preparation method and application thereof |
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Inventor after: Wang Zhanying Inventor after: Yan Jinxiao Inventor after: Liu Zhenguo Inventor after: Wang Liping Inventor after: Zhang Yanhong Inventor after: Du Jijun Inventor after: Zhao Guodong Inventor after: Wang Erqiang Inventor after: Lu Lin Inventor after: Wang Xiaohui Inventor after: Liu Hongfan Inventor after: Liang Changan Inventor after: Pang Jingjing Inventor after: Guo Yazhen Inventor after: Han Kun Inventor before: Wang Zhanying Inventor before: Yan Jinxiao Inventor before: Zhang Hongquan Inventor before: Liu Zhenguo Inventor before: Zhang Yanhong Inventor before: Wang Erqiang Inventor before: Lu Lin Inventor before: Liu Hongfan Inventor before: Nie Shihuan Inventor before: Liang Changan Inventor before: Pang Jingjing Inventor before: Guo Yazhen Inventor before: Han Kun |
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Free format text: CORRECT: INVENTOR; FROM: WANG ZHANYING WANG ERQIANG LU LIN LIU HONGFAN NIE SHIHUAN LIANG CHANGAN PANG JINGJING GUO YAZHEN HAN KUN YAN JINXIAO ZHANG HONGQUAN LIU ZHENGUO ZHANG YANHONG TO: WANG ZHANYING WANG ERQIANG LU LIN WANG XIAOHUI LIU HONGFAN LIANG CHANGAN PANG JINGJING GUO YAZHEN HAN KUN YAN JINXIAO LIU ZHENGUO WANG LIPING ZHANG YANHONG DU JIJUN ZHAO GUODONG |
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